Tap chi Khoa lioc 2012.23b 115-124 Tnrdng Dgi boc Cdn Tha
KHAO SAT HIEU QUA HA D t 6 N G HUYET
\ A CHONG OXY H 6 A CIIA CAO CHIET CAY NHAU {MORINDA CITRIFOLIA L.) 6 CHUOT
BENH T l£ U DL'^OfNG
Gdi Thi Xudn Trang', Xguyin Thi Mai Phuang', Vo Thi .\goc Diim' va Quach Tii Hue' A B S T R A C T
Hyperglycemia increase in oxidative stress results in diabetic complication. This study evaluated the anti-diabetie potential ofethanolic exiracis from root, leaf, green and ripe fruits of Morinda eitrifolia by determining their antioxidant and antihyperglycemic activities in vitro and in vho. The antioxidant activity was determined tlirough the measurement of DPPH radical scavenging actiYity in vitro. Mice were induced diabetes by using alloxan monoliydrate. The extracts of Morinda eitrifolia could suppress hyperglycemia in diabetic mice by the oral intake. Finally, the antioxidant effect of these plant extracts in the kidneys of experimental diabetic mice was obsen-ed through the total antioxidant status ITAS) in kidney of mice. The results showed that oral administration of ethanolic extracts (200 mg/kg x 2 times/ day) could make lower the glucose le^-el of blood as well as the commercial antidiabetic drug fglucofast). and reduced oxidative stress- mediated damage in diabetic kidneys. The results also proved that Morinda eitrifolia ethanolic extracts improved ihe DPPH radical scavenging activity in vitro.
K^'words: Diabetic, DPPH, TAS, antihyperglycemic, Morinda eitrifolia
Title: Studies on hypoglycemic and antioxidant activities of ethnolic extracts from Morinda eitrifolia L. in diabetic mice
TO-M T.AT
Tdng duang huy-et lam gia long sir tgo thdnh stress oxy hda la nguyen nhdn cua nhiing bien chimg phiic tgp a benh lieu duang iBTDi. Xghien ciru nay khdo sal hieu qud dieu tri BTD cua cao ethanol re. Id. trdi xanh vd trdi chin cay Shau ihong qua kha nang chong oxy hoa vd hg dudng huyet in vivo va vi vitro. Hogt dong chong o.xy hoa duac .xdc dinh tiidng qua klid ndng ldm sgch gdc tudo DPPH in vitro. Chuot duac gd}' benh lieu dudng bdng dung dich allo.xan monoliydrale (.AM). Sau do. chuot BTD duoc dieu tri b0nh rai cao ethanol cdc bd phdn cdy .\hdu. Than cua nhom chuot dieu tri BTD duoc khdo sdt hieu qud chdng oxy hoa thong qua khd ndng chdng oxy hoa tdng sd (TAS). Ket qua cho thdy. sau khi chuot BTD udng cao chiei cdy Xhdu vdi lieu luang 200 mg/kg '<2 ldn/ ngd}- cd dudng huvet tuang dutmg nhdm chuot BTD dirac dieu tri vdi thudc tri tieu duang glueofast vd gidm thieu stress oxy hoa trong than. Ket qud cOng chung minh rang dich chiet ethanol cd\- .Xhdu cdi thien khd nang ldm sgch gdc tudo DPPH in vitro
Tir khda: B?nh tiiu dir&ng, DPPH, TAS, su tang dudng huyit, cay Nhau
1 GlOfI T H I E U
H i e n n a \ B T D d a n g p h i t t r i e n r i t n h a n h v a I i n g u y e n n l i a n c u a n h i e u b i e n c h i i n g phijrc t a p ( B h a t et a7.. 2 0 0 8 ) . S u p h i t t r i e n ciia B T D d o n h i l u n g u y e n n h i n t r o n g d d
nguyen nhan chinh la stress ox\ hda. Stress ox)' hda din den su tao thinh cac gdc
' Khoa Khoa hoc Tv nhien. Tnrong E)ai hpc Can Tho
Tap chi Kboa hgc 2012:23b 115-124 Trudng Dgi hgc Cdn Tba
tu do la \k\x td chinh cua su phat triln benh va nhimg bien chung phuc tap cua BTD (Tri'pathi and Chandra. 2009). NgSn chan sir tiln trien ciia BTD bang each bd sung cac chat chdng oxy hda tu nhien hien dien trong thuc vat do cac chit chdng ox-\ hda nay cd khi ning iam sach cac gdc tu do cd hai cho c a t h i tir su stress o.xy hda (Pale? a/.. 2011).
