THE GROWTH OF ORCHID (DENDROBIUM SP) IN VITRO GIVING WITH COCONUT WATER ON DIFFERENT MEDIUM.

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Fakultas Matematika dan Ilmu Pengetahuan Alam

The Center of Excellency

PROCEEDING

First Annual International Seminar on

Trends in Science and Science Education 2014

5

th

–

6

th

December 2014

Garuda Flaza Hotel - Medan

Editors:

Prof. Dr. Herbert Sipahutar, M.Sc.

Prof. Drs. Motlan, M.Sc, Ph.D.

Prof. Drs. Manihar Situmorang, M.Sc., Ph.D.

Prof. Dr. Muktar, M.Pd. Alkhafi

Maas Siregar, S.Si., M.Si. Drs.

Zulkifli Simatupang, M.Pd

Universitas Negeri Medan

2015

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The Center of Excellency

Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

The Character Building University FROM THE EDITORS

The First International Seminar on Sciences and Science Education, ISOSE, organized

by Faculty of Mathematics and Natural Science of State University of Medan, was held on 4 – 5

December 2014 in Medan, North Sumatera, Indonesia. The seminar particularly encouraged the interaction of research students and developing academics with the more established academic community in an informal setting to present and to discuss new and current work. The high quality of the papers and the discussion represent the thinking and experience of experts and practitioners, researchres, lecturers and students in their particular fields and interests. The papers contributed the most recent scientific knowledge known in science and science education.

This proceeding contains all the paper presentated in the seminar, consisted of 11 papers of Biological Sciences, 11 papers of Chemical Sciences, 3 papers of Mathematical Sciences, 14 papers of Physical Sciences and 39 papers of Science Education.

In addition to the contributed papers, an outstanding keynote presentation on National Curriculum 2013 was made by Prof. Dr. Syawal Gultom (formerly Rector of State University of Medan, Unimed), now as Head of Badan Pengembangan Sumberdaya Manusia Pendidikan of Department of Education and Culture of Republic of Indonesia. This presentation gives all pratisipants a new and comprehensive perspective on the orientation of national education in the next era.

Two invited keynote presentations were given by Prof. Dr. Yaya Rukayadi from Department of Food Science, Faculty of Food Science and Technology and Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor DarulEhsan, Malaysia who spoke on how to appreciate the nation through research javanese

turmeric or temulawak (Curcuma xanthorrhiza ROXB.), and by Dr. Phattrawan Tongkumchum,

Department of Mathematics and Computer Science, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand who spoke about the applications of the weighted sum contrasts methods on graphing confidence interval for adjusted mean, their used for comparing two and several groups, and adjustment for covariates.

We would like to express our deep appreciation to Prof. Dr. Ibnu Hajar, Rector of State University of Medan for financial support by means of Dana DIPA Unimed FY 2014.We would like to express our deep appreciation to Prof. Dr. Motlan (Dean of FMIPA Unimed), all sponsors, all member of seminar committe, that make the seminar happen in a great succes.

We thank all authors and participants for their contributions.

Medan, February 2015

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The Center of Excellency

The _{Character Building}_University 2

FROM THE CHAIRMAN OF THE ISOSE 2014

Your Excellency, Bapak Prof. Ibnu Hajar Damanik, Rector of Universitas Negeri Medan (State

University of Medan)

Your Excellency, Bapak Prof. Khairil Ansari, Academic Affair Vice Rector of Universitas Negeri

Medan

Your Excellency, Bapak Chairul Azmi, Financial Affair Vice Rector of Universitas Negeri Medan

Your Excellency, Bapak Prof. Biner Ambarita, Students Affair Vice Rector of Universitas Negeri

Medan

Your Excellency, Bapak Prof. Berlin Sibarani, Cooperation Affair Vice Rector of Universitas

Negeri Medan

Your Excellency, Bapak Prof. Motlan, Dean of Fakultas Matematika dan Ilmu Pengetahuan

Alam, Universitas Negeri Medan

Your Excellency, Bapak and Ibu Deans of Faculties in Universitas Negeri Medan

Your Excellency, Bapak Prof. Drs. Manihar Situmorang, M.Sc., Ph.D., Head of Research

Institute of Universitas Negeri Medan

Your Excellency, Bapak Dr. Ridwan Abdullah Sani, M.Si., Head of LPM of Universitas Negeri

Medan.

Distinguished experts, researchers, lecturers, students, guests, and all participants, ladies and

gentlemen.

Good morning and welcome to Medan, North Sumatera.

Before I begin with a few words please understand that our keynote speaker, His excellency

Bapak Prof. Syawal Gultom, Head of Badan Pengembangan Sumberdaya Manusia Pendidikan

is on the way right now. Hopefully, this does not lessen the meaning of this seminar, because

he will be with us in the next several minutes and gives his talk in this room.

First, let us be thankful to the one and all-powerful God that on this fine morning we are still

given bodily and spiritual health and can gather together in this room, on our beloved capital city

of North Sumatra, Medan.

Next, to our invited speakers Prof. Yaya Rukayadi (from University Putra Malaysia), Prof.

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The Center of Excellency

The Character Building University (from Prague University, Check) we also want to extend a warm and special welcome to this

seminar.

