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BAB V KESIMPULAN DAN SARAN

5.2 Saran

Sebagai tindak lanjut dari hasil penelitian dan uji coba yang telah dilakukan, diajukan beberapa saran sebagai berikut:

1. Hasil penelitian ini diharapkan menjadi tambahan informasi mengenai ekstrak bunga telang (Clitoria ternatea) dalam menurunkan kadar kolesterol total dan berat badan pada kondisi obesitas.

2. Perlu adanya uji lengkap terhadap kandungan zat yang terdapat pada ekstrak bunga telang (Clitoria ternatea)

DAFTAR PUSTAKA

Al-Snafi, A. E. (2016). Pharmacological importance of Clitoria ternatea–A review. IOSR Journal of Pharmacy, 6(3), 68-83.

Arsianti, A., Mahindra, S. P., Azizah, N. N., Fajrin, A. M., & Nadapdap, L. D.

(2022). Phytochemical Analysis, Antioxidant and Anticancer Effects of Clitoria ternatae Extract on Breast T47D Cancer Cells. Indonesian Journal of Medical Chemistry and Bioinformatics, 1(1), 3.

Asadi, N., Bahmani, M., Kheradmand, A., & Rafieian-Kopaei, M. (2017). The Impact of Oxidative Stress on Testicular Function and the Role of Antioxidants in Improving it: A Review. Journal of clinical and diagnostic

research: JCDR, 11(5), IE01–IE05.

https://doi.org/10.7860/JCDR/2017/23927.9886

Aziza, V., Ulimaz, T. A., Ustari, D., Suganda, T., Concibido, V., Irawan, B., &

Karuniawan, A. 2021. Keragaman Fenotipik Bunga Telang Double Petal Asal Indonesia dan Thailand Berdasarkan Morfologi Bunga. Al-Kauniyah:

Jurnal Biologi, 14(1), pp. 78–89.

Bisht, S., Faiq, M., Tolahunase, M., & Dada, R. (2017). Oxidative stress and male infertility. Nature reviews. Urology, 14(8), 470–485.

https://doi.org/10.1038/nrurol.2017.69

Chaudhuri, G. R., Das, A., Kesh, S. B., Bhattacharya, K., Dutta, S., Sengupta, P.,

& Syamal, A. K. (2022). Obesity and male infertility: multifaceted reproductive disruption. Middle East Fertility Society Journal, 27(1), 8.

Chayaratanasin, P., Caobi, A., Suparpprom, C., Saenset, S., Pasukamonset, P., Suanpairintr, N., Barbieri, M. A., & Adisakwattana, S. (2019). Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression. Molecules (Basel, Switzerland), 24(10), 1894.

https://doi.org/10.3390/molecules24101894

Darbandi, M., Darbandi, S., Agarwal, A., Sengupta, P., Durairajanayagam, D., Henkel, R., & Sadeghi, M. R. (2018). Reactive oxygen species and male reproductive hormones. Reproductive Biology and Endocrinology, 16(1).

doi:10.1186/s12958-018-0406-2

Di Ciaula, A., Garruti, G., Baccetto, R. L., Molina-Molina, E., Bonfrate, L., Portincasa, Diamond AM (2021) Essential Nutrients in Human Body.

Vitam Miner Vol.10 No.6: 10.e161

Dutta, S., Sengupta, P., & Muhamad, S. (2019). Male reproductive hormones and semen quality. Asian Pacific Journal of Reproduction, 8(5).

El Salam M. A. A. (2018). Obesity, An Enemy of Male Fertility: A Mini Review.

Oman medical journal, 33(1), 3–6. https://doi.org/10.5001/omj.2018.02 Fietz, D., & Bergmann, M. (2017). Functional Anatomy and Histology of the

Testis. Endocrinology of the Testis and Male Reproduction, 313–341.

doi:10.1007/978-3-319-44441-3_9

Feingold K. R. (2022). Lipid and Lipoprotein Metabolism. Endocrinology and metabolism clinics of North America, 51(3), 437–458.

https://doi.org/10.1016/j.ecl.2022.02.008

Foster, J. R., & Frost, D. (2018). The History of the Rat. Boorman’s Pathology of the Rat, 7–12. doi:10.1016/b978-0-12-391448-4.00003-4

Goh, S. E., Kwong, P. J., Ng, C. L., Ng, W. J., & Ee, K. Y. (2021). Antioxidant- rich Clitoria ternatea L. flower and its benefits in improving murine reproductive performance. Food Science and Technology, 42, e25921.

