recipe BT (from Quang Ninh) and TL (from Tuyen Quang) exhibited hypoglycemic effects on both nornoglycemic mice and streptozocine-lnduced hypoglycemic mice. Meanwhile, the recipe GI (from Cao Bang) had no impacts on normoglycemic mice but inhibited hyperglycemic action of streptozocine in mice. These findings partly proved the efficacy of these three ethnic recipes for treatment of diabetes melitus by minority communities for years.
Key words: traditional recipe, hypoglycemic activity.
Tai lieu tham khao
1. Bd Trung Dam, Phuong phap ngoai suy liiu c6 hieu qua tuong duong giua nguai va dpng vgt thi nghiem, T^p chi Dugc hge (2001) so 2, tr 7-9.
2. Nguyen Khanh Hoa, Dao Van Phan, NguySn Duy Thuan, Ostenson C. G. , Tac dung ha duang huyet cua that di#p ddm tren dpng vat thuc nghiem.
Tap chiy hge thuc hanh (2005), 37(4), tr 10-13.
3. Govindarajan R et al. Effect of Desmodium gangeticum extract on blood glucose in rats and on insulin secretion in vitro, Planta Med (2007), 73(5), 427-432.
4. Megalli S, Davies NM, Roufogalis BD, Anti- hyperlipidemic and hypoglycemic effects of Gynostemma pentaphyllum in the Zucker fatty rat, J Phamn Phanv Sci (2006), 9(3), 281-191.
5. Norberg A, Hoa NK, Liepinsh E, Van Phan D, Thuan ND, Jurnvall H, Sillard R, Ostenson CG , A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum, J Bio Chem (2004), 279(40), 41361-41367.
6. Umamaheswari S, Mainzen Prince PS , Antihyperglycaemic effect of 'llogen-Excel', an ayurvedic herbal formulation in streptozotocin- induced diabetes mellitus. Acta Pol Phanv (2007), 64(1), 53-61.
7. World Diabetes foundation. The global burden of diabetes. Diabetes Atlas second executive summary (2003), 7-11.
Che bien hac phu, bach phu va bao che cao l^ho phu tir fr quy mo pilot
Dat van de
Phu tir thu tu edy 6 diu Sa Pa {Aconitum carmichaelii Debx. var. carmichaelii) da duge nghien euu ehi biin va tdc dung sinh hpc '^'"'l Cdc tdc gia da chpn dugc phugng phap ehi biin phu tu' vd bdo ehi eao phu tii' chiit nude d quy md thi nghiem eho cac san phim co doe tinh thip vd co tdc dung eudng tim tren tim ich co lap, tim thd eo lap. Bai bdo nay trinh bay kit qua nghien euu ehi biin hie phu, bach phu vd bdo ehi eao kh6 phu tu d quy m6 pilot, ham lugng alealoid todn phin, diester'alealoid, aeonitin trong eae miu nghien cuu va ddnh gia doe tinh elp, tdc dung tren tim vd mach vanh thd c6 lap eiia eao hie phu, eao bach phu vd eao kh6 phu tu.
D 6 i tu'O'ng va Phu'O'ng phap 061 tu'gng nghien cii'u
Phu tir {Radix Aconiti carmichaelii lateralis) thu tu edy 6 dau trdng g Sa Pa vdo thgi ky cay dang ra hoa (thang 9/2007 vd thang 9/2008).
Bui Hong Cucmg, Phung Hoa Binh Trudng dgi hoc Dugc Hd Noi
Dong vat thi nghiem
Thd ehung Newzealand White, 16ng trdng, trpng lugng 1,8 - 2,5 kg do Trung tdm chan nuoi - Vien Kiem nghiem cung cip. Dpng vdt duge nudi trong diiu kien diy dii thuc dn vd nude udng tai phong thi nghiem, Khoa Dugc ly - Sinh hod, Vien Dugc lieu tu 3 - 7 ngdy trudc khi nghien cuu vd trong sudt thgi gian nghien cuu.
