69 LAMPIRAN A
HASIL UJI MUTU FISIK MASSA TABLET
Mutu fisk Formula Tablet Likuisolid Piroksikam yang diuji Replikasi F A F B F C F D
Persyaratan
Sudut
Diam I 34,7 23,17 27,15 26,06
30-40= cukup baik
(derajat) II 31,32 24,1 25,25 26,23 (Banker &
III 31,57 22,74 25,89 26,32 Anderson,
Rata-rata 32,53 23,34 26,10 26,20 1986)
SD 1,88 0,69 0,96 0,13
Hausner I 1,23 1,176 1,2 1,19 < 1,25
Ratio II 1,25 1,156 1,19 1,2 (Shervington
III 1,23 1,2 1,2 1,19 &
Rata-rata 1,24 1,18 1,2 1,19 Shervington,
SD 0,01 0,02 0,005 0,005 1998).
Carr’s I 18,99 15 16 15 18-21=
Index II 19,99 13,5 15 16 Cukup baik
(%) III 19 17 16 15 (Wells,
Rata-rata 19,32 15,16 15,66 15,33 1988)
70
LAMPIRAN B
HASIL UJI DISTRIBUSI UKURAN PARTIKEL
σg Replikasi
Formula A Formula B Formula C Formula D
1 1,93 1,92 1,90 1,92
2 1,96 1,91 1,90 1,92
3 1,93 1,95 1,91 1,89
Rata-rata ±SD 1,94±0,013 1,93±0,02 1,90 ±0,007 1,91 ±0,02
dg Replikasi
Formula A Formula B Formula C Formula D
1 102,69 136,88 109,54 97,76
2 99,4 135,99 107,55 94,22
3 99,04 130,79 106,06 93,25
71
LAMPIRAN C
HASIL UJI KEKERASAN TABLET LIKUISOLID PIROKSIKAM
REPLIKASI I
Kekerasan Tablet Likuisolid Piroksikam (Kp) No
Formula I Formula II Formula III Formula IV
1 16,3 11,45 16,3 16,3
Kekerasan Tablet Likuisolid Piroksikam (Kp) No
Formula I Formula II Formula III Formula IV
1 16,3 7,43 11,58 15,54
REPLIKASI III
Kekerasan Tablet Likuisolid Piroksikam (Kp) No
Formula I Formula II Formula III Formula IV
72
LAMPIRAN D
HASIL UJI KERAPUHAN TABLET LIKUISOLID PIROKSIKAM
Berat awal
Berat
akhir Kerapuhan SDrel
Formula Replikasi
73
LAMPIRAN E
HASIL UJI WAKTU HANCUR TABLET LIKUISOLID PIROKSIKAM
Waktu Hancur (menit) Replikasi
Formula A Formula B Formula C Formula D
I 2 2 3 2
II 1 2 2 3
III 1 2 2 3
74
LAMPIRAN F
HASIL UJI KERAGAMAN BOBOT TABLET LIKUISOLID PIROKSIKAM
Hasil Uji Keragaman Bobot Tablet Formula A
Replikasi I Replikasi II Replikasi III
No Bobot
Hasil Uji Keragaman Bobot Tablet Formula B
Replikasi I Replikasi II Replikasi III
75
Hasil Uji Keragaman Bobot Tablet Formula C Replikasi I Replikasi II Replikasi III
No Bobot
Hasil Uji Keragaman Bobot Tablet Formula D
Replikasi I Replikasi II Replikasi III
76
LAMPIRAN G
HASIL UJI KESERAGAMAN KANDUNGAN TABLET LIKUISOLID PIROKSIKAM
Hasil Uji Keragaman Kandungan Tablet Formula A Replikasi I Abs C sampel W sampel C teoritis Kadar (%)
Hasil Uji Keragaman Kandungan Tablet Formula A Replikasi II
77
Hasil Uji Keragaman Kandungan Tablet Formula A Replikasi III Abs C sampel W sampel C teoritis Kadar (%)
78
Hasil Uji Keragaman Kandungan Tablet Formula B Replikasi II Abs C sampel W sampel C teoritis Kadar (%)
79
Hasil Uji Keragaman Kandungan Tablet Formula C Replikasi I Abs C sampel W sampel C teoritis Kadar (%)
80
Hasil Uji Keragaman Kandungan Tablet Formula C Replikasi III Abs C sampel W sampel C teoritis Kadar (%)
81
Hasil Uji Keragaman Kandungan Tablet Formula D Replikasi II Abs C sampel W sampel C teoritis Kadar (%)
82
LAMPIRAN H