Grande, G., Barrachina, F., Soler-Ventura, A., Jodar, M., Mancini, F., Marana, R., ... & Milardi, D. (2022). The role of testosterone in spermatogenesis:

Lessons from proteome profiling of human spermatozoa in testosterone deficiency. Frontiers in Endocrinology, 13, 852661.

Hall, K. D., & Guo, J. (2017). Obesity Energetics: Body Weight Regulation and the Effects of Diet Composition. Gastroenterology, 152(7), 1718–1727.e3.

doi:10.1053/j.gastro.2017.01.052

Hawari, H., Pujiasmanto, B., & Triharyanto, E. (2022). Morfologi dan kandungan flavonoid total bunga telang di berbagai ketinggian tempat tumbuh berbeda. Kultivasi, 21(1), 88-96.

Huff T, Boyd B, Jialal I. Physiology, Cholesterol. (2022) In: StatPearls. StatPearls Publishing, Treasure Island (FL); PMID: 29262185.

Johnsen S. G. (1970). Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335

hypogonadal males. Hormones, 1(1), 2–25.

https://doi.org/10.1159/000178170

Kim, D. (2020). Predictors of obesity. Obesity, 191–194. doi:10.1016/b978-0-12- 818839-2.00015-6

Khutami, C., Sumiwi, S. A., Khairul Ikram, N. K., & Muchtaridi, M. (2022). The effects of antioxidants from natural products on obesity, dyslipidemia, diabetes and their molecular signaling mechanism. International Journal of Molecular Sciences, 23(4), 2056.

Lee, Sang-Il, et al. (2011). Anti-obesity Effect of Monascus pilosus Mycelial Extract in High Fat Diet-induced Obese Rats. J. Appl. Biol. Chem. 54(3), 197-205

Lourenço, S. C., Moldão-Martins, M., & Alves, V. D. (2019). Antioxidants of natural plant origins: From sources to food industry applications. Molecules, 24(22), 4132.

Magfira (2018) Analisis Penghambatan Ekstrak Etanol Batang Kembang Bulan (Tithonia ediversifolia) Terhadap Reaksi Oksidasi dari Radikal Bebas Dengan Metode DPPH ABTS dan FRAP‟, Universitas Hasanuddin Makasar.

Mahmudiono, T., Abihail, C. T., Priambudi, D. A., Elkarima, E., Megatsari, H., Indriani, D., & Yoga, G. P. (2022). The Effect of Online Order Development on Fast Food, Vegetable, and Fruit Consumption Behavior on Students in Surabaya. Open Access Macedonian Journal of Medical Sciences, 10(E), 630-637.

Marpaung, A. M. (2020). Tinjauan manfaat bunga telang (clitoria ternatea l.) bagi kesehatan manusia. Journal of Functional Food and Nutraceutical, 63-85.

McMurray, F., Patten, D. A., & Harper, M.-E. (2016). Reactive Oxygen Species and Oxidative Stress in Obesity-Recent Findings and Empirical Approaches. Obesity, 24(11), 2301–2310. doi:10.1002/oby.21654

O'Donnell, L., Stanton, P., & de Kretser, D. M. (2015). Endocrinology of the male reproductive system and spermatogenesis.

Phrueksanan, W., Yibchok-anun, S., & Adisakwattana, S. (2014). Protection of Clitoria ternatea flower petal extract against free radical-induced hemolysis and oxidative damage in canine erythrocytes. Research in

veterinary science, 97(2), 357–363.

https://doi.org/10.1016/j.rvsc.2014.08.010

Purwanto, U. M. S., & Aprilia, K. (2022). Antioxidant Activity of Telang (Clitoria ternatea L.) Extract in Inhibiting Lipid Peroxidation. Current Biochemistry, 9(1), 26-37.

Romieu, I., Dossus, L., Barquera, S., Blottière, H. M., Franks, P. W., Gunter, M., Hwalla, N., Hursting, S. D., Leitzmann, M., Margetts, B., Nishida, C., Potischman, N., Seidell, J., Stepien, M., Wang, Y., Westerterp, K., Winichagoon, P., Wiseman, M., Willett, W. C., & IARC working group on Energy Balance and Obesity (2017). Energy balance and obesity: what are the main drivers?. Cancer causes & control : CCC, 28(3), 247–258.

