HOl NGHI KHOA HOC CONG N G H f SINH HOC T O A N Q U O C 2013
LUA CHON MOT S6 LOAl VI TAO GlAU DINH DU&NG CO KHA NANG SO"
DUNG T O T KHi CO2 TRONG DlfeU KIEN PHONG THi NGHIEM
Li Thj Thom\ Hoang Thj Lan An[)\ Dinh Thj Ngoc Mal\ Nguyen Cam Ha', Luwng Hong H^nh^, Dirong Tinjng Kien', Mai Van Quang', Trjnh Xuan Thanh', Nguyin Thj H^ng V i n ' , Ng6 Thj Ho^l Thu\ Oang Diim Hdng'', D^ng Dinh Klm^, Ph^m Bi>c Thuan^
' Viin Cfing ngh$ sinh hgc, Vien Hin lim Khoa hpc va Cbng nghg Vl$t Nam
^Vi$n Cdng ngh$ M6I tnrbng, Viin Hin ISm Khoa hQO vi Cbng ngh$ Vi$t Nam
^Vi$n Thifliphim chOvnang
TOM
T A TVi tao c6 kha nang sic dung inh sang mat troi va COj trong kiu quyln dS tdng hgp nSn hop chSt hiiu co cho co thS, g6p phan giam thieu lu^ng khi phat tti^ giy tu|u iing oM kinh, Idi^ kin chu trinh carbon tr^i dSL Day chinh ii co so khoa hgc cho vifc su: dvmg cgu^n khi thii CO2 tii ciic nbk miy, khSng gay SQC vbi ti bio tao d i niidi tr^ng vi tao giau dinh duong iam thuc pham chuc nang cho nguM vk dpng vSt nuSi, lam nguySn lifu san xu^t nhiSn li^u sinh hgc. DSy dugc xem ii c6ng nghf xanh, tlian thien vbi m6i tru6ng, gop phan giam t h i ^ khi phat thai nhi Unb, giam bi^n Sii khi hau toin cau v i l i m^t budng nghien cuu day tiran nang iing dyng b Vi^t Nam. Trong bai bao nay, chung toi xin trinh bay kSt qui lua ch^n dupc 2 {Nannochloropsis oculata HP va Spirulma platensis CMT) trong s6 5 loii {Dunaliella tertiolecta HP; Chlorella vulgaris HP; Bolrycoccus braunii; Nannochloropsis oculata HP. v i Spimlina platensis CUT) giau dinh duong c6 Jdii nang sii dyng tot COj cho sinh tnicmg Sinh truong ciia 2 loii tio N oculata vi S.
plalensis dSu d^t gia_trijao nliitklu syc COi 6 n6ng dg 2 va 5% (v/v) vdi t6c do syc 0,25 lit/ phflt Vi tio A^, oculata HP cfi m^t dg le bio circ dji dat 69,79 x 10* T§ bao/ml (TB/ml) v i 68,99 x 10* TB/ml Idii syc khi CO2 6 nbng dO 2 v i 5%, hrong 4ng. Trong khi di, loii S. plalensis c6 ODss6rm cao nhll d^t 0,7 viSi cic H^ng dg COi cung cSp 2 v i 5%, sau 7 ngiy nu6i c^y.