HASIL PENETAPAN KADAR TABLET LIKUISOLID PIROKSIKAM
Replikasi I Formula Replikasi Absorbansi Csampel
83
Replikasi II Formula Replikasi Absorbansi Csampel
(µg/ml)
Cteoritis (µg/ml)
Kadar (%)
Rata-rata ±SD
SD rel (%)
I 0,458 5,62 5,97 94,06 94,41
A II 0,454 5,57 5,91 94,18 ± 0,53
III 0,461 5,66 5,96 94,98 0,50
I 0,455 5,58 5,92 94,30 94,74
B II 0,463 5,68 5,92 95,96 ± 1,13
III 0,458 5,62 5,98 93,97 1,07
I 0,474 5,82 5,98 97,40 96,71
C II 0,468 5,75 5,97 96,21 ± 0,64
III 0,467 5,73 5,94 96,51 0,62
I 0,485 5,97 5,98 99,73 98,93
D II 0,481 5,91 5,98 98,90 ± 0,79
84
Replikasi III Formula Replikasi Absorbansi Csampel
(µg/ml)
Cteoritis (µg/ml)
Kadar (%)
Rata-rata±SD
SD rel (%)
I 0,465 5,71 5,94 96,14 95,28
A II 0,465 5,71 6,01 94,95 ± 0,79
III 0,463 5,68 6,00 94,75 0,75
I 0,461 5,66 5,92 95,53 95,93
B II 0,459 5,63 5,91 95,24 ± 1,00
III 0,465 5,71 5,88 97,02 0,96
I 0,467 5,73 5,97 95,99 97,89
C II 0,473 5,81 5,95 97,70 ± 2,04
III 0,481 5,91 5,92 99,97 2,00
I 0,492 6,06 6,00 100,87 99,40
D II 0,484 5,95 6,05 98,41 ± 1,31
85
LAMPIRAN I
HASIL UJI DISOLUSI TABLET LIKUISOLID PIROKSIKAM FORMULA A
Replikasi t A Csampel Csesungguhnya Wt %obat terlarut AUC
A 5 0,180 2,06 2,06 1,85 9,54 3,645
10 0,371 4,50 4,50 4,05 20,91 14,75
20 0,626 7,77 7,77 7,00 36,09 55,25
30 0,248 2,93 11,71 10,54 54,36 87,7
45 0,265 3,14 12,58 11,32 58,41 163,95
60 0,310 3,72 14,89 13,40 69,13 185,4
510,695
B 5 0,230 2,70 2,70 2,43 12,85 4,275
10 0,263 3,12 3,12 2,81 14,87 13,1
20 0,745 9,30 9,30 8,37 44,32 55,9
30 0,301 3,61 12,02 10,82 57,29 95,95
45 0,328 3,95 15,81 14,23 75,36 187,875
60 0,354 4,29 17,14 15,43 81,71 222,45
86
C 5 0,241 2,84 2,84 2,55 13,40 4,425
10 0,298 3,57 3,57 3,21 16,85 14,4
20 0,673 8,38 8,38 7,54 39,56 53,75
30 0,257 3,04 12,17 10,95 57,47 92,45
45 0,295 3,53 14,12 12,71 66,68 177,45
60 0,343 4,14 16,58 14,92 78,30 207,225
87
HASIL UJI DISOLUSI TABLET LIKUISOLID PIROKSIKAM FORMULA B
Replikasi t A Csampel Csesungguhnya Wt %obat terlarut AUC
A 5 0,264 3,13 3,13 2,82 14,77 5,31
10 0,481 5,91 5,91 5,32 27,88 20,35
20 0,690 8,59 8,59 7,73 40,51 65,25
30 0,293 3,50 14,02 12,61 66,08 101,7
45 0,328 3,95 15,81 14,23 74,54 201,3
60 0,364 4,41 17,66 15,89 83,24 225,9
619,81
B 5 0,255 3,02 3,02 2,72 14,33 5,52
10 0,470 5,77 5,77 5,20 27,42 19,8
20 0,739 9,22 9,22 8,30 43,80 67,5
30 0,290 3,47 13,86 12,48 65,84 103,9
45 0,337 4,07 16,27 14,64 77,29 203,4
60 0,359 4,35 17,40 15,66 82,65 227,25
88
C 5 0,249 2,94 2,94 2,65 13,79 4,935
10 0,468 5,75 5,75 5,17 26,96 19,55
20 0,686 8,54 8,54 7,69 40,07 64,3
30 0,283 3,38 13,50 12,15 63,34 99,2
45 0,329 3,97 15,86 14,28 74,41 198,225
60 0,357 4,32 17,30 15,57 81,14 223,875
89
HASIL UJI DISOLUSI TABLET LIKUISOLID PIROKSIKAM FORMULA C
Replikasi t A Csampel Csesungguhnya Wt %obat terlarut AUC
A 5 0,655 8,14 8,14 7,33 37,24 16,5
10 0,698 8,70 8,70 7,83 39,76 42,1
20 0,342 4,13 16,53 14,88 75,56 126,15
30 0,368 4,47 17,86 16,08 81,66 171,95
45 0,389 4,73 18,94 17,04 86,58 276
