Lampiran 2 : Morfologi tanaman dan simplisia rimpang cabang dan rimpang
induk kunyit
Gambar tumbuhan kunyit (Curcuma longa L.)
Gambar rimpang induk kunyit (Curcuma longa L.)
Gambar induk kunyit segar (Curcuma longa L.)
Gambar simplisia rimpang induk kunyit (Curcuma longa L.)
Gambar rimpang cabang kunyit (Curcuma longa L.)
Gambar rimpang cabang kunyit segar (Curcuma longa L.)
Gambar simplisia rimpang cabang kunyit (Curcuma longa L.)
Lampiran 3 : Gambar minyak atsiri hasil destilasi air
Gambar minyak atsiri hasil destilasi air
Keterangan:
A. Minyak atsiri dari simplisia rimpang cabang kunyit
B. Minyak atsiri dari rimpang cabang kunyit segar
C. Minyak atsiri dari simplisia rimpang induk kunyit
D. Minyak atsiri dari rimpang induk kunyit segar
Lampiran 4 : Gambar alat-alat yang dipakai
Gambar alat Sthal
Gambar alat piknometer
Gambar alat GC-MS
Gambar alat destilasi air
Gambar alat refraktometer abbe
Lampiran 5 : Hasil pemeriksaan mikroskopik
1 2 3 4 5 6
Gambar hasil pemeriksaan mikroskopik serbuk simplisia rimpang cabang kunyit
(Curcuma longa L.)
Keterangan :
1. Butir-butir pati
2. Parenkim dengan sel-sel minyak atsiri
3. Periderm
4. Parenkim berisi butir pati
5. Penebalan kayu dengan dinding berbentuk spiral
6. Rambut penutup
1 2 3 4 5 6
Gambar hasil pemeriksaan mikroskopik serbuk simplisia rimpang induk kunyit
(Curcuma longa L.)
Keterangan :
1. Butir-butir pati
2. Periderm
3. Rambut penutup
4. Parenkim berisi butir pati
5. Parenkim dengan sel-sel minyak atsiri
6. Penebalan kayu dengan dinding berbentuk spiral
Kadar air =
100
%
sampel
Berat
air
Volume
×
Kadar air I =
100
%
7
,
98
%
012
,
5
8
,
1
2
,
2
=
×
−
Kadar air II =
100
%
7
,
98
%
008
,
5
2
,
2
6
,
2
−
×
=
Kadar air III =
100
%
7
,
99
%
005
,
5
6
,
2
0
,
3
−
×
=
Kadar air rata-rata =
7
,
98
%
3
%
99
,
7
%
98
,
7
%
98
,
7
+
+
=
B. Simplisia rimpang cabang kunyit
Kadar air =
100
%
sampel
Berat
air
Volume
×
Kadar air I =
100
%
5
,
99
%
001
,
5
9
,
1
2
,
2
−
×
=
Kadar air II =
100
%
5
,
99
%
001
,
5
2
,
2
5
,
2
=
×
−
Kadar air III =
100
%
5
,
99
%
005
,
5
5
,
2
8
,
2
=
×
−
Kadar air rata-rata =
5
,
99
%
3
%
99
,
5
%
99
,
5
%
99
,
5
+
+
=
Lampiran 7. Perhitungan penetapan kadar sari larut dalam air
No.
Berat sampel (g)
Volume awal (ml)
Volume akhir (ml)
1.
5,012
1,8
2,2
2.
5,008
2,2
2,6
3.
5,005
2,6
3,0
No.
Berat sampel (g)
Volume awal (ml)
Volume akhir (ml)
1.
5,001
1,9
2,2
2.
5,001
2,2
2,5
Kadar sari larut air =
100
%
20
100
sampel
Berat
kosong
cawan
berat
sari
cawan
Berat
−
×
×
A. Rimpang induk kunyit
Kadar sari I =
100
%
16
,
163
%
20
100
005
,
5
3780
,
47
47,5398
−
×
×
=
Kadar sari II =
100
%
17
,
324
%
20
100
013
,
5
1251
,
475
45,2988
−
×
×
=
Kadar sari III =
100
%
18
,
371
%
20
100
016
,
5
3402
,
46
46,5245
−
×
×
=
Kadar sari larut air rata-rata =
17
,
286
%
3
%
371
,
18
%
324
,
17
%
163
,
16
=
+
+
B. Rimpang cabang kunyit
No
Berat sampel (g)
Berat cawan kosong (g)
Berat cawan sari
(g)
1
5,005
47,3780
47,5398
2
5,013
45,1251
45,2988
Kadar sari I =
100
%
20
,
34
%
20
100
015
,
5
3880
,
47
47,5921
−
×
×
=
Kadar sari II =
100
%
17
,
91
%
20
100
000
,
5
1208
,
45
45,2999
−
×
×
=
Kadar sari III =
100
%
19
,
17
%
20
100
005
,
5
5872
,
47
47,7792
−
×
×
=
Kadar sari larut air rata-rata =
19
,
14
%
3
%
17
,
19
%
91
,
17
%
34
,
20
=
+
+
Lampiran 8. Perhitungan penetapan kadar sari larut dalam etanol
No
Berat sampel (g)
Berat cawan kosong (g)
Berat cawan sari
(g)
1
5,015
47,3880
47,5921
2
5,000
45,1208
45,2999
Kadar sari larut etanol =
100
%
20
100
sampel
Berat
kosong
cawan
berat
sari
cawan
Berat
−
×
×
A. Rimpang induk kunyit
Kadar sari I =
100
%
14
,
67
%
20
100
007
,
5
45,0810
45,2280
−
×
×
=
Kadar sari II =
100
%
14
,
87
%
20
100
007
,
5
43,0522
43,2011
−
×
×
=
Kadar sari III =
100
%
14
,
40
%
20
100
002
,
5
44,9461
45,0902
−
×
×
=
Kadar sari larut etanol rata-rata =
14
,
64
%
3
%
40
,
14
%
87
,
14
%
67
,
14
+
+
=
B. Rimpang cabang kunyit
No
Berat sampel (g)
Berat cawan kosong (g)
Berat cawan
sari (g)
1
5,007
45,0810
45,2280
2
5,007
43,0522
43,2011
Kadar sari I =
100
%
17
,
06
%
20
100
013
,
5
1721
,
45
45,3432
−
×
×
=
Kadar sari II =
100
%
15
,
51
%
20
100
003
,
5
0540
,
43
43,2092
−
×
×
=
Kadar sari III =
100
%
14
,
96
%
20
100
000
,
5
5102
,
42
42,6598
−
×
×
=
Kadar sari larut air rata-rata =
15
,
84
%
3
%
96
,
14
%
51
,
15
%
06
,
17
+
+
=
Lampiran 9. Perhitungan penetapan kadar abu total
A. Simplisia rimpang induk kunyit
No
Berat sampel (g)
Berat cawan kosong (g)
Berat cawan sari (g)
1
5,013
45,1721
45,3432
2
5,003
43,0540
43,2092
Kadar abu total =
100
%
sampel
Berat
abu
Berat
×
Kadar abu total I =
100
%
4
,
45
%
001
,
2
0,089
×
=
Kadar abu total II =
100
%
4
,
81
%
048
,
2
0,097
×
=
Kadar abu total III =
100
%
4
,
61
%
004
,
2
092
,
0
×
=
Kadar abu total rata-rata =
4
,
69
%
3
%
61
,
4
%
81
,
4
%
45
,
4
=
+
+
B. Simplisia rimpang cabang kunyit
No
Berat sampel (g)
Berat abu (g)
1.