Tit khda: Syc khf CO2, vi tao, Nannochloropsis oculata HP, Spirulma platensis CMT MdDAU
Cic die diem sinh l;^, sinh hoa, thSnh ph4n dinh dip&ng vi c6ng ngh§ nu6i Ir^ng cQa m5t s6 Ioai vi t3o giau dinh diy&ng thugc cSc chi Chlorella, Spirullna, Dunaliella, Isachrysis, Nannochloropsis, Chaetoceros...6a dugc nght&n ci>u tuong floi kp 6-\fl^t Nam (D|ng Dilm HSng va cs., 2011), NgoSi ra, theo cfing b6 cCia m^t s6 tac giS nhu-Rahaman vS cs. (2011) thl cdc lodi vi tao khdc nhau c6 khS nSng hap thg lupng CO2 rat khdic nhau. Trong 66, Nannochlon^psis oculata cb khi nSng nufii trfing vfi'i nfing dfi COj ban dku tir 2-35% vi c& djnh tir 13-55% luwng C02 co du-g^ (Chiu et al-., 2009);
Bofrycocciis braunii tii 0,5-10% vi chua ro lu-gng CO2 c6 djnh du-gc (An e( al., 20O3); Chlorella vulgaris tir 2-15% vi 74%. tu'crng i>ng (Arief et al., 200g); Dunaliella tertiolecta ttr 2-10% va 60%, tuong iing; Spimlina platenls tir 4-6% vi 61,5%, luong i>ng (Watanabe, Hall 1996)
Carbon chi^m 40-50% sinh khfii tSo, do v$y d l sdn xull dugc 1.0 g sinh kh6i tSo t6i thilu cSn khoSng 1,6-2,0 g CO2 (Xianhai ef al., 2011). Theo cfing b6 cCia de Morals vk cs. (2007) sinh taring cila lio lam Spirullna platensis b nong dfi khi CO2 6 vi 12% cfi thl dat tdi da ta 0,44 va 0,22 g/l/ngdy, luong tfng vdi sinh khfil khfi dgt eye dai 0,35 g/l. Hal chCing vi tao lyc Chlorella sp. UK001 va C. littorals cfi khd nang co djnh 1 g C02^ngdy (Murakami et al.. 1997). nhung vo-i cac chung Chlorella sp. phan lap fi- su6i ufi'c nfing cfi thfi song a 42° C vfii vi nfing dg CO2 la 40% (Sakai et a/.,1995).
Yoo v^i cs. (2010) cung da cfing bfi kfii qua sang Igc dug'c dc loai Botrycoccus braunii, Chlorela vulgaris vd Scenedesmus sp. cfi khi nang sinh tru*ng tfit dufii dilu W&n C02 din 10%, Khi do, sinh khfii tao va hdm luong lipit cCia Scenedesmus sp. dat 217.50 vd 20,65 mg/l ngdy (chifim 9% sinh khfii) cfin doi vol B. braunii Id 26,55 va 5,51 mg/l ngdy (chiem 21 % "sinh khfii). Trong cfing trinh cua ldc gia Sydney vd cs. (2010) da cho thay khd nang cfi d[nh CO2 cao nhit b lodl e. braunii, Bfip sau la Splninna platensis. Dunaliella tertiolecta, Chlorella vulgans dgt lln iugt Id 496,98; 318,61;
272,4 Vd 251,64 mg/i ngdy.
Dya tr6n cdc ket qud nghien ci>u cila cdc tdc gia tren thfi gifi'i da cfing bo ve kha nang sir dyng CO2, khi ndng luong thfch vfi cflng nghg nufii trfing tren quy mfi lo'n vd djnh hufi-ng Ong dyng lam thyc phfim chijc nang, thufic chCra b$nh, Idm fliil-c an trong nufii trfing thuy sdn, idm nguon nguygn lieu cho sin xuat nhi§n lifu sinh hgc... chiing tfil da tiin hdnh sdng Igc trong dieu kign phfing thi nghi|m cac chilng/lodi tao da sin cfi nham chon ra dugc dc chung/lodi tao cfi kha ndng nufli trfing tren quy mfl Ifi-n cflng nghigp. cfi khd nSng cfi d[nh CO2 cao, cfi thSnh'phan dinh duong tfit vd la dfii tupng Imyfin thfing duoc sit dyng Idm fliu-c phim chiic ning cho ngufi'i vd dfing vit nufli...
VjfliT L1$U VA PHlfaNG PHAP Chiing gifing va moi tru>6>ng nuol
Lodi vl tdo lam Spimlina platensis (Gomont) Geither CNT vd lodi Nannochloropsis oculata (Droop) Hibberd 1981 HP, Chlorella vulgaris Beijerinck 1899 HP, Dunaliella tertiolecta Butcher 1959 HP, Botrycoccus braunii Kutzing do phfing Cflng ngh$ Tdo. Vien Cfing ngh# Sinh hgc, Vi^n Hdn lam Khoa hgc vd Cfing nghS Vi#l Nam phSn Igp va luu giO' du-pc su dyng trong nghien a>u (0|ng Di§m Hong vd cs., 2011).