Phu'O'ng phap nghien cii'u
Che bien hac phu, bach phu, bao che cao kho phu tw
Chi biin hie phu: Phu tu' sdng (tugi) rira sach, loai tap, ngdm vdi dung djeh magnesi clorid, dun sdi din chin, vdt ra ru'a sach, thdi phiin dpc cu ddy 3-5mm, ngdm rua trong nude, tam djeh dudng dd vd diu hat cai. Hip hai nude soi din khi b i mat phiin bdng, mdu ndu vdng. s l y kho.
Chi biin bach phu: Chpn cu loai nhd, cdt bd
r i con, ru'a sach, ngdm phu tu vol dung djch
MgCb vdt ra rua sach. Lupe vdi nude d i n khi chin hoan toan, bdc bd vd den, thai phiin dpc eii ddy 2-3mm. Ngam ru'a phiin phu tu blng nude. Dd h i p chin (khoang 30 phiit). Phai bode s l y d i n kho kiet
Bao chi cao khd phu tu: Phu tu sdng ru'a saeh, thai phiin, s l y khd. Chiit x u i t blng nude s6i, CO d i n cao ddc, s l y khd thu dugc eao khd phu tir.
Dmh tinh alealoid
Djnh tinh alealoid bang cic thudc thu (TT) Chung: TT Dragendorff, TT Bouehardat, TT Mayer.
Djnh two'ng alealoid
Djnh lugng alealoid toan phin: Phuang phdp acid - baze '^'
Djnh lugng diester alealoid: Phuang phap do dp h i p thu tir ngoai - kha kiin '^' ^'. Do tren may quang phd tir ngoai - kha kiin U-2010 (Hitachi) - Nhat Ban tai phdng Kiem tra c h i t lugng, Cdng ty ed phin Traphaeo. Chit chuin: Aeonitin eua hang Latoxan - Phdp.
Djnh lugng aeonitin: Phuang phdp sde ky Idng hieu ndng cao (HPLC) '^'. Trien khai tren may s l e ky ldng hieu ndng eao (HPLC) Merk- Hitachi D7000 tai phdng Kiem tra ehit lugng, COng ty ed phin Traphaeo. Chit chuin:
Aeonitin cua hang Latoxan - Phdp.
Nghien cu'u tac dung sinh hoc Miu nghien cwu
- Hac phu, bach phu dugc bdo c h i dang eao kho: Nlu 3 lin vol nude, thdi gian soi mdi lin 1 gid. Gop eae djeh chiit ed d i n dang eao Idng 1:1.
Qe ling loai tap, gan djeh trong, c6 edeh thuy d i n dae, sly khd, xay bpt, khi thu hod tan lai blng nude d i n the chit thich hgp thu dugc ede dung djch gdc de thir tae dung sinh hpc.
- Cao khd phu tu: Hod tan cao khd phu tu vdo nude vua dCi d i n t h i ehit thieh hgp, thu duge dung djeh gdc d i thu' tdc dung sinh hpc.
Thw tac dung tren tim co lap: Tim thd dugc c6 lap theo phuang phdp Langendorff vd dugc truyin dung djeh Ringer - Locke d i nudi tim.
Pha m i u nghien cuu sau khi da Ipc loai tap vdo djch nudi tim vdi ndng dp thieh hgp vd truyin vdo dpng mach vdnh thd vdi tdc dp blng 1/10 tdc dp dung djeh nudi ehay qua tim, Theo doi t i n sd dap eiia tim, bien dp eo cua tim va luu lugng mach vdnh thdng qua the tieh dung djch chay ra qua tim trong 5 phut trudc vd sau khi
truyen cdc dung djch m i u nghien euu tren mdy ghi da nang cua hang Ugo Basile, Italy.
XLP ly s6 lieu bdng phuang phdp thdng ke y sinh hpc, k i t qua thi nghiem dugc biiu thj blng trj sd trung binh cpng/tru sai sd ehuin ( x ± 5 £ ) .