2,001
0,089
2.
2,048
0,097
3.
2,004
0,092
Kadar abu total =
100
%
sampel
Berat
abu
Berat
×
Kadar abu total I =
100
%
6
,
29
%
018
,
2
0,127
×
=
Kadar abu total II =
100
%
6
,
22
%
008
,
2
0,125
×
=
Kadar abu total III =
100
%
6
,
27
%
008
,
2
126
,
0
×
=
Kadar abu total rata-rata =
6
,
26
%
3
%
27
,
6
%
22
,
6
%
29
,
6
=
+
+
Lampiran 10. Perhitungan penetapan kadar abu tidak larut asam
1.
2,018
0,127
2.
2,008
0,125
A. Simplisia rimpang induk kunyit
Kadar abu tidak larut asam =
100
%
sampel
Berat
abu
Berat
×
Kadar abu I =
100
%
1
,
95
%
001
,
2
0,039
×
=
Kadar abu II =
100
%
2
,
13
%
018
,
2
0,043
×
=
Kadar abu III =
100
%
2
,
04
%
004
,
2
041
,
0
×
=
Kadar abu tidak larut asam rata-rata =
2
,
04
%
3
%
04
,
2
%
13
,
2
%
95
,
1
+
+
=
B. Simplisia rimpang cabang kunyit
No
Berat sampel (g)
Berat abu (g)
1.
2,001
0,039
2.
2,018
0,043
Kadar abu tidak larut asam =
100
%
sampel
Berat
abu
Berat
×
Kadar abu I =
100
%
1
,
09
%
018
,
2
0,022
×
=
Kadar abu II =
100
%
0
,
90
%
008
,
2
0,018
×
=
Kadar abu III =
100
%
0
,
99
%
008
,
2
020
,
0
×
=
Kadar abu tidak larut asam rata-rata =
0
,
99
%
3
%
99
,
0
%
90
,
0
%
09
,
1
+
+
=
Lampiran 11. Penetapan kadar minyak atsiri
No
Berat sampel (g)
Berat abu (g)
1.
2,018
0,022
2.
2,008
0,018
Kadar minyak atsiri =
A. Simplisia Rimpang Induk Kunyit
Sampel I
Volume minyak atsiri = 0,42 ml
Berat sampel
= 10,001 g
Kadar minyak atsiri =
= 4,19 % v/b
Sampel II
Volume minyak atsiri = 0,44 ml
Berat sampel
= 10,001 g
Kadar minyak atsiri =
= 4,39 % v/b
Sampel III
Volume minyak atsiri = 0,44 ml
Berat sampel
= 10,004 g
Kadar minyak atsiri =
= 4,39 % v/b
Kadar minyak atsiri rata-rata
=
Sampel I
Volume minyak atsiri = 0,12 ml
Berat sampel
= 10,002 g
Kadar minyak atsiri =
= 1,19 % v/b
Sampel II
Volume minyak atsiri = 0,09 ml
Berat sampel
= 10,004 g
Kadar minyak atsiri =
= 0,89 % v/b
Sampel III
Volume minyak atsiri = 0,10 ml
Berat sampel
= 10,000 g
Kadar minyak atsiri =
= 1,00% v/b
Kadar minyak atsiri rata-rata
=
= 1,03 % v/b
Sampel I
Volume minyak atsiri = 0,32 ml
Berat sampel
= 10,002 g
Kadar minyak atsiri =
= 3,19 % v/b
Sampel II
Volume minyak atsiri = 0,34 ml
Berat sampel
= 10,015 g
Kadar minyak atsiri =
= 3,39 % v/b
Sampel III
Volume minyak atsiri = 0,34 ml
Berat sampel
= 10,008 g
Kadar minyak atsiri =
= 3,39% v/b
Kadar minyak atsiri rata-rata
=
Sampel I
Volume minyak atsiri = 0,06 ml
Berat sampel
= 10,006 g
Kadar minyak atsiri =
= 0,59 % v/b
Sampel II
Volume minyak atsiri = 0,07 ml
Berat sampel
= 10,086 g
Kadar minyak atsiri =
= 0,69 % v/b
Sampel III
Volume minyak atsiri = 0,06 ml
Berat sampel
= 10,002 g
Kadar minyak atsiri =
= 0,59 % v/b
Kadar minyak atsiri rata-rata
=
= 0,63 % v/b
A. Simplisia rimpang induk kunyit
Sampel I
= 1,5122
Sampel II
= 1,5122
Sampel III
= 1,5122
Indeks bias rata- rata =
=
1,5122
3
1,5122
1,5122
1,5122
+
+
B. Rimpang induk kunyit segar
Sampel I
= 1,5141
Sampel II
= 1,5141
Sampel III
= 1,5141
Indeks bias rata- rata =
=
1,5141
3
1,5141
1,5141
1,5141
+
+
C. Simplisia rimpang cabang kunyit
Sampel I
= 1,5040
Sampel II
= 1,5040
Sampel III
= 1,5040
Indeks bias rata- rata =
=
1,5040
3
1,5040
1,5040
1,5040
+
+
D. rimpang cabang kunyit segar
Sampel I
= 1,5138
Sampel II
= 1,5138
Sampel III
= 1,5138
Indeks bias rata- rata =
=
1,5138
3
1,5138
1,5138
1,5138
+
+
Bobot jenis minyak atsiri =
A
C
A
B
−
−
Keterangan : A : Bobot piknometer kosong
B : Bobot piknometer + minyak atsiri
C : Bobot piknometer + akuades
A. Simplisia rimpang induk kunyit
Bobot jenis minyak atsiri
I =
3300
,
6
3307
,
8
3300
,
6
1720
,
8
−
−
= 0,9206 b/b
Bobot jenis minyak atsiri
II =
3300
,
6
3307
,
8
3300
,
6
1718
,
8
−
−
= 0,9176 b/b
Bobot jenis minyak atsiri
III =
3300
,
6
3307
,
8
3300
,
6
1718
,
8
−
−
= 0,9176 b/b
Bobot jenis rata-rata
=
3
9176
,
0
9176
.