Ciy Nhiu {Morinda eitrifolia L.) va cac bd phan ciia ci\- dugc cic nha khoa hgc dac biet quan tim trong nhiJng nam gin diy. Cay Nhau chda nhieu hgp chit chdng oxy hda nhu beta- carotene, acid ascorbic, terpenoid alkaloids, beta sitosterol, carotene, polyphenol nhu flavonoid, flavone glycosides, rutin... (Ramamoorthy and Bono. 2007). Ci> Nhiu dugc bilt cd hieu qui. gia thanh thip Iai it tic dung phu nen se la mdt trong nhthig loai thudc thuc vat tri BTD trien vgng (Ramamoorthy and Bono, 2007).
2 PHU'CfNG TIEN VA PHU'ONG PHAP NGHIEN ClTU 2.1 Phiroiig t i | n
Thilt bi dugc su dung trong nghien cuu gdm may cd quay chin khdng Heidolph (Dire), may do dudng huylt ACCU - CHECK^ Active, may Iy tam lanh Mikro 220R (Dire), miy do pH Metier Toledo, may lie 8 vi tri Daiki SIOOl (Han Qudc).
may do quang phd. may khuay tir. may vortex.
Hda chit sii dung trong thi nghiem gdm ethanol (China). DPPH (2.2-T3iphenyI- l-picr>ihydrazyl) (Wako. Japan), hydrogen peroxide (HiOi) (Merck). EDTA (Merck), Fe(NH4)2(804)2 (Merck), acid acetic, alloxan monohydrate (AM) (Sigma), Trolox (Denmark), nudc mudi sinh ly. sodium benzoate, TBA (thiobarbituric acid) (Merck), thudc dilu tri benh tiiu dudng glueofast. diethyl ether va mgt sd hda chat khac.
Vat lieu thi nghiem la chudt nhat trang do vien Pasteur tiianh phd Hd Chi Minh cung cip. R I , la, trai xanh va trai chin ciy Nhau dugc thu hai d tinh Kien Giang.
2.2 Phirong p h i p
2.2.1 Trich eao edy Nhau bang dung moi ethanol
Nguyen lieu dugc su dung bao gdm li, r l . trai xanh va trii chin cua cay Nhau dugc thu hai d tinh Kien Giang. Cac mau dugc rua sach bang nudc sau do dugc cit nhd.
de rieng. phoi khd cho den khi trgng lugng khdng ddi.
250 g mdi loai li, r l . trai xanh \ a chin cua ciy Nhau khd dugc ngam trong ethanol 70% d nhiet do phdng. Sau thdi gian 7 ngay, phan nudc dich ngam m i u dugc Igc va cd dudi dung mdi dudi i p suit kem. Cao ethanol tir cay Nhiu sau khi loai bd dung mdi dugc trft d 4"C de sir dung cho thi nghiem sau.