K§t qua nghien
CLPUC h i biln hdc phu, bach phu, bao c h i cao kho phu tip
Che bien hac phu
- Cong thire c h i b i i n : Phu tir tuai dugc rira sach, ngdm trong dung djeh MgCl2 dam dae theo eong thire:
Phu tir tuoi 150 kg MgCl2.6H20 60 kg Nude 30 lit
- Thgi gian ngam: Mdi ngay k i i m tra mue dp n g i m cua dung djch MgCb vdo cii phu tir 1 l i n . Sau 4 ngdy, dung djch MgCl2 n g i m d i u todn bp cii phu tu.
- Dun sol vdi djch ngdm d i n khi phu tii' chin m i m tdi loi (30 phut). Vdt ra rira.
- Thdi dpc cu thdnh phiin ddy 3-5 mm. Ngdm phien phu tu trong 300 lit nude (ngap duge lieu) trong 12-14 gid, rua lai 3 l i n b l n g nude. De rao, s i y nhe (d 55 - 60°C) d i n kho se.
- T i m phu lieu: T i m phiin phu tu vdi dich dudng dd (3 kg dudng dd hod vdi 3 lit nude ndng), d i u hat eai vua du t h i m d i u be mat phiin (1,5 kg d i u hat cai).
- 0 12 gid. H i p hai nude sdi 20 phiit.
- S l y d 60°C d i n khd (dp i m 9 - 11 %).
-Ddng gdi kin, bdo quan nai kho, mdt Hieu s u i t e h i b i i n : 42,8%
Hdc phu phien vd ngodi nau den, mdt cat c6 mdu vdng s i m , nhuan bong, due md, the c h i t cung, chle, miii tham nhe, vj cay te nhe.
Che bien bach phu
- Cdng thuc c h i bien: Phu tir tuai dugc rira sach, ngdm trong dung dich MgCl2 ddm ddc theo cong thue:
Phu tir tuai 150 kg MgCl2.6H20 60 kg Nude 30 lit
- Thdi gian ngdm: Moi ngdy kiem tra muc dp n g i m ciia dung djch MgCb vdo cu phu tu 1 l l n . Sau 4 ngay, dung djch MgCl2 n g i m deu todn bp eii phu tu.
- Dun soi vdi djch ngdm den khi phu tir chin m i m tdi loi (30 phiit).
TAP CHI DUQC HQC - 8/2010 (SO 412 NAM 50) 33
- Vdt ra rua, boe bd vd,
- Thai dpc eu thanh phien day 2-3 mm.
- Ngam phiin phu tu trong 300 lit nude (ngap duge lieu) trong 12-14 gid,
- Rira lai 3 l i n blng nude.
- Qe rao, s l y nhe (d 55 - 60°C) d i n khd se.
- H i p hai nude s6i 20 phiit.
- S i y d 60°C d i n khd (dp i m 9 - 11 %).
-Dong goi kin, bao quan nai kho, mat, Hieu s u i t e h i biin: 37,2%,
Bach phy co mau trlng nga, the ehit eung, chle, mili tham nhe, vj cay te nhe,
Bao che cao kho phu tw (chiet nwac) - Chuin bj nguyen lieu: Phu tu' sdng, rua saeh, dd m i m (60 phiit), thai phiin day 2-3 mm, sly 60°C d i n khd (dp I m 9-11 %) thu duge phu tu phiin (PTP), Hieu suit ehi biin phu tu phiin dat 83,13%,
- Quy trinh bao c h i eao kho phu tu (chiit nude):
+ C5ng thirc n i u eao:
Phu tir phiin 50 kg Nude 950 lit + Quy trinh ehiit x u l t chiit 4 l i n :
L i n 1: n i u 50 kg -phu tir phiin vdi 300 lit nude, N l u s6i 4 gid, riit djeh chiit 1,
L l n 2: bd sung 250 lit nude n l u s6i 2 gid, riit
^diehehiit2,
L i n 3: bd sung 200 lit nude n l u soi 2 gid, rut djch ehiit 3,
L i n 4: bd sung 200 lit nude n l u sdi 2 gid, riit djeh chiit 4.