0
9206
,
0
+
+
= 0,9186 b/b
B. Rimpang induk kunyit segar
Bobot jenis minyak atsiri
I =
3300
,
6
3107
,
8
3300
,
6
1850
,
8
−
−
= 0,9365 b/b
Bobot jenis minyak atsiri
II =
3300
,
6
3107
,
8
3300
,
6
1850
,
8
−
−
= 0,9365 b/b
Bobot jenis minyak atsiri
III =
3300
,
6
3107
,
8
3300
,
6
1829
,
8
−
−
=0,9354 b/b
Bobot jenis rata-rata
=
3
9354
,
0
9365
,
0
9365
,
0
+
+
0,9361 b/b
No
Bobot piknometer
kosong (g)
Bobot piknometer +
minyak atsiri (g)
Bobot piknometer +
akuades (g)
1.
6,3300
8,1720
8,3307
2.
6,3300
8,1718
8,3307
3.
6,3300
8,1718
8,3307
No
Bobot piknometer
kosong (g)
Bobot piknometer +
minyak atsiri (g)
Bobot piknometer +
akuades (g)
1.
6,3300
8,1850
8,3107
2.
6,3300
8,1850
8,3107
3.
6,3300
8,1829
8,3107
Lampiran 13. (lanjutan)
Bobot jenis minyak atsiri =
A
C
A
B
−
−
Keterangan : A : Bobot piknometer kosong
B : Bobot piknometer + minyak atsiri
C : Bobot piknometer + akuades
C. Simplisia rimpang cabang kunyit
Bobot jenis minyak atsiri
I =
3300
,
6
3308
,
8
3300
,
6
1320
,
8
−
−
= 0,9097 b/b
Bobot jenis minyak atsiri
II =
3300
,
6
3308
,
8
3300
,
6
1319
,
8
−
−
= 0,9096 b/b
Bobot jenis minyak atsiri
III =
3300
,
6
3308
,
8
3300
,
6
1318
,
8
−
−
= 0,9096 b/b
Bobot jenis rata-rata
=
3
9096
,
0
9096
.
0
9097
,
0
+
+
= 0,9096 b/b
D. Rimpang cabang kunyit segar
Bobot jenis minyak atsiri
I =
3300
,
6
3108
,
8
3300
,
6
1689
,
8
−
−
= 0,9284 b/b
Bobot jenis minyak atsiri
II =
3300
,
6
3108
,
8
3300
,
6
1689
,
8
−
−
= 0,9284 b/b
Bobot jenis minyak atsiri
III =
3300
,
6
3108
,
8
3300
,
6
1688
,
8
−
−
=0,9283 b/b
Bobot jenis rata-rata
=
3
9283
,
0
9284
,
0
9284
,
0
+
+
0,9284 b/b
No
Bobot piknometer
kosong (g)
Bobot piknometer +
minyak atsiri (g)
Bobot piknometer +
akuades (g)
1.
6,3300
8,1320
8,3308
2.
6,3300
8,1319
8,3308
3.
6,3300
8,1318
8,3308
No
Bobot piknometer
kosong (g)
Bobot piknometer +
minyak atsiri (g)
Bobot piknometer +
akuades (g)
1.
6,3300
8,1689
8,3108
2.
6,3300
8,1689
8,3108
3.
6,3300
8,1688
8,3108
dipisahkan
Ditambah Na
2SO
4anhidrat
karakterisasi
Lampiran 14. Flowsheet isolasi minyak atsiri simplisia rimpang induk kunyit
dan simplisia rimpang cabang kunyit
Keterangan: * dengan proses yang sama dilakukan dengan sampel simplisia
rimpang cabang kunyit.
Rimpang Induk Kunyit *
Dibersihkan
Dikeringkan
Pemeriksaan
Makroskopik Simplisia
Dirajang
Didestilasi air
Serbuk Simplisia
Minyak Atsiri dan Air
dikarakterisasi
Minyak atsiri dengan
kemungkinan adanya air
Air
1. Mikroskopis
2. PK Air
3. PK Abu
• Total
• Tidak Larut Asam
4. PK Sari
• Larut air
• Larut etanol
5. PK Minyak Atsiri
Minyak atsiri
Na
2SO
4x H
2O
Dengan GC-MS
Parameter
Fisika
Analisis Komponen Kandungan
Kimia
Indeks Bias
Bobot Jenis
Kromatogram GC
Spektrum MS
Dihaluskan
dipisahkan
Ditambah Na
2SO
4anhidrat
karakterisasi
Lampiran 15. Flowsheet isolasi minyak atsiri rimpang induk kunyit segar dan
rimpang cabang kunyit segar.