2.2.2 Khdo sdt hogt dong lam sgch goe tir do DPPH in vitro
Hoat ddng lam sach gdc tu do ciia cac cao ethanol tir cac bg phan ciia ciy Nhau dugc thuc hien theo quy trinh ciia Shirwaikar et al. (2006) cd dieu chinh nhu sau:
Cao ethanol cic bd phin cua cay Nhau dugc pha thanh cac ndng do la 0,25: 0.5:
0.75: 1; 1.5; 2 va 3 mg/ml trong ethanol. 200 pi cao chiit d mdi ndng do khio sat dugc them vao 100 ^il DPPH 6.10"^M. Hon hgp phan img sau khi li 60 phut d nhiet dd phdng v i de trong tdi dugc do do h i p thu quang phd d budc sdng 517 nm. Hoat 116
Ti^ chi Khoa hgc 2012 23b 115-124 Tnrdng Dgi hgc Cdn Tha
ddng Iam sach gdc tu do DPPH dugc tinh toan tucmg duang fiM Trolox (Koracevic et al. 2000).
2.2.3 Khdo sdt khd ndng hg dirang huyet cua eao ethanol ede bo phgn cdy Nhau Chudt 9 tuin tudi khde manh dugc tiem dung dich alloxan monohydrate (AM) d ndng do 130 mg/kg trgng Iugng chudt de giy BTD. Sau khi chudt BTD dn dinh 7 ngay, kha n i n g ha dudng huyet cua cao ethanol la, re. trai xanh va trii chin ciy N h i u dugc xac dinh bang each cho chudt BTD udng thudc dieu tri B'TO glueofast (150 mg/kg trgng Iugng chudt) hoac cao ethanol tir cac bd phan cua ciy Nhau (200 mg/kg trgng Iugng chugt). Chudt BTD udng cao ethanol la, rl, trai xanh va trii chin 0,1 mlx2 lin/ngay trong 21 ngay dieu tri. Dudng huyet dugc xac dinh vao liic 8 — 9 gid sang trudc khi chudt dugc cho i n . Sau khi do dudng huylt 60 phiit, chudt dugc cho an va udng nudc binh thudng. Sau 21 ngay dugc dieu t n benh vdi cac cao chiet, chugt dugc cho nhin ddJ qua dem. thin dugc Iiy ra v i xu Iy cho cic thi nghiem tilp theo.
2.2.-f Khdo sdt su chong oxy hoa long so (Total Antioxidant Status assay (TAS)) in vivo
K h i nang chdng oxy hda ciia cao chiet ciy Nhau dugc thyc hien dya tren ngu\en t i c sau: Dung dich sodium benzoate ket hgp vdi Fe(NH4)2(804)2 tao ra cac O2"
(superoxide). Neu trong thin cd hien dien cac chat chdng oxy hda thi se ket hgp vdi cac superoxide. Lugng superoxide cdn Iai se ket hgp vdi TBA de thanh lap TBA — RS (thiobarbituric acid reactive substances) dugc do d budc sdng 532 nm.
Quy trinh phan ung dugc thyc hien theo cac budc sau; 100 mg than chudt dugc tich ra nghien min vdi 200 ^l dung dich dem phosphate lanh ndng do 100 mM.
pH = 7,4. Sau khi Iy t i m trong 10 phut d 10.000 vdng/phiit, phin dich ndi d tren duoc sir dung cho viec khao sat hoat tinh chdng oxy hda cua cao chiet (Bhat et al.
2008).
TAS dugc x i c dinh theo phuong phap cua Koracevic et al. (2000) cd hieu chinh nhu sau: 10 ^L dich thin sau khi dong nhit (kidne\ homogenate) dugc pha loing vdi 490 |J.L dung dich dem sodium phosphate ndng do 100 mM. pH ^ 7,4 dugc cho vao hdn hgp gdm 0.5 mL dung dich sodium benzoate 10 mM vdi 0.2 mL Fe - EDTA (2 mL Fe - EDTA dugc pha tii 2 mM dung dich EDTA vdi 2 mM dung dich Fe(NH4)2(S04)2). Sau dd 0,2 mL H2O2 10 mM dugc cho vao hdn hgp phan ung. lie d i u va u d ST^C trong 60 phiit. Sau khi u, 1 mL acid acetic 2 0 % v i TBA (thiobarbituric acid) 0,8% trong NaOH dugc them vao dng nghiem. Hon hgp phan ung sau khi ii d 100°C trong 30 phiit dugc de ngudi d nhiet do phdng. Do h i p thu quang phd cua phin img dugc do d budc sdng 532 nm. TAS trong than dugc tinh toan tuang duang ndng do mM Trolox (Erejuwa et al, 2011).
