Nguyin Hai Linh vd Dtg Tgp chi KHOA HQC & CONG NGHE 164(04). 195-199
C A O C A Y S i r O N G S A O (Mesona chinensis B e n t h . ) C O T A C D ^ J N G H O T R g f D I E U T R I B E O P H I T R E N C H U Q T N H A T T R A N G
NguySn Hai Linh', Ma Di^u Quynh', Ma Thj Thu L^', Biii Thi Thu Thuy', Vu Thj Minh H&ng', Nguyen Thi Hong Hanh^*
' Trudng Viing cao Viet Bac, Thai Nguyen. ^ Trudng Dgi hpc Supham Ha Noi, Hd Ndi TOM T A T
Suong sao (Mesona chinensis Benth.) la mgt loai cSy trong pho bien d trung du va mien mii phia Bac nude ta, cay co nhieu tac dung dugc ly quan trgng. Nghien cuu dugc thuc hien nham tach chilt va dinh gia tac dung cua cao cay Suong sao trong vi?c ho trg dieu trj beo phi trSn d^ng v|t thyc nghi?m, Cao cay Suong sao dugc dinh lugng Polyphenol, Flavonoid tong so va thu nghi?m tr6n 96 chugt nhat trang beo phi (chung Swiss) voi lieu uong tang dan tir 100 - 500 mg/kg the trgng trong 6 tuin. Ket qua dinh lugng cho thay ham lugng Polyphenol va Flavonoid trong cao cay Suong sao lin lugt la 375 mg/g cao va 265,6 mg/g cao. Sau 6 tuan, chupt duoc ulng cao djch chi6t Suotig sao CO su giam khoi lugng ca the dang ke, Trong do, lieu uong 400 mg/kg the trgng co mdc giam khoi lugng cao nhit, giam so vdi thdi diem trudc dieu tri la 9,04 g hay giam 13,4% (P<0,05).
Nhu vgy, cao cay Suong sao c6 tie dyng h i trg dieu tri beo phi'tren chugt nhat trang.
Tir Ichoa: Mesona chinensisBenth.. beo phi, chupt nhdt trdng. Polyphenol, Flavonoid.
DAT VAN D E
Beo phi dang trd t h i n h t h i c h thdc y te dang bao dgng khong chi d c i c n u d e phat t n e n m i con d cac n u d e dang phat tnen bdi ti le ngudi mac beo phi dang gia tang mdt each nhanh chong, gay ra nhirng i n h h u d n g khdng nho tdi sde khoe, tam ly, hieu s u i t lao dgng. Beo phi con la nguyen nhan true tiep dan den c i c benh man tinh n h u cao huyet ap, tinh mach, viem xuong k h d p , . . . d i e biet la roi loan lipid mau va d i i thao d u d n g [9].
Polyphenol l i mgt hgp chat chdng oxy hda t u nhien dugc tim thay trong mgt sd l o i i thuc vat. Trong mot sd t h i p ky gan d i y , da cd nhieu nghien c u u danh g i i t i e dung chong oxy hoa va u c c h e a-glucosidase cua Polyphenol t u thuc vat [7]. Trong s6 cac loai dugc t h i o thien nhien, S u a n g s i o (Mesona chinensis Benth.) la mot logi cay cd nhieu t i e dung d u g c ly quan trpng. M o t sd nghien cdu d i chi ra r i n g cay S u o n g sao co tac dyng quan trgng trong viec dieu tri beo phi n h d hgn chi cam giac them an v i h i p thu chat dinh dudng [4]. S u o n g sao cd c h u a 17 loai axit amin (trong dd,c<^ 7 axit amin thiet y l u ) , carbohydrate, c h i t beo, c h i t xo. Polyphenol
va Flavonoid [8], [11]. O n u d e ta, Suong sao d u g c trdng pho bien d vung Trung du va Mien nui phia Bac ciing n h u mgt sd tinh mien N a m . T d mdt loai cay hoang dgi, n g i y nay S u a n g sao d u g c trong d n h i l u dia p h u a n g n h u n g quy md con nho. S u a n g s i o d u g c sd dyng trong s i n xuat thach den - mgt logi do udng thanh nhiet. Tuy nhien, cho den nay, tgi Viet Nam, chua co nghien c u u ve t i c dyng d i l u t n beo phi cua cay Suong s i o . Do dd, nghien cdu nay d u a c tien h i n h nham muc tieu x i c dinh ham lugng Polyphenol v i Flavonoid tong so trong cao Suong s i o td dd d i n h gia tac dung cua cao cay S u a n g s i o trong viec h6 tro dieu tri benh beo phi tfen-chuOt n h i t tring.
