NghUn cdu YDHCT Viit Nam SSSO-2016 DANH GLV DOC TINH CAP VA BAN TRlTOfNG DIEN CUA HATAPHAR
TREN DQNG V4.T THV'C NGHIEM
Study on the acule and sub-chronic toxicity of Hataphar powder u experimental animals
* Ngnyin Thj Tim Thuan ', • • Nguyin Thj Kim Doan' ' Benh vien Yhpcco Iruyin Trung trang ' Benh vien da khoa Chutmg My - Ha Ngi Tomtit
Muc deu: I. Nghien ctiu dgc tinh cdp ctia Hataphar Iren thgc nghiem. 2. Nghien ctiu ddc tinh ban trudng diin da Hataphar trin thuc nghiem. Bdi lugng vd phuang phdp nghien cdu: Nghien cuu dgc tinh cdp Chugt nhdl Irang chting Swiss khoe mgnh, khdng phdn biet gidng. ngng IS -22g.
Phuong phdp nghien ciu: Phuang phdp Lichfield- Wileoxon Nghien ctiu dgc Ilnh bdn tru&ng diin chugt cong (30 can, Chung mslor. Idngtrdng. ngng200±20g Ki, qud nghtln cdu: Bgc tiiih cdp vat Iteu 120 g/kg (liiu gdp tren 16 Idn lieu tdl da dg dinh dimg Iren ngudi). chudt hogt ddng binl,
"••^S sau 72 gld. khdng chit Khdng xdc dinh dugc IDSO.Dfc Ilnh bdn nnrdng diin: vdl liiu IO.S
^S/ngaykao gdp 3 ldn lieu dimg Irin ngudl) gdy dgc tlnh bdn truang dlln Irin chugt d mdc Jj
? « . / / " " ' """•' ""-^ ' '-""".'"" ""•• "^ ' * • • * • ' """""••" **™« Sdy dgc Ilnh cdp. «,- iteu I0.8gkg/ngay (cao gdp 3 ldn liiu dimg grin ngudl) gdy dgc tlnh bdn Irudng diin trin chugt a
Tir Uhoi: Bflt Hataphar. dpc tinh c^p, Mn trirfrng diln Summary
STUDY ON THE ACUTE AND SUB-CHRONIC TOXICITY OF HATAPHAR POWDER IN EXPERIMENTAL ANIMALS.
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^^;!:2^!_^j:^fo^chm^e^nam IradUanal metUdne andpharmacy ( 1D;^T V A N D E
Bgt Hataphar do cdng ty d phfa dugc phtai Ha Tay sta xuit gdm nhtag vj thudc ed tta dung dilu tti hfli chtog da day. Dfa nay ttong nude chua cd mdt nghita cfa tota dita nao vS ChO phim nay. Dl gdp phfa xta dnfli tmh an tota cto cbl phim nay giip cho viec ap dung Iuu flidng si dtog rgng rai ttOn tfij ttutag, chtog tdi tiln htah nghita cfa dl tai: "Dato gia dgc tito cip va bta tmtag difa cia bOt Hataphar ttta dflng vat fliuc
^ p ^ i O m " vdi hai muc tidu:
/• Nghiin cuu dgc Ilnh cap cda Hataphar Irin Ihirc nghiam
2. Nghien ciru dgc tinh bdn trudng diin Iren tho Ihtfc nghifm.
IL CHAT Lifiu, DOI TUgtNG VA PHUONG PHAP NGHIEN CtTU
2.1. Chat lieu nghien cfa: Bgt Hataphar.
- Thato phfa: Cflng thuc cho mflt gdi bgt sta phim la:
6 tjc d t (Os Sepiae): 2,00g.
Triet bli miu (Bulbus fritillariae tfiunbergii): 0,75g,
Ba cim (Herba Hediotidis capitellatae): I,30g,
Cam tfiao (Radix Glycyrrhizae)- 0,75g
MOc huang bic (Radix Saussureae lappae): 0,20g
- Noi sta xuit: Cdng ty cl phfa dugc phim Hi TOy
No 50-2016 2.2. Dli trong vi phuwng phap nghien cfa
2.2.1. Boi lugng nghiin cuu:
Chudt toil ttfag chtog Siviss, ci hai gidng, khoe mato, frgng lugng 18 - 22g/con (100 con) do ViOn VO sito djch te Tmng uong cung cip va chuflt cflng (30 con) chtag Wistar, Iflng ttfag, ntag 200 ± 20g, do Tmng tam cung cip dgng vjt thi nghiem Dan Phugng - Ha lay cung cip.
