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Fig. 2. Physical map of the restriction sites of the Coix 1.4 kbBglII/BglII fragment. Genes are represented by dark boxes.The four probes used in Northern and Southern blotting areindicated and correspond to nad3 (probe 1), rps12 (probe 2),tRNASer (probe 3
Fig. 3. Nucleotide sequence of the tRNASer/pseudo-tRNA/nad3/rps12 locus in Coix mtDNA (GenBank accession numberAF239263)
Fig. 4. RNA hybridizations of the nad3, rps12 and tRNASertranscripts in Coix. Five micrograms of total mtRNA weredenatured and applied to each lane of a 1.2% agarose/formal-dehyde gel, transferred to a nylon membrane and hybridized32P-labeled fragments of nad3 (1), rps12 (2) and tRNASer (3).An RNA ladder (9.5–0.24 kb, BRL) was used to estimatemolecular size.
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