V A T LIEU, PHU'ONG PHAP NGHIEN C U U V a t lieu nghien c u u
- M i u thuc vit: Cay S u a n g s i o (Mesona chinensis Benth.) dugc thu hai vao t h i n g 6 nam 2016 tai huyen Djnh Hoa, V o Nhai cda tinh Thai Nguyen. Sau khi thu hai, cay S a a n g sao dugc say kho, bao quan d nai khd rao, nhiet do 2 5 - 3 5 ^ .
- M i u ddng vat: 208 chupt n h i t t r i n g (Mus musculus), chung Swiss 6 t u i n tu6i (20 - 22 g), do Vien Ve sinh dich t l T W cung c i p . ' Tel 0902.801.1S3
Nguyen Hai Linh vd Dtg
Tgp chi KHOA HQC & C 6 N G N G H $164(04): 195-199 Phuong phap nghien ciru
Phuffng phdp nuoi chuot: Chugt dugc nudi trong Idng d nhiet do 25 ± 2''C, do im 55 ± 10%, chieu sing 12 gid/ngay. Thdc in cho chugt dugc cung cap bdi Vien ve sinh dich te Trung uong vdi thanh phan cic chat dinh dudng theo tieu chuan cua AIN76 [5] gdm:
Cacbohydrate 260 kcal/100 g, protein 85 keal/100 g, lipid 45 keal/100 g thuc in.
Phuffng phdp tgo cao cay Suang sao: Thin va la cay Sucmg saa sau khi thu hai dugc rda sgch va siy khd den khdi lugng khdng doi d 60°C. Miu khd dugc nghien thinh bgt min.
25 g bOt khd dugc trdn deu va tich chiet bang 200 ml dung dich ethanol (80%) d 60°C trong 16 gid. Sau do, dich chiet dugc lgc trong dieu kien hut chan khdng vao binh thdy tinh. Tien hanh co dgc dich chiet thu dugc eao cay Suang sao.
Phuang phdp dinh luang Polyphenol vd Flavonoid: Him lugng Polyphenol t6ng sd dugc xic dinh theo phuang phip Folin- Cioaltea [12]. Ham lugng Flavonoid tdng so dugc xac dinh theo phuang phap cda Cui. va es(2010)[6].
Phuffng phdp thu dgc tinh cdp LDsa: Dinh gia tinh an toan ciia cao cay Suang saa bang phugng phap thu ddc cip tinh LDso cda Litchfield (2000) [10].
Phuffng phdp gay chuot beo phi thuc nghifm: Quy trinh gay chugt beo phi thuc nghigm dugc thuc hign tren 32 chugt nhit tring 6 tuan tudi chia lam 2 Id thi nghiem (16 chugt/ld): Id (1) - binh thudng: An khiu phan in co sd do Vien Vg sinh Djch te Trung uong cung cip vi 16 (2) - beo phi:
An khau phan an chda 40% lipid (an khau phin co sd, 100 g md Ign, 20 g cholesterol tinh khiet trpn vdi cam nong). Khau phan thuc an duge xiy dung dua theo Bang thanh phin dinh dudng Viet Nam,
Phuang phdp xdc dinh khoi lugng chupt:
Sd dyng can dien tijr dl cin chugt. Khdi lugng chugt d cic Id thi nghiem dugc theo doi trong 6 tuin,
Phuongphdp thd- nghiem tdc dung hd trffdieu tri beo phi cua cao cay Suong sao: Thi nghiem thuc hien trSn 96 con chudt da beo phi (khli lugng 66,9 - 68,0 g). Chugt dugc chia thanh 6 16 thi nghiem (16 chupt/ld), trong do:
- Lo 1 - Id ddi chung (DC): Dugc udng nude cit lOml/ngay
- Ld 2 - Id dilu tri 1 (DT 1): Udng cao djch chiet Suang sao 100 mg/kg can nang/ngay - Ld 3 -16 dieu tri 2 (DT 2): U6ng cao dich chifit Suang sao 200 mg/kg can nang/ngay - Ld 4 - Id dieu tri 3 (DT 3): Udng cao dich chiet Suang sao 300 mg/kg can nang/ngay - Lo 5 - 16 dieu tri 4 (DT 4): Uong cao dich chiet Suong sio 400 mg/kg cin nang/ngay - Ld 6 - Id dieu tri 4 (DT 5): Udng cao dich chiet Suang sao 500 mg/kg can nang/ngiy Hang ngiy, chugt dugc udng cao dich chiet vio bu6i sing trudc khi an khau phan co sd 30phut, Cic 16 chupt dugc theo doi khli lugng trong 6 tuin dieu tri.