This seminar, International Seminar on Trends in Science and Science Education 2014 is

specially organized by Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri

Medan. The seminar is planned to be held regularly on annual based as an attempt of FMIPA

Unimed to prepare a good vehicle to our lecturer, researchers, students and all human

resources to communicate, exchange and disseminate their experiences at the international

level. The main objective of this seminar is to encourage collaboration among the different

actors in science and science education community so as to achieve a better result for the

benefit of the community. This first annual international seminar will be held from December 5 –

6, 2014.

It is expected that those who participate in the seminar will afterwards be familiar to be able to

interact with their international counterparts in their scientific area. To make this interaction

happen, the lecturer, researchers, and students should be able to improve the research quality

in their respective domains. Hopefully, by organizing the regular international seminar, will help

not only Fakultas Matematika dan Ilmu Pengetahuan Alam but also Universitas Negeri Medan

to realise its dream to a world class and character building university.

Approximately 300 participants from four different countries are attending this seminar. They

come from Chech Republic, Thailand, Malaysia, and of course from Indonesia. Most of the

participants are lecturers, researchers, teachers and students, so we hope that a deep mutual

interaction will unfold. The participants come from different backgrounds and institutions. Some

are from government institutions, some from research institutions and universities, and some

are from NGOs.

Approximately 100 seminar abstracts and full papers from science education, biology,

chemistry, physics, and mathematics sciences have been received by the seminar committe.

Most of the abstract have been edited and bound into an abstract collection book which is a part

of the seminar kit.The seminar full papers are now in editing stage by the committe before

publish in seminar proceeding that will be available in both printed and on-line forms, in the next

January 2015. Please, remind the committe if you want to get the copy of the seminar

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The Center of Excellency

I would like to take this opportunity to acknowledge the important role of the honorable Prof. Ibu

Hajar, rector of Universitas Negeri Medan for giving us his full support and attention and for

providing his precious time to be with us and to honour us by opening this seminar.

Our sincere thanks also goes to the honorable Prof. Motlan, Dean of Fakultas Matematika dan

Ilmu Pengetahuan Alam, who have lead and encourage all the committe members to be always

focussed and worked hard even in a very short period of time to prepare the seminar.

I would also like to thank His Excellency Prof Syawal Gultom, former Rector of Universitas

Negeri Medan, and now Kepala Badan Pengembangan Sumber Daya Manusia Pendidikan,

Departemen Pendikan dan Kebudayaan Republik Indonesia, for being willing to share his

experiences, knowledge and skills with us.

My sincere thanks also goes to all members of the committe and to all staff of Fakultas

Matematika dan Ilmu Pengetahuan Alam for their continuous support and hard work because

without their assistance this seminar may not have taken place today.

As Newton stated in his second law of motion, “Every body continues in its state of rest, or of

uniform motion in a straight line, unless it is compelled to change that state by forces impressed

upon it”. It is time for us (as lecturers, researchers, students and all staffs of the faculty or

university ) to apply the necessary force to our specific daily role in order to make our motions

converge more closely. Where better to start than on science and education, which will form the

foundation of our development and character as the 21st century unfolds.

Finally, I conclude my speech by kindly inviting Honourable Prof. Ibnu Hajar Damanik, Rector of

Universitas Negeri Medan, to give special direction and officially open the seminar.

We wish you good luck and success in this endeavour.

Thank you for your attention.

Medan, December 5, 2014

Herbert Sipahutar

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The Center of Excellency

The Character Building University

TABLE OF CONTENT

FROM THE EDITORS ii

FROM THE CHAIRMAN OF ISOSE 2014 iii

TABLE OF CONTENT vi

Keynote Speaker

KS-001 Dasar Pertimbangan Penetapan Struktur Kurikulum 2013

Prof. Dr. Syawal Gultom, M.Pd. 1 - 10

Invited Speaker

IS-001 Confidence Intervals with Application to Environmental Studies in Southern Thailand

Phattrawan Tongkumchum

11 - 21 IS-002 Appreciate the Nation Through Research Javanese Turmeric or Temulawak

(Curcuma xanthorrhiza ROXB.): Xanthorrhizol an “Angel” Compound in the Rhizome of Temulawak and Its Applications

Yaya Rukayadi 22

Biological Science

BS-001 Isolation of Heat Shock Proteins Gene (HSPs-gene) in the Silkworm, Bombyx mori (C301)

Masitta Tanjung, Maryani Cyccu Tobing, Syafruddin Ilyas, and Darma Bakti 23 - 28 BS-002 Mating Behavior of Male Mice After MSG Administration during Intra Uterine to

Mature Periods of Life

Herbert Sipahutar and Adriana Y.D. Lbn Gaol 29 - 38 BS-003 Ecobiological Review of Neolissochilus sumatranus (Ikan Batak) (Weber and de

Beaufort, 1916) In Asahan River, North Sumatera

Ternala Alexander Barus, Hesti Wahyuningsih, Eva Marlina Ginting, and Charles

PH Simanjuntak 39 - 45

BS-004 The Growth of Orchid (Dendrobium sp) in In Vitro Giving with Coconut Water on Different Medium

Fauziyah Harahap 46 - 53

BS-005 Diversity of Lichens on the Stands of Mahoni (Swieteniamacrophylla) Functioning as Shade Plants in Medan

Ashar Hasairin, Nursahara Pasaribu, Lisdar I. Sudirman, and Retno Widhiastuti 54 - 60 BS-006 Studies on Species Diversity and Growth Rate of Mold in Musk Lime Pickle

(Citrofortunella microcarpa) Food Society of Melayu

Mhd. Yusuf Nasution and Ashar Hasairin 61 - 67 BS-007 In Vitro Selection Uplandrice Nias Island to Aluminium Resistant Character and

