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EXPRESSION OF HOXA10 GENE IN ENDOMETRIAL TISSUE OF
INFERTILE WOMEN
Johan Ricardo, Henry Salim, M. Rusda, Letta Lintang, Edi Ardiansyah, Hanudse Hartono
Obstetric and Gynecology Department Faculty of Medicine Universitas Sumatera Utara
General Hospital H Adam Malik Medan
ABSTRACT
Objective : To find the difference of HOXA10 gene expression in endometrial tissue of infertile women with endometriosis and normal endometrium.
Method : This is an analytic study with cross sectional approach. 40 subjects who fulfilled the inclusion criteria were obtained, 20 subjects with endometriosis
and 20 subjects without endometriosis. Endometrial tissue from infertile women
with endometriosis and from normal women were obtained using endometrial
biopsy. Immunohistochemistry examination was then performed to evaluate
HOXA10 expression. This study was performed in Obstetric and Gynecology
Department of Adam Malik General Hospital Medan from March to April 2015.
The obtained data was then analyzed statistically.
Result : Majority of subjects from endometriosis group were from the age group of 31-40 years old with 13 subjects (65%). While the majority of subjects from
non endometriosis group were from the age group 41-50 years old with 15
subjects (75%). Based on parity, majority of the subject from endometriosis were
nulliparous with 19 subjects (95%). From the non endometriosis group, subjects
with parity of 1-3 were 11 subjects (55%). Based on HOXA10 expression
intensity, 14 subjects (70%) showed no expression, expression of +1 with 6
subjects (30%). While from non endometriosis group, expression of +3 were
found in 13 subjects (65%), and expression of +2 were found in 7 subjects (35%),
p<0,05.
Conclusion : There is a significant difference of HOXA10 gene expression in endometrial tissue of subject with endometriosis and without endometriosis.
Keywords : HOXA10, endometriosis, infertility
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EKSPRESI GEN HOXA 10 PADA JARINGAN ENDOMETRIUM
PENDERITA ENDOMETRIOSIS INFERTIL
Johan Ricardo, Henry Salim, M. Rusda, Letta Lintang, Edi Ardiansyah, Hanudse Hartono
Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Sumatera Utara
RSUP H Adam Malik Medan Abstrak
Tujuan : Untuk mengetahui perbedaan ekspresi gen HOXA10 pada jaringan endometrium wanita infertil dengan endometriosis dan endometrium normal.
Metode : Penelitian ini merupakan studi analitik dengan pendekatan potong lintang. 40 subjek yang memenuhi kriteria inklusi dikumpulkan, 20 subjek dengan
endometriosis dan 20 subjek tanpa endometriosis. Jaringan endometrium pada
wanita infertil dengan endometriosis dan pada wanita normal didapatkan dengan
biopsi endometrium. Pemeriksaan immunohistokimia dilakukan untuk
mengevaluasi ekspresi HOXA10. Penelitian ini dilakukan di Departemen Obstetri
dan Ginekologi RSUP HAM Medan dari bulan Maret sampai April 2015. Data
yang telah diperoleh kemudian dianalisa secara statistik.
Hasil : Sebagian besar subjek dari kelompok endometriosis berasal dari kelompok umur 31-40 tahun didapati sebanyak 13 subjek (65%). Dimana
sebagian besar subjek yang berasal dari kelompok non endometriosis umur
41-50 tahun didapati sebanyak 15 subjek (75%). Berdasarkan kriteria paritas,
sebagian besar subjek dengan endometriosis nullipara didapati sebanyak 19
subjek (95%). Dari kelompok non endometriosis, sebagian besar subjek dengan
paritas 1-3 didapati sebanyak 11 subjek (55%). Berdasarkan intensitas ekspresi
HOXA10, 14 subjek (70%) menunjukkan tidak ada ekspresi, ekspresi +1 didapati
6 subjek(30%). Kelompok non endometriosis dengan ekspresi +3 ditemukan
pada 16 subjek (65%), dan ekspresi +2 ditemukan pada 7 subjek (35%), p<0,05
Kesimpulan : Didapati perbedaan yang signifikan dari subjek dengan ekspresi gen HOXA10 pada jaringan endometrium dan tanpa endometriosis.
Kata kunci : HOXA10, endometriosis, infertil
