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Fig. 1. Construction of the dicistronic FTR expression vector. The DNA fragments resulting from the amplification of cDNASUA with the primers SUAmin and SUAmeg and from the amplification of cDNA SUB with the primers SUBmin and SUBmegwere directly ligated in
Fig. 2. Western blot analysis of extracts of E. coli cellstransformed with pET-3d vector (1) or with vector carryingthe FTR construct (2)
Fig. 4, or photochemically using thylakoids and
Fig. 4. FTR dependent activation of fructose-1,6-bisphos-phatase. In a total volume of 100 �l 0.5 U of enzyme wereincubated in presence of 100 mM Tris–Cl pH 7.9, 1 �Mthioredoxin f, 5 �M ferredoxin, 14 mM 2-MET and varyingconcentrations of FTR, under argon

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