KHOA HOC C 6 N G N G H £
[MGHIEIM CUU AIMH HUOIMG CUA CAC PHUQIMG PHAP THU PHAIM V A THOI DIEM CUU PHOI OEIM KHA IMAIMG TAD CDIM LAI GIUA CAC GIDIMG HDA LILY (CHI UUUM\
T r t n h Khdc Quang^
T6MTAT
Muc dich ciia nghidn ciru nay Irt so sanh anh hinnig cu.i phuong ph^p thy phdn thong thudng va phuong phap thu pliiin cdt voi nliuy (CSM) den MI tgo llianh cnn lai khac Inii F, va con lai lyi BC, ciia hoa lily.
Nghien cini n.iy dfl MI dung 12 to hay, Ini voi 6 nhdm lai Asiatic (A), LA (LA), Oriental (O), OT (OT). L fonnnlongi (F) va L loiigi/hrumThunb. (L). Hai phuong p h i p nuoi cdy \Al cdt bdu nhuy (OSC) va nuoi cay tlii phoi (ESR) dugc su dung dc nudi cdy c i c nn.ln d5 duoc thu Unh ho^c tiii ph6i sau khi thu phdn cdt voi nhuy va thu phdn tliOng thuonK Krt qua nghidn cuu chi ra rdng CSM In phuong phap thich h^p va hieu qua doi vrri the' h^ con lai F,. Ty 1$ dftu qua dai cao (30,0-65,0%). Phumig ph.ip thu phdn thong thuong thi thich hap h<m voi llic h$ lai lai BC, v<Vi ty 1$ d(iu qua d?l 20.0-87.f)! Doi v6i lo hop lai khac loai. sir dung phuong phap OSC o Ihrn gian 8-10 ngay sau Ihu phdn cho ty 10 noftn nay mdm cao nhdt (40.7-50,6%). Khi sir dung phiroiig phap ESR, Hirri gian thu nofln trit nhdt \A lu 45-60 ngay sau Ihu phdn (tiiy thu^c vao to h^p taO va ty le phoi nay mdm 6 cac to hgp lai khac nhau dao dong tii 8,1-21,3%.
Tir kh6a: 77ip pliHi thdng thutmg. thp phan cit voi nhpy, nudi cny tin phdi, nuoi ciy l;il cit bau nhuy. lai khac loai, loai hlium.
L flAT VAN BE
C h i Lilium b a o g o m k h o a n g 80 loai. c o n g u o n g o c b Bdc b a n c a u va d u p c p h a n loai t h d n h 7 n h o m ; t r o n g d o c o 3 n h d m lai q u a n t r p n g d o i vdi c o n g tdc c h p n tao g i o n g lily la n h o m lai O r i e n t a l ( 0 ) , n h o m lai Asiatic (A) va n h o m lai L o n g i f l o r u m (L). T u y n h i e n m p t t r o n g n h i m g k h o k h a n g a p p h a i k h i lai x a g i u a c a c loai t h u p c c h i Ulium la h i e n t u o n g b a t h p p t r u o c va s a u t h u tinh (Van T u y l & c s , 1991), d i ^ u n a y c o t h ^ k h d c p h u c d u p c b d n g vi$c sit d u n g cdc p h u o n g p h a p t h u p h d n (thu p h d n cdt voi n h u y , t h u p h a n in vitro) va ciiu phoi k h a c n h a u ( n u o i c a y n o a n , nuoi cay lat cdt b a u n h u y , n u o i c a y p h o i va tiii phoi) (Chi, 2002).