Low pH through Somaclonal Variation and Gamma-Rays Irradiation

Syahmi Edi, Lazuardi and Idramsa 68 - 77

BS-008 Cassava Leaves Battery as Alternative Energy Based on Environment Friendly Technology

M. Gade 78 - 81

BS-009 Description of Endophytic Fungi of Plants Raru (Cotylelobium melanoxylon ) Genus Alternaria

Uswatun Hasanah, Riwayati and Idramsa 82 - 90

BS-010 The Effect of Biji Mete (Scomberomorus commerson) in the Feed to the Decline in Blood Cholesterol Level Hypercholesterolemic Male Mice (Mus musculus)

Rudi Kartika and Eddiyanto 91

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The Center of Excellency

BS-011 Frequency Attendance of Makrozoobentos with Physics Factor and Chemistry in Territorial Water of Babura River

Masdiana Sinambela and Mariaty Sipayung 92

BS-012 Inventory of Nematophagous Fungi in Sumatera Utara, Indonesia

Liana Dwi Sri Hastuti, Jane Nicklin and Ameilia Zuiyanti Siregar ₉₃

BS-013 Land Degradation in the Upstream of Deli Watershed in North Sumatera Based on Soil Erosion Rate Prediction and Soil Erosion Hazard Level

Sumihar Hutapea; Ellen Lumisar Panggabean and Endang Sari Simanullang 94 BS-014 Diversity and Abundance of Insect Pollinators in Different Agricultural Lands in

Jambi, Sumatera

Elida Hafni Siregar, Tri Atmowidi, and Sih Kahono 95 BS-015 Biodiversity of Leaf-and Planthopper (Hemiptera: Auchenorrhyncha) on Rice

Ecosystem at High Land Tapanuli Of North Sumatera-Indonesia

Binari Manurung, Puji Prastowo, and Erika Rosdiana 96

Chemical Science

CS-001 The Binding Behaviour and Conformation of Rhodobacter sphaeroides TSPO in DDM and DPC Detergents

Nora Susanti, Joshua Sharpe and Krisztina Varga 97 - 102 CS-002 Synthesis, Crystal Structure and Magnetic Properties of the Spin Transition

System [Fe(pq)3](ClO4)2 Complex

Iis Siti Jahro, Djulia Onggo, Bohari M. Yamin, Ibrahim Baba and Nandang Mufti 103 - 109 CS-003 The Utilization of Tamarillo Peels as Matrix of Bacterial Cellulose-Based

Nanopaper

Joshua and Saharman Gea 110 - 114

CS-004 Isolation and Characterization of α-Cellulose of Rice Leaves

Yusnaidar, Basuki Wirjosentono, Thamrin, and Eddiyanto _{115 -119}

CS-005 Inhibitory Activity of Alkaloid of Extract Ethanol Ranti Hitam (Solanum blumei

Nees ex Blume) Fruit ON Leukimia L1210 Cancer Cells Growth

Murniaty Simorangkir, Ribu Surbakti, Tonel Barus and Partomuan Simanjuntak 129 - 126 CS-006 The Isolation of Nanocrystalline Cellulose from Palm Empty Fruit Bunches

Mahyuni Harahap, Fenny Aulia, and Saharman Gea _{127 - 131}

CS-007 Study of Rubber Seed Oils Hydrocracking into Biogasoline and Diesel Fraction Over the Combination Y-Zeolite and Ni Catalyst

Ary Anggara Wibowo, Salsabila Firdausyah, Siti Hajjah, Dina Dwiyanti, Junifa

Layla Sihombing, Ahmad Nasir Pulungan 132 - 140 CS-008 Compregnated Oil Palm Trunk (Elaeis guineensis Jacq.) with 20% Dammar

Resin (Agathis dammara)

Nurfajriani, Leni Widiarti, and Basuki Wirjosentono 141 - 144 CS-009 The Activity Values of Cla (Conjugated Linoleic Acid) Synthesized from Castor

Oil by Using Visible Spectrofotometer with DPPH as Free Radical

Marham Sitorus and Bajoka Nainggolan 145 - 147 CS-010 Preparation, Characterization and Activity Assay of NiO-CoO-MoO/Zeolite-Y

Catalyst on Hydrocracking of Cashew Nut Shell Liquid in Fixed-Bed Reactor

Ahmad Nasir Pulungan, Junifa Layla Sihombing, Hafni Indriati Nasution, Ratu

Evina Dibyantini, Rini Selly, Wega Trisunaryanti, and Triyono 148 - 154 CS-011 Effect of Temperature and Composition Zeolite on the Performance Membrane-

Zeolite for Separation of Ethanol-Water by Pervaporation

Ridwanto, Rahmat Nauli, Ani Sutiani, and Anny Sartika Daulay 155 - 163 CS-012 Biofuel Production from Hydrocracking MEFA of Rice Bran Oils Over Natural

Zeolit Supported Ni and Ni-Mo Metals

Junifa Layla Sihombing, Jasmidi, Nurmalis, Ahmad Nasir Pulungan, and Ratna Sari

Dewi 164

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Universitas Negeri Medan The _{Character Building}_University 8

Oxypropylation Reaction

Abubakar, Basuki Wirjosentono, Thamrin, and Saharman Gea

CS-014 Characterization and Utilization of Bentonite Sabang of Aceh (Indonesia) as Fillers Polymer Nanocomposite

