ATOM INDONESIA JOURNAL
Referee’s Report
Article No. : # 403
Title of Paper : PROFILE OF MIBI LIQUID PHASE RADIOPHARMACEUTICAL FOR MYOCARDIAL IMAGING
Referee Name :
Comment on Descriptions 1. Title
[X ] Appropriate [ ] Should be changed
2. Abstract
Yes[ X ] No[ ] Is the length reasonable?
Yes[ ] No[ ] Is it an appropriate summary of the content?
3. Main Text
Yes[ ] No[ ] Is there anything new in this work?
Yes[ ] No[ X ] Is the relation to previous studies adequately stated?
Yes[ ] No[ X ] Are the assumption(s) and/or method(s) described comprehensively?
Yes[ ] No[ ] Are the new results adequately emphasized?
Line # Referee’s Comments
15-19
Radiopharmaceutical is a diagnostic kit todetect the interference, obstruction and the existence of infections and tumors in the organs using a compound that has been labeled with radioactive material as a tracer [1].
This sentence shall be corrected. It is not enough to define radiopharmaceutical as only a diagnostic kit, because a radiopharmaceutical can also be a therapeutic agent. It depends on the kind of the radionuclide used as labeling agent.
47-49
MIBI radiopharmaceutical was formulated indry form by lyophilization process using a Freeze Dryer, that has a longer shelf life than liquid form.
It is better to put relevant reference number(s) at the end of the sentence.
Hypothesis or objectives being used as reason(s) to carry out the presented study should be clearly written in the INTRODUCTION part. The above cited sentence is not enough to illustrate the present status and condition of the present preparation and application of the MIBI-dry kit used so far.
77-78 Radiochemical purity determination of MIBI liquid phase It is better to put reference number(s) in this part of procedure step.
91-97
Please give attention that the presented narration is more related to the stabilization of the labeled liquid kit than that of the liquid kit itself as mentioned in the article title. The stabilization of the liquid kit shall be studied on several samplings of liquid kit batch(es) which are labeled in different day respectively.
MIBI Liquid Kit Formulation 1 and Formulation 2 were tested stability by storage kit in the freezer. At a certain time period MIBI liquid kit were labeled with technetium-99m.
Here it is. This is the stability test of the liquid kit stored in freezer. Pleased compare the structure of this sentence to that of the sentence in line 91 – 97.But you still should revise the sentence of line 111 – 114 (were tested stability by storage kit in the freezer or were stability tested based on the storage system??)
Fig 2 and fig 3
Please consider to compare the stabilization profiles of liquid MIBI kit formulation 1 showed in Fig 2 and Fig 3. The profiles presented in Fig 2 and Fig 3 are differ
significantly one to another, indicating that the precision of the labeling result is low [?].
152-153
The chemical reaction should be re-written more clear in agree with the narration in lines 160 -165 below. How can you overview to distinguish 99mTc-citratefrom 99mTc- MIBI?
160-165
Initially, 99mTc-citrate is formed complexes with Tc(V) and Tc(IV) with Sn(II) and Sn(IV). When heating process, coper-MIBI complex is broken andreleasing MIBI ligand with an isonitrile group that forms a complex with 99mTc-citrate to form 99mTc- MIBI [8].
Please explain clearly the sentence stating that 99mTc-citrate is formed complexes with Tc(V) and Tc(IV) with Sn(II) and Sn(IV).
173-176 The narration regarding Fig 4 needs more scientific meaning to explain the presented data.
Fig 6 Please consider to enlarge this figure. The presented figure cannot be read.
229-231
Formulation 2 of MIBI liquid kit has a highstability than formulation 1 which consist of twovial.
The sentence should be revised. Which formulation consisting of two vials ?
LIST OF REFERENCES. Please use more primary and newer references meeting the guide for writing scientific paper from LIPI.
Final comments and recommendations:
1. THE CONTENTS OF THIS ARTICLE MEETS THE MISSION OF ATOM
INDONESIA JOURNAL, BUT REVISION SHOULD BE PERFORMED. ADVISES AND SUGGESTION GIVEN ABOVE SHOULD BE CONSIDERED.
2. DRY KIT FORMULATION IS GENERALLY PREFERRED AS COMPARED TO LIQUID KIT ESPECIALLY IN REGARD TO THE STABILIZATION. SO THE FIRST QUESTION IS : WHY DID THE AUTHORS STUDIED THE LIQUID KIT PREPARATION EVEN THOUGH THEIR INSTITUTION HAS WELL
DEVELOPED THE DRYKIT FORM OF
99mTc-MIBI ??
This paper is recommended to be [ ] Accepted without further revision [ ] Accepted with minor revision [ X ] Major Revision is required [ ] Rejected