2.2.2. Phuang phap Uen hanh:
- Xac djto dflc tmh cip: Xac djnh LDjo cia fliulc tfii frfln chuOt toil tttag btag dutag uflng flieo phuang phap Litchfield - Wileoxon [1], [2].
Tmdc khi tiln hito thi nghiem, cho ehuOt tojn ta qua dem. ChuOt nhit ehia 10 Id, mdi Id 10 con, dugc udng mau tfiulc nghien cfa flieo Iilu ttag dfa. Tim lifa cao toit khong gay chet chuflt (0%), lilu flifa toit gay chet chuflt hota tota (100%) va eic liOu ttung gian. Theo doi tito trang Chung cta chuflt va d Iugng chuflt chet d mdi Id trong 72 gid. Tu kit qua fliu dugc xay dung dl thj tayfa tito dl xie djto LDjo cua tfiudc thu. Sau dd, tiOp tiic tfieo doi tito ttang chung cia chugt den hit ngay fliu 7 sau khi udng tfiuflc flii Ifa diu.
- Bflc tt'to bta ttutag difa: chudt cdng chtog Wistar (30 con), dugc chia ngta nhita thato 3 Id, mli Id 10 con, moi con todt rieng mdt chudng:
NghUn cdu YDHCT Vie, Nam Lol (Chtag sito hgc) (n-lO):
Uflng nudc cat Iml/IOOg/ngay Lfl tri I (n=IO): Uing Hataphar lieu 3,6 g/kg/ngay (lieu cd tdc dung luang durmg tren ngtral, tinh theo hf sd 6).
Lfl tti 2 (n=10): Udng Hattiphar lifa 10,8 g/kg/ngay (gfa 3 ifa Id tti 1)
Chugt dugc udng mrac hojc thudc thi frong 4 tata IiOn, mfli ngay mflt Ita vao buOi stag.
2.2.3. Hoa chat, dung cy dung trong ngbien cthi:
Dung djch xet nghidm man ABX Minidil LMG cua htag ABX Diagnostics, djto Iugng ttta may Vet abc™ Animal Blood Counter vi XC - 55 chemistty analyzer (Tmng Quic), cta phta tich cia Nhat.
2.2.4. Chi lieu theo tldi:
Tai cic thdi dilm tmdc lie uing thuoc, sau 2 tufa vi sau 4 tafa uing fliuflc, chuflt cong chtag Wistar dugc kiem tt-a cac thdng sl:
- Tito tt-jng chung, khdi Iugng co flil.
- Bato gia chic ntag tao mau:
flidng qua sl Iugng hdng ciu, sd Iugng bgch ciu, cdng fliuc bgch ciu va sd Iu- tag tieu ciu.
- Dato gia chic ntag gan, tfita, vi tfll
2.3. Dja diem vi thai glan nghita cfa
- Bja diim nghiin ciru: Bd mdn Dugc Iy Tmtag Dji hgc Y Ha NOi
- Thdi gian nghiin citu: t i flitag 3/2015 din flitag 5/2015.
2.4. Phiromg phap xir ly so lifa:
theo thujt tota thflng kO y sito hgc IH. KET QUA
3.1. Dge tinh cap cfa Hataphar Chuflt toit ttfag dugc uflng thule flii Hataphar ttr lilu fliip toit dfa lilu cao toat. Lfl chugt da uflng din lieu 120 g/kg tfll ttong chuflt taong duong vdi hiu gip ttdn 16 ifa Iilu tii da dg djto dtog tten ngudi toung khdng co dOc tito cap (tito hfl sd ngogi suy tten chugt toit la 12). Chua xac djto dugc LD50 fren chuOt toit frfag cia fliulc thi Hataphar fren dutag uflng [3].