Keterangan: * dengan proses yang sama dilakukan dengan rimpang cabang
kunyit segar.
Rimpang Induk Kunyit *
Dibersihkan
Dirajang
Didestilasi air
Minyak Atsiri dan Air
Air
Dengan GC-MS
Parameter
Fisika
Analisis
Kandungan kimia
Indeks Bias
Bobot Jenis
Kromatogram GC
Spektrum MS
Minyak atsiri dengan
kemungkinan adanya air
Lampiran 16. Gambar kromatogram komponen minyak atsiri rimpang induk
kunyit kering
Peak R.Time Area %
Name
1 1.449 2.11 Pentane, 3-methyl- (CAS) 3-Methylpentane $$
2 1.650 0.47 Hexane (CAS) n-Hexane $$ Skellysolve B $$ n-C6H14 $$ Esan 3 2.002 0.21 Cyclobutanol, 2-ethyl-(CAS) 2-Ethylcyclobutanol $$
4 3.643 0.33 ALPHA-PINENE,(-)-$$ Bicyclo(3.1.1)hept-2-ene,2,6,6 trime 5 4.397 0.21 Beta-Myrcene $$ 1,6-Octadiene, 7-methyl-3-methylene- (CAS)
6 4.668 2.54 ALPHA PHELLANDRENE $$
2-methyl-5-(1-methylethyl)
8 5.106 6.40 1,8- Cyneole $$ 2-Oxabicyclo (2.2.2)octane, 1,3,3-trimethyl-(CAS)
9 5.649 0.14 Gamma, Terpinene $$ 1,4-Cyclohexadiene, 1-methyl-4-1(1-methyl)
10 6.275 1.12 Cyclohexene, 1-methyl-4-(1-methylethylidene)-$$ p-Mentha 11 7.351 0.12 1e,4a-Dihydroxy-(DELTA.6,7)-cis-OCTALIN $$
12 8.144 0.28 3-Cyclohexen-1ol. 4-methyl-1-(1-methylethyl)- (CAS)4-Terpinn 13 8.398 0.31 1,4-Cycloheptadiene,6-(1-butenyl),(S-(Z)-(CAS) ECTOCAR 14 8.627 0.16 Verbenol $$ 4,6,6-Trimethylbicyclo-(3,1,1)-hept-3-en-2-ol $$ 15 14.586 0.42 Trans-Caryophyllene $$ Bicyclo (7.2.0)undec-4-ene, 4,11,11-trim 16 15.166 0.46 4,7,10,13,16,19-Docosahexaenoic acid, metyl ester, (all-Z)-$$ 17 15.397 0.10 Cis-alpha,-Bisabolene$$ 4-(1Z)-1,5-Dimethyl-1,4-hexadienyl 18 15.461 0.16 Trans-Caryophyllene$$ Bicyclo(7.2.0)undec-4-ene,4,11,11-trim
19 15.66 0.34 CROWEACIN$$
20 15.790 0.76 TRANS-ISOMYRISTICIN $$ 21 15.998 3.60 MYRISTICIN $$
22 16.419 2.79 Zingiberene $$ 1,3-Cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl) 23 16.774 1.07 Cis-Famesol $$ 2,6,10-Dodecatrien-1-ol,3,7,11,-trimethyl, (Z,E) 24 17.099 2.44 Alpha-Cedrol $$ 1H-3a,-7-Methanoazulen-6-ol-, octahydro-3,6,8 25 17.291 0.48 Trans –Caryophyllene $$ Bicyclo
(7..2.0)undec-4-ene,4,11,11-trim
26 17.654 0.46 (-)-Caryophyllene oxide $$ (-)-5-Oxatricyclo (8.2.0.0(4,6) dodec 27 17.707 0.20 1H-Benzocycloheptene, 2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimet 28 17.795 0.45 (+) Aromadendrene $$ 1H-Cycloprop(e)azulene, decahydro 1,1 29 18.094 1.46 DYHDROARYLCURCUMEN $$ Benzene, 1-(1,5-dimethylhe) 30 18.470 0.51 Tumerone $$ 2-Methyl-6-(4-methyl-1.3-cyclohexadien-1-yl)-2 31 18.563 0.80 DIEPI-ALPHA-CEDREN I $$ DIEPI-ALPHA-CEDREN-(1) 32 18.678 0.60 Zingiberene $$ 1,3-Cyclohexadiene, 5-(1,,5-dimethyl-4-hexenyl) 33 18.772 0.88 6-(p-Tolyl)-2-methyl-2-heptenol $$ (+,-)-E-Nuciferol $$ 2-Hepten 34 18.867 0.60 Zingiberene $$ 1,3-Cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl) 35 19.187 0.88 Cedren-13-ol, 8-$$ Cedr-8-en-13-ol # $$
36 19.415 0.40 Alpha-Longipinene $$ Tricyclo (5.4.0.0(2,8)undec-9-ene, 2,6,6 37 19.502 1.59 Lanceol, cis $$ (2E)-2-(4,7-Dimethyl-3,4,4a,5,6,8a-hexahydro 38 19.614 1.14 Longipinocarveol, trans- $$ 39 20.074 13.38 Ar-timerone $$ 2-Methyl-6-(4-methylphenyl)-2-hepten-4-one 40 20.346 25.63 Tumerone $$ 2-Methyl-6-(4-methyl-1,3-cyclohexadien-1-yl)-2- 41 20.999 11.15 Curlone $$ 42 21.848 1.30 (E)-4-(1’,2’-Epoxy-2’,6’,6’-trimethyl-1’-cyclohexyl)-2-methyl-1,3 43 22.114 1.10 Trans-alpha-Bergamotene $$ 2,6-Dimethyl-6-(4-methyl-3-pentene)