Saharman Gea, Julinawati, and Basuki Wirjosentono 166 CS-015 Efficiency and Selectivity Improvement of the Silica Chitosan Hybrid by pH

Optimilization in Simultan Heavy Metals Mg(II) and Ni(II) Adsorbtion Using Solid Phase Extraction Method

Lisnawaty Simatupang 167

CS-016 Natural Rubber Modification: Graft-Copolymerization of Cyclic Natural Rubber by Free Radical Reaction with Maleic Anhydride

Eddiyanto, Alkhafi Maas Siregar, and Winsyahputra Ritonga 168

Mathematical Science

MS-001 An Active Constrained Based Approach for Solving Problems for Positioning New Products Under Risk

Nerli Khairani 169 - 178

MS-002 An Integrated Optimization Model for River Water Quality to Estimate Wastewater Removal

Syafari 179 - 188

MS-003 An Improved Approach for Solving the Plant Cycle Location Problem

Agusman _{189 - 192}

Physical Science

PS-001 A Comparison of Methods for Testing Homogeneity of Average Temperature and Precipitation Series

Marzuki Sinambela and Esty Suryaningsih 193 - 200 PS-002 Mapping for Groundwater Potential Based on Resistivity Data Interpretation in

Pamah Paku Kutambaru Langkat Regency

Hengki Sembiring and Rita Juliani 201 - 209

PS-003 Lithology of Ketuken Watershed in Langkat

Rochayanti N R Simatupang and Rita Juliani _{210 - 217}

PS-004 Anthrophegenic Causes Analysis on Heavy Metal Pollution in River Water and Sea Water in Middle Tapanuli Regency North Sumatera

Rahmatsyah, Eddy Marlianto, Mester Sitepu, and Motlan 218 - 227 PS-005 Morphological Analysis and Content Elements of Limestone from Village Sulkam

Langkat Using Scanning Electron Microscope (SEM)

Rita Juliani, Timbangen Sembiring, Mester Sitepu, and Motlan 228 - 238 PS-006 Influence of Concentration and Post-Heating to the Crystal Size and Optical

Properties of ZnO Thin Films

Nurdin Siregar, Eddy Marlianto, Saharman Gea, and Nurul Taufiqu 239 - 248 PS-007 Synthesis and Characterization Optical Properties of Cu2O Nanoparticles with

Coprecipitation Method Based Concentration Variations Precipitator

Pintor Simamora, Juan R. S., and Berton M. Siahaan 249 - 259 PS-008 Thermal Analysis and Structure of Nano Composite Palm Oil Boiler Ash

Eva Marlina Ginting and Nurdin Bukit _{260 - 269}

PS-009 Effect of Rice Husk Ash and Palm Oil Boiler Ash as a Mixture on Concrete Porosity

Karya Sinulingga and Satria Mihardi 270 - 278 PS-010 Geoelectric Investigation Schlumberger Configuration of Limestone Distribution

in Cangap Kerabangen Area Kutambaru Subdistrict Langkat Regency

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Geothermal Area Tinggi Raja Simalungun, North Sumatera Province Using 2D Resistivity Imaging and XRD

Muhammad Kadri and Eko Banjarnahor

PS-012 Correlation of Spectral Reflectance Characteristic Based on Spectrometer Cropscam MSR 16R and Satellite Image Landsat TM (Study Case In Medan- Indonesia)

Togi Tampubolon 297 - 305

PS-013 Sensitivity of Breaks for Additive Seasonal and Trends (BFAST) Method to Detect the Vegetation Changes Based on the Choice of Vegetation Indices and Land Cover Types

Yahya Darmawan, Esti Suryaningsih, and Lamtupa Nainggolan 306 - 314 PS-014 Influence of Sintering Time on the Properties of High Temperature

Superconductor BPSCCO Based

Eidi Sihombing 315 - 319

PS-015 Resistance Analysis of Rock and Mineral Under Surface by Using Geoelectric Method in the Village of Dolok Marawa Simalungun Distric

Abd Hakim S and Marausaha Simanjuntak 320

Science Education

SE-001 Impact of Phet Simulation Media to Minimize Quantity Misconceptions Students in Learning Dynamic Electrical Material

A.Halim, Azzarkasyi and Ibnu Khaldun 321 - 327 SE-002 Misconception on Biology Materials Among Biology Teachers and Science

Students of Senior High School in North Sumatra

Adriana Y.D. Lbn Gaol and Herbert Sipahutar 328 - 337 SE-003 Misconception Reduction Effectiveness in Physics Learning Through Laboratory

Working Methods in the Concept of Temperature and Heat

A.Halim, Qusthalani and Ibnu Khaldun 338 - 343 SE-004 The Effect of Problem Based Learning Model Toward Students’ Science Process

Skills in Senior High School

Turnip Betty, Simanjuntak Mariati Purnama, and Purba Frikson 344 - 350 SE-005 The Development of Media Device on Problem Based Learning Applied

Microbiology

Hasruddin and Mahmud 351 - 358

SE-006 The Effectiveness of Using Developed Problem Based Learning Tools on General Physics II of Physics Student, State University of Medan

Jurubahasa Sinuraya, Sehat Simatupang, and Ida Wahyuni 359 - 367 SE-007 Application of Resource Based Learning Model for Improving Learning

Outcomes Student in the Cube and Beams Matter in Class VIII SMP Negeri 5 Stabat T.A. 2013/2014