2.2 5 Thdng ke phdn tieh so lieu
Sd lieu dugc trinh bay bing MEAN ± SEM. Ket qua dugc xit Iy thdng ke theo phuang p h i p ANOVA bing phin m i m minitab 16.0.
Tap chi Khoa hgc 2012-23b 115-124 Tnrdng Dgi bgc Cdn Tba
3 KET QUA VA THAO LUAN
3.1 Sff an toan cua cao ethanol cac bo phan cay Nhau tren chu6t binh thiromg Dl dinh gii su an toan (khdng giy ddc tinh cip) ciia cao ethanol cac bd phan cay Nhau chudt nhit tring binh thudng dugc cho udng cao ethanol a nong dg 200 mg/kg trgng lugng. Kit qua vl sy thay ddi dudng huyet cua chugt dugc trinh bay trong hinh 1.
Theo kit qua hinh 1 cho thiy, dudng huylt cua chudt udng cic cao chiet va nhdm chudt ddi chimg thay ddi khdng khac biet cd y nghia thdng k& gifta cac nhom vi cac ngay udng cao chiit trong sudt qua trinh thir nghiem 21 ngiy. Nhu vay cao ethanol cac bd phin cay Nhiu khdng anh hudng din dudng huyet chudt binh thudng. Mat khac. trgng Iugng chugt sau 21 ngay udng cao ethanol cac bd phin ciy Nhau cung khdng khic biet cd y nghia thdng ke so vdi nhdm ddi chirng (ket qui khdng trinh bay).
Hinh 1: Nong d§ duong huyet cua chu^t khi u6ng cac cao chiit d nong do 200 mg/kg tr^ng lu-ong
Tir tat ca kit qui trinh bay tren cho thiy cao ethanol cac bd phin cay Nhau khdng gay ddc tren chudt binh thudng d ndng do 200 mg/kg frgng lugng frong 21 ngay.
3.2 Kha nSag h? dirong huyet cila cac cao chiet tir cic b6 phan cay Nhau tren chuot b€nh tieu dvong
Ndng dp dudng huyet ciia chudt sau 7 ngay dugc tiem dung dich AM vao khoang bung dugc trinh bay frong bang 1. Kit qua cho thiy ndng do dudng huylt cd sy khac biet cd y nghia thdng ke (P < 0,05) khi tiem AM ndng do 130 mg/kg trgng Iugng so vdi chugt d nhdm ddi chting khdng tiem AM.
Bang I: Nong d$ duvng huyet ciia chugt truoc va sau khi tiSm alloxan monohydrate Nghiim thi^c Trirfrc khi tiem AM Sau khi tiem AM Doi chiing
Tiem AM
124,33 ±4,04 133,44± 13,95
121,33''±1.15 421,94" ±65,90 Chl chil cac chit cdi iheo sau irong ciing m il Ididc nhau se khdc biel coy nghia thdngke am
Qua 21 nga\ dieu fri, ket qua cho thiy d nhdm chudt binh thudng udng nudc cat dudng hu\ et gin nhu dn dinh frong sudt qui trinh thi nghiem (khdng cd khac biet
Tgp chi Khoa boc 2012:23b 115-124 Trudng Dgi bgc Cdn Tho
cd j nghTa thdng k^ P > 0,05). Ddi vdi cac nhdm chudt BTD khdng dugc dieu tri xuat hien chudt chet rii rac sau 2 ngay va t i t c i chudt chet sau 9 ngay thi nghiem.