Xii' ly sl liSu: Cic s6 heu dugc bilu thi dudi dgng: Gia tri trung binh ± dg Igch chuan ( X ± SD), dugc xd ly th6ng ke tren phan mem MS. Excel vdi muc y nghia P < 0,05,
KET QUA VA T H A O LUANKet qua djnh luong Polyphenol vi
Flavonoid tong s6 trong cao cay suffng sao
Tu 25 mg mau Suang sag kho chung t6i da
thu dugc 0,64 g cao cay suang sao, cd mau
nau dim, khdng mdi. Khi thuc hien quy trinh
tag cao ciy suang sio, do methanol co tinh
dgc hai, vi vay nhdm nghien ciiu sd dyng
dung mdi etanol thay thi va vin tgo dugc cao
ciy Suang sao dim bio chit lugng. Ham
lugng Polyphenol va Flavonoid trong cao cSy
Strong sao dugc thi hien d Bang 1.
Nguyin Hai Linh vd Dtg Tap chi KHOA HQC & CONG NGHE 164(04): 195-199
Mau bgt cay Sirinig sao kho
Bang L Hdm luang Polyphenol vd Flavonoid trong cao cdy Sucmg sao Tong cao
Sirtfng sao thu duyc (g)
H i m luyng Polyphenol tone so fg)
Tfl?
Polyphenol tong so (%)
Ham lugng Flavonoid
(g)
T i l f Flavonoid
(%)
Ti l§ Polyphenol tdng sd trong eao cay Suang sao chiem khoang 37,5%. Kit qui nay chenh lech vdi ket qua dinh lugng Polyphenol trong nghiin cuu trudc diy [4] li 15%. Su chenh lech cd thi giii thich do miu ciy Suang sao dugc trdng va thu hai d nhdng vung dia ly khac nhau din din su sai khic vl kit qua.
Chdng t6i tilp hic so sinh kit qua trin vditi II Polyphenol trong cac loai la da dugc nghien cuu nhu la 6i (23,4%) [I], li sen (20%) [II]
da dugc nghien cuu, Nhu vay, him lugng Polyphenol trong cao ciy Suang sio thu hai tai Thai Nguyen tuong ddi cao. Do dd, diy li mgt ket qua tuang ddi kha quan dl chung tdi tiep tyc nghien ciiu de tai.
Trong thanh phin cic hgp chit Polyphenol, Flavonoid la mgt nhdm chit da dugc nghien cdu cd vai quan trgng trong viec bao ve co thi, chdng cic gdc oxy hda, ha lipid miu [3].
Vi viy, chdng toi tiep tuc tiln hanh dinh lugng Flavonoid trong cao cay Suang sao vi thu dugc ham lugng Flavonoid chilm 26,56%
cao cay Sucmg sao.
Ket qua danh gia sy an toan cua cao cay Strong sao
Sau khi thd nghiem tren 80 con chu^t ulng cao Suang sao tu 100 mg/kg din 8000 mg/kg khoi lugng ca the, sau 72 gid theo doi bieu hien trang thai sue khde cda chupt, chupt khong CO bieu hien suy ylu hoac chit, cac
26,56 hoat dpng sinh ly cda chugt diln ra binh thudng gidng nhu nhdm chudt khdng udng cao suang sio. Chung tdi khdng tim thiy lilu dpc cap tinh LD50 Dieu dd chdng td cao ciy Suong sao khdng giy ddc cho chupt thuc nghiem bing dudng udng.