44 22.288 1.82 Propanedinitrile, dicyclohexyl-$$ 2,2- Dicyclohexylmalononitril 45 22.521 0.70
1H-Indene,2,3,3a,4,7,7a-hexahydro-2,2,,4,4,7,7-hexamethyl-(CAS)
46 22.834 1.01 Trans-Verbenol $$ Bicyclo (3.1.1)hept-3-en-2-ol, 4,6,6-trimethyl 47 23.284 1.20 Beta-Sinensal $$ 2,6,9,11-Dodecatetraenal, 2,6,10-trimethyl 48 23.924 0.57 BUTAN-1,1-DICARBONITRIL, 1-CYCLOHEXYL-3-METHYL 49 24.556 0.62 Tumerone $$ 2-Methyl-6-(4-methyl-1.3-cyclohexadien-1-yl)-2 50 25.032 0.34 2-Butenoic acid, 2-methyl, 2-(acetyloxyl)-1,1a,2,3,4,6,7,10,11 51 25.245 0.44 Propanedinitrile, dicyclohexyl- (CAS) DICYCLOHEYLMAL 52 25.454 0.42 1,7,7-Timethylbicyclo (2.2.1)hept-2-yl-3-methyl-2-butenoate $$ 53 26.569 0.99 2-Butenoic acid, 2-methyl-, 2-(acetyloxy)-1, 1a,2,3,4,6,7,10,11
Lampiran 17. Gambar kromatogram komponen minyak atsiri rimpang induk
kunyit segar
Peak R.Time Area% Name
1 1.449 1.68 Pentane, 3-methyl- (CAS) 3-Methylpentane $$ (C2H5)2CHCH3 2 1.647 0.23 Hexane (CAS) n-Hexane $$ Skellysolve B $$ n-C6H14 $$ Esan 3 2.002 0.16 1,3,5-Cycloheptatriene (CAS) Cycloheptatriene $$ Tropilidin $$ 4 3.646 0.10 ALPHA-PINENE,(-)-$$ Bicyclo(3.1.1)hept-2-ene,2,6,6 trime 5 4.399 0.08 Beta-Myrcene $$ 1,6-Octadiene, 7-methyl-3-methylene- (CAS) 6 4.668 1.32 1-Phellandrene $$ 1,3-Cyclohexadiene,
2-methyl-5-(1-methylethyl)
7 4.933 0.43 Benzene, 1-methyl-2-(1-methylethyl)-(CAS) 1-Methyl-2-isopro 8 5.094 2.07 1,8-Cineole $$ 2-Oxabicyclo (2.2.2)octane, 1,3,3-trimethyl-
9 5.652 0.09 Gamma-Terpinene $$ 1,4-Cyclohexadiene, 1-methyl-4-(1-methyl) 10 6.279 0.58 ALPHA TERPINOLENE $$ Cyclohexadiene,
1-methyl-4-(1-methyl)
11 8.148 0.10 3-Cyclohexen-1-ol, methyl-1-(1-methylethyl)- (CAS) 4-Terpinene
12 8.401 0.14 Gamma-Terpinene $$ 1,4-Cyclohexadiene, 1-methyl-4-(1-methyl) 13 14.590 0.34 Trans-Caryophyllene $$ Bicyclo (7.2.0)undec-4-ene,
4,11,11-trimethyl
14 15.172 0.39 DEHYDROAROMADENDRENE $$
15 15.043 0.06 Alpha Humulene $$ 1,4,8- Cycloundecatriene, 2,6,6,9-tetrameth
16 15.464 0.16 GERMACRENE-D $$
17 15.658 0.07 Alloaromadendrene $$ 1H-Cycloprop(e)azulene, decahydro-1,1 18 16.006 1.25 Benzene, 1-(1,5-dimethyl-4-hexenyl)-4-methyl- (CAS) ar-curcu 19 16.144 0.08 2(1H)-Naphthalenone, 7-ethynyl-4a,5,6,7,8,8a-hexahydro-1,4a-d 20 16.434 2.50 Zingiberene $$ 1,3-Cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl) 21 16.683 0.11 Cyclohexane, 1,5-diethyl-3-methyl-2-methylene, (1.alpha.,3a) 22 16.780 0.73 Beta-Bisabolene $$ Cyclohexene,
1-methyl-4-(5-methyl-1-methyl)
23 17.115 2.44 Beta- Sesquiphellandrene (CAS) 2-METHYL-6-(4-METHYLETHYL)
24 17.248 0.14 Cis-p-Mentha-1(7),8-dien-2-ol $$
25 17.299 0.24 Naphtalene,1,2,3,4,4a,5,6,8a-Octahydro-7-methyl-4-methylelene 26 17.661 0.43 o-Mentha -1(7)8-dien-3-ol (CAS)
CIS-ISOPROPENYL-2-METHYL
27 17.805 0.41 Beta –Ionol $$ 3-Buten-2-ol-, 4-(2,6,6-trimethyl-1-cyclohexen-1- 28 18.130 1.92 APHA SINENSAL $$ 2,6,10-TRIMETHYL,-2,6,11-DODEC 29 18.481 0.45 Propanedinitrile, dicyclohexenyl- (CAS)
DICYCLOHEXYLMALS
30 18.558 0.70 1H-3a, 7-Menthanoazulene, 2,3,4,7,8,8a-hexahydro-3,6,8,8-tetram 31 18.806 1.26 (+-)-2,3,6,7-tetramethyl-1,4,4a.alpha.,5,8,8a.,9.beta.,9a.alpha 32 18.876 2.13 Alpha.-Cedrol $$ 1H-3a,7-Methanoazulen-6-ol, octahydro-3,6,8 33 19.537 2.14 (-)-Caryophyllene oxide $$(-)5-Oxatricyclo(8.2.0.0(4,6)dodeca 34 19.626 2.40 Cyclobutene, 4,4-dimethyl-1-(2,7-octadienyl)-(CAS)
35 19.854 0.89 LONGIPINOCARVEOL, TRANS-$$