Nhdm dieu fri bang tfaudc glueofast cd ndng do dudng huyet giam cao nhit la 67,65%, Trong cac nhdm chudt dieu tri bing cao chiet cay Nhau thi nhdm dieu tri bang cao r l cd hieu qua cao nhit (giim 58,01%), sau do la nhdm dilu tn vdi cao frai chin (giam 51,62%), cao trai xanh (giam 41.43%) va cudi ciing Ii cao Ii (giim 34,21%).
B a n g 2: BtroTig huyet cua cac n h o m nghidm tbirc triFVC va sau khi dieu t n benh tiiu d u o n g Nghiem thire
Binh thuang KJiong tri benh Glueofast Re La Trai xanh Trai chin
Dircrng huyet (mg/dl) Triro'c dilu tri Sau diiu tri 121,33"* 1,15 1 3 2 , 6 r ± 11.85 464,33" ±76,81 Chet 477,00" ±28,58 154.33'±4.04 443,00"±53,81 186,00"* 16,52 3 81,00" ±61,61 250,67" ±22.03 385,33" ±90,05 225.67"''± 45.24 381,00'±21,28 184,33" ±57,66
Ty le tang (+), siam (-) (%) + 9,35 - 67.65 -58.01 -34.21 -41.43 -51.62 Chl chu- cdc chQ cdi iheo sau irong ciing mgl cgl khdc nhau si khdc biet cd v nghTa ihdng ki a muc 5%.
Theo nghidn cuu cua Pham Thi Lan Anh (2011) fren chudt BTD dugc giy benh bang AM va dieu fri vdi cao chiet r l Nhiu vdi lieu Iugng 400 mg/kg trgng Iugng.
su dung 0,1 ml/2 lin/ ngay trong 20 ngay dieu tri dudng huyet gjim 71,62%. Nhu vay hieu q u i ha dudng huyet ciia cao ethanol r l Nhau dugc su dung frong nghien ciiru nay thip han (58,01%) do lieu Iugng cao chiit dugc sir dung thip han (200 mg/kg frgng Iugng chugt).
Nhieu cao chiet thuc vit ciing dugc chiing minh cd khi ning ha dudng huyet fren chuOt b^nh tieu dudng. Trong dd kha nang ha dudng huyet ciia mudp ding {Momordica charantia) (giam 51,2%). cd ca ri {Trigonellafoenum graecum) (giam 55,2%) (Tripathi and Chandra, 2009) tucmg img vdi cao r l Nhiu (giam 58,01%) va cao trai chin (giam 51.2%) frong nghien cuu nay. Tuy nhien kha nang ha dudng huyet cua cao chiet tir cic bd phan cay Nhau thap hem so vdi nho do {Vitis vinifera variet>' Burgund mare) (giam 65,33%) (Chis et al, 2009) v i ciy la dira (Pandanus amaryllifolius R.) (giam 73,2% khi sir dung ndng do 400 mg/kg frgng Iugng chudt) (Pham Thi Lan Anh, 2011).
3.3 K h a o sat ho^t tinh ch^ng oxy hoa ciia cao chiet cay Nhau
3.3.1 Khdo sdt khd ndng ldm sgch gdc tir do DPPH (2,2~Diphenyl~l-picrylhydrazyl) cua eao chiet tir ede bo phdn cdy Xhdu in vitro
Xac dinh kha ning chdng oxy hda bing phuang phap DPPH la phuang p h i p dan gian, nhanh chdng va it tdn kem. Trong nhimg nam gan day phuang phap niy Cling dugc sir dung d l djnh Iugng chat chdng oxy hda frong cic he thdng sinh hgc phuc tap (Prakash et al, 2000). DPPH la hgp chat cd miu tim dugc phat hien d budc sdng 517 nm. Khi cac dien di le cua cic gdc ty do DPPH kit hgp vdi hydro tir chat chdng oxy hda thi se hinh thanh nen DPPH-H liic nay m i u sic chuyin tir mau tim sang mau vang. Sy bien ddi mau nay tuong ung vdi Iugng electron ket hgp vdi DPPH (Prakash et al, 2000). Do dd, khi ning lam sach gdc ty do ciia mdt
Tap chi Khoa hoc 2012:23b 115-124 Trirdng Ogi hoc Can Thr,
chSt cang cao thi sir hjp thu quang ph6 duoc do a 517 nm cua phan ung DPPH co gia tri cang giam va ngugc lai.