Ket qua xay dung md hinh chu$t beo phi
Sau 6 tuin xay dung mo hinh chudt beo phi thuc nghigm, chung tdi nhan thiy. Id chugt thuc nghigm co khiu phin an giau lipid (40%
lipid) tang khdi lugng Idn ban rat nhilu so vdi 16 chupt ddi chung an khiu phin ca sd (12% lipid) va su sai khic nay la cd y nghia thing ke (P < 0,05). Sau 6 tuin nudi, chudt ddi chiing tang 17,18g (ung vdi ting 85,09%) so vdi khdi lugng ban diu), trong khi chugt dugc nuoi beo phi tang 46,97 g (dng vdi tang 230,25% so vdi khdi lugng ban diu). CJ tuin thii 6, khdi lugng trung binh cda 16 thyc nghiem beo phi da len den 67,37 g, trong khi dd, d 16 doi chihig khdi lugng trung binh chi li 37,37 g. Kit qua dugc thi hign d Bang 2.
Nhu vay, vdi cdng thdi gian nudi (sau 6 tuan nuoi), chupt an thdc an cd ham lugng lipid cao da tang khoi lugng hon chupt an khau phin ca sd li 30 g iing vdi 80,28% hay gip 1,8 lin [2], Cd thi kit luan chdng t6i da thanh cong trong viec tao m6 hinh chuot beo phi thyc nghiem.
Bang 2. Khdi lugng trung binh cda chupt Id binh thuang vd Id bio phi tnr&c vd sau 6 tudn n Lo chu^t
(n = I6)
TutoO(g)
Tuan 6 (g)Kh6i lucmg ting sau 6 tuan (g) Ti 1? IdiSi luc^ng tang sau 6 tuSn {%)
Lo binli thir^ng ( X ±SD) 20,i9±0.29 37,37±I,79 tl7,18*
tS5,09*
Lo beo plii ( X ±SD) 20,40±0,67 67,37±2,23 T46,97«
1230,25*
(Chti thich: J: giam. T: lang: •p<0,05:"p> 0.05)
Nguyen Hai Linh va Dig Tsp clli KHOA HOC & CONG NGHE 164(04): 195-199 Bang 3. Khoi lufmg trung binh cita cdc 16 chugt 16 thi nghiem Iruac va sau diiu tri L5 chu^t
( n - 1 6 ) Truac dieu trj (g) Sau dieu tri (g) Miic tang, giam (%)
L 6 D C (X ±SD) 67,44±2,15
76,70±1,52
tl3,73«
L 6 B T 1 ( ^ ±SD) 67,63±1,86 66,65±1,78 i l , 4 5 "
LflDT2 67,75±1,54 65.66±1,23 i3,08««
L a D T 3 f-^ ±SDt 67,37±2,19 62,37±3,71 17,42 •
L 6 D T 4 fX±SD) 67,46±2,24 58,42±1,91 413,4*
L 6 D T 5 (X iSDI
66,9±1,88 58,67±1,S5 J12,3 * (Chii thich: J;gidm, T: tdng; *P< 0.05; ** P> 0.05;
K e t q u a d a n h gia t a c d g n g hh t r g dieu trj b^o p h i c u a c a o cay S u o n g sao Sau 6 t u i n u6ng cao dich c h i l t S u a n g sao d cac lilu l i n lugt la 100, 200, 300, 400, 500 mg/kg can nang, s u thay ddi khdi lugng c o the chugt d u g e the hien d B i n g 3.
K i t qua d Bang 3 cho thay, khi khdng d u g c dieu tri b i n g cao S u a n g sao, kh6i lugng c a t h i cua Id chudt D C tang 13,73% so vdi khoi lugng ban dau trong khi dd, c i c Id chugt d u g c dieu tri bang cao cay S u a n g sao cd su g i i m khdi lugng. Chugt dugc u6ng cao S u a n g sao lieu 100 va 200 mg/kg can nang cd khoi lugng ca the giam lan lugt l i 1,45% va 3,08%
so vdi trudc dieu tri, tuy nhien su khac biet la kh6ng CO y' nghia thong ke. Lo DT 3 (udng cao Suong sao lieu 300 mg/kg can nang) g i i m khdi lugng 7,42% (P< 0,05). Ld DT4 va DT5 cd m d c giam kh6i lugng c a the cao va g i n t u a n g d u a n g nhau, lan lugt la 13,4% (P<
0,05) va 12,3% (P< 0,05) so vdi khdi lugng ban d i u . K i t q u i n i y cho t h i y cao cay S u a n g s i o CO t i c dyng trong viec hd trg dieu tri beo phi t r i n chu^t nhat trang.