Tdng thai gian soi: 10 gig
+ Co dieh ehiit d i n dang eao dae: Gop cac djch chiit 1, 2, 3, 4, d i ling qua dem (12-14 gig), g ngi mat Gan liy djch trong, Ipe qua 1 Idp vai. Co djeh ehiit d i n dang eao ldng 1:1, tiip tue co each thuy d i n dang cao dac. Tdng thdi gian co: 24 gid.
+ S l y kho: Cho cao kh6 phu tir ra khay, s l y 70°C-80°C d i n khd (dp i m 4,5%) duge eao khd phu tu, Dong goi kin, bao quan nai kh6, mat
Cao kh6 phu tu thu duge eo t h i ehit khd, Bang 1: Ham lugng alealoid toan phin, diester phu va cao khd phu tu
kh6ng dinh tay, mdu ndu den ddng n h l t vj ding, khdng edn vj te, mui hai hie,
Hieu suit bdo e h i : 20,4 % Dmh tinh alealoid
Dinh tinh bing thuoe thw chung cua alealoid
L i y khoang 2 g bpt phu t u sdng, h i e phu, bach phu hoae 0,4g bpt eao kho phu t u cho vdo binh non dung tich 50 ml cd niit mdi, t h i m I m blng amoniac dae, Sau 10 phut them 20 ml ether ethylic (TT), l i e d i u , niit kin vd d i yen 30 phiit, thinh thoang lie, Gan l l y Idp ether, Idm khan blng natri sulfat khan, Ipe, bdc hai djeh Ipe tren each thuy tdi khd duge e i n , Hoa tan ein thu duge b l n g 5 ml dung djch H2SO4 10%, cho vao 3 dng nghiem, Djnh tinh b l n g TT Mayer, TT Bouehardat vd TT Dragendorff.
Kit qua djnh tinh alealoid bing phan irng hoa hoc cho thiy. cac m i u nghien euu d i u eho phan irng d u g n g tinh vdi 3 thude thu ehung eua alealoid.
Dinh lu'ang alealoid
Djnh lugng alealoid toan p h i n , diester alealoid, aeonitin trong cac m i u phu tu sdng, h i e phu, bach phu vd cao kh6 phu tu:
Can ehinh xdc khoang 10 g bpt dugc lieu hoac lugng cao kh6 phu tir tuang duang khoang 10 g duge lieu, ehiit xuit vd djnh lugng alealoid todn phin theo phuong phap DDTQ 2005 '^'
Can chinh xdc khoang 5g bpt phu t u sdng, lOg bpt hdc phu, bach phu hoac lugng cao tuang duang vdi lOg dugc lieu chiit xuit va djnh lugng diester alealoid theo phuang phdp DDTQ 2005'^'^'
Cdn ehinh xde khoang 5g bpt phu t u sdng, lOg bpt h i e phu, bach phu hoac lugng eao tugng duang vdi lOg duge lieu chiit x u i t va djnh lugng aeonitin theo phuang phdp duge neu trong tdi lieu '^'
K i t qua djnh lugng duge trinh bdy a Bang 1 alealoid, aeonitin trong phij tu sdng, hac phu, bach
Miu nghien ciru Phu tip sdng
Mac phu Bach phu Cao kho phu tir Cao kho phu tir (tinh theo duoc lieu)
So mau 15
9 9 9 9
(n) Ham lugng alealoid toan phan (%)
0,87 ± 0 0 2 0,41 ±0,01 0,24 ±0,02 2,39 ±0,05 0,49 ±0,01
Ham lugng diester alealoid (%)
0,25 ±0,01 0,044 ±0,002 0,033 ±0,003 0,18±0,01 0,037 ±0,002
Ham lugng aeonitin (%) 0,0094 ±0,0008 0,0021 ± 0,0002 0,0013 ±0,0002
0 0
Nhan xet:
- Phu tir sdng ed hdm lugng alealoid toan phin tuang ddi dn djnh vd eao, tuy nhien, hdm lugng nhdm chit dpc diester alealoid, aeonitin eao, trong do hdm lugng aeonitin dao dpng Idn.