Trolox (Vitamin E) la chat chdng oxy hoa chuin thuang dugc sir dung de so sinh kha nang lam sach g6c tu do ciia cac chSt cSn dugc khao sat. Duong chuan DPPH dugc tinh theo ^ M Trolox theo phucmg Uinh duong chuan la y = -0,0096x + 1,6226 (R^ = 0,9918).
Kha nang lam sach gdc tu do o cac ndng d6 cCia cao ethanol cac bg phan cay Nhau tinh tuong duong theo n-M Trolox dua vao phucmg trinh duong chuin, kjt qua dugc trinh bay trong bang 3.
Bang 3: N^ng dp cua cao chiet rl, la, trai xanh va trai chin cSy Nhiu tinh tircmg dinmg Trolox
Nong do cao chiit (mg/ml) 0,25 0,5 0,75 1.0 1,5 2,0 3,0 ECso (mg/ml)
Gi^ tri nong dd i C a o r l 21,38±4,25 34,14 ±3,71 50,19 ±3,67 74.31 ±2,61 93,59 ± 2,93 105,05 ±1,36 133,64 ±1,56 1J531 ±0.003
cao theo chuin Trolox (|iM) CaoU 36,77 ± 6,38 61,01 ±6,54 81,67 ±8,28 100,5 ±7,21 130.44 ±4,87 145.66 ±5.62 153.96 ±4,98 0,9172 ±0.007
Cao trdi xanb 8,68 ± 1.99 28,22 ± 0.93 65.49 ± 0,55 70,41 ±1,97 100,66 ±0.95 137.73 ±0,94 157,55 ±0,6 1,0252 ±0.001
Cao tr^i chin 59.5 ± 4,38 122,39 ±2,8 150,8 ±0,43 155,11 ±0,6 155,67 ±0,38 160,35 ±0,49 162,78 ±0,5 0,2369 ±0.002 Kit qua cho thiy kha nSng lim sach gdc ty do ty Ie thuan vdi ndng do ciia cao ehilt. ndng do ciia cao chiet cang cao thi k h i nang Iim sach gdc t y do cing ldn va ngugc Iai. Kha nang Iam sach 50% cac gdc ty do EC50 (Effective concentration of 50%) dugc tinh toan dya vao dd thi va kit qua dugc trinh biy frong Bing 2. Trong do cao chiit frai chin cd kha nang lim sach gdc ty do cao nhat (ECso= 0,2369 ± 0.002) sau dd l i cao la (EC5o= 0.9172 ± 0,007), cao trai xanh (EC5o= 1,0252 t 0.001) va cudi cimg la cao rl (EC5(,= 1,253 ± 0,003).
Phin tram do hip thu DPPH ciia cao ethanol cac bd phin ciy Nhau dugc thi hien d hinh 2 cho thiy khi ndng do cao chiet ting thi do hap thu DPPH ciing ting.
Trong dd, phin fram do h i p thu DPPH cua cao frai chin l i Idn nhit d ndng do 0,25 mg/ml (36,57%). thap nhit la cao frai xanh (7.14%). 0 ndng do 3 rag/ml cao ehilt, do hip thy DPPH cua cao frii chin l i 96,39%, thip nhit la cao re 79.51 %.
