Ppt Kfa Kromatografi Cair Kinerja Tinggi

Kromatograf Cair

Kromatograf Cair

Kinerja Tinggi (KCKT)

Kinerja Tinggi (KCKT)

KELOMPOK 16 :

KELOMPOK 16 :

NELLY MAYESTI MANALU

NELLY MAYESTI MANALU

SRIWULAN AYUNINGTYAS

SRIWULAN AYUNINGTYAS

D.III FA

D.III FA

Pengert!n

Pengert!n

Kr"#!t"gr!$ %!r Kner&!

Kr"#!t"gr!$ %!r Kner&! T

Tngg

ngg

'K%KT(HPL%)

'K%KT(HPL%)

Mer*+!,!n #et"-e !ng t-!, -estr*,t/

Mer*+!,!n #et"-e !ng t-!, -estr*,t/

-!n -!+!t -g*n!,!n 0!, *nt*, !n!ss

-!n -!+!t -g*n!,!n 0!, *nt*, !n!ss

,*!t!t/ -!n ,*!ntt!t/. te,n, +e#s!2!n

,*!t!t/ -!n ,*!ntt!t/. te,n, +e#s!2!n

!ng -ter#! se!r! *!s *nt*, !n!ss

!ng -ter#! se!r! *!s *nt*, !n!ss

-!n +e#*rn!n sen!4! tertent* -!!#

-!n +e#*rn!n sen!4! tertent* -!!#

s*!t* s!#+e -!!# 0er0!g! 0-!ng5

s*!t* s!#+e -!!# 0er0!g! 0-!ng5

!nt!r!

!n:

/!r#!s5

ng,*ng!n5

!nt!r!

!n:

/!r#!s5

ng,*ng!n5

0"te,n""g5 +"#er5 -!n n-*strn-*str

0"te,n""g5 +"#er5 -!n n-*strn-*str

#!,!n!n.

#!,!n!n.

SRIWUL

 Jenis - Jenis KCKT

(Kromatograf Cair Kinerja Tinggi)

matograf

dsorbsi

Kromatograf

Fase Terikat Kromatog

Penukar I

#!t"gr!$

s!ng!n I"n

Kr"#!t"gr!$

E,s*s U,*r!n

Kromatogr

Afnitas

Keuntungan dan kelebihan

KCKT

Kelebihan

HPL% -eng,!+ -eng!n !-!n! +"#+!5 P"#+! n #eng2!s,!n te,!n!n se,t!r 88 !t# *nt*, #eng!r,!n e*en -eng!n

te,!n!n tngg -!n ,"nst!n.

HPL% #e#, ,e*ngg*!n

,""#5 D#!n! ,""# HPL% n e02 +en-e, -!n e02 ,e.

,r"#!t"gr!# #en*n&*,,!n 2!s !ng --!+!t.

Kr"#!t"gr!# !ng -ngn,!n !-!!2 ,r"#!t"gr!# !ng *r*s tngg -eng!n e0!r se,e

#*ng,n.

Kekurangan

L!r*t!n 2!r*s -!r /!se -!#n! tere02 -!2** H!n! 0s! -g*n!,!n *nt*, !s!# "rg!n, H!r*s #enget!2* ,"#0n!s !ng "+t#*# !nt!r! +e!r*t5 !n!t5 -!n gr!-ent e*s H!rg!n! #!2! se2ngg! +engg*n!!nn! -!!# ng,*+ +enet!n !ng ter0!t!s

A!t Kr"#!t"gr!$ K%KT

Instr*#ent!s HPL% +!-! -!s!rn! ter-r !t!s: 1.4!-!2 /!se ger!, 'Reservoir )

<.P"#+!

3.!!t *nt*, #e#!s*,,!n s!#+e 'te#+!t n&e,s) .K""#

7.Dete,t"r

6.W!-!2 +en!#+*ng 0*!ng!n /!se ger!, -!n5 9.K"#+*ter !t!* ntegr!t"r !t!* +ere,!#.

L!n&*t!n...

1 !adah Fase "erak 

W!-!2 +e!r*t ,"s"ng !t!*+*n !0* !0"r!t"r*# -!+!t

-g*n!,!n se0!g! 4!-!2 /!se ger!,.

W!-!2 n 0!s!n! -!+!t

#en!#+*ng /!se ger!, !nt!r! 1 s!#+! < ter +e!r*t. F!se ger!, se0e*# -g*n!,!n 2!r*s -!,*,!n -egg!sng '+eng2!ng!n g!s) !ng !-! +!-! /!se ger!,. # Pom$a Pengg*n!!n +"#+! !t!* sste# +eng2!nt!r!n /!se ger!, !-!!2 *nt*, #en&!#n +r"ses

+eng2!nt!r!n /!se ger!, 0er!ngs*ng se!r! te+!t5 re+r"-*s0e5 ,"nst!n5 -!n 0e0!s -!r g!ngg*!n. Tg! &ens +"#+! !ng -g*n!,!n -!!# HPL% :  P"#+! reciprocating  P"#+! displacement   P"#+! pneumatic