Cdc loditio dupc nufii trong mfli tnibng thich hpp nhu: mfii Irufi'ng SOT doi vdi S. platensls. Walne cho W. oculata, C.
vulgans vi D. tertiolecta; mfli truing BG-11 cho fi. braunii.
Phuwng phap
Thf nghidm lira chon mgt so lodi tao co kha nang si> dung kh! COz
. Thl nghl§m dupc tifin hdnh trong cac blnh tam gidc 500 ml cfl "chi>a 300 ml mfii tnrimg; chiiu dnh sang cfi cu&ng dO la
3,5 klux vfl'i quang chu kJ sdngitfii ld_ 12:12 gi&; sinh Irufi'ng cua tao dupc xac djnh qua thflng sfi ODssa^^ dfii vbi S.
H O I N G H ! K H O A HCpC C ( ! ) N G N G H E S I N H H O C T O A N Q U 6 C 2 0 1 3
platensls vd ODesftm cho cac loai lao cfin Igi; OD ban d l u cho » i i nghifem dbi voi cacloai tao Id O^STT'^'tian dhu t h i "
nghigm Id 9 cho S. platensis vi 7,2 cho c i c l o i i tao cfin lai; sgc khi C 0 2 5% (v/v); thi nghiem d u p c theo dfli trong vong 15 n g i y , moi cflng thCrc thf nghifim d u p c l | p Igi 2 ihn. Cb sau 5 n g i y lay m l u xac djnh sinh t o r o n g cua l i o , hdm lupng hydratcacbon, phycocyanln theo cflng bfi cCia D | n g D i l m Hfing va cs. (2011), xdc dinh ham l u p n g lipit theo phuong p h i p cOa Bligh vd Dyer (1959).
Phu'cmg phap xdc d j n h p h y c o c y a n l n e , c h l o r o p h y l l a, c a r o t e n o i f cQa t a o Spirullna platensis
Mfit lupng tao b i l l trtpoc d u p c nghifin v 6 i cdt IhOy tinh trong a | m phosphate 0,01 M, pH 7 trong Ignh rfii ly tSm 8000 v/p trong 10 phut. Thu p h i n djch mdu lam b trfin cfl chi>a phycocyanlne. Chiet n h u vgy 3 lan den khi djch ohifil hfit m i u lam rfii djnh m i i c ISn 50 ml bang dung djch dfim IrSn vd do pho hfip thy a bubc song 622 nm d l xdc djnh hdm l u p n g phycocyanln, -
P h i n c i n d u p c hfla tan v i o 20 ml aceton 80%, ly tdm 8000 v/p trong 10 phut. Lap Iai qud Irlnh tren 2 iSn. Djch thu chCra chlorophyll a va carotenoil dirpc djnh mi>c Ifin 50 ml bang acetone 80% vd do OD tai bubc sflng 663 nm dfi x i c t^nh hdm lirpng chlorophyll a v i Igl 460 nm dfi xdc djnh carotenoit
Hdm l u p n g sac to tinh theo cflng thi>c:
C = (OD X 50 X1000) / (5 X m x V) C = nra/g
50: the tich nang Ifin bang dfirn hay dung mfli (ml) V: t h l tlch djch teo dOng d l xdc djnh s i c tfi (ml)
' M: khfii l u p n g khfi ldo t u p n g i>ng vfi'i V(ml) djch l i o dem frfian tich (g) -SphyccKyanlna ^ '
^ d i l a n > p h ) l B = 8 2 , 0 4
^ c a n ) ^ = 2 0 0 .