N h u vay, d lieu dieu trj 400 mg/kg can nang, khdi lugng chupt giam mgnh n h i t trong c i c 16 dieu tri, m d c dp giam cao hem 16 DT3 l i 5,98%, DT5 la 1,1%. N h u vay, l i l u d i l u tri 400 mg/kg can nang la lieu tdi uu d6i vdi viec d i l u trj beo phi.
K E T LUAN
Nghien ciiu da tach chiet d u g c cao cay S u a n g sao b i n g etanol 8 0 % . T d 25 g m i u S u a n g sao khd da tao d u g c 0,64 g cao cay suong s i o , tir do x i c djnh d u g c ham lugng Polyphenol trong cao cay S u a n g sao la 3 7 , 5 % , ham l u g n g Flavonoid l i 26,56%. K i t 198
DC: Ddi chung; DT: Diiu tri)
q u i nghien cdu da chi r a rang cao cay Sucmg s i o cd tac d u n g hd trg dieu tri beo phi tren chugt n h i t t r i n g . Vdi lieu lugng tdi uu la 400 mg/kg can n i n g chupt giam 9,04 g cin nang hay g i i m 13,4% kh6i l u g n g c o the ban dau. N h d n g ket qua nghien eiiu cda chung t6i da chi ta tigm n i n g sd dyng cao ciy Suong s i o trong viec ho trg dieu trj beo phi, giup n i n g cao k h i nang u n g dung cay Suang sao trong linh v u c thuc pham chuc nang.
T A I L I E U T H A M K H A O 1. Ho Ba Vuong, Nguyin Xuin Duy, Nguyen Anh Tuin (2015), "Tii uu hoa tach chilt polyphenol tu la l i bing phuong phap be mgt dap irng", Tgp chi Khoa hpc vd Phdt triin, so 13 (7), tr 1144-1152.
2. Trin Thj Chi Mai (2007), Nghien ciiu tdc dung ciia polyphenol che xanh (Camellia sinensis) trin cdc chi so lipid vd trang thdi chong oxy hoa trong mdu chugt cong trang ddi thdo duang thuc nghiim, Luan an tiln sT Y hoc, Trudng Dai hgc Y Ha Ngi.
3. Vu Thi Ngoc Thanh, Nguyin Trgng Thong, Pham Thi Van Anh, Biii Thuy Duong (2005),
"Budc diu nghien ciiu tic dyng cua flavonoid chiet xuat td hoa kim ngan len mot s6 chi so lipid mau", Tgp chi Dupe hpc, so 1, tr. 15-18.
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S U M M A R Y
EFFECTS 0¥ MESONA CHINENSIS BET^TH. E X T R A C T ON O B E S I T Y T R E A T M E N T IN M I C E
Nguyen Hai Linh', Ma Dieu Quynh', Ma Thi Thu Le', Bui Thi Thu Thuy', Vu Thi Minh Ilong',Nguyen Thi Hong Hanh^' ' Vung cao Viet Bac high school. Thai Nguyen
Hanoi National University of Education Mesona chinensis Benth,, an important pharmacological effect plant, was planted popularly in the midland and northern mountainous region in Viet Nam, The aim of this study was to investigate the effects of Mesona chinensis Benth, in treatment obesity. Polyphenols and Flavonoids were quantified from Mesona chinensis Benth.extract and testing m 96 obese mice (Swiss) with oral doses ascending of 100-500 mg/kg body weight in 6 weeks. Polyphenols and Flavonoids levels in Mesona chinensis Benth. extract were 375 mg/g and 265,6 mg/g, respectively After 6 weeks, mice which dmnk Mesona chinensisBenth reduced body weight significantly. Oral doses of 400 mg/kg body weight had the highest reduction, decreased 9.04 g or 13.4% compared with body weight at the time before treatment (P < 0.05). Thus, Mesona chniensis Benth extract is effective on obesity treatment m mice.
Key words: Mesona chinensis Benth., obesity, mice. Polyphenols, Flavonoid
Ngdy nhgn bat: 24/02/2017; Ngdyphdn bien; 02/3/2017; Ngdy duyit ddng: 27/4/20J 7