Tap chi Khoa Ape 2012 23b 115-124 Truang Dot hoc Cdn Tha
025 0 5 0.75 1 1.5 2 3 N6oE dg cao cbiet (Big/ml)
Hinh 2: Kha nang lam sach goc to-do ciia cao ethanol cac bp phSn cay Nhan (%) EC50 ciia cao l i frong phan iing DPPH theo nghien ciiu cua Thani et al (2010) (0,31 mg/ml) thip hon so vdi cao la sii dung trong thi nghiem nay (0,91716 mg/ml) vi cd the cao la dugc su dyng trong thi nghiem cua Thani el al (2010) la cao ethanol 95%. Dich chiit ciia la Nhau thu dugc tir nhung k-y thuit tich chiet khic nhau ciing dugc sii dung de khio sat kha nang Iim sach gdc ty do DPPH theo nghien cuu cua Pak-Dek et al. (2011) ciing phii hgp vdi gia tri EC50 cua cao Ii frong nghien cuu nay.
3.3.2 Khdo sdt khd nang chong oxy hoa long sd (Total Antioxidant Status (TAS) assay) in vivo
Than la mdt trong nhung co quan ciia c a the bi i n h hudng bdi sfress ox\ hda din den nhimg bien chiing phiic tap, nen sy loai bd cac gdc tu do cd hai bdi nhirng chit cd kha ning chdng oxy hda ty nhien nham ngan chin nhirng bien chiing phuc tap ciia BTD cung la mdt trong nhiing myc tieu dugc lya chgn de hudng tdi kiem soat BTD hieu qua (Pah et al, 2011). Kha nang chdng oxy hda ciia cao chiet ciy Nhiu dugc khao sat d than ciia chudt BTD theo phucmg p h i p TAS. Ket qua ve k h i ning chdng oxy hda ciia cao ethanol tir cic bg phan cay Nhau dugc tinh tuong duong theo mM Trolox dya \ ao dudng chuan va phuang trinh dudng chuin y ^ -0.3923x + 1.1822 (R^ = 0.9902). kit qua dugc trinh biy frong bang 4.
Kit qua d bang 4 cho thiy nhdm chudt BTD khdng dugc dieu tri cd kha nSng chdng ox> hda thap nhit (0.5240 ± 0.0123 mM Trolox) cd khac biet cd y nghia thdng ke so vdi cic nghidm thiic cdn Iai (P < 0.05). Kha nang chdng oxy hda dugc cii thien d cic nhdm dugc dieu tri benh vdi glueofast va cac cao chiei. Trong dd nhdm chudt dilu tri bang glueofast cd k h i ning chdng ox\ hda tuong duang vdi nhdm chugt binh thudng udng nudc c i t (khdng cd khic bi^t cd y nghia thdng ke P > 0,05). Kha nang chdng oxy hda ciia nhdm chudt BTD dieu fri vdi cao r l va cao frii chin tuong duong nhau. nhdm chudt BTD dilu tri vdi cao Ii tuang duang \ d i nhdm dilu tri vdi cao trai xanh. Tu\ nhien. cac nhdm dieu tri vdi cao ethanol tir cac bd phin ca\ Nhau deu cd khac biet cd y nghia thdng ke so vdi nhdm chudt binh thudng udng nudc cat (P < 0.05).
Tap chi Khoa fipc 2012:23b 115-12-i Tnrimg Dgi hoc Cdn Tha
Bang 4: Hieu qua chdng oxy hoa cua cao chiet tir cac hQ p h a n c than chuot bfnh tieu dutrng
NghiSm thirc Hieu qua chong oxy hoa dir^c tinh theo mM Trolox
iy Nhau dir^c khao sat it Hieu qua chong oxy boa
diroc tinh theo ty l | % Binh thuong
Benh Idiong ui Glueofast RS La Trai xanh Trai chin
1.1218"± 0.0215 0,5240''±0.0123 1,0963" ±0.0015 1,0536''±0,0051 1,0120'±0,0256 1,0251 "±0.0259 1,0522''±0,0497
8833 40.48 86.29 82,88 79,55 80.59 82,76
Ghi chu cdc cha cdi iheo sau trong ciing mgt cgl khdc nhau se khdc biel co y nghta ihdng ki d muc 5K.