"nil n g h l ^ d n h h u ^ m g cDa n o n g db khf C O : tinh k h i e t khac n h a u len s i n h tru'fl'ng ciia vi t d o bien N. oculata vi tao tam Spimlina platensls
N. oculata HP vd S. platensls CNT dirpc thf nghi$m trong c i c binh tam gidc 500 ml cfi chiia 300 ml mfii trufl'ng t u o n g i>ng. c h i l u i n h s i n g cfl cufl'ng dg 3,5 klux vfi'i quang chu kjr sdng: toi Id 12-12 gifl'; m i t d$ ban dfiu cho thl nghi$m Id 7,52 xlO^ TB/ml (vfi'i N. oculata) v i ODsssnm niim trong k h o i n g 0,3-0,4 (vfi'i S. platensis CNT). CO2 finh k h i i t cfi nfing dfi to 1. 2.^5.10 vd 15% (v/v) d u p c dOng cho thl nghifim dfi lim ra difiu ki^n syc CO2 toi u u . Cdch tgo ra nfing dfi CO2 khdc nhau bang each t i l n h i n h trfin l§n khi CO2 tinh khiet > 9 5 % vfi'i khflng khi, phan tram the tlch cue COz da qua filter lgc cfl kfch thufi'c io l i 0.22 pm de tgo ra d u p c cdc nfing dfi CO2 Id 1 . 2 . 5 , 1 0 va 15% (v/v). Khi C02,dupc syc vfi'i tfic dfi 0,25 lll/phiit, n h i f t dg nuoi d u p c duy 6 28 ± 0,5° C. Theo doi thf nghigm trong 7 ngay. Mfii cfing thiic t h / nghifim d u p c l | p Igl 3 ifin. Hdng ngdy lay mau x i c djnh pH bang gifiy quJ vd mdy do pH, x i c ^ n h tfic dfl sinh W o ' n g thflng qua mfit dfi tfi bdo (vfl'l l i o N. oculata) v i ODssp™ (vfl'i t i o S, platensis CNT) theo cflng bfl cCia Dgng Diem Hong vd cs. (2011).
Cdc sfi ligu d u p c xi> I;^ thfing kfi bang phfin m i m Excel vd thfing ke ANOVA mgt thdnh p h i n .
K ^ T Q U A V A T H A O LUAN
Thf rtghlfim lira c h g n m f l t s f i l o i i t d o c f i k h d n3ng si> d u n g khf COz
Dfi nghien c u u ve nong dfi CO2 tfii i n j cho sinh tnrfrng ci3a 5 lodi vi tao, chiing 161 da t i l n hdnh nufii c l y cdc lodi t i o nfiu trfin tnang mfii tnrbng h^ong l i n g cfl syc khf CO2 5% (v/v), arfi'ng dg dnh sdng la 3,5 klux vfi'i quang chu ky s i n g : ifii l i 12:12 gifi'. Kfit qua nghien c i i u d u p c ch? ra b hlnh 1 .
Hlnh1. Sinh tnj'd'ng cCiaS l o l l vl t i o trong moi truong nuoi c6 sgc 5% {vht) khf COi
Kfit qud nghien c i m d u p c Irlnh b i y trfin hlnh 1 cho t h i y sau 15 n g i y nuoi cfiy. sinh truong cua lodi S. platensls Id tfit n h i t , sau d i n N. oculata, C. vulgaris, D. tertiolecta vi cu6i ciing la Braunii t u o n g Crng vfi'i ODsssfim Id 1,21; O D M O ™ Id 0,92; 0.69; 0;55 vd 0,53. N h u v | y , b nfing dfi khi CO2 Id 5% (v/v), sinh trucmg ciJa 2 l o i i S. platensis v i N. oculata Id
•cao nhfit. Ngoai ra. theo cfing bfi cua t i c gid Elzenga vd Prins (2000), 2 loai vi tao neu trfin co d^c d i l m k h i c nhau v l k h i ndng sit dyng C02 (Bieo cdc con duirng k h i c nhau) d y a vdo hogt dflng cfla enzym carbon anhydrase (CA) ngogi bdo N. oculata - v i tao b i l n , khfing cfi CA, si> dyng tn,rc tifip H C O j ' va S. platensls - vl tao lam n u d e nggt - cfi CA, si>
dyng COi^.
Sau 15 ngdy thf nghi?m, chiing tfii Hfin h i n h thu sinh Wifii ^ o va phdn tfch pnatein, hydralcabon vd lipit cOa 5 lodi w t i o nfiu trfin. Kfit qua nghifin ci>u dugc chi ra trfin hlnh 2 vd 3.