%Tem$at Injeksi S!#+e !ng !,!n -#!s*,,!n ,e 0!g!n *&*ng ,""#5 2!r*s -eng!n -st*r0!ns !ng #n#*# -!r #!ter! ,""#. S!#+e !ng !,!n -+s!2,!n -#!s*,,!n ,e -!!# ,""# se!r! "t"#!ts !t!* #!n*! #e!* n&e,s. ="*#e n&e,s s!ng!t te+!t ,!ren! #e#+*n! s!#+e ""+ -eng!n >!r!0e

>"*#e '#s!n! <8 ? 788 @L). A-! tg! t+e -!s!r n&e,t"r !ng -!+!t -g*n!,!n : .St"+ "4 .Se+t*# .L""+ =!>e

&



Kolom

A-! < &ens ,""#

+!-! HPL% !t* ,""#

,"n>ens"n! -!n ,""#

#,r"0"r. K""# #er*+!,!n

0!g!n HPL% !ng #!n!

ter-!+!t /!se -!# *nt*,

0er!ngs*ngn! +r"ses

+e#s!2!n s"*t !t!* !n!t.

' etektor

Dete,t"r +!-! K%KT -,e"#+",,!n #en&!- < g""ng!n !t*: -ete,t"r *n>ers! '!ng #!#+* #en-ete,s B!t se!r! *#*#5 t-!, 0ers/!t s+es$,5 -!n t-!, 0ers/!t see,t/). Se+ert -ete,t"r n-e,s 0!s -!n -ete,t"r s+e,tr"#etr #!ss!C -!n g""ng!n -ete,t"r !ng s+es$, -!n see,t/5 se+ert -ete,t"r U==s5 -ete,t"r *"resens5 -!n ee,tr",#!. e0er!+! -ete,t"r !ng +!ng serng -g*n!,!n +!-! K%KT : !. Dete,t"r S+e,tr"/"t"#etr U==s

0. Dete,t"r photodiode-array 'PDA) . Dete,t"r *"resens

-. Dete,t"r n-e,s 0!s e. Dete,t"r ee,tr",#!

 Kom$uter* Intergrator atau

+ekorder

!!t

+eng*#+*

-!t!

se+ert

,"#+*ter5 ntegr!t"r5 !t!* re,"-er5

-2*0*ng,!n -eng!n -ete,t"r. A!t

n !,!n #eng*,*r sn! ee,tr"n,

!ng -2!s,!n "e2 -ete,t"r !*

#e#

 plot 

,!nn!

se0!g!

s*!t*

,r"#!t"gr!#

!ng

se!n&*tn!

-!+!t -e>!*!s "e2 se"r!ng !n!s

'+engg*n!).

A+,!s K%KT

e0er!+! !+,!s HPL% -!!# ,e2-*+!n :

•

HPL% -eng!n +rns+ ,r"#!t"gr!$ 0!n!, -g*n!,!n +!-!

n-*str /!r#!s -!n +ests-!.

•

!t B!t -eng!n ,e+"!r!n 0er0e-! !t* !nt!r! se-,t +"!r

s!#+! +"!r -!+!t -+s!2,!n -eng!n HPL% 0er-!s!r,!n

+!rts !r!r.

•

As!#!s!# n*,e!t -!+!t -+s!2,!n -eng!n ,""# +en*,!r

"n !ng -,"#0n!s,!n -eng!n ,""# 0*tr!n 0er!+s B!t

0er+"r

•

M"r$n5 2er"n -!n se#!!#n! te!2 -!+!t -+s!2,!n

-eng!n reBn +!SA

•

D!+!t #e#s!2,!n >t!#n>t!#n !ng !r*t -!!# !r.

•

Kr"#!t"gr!$ !r!n ,ner&! tngg 'K%KT(HPL%) -g*n!,!n

*nt*, #e#s!2,!n g""ng!ng""ng!n t!,!tsr

R*#*s Per2t*ng!n K%KT

,etode aku alam (Internal .tandard ,ethod)

/

D!r ,r"#!t"gr!# st!n-!r -!+!t -2t*ng res+"ns

/!,t"r re!t/ se0!g! 0er,*t :

r  Res+"ns /!,t"r re!t/

%  K"nsentr!s K"rn+"nen S!#+e

A  Le0!r !t!* Tngg P*n!, K"#+"nen S!#+e

%s  K"nsentr!s !,* D!!#

As

 Le0!r !t!* Tngg !,* D!!#

L!n&*t!n ...

D -!!# !#+*r!n s!#+e -g*n!,!n

r*#*s 0er,*t :

%*  K"nsentr!s ,"#+"nen s!#+e

A*

 Le0!r !t!* Tngg P*n!,

%s K"nsentr!s !,* D!!#

S"! Per2t*ng!n

• T!0et !n!get, !ng -0e - A+"t, +!-! et,etn! terter!

set!+ t!0et #eng!n-*ng 3<7 #g As+rn -!n 78 #g K!/en.