36 20.253 19.40 BETA TUMERONE $$
37 20.482 17.98 AR-TUMERONE $$
38 20.557 0.88 Alpha.Longipinene $$ Tricyclo(5.4.0.0(2,8)undec-9-ene,2,6,6 39 20.767 0.55 APHA SINENSAL $$ 2,6,10-TRIMETHYL,-2,6,9,11-DODEC 40 21.130 14.08 Alpha –Ionol $$ 3-Buten-2-ol, 4-(2,6,6-trimethyl-2-cyclohexen-1 41 21.899 1.51 2,6,10-Trimethylundecan-(5E)-2,5,9-trien-4-one $$
42 22.073 0.41 APHA SINENSAL $$ 2,6,10-TRIMETHYL,-2,6,11-DODEC 43 22.182 2.05 Zingiberene $$ 1,3-Cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl) 44 22.360 2.76 Propanedinitrile, dicyclohexenyl- (CAS)
DICYCLOHEXYLMALS
45 22.552 0.74 1H-Indene, 2,3,3a,4,7,7a-hexahydro-2,2,4,4,7,7-hexamethyl-(CAS)
46 22.865 1.55 (-)-Caryophyllene oxide $$(-)5-Oxatricyclo(8.2.0.0(4,6)dodeca 47 23.036 0.39 Bicyclo (2,2,2)oct-2-ene, 1,2,3,6-tetramethyl- (CAS)
48 23.334 2.06 SPIRO(BICYCLO(3.3.0)OCTAN -6-ON-3-CYCLOPROPAN) 49 23.687 0.23 1,7,7-Trimethylbicyclo (2.2.1)hept-2-yl-3-methyl-2-butenoate $$ 50 23.773 0.31 7-(1,3-DIMETHYL-BUTA-1,3-DIENYL)-1,6,6-TRIMETHYL 51 23.957 1.22 2,6,10-Trimethylundecan-(5E)-2,5,9-trien-4-one $$
52 24.192 0.28 3,4-DIISOPROPYL-2,5-DIMETHYL-3-HEXENE $$ 53 24.583 1.22 Propanedinitrile, dicyclohexenyl- (CAS)
54 24.813 0.28 2,6,10-Trimethylundecan-(5E)-2,5,9-trien-4-one $$ 55 25.070 0.54 Propanedinitrile, dicyclohexenyl- (CAS)
DICYCLOHEXYLMALS
56 25.280 0.68 3,4-DIISOPROPYL-2,5-DIMETHYL-3-HEXENE $$
57 25.494 0.88 3-HEXENE,2,5-DIMETHYL-3,4-BIS(1-METHYLETHYL)-$ 58 25.699 0.30 1,1-PROPANDICARBONITRILE,1,2-DICYCLOHEXYL-$$ 59 26.627 1.82 2,6,10-Trimethylundecan-(5E)-2,5,9-trien-4-one $$
Lampiran 18. Gambar kromatogram komponen minyak atsiri rimpang cabang
kering
Peak R.Time Area% Name
1 1.449 1.11 Hexane (CAS) n-Hexane $$ Skellysolve B $$ n-C6H14 $$ Esan 2 1.627 0.18 Hexane (CAS) n-Hexane $$ Skellysolve B $$ n-C6H14 $$ Esan 3 2.004 0.13 Benzene, methyl- (CAS) Toluene $$ CP 25 $$ Methylbenzene $ 4 3.649 0.55 ALPHA-PINENE,(-)-$$ Bicyclo(3.1.1)hept-2-ene,2,6,6 trime 5 4.164 0.06 Sabinene $$ Bicyclo (3.1.0)hexane,
4-methylene-1-(1-methylethyl)
8 4.706 4.36 1-Phellandrene $$ 1,3-Cyclohexadiene, 2-methyl-5-(1-methylethyl)
9 4.971 2.95 Benzene, 1-methyl-2-(1-methylethyl)-(CAS) 1-Methyl-2-isopro 10 5.150 6.34 1,8-Cineole $$ 2-Oxabicyclo (2.2.2)octane, 1,3,3-trimethyl-
(CAS)
11 5.665 0.77 Gamma-Terpinene $$ 1,4-Cyclohexadiene, 1-methyl-4-(1-methyl)
12 6.152 0.10 BENZENE, 1-ISOPROPENYL METHYL $$
13 6.309 2.58 Linalool $$ 1,6-Octadien-3-ol, 3,7,-dimethyl- (CAS) Linalool $$ 14 6.875 0.06 p-menth-2-en-1-ol $$
15 7.243 0.13 BUTAN-1,1-DICARBONITRIL,,1- CYCLOHEXYL-3-METHYL
16 7.355 0.11 Perilla alcohol $$ 1-Cyclohexene-1-methanol,4-(1-methylethyl) 17 7.796 0.10 3-cyclohexene-1-methanol,alpha,.alpha,4-trimethyl,(S)-(CAS) 18 8.158 0.46 1-4Terpineol $$ 3-Cyclohexen-1-ol, 4-methyl-1-(1-methylethyl) 19 8.412 0.39 Linalyl propionate $$ 1,6-Octadien-3-ol,2,7 dimethyl-propanoate 20 8.639 0.25 Verbenol $$ 4,6,6-Trimethylbicyclo-(3.1.1)hept-3-en-ol $$ 21 9.460 0.11 Limonene oxide $$ 7-Oxabicyclo(4.1.0)heptane,1-methyl- 22 10.606 0.11 (E)-SOLANONE $$ 6,8-Nonadien-2-one,8-methyl-5-(1-methyl) 23 10.856 0.29 Phenol,5-methyl-2-(1-methylethyl)-(CAS) Thymol $$ m-Tymol 24 11.079 0.09 3-Methyl-2-(2-methyl-2butenyl)-furan $$ Rosefuran $$ Furan 25 11.313 0.18 Bicyclo (3.1.1)heptane-2,3-diol,2,6,6-trimethyl- (CAS) PINANE 26 11.397 0.11 1-P-MENTHEN-8-YL ACETATE $$ 3-Cyclohexene 1-methanol