HOI NGH! KHOA HQC C O N G NGHE SINH HQC T O A N Q U ( 5 C 2013
Theo Paulin v i cs. (2006) cfing bo, cdc l o i i vi lao khac nhau cfi thdnh phfin dmh d u o n g khac nhau. Trong dfl.^thdnh phan prof&in. hydratcacbon vd lipit cua C. vulgaris Id 51-58 picogram/l§ b i o (pg/TB), 12-17% v i 14-22% sinh khoi khfi, tucng ung. D. tertiolecta - 57 pg/TB, 3 2 % vd 6% sinh khfii kho, t u o n g irng. S platensls - 60-71 pg/TB. 13-16% va 6-7%
sinh khfii khfi, tucmg ung. Nufli trfing N. oculata trong difiu kign syc khi COz 5% (v/v) OD hdm luang lipit Id 2 6 , 2 % sinh khfii khfl (Chiu et al., 2009). K i t qua nghien c i i u thu d u p c tren hlnh 2 vd 3 da cho t h i y trong deu kign thl n g h i f m Vifit Nam, Bidnh dinh dirong ciia tdo d u p c nufli trfing trong d i i u kifin syc khf CO2 5% (v/v) Id hoan l o i n phO h p p v d i cdc cflng bfi nfiu trfin. Trong d o , S. p/a/ens/s CO hdm l u p n g protein, hydralcabon vd lipit dat d u o c i f i n l u p t Id 41,52 pg/TB; 10,78%
vd 12,7a?!,-sinh khfii khfl; N. oculata - 8,29 pg/TB, 34,72% va 18,72% sinh khfil" kho, t u a n g Cmg. D. ter^olecta - 28,94 pg/TB, 14,15% vd 10.79% sinh khfii khfl. t u a n g Crng; C. vulgaris - 39,85 pg/TB, 17,17% vd 9,12% sinh khfii khfl. tuo'ng l i n g ; B. braunii - 39.12 pg/TB. 3,49%, 34.92% sinh khfii khfl, t u c n g Crng, Ket q u i nghifin c u u (^ cho phep l y a chpn 2 lodi S. platensis vd N.oculata cho nghifin cii'Li t i l p theo.
Hlnh 2. Thinh p h i n protein cda 5 lodl tdo trong moi t r u i n g c6 syc CO] tlnh khief 5% (v/v)
Hinh 3. Thanh phan hydratcacbon v i llpIt cua 5 lodi tdo trong mfli favd'ng cfl syc COj tlnh khiSt
5% IvM
T h l nghidm dnh hirflmg n o n g d g k h i CO2 t l n h khiet khdc nhau Ifin sfnh tnj'd'ng ciia v i tdo bien N. oculata v a 5 . platensis
Sau khi lya chgn d u p c 2 lodi cfi khd ndng si> dung tfit C02cho sinh trufl'ng, chiing tfii tifin hdnh nghien cCru dieu k i f n syc khi CO2 tfii u u ISn sinh t r u o n g ciia chiing. Khi sgc CO2 cfi nfing dfi khac nhau sfi Idm thay dfii pH mfli trufl'ng nufii khdc nhau. K i t q u i do pH d u p c trinh bdy fi' hinh 4 cho thay dfii vfl'l N. oculata, syc khf CO2 vcri nfing dg c i n g cao thi pH mfii Inffl'ng nufli c i n g g i i m do COz phdn Crng vfl'i nufl'C tgo ra ion H* v i HCOa" theo p h u o n g trinh: CO2 + H2O = H2CO3 vd H2C03 = H* + HCO3' lam pH mfii Irufi'ng gidm xufing. Syc C O ; if'dc nfing dg k h i c nhau: 0,03, 1 ; 2; 5; 10 va 1 5 % (v/v) nhung gia tri pH dupc duy tri 6' mCrc trung blnh 7,98; 7,94; 7,80; 7,72; 7 , 3 3 - v i 7,02, t u o n g i j n g . Kel q u i nay p h i i hpp vdi cflng b l ciia Chiu vd cs. (2009), khi syc CO2 6' nfing dfi 2, 5, 10 vd 15% (v/v) vao mfli tnrfi'ng nufii thl tdo N. • oculata cfi pH mfii tnrfi'ng lufin d u p c duy tri fl- mCrc 7.8; 7.7; 7,3 vd 7,0 l u o n g i i n g .
