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Title: Evaluation of locomotor function and microscopic structure of the spinal cord in a mouse model of experimental autoimmune encephalomyelitis following treatment with syngeneic mesenchymal stem cells
Author(s): Mitra, NK (Mitra, Nilesh Kumar); Bindal, U (Bindal, Umesh); Hwa, WE (Hwa, Wong Eng); Chua, CLL (Chua, Caroline L. L.); Tan, CY (Tan, Chek Ying) Source: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY Volume: 8 Issue: 10 Pages: 12041-12052 Published: 2015 Times Cited in Web of Science Core Collection: 3
Total Times Cited: 3
Usage Count (Last 180 days): 0 Usage Count (Since 2013): 1 Cited Reference Count: 27
Abstract: Out of the minor myelin proteins, most significant one is myelin oligodendrocyte glycoprotein (MOG). Mesenchymal stem cells (MSCs) have proven
immunoregulatory capacity. The objective of this study was to investigate the effects of syngeneic MSCs on mouse model of experimental autoimmune encephalomyelitis (EAE) through observation of locomotion by footprint analysis, histological analysis of spinal cord and estimation IL-17. C57BL/6 mice (10 weeks, n = 16) were immunized with 300 mu g of MOG(35-55) and 200 mu L of complete Freund's adjuvant (CFA) to produce EAE model. Sham-treated control (n = 8) were injected with CFA. Half of immunized mice were given 100 mu L of PBS (n = 8) and next half (n = 8) received 1 x 10(5) MSCs on day 11 through the tail veins. Clinical scoring showed development of EAE (loss of tonicity of tail and weakness of hind limb) on day 10. Following MSC treatment, clinical scores and hindlimb stride length showed significant improvement on day 15 onwards, compared to day 10 (P < 0.05). Under LFB staining, while PBS-treated group of EAE mice showed pale and degenerated axons in anterolateral white column of lumbar spinal cord, MSC-treated group showed numerous normal-looking axons. H&E staining showed normal axons in anterolateral white column and reduction of macrophages in MSC-treated EAE mice group. A lower level of IL-17 was observed in MSC treated EAE mice, compared to PBS-treated EAE mice. Our results suggest that Intravenous MSC has the potential to improve the locomotion and regeneration of axons in spinal cord in MOG-induced EAE model.
Accession Number: WOS:000367814200009 PubMed ID: 26722389
Language: English Document Type: Article
Author Keywords: Experimental autoimmune encephalomyelitis; mesenchymal stem cells; footprint analysis; histology; spinal cord
KeyWords Plus: MULTIPLE-SCLEROSIS; ANIMAL-MODELS; INFLAMMATORY DEMYELINATION; TH17 CELLS; IN-VIVO; PATHOGENESIS; REACTIVITY;
THERAPY; SYSTEM; REPAIR
Addresses: [Mitra, Nilesh Kumar; Bindal, Umesh; Hwa, Wong Eng] Taylors Univ, Sch Med, Subang Jaya, Selangor, Malaysia.
[Chua, Caroline L. L.] Taylors Univ, Sch Biosci, Subang Jaya, Selangor, Malaysia.
[Tan, Chek Ying] Taylors Univ, Res Lab, Sch Med Pharm & Biosci, Subang Jaya, Selangor, Malaysia.
Reprint Address: Mitra, NK (reprint author), Taylors Univ, Sch Med, 1 Jalan Taylors, Subang Jaya, Selangor, Malaysia.
E-mail Addresses: [email protected] Author Identifiers:
Author ResearcherID Number ORCID Number
Mitra, Nilesh Kumar 0000-0002-8487-4607
Publisher: E-CENTURY PUBLISHING CORP
Publisher Address: 40 WHITE OAKS LN, MADISON, WI 53711 USA Web of Science Categories: Oncology; Pathology
Research Areas: Oncology; Pathology IDS Number: DA5AJ
ISSN: 1936-2625
29-char Source Abbrev.: INT J CLIN EXP PATHO ISO Source Abbrev.: Int. J. Clin. Exp. Pathol.
Source Item Page Count: 12 Funding:
Funding Agency Grant Number
Centre for Research and Development of Taylor's University, Malaysia
This work was supported by research grant from the Centre for Research and Development of Taylor's University, Malaysia granted to NKM. The authors acknowledge the support from the Brain Research Institute of Monash University Sunway Campus, Malaysia for use of the animal facility for the
maintenance of the animals.
Open Access: No
Output Date: 2017-11-15
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