Asrin Lubis 368 - 378

SE-008 The Difference of Mathematical Problem Solving Achievement of Public Junior High School Based on Learning Approach

Ani Minarni 379 - 386

SE-009 The Effect of Cooperative Learning Model Type Group Investigation on Student’s Achievement of Static Fluid in Class XI of SMA Negeri 1 Perbaungan A.Y. 2013/2014

Derlina and Rikcy Almeda 387 - 393

SE-010 Effect of Cooperative Learning Model Type Numbered Heads Together (NHT) Assisted Animation Media of Student Learning Outcomes

Mariati Simanjuntak and Rebecca Sianturi 394 - 402 SE-011 Effect of Project Based Learning Model with KWL Worksheet on Divergent

Thinking in Solved Physics Problems

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Universitas Negeri Medan The _{Character Building}_University 10

Through Realistc Math Approach

Muhammad Arif Hidayat

SE-013 The Influence of Inquiry Training Learning Model Toward Students’ Achievement on the Topic of Heat in Class X Semester II MAN Kisaran A.Y. 2013/2014

Sehat Simatupang and Ika Nurjannah Sirait 420 - 428 SE-014 Analysis Implementation of Practical Approriate with Standard of Competency

Biological Lesson and the Application at Class X in SMA Negeri 11 Medan Tembung in Academic Year 2009/2010.

Venisha E.A. Pardede and Tri Harsono 429 - 436 SE-015 Analysis of Competency and Performance of Secondary Teachers: Case Study

at Five Cities/Districts in South Sumatra

Umi Chotimah, Zahra Alwi, and Farida 437 - 445 SE-016 An Intensive Study of Teaching Model of Quantum Physics at Study Program of

Physics Education in University

Mara Bangun Harahap 446 - 452

SE-017 The Effectivenees of Laboratory Experiment Method to Increase Activity and Student’s Achievement on Teaching Salt Hydrolysis

Ramlan Silaban, Agustina M.L. Tobing, and Irving Josafat Alexander 453 - 460 SE-018 Contribution of Formal Thinking Ability on the Concept Mastery of Kinematics

Sondang R Manurung _{461 - 469}

SE-019 The Use of Equation Worked Examples for Solving Electrochemistry Problem

Erdawati _{470 - 477}

SE-020 The Effect of Predict Observe Explain Strategy (POE) on Students Activity and Learning Outcome on Human Respiratory System Sub Topic in Grade XI Science Program at SMA Negeri 15 Medan Academic Year 2013/2014

Suyedi Hendra Yanto and Syahmi Edi 478 - 484

SE-021 The Difference Between the Ability of Students in Solving Problem by Applying Cooperative Learning Type STAD With and Without the Help of Geogebra

Sinta Dameria Simanjuntak 485 - 493

SE-022 Effect of Creative Learning Techniques and Reasoning Ability Toward Student Achievement in Physics

Purwanto 494 - 507

SE-023 Improving Speaking Ability of the Students in English Lessons by Using Learning Model TPS (Think Pair Share) in Class V SD Immanuel Medan

Naeklan Simbolon, Fridawati R. Tambunan 408 - 517 SE-024 The Application of Quantum Model Learning to Improve Student Learning

Motivation on Science Subject State Class V SD 064 978 Medan Denai

Nani Barorah Nasution 518 - 523

SE-025 Group Investigation Assisted E-Learning: Assessing the Impact of Interactive Media on Student’s Learning Achievement and Critical Thinking

Wenny Pintalitna and Herbert Sipahutar 524 - 532 SE-026 A Gametogenesis Module Development in POE (Prediction, Observation and

Explanation) Oriented Model

Hafizah Ilmi Sufa and Meida Nugrahalia 533 - 539 SE-027 Scientific Creativity in Learning Biology in Senior High School Tebing Tinggi City,

North Sumatra

Widya Arwita 540 - 546

SE-028 Application of Cognitive Theory of Content on Learning Ability to Increase Physical Science Using Generic Injection Needle Kit

Nurmaulita 547 - 556

SE-029 The Perceptions of Global Warming and Environmental Benefits of Biodiesel for Sustainable Energy Among High School Students in Jakarta

Desnita 557 - 565

SE-030 Ability Profile of Multiple Representations (MR) Students of Teacher Prospective

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1 Proceeding: The First International Seminar on Trends in Science and Science Education 2014 – ISBN 978-602-9115-37-6

Nurliana Marpaung and Liliasari

SE-031 Low Achievement of Indonesian Student in PISA and TIMSS Test Results and the Related Factors

Ridwan Abdullah Sani 572 - 588

SE-032 The Effect of Guided Discovery Based Learning Model Towards Students Learning Outcomes of Chemistry on Redox Reaction Concept

Anna Juniar, Dede Suriyani, Pravil Mistryanto, and Debby Masteriana 589 - 595 SE-033 A New Breakthrough in Chemistry and Management

Wesly Hutabarat 596 - 604

SE-034 Evaluation of Sports Training Program Guidance Center Student (PPLP) North Sumatra

Sabaruddin Yunis Bangun 605 - 615

SE-035 The Characteristics of Theology of Moslem Batak Toba in State University of Medan

Ramli Nur, Usman Pelly, Hasan Bakti Nasution 616 - 625 SE-036 Pengembangan Model Pembelajaran Hybridlearning Mata Kuliah Pengantar

Sosiologi di Universitas Negeri Medan

Trisni Andayani 626 - 630

SE-037 Pre Competency Test Standardization on Program of Field Experience HAT Competency-Based Graduate Education Through Item Analysis