P h u o n g p h d p t h u p h a n cdt voi n h u y la p h u o n g p h a p d u p c sii d u n g p h d b i e n n h a t d^ v u p t q u a n h i r n g r a o c a n t r u o c t h y tinh b c h i Lilium. J u o z a s P r o s e v i f i u s va Lidija S t i i k u l y t e (2004) d a t h u d u p c c o n lai giiia n h o m Asiatic va L pumilum. k h i s u d u n g p h u o n g p h d p t h u p h a n cdt voi n h u y . Lim (2006) d a chi r a r d n g t h u p h a n cdt voi n h y y la tot h o n doi voi t h e h e lai F | va p h u o n g p h d p t h u p h a n t h o n g t h u o n g la t o t h o n d d i vdi q u d n t h e lai lai ( b a c k - c r o s s ) . N u o i cay lat cdt b a u n h u y va n u o i c a y tiii p h o i d a d u o c sir d u n g t h d n h c o n g d e tao r a c a c c o n lai k h d c loai 6 c h i
Vi?n Nghien ciru Rau qua
hlium. C a c lat cdt c o t h ^ d u p c n u o i c a y t h d n h conger giai d o a n 40 n g d y s a u t h u p h a n d o i voi to h p p lai giiia hlium longiflorum vd L xelegance (Kanoh &cs, 1988) h o a c Id t u 5-8 n g a y s a u t h u p h a n giiia gidng Gelria x Indian S u m m e r (Van T u y l 8L CS, 1991), tii 10- 11 n g a y s a u t h u p h a n giiia Ulium longiflorum 'Gerlia' X Asiatic h y b r i d ' M o n t b l a n c ' ( C h i , 2 0 0 2 ) . Doi voi nuoi cay tiii p h o i , t h o i g i a n tot n h a t la 38-70 n g a y sau thu p h a n tiiy tiiuoc to h o p lai ( C h i , 2 0 0 2 ) .
L VATUEU VA PHUONG PHAP NGHEN CUU 1. Vdt U§u n g h i d n ciiu
G 6 m 1 g i o n g h o a lily t h u o c n h o m lai Asiatic (P^, 1 g i o n g t h u o c n h o m lai LA (LA), 3 g i d n g t h u p c nhom lai O r i e n t a l ( 0 ) , 7 g i o n g t h u o c n h o m O T ( 0 1 ) , 1 g i d n g t h u p c n h o m lai L formolongi (F) va 1 gidng t h u p c loai L longiflorum T h u n b . (L) d u p c s u dung d^ lai tiio. Cdc c u g i 6 n g n a y d a d u p c p h d ngCi (chieu ddi m a m tii 1-3 c m ) , s a u d o d u p c t r o n g t r o n g chau vao t h a n g 1 1 / 2 0 0 8 tai n h a luoi c u a Vi^n N g h i d n ciiu Rau q u a . N h i $ t d p n h a ludi d a o d p n g tir 1S^25''C.
2. P h i r o n g p h d p n g h i d n ctiu
T h i n g h i $ m d u o c tiiuc h i d n tir n S m 2008-2010 tien 12 to h o p lai d u p c ki h i e u lan lupt la: A O , AL F O , L O , L F , L O T , L A O T , OTLA, ALA, LAA, O O T v a O T O .
24 NONG NGHllP VA PHAT TRIEN NONG T H 6 N - KY 2 - THANG 4/2012
KHOA HOC CdNG N G H £
Ti^n hanh khu due khi hoa chua no (bao phan chua mo), thuang la truoc khi hoa no tir 3-4 ngdy.
Diing tay tdch nhe dau nu hoa va ngdt cac bao phan (co the diing panh gap bo bao phdn), roi dung bao phan trong cac dia nhua petri, d^ noi kho rao, thodng mat. Khi bao phan bung thi co the su dung hat phdn de tiiu (Van tuyl, 1991).
Doi voi phuong phap thu phan thong thirong:
khi hoa no tii 1-2 ngay (dau nhuy ti^t dich) thi tien hdnh thu phan. DCing biit long (hoac tam hong) chdm vao dia dung hat phan va thu true ti^p l&n ddu nhuy cua cay me. Doi voi phuong phdp tiiu phan cdt voi nhuy: diing dao sdc cdt len phia tr^n hdu nhuy, chi de lai phan voi nhuy co chidu dai 1-2 mm. Sau d6, dung dau nhuy vira cat chdm vdo hdn hpp hat phdn can thu va thu len tren phdn voi nhuy con lai (dinh cua mat cat). Sau khi thu phan xong, tien hanh bao each ly cho hoa bang giay nhom (Van tuyl, 1991).
Doi voi cac to hpp lai xa, tien hanh thu qua 6 cac giai doan 5-7 ngdy, 8-10 ngay, 11-13 ngay sau thu phdn va tien hdnh nuoi cay lat cat bau nhuy tien moi trudng: MS + 90 g/1 sacaroza -i-1 mg/l aNAA Doi voi cdc to hpp lai lai, tien hanh thu qua sau thu phan o 3 giai doan: 3045 ngay, 45-60 ngay vd 60-75 ngdy vd nuoi cay tiii phoi tren moi tnrong Murashige va Skoog (1962) CO bo sung 30 g/1 duong sacaroza va aNAA (0,1-0,3 mg/l), pH=5,8 va de ti-ong didu kien toi b 25''C cho den khi phoi nay mam.