3.2. Danh gii dgc tinh ban trufag dien:
3.2.1. Bdnh gid Irin tlnh trgng chung:
Trong tfioi gifa flii nghigm, chupt d 16 chfag sito hgc hogt dpng blnh fliutag, fa uing tflt, toato togn, long mugt, mit stag, phta khd. ChuOt d 2 Id uong Hataphar an uong kem, vjn dOng chjm. Sau 4 tufa nghien cfa co 3 chuflt d Ifl uong Hataphar Iilu 10,8 g/kg/ngiy bj tiOu chiy va ehlt. Cac chuflt chit da dugc ml va kilm tta dji thi cta CO quan diu khflng quan sit J thiy cd thay dli bOto Iy nao vl mjt Si dai the cia cac co quan tim, phli, gan, ^ lach, tuy, thta vi hg thflng tieu hoi cia ^
chuOt. ^ 3.2.2. Bdnh gid trin sif thay doi
thi trgng chugl:
^^a^lflff^f^^^^j^J^ traditional medidne ami phamuuy Na SO - 2016 g j l ^ g j j - ^ liirfag eua H a t a p h a r d i n t h i ttgng chuot
Thdi gian
Tmdc udng thudc
Lo c h m g (c) (n=10) Trgng
Iuyng (g) J i S D
Sau 2 tafa udng thule
> ttudc - sau [Sau 4 tafa J u i n g fliudc l p ttudc - sau
170,50 ± 39,19 187,50 ±
39,10
<0,05
% thay doi trgng lirgng
194,50 ± 35,63
Lo tri 1 ( n=10) Trgng iugng (g) 3r±SD
r 10,63
T 15,35
<0,05
176,00 ± 29,89
% t a n g trgng lirong
Lo tri 2 (n=10) '?' T r g n g
Iirgmg (g) S"±SD
170,00 ± 35,36
>0,05 180,63 ±
37,46
>0,05 i3,87
i0,13
171,70±
20,33 138,89 ± 26,67**
<0,05 1 5 9 3 ±
29,78*
>0,05 i 18,55
14,84
tang so vol ttuoe khi nghien cfa, cd i nghia flidng ke tn < 0 05^ T.„ i . -
^ c a c Id uing Hataphar c f l x u b u t a g ^ g i a r s o v d i t L ^ k l ^ S ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ fr 2 uong Hataphar lita cao cd mfa gitai cd y nghla flilnVke s ^ ; ! tt^fl k i nghien cuu va so vdi Id chfag sito hge (p < 0,05).
3.2.3. Bdnh gta chuc nang tgo mdu:
Thdi gian
S l Inyng h i n g c i n ( T/l ) L6trj2 LS chfag(c) Lo fri 1
(n=10) I (n=10) P(c-l) p(e-2)
NghUn cdu YDHCT Viil Nam SSSO-2016 Nhta xet: Sau 2 tafa uflng
Hataphar, sfl lugng hflng cau d ca id tri 1 (uflng Hataphar lieu 3,6 g/kg/ngay) va Ifl tti 2 (uflng Hattiphar lilu 10,8 g/kg/ngay) ttag so vdi tmdc khi uing thuflc va ttag so vdi id chtag sito hgc cd su khic biet vdi p < 0,05. Tai thdi
diem sau 4 tafa udng thuflc, sfl Iugng hflng ciu d 2 Id tri cd xu hutag giam so vai flidi dilm sau hai tafa uing tfiuflc, so vdi thdi dilm ehua uing thuflc va so vdi Ifl chfag sito hgc tfii su khac biOt khflng cd y nghTa Uilng ke (p > 0,05).
Btag 3.3: Ato hufag eua Hataphar den so lugng bach cau trong man chugt
Thdi gian
Tmdc uing fliulc Sau 2 tata udng thude
p (tmdc-sau) Sau 4 tafa udng thudc
p (tmdc-sau)
s l lirgng bach can (G/l) Lo chfag
(n=10) X i S D 6,41 ± 1,24 7,44 ± 1,44
>0,05 7,46 ± 1,32
>0,05
Lo tri 1 (n=lb) JT ± S D 7,29 ±1,71 8,37 ±2,16
>0,05 8,23 ±1,45
>0,05
Lfl trj 2 (n=10) X ± S D 7,44 ± 1 , 1 0 11,47 ± 2 , 9 7
<0,05 7,90 ±3,21
> 0 , 0 5
P (c-1)
>0,05
>0,05
>0,05 p(c-2)
>0,05
<0,05
>0,05 1
o -j^.™, j« luuiig upi-11 ^au u lo Ul ^ laiig CO y ngnia so vdi thdi dilm ttudc khi uflng thudc va so vdi Ifl chfag sito hgc (p < 0,05).