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NIP. 1 026 199103 1001

(17)

Lampiran 8

LEMBAR

HASIL

PENILAIAN

SEJAWAT SEBIDANG

ATAU

PEER REVIEW

KARYA

ILMIAH

Judul Makalah

Penulis Makalah

Identitas Makalah

:

Fauziyah Harahap

:

a. Judul Prosiding

b.ISBN

c. Tahun Terbit

d. Penerbit

e. Jumlah halaman

:

'orhe Growth

of

orchid

(Dendrobium Sp) rn

In

vitro

Giving

with

coconut Water On Different Media"

Abstract Intemational Seminar

Decernber 2014

FMIPA Unimed

60 B-09

Kategori Publikasi

Makalah p]

(beri /pada _{kategori yang tepat)}

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Prosiding Forum Ilmiah Nasional

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7,9

Totat

=

(100%)

17

Medan,

September 2015

Reviewer - 3

Prof. Dr. Herbert Sfahutar, MS, M.Sc

NIP. 19610626 198710 1001

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Universitas Negeri Medan The _{Character Building}_University

46

BS-004

THE GROWTH OF ORCHID (

Dendrobium sp

) IN

IN VITRO

GIVING WITH

COCONUT WATER ON DIFFERENT MEDIUM

Fauziyah Harahap

Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Medan, Jln. Willem Iskandar Psr V Medan Estate, 20221, Indonesia

E-mail: fauziyahharahap@gmail.com

ABSTRACT

This study aimed to determine the effect of coconut water on the growth of in vitro orchid

shoots (Dendrobium Sp) on Murashige and Skoog medium, medium containing foliar fertilizer

(Grow More (GR) = N: P: K = 20: 20: 20). Research has been carried out in the Laboratory of "YAHDI" Plant Tissue Culture and Biology Departement of Unimed. This research design using non factorial completely randomized design with treatments namely: The first setting: MS Medium with 6 levels coconut water, namely 0%, 5%, 10%, 15%, 20%, 25%, the second : Medium containing Grow More (GR) 2 g/l with 6 levels coconut water, namely 0%, 5%, 10%, 15%, 20%, 25%. Each combination treatment was repeated 4 times. Parameter observations in this study was : the number of shoots, number of leaves, number of roots, shoots performance. The results showed that there are significant effect of medium, coconut water to all parameters:

the number of shoots, number of leaves and number of roots of the Dendrobium orchid. Shoots

performance was pale green shoots for giving of low doses of coconut water, will be green with coconut water with medium doses, the higher the concentration of coconut water will make the shoots appear stunted and eventually died.

Keywords: MS medium, coconut water, Dendrobium Sp, Grow More

INTRODUCTION

Orchids belonged flowering plants, widespread in parts of the world, including

Indonesia. Indonesia is a country that has the number and types of orchids are very diverse.

Donations and participation of Indonesian Orchid in the Orchids world is a big. It is estimated,

orchid species spread throughout in the world amounted to 20,000 species and 6.000 species

of which are in the Indonesian. Besides orchid species, we known also some orchids in the

cross or hybrid. It is estimated that annually, produced 1.000 new hybrid. Orchids have a high

economic value when compared to other ornamental plants (BPPP, 2004)

Dendrobium is one of the family Orchidaceae plants are much favored by consumers.

Popularity of this orchids is due to the variety of colors and the beautiful flowers, fresh flowers

also caused a period longer than other ornamental plants (Amalia, 2013). Dendrobium orchids

are plants from tropical regions that require sunlight and temperatures hot enough, not like a

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temperature 28-30oC (a minimum temperature of 15oC), with light is 50-60%. Dendrobium need

environment is not too wet, but require high humidity at 65% - 70% (Kartiman, 2004).

The genus Dendrobium has a high diversity on habitat, size, pseudobulb, leaf and

flower color. Spread spectrum is wide, from coastal areas to the mountains. Spread in India, Sri

Lanka, China, Japan, Southeast Asia, to the Pacific region, Australia, New Zealand, and Papua

New Guinea. It grows well at altitude 0-500 m above sea level with 60-80% humidity (Linga, P

and Marsono. 2001).

Simpodial type Dendrobium orchid

Figure 1. Ferformance of Dendrobium

Dendrobium with the long pseudo bulb dan flower

This orchid has a long pseudo bulb. Leaf intersect along the pseudo bulb. According to

the nature of the growth of this orchid including simpodial types, forming clumps and roots in

every pseudo bulb. The flowers often appear at the apical bud. However, in mature plants, the

flowers some time appear in the axilar.

Tissue culture method was developed to help reproduce plants, especially for plants

that are difficult to breed in generative. Plantlet produced from tissue culture has several

advantages, namely, identical to the parent, can be reproduced in large quantities, not to need

a large place, producing a large plantlet in a short time, plantlet quality is guaranteed, and the

seedlings grow faster compared with conventional propagation (Anonymous, 2010).

Tissue culture is a plant propagation technique that greatly influenced by the content of

the substante in medium. To maximize the growth process, used hormones or plant growth

regulators. Plant growth regulator (PGR) has a role in the process of growth and development

for the survival of a plant (Nugroho, 2000, Harahap, 2011).