Hinh 4. Anh hirong cCia nong da khf CO2 tinh khl4t khdc nhau len pH moi hirong nuSl N. oculata
Hlnh 5. Anh hud'ng cua nong dg khi CO2 tinh khiet -khac nhau ldn pHmdltrVOTig nuol S.pfatensjs Trong khi dfi, dfii vcri S. platensis, pH mfii tiU'O'ng nufli cfi b i i n dfing trong ngdy va sau 2 4 giP syc khf CO2 l i khdc n h a u . Treing 1 gifi' sau syc khi COz, pH mfii tru&ng nufli cfi x'u hufl'ng gidm d i n . Nhung khodng 5-7 gifi' sau syc khf COz.-pH mfii tnffl-ng nufli Igi phyc hoi vd sau 24 g i o , pH sfi dgt gid trj x I p xf vd cfl cao hon mflt chiit so vfi'i gid trj pH ciJa ngSy hflni tnrfl'c. D i l u ndy cho thay, khi h i m l u p n g khf COz tang ti> 0,03% (khflng khi) dfin 15% (v/v) thl pH mfii trubng.nuQi trfing S. platensis tang l&n so vfl'i cfing thi>c dfii chung.
n g i NGHI KHUA HOt-i CQl^lGTlSHE SINH HOC TOAN QUOC 2013
K l l j ^ u i ngh[6aci>u d u o c trinh bay-fi'-hinh.6 cho thay.^irihJrufi'ng cOa W,-ocu/afa-dat-gid tri cao nhlt-o'-cfing-fhiVr-nn--
"^6rig"a9'COrl3^d"5%TWi7[T?&TtS&^6 dflng khi la 0,25 lit/ p h u t Mgt dg te bao dgt dupc Id 68.79 v i 6 8 . 9 9 x 1 0 ^ TB/ml, t u w i g i>ng, sau 7 ngdy nufli cay. K i t qua thu d u p c fl' d i y h o i n todn phO hop vfi'i cflng bfl cOa l i c g i i Chiu vd cs, (2009) (sau 8 ngdy nufii c l y trong dieu ki#n nfing dfl COz 2 % (vAr), tdo co mdt dfi te bdo dat 69.39 x 10^TB/ml). K i t q u i nghien ci>u thu d u p c trfin hinh 7 cho t h i y sinh trufl'ng cua S, platensis dgt cao n h l l a nfing dfi CO2 Id 2 vd 5% (v/v) v d i ODssenm dfii!i dgt 0.7 sau 7 ngdy nufli c l y ; tfic dfi sinh tnffl'ng dgc tarng cfjrig dat cao nhat Id 0^172 vd 0.167/ngdy. t u o n g Crng.- K i t -• q u i thu d u p c nSu tren h o i n t o i r l phCf'h'pp''V&i cflng bfl cOa t i c gia Da Rosa v i cs. (2011) khi nufli S. p/a(ens/s 6 bfi h d
cfi syc COz 6%. tfic dfi smh tru&ng d i e trung dgt 0,12/ngiy.
Hinh 7. Anh hu'cmg cua nong dg khi C d tinh k h i l t khdc nhau len sinh tnrd'ng c£ia S. platensls Hlnh 6. Anh h u ^ g ciJa nbng d$ khf CO] tlnh khiet
~- khac nhau l l n sinh truong cGa IV. oculata
K f i T LUAN
TCr c^c k i t qud nghifin cCru thu d u p c d u p c Irlnh b i y b tren, chCing tfli nit ra mfit sfi k i t lufin sau:
Trong d i l u klfirt phfing thl nghifim da l y a chgn d u p c 2 lodi N. oculata HP vd S. platensis cfl k h i n i n g sinh irufi'ng tfit nhfil'khi nufli trfing cfl si> dgng 5% (v/v) CO2 trong 5 lodi vi tao S. platensls, N. oculata HP, Dunaliella tertiolecta, Chlorella vulgaris, Botrycoccus braunii.