Sau 4 tuta uflng Hataphar, sl Iugng bach ciu d 16 ttj 2 (uflng Hataphar Iilu 10,8 g*g/ngay) giam di so vdi thdi dilm 2 tafa, khflng co su khac bigt cd y nghTa tiling ke so vdi Id chfag sito hgc va so vdi thdi dilm tmdc uong thuoc (p > 0,05).
Bang 3.4: Anh hufag cua Hataphar din sl lugng tieu can trong miu chugt
Thdi glan
Tmdc udng fliudc Sau 2 tata uflng
thudc p (ttirdc - sau) Sau 4 tufa udng
thuoc p (ttudc - sau)
s l lugng tilu can IG/lt Loch fag
(n=10) A - i S D 517,80±157,84 587,50±107,57
>0,05 538,60±70,17
>0,05
L o t r i l (n=10) X i S D 620,00± 124,46 638,78±132,79
>0,05 725,75±I3I,25
>0,05
Lo tri 2 (n=10) X ± S D 535,20±154,04 599,00±I29,25
>0,05 768,43± 187,91
<0,05
p(e-l)
>0,05
>0,05
<0,05 p(c-2)
>0,05
>0,05
<0,01
jf^^-frr^archlnvletjmn^gaiiSomdmedidneamlphamu.cy Na 50 - 2016 Nhta xft: Id frj I sau 4 tafa udng Hataphar, d lugng tifa cfa d ed xu h u t a u
Itag tomig 31, khta biet khflng cd i nghla tfitag kO vdi p>0,05 ^ Lo frj 2 sfl Iugng tita cfa ttag ro so vdi Id chfag sito hoc va so vdi tfidi dilm
ttuoe uong tfiudc ( p < 0,05). vm moi aiem 3.2.4. Bdnh gld mure do huy hogi tibdo gan vd chuc ndng gan:
! f ; i j f i i ; ^ j ; ; [ g ; g c u a Hataphar dfa hoat do AST trong m i u ehudt
ThMgian
Hoat do AST (UM)
Tnrac u6ng thudc Smi 2 tuin u6ng thuoc
P (tnroc - sau) Sau 4 t u ^ u6ng thu6c
Lfi chirng
£n=10) Jr±SD 95,10±12,81 102,4a±22,44
>0,05 106,80±11,17
>0,05
Ld tri 1
£n=I0) X ± S D 107,20±15,11 113,44±16,99
>0,05 118,64±19,16
>0,05
L 6 f r i 2 Oi=10) X ± S D
p(c-l) p(e-2)
106,90±16,13 130,33±44,76
>0,05 122,86± 12,23
>0,05
>0,05
>0,05
>0,05
>0,05
<0,05
<0,05 p (audc - sau)
chupt^khong CO sg khta bigt so vdi chfag sito hpc va so vdi tmdc udng . h u l c v «
ho. ! ; ° , ! ^ - ^ r " ^ ' " ^ " " ^ " ^ ' ^ " ° ' ' " " " ••' -^^T ' ^ « •=" y "ghIa so vdi Ifl chfag sito hpc va thm diem ttudc uflng fliuta vdi p<0,05, tomig sau 4 tafa uflng fliuficto a d l A ^ T t t a g ^ k h o n g c o . n g h r a so vdi I d c b t a g sito hpc v i flidi dilm fr^dcuflngthui:
Btag 3.6: Anh hirfag eiia Hataphar dlnhoatdfl Al T , „ „ g niiu chugt
NghUn edu YDHCT Vie, Nam SSSO-2016 Nhta xet; Ld tri I sau 2 tufa va 4 tufa udng Hataphar, hogt do ALT trong mau
chuflt khdng cd su khic bigt so vdi Id chfag smh hgc vi so vdi tnrdc uing thuoc vdi p>0,05.
Ld tti 2 sau 4 tafa udng thudc, boat dfl ALT frong mau chupt eo xu hutag ttag so voi lfl chfag smh hgc va so vdi ttudc khi uflng fliudc flii, sg khae biet cd y nghla fliing ke vdi p<0,05.