1 . KET qUAVA THAO UJAN
1. Anh huong cua phuong p h ^ thii phdn d^n kha
Thi nghidm su dung phuong phdp thu phan thong thuong vd cdt voi nhuy. Kdt qua o bang 1 cho thay:
Ty 1§ dau qud b cac to hpp lai deu dat khd cao b cd 2 phuong phdp thu phdn thong thuong va cat voi nhuy. Tuy nhidn, co sir khdc bi^t ro rdt vd ty le nay o cdc to hpp lai. Doi vdi cdc to hpp lai xa (tir 1-8), phuang phdp thu phdn cat v6i nhuy dat hi^u qua caa han so voi phuong phdp thu phdn tiiong thuong;
trong khi a cdc to hpp lai lai (tir 9-12) thi phuang phdp thM phdn thong thuong lai dat hidu qua cao hon. Cu thd nhu sau:
O giai doan sau thu phdn 10 ngdy, ddi vdi cdc to hpp lai xa, so qua lai thu dupc khi dp dimg phuang phdp thu phan thong thuong chi dat tir 5-12 qua (tuong ung voi ty le dau qua tir 12,5-30,0%), trong khi dp dung phuang phap thu phan cdt voi nhuy thi ty Id dau qua da tang Idn mot each dang kd (30,065,0%).
Ngupc lai, a cac to hpp lai lai, khi ap dung phuong phdp thu phan thong thuong ty le dau qua lai dat (20,0-87,5%) cao hon so voi khi su dung phuang phap tiiu phdn cdt voi nhuy (10,0-57,5%).
O giai doan sau thu phdn 30 ngay, qua lai ciia cac to hpp lai xa a ca 2 phuang phdp thu phdn ddu khong dupc hinh thdnh (0 qua). O cac to hpp lai lai, doi voi phuong phdp thu phdn cat voi nhuy, ty Id dau qua giam di (chi con 2,5-37,5%), trong khi ap dung phuong phdp thu phdn thong thuong, ty Id dau qua van duy tri nhu giai doan 10 ngay sau thu phdn.
nSng tao qua 6 cdc t6 hpp lai lily
Bang 1. S6 lupng qua lai thu dugc ciia cdc t& hpp lai o phirong phdp thu phdn thdng thudng vd cdt v6i nhuy (sau thu phdn 10 ngdy vd 30 ngdy)
TT
1 2 3 4 5 6 7 8
To hop lai
AO AL FO LO LF LOT LAOT OTLA
So hoa thu phan
40 40 40 40 40 40 40 40
10 ngdy Thu phan thdng thudng So qua lai thu duoc (qua) 5 5 7 6 12
7 0 0
Tyl«
dau qua
(%)
12,5 12,5 17,5 15,0 30,0 17,5 0 0
Thu phan cdt voi nhuy So qua lai thu du(7c (qua) 21 24 26 12 20 24 1 1
Tyle ddu qua
(%)
52,5 60,0 65,0 30,0 50,0 60,0 2,5 2,5
30 ni Thu phan thong thudng So qua lai thu dupc (qua) 0 0 0 0 0 0 0 0
Tyle ddu qua
(%)
0 0 0 0 0 0 0 0
sdy
Thu phan cdt voi nhuy Sd qua lai thu dupc (qua) 0 0 0 0 0 0 0 0
Tyle ddu qua
(%)
0 0 0 0 0 0 0 0
NdNG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 4/2012
9 10 11 12
ALA LAA OCT OTO
40 40 40 40
8 20 25 35
K H O A H p c C 6 N G N C H $
20,0 50,0 62,5 87,5
4 11 18 23
10,0 27,5 45,0 57,5
8 20 25 35
20,0 50,0 62,5 87,5
1 6 10 15
2,5 : 15,0 25,0 37,5 Tidp tuc theo dOi su phdt trien cua qud lai a cdc
to hpp lai lai b giai doan 50 vd 70 ngdy sau thu phdn, nhan thay:
Bang 2. Sd lupng qud lai thu dupc cua cdc t6 hpp lai b phuong phdp thy phdn th6ng thuAng (sau thy phdn
50 vd 70 ngdy)
(7 giai doan 50 ngay sau thu phan, sd lupng qua lai o moi to hpp lai c6 xu hudng giam nh? vd giam m?nh b giai d o ^ 70 ngay sau tiiu phdn. Nguydn nhdn la do ph6i ciia cdc to hpp lai ndy da bi chet 6 giai doan non, hgt bj Idp vd qua bi teo di (bang 2).