Coconut water is the liquid endosperm or food reserves of energy sources in the

coconut. It is a complex organic substance that is always used in plant propagation by tissue

culture techniques. This complex substance is often used, but have not obtained the right dose

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48

Coconut water, indicated to contain high endogenous cytokines, can induce the plant

shoots. Recent research get content of coconut water, namely: amino acids, organic acids,

nucleic acids, purines, sugars, alcohols, vitamins, minerals, growth regulators. Coconut water

contain plant growth regulator (Gunawan, 1992): 9-B-D ribofuranosyl zeatin, Zeatin, N-N-

Diphenyl urea, 2 (3-methyl-but-2 etyl amino) 6-purin one. The content of growth regulators in

coconut water is beneficial to induce callus and induce morphogenesis process (Pisesha,

2008).

Growmore is a foliar fertilizer that is always used for spraying on orchid plant. This

fertilizer contains N : P : K with ratio 40: 20: 20. Generally to stimulate vegetative growth

(Fahruroh, 2008). In this study Growmore replaces of MS medium for plant tissue culture. In the

commercial treatment, using growmore can be saving the operational cost. So that the potential

for business development. This fertilizer is a potential can be developed through this research,

because growmore containt high Nitrogen that needed for orchid growth. Nitrogen is a nutrient

that is needed primarily for vegetative growth. Plants absorb N is generally in the form of

ammonium (NH4+) and nitrate (NO3). Excessive N application will be effect to vegetative growth,

leaf color becomes dark green and more succulent (Fahruroh, 2008).

The purpose of this study was to determine the growth of in vitro orchid shoots after

giving the Murashige Skoog (MS), Growmore (GR) medium and added coconut water.

METHODOLOGY

This research was conducted at the YAHDI Laboratory of Tissue Culture, Medan, and

Biology laboratory UNIMED Medan, North Sumatera. Indonesia. The materials used are in vitro

Dendrobium Sp plantlets, MS medium, Growmore, alcohol, sterile distilled water, detergent,

coconut water.

Standard tissue culture tools were used for this research. This research design using

non factorial completely randomized design with treatments namely: The first setting: MS

Medium with 6 levels coconut water, namely 0%, 5%, 10%, 15%, 20%, 25%, the second:

Medium containing Grow More (GR) 2 g/l with 6 levels coconut water, namely 0%, 5%, 10%,

15%, 20%, 25%. Each combination treatment was repeated 4 times.

Shoots were planted on the shoot induction medium according to the treatment. The

culture was maintained at 24°C by regulating the room air conditioner. For maintaining the light,

flourescent light of 3000-3200 lux were applied and set to 16 hour photoperiod. the number of

shoots, number of leaves, number of roots, shoots performance were observed weekly from 1

until 12 weeks after planting. Data analyzed with ANAVA non factorial and differences test with

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Medium Coconut

water (v/v)

Murashige Skoog (MS) Medium Coconut

water (v/v) GrowMore (GR)

0 ml M1C0 0 ml G1C0

50 ml M1C1 50 ml G1C1

100 ml M1C2 100 ml G1C2

150 ml M1C3 150 ml G1C3

200 ml M1C4 200 ml G1C4

250 ml M1C5 250 ml G1C5

Table 1. Combination of treatmen between MS medium with coconut water, Growmore

medium and coconut water

The first combinatios of medium is MS medium with coconut water:

MS + 0% coconut water (M1C0)

The second combination of medium is Growmore (GR) with coconut water:

GR + 0% coconut water (G1C0)

RESULTS AND DISCUSSION

Results of analysis of variance showed a significantly different effect (significant)

between the treatment given to the value of the all parameters: the number of shoots, number of

leaves and number of roots of the Dendrobium orchid, shoot performance.

Table 2. Number of Shoots, Leaves and Roots of Dendrobium Sp after Coconut Water

Treatment in MS Medium and Growmore medium

Combination treatment

Mean of shoot number Mean of leave number Mean of root number

Coconut water (%) MS Medium Growmore Medium MS Medium Growmore Medium MS Medium Growmore Medium

0 4.75 3 4 2.5 0.5 0

5 5.5 5.25 4.75 2.25 1 0

10 5.75 3.75 3.75 4 0.75 0

15 6.75 4 3.5 2.75 0 0

20 5.5 5.5 3.75 3.5 0 0

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50

a. Number of Shoot

Results showed that MS medium plus 15 % coconut water produces shoots grew

100%, the highest number of shoots (6.75). While on treatment with growmore, required a

concentration of 25% coconut water to produce the highest number of shoots (6.5). On

treatment with MS medium, it appears that the number of shoots increased with increasing

doses of coconut water until a certain dose (15%), but decreases with increasing doses of

coconut water (20%), with the number of shoots 5.5. On treatment with growmore, seen a trend

of increasing number of shoots, up to the highest dose given (25% coconut water) by the

number of shoots 6.5.

It is understandable that the nutrient content on MS medium is much more complete

than in the Growmore, so that the giving of 15% coconut water has stimulate the growth of

shoots. But with growmore added 25% coconut water also produces a high number of shoots.

This means that coconut water is potential to stimulate growth in the number of shoots on

Dendrobium orchids. Paramartha (2012) said that any effect of addition of NAA and BAP

combination on growth and development of Dendrobium taurulinum JJ Smith Seeds in vitro.

Rostika (2008) reported that the highest number of mangosteen axillary bud formation was

obtained on the medium containing 3 mg/l BA (Benzyl Adenine) . BA is one of cytokinines

hormone.

b. Number of Leaves

In the treatment of MS and with Growmore medium, it appears that the number of leaves

increased by giving low doses of coconut water (5% on MS medium and 10% in Growmore

medium), but decreases with increasing doses of coconut water. Surachman (2011) give the

coconut water for the in vitro propagation and increasing the Patchouli leaves number.