- Ham lugng alealoid todn phin, diester alealoid, aeonitin trong ede m i u h i e phu, bach phu giam nhiiu so vdi phu tir sdng.
- Cao khd phu tir (ehiit nude) eo hdm lugng alealoid toan phin vd diester alealoid eao nhung kh6ng eon aeonitin,
Nghien cu'u tac dung cua cao hdc phu, cao bach phu, cao kho phu tip tren tim va mach vanh tho co lap
Bang 2: Tic dung cua cao hac phu, cao bach
Tim thd dugc co lap theo phuang phdp Langendoff vd truyin djch nuoi. Chd d i n khi tim trd lai boat dpng binh thudng, t i i n hdnh truyin dung djeh thude nghien cuu pha trong djch nuoi tim vdi ndng dp thich hgp b l n g may tiem truyin vdo dpng mach vdnh vdi tdc dp hdng djnh.
Ndng dp thudc trong djeh truyin tinh theo dugc lieu l i n lugt Id: Cao h i e phu 0,5%, eao bach phu 1%, cao khd phu tir 1 % .
Kit qua theo doi t i n sd dap eiia tim trong 1 phut bien dp co bdp eiia tim lien tue trong 30 phiit vd luu lugng djeh ehay ra qua tim trude, sau khi truyin thude vdo dpng mach vdnh tung 5 phiit mpt vd lien tuc trong 30 phut duge trinh bdy d bang 2.
phu va cao khd phu tu tren tim thd cd lap (n=8) Chi tieu theo ddi
TSn s6 tim
Bien dp tim (mm)
Luu luo'ng mach vanh (ml/5ph)
Mau nghien euu Cao h§c phu (0,5%) Cao bach phu (1%) Cao kho phu tir (1%)
Cao hac phu (0,5%) Cao bach phu (1%) Cao kho phu tir (1%)
Cao hac phu (0,5%) Cao bach phu (1%) Caokh6phutu(1%)
Trudv truyen thuoe
64,3 ±2,4 59,3 ±2,6 72,5 ±4,4 17,9 + 1,3 16,3±0,8 14,8 ±0,7 18,3±0,9 21,1 ±1,7 23,9 ±1,2
Sau truyen thuoe
59,6 ±2,5 57,5 ±3,4 67,3 ±5,1 23,4 ±1,2 21,4±1,5 20,6 ±1,2 20,1 ±0,9 23,6 ±1,5 27,4 ±0,9
Mue dd thay doi'(%)
4.7,3 i3,0 i7,2 T30,7 t31,3 t39,2 t9,8 t l 1 , 8 Tl4,6
P,-s
>0,1
• >0,5
>0,2
< 0,0005
< 0,001
< 0,0002
< 0,0005
< 0,0002
< 0,001 Ghi chii: I: giam, T: tang. Pis: P (truae - sau)
Nhan xet:
- Tae dung tren t i n sd tim: Cdc m i u c h i b i i n vd bdo c h i eua phu tu dugc nghien cuu: cao hie phu, eao bach phu vd eao khd phu tu d i u khdng lam thay ddi t i n sd tim thd co lap c6 y nghTa so vdi trude khi truyin thudc (P > 0,05).
- Tdc dung tren bien dp eo bop tim: Cdc m i u nghien cuu cd tdc dung tang bien dp eo bdp tim tu 30,7 % d i n 39,2 % so vdi trude khi truyin thudc, su khde biet cd y nghTa thdng ke vdi p < 0,001.