2. Anh hu6ng ciia th6i didm ciiu phdi ddn kha nang ndy mdm ciia phdi
Thi nghidm tidn hdnh ciiu phdi cua cdc td hpp lai theo 2 ley thuat: nudi cdy Idt cdt bdu nhuy va nudi cay tlii phoi. Kdt qua o bdng 1 cho thdy ddi voi cdc td hop lai xa, b giai doan sau thu phdn 30 ngay, toan bp sd qud lai da hi khd (phoi bi chdt a giai doan con non).
Do vdy, ddi voi cdc td hpp lai nay da dp dung phirong phdp cihi phoi dua trdn ky thuat nuoi cdy lat cdt bdu nhuy o thoi didm ttr 5-13 ngdy. Ket qua dupc trinh bay 6 bang 3.
Bang 3. Anh hudng ciia cdc giai doan ciiu phdi khdc nhau ddn khd nang phdt sinh hinh thdi cua miu Du6i cdy ciia cdc t6 hpp lai (sau 4 tudn nudi cdy)
To hop lai
ALA LAA OCT OTO
>6 hoa thu phdn
40 40 40 40
50 ngdy So qua Lii tliu dupc
(qua) 6 17 20 31
Tyle dau qua
(%)
15,0 42,5 50,0 77,5
70 ns So qua lai thu duoc (qua)
4 13 12 16
dy Tyle ddu qua
(%)
10,0 32,5 30,0 40,0
Tdhpp lai AO AL FO LO LOT LAOT OTLA
86 bdu nhuy nuoi c^y
6 6 6 6 6 6 6
Tyle miu p.sinh h.thdi («) 5-7nt
41,2 36,7 47,5 38,7 43,3 39,5 42,4
8-lOnt 45,3 40,7 50,6 47,8 47,5 43,3 48,3
ll-13nt 42,4 39,6 48,2 40,8 44,4 42,7 44,1
Tyle mdu tao calus (%) 5-7nt
46,5 42,3 33,4 44,0 34,2 45,9 42,6
8-lOnt 43,7 40,8 32,4 36,1 32,9 43,7 38,6
ll-13nt 46,1 40,8 33,3 41,9 34,0 43,5 41,4
Tyle mau chet
(«)
5-7nt 12,3 21,0 19,1 17,3 22,5 14,6 15,0
8-lOnt 11,0 18,5 17.0 16,1 19,6 13,0 13,1
ll-13nt 11,5 19,6 18,5 17,3 21,6 13,8 14,5 Ghi chii: MTnudi cay latent bin nhuy: MS + 90 g/I sucroza *• 1 nig/Ia NAA
nt ngiy tudi (tinh tir sau thu phan den khi dua vao cuu phdi) Kdt qua 6 bang 3 cho thay tat cd 7 td hpp lai dupc dua vao nudi cdy ddu phat sinh hinh thai theo quy ludt ty le mau phat smh tang ddn va ty Id miu chet gidm dan (theo so ngdy thu mau tir 5 ddn 10 ngay). Tuy nhidn b giai doan tir 10-13 ngdy thi ty 1§
mdu phat sinh hinh thdi giam va ty Id mau chdt tang.
Ty Id phdt sinh hinh thai tiiap nhdt (36,7%) trdn td hpp lai (AL) a thai gian thu mdu 5-7 ngay vd dat ty Id phat sinh hinh thai cao nhat (50,6%) trdn td hpp lai (FO) 6 tiioi didm thu mau 8-10 ngay.
DOI voi cdc td hpp lai lai, tidn hanh thu qua 6 3 giai doan: 3045 ngay tuoi, 45-60 ngay tudi vd 6(^75 ngdy tudi. Kdt qua cho thay sd hii phoi tiung binh/hau nhuy giam dan qua cac giai doan (bang 4).
O td hpp lai ALA, sd tiii phdi/sd bau nhuy nuoi cay (giai doan 3045 ngay tiidi) la 4,7 (trung binh la 7 tui phdi/bdu nhuy), nhung b giai doan 4&-60 ngdy tuoi va 6075 ngay tudi, sd hii phdi tiimg binh/bdu nhijy lan lupt giam xudng con tuong iing Id: 33 tui phdi/bdu nhuy va 2,5 hii phdi/bau nhuy. Td hpp lai OTO cd so tlii phdi/sd bdu nhuy nuoi cay dat cao nhat (115/8), tuong iing vdi 14,4 tiii phdi/bau nhuy.