60 0,420 5,13 20,53 18,48 93,85 296,025
928,73
B 5 0,674 8,39 8,39 7,55 39,03 16,305
10 0,718 8,95 8,95 8,06 41,66 43,35
20 0,318 3,82 15,30 13,77 71,18 121,25
30 0,342 4,13 16,53 14,88 76,91 159,15
45 0,391 4,76 19,04 17,14 88,60 266,775
60 0,399 4,86 19,45 17,51 90,51 288,675
90
5 0,656 8,16 8,16 0,656 37,50 16,11
C 10 0,715 8,91 8,91 0,715 40,98 42,675
20 0,324 3,90 15,61 0,324 71,74 122,6
30 0,364 4,41 17,66 0,364 81,17 166,35
45 0,398 4,85 19,40 0,398 89,18 277,95
60 0,404 4,93 19,71 0,404 90,60 293,325
91
HASIL UJI DISOLUSI TABLET LIKUISOLID PIROKSIKAM FORMULA D
Replikasi t A Csampel Csesungguhnya Wt %obat terlarut AUC
A 5 0,668 8,31 8,31 7,48 37,88 15,915
10 0,294 3,52 14,07 12,66 64,11 50,35
20 0,370 4,49 17,96 16,17 81,87 144,15
30 0,401 4,89 19,55 17,60 89,12 168,85
45 0,427 5,22 20,89 18,80 95,19 273
60 0,443 5,43 21,71 19,54 98,93 287,55
939,82
B 5 0,681 8,48 8,48 7,63 38,56 16,11
10 0,298 3,57 14,27 12,84 64,92 51,175
20 0,370 4,49 17,96 16,17 81,71 145,05
30 0,393 4,79 19,14 17,23 87,08 167
45 0,431 5,27 21,09 18,98 95,94 271,575
60 0,439 5,38 21,50 19,35 97,81 287,475
92
C 5 0,678 8,44 8,44 7,60 38,21 16,155
10 0,303 3,63 14,53 13,08 65,77 51,7
20 0,368 4,47 17,86 16,08 80,86 145,8
30 0,403 4,91 19,66 17,69 88,99 168,85
45 0,435 5,32 21,30 19,17 96,42 276,45
60 0,450 5,52 22,07 19,86 99,90 292,725
93 LAMPIRAN J
Contoh Perhitungan
Contoh perhitungan sudut diam:
Formula A:
W persegi panjang = 1,8119 gram W lingkaran = 0,556 gram Luas persegi panjang = 360,96 cm2
94
Contoh perhitungan indeks kompresibilitas:
Formula A :
Contoh perhitungan akurasi & presisi:
Absorbansi = 0,459 → y = 0,078x + 0,0197 Konsentrasi sebenarnya = 5,63 ppm Konsentrasi teoritis = 6,03 ppm
= 93,40% Contoh perhitungan % obat terlepas:
% obat terlepas = 100%
Contoh perhitungan AUC pada disolusi:
Rumus:
(
)
Luas □ = 60 x penetapan kadar x dosis = 60 x 96,91% x 20 mg = 1162,92
97 LAMPIRAN K
LAMPIRAN L
LAMPIRAN M
Tabel Uji r
LAMPIRAN N
105
LAMPIRAN 0
HASIL UJI STATISTIK KEKERASAN TABLET ANTAR FORMULA
Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Column 1 3 48,41 16,13667 0,080033 Column 2 3 29,68 9,893333 4,774433 Column 3 3 39,14 13,04667 7,940633
Column 4 3 44,13 14,71 3,3481
ANOVA Source of
Variation SS df MS F P-value F crit
Between Groups 64,85487 3 21,61829 5,35663 0,025713 4,066181 Within Groups 32,2864 8 4,0358
Total 97,14127 11 Keterangan:
106
Hasil Uji HSD
HSD = 4,058039
FA FB FC FD
Mean 16,13667 9,893333 13,04667 14,71
FA 16,13667 0 -6,24333 -3,09 * -1,42667 *
FB 9,893333 0 3,153333 * 4,816667 *
FC 13,04667 0 1,663333 *
FD 14,71 0
107
LAMPIRAN P
HASIL UJI STATISTIK WAKTU HANCUR ANTAR FORMULA
Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Column 1 3 4 1,333333 0,333333
Column 2 3 6 2 0
Column 3 3 7 2,333333 0,333333
Column 4 3 8 2,666667 0,333333
ANOVA Source of
Variation SS df MS F P-value F crit
Between Groups 2,916667 3 0,972222 3,888889 0,055278 4,066181