- Tdc dung tren luu lugng mach vdnh: Cdc m i u nghien cuu eo tae dung tang luu lugng mach vdnh tir 9,8 d i n 14,6% so vdi trude khi truyin thude, su khae biet ed y nghTa thdng ke vdi p < 0,001,
B a n l u a n
v i phuang phdp e h i biin h i e phu, bach phu vd bdo e h i eao kh6 phu tir: Nguyen lieu phu tu thu hoaeh tu cay 6 d i u da duge trdng dn djnh g Sa Pa (Lao Cai), cung chinh la nguyen lieu
trudc ddy dd sir dung trong nghien euu ehe biin d quy mo thi nghiem '^' "*' va se la ngudn nguyen lieu phue vu san x u i t edng nghiep. K i t qua nghien euu cho t h i y t u ngudn nguyen lieu nay, c h i biin h i e phu, bach phu d quy md 150 kg nguyen lieu tuai/me, bdo e h i eao kho phu tu' d quy m6 50kg phu t u phiin/me la kha thi. Hdc phu, bach phu dugc c h i biin tu nguyen lieu tuai nen phai thue hien tai vung nguyen lieu (Cong ty TNHH Traphaeo SaPa), vi vdy viee hudng d i n quy trinh e h i b i i n , k i i m tra, gidm sdt thue hien quy trinh ddi vdi phu tir, vj thudc cd dpc tinh cao c i n dugc t i i n hanh e i n than, ky ludng, kiim tra c h i t lugng san pham d t i t ca ede me c h i biin trudc khi nhap kho. D i i u nay da duge ede edn bp ky thuat tudn thu, san p h i m thu duge ddng d i u , quy trinh dn djnh. Thong qua nghien cuu, quy trinh c h i b i i n h i e phu, bach phu a quy mo pilot dd dugc xdy dung vd dp dung, h i c phu va bach phu dugc s u dung trong ede thang thudc theo dan thudc eua ede
TAP CHI DUQC HQC - 8/2010 (SO 412 NAM 50) 35
bde sT, luang y, Cao kh6 phu tu dugc bdo chi tai Cdng ty CP Traphaeo vdi mue tieu tao dugc san phim ddng nhlt phuc vu san xuit thudc d quy mo cong nghiep, Quy trinh bdo chi eao kh6 phu tu da duge xay dung vd dp dung eho san phim CO chit lugng dn djnh, lam nguyen lieu san xult vien nang bdt vj qui phu vd cdc ehi phim khae co phu tu,
v i kit qua djnh tinh alealoid: Cae miu nghien euu phu tu sdng, hie phu, bach phu vd cao khd phu tu diu cho phan ung duang tinh vdi eae thude thir chung ciia alealoid, hodn todn phii hgp vdi cdc kit qua nghien euu trude day d quy m6 thi nghiem '^'
v i ham lugng alealoid: Hdc phu, bach phu va eao kh6 phu tir duge ehi biin vd bao ehi d quy m6 pilot CO ham lugng alealoid todn phin, diester alealoid va aeonitin (tinh theo dugc lieu) giam nhiiu so vdi phu tu sdng, trong dd cao kho phu tu khong cdn aeonitin ehiing td eae alealoid eo dpc tinh cao da bj thiiy phdn nhiiu. Kit qua nay cung phu hgp vdi cac kit qua nghien cuu trudc day chi biin phu tir d quy md thi nghiem '^'^'.
v i tdc dung tren tim thd c6 lap: Hic phu, bach phu va cao kh6 phu tir (ndng dp thudc trong djch truyin tinh theo duge lieu lln lugt Id 0,5%; 1%; 1%) duge ehi biin va bao ehi d quy mo pilot diu khdng ldm thay ddi tin sd tim eo y nghTa thdng ke nhung diu eo tae dung gdy tdng bien dp tim va tang luu lugng mach vanh, Nhu vdy, viec ehi biin hie phu, bach phu vd bdo ehi eao kh6 phu tir d quy m6 pilot eho ede san phim CO tae dung cudng tim tren tim thd e6 lap, dam bao tieu chi bd "hda", bd duang eua phu tir ehi, phii hgp vdi kit qua nghien cuu trudc day ehi biin phu tir d quy md thi nghiem '^''''.
