dan Industri Vol. XV, Th. 2004
BEKATUL
AWET
DAN
SECARA
IN
[In
Antioxidant Activity of Stabilized Rice Bran and Its Fraction]
Evy Damayanthi
Deddy Muchtadi
Fransiska Rungkat Zakaria
Syarief
C.
Hanny Wijaya
dan Djoko Said Damardjati
Staf Pengajar Departemen Gizi Masyarakat dan Sumberdaya Keluarga-FAPERTA-IPB, Kampus IPB Darmaga Staf Pengajar Departemen Teknologi dan Gizi- Kampus IPB Darmaga 16002
Ahli Peneliti Utama Bidang pada Pertanian, Jakarta
28 Agustus 2003 Disetujui 15 2004
ABSTRACT
Some researches indicated that oryzanol had however, the about the oryzanol role of low (LDL) and human lymphocyte from under stress was still The of this study was to the of oryzanol at concentratlons based on bran beverage model preventing LDL and lymphocyte from
Human plasma were the samples of bran unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 and oryzanol standard at the concentratlons of 308 3, 22 2, 5 2, 10 4 and 10 4 the human LDL were collected
by and a of 200 was reached Human LDL were then 5
for of the samples
The length of concentration, penod of sample supplemented human lymphocyte culture were before the of RBO and its in lymphocyte was measured The samples used in the lymphocyte were IR-64,
matter 1/3-64, and oryzanol standard at the concentratlons of 133 2 2,132 9 6 - 153 6 and 2 4
-
37The result showed that malonaldehyde concentration human decreased significantly 15 - and 39
-
56 compared to the control The absorbance of lymphocyte cell culture was not influenced by the type and of and itsThe of hydrogen 3 into culture lowered the absorbance as compared to culture
Key words . Oryzanol, oxidative stress, lymphocyte and antioxidant
PENDAHULUAN
Senyawa antioksidan alami dari berbagai
telah dibuktikan khasiatnya guna mencegah berbagai kerusakan oksidatif dan yang
kan reaksi radikal Radikal dan senyawa oksigen reaktif (SOR) terlibat dalam patogenesis berbagai penyakit, termasuk atherosklerosis (Kehrer, 1993).
Senyawa antioksidan antara lain kafeat pada biji kopi (Moon dan Terao, gingerol, shogaol dan pada ekstrak jahe (Fuhrman 2000; Septiana, kurkumin pada kunyit dan bangle (Mashuda et al., 1998; Nagano al., serta oryzanol pada bekatul padi 1999; dan Zhang, 1999).
Bahan-bahan yang berpotensi menyehatkan itu baik jika digunakan menjadi produk makanan dan
atau disebut dengan
fungsional. Bentuk fungsional yang populer adalah
Penelitian mempelajari aktivitas antioksidan y- oryzanol yang terkandung dalam bekatul. Xu et al., (2001) menunjukkan aktivitas antioksidan y-oryzanol kuat dibandingkan vitamin E- yang juga pada minyak bekatul-pada oksidasi kolesterol secara in Penelitian y-oryzanol pada binatang percobaan dan manusia membuktikan bersifat hipokolesterolemik (Kahlon dan Chow, 1997). tetapi informasi aktivitas antioksidan y-oryzanol untuk melindungi oksidasi lipoprotein dan sifat perlindungan terhadap sel dari penderitaan akibat stres oksidatif belum ada.