3.2.5. Anh hudng cda Hataphar din nong dg creatinin Irong mdu chupt Btag 3.7. Anh hirfag eia Hataphar den nong dO creatmm trong man chugt
Thdi gian
Trudc udng thudc Sau 2 tuta udng thule
p (trudc - sau) Sau 4 tuta udng thudc
p itntcK - sau)
Creatinin (mg/dl) Lochfag
(n=10) X i S D 1,06 ±0,05 1,04 i 0,05
>0,05 1,07 ±0,05
>0,05
L o t r i l (n=10) X i S D 1,05 ± 0,05 1,07 ±0,05
>0,05 1,04 ±0,05
>0,05
L o t r ! 2 (n=I0) X i S D 1,06 ± 0 , 0 4 1,04 ±0,05
> 0 , 0 5 1,07 ± 0 , 0 5
> 0 , 0 5 p(c-l)
>0,05
>0,05
>0,05 p(c-2)
>0,05
>0,05
>0,05
uflng Hataphar, d ci Id tri 1 (Uing Hataphar lifa 3,6 g/kg/ngay) va 16 ttj 2 (Uing Hataphar lilu 10,8 g/kg/ngay), n6ng dfl creatinin ttong mau chuflt khflng cd sg khac biet ed y nghla thflng ke so vdi Id chfag va so sato giSa hai thdi dilm ttudc va sau khi udng tiiudc thi vdi p>0,05.
3.2,6. Thay dii vi mo bfnh hgc sau 4 luan uing thuoc:
* Thay dii vi dgi thi:
TrOn tit ca cac chugt fliuc nghiem (ea Ifl^ chfag va 2 Id frj), khdng quan sat fliiy cd fliay dfli beto Iy nao vl mat dai flie eto cac co quan tim, phli, gan,
lach, tuy, thta va hO tiling tiOu hoa cua chuOt.
* Thay dii vi vi Ihi:
+ Hito thai vi thi gan:
Lfl chfag: Hinh tah tl bao gan bito thutag va Uioii hfla mic dfl toe.
Lfl tti 1 (uing Hataphar 3,6g/kg/ngay): Hito ito tl bio gan bito thutag va thoai hda mic dg tog.
Lfl ttj 2 (uing Hataphar 10,8 g/kg/ngay): Hmh ato tl bta gan thoai hda mfa dg tog vi via
+ Hito fliii vi flil flita:
Ld chfag: Hito tah thjn binh thutag.
103
Jaumal of research In vie, nam Iradidanal medicme andpharmacy Ld tti 1 (udng Hataphar 3,6
g*g/ngay): Hmh a t o flita bmh tfiutag.
Ld tti 2 (udng Hataphar 10 =
Na SO-2016
g/kg/ngay): H t o ato flita bmh tfiutag I V . B A N L U ^ :
4.1. Ve dgc tinh c i p
Sau khi uing tfiudc fliu Hataphar cho fliiy: vdi Iilu g i p ttta 16 ifa Ufa tfli da du djto dtog fren ngudi toung khdng ed dgc tito cip (tito he s l ngoai suy fren chuflt toit la 12). Chua _ ! ^ d j t o dugc LDsn fr6n chuOt toit B ^ H c u a fliudc flii Hataphar frOn
dutag uong theo phuang phap Litchfield - Wileoxon, Kit qua niy chtog td tfiulc bdt HATAPHAR khdng ed dgc tmh [3], [4], [5], [6).
4.2. DOc tinh bin trufag diln cua hOt Hataphar
4.2.1, Anh hudng cua bgl Hataphar din Ilnh trgng chung cia chupt
Theo h u t a g dfa cia To c h i c y t l flie gidi, tito ttgng chung, ttgng lugng CO the v i cac chi sfl huylt hgc la tofag xet nghiOm bit buOc khi dato gia dgc tmh cto fliuflc tfii. Trong flidi gian tfli nghigm, chuflt d 10 chfag smh hgc boat dflng bito thutag, fa uing tot, toato togn, Iflng mugt, mit stag, phta khd Chupt d 2 Id uing Hataphar fa uing kem, v t a dflng chjm. Sau 4 tafa nghien cfa cd 3 chuflt d Id udng Hataphar lilu 10,8 g/kg/ngjy bj tieu
chay va chit. Cac chudt ehlt da dugc md va kilm tt-a dai flil cac co quan diu khdng quan sat tiiiy cd fliay doi bOto Iy nao v l mat dai tfil e i a cac co quan tim, phdi, gan, lach, tuy, flita va he thdng tieu h o i c i a chuflt.