https://doi.org/10.1007/s10552-017-0869-z

Rubino, F., Puhl, R. M., Cummings, D. E., Eckel, R. H., Ryan, D. H., Mechanick, J. I., Nadglowski, J., Ramos Salas, X., Schauer, P. R., Twenefour, D., Apovian, C. M., Aronne, L. J., Batterham, R. L., Berthoud, H. R., Boza,

C., Busetto, L., Dicker, D., De Groot, M., Eisenberg, D., Flint, S. W., … Dixon, J. B. (2020). Joint international consensus statement for ending stigma of obesity. Nature medicine, 26(4), 485–497.

https://doi.org/10.1038/s41591-020-0803-x

Silber, S. (2018). Testis Development, Embryology, and Anatomy. Fundamentals of Male Infertility, 3–12. doi:10.1007/978-3-319-76523-5_1

Suarna, I. W., & Wijaya, I. M. S. (2021). Butterfly pea (Clitoria ternatea L.:

Fabaceae) and its morphological variations in Bali. Journal of Tropical Biodiversity and Biotechnology, 6(2), 63013.

Suede, S. H., Malik, A., & Sapra, A. (2020). Histology, spermatogenesis.

Smith, J.B., dan Mangkoewidjojo, S., 1988, Pemeliharaan Pembiakan dan Penggunaan Hewan Percobaan di Daerah Tropis, Penerbit Universitas Indonesia, Jakarta.

Wang, E. Y., Huang, Y., Du, Q. Y., Yao, G. D., & Sun, Y. P. (2017). Body mass index effects sperm quality: a retrospective study in Northern China. Asian journal of andrology, 19(2), 234–237. https://doi.org/10.4103/1008- 682X.169996

Witosari, N., & Widyastuti, N. (2014). Pengaruh pemberian jus daun ubi jalar (Ipomoea batatas (l.) lam) terhadap kadar kolesterol total tikus wistar jantan (Rattus norvegicus) yang diberi pakan tinggi lemak (Doctoral dissertation, Diponegoro University).

Wolfenshon., and Lloyd. 2013. Handbook of Laboratory Animal Management and Welfare, 4th ed., Wiley-Blackwell : West Sussex, 234.

LAMPIRAN Lampiran 3: Master Data

Uji Normalitas

Tests of Normality

Kelompok

Kolmogorov-Smirnova Shapiro-Wilk

Statistic df Sig. Statistic df Sig.

Hasil Kontrol .172 6 .200* .957 6 .798

P1 .199 6 .200* .935 6 .621

P2 .164 6 .200* .972 6 .906

P3 .161 6 .200* .955 6 .779

*. This is a lower bound of the true significance.

a. Lilliefors Significance Correction

Uji Homogenitas

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.

Hasil Based on Mean .676 3 20 .577

Based on Median .620 3 20 .610

Based on Median and with adjusted df

.620 3 18.274 .611

Based on trimmed mean .675 3 20 .577

Uji One Way Anova

ANOVA

Hasil

Sum of Squares df Mean Square F Sig.

Between Groups 1403.385 3 467.795 330.889 .000

Within Groups 28.275 20 1.414

Total 1431.660 23

Uji Post Hoc LSD

Multiple Comparisons

Dependent Variable: Hasil LSD

(I) Kelompok (J) Kelompok

Mean Difference (I-

J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

Kontrol P1 -19.31667* .68648 .000 -20.7486 -17.8847

P2 -6.96667* .68648 .000 -8.3986 -5.5347

P3 -1.23333 .68648 .088 -2.6653 .1986

P1 Kontrol 19.31667* .68648 .000 17.8847 20.7486

P2 12.35000* .68648 .000 10.9180 13.7820

P3 18.08333* .68648 .000 16.6514 19.5153

P2 Kontrol 6.96667* .68648 .000 5.5347 8.3986

P1 -12.35000* .68648 .000 -13.7820 -10.9180

P3 5.73333* .68648 .000 4.3014 7.1653

P3 Kontrol 1.23333 .68648 .088 -.1986 2.6653

P1 -18.08333* .68648 .000 -19.5153 -16.6514

P2 -5.73333* .68648 .000 -7.1653 -4.3014

*. The mean difference is significant at the 0.05 level.

Lampiran 4: Dokumentasi Penelitian Pembuatan Ekstrak Bunga Telang

Uji Fitokimia Ekstrak Bunga Telang

No. Senyawa Ekstrak Bunga Telang (Clitoria

ternatea)

1. Flavonoid

2. Saponin

3. Tannin

4. Alkaloid

5. Triterpenoid

Prosedur Perlakuan

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