Figure 2. Number of Leaves of Dendrobium Sp after Coconut Water Treatment in MS Medium

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c. Number of Roots

In the treatment of MS medium with a low dose of coconut water (0-10%) can produce

roots (average 0.75), but with increasing doses of coconut water (15%), roots are not formed.

On treatment with growmore, the addition of coconut water did not produce roots.

1.

MS

2.

MS+ CW 5%

3.

MS+ CW 10 %

4.

MS+ CW 15 %

5.

MS+ CW 20 %

6.

MS+ CW 25 %

7.

GR

8.

Gr+ CW 5 %

9.

Gr+ CW 10 %

10.

Gr+ CW 15 %

11.

Gr+ CW 20 % 12. Gr+ CW 25 %

Figure 3. Performance of Dendrobium after 12 weeks planting. In the picture shows that the

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52

This study proved that coconut water can stimulate the increase in the number of

shoots, number of leaves, but can not stimulate the increase in the number of roots. Can be

understood as organic matter (plant growth regulator) contained in coconut water is a

cytokines, the role of potential to stimulate the growth of shoots not for improve the roots

(Harahap, 2011, 2012, Gunawan, 1997). Pisesha, (2008) said that any effect of concentration of

IAA, IBA and coconut water against root formation Poinsettia (Euphorbia pulcherrima Wild. Et.

Klotz) in vitro. IBA concentration and the period of pulse treatment have significant effects on the

average number of roots produced per shoot on Catharanthus roseus (Rupesh, 2013).

d. Performance of Shoots

Shoots performance was pale green shoots for giving of low doses of coconut water, will

be green with coconut water with medium doses, the higher the concentration of coconut water

will make the shoots appear stunted and eventually died.

Coconut water contains inorganic ions (chlorine, copper, iron, magnesium, phosphorus,

potassium, sodium, sulfur), nitrogen component (thanolamine and ammonia), amino acids and

related substances, enzymes, organic acids. In addition, coconut water also contains sugar

between 1.7 to 2.6% and protein from 0.07 to 0.55%. Besides being rich in minerals, coconut

water also contains a variety of vitamins such as citric acid, nicotinic acid, pantothenic acid, folic

acid, niacin, riboflavin, and thiamine.

The type and concentration of Plant Growth Regulator (PGR) was a component that

determines the success of tissue culture. The explants were cultured on MS medium,

Growmore medium, for further propagation, using coconut water was a class of cytokines that

role in cell division, stimulate the multiplication of shoots, stimulate DNA synthesis process

(Harahap, 2011)

CONCLUSION

There are significant effect of medium, coconut water to all parameters: the number of

shoots, number of leaves and number of roots of the Dendrobium orchid. Shoots performance

was pale green shoots for giving of low doses of coconut water, will be green with coconut water

with medium doses. Dendrobium needed medium doses (10-15%) coconut water for improve

the shoot number and the leave number. The higher the concentration of coconut water will

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REFERENCES

Amalia,. N.N,. (2013), Effect of Type and Vitamins Concentration on the Growth and Development of Dendrobium Seeds laxiflorum JJ Smith in In Vitro. Journal of Science and Arts POMITS, 1: 1-6

BPPP (Badan Penelitian dan Pengembangan Pertanian, (2004), Teknologi agribisni tanaman hias, Jakarta, Balai Penelitian Tanaman Hias. Pusat Penelitian dan Pengembanga Hortikultura.

Fahruroh, W., (2008), Effect of Growmore fertilizer (20:20:20) And Quick Grow of Adenium F

on Growth entres (Adenium obesum) After trimming, Bogor, Thesis, Faculty of

Agriculture, Bogor Agricultural University.

Harahap, (2011). Kultur Jaringan Tanaman. UNIMED Press. Medan.

Harahap (2012). Pertumbuhan Tunas Manggis In Vitro (Garcinia-Mangostana L.) Hasil

Perlakuan Zat Pengatur Tumbuh Benzyl Adenine dan Ukuran Eksplan yang Berbeda. Saintika Journal, Vol 12: 1-13.

Kartiman, R., (2004), Effect of combinations of growth regulators and pieces protocorm like

bodies for the propagation of the giant moon orchid (Phalaenopsis gigantea) with tissue

culture methods. Thesis, Faculty of Agriculture, Bogor Agricultural Institute.

Paramartha, A., EN, (2012), Effect of Concentration Addition PGR combination Naa And Bap on Growth and Development of Dendrobium Taurulinum JJ Smith Seeds In Vitro. Journal of Science and Arts of ITS, 1 (1):: 2301-928X.

Pisesha, (2008), Effect of IAA concentration, Iba, Bap, And Coconut Water Against Root Formation Poinsettia (Euphorbia pulcherrima Wild. Et. Klotz) In Vitro, Thesis, Faculty of Agriculture, Bogor Agricultural Institute.

Rostika I, Nopianti S, Ika M. (2008). Micropropagation of mangosteen (Garcinia-mangostana

L.). Indonesian Journal of Agriculture 1:28-33

Rupesh KR, Naresh KS, and Vandana S, (2013). Effect of plant growth regulators on

micropropagation of Catharanthus roseus. International Journal of Advanced

Biotechnology and Research 4:986-993.