26 N 6 N G NGHllP VA PHAT TRIEN N 6 N G T H 6 N - KY 2 - THANG 4/2012
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Bang 4. Anh huong ciia th6i didm ctiu phdi ddn sd lupng tiii phdi vd thdi gian ndy mdm ciia phdi a cdc td hpp lai
T d h w lai ALA OOT OTO LAA
Sd tlii phdi/1 bdu nhuy nu6i cdy 3045nt**
4,7 1,2 14.4
3,3
45-60nt 3,3 0.6 13,3
1,9
60-75nt 2,5 0,4 4,2 1,5
Thdi gian ndy mdm ciia ph6i'(ngdy) 30-45nt
37-46 25-38 27-41 30-44
45-60nt 34-42 22-33 22-32 27-35
60-75nt 28-35 21-30 19-28 25-33 Ghi chii: MTnudi cay tui phdi: MS v- 30 g/1 saccaroza + 0,1-0,3 mg/I aNAA
* Thdi gian nay mam ctia phdi: tinh tir khi phdi diu tidn niy mam ddn phdi cudi ciing nay mim kd tu thdi diem bit dau dua vao nudi cay; **nt: ngay tudi (tinh tir sau thu phan den khi dua vao cdu phdi)
T h e o d d i thcri gian n a y m d m c u a p h d i c i i n g c h o thay: k h i t h u q u a 6 giai d o a n s d m h o n tiii t h d i didm bdt d a u nay m d m ciia p h d i m u d n hem vd t h d i g i a n n a y m a m ciia phdi c u n g b i k e o d a i b a n . Tiiy t h u d c t i m g td h p p lai m a thdi g i a n n a y m a m ciia p h d i d a o d d n g tir 1 9 4 6 ngay. O td h p p lai O T O , tiidi didm phdi b a t d a u n a y m a m Id 27 n g a y ( s a u k h i d u a vao nudi c a y q u a 6 3 0 4 5 n g a y tudi) v a k e o d a i d d n ngdy thir 4 1 (trung b i n h la 14 n g a y ) . T r o n g k h i t h u q u a d' giai d o a n 45-60 n g a y tudi, p h o i n a y m a m s o m h o n (ngay tiiii 22 s a u k h i d u a vdo nudi cay) va k e o ddi d e n n g a y t h i i 32 (trung b i n h Id 10 n g a y ) . T h u q u a o giai doan 6 0 7 5 n g a y tudi, p h d i n a y m a m s o m n h a t ( n g a y t h u 19 s a u k h i d u a vdo nudi c a y ) v a p h d i n a y m a m t a p t r u n g h o n ( t r u n g b i n h 8 n g d y ) .
B a n g 5. A n h h u o n g ciia t h d i d i d m o i u p h d i d d n k h d ndng
T d h(7P
lai ALA OOT OTO LAA
n a y m a m c u a p h d T y le p h d i s d n g
(*)
30- 45nt*
63,5 45,2 84,0 55,0
45- 60nt 95,0 65.3 100 75,6
60- 75nt 96,2 70,0 100 77,1
ocac t6h(r plai Ty le phoi nay mdm (%) 30- 45nt
0 4,4 13,0 0
45-
eont
0 8,1 21,3
0 60- 75nt
0 14,3 32,0 0 Ghi chii: MT nudi cay tiii phdi: MS + 30 g/I sacaroza + 0,1-0,3 mg/I aNAA
* nti ngiy tudi (ti'nh tir sau thu phan den khi dua vio cuu phdi)
T y Id p h d i s d n g vd p h d i n a y m d m d- c a c td h o p lai t a n g d a n tir giai d o a n 3 0 4 5 n g a y d d n 6 0 7 5 n g d y tudi do p h d i c a n g d a t d d n d e n d p c h i n s i n h ly (phoi vd ndi n h u c h u y d n tii tiang t h a i l o n g s a n g ti-ang tiiai d o n g d a c ) . T r o n g 4 td h p p lai ti-dn t h i t y Id p h d i s o n g vd p h d i n a y m a m dat c a o n h a t o td h p p lai O T O b giai
d o a n 45-60 ngdy s a u t h u p h d n ( t u o n g u n g Idn lupt Id 100%vd 21.3%) ( B a n g 5 ) .
nr. KET LUAN VAO^ NGHI 1. Kdt l u a n
- D a x a c d m h d u p c p h u a n g p h a p t h u p h a n hidu q u a t r o n g lai g i d n g h o a lily la tiiu p h a n t h e o p h u o n g p h a p t h d n g t h u o n g (ddi vdi c a c td h o p lai lai) v a p h u a n g p h d p t h u p h a n c a t vdi n h u y (ddi voi c a c td h o p lai x a ) .