* Boi vdi thi Irgng cda chugl:
Sau 2 tafa v i 4 tafa uing fliuflc tfiu, frgng lugng chuflt d Ifl chfag sito hge ttag so vdi hudc khi nghiOn cfa, cd y nghTa tfiong ke (p < 0,05). Trgng lugng chuflt d cac Ifl uflng Hataphar cd xu h u t a g giim so vdi ttudc khi nghiOn ciu, dac biet d Id udng Hataphar lilu cao cd mfa giim cd y nghla tfidng ke so vdi ttudc khi nghien cfa va so vdi Id chfag smh hgc (p < 0,05), la do chuflt ta it nOn frgng lugng c i a chuflt giimdi.
4.2.2. Bdnh gia kit qud dtfa via chic nang tgo mau:
Ket qua d btag 3.2 cho thiy: Sau 2 tata uflng Hattiphar, s l lugng hflng eau d ca Id tii 1 va Ifl fri 2 dfa ttag cd su khac biet so vdi Id chfag sito hgc (p<0,05) la do biOn taong cfl djc mau, cd mflt sfl chuOt bj mit nudc do ia chay. Nhung sau 4 tafa uing thuic, sfl Iugng hong ciu d 2 Id hi cd xu hutag giam so ydi tfidi dilm sau hai ttifa uong tiiulc, so vdi thdi dilm chua uflng tfiulc v i so vdi Ifl chfag sito hoc tiu su khac biet khflng cd y nghTa fliflng ke (p > 0,05) la do chugt da tfioat khdi tito frang ia chay mit nudc.
NghUn cdu YDHCT Viit Nam
sd lugng hdng can cd xu hutag frd ve bito thutag.
Kit qua d btag 3.3 cho tiiiy: Sau 2 ttifa udng Hattiphar, sl lugng bgch can d lfl tri 2 ttag cd y nghTa so vdi thdi dilm tmdc khi uflng tfiuOc vi so vdi 10 chtag sito bgc vdi p < 0,05 Ii do biOn taong cd dac mau, cd mgt sl chuflt bi mit nudc do ia ehay. Sau 4 tata udng Hataphar, sl lugng bach ciu d Ifl trj 2 giam di so vdi tiioi dilm 2 tata, khflng cd su khic bigt co y nghTa tiling ke so vdi Id chfag smh hgc va so vdi flidi dilm ttudc uing fliulc vdi p>0,05. Nhu vjy tfiudc bflt HATAPHAR vdi lilu 3,6g/kg/ngay vi lieu 10,8g/kg/ngay khdng ato hutag den sd lugng bach ciu ttong mau chuflt.
4,2.3, Bdnh gid kit qud dira vao muc dghuy hogi ti bag gan:
AST va ALT la hai enzyme trong huyet fliato cd ngudn goc gan dugc dtag dl dato gia mic dfl tin fliuong te bao gan. Khi cd tin thuong hiy hoai tl bao gan, kl ca khi cd fliay dli tito tfiam cia mtag tl bao gan flii hogt dfl ALT da cd thi tfag ien. Khi tin Among tl bao gan d mfa do dudi tl bao AST mdi dugc giai phdng va ttag lta. Vi vay ttong tfa tfiuong gan ho,t dg ALT fliutag ttag cao hon AST [7], [8]. Trong nghien cfa nay ctog cd biOu hign tou vjy.
So SO-2016
T i tofag phta tt'ch kit qua d hai btag 3.5 va 3.6 cd flil dato gia chung la fliuflc bOt HATAPHAR vdi Ufa 3,6g/kg/ngay khdng gjy ito hutog den te bao gfa chupt.