Sinh.tnrfl'ng cda 2 l o i i t i o N. oculata vd S. plalensis deu dgt gid tri oao n h l l khi sue CO2 fi' nfing dfi 2 vd 5% (v/v) vfl'i tfic dfi sgc Id 0,25 lit/ phdt. VI tao N. oculata HP-co m | t dfi t l bao e y e dgi dgt 69,79 x 10^ TB/ml vd 68.99 x 10^ TB/ml khi syc khf CO2 b nfing dfi 2 vd 5% (v/v), t u o n g Ci-ng. Trong khi dfl, lodi S. plalensis cb ODjsBnm cao n h l l dgt 0,7 vfl'i cdc nfing dfi C02 2 vd 5% (v/v), sau 7 ngdy nufli e l y . Tuy nhifin, d l t i l l kifim khi CO2, chiing tfli da l y a chpn nfing dfi 2 % (v/v) dfii v d i c i c thl nghifim khi sO dyng k h i C O ; tinh khiet vd efi t h l Id 5% (v/v) d l i vfi'i trufl'ng hpp khi si/ dyng C O j tCi' 16 dfit than d l t i l n h i n h c i c thl nghigm liep theo.
L&i c ^ ffn
Cong'trink da dirpc ko tr^ Idnh phi tit de tai "Nghien cuu cong ngh^ Ihu hoi CO2 tir khi thai dot than de sdn xuat sinh khSivi tdo gidu dinh duang''ma so KC08.08/ll-,JS thupc Chuong trinh "Nghien cuu-ldiocliqccougngh^phifCyupIiohgifdnh ihien tai, baov?
moi trudng vd sii d\ing hop ly tdi nguyen thien nhien" do GS. TS. Bang Binh Kim. Vi$n Cong nghf mdi trudng, Vi^nHdn l&m Kkoa hpcva Cong ngh? Vift Nam ldm chu nhiem vd "Nghien am xdy difng quy trinh cong nghe nudi vi Ida Nannochloropsis oculata bdng thiet bi Photo-Bioreactor de son xuSt thifc phim chitc nang" do ThS. Phgm Buc Thit^n ldm chii idii^m thu$c sd khoa hpc vd cdng nghf Hd Not nam 20]2'2013 cap kinhphi.
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SELECTION OF SOME NUTRIENT - RICH MICROALGAL SPECIES CAPABLE OF EFFICIENTLYULTILZING Cpz UNDER LABORATORYCONDITIONS
Le T h i T h o m \ Hoatig Thl Lan A n h \ D i n h T h l Ngoc M a i \ Nguyen Cam H a \ L u o n g H o n g H a n h ' , D u o n g T r u n g K I e n \ Mai Van Q u a n g \ Trinh X u a n T h a n h \ Nguyen T h i Hong Van'', Ngo Thi Hoai Thu , Dang Diem H o n g ' ' , D a n g Dlrih Klm^, P h a m Due Thuan^
^Institute'of Biotechnology, Vietnam Academy of Science and Technology
^Institute of Environment Technology, Vietnam Academy of Science and Technology
^Institute of Functional Food ^ • —
SUMMARY
Microaigae have the ability to fix CO^ using solar energy to produce biomass CO^ fixation by photoautotrophic algal cultures h a s flie potential to reduce greenhouse gas emissions, helping alleviate the trend toward global warmmg and closing the Earth's carbon cycle.
The biological capture of CO2 from Que, gaS usmg microaigae has the following advantages: (i) non-toxic for microalgal growtti; (ii) being an environmental sustamable method and ^iii)cO'producing liigh added value materials based on biomass, such as human food, animal feed, bio-eneigy. Ttiis woik aimed to select nutrient-rich microaigae species for CO2 sequestration systems. Among those tested, two of five screened microalgal species, Nannochloropsis oculata HP and Spimlina platensis CNT, were tlie best in terms of the growth rate and CO2 capture. These sb-ains showed the highest growtli rate at CO2 concentrations of 2 % and 5 % (v/v) w i t h flow rate of 0.25 L/min. N. oculata HP reached the highest cell density of 67.79 x 10' cells/mL and 68.99 x 10* cells/mL a t CO2 concentration of 2 % and 5% (v/v), respectively. Meanwhile, the highest ODsssnm value of 0.7 was obtained at COj concentration of
"2% and 5% (v/v) after 7 days of cultivation for 5. platensis.
Keywords: COj aeration, Microaigae, Nannochloropsis oculata HP, Spimlina platensis CNT lAulhor for conespondence: Tel.+84,3.7911059, E-mail [email protected]