27 12.493 0.24 ALPHA TERPINENYL ACETATE $$
28 14.605 1.02 TRANSBETA)-CARYOPHYLLENE $$
29 15.180 0.54 Beta bisabolene $$ BETA-BISABOLENE $$ Cyclohexene 30 15.412 0.17 Alpha Humulene $$ 1,4,8-Cycloundecatriene,2,6,6,9-tetramethyl 31 15.470 0.20 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene- (CAS) 32 15.568 0.07 1H-Benzocycloheptene, 2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimet 33 15.658 0.10 (+) Aromadendrene $$ 1H-Cycloprop(e)azulene, decahydro 1,1 34 16.024 1.68 Benzene, 1-(1,5-dimethyl-4-hexenyl)-4-methyl- (CAS) ar-curcu 35 16.196 0.16 Trans-Caryophyllene $$ Bicyclo (7.2.0)undec-4-ene,
4,11,11-trimethyl
36 16.451 2.52 Zingiberene $$ 1,3-Cyclohexadiene, 5-(1,5-dimethyl-4-hexenyl) 37 16.690 0.09 Santalol (CAS)
38 16.791 0.77 Beta-Bisabolene $$ Cyclohexene, 1-methyl-4-(5-methyl-1-methyl)
39 17.136 2.62 Beta- Sesquiphellandrene (CAS) 2-METHYL-6-(4-METHYLETHYL)
40 17.256 0.14 Cyclohexanol, 2-methylene-5-(1-methylethenyl)-(CAS) p-Ment 41 17.309 0.27 1H-Benzocycloheptene, 2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimet 42 17.669 0.45 (-)-Caryophyllene oxide $$(-)5-Oxatricyclo(8.2.0.0(4,6)dodeca 43 17.729 0.19 Alpha.-Cedrol $$ 1H-3a,7-Methanoazulen-6-ol, octahydro-3,6,8 44 17.812 0.36 Beta –Ionol $$ 3-Buten-2-ol-, 4-(2,6,6-trimethyl-1-cyclohexen-1- 45 18.138 1.54 Propanedinitrile, dicyclohexenyl- (CAS)
DICYCLOHEXYLMALS
46 18.488 0.35 LONGIPINOCARVEOL, TRANS-$$ 47 18.587 0.75 HEPTANE, 6-METHYL-2-P-TOLYL-$$
48 18.703 0.66 Naphtalene,1,2,3,4,4a,5,6,8a-Octahydro-7-methyl-4-methylelene 49 18.809 0.65 (+-)-2,3,6,7-tetramethyl-1,4,4a.alpha.,5,8,8a.,9.beta.,9a.alpha 50 18.887 1.33 1A,9B-Dihydrocyclopropa (1) phenanthrene $$ 1H-Cyclopropa 51 19.148 0.96 Trans-Chrysanthenyl acetate $$ Bicyclo (3.1.1)hept.2-en-6-ol,2,7 52 19.441 0.42 EPI-ALPHA-PATSCHULENE $$
53 19.528 1.25 LONGIPINOCARVEOL, TRANS-$$
55 20.250 15.93 Propanedinitrile, dicyclohexenyl- (CAS) DICYCLOHEXYLMALS
56 20.471 15.89 AR-TUMERON
57 20.550 1.26 Benzene, 1-(1,5-dimethyl-4-hexenyl)-4-methyl- (CAS) ar-curcu 58 21.114 12.50 DELTA-NEOCLOVEN $$
59 21.890 1.18 2,6,10-Trimethylundecan-(5E)-2,5,9-trien-4-one $$
60 22.064 0.28 Alpha-Longipinene $$ Tricyclo(5.4.0.0(2,8)undec-9-one,2,6,6 61 22.335 1.83 Zingiberen $$ 1,3-Cyclohexadiene,5-(1,5-dimethyl-4-hexenyl) 62 22.335 1.83 Propanedinitrile, dicyclohexenyl- (CAS)
DICYCLOHEXYLMALS
63 22.561 1.14 1H-Indene,2,3,3a,4,7,7a-hexahydro-2,2,4,4,7,7-hexamethyl 64 22.847 0.96 ACRYLSAEURE, BORNYLESTER $$ 2-Propenoic acid,1,7,7 65 23.032 0.27 BICYCLO (5.2.0)NONAN, 4-METHYLEN-2,8,8-TRIMETYHL 66 23.315 1.09 2-butenoic acid,2-methyl,2-(acetyloxyl)-1,1a,2,3,4,6,7,10,11 67 23.945 0.40 2-butenoic acid,2-methyl,2-(acetyloxyl)-1,1a,2,3,4,6,7,10,11 68 24.567 0.56 ACETIC ACID 2-METHYLENE-BICYCLO (3.2.1)OCT-6-EN 69 24.808 0.19 2,6,10-Trimethylundecan-(5Z)-2,5,9-trien-4-one $$
70 25.052 0.31 Propanedinitrile, dicyclohexenyl- (CAS) DICYCLOHEXYLMALS
71 25.262 0.38 2-butenoic acid,2-methyl,2-(acetyloxyl)-1,1a,2,3,4,6,7,10,11 72 25.477 0.52 3,4-DIISOPROPYL-2,5-DIMETYHL-3-HEXENE $$ 73 26.589 0.65 3-HEXENE 2,5-DIMETHYL-3,4-BIS (1-METHYLETHYL) 74 26.726 0.33 Hexadecanoic acid (CAS) Palmitic acid $$ Palmitinic acid $$
Lampiran 19. Gambar kromatogram komponen minyak atsiri rimpang cabang
segar
Peak R.Tim e Area % Name1 1.449 1.48 Hexane (CAS) n-Hexane $$ Skellysolve B $$