Within Groups 2 8 0,25
Total 4,916667 11
Keterangan:
108
LAMPIRAN Q
HASIL UJI STATISTIK KERAPUHAN ANTAR FORMULA Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Column 1 3 1,535 0,511667 0,762608
Column 2 3 0,09 0,03 0,0001
Column 3 3 0,075 0,025 0,000175
Column 4 3 0,07 0,023333 3,33E-05
ANOVA Source of
Variation SS df MS F P-value F crit
Between Groups 0,530542 3 0,176847 0,927216 0,470709 4,066181 Within Groups 1,525833 8 0,190729
Total 2,056375 11
Keterangan:
109
LAMPIRAN R
HASIL UJI STATISTIK DISTRIBUSI UKURAN PARTIKEL (dg 50%) ANTAR FORMULA
Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Column 1 3 301,13 100,3767 4,046033 Column 2 3 403,66 134,5533 10,82003 Column 3 3 323,15 107,7167 3,048433 Column 4 3 285,23 95,07667 5,635433
ANOVA Source of
Variation SS df MS F P-value F crit
Between Groups 2766,295 3 922,0984 156,6201 1,92E-07 4,066181 Within Groups 47,09987 8 5,887483
Total 2813,395 11
Keterangan:
110
Hasil Uji HSD
HSD = 4,90135
FA FB FC FD
Mean 100,3767 134,5533 107,7167 95,07667
FA 100,376 0 34,17667 7,34 * -5,3 *
FB 134,553 0 -26,8367 * -39,4767 *
FC 107,716 0 -12,64 *
FD 95,0766 0
111
LAMPIRAN S
HASIL UJI STATISTIK PENETAPAN KADAR TABLET LIKUISOLID PIROKSIKAM ANTAR FORMULA
Keterangan:
Fhitung >Ftabel (3,8)= 4,07 sehingga H ditolak dan ada perbedaan yang bermakna antar formula. Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Column 1 3 286,6 95,53333 1,6106333 Column 2 3 286,12 95,37333 0,3584333 Column 3 3 293,03 97,67667 0,7737333 Column 4 3 297,07 99,02333 0,1154333
ANOVA Source of
Variation SS df MS F P-value F crit
Between Groups 27,9307 3 9,310233 13,029354 0,001906 4,066181 Within Groups 5,716467 8 0,714558
112
Hasil Uji HSD
HSD = 1,70753
FA FB FC FD
Mean 95,53333 95,37333 97,67667 99,02333
FA 95,5333 0 -0,16 2,143333 * 3,49 *
FB 95,3733 0 2,303333 * 3,65 *
FC 97,6766 0 1,346667 *
FD 99,0233 0
113
LAMPIRAN T
HASIL UJI STATISTIK DISOLUSI BERDASARKAN %ED60 TABLET LIKUISOLID PIROKSIKAM
ANTAR FORMULA
Keterangan:
Fhitung > Ftabel (3,8)= 4,07 sehingga H ditolak dan ada perbedaan yang bermakna antarformula. Anova: Single Factor
SUMMARY
Groups Count Sum Average Variance
Column 1 3 143,2698 47,75661 13,15506 Column 2 3 162,4968 54,16559 1,226545 Column 3 3 234,7027 78,23424 0,59332 Column 4 3 238,9391 79,64637 0,141782
ANOVA Source of
Variation SS df MS F P-value F crit
Between Groups 2413,111 3 804,3705 212,8427 5,74E-08 4,066181 Within Groups 30,23342 8 3,779178
114
Hasil Uji HSD
HSD = 3,92690
FA FB FC FD
Mean 47,75661 54,16559 78,23424 79,64637
FA 47,7566 0 6,408977 30,47763 * 31,88976 *
FB 54,1655 0 24,06865 * 25,48078 *
FC 78,2342 0 1,412131 *
FD 79,6463 0
115 LAMPIRAN U
UJI F KURVA BAKU PENETAPAN KADAR
Uji Kesamaan Regresi (HCl)
S X2 SXY S Y2 N SSi RDF
Regresi I 234,3053 18,7803 1,5110 5 1,4309 4 Regresi II 237,9520 19,1818 1,5475 5 1,4669 4 Regresi III 231,5887 19,4878 1,6424 5 1,5583 4
703,8460 57,4499 4,7010 4,4561 Ssc = 4,619369117