4.2.4, Bdnh gid kil qud difa vao chii-c nang thgn:
DO dato gia ato hutag cua fliuoc dOn chic ntag tfita cin phai dito Iugng ndng dfl creatinin huylt thato [7], [8], Creatinm huylt thato khong bj phg tiiuOc vao chi dp. ta va totog tfiay dli smh Iy, no chi phu tfiuflc vao khi ntag dao fliai cua flita. Nlu cau tfifa bj tfln tiiucmg creatinin huylt tiiato tfag tmdc khi ure ttag. Do dd creatinin huylt tfiato la chi sd lin cjy vi qufa ttpng hon chi sl ure khi dato gia chuc ntag thta.
Kit qua d btag 3.7 cho thiy: sau 2 tata va 4 tafa uflng Hataphar, d ca lfl tri I vi Id tri 2 ndng dp creatoin ttong huylt fliato chuflt thay dli I^ac biet khflng cd y nghTa flifag k6 1 so vdi 16 chfag va so sato giua hai .•<;
flidi dilm ttudc va sau khi uflng •«
fliulc thi (p > 0,05). Nhu vay thuoc j bpt HATAPHAR vdi Iilu -, 3,6g/kg/ngay va 10,8g/kg/ngay -«
Miflng ato hutag din ohic ntag j
thta cia chupt. j 4.2.5. Binh gld kil qud dfra vdo •'%
stf Ihay dot vi mo bfnh hgc:
Sau 4 tuta uflng fliuflc ve dai flil tten ,.
tat ci cta chugt fligc nghiOm (ca Id
Jaunm^research in vie, nam Chung vi 2 Id tti), khflng quan sit fliiy cd fliay ddi bOtoly nao vl mat dai All eia cac CO quan tim, phdi, gan, lach, tay, flian va hfl fliflng tfeu hoa cua chuflt
Sau 4 hifa uflng tfiuflc cd bilu hita khta nhau vl su tiiay ddi hito Mi yi flie gan: (3 Ld chfag vad Id tti 1 (udng Hataphar 3,6 g*g/ngay) fliiy hmh ato to bio gan bito fliutag va flioai hda mue dp toe. C) Id tti 2 (uing Hataphar lilu 10,8 g*g/ngiy) tfiiy hito ato tl bao gan thoii hda mic dfl tog va via.
• f K E T QUAN
5.1. Dgc tinh eip: Bflt Hataphar HiOng gay dflc tito cip tren chuflt toit
traditional medldrK andpharmacy Na SO-2016 ttfag khi cho thu vdi lifa 120 g/kg (liOu gfa tten 16 ifa Ufa tfli da dg djnh dtog ttdn ngudi) chua xie dito dugc LDji, fren chuflt toit frfag cia tfiuflc tfli Hataphar fren dutag ufing flieo phuang phap Litchfield - Wileoxon.
5.2. Dgc tinh bta trufag dien:
Mau fliuflc fliu Hataphar dang fliuflc bgt lilu 10,8 gAg/ngay (cao gip 3 ifa Iilu dtog fren nguoi) giy dflc tinh bta ttutag difa tten chufit d muc dO toe khi cho chufit ufing ttong 4 tafa lien tuc bifa hiOn chuflt bj tieu chay, ttog boat dfl ALT va tfag tin fliuong gan ttOn vi till, 30% chuOt chit.
TAI LIEU THAM KHAO 1- Bg Y tl (2005), Chf thdo hutmg
ddn thtr dgc tinh cua thudc Cdc phuang phdp thir dpc tinh cdp 0£CA Phy Igc 2,
2- Gerhard Vogel H. (2002), Drug discovery and evaluation Pharmacological assays. Springer.
3. Gerhard Vogel H. (2008), Drug discovery and evaluation Pharmacological assays, Sprmger
4. BO Y Tl (2010), Dupc diin Vift Nam IV, Nha xuit bta Y Hpc Ha Nfli, fr,739-922
5. WHO (1993), ••IVorking groupon the safety and efficacy of
herbal medicine". Report of regional office for tiie western pacific of flie WHO, March, pp. 33-51
«• Vt'HO (m3),'']Vorking groupon the safety and efficacy of herbal medicine". Report of regional office for flie western pacific of flie World Health organization.
7. Nguyin Thi Khanh, Phgm Tir Duong (2001), Xet nghifm sir dgng trong Idm sang, Nha xuit bta Y hpc.
8. Vii Dinh Vinh (2001), Huang ddn sti dvng cac xet nghigm sinh had, Nha xuat bta Y hgc, fr 115-287.