2 1.646 0.64 Butanal,2-methyl-(CAS)2-Methylbutanal $$ ISOVALERALDEE 3 2.003 0.13 Benzene, methyl-(CAS) toluene $$ CP 25$$ Methylbenzene $$ 4 4.670 1.32 1-Phellandrene $$ 1,3- Cyclohexadiene, 2-methyl-5 (1-methylethyl) 5 4.934 0.48 Benzene, 1-methyl-2-(1-methylethyl)-(CAS) 1-Methyl-2-isopropyl 6 5.093 1.38 1,8-Cineole $$ 2-Oxabicyclo (2.2.2)octane, 1,3,3-trimethyl- (CAS) 7 5.655 0.09 Gamma-Terpinene $$ 1,4-Cyclohexadiene, 1-methyl-4-(1-methyl) 8 6.282 0.76 ALPHA TERPINOLENE $$ Cyclohexadiene, 1-methyl-4-(1-methyl) 9 8.151 0.09 3-Cyclohexen-1-ol, 4-methyl-1-(1-methylethyl)- (CAS) 4-Terpinene
10 8.404 0.10 CIS-SABINO; $$ Bicyclo (3.1.0)hexan-3-ol,4-methylene-1
11 10.847 0.11 Phenol, 2-methyll-5-(1-methylethyl)-(CAS)Carvacrol $$ 2-HYDRO 12 14.596 0.66 TRANS(BETA)-CARYOPHYLENE $$
13 15.174 0.36 DEHYDROAROMADENDRENE $$
14 15.467 0.12 Beta-cedrene $$ 1H-3a,7-Methanoazulene, octahydro-3,8,8 trimet 15 16.005 1.22 Benzene, 1-(1,5-dimethyl-4-hexenyl)-4-methyl- (CAS) ar-curcu 16 16.426 1.92 Zingiberen $$ 1,3-Cyclohexadiene,5-(1,5-dimethyl-4-hexenyl) 17 16.780 0.51 Beta-Bisabolene $$ Cyclohexene, 1-methyl-4-(5-methyl-1-methyl) 18 17.107 1.99 d-Nerolidol $$ 1,6,10-Dodecatrien-3-ol, 3,7,11-trimethyl,(S(Z) 19 17.298 0.26 Trans Caryophylene $$ Bicyclo (7.2.0)undec-4ene, 4,11,11 trimeth 20 17.661 0.32 o-Mentha-1(7)8-dien-3-ol (CAS) CIS-ISOPROPENYL-2-METHYL 21 17.719 0.15 CYCLOHEXANOL, 2-METHYLEN-5-ISOPROPENYL- $$ 22 17.805 0.33 (-)-Caryophyllene oxide $$(-)5-Oxatricyclo(8.2.0.0(4,6)dodeca 23 17.948 0.25 Beta –Ionol $$ 3-Buten-2-ol-, 4-(2,6,6-trimethyl-1-cyclohexen-1- 24 18.108 1.62 2,3 dibromo-8-phenyl-p-mentane $$ 25 18.232 0.33 Butan-1,1-dicarbonitril,1-cyclohexyl-3-methyl 26 18.482 0.29 LEDENOXID-(II) $$ 27 18.552 0.52 DIEPI-ALPHA-CEDREN I $$ 28 18.791 0.96 (-)-2,3,6,7-TETRAMETHYL-1,4,4a alpha,5,8,8A.beta,9.beta,9a.alpha
29 18.882 1.34 Farnesol $$ 2,6,10-Dodecatrien-1-ol,3,7,11-trimethyl- (CAS) 30 19.198 1.14 HEPTANE, 6-METHYL-2-P-TOLYL-$$
31 19.534 2.53 Spathulenol $$
32 19.624 0.95 5,8,11,14-Eicosatetranaeoic acid, ethyl ester, (all-Z)-(CAS) Ethyl 33 19.836 1.07 Bicyclo (3.1.0) hexan-3-ol,4-methylene-1(1-methylethyl) 34 20.139 15.30 BETA TUMERONE $$
35 20.397 23.36 AR-TUMERONE $$
36 21.056 17.92 Alpha longipinene $$ Tricyclo (5.4.0.0(2,8)undec-9-ene,2,6,6,9 37 21.872 2.04 2,6,10- Trimetlundecan-(5E)-2,5,9-trien-4-one $$
38 22.046 0.60 ALPHA SINENSIAL $$ 2,6,10-TRIMETHYL, 2,6,9,11-DODECAT 39 22.145 2.18 Trans carveol $$ 2-Cyclohexen-1-ol,2-methyl-5(1-methylethenyl) 40 22.323 3.31 Propanedinitrile, dicyclohexenyl- (CAS) DICYCLOHEXYLMALS 41 22.534 0.84 Alpha atlantone $$
42 22.832 1.62 EXOBORNYL ACETATE $$ Bicyclo (2.2.1)heptan-2-ol,1,7,7 43 23.307 2.01 Teresantalol $$ Tricyclo (2.2.1)(2,6)heptane-3-methanol
44 23.938 0.51 2-Butenoic acid, 2-methyl,2-(acetyloxy)-1,1a,2,3,4,6,7,10,11,11a 45 24.474 0.70
6-Methyl-6-(3’-isopropenyl-2’-methyl-cycloprop-1’en-1’-yl)-2heptane
46 24.558 0.76 2-Butenoic acid, 2-methyl,2(acetyloxy)1,1a, 2,3,4,6,7,10,11,11a 47 25.043 0.55 Propanedinitrile, dicyclohexenyl- (CAS) DICYCLOHEXYLMALS 48 25.254 0.74 1,7,7 Trimethlbicyclo(2.2.1)hept-2-yl-3-methyl-2-butenoate $$ 49 25.468 0.50 3,4-DIISOPROPYL-2,5-DIMETHYL-3-HEXENE $$
50 26.578 1.07 3-HEXENE,2,5-DIMETHYL-3,4-BIS (1-METHYLETHYL)- $$ 51 26.707 0.55 Ascaridole $$ 2,3-Dioxabicyclo (2.2.00)oct-5-ene,1-methyl