Lugng gdc tu do da ket hgp vdi chat chdng oxy hda cd frong thin ciia chudt tuong img d cac nghiem thiic dugc the hien frong bang 4 va hinh 3. Ket qua cho thiy phan tram Iugng gdc tu do kit hgp vdi chit chdng oxy hda cao nhit d nhdm chugt binh thudng udng nudc cit (88.33 %) v i thip nhit d rihdm chudt BTD khdng dugc dilu tri (40.48 % ) . Ddi vdi cac nhdm chudt BTD dugc dilu fri vdi glueofast (86,29%) va giam dan d cac nhdm chudt BTD dieu tri vdi cao chiit ciy Nhau lin lugt la re (82,88%). trai chin (82,76%) frai xanh (80,59%) v i la (79.55%).
1 1
i i i I
• J J i 1 1 •
Bmh KhoDg in Glueofast Re La Tiai xanh Trai chin thuong tienh
N^iem thi>c
ITinh 3: Ph3n tram lirong g6c t u d o ket hop v ^ chat chong oxy hoa trong than Theo nghien cuu cua Erejuwa er al. (2011) chupt BTD dugc diSu trj voi thu6c diSu trj BTB nhu metformin hoac glibenclamide kdt hgp voi mat ong cai thien dugc tinh Uang sUess ox> hoa 6 than hieu qua hem so vdi chi su dung thudc diSu tri metformin hoac glibebclamide.
Tir ket qua nghien cuu dat dugc cho thSy cao chiet tir cac bg phan ciia cay Nhau co hieu qua on djnh duong huySt a chugt BTD cung nhu loai bo duoc cac tac nhan gay ra stress oxy hoa o than ciia chupt BTD. Didu nay cho thSy hieu qua ciia viec sii dung cay Nhau nhu lieu phap ho trp trong di6u tri BTD. Day chinh la thong tin khoa hgc huu ich cho cac benh nhan BTD lua chon cay Nhau ii ho tro diSu tri bJnh. Nghien cilu nay cung gop phSn b6 sung co so khoa hoc cho v hoc c6 truyen nhung du lieu hen quan den viec img dung cay Nhau trong dieu tri benh.
T^ chi Khoa hoc 2012 23b 115-124 Tnidng Dai hoc Cdn Tho
4 K E T LL AN VA K I E N N G H I 4.1 K i t luan
- Cao ethanol cua cay Nhau cd tic dung ha dudng hu> It fren chudt BTD sau 21 ngay dilu tri theo thir hr r l (58.01%), frai chin (51.62%). trii xanh (41.43% va l i (34,21%).
- Hieu qua loai bd gdc ty do ciia cao c h i ^ Nhiu theo thii ty trai chin (EC50 ^ 0Ji37 ± 0,002), la (EC50 ^ 0.917 ± 0.006). frai xanh (EC50 = 1-025 ± 0.001) v i re(EC5o= 1.531 ±0.003).
— Phin n^m lugng gdc t y do tao ra d than eua chudt benh tieu dudng ket hgp vdi cac chit chdng ox>' hda trong cao chiet ca\ NTiiu theo thii ty lin lugt trii chin (82.76%). r l (82.8'8%) frai .xanh (80.59) \'a la (79.55%). Hi^u qua loai bd gdc ty do ciia cic cao chiit Nhau urong duang vdi glueofast (86.29%) va gin bing vdi chudt khde manh (88.33" o).
4.2 Kien nghi
Khao sit cic c a che ha dudng huyet ciia cao chiet ciy Nliiu fren chudt benh tieu dudng.
— Khao sat c a che chdng ox\ hda cua cao chiet cay Nhau d cac c a quan khac nhau ciia chudt benh tieu dudng.
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