Alealoid todn phin la nhdm hoat ehit chinh eiia cdc ehi phim phu tir, tuy nhien, nhom diester alealoid va aeonitin la nhung chit co dpc tinh eao, cin loai bd trong qud trinh chi biin, bdo ehi, Theo kit qua djnh lugng co the tinh dugc hdm lugng alealoid toan phin trong djeh truyin cao hic phu, eao bach phu vd eao khd phu tir lin lugt la: 0,00205%, 0,0024% va 0,0049%, Kit qua nghien euu tae dung tren tim thd e6 lap cho thiy ty le gay tang bien dp tim cua eao hie phu, eao bach phu va cao kho phu tu lln lugt Id 30,7%; 31,3%
va 39,2% va ty le tang luu lugng mach vdnh lln lugt Id 9,8%; 11,8% vd 14,6%, Nhu vay, phai ehdng eo mdi quan he: ndng dp nhom boat chit ehinh alealoid toan phin trong djeh truyin cao se
lam tdng tdc dung tang bien dp tim vd tdng luu lugng mach vdnh.
K i t luan
Hdc phu vd bach phu duge ehi biin a quy md 150 kg nguyen lieu tuai/me, eao kho phu tu duge bdo che d quy mo 50kg phu tu' phiin/me tu ngudn nguyen lieu phu tu' Sa Pa. Kit qua djnh tinh alealoid, djnh lugng alealoid todn phin, diester alealoid va aeonitin trong ede san phim phu hgp vdi cdc kit qua nghien ciru trudc day d quy m6 thi nghiem. Cao hie phu, eao bach phu, eao kh6 phu tu co tdc dung gay tang bien dp tim, tdng luu lugng mach vdnh nhung khdng anh hudng din tin sd tim tren tim thd co lap. c i n xdy dung vung nguyen lieu phu tir Sa Pa dn djnh vd triin khai ehe biin phu tir, bdo ehi cao phu tir d quy md e6ng nghiep lam nguyen lieu eho san xuit thude.
Lai cam o-n: C6ng trinh ndy thupe Qk tdi hd trg kinh phi Nhd nude theo Nghj dinh 119/1999/NO-CP eua Chinh phii. Chiing toi xin ehdn thanh cam an Cdng ty CP Traphaeo va Bp Khoa hpe vd C6ng nghe dd tdi trg kinh phi, giiip chung tdi hoan thdnh c6ng trinh ndy.
Summary
This is a study on pilot processing Radix Aconiti carmichaelii lateralis from the cultivated plant of Sa Pa (Lao Cai) to get the heriDal items of Heishunpian and Baifupian in the traditional ways and producing Extraetum Aconiti therefrom. Analysis of their chemical composition showed a reamari<able reduction in content of all the total alcaloids, diester alealoids and aconitine; aconitine was not traceble in the obtained Extraetum Aconiti. The Heishunpian, Baifupian and Extraetum Aconiti all showed cardiotonic activities augmenting the myocardium contractility, cardiac output and no significant changes in heart rate on the isolated rabbit heart.
Key words: Phu tu, 6 diu. Hie phu. Bach
phu, Cao khd Phu tu, Heishunpian, Baifupian, Extraetum Aconiti, Aconitum carmichaelii Debx.
var. carmichaelii.
Tai lieu tham khao
1, Phiing Hoa Binh, Nguyin Trong Thong, Bui Hdng Cuong, Nghien cuu phuong phap ch§ biln va mot so tac dung sinh hoc cua vi thuoe Phu tir Sa Pa, Tap chi Dugc hoc (2003), (2), tr. 21-24.
2. Bill H6ng Cuong, Phung Hoa Binh, Vu Chi Nguyin & CS, Nghien ciru thanh phin hoa hoc, ham
lugng alealoid toan phan, diester alealoid cua Phu tu o Sa Pa, Tap chi Duac lieu (2005), 10 (2), tr. 55-59.
3. Biii Hong Cuong, Phiing Hoa Binh, Chu The Ninh, Djnh lugng aeonitin trong Phy tir Sa Pa bang phuong phap sac ky long hi#u nang cao (HPLC), Tgp chi Dugc hge (2006), (4), tr. 15-18.
4. Bill Hong Cuang, Phiing-Hoa Binh, Nguyen Trpng Thong, Nghien ciru tae dyng tren tim, mach
vanh va mach tai tho eo lap eiia mot so san pham cne bien tir Phu tir Sa Pa, Tap chi Dugc hoc (2007), (8), tr, 14-17.