- T h a i d i e m t h u q u a thi'ch h o p d e d u a vao ciiu p h d i h o a lily ddi vdi td h p p lai x a la 8-10 n g a y sau t h u p h a n v a doi vdi c a c td h o p lai lai la 45-60 n g a y s a u t h u p h a n .
2. D d n g h i
Tidp t u c n g h i e n ciiu x d c d i n h m d i t r u d n g ciiu p h d i thich h p p d e t a n g ty l e p h d i n a y m a m d cdc td h o p lai lily.
TAI UEU H U M KHAU
1. Chi, H . S. (2002). The emciencies of various embryo rescue methods in interspeciSc crosses of hlium. Bot. Bull. Acad. Sin. (2002) 43:139-146.
2. J u o z a s P r o s e v i f i u s , Lidija Stiikulyte (2004).
InterspeciS c hybridization and embryo rescue in breeding of lilies. Acta Universitatis Latviensis.
Biology, 2004. Vol. 676, p p . 2 1 3 - 2 1 7 .
3. K a z u m i K a n o h (1988). Production of inteispecific hybrids between hlium longiflorum x L.
X elegance by ovary sb'ce culture. Japan. J. Breed.
38:278-282.
4. Ki-Byiing Lim (2000). bitiogression breeding through interspeciSc pofyp^oidisation in lily a molecular cytogenetic shidy Phl>fliesis. 27-11-2000,120 pp.
5. Ki-Byung D m & J a a p M . Van T u y l (2006).
hly, Lilium hybrids. C h a p t e r 19, p a g e 517-537. i n :
NONG NGHI|P VA PHAT TRIEN NONG THON - KY 2 - THANG 4/2012 27
KHOA HOC CbNG NGHt
Flower breeding & genetics: Issues, challenges and (1991). Application of in vitro pollination, ovary opportunitiesforthe21stcentury. Springer Verlag. culture, ovule culture and embryo rescue for 6. Van Tuyl J. M., Van Dii M. P., Van Drcij M. overcoming incongruity barriers in interspeciSc G. M., Van Kleinwee T. C. M., Franken J., Bino R. J. hlium crosses. Plant Sci, 74: 115-126.
STUDY ON THE INFLUENCES OF POLUNAllON METHODS AND THE EMBRYOGENESIS TIME ON HYBRIDIZATION FROM DIFFERENT ULY VARIETIES
Summary
Trinh Khac Quang The puipose of this research was to compare the efficiencies of normal and cut-style pollination methods (CSM) for the production of F, and BC; interspecific hybrids of lily. This research included twelve combinations of crosses with six groups (Asiatic-liybrids (A), LA-hybrids (LA). Onental-hybnds (0), OT- hybrids (OT), L fonnolongi (F) and L longWorumThnnh. (L)). Two culture methods-ovary slice culture (OSC) and embryo-sac rescue method (ESR) were used to culture the fertilized ovules or embryo-sac after cut-style and normal pollination on plants respectively. The results have indicated that CSM is superior for the generating F, hybrids. The rate of viable s<tdpods is high (30.0^5.0%). Normal polbnation method is superior for generaUng backcrossing populations with the proportion of viable seedpods peaks at 20.0- 87.5%, For the interspecific crosses, applying OSC method after 8-10 days after pollination brings the highest rate of germinated ovules (40.7-50.696). When using the ESR method, ovanes should be harvested at the best time fi*om 45 to 60 days after pollination (depending on the crossing type and combination), and embryo could be germinated from various genotype combinations (8.1-21.3% success).
Keywords: Normal pollination, cut-style pollination; embryo (sac) culture, ovary slice culture, interspeciSc hybridizaUon; Lilium species.
Ngudi phan bidn: TS. Nguyin Thj Thanh Thdy
NONG NGHIEP VA PHAT TRIEN N 6 N G T H 6 N - KY 2 - THANG 4/2012