5. The State Pharmacopoeia Commission of the People's Republic of China, Phamnacopoeia of the People's Republic of China (English Edition), Vol. I, People's Medical publishing house, Beijing, China, (2005) pp. 185-188.
Tac dung giam trong luo'ng va lipid mau
r r
tir mot so phan doan djch chiet qua so'n tra len chuot beo phi thuc nghiem
Nguyen Thj Thiiy Quynh', Do Ngoc Lien\
Nguyen Quang Huy''^ Nguyin Khac Tao^
' Khoa Sinh hoc, Trudng Dgi hoc Khoa hoc Tu nhien, DHQGHN Phong Enzym hoc vd Phdn tich hogt tinh sinh hpc, Phdng Thi nghiem trong diem Cong nghe Enzym vd Protein
^Trudng dgi hoc Su phgm Hd Noi 2
Ma dau
Theo Td chue Y t i t h i gioi (WHO), beo phi la tinh trang tieh luy md qud muc vd khong binh thudng tai mpt viing ca t h i hay todn than d i n mue anh hudng tdi sue khde. Cung vdi thua can beo phi, ede benh man tinh nhu ddi thdo dudng, tdng huyit dp, benh tim mach vd ung thu eung gia tang nhanh ehdng trong nhung ndm g i n day '^'. Benh thua can-beo phi dang tang len ro ret vd nhanh ehong khong nhung d cdc nude phdt triin md eon d nhung nude dang phdt triin. Theo k i t qua d i i u tra g i n ddy eua Vien Dinh duang cho t h i y tinh trang thua cdn beo phi d ngudi Viet Nam dang tang nhanh trong dd ty le beo phi d ngudi trudng thdnh nhit Id lua tudi tren 45 chiim ty le tdi 16,8% '^' Hien nay viee diiu trj benh bdo phi b l n g cdc loai thude tan duge duge dung hien nay tren th trudng thudng gdy nhiiu phan ung phu, va gid thanh r i t dit. Do dd, trong nhung ndm g i n day CO nhiiu cdng trinh nghien ciru su' dung cdc hgp chit tu nhien tu cay cd d i ehua benh beo phi vd dai thdo duong, bdi vi ede loai duge lieu tu cdy cd thudng it gay ra tdc dung phu, vd cd hieu qua trong thdi gian dai '^•^•''"•''^l.
San tra {Docynia doumeri Schneid) Id cdy thdn go thupe hp hoa hdng (Rosaceae), ed xuit xu tu viing 6n ddi Bdc bdn c i u tai chau Au, ehdu A vd Ble My, 0 Viet Nam, son tra phdn bd nhieu d cdc tinh miin niii nhu San La, Lao Cai, Cao Bdng, Yen Bdi...'^l T u xa xua, nhan ddn ta da biit su' dung san tra nhu mpt ngudn dugc lieu de ehua benh dudi dang thudc sdc, thudc bpt, thude cao hode rugu thudc. San tra thudng diing rieng mpt vj hoac phdi hgp vgi cdc vi thudc khac co tdc dung chua ede benh v i tieu hod u tre, d i y bung khd tieu, eao huyit dp vd giam md mau... '^'. Tuy nhien cho d i n nay, d Viet Nam ehua eo nhiiu cong trinh nghien euu sdu v i tac dung eua son tra noi ehung eung nhu v i tac dung Idm giam hdm lugng ehit beo trong mdu noi rieng. Trong bdo cdo nay chung tdi trinh bdy cdc nghien cuu bude d i u v i tdc dung cua mdt sd phdn doan djch chiit blng dung moi khac nhau tu qua son tra len ddi tugng chupt beo phi thuc nghiem Idm ea sd cho viec nghien cuu tach chiit cdc hgp chit cd boat tinh cd tac dung Idm giam qua trinh tich luy mo'.
Nguyen lieu va phu'ang phap
Qua son tra dugc thu hdi vdo thang 9 tai Td Xua (tinh Son La). Qua tuai dugc s l y kho va
TAP CHI DUQC HQC - 8/2010 (SO 412 NAM 50) 37