• Pr"se-*r Ker&! : A#0 -*! T!0et t!#0!2,!n 858993 g

Fen!setn ,"", -eng!n 18 # et!n" se!#! 18 #ent5 !n t!#0!2,!n 18 # !##"n*# /"r#!t 857 #" -#3 -!n

!#+*r!n n -ener,!n -eng!n /!se ger!, s!#+!t 188 #.  T!0et #eng!n-*ng 0!2!n0!2!n +e#0!4!5 #!,! !r*t!n n

2!r*s -s!rng se0e*# -,r"#!t"gr!$. Deng!n ,"n-s

+er"0!!n !ng -g*n!,!n ,etg! sen!4! terse0*t -!+!t -+s!2,!n -!!# 4!,t* se,t!r 3 #ent.

• D!,*,!n +en#0!ng!n St!n-!r -!r sen!4!sen!4! -

!t!s -!n -ener,!n se2ngg! ,"nsentr!sn! #en-e,!t ,"nsentr!s s!#+e -!n -n&e,s,!n ,e sste# ,r"#!t"gr!$ se0!n!, tg! ,!5 -+er"e2 -!t!-!t! se0!g!#!n!

ata hasil $erhitungan res$ons

0aktor relati0 As$irin dan Ka0ein

ata sam$el As$irin dan Ka0ein

dengan buku dalam Fenasetin

Ht*ng res+"ns /!,t"r re!t/5 ,!-!r As+rn -!n K!/en -!!#

t!0et. Pers!r!t!n F!r#!,"+e *nt*, As+rn -!nt!r! 7 J

-!n 187 J se-!ng,!n K!/en - !nt!r! 8 J -!n 118 J.

A+!,!2 t!0et terse0*t #e#en*2 +ers!r!t!n 

 !4!0 :

•

ntuk Injeksi 1

 :

K!-!r !s+rn

%*  17977  58  858993  856373 gr -!!# <

t!0et

1988

 8531967 gr -!!# 1

t!0et

K!-!r K!/en

%*  7863  5777  858993  85187 gr -!!# <

t!0et

1988

 8587<< gr -!!# 1 t!0et

• ntuk injeksi # / K!-!r !s+rn %*  17371  58  858993  8568 gr -!!# < t!0et 1619  853<< gr -!!# 1 t!0et K!-!r K!/en %*  9  5777  858993  85188 gr -!!# < t!0et 1619  8587<8 gr -!!# 1 t!0et

• Kadar rata2rata As$irin  8531967  853<<  8531 gr <

• Presentase As$irin  315  188J  5 J 3<7

• Kadar rata2rata Ka0ein  8587<<  8587<8  8587<1 gr <

• Persentase Ka0ein  7<5 1  188 J  185< J 78

er!rt ,!-!r As+rn -!n K!/en

Kesim$ulan

• K%KT #er*+!,!n te,n, !n!ss +e#s!2!n se,!g*s +enent*!n

,*!t!t/ #!*+*n ,*!ntt!t/ !ng 0!n!, -g*n!,!n +!-! sen!4!sen!4! !ng #e#+*n! tt, --2 tngg !ng t-!, -!+!t -!,*,!n -eng!n !n!ss se!r! ,r"#!t"gr!$ g!s.

• HPL% &*g! -!+!t -,e"#+",,!n 0er-!s!r,!n +!-! s/!t /!se

-!# -!n !t!* 0er-!s!r,!n +!-! #e,!ns#e s"r+s s"*t5 -eng!n &ens&ens HPL% se0!g! 0er,*t: ,r"#!t"gr!$ !-s"r0s5 ,r"#!t"gr!$ /!se ter,!t5 ,r"#!t"gr!$ +en*,!r "n5 ,r"#!t"gr!$ +!s!ng!n "n5 Kr"#!t"gr!$ e,s,*s *,*r!n -!n ,r"#!t"gr!$ !$nt!s.

• Ker&! HPL% +!-! +rns+n! !-!!2 +e#s!2!n !n!t ? !n!t

0er-!s!r,!n ,e+"!r!nn!5 !!tn! ter-r -!r ,""# 'se0!g! /!s! -!#) -!n !r*t!n tertent* se0!g! /!s! ger!,n!.

D!/t!r P*st!,!

A2#!-5 M. 11. Kromatograf Cair Kinerja Tinggi. S*r!0!!: Ar!ngg! Un>erst Press.

G!n-&!r5 I0n* G. <889. Kimia Farmasi Analisis. Y"g!,!rt!: P*st!,! Pe!&!r. Ke!e !n- H!nes5 P.. <88<. Analytical Chemistry . L"n-"n: IOS Sent$

P*0s2ers Lt-.

K2"+,!r5 S.M. <883. Konsep Dasar Kimia Analitik . !,!rt!: UI Press.

M*ns"n5 .W. 11. Analisis Farmasi Metode Modern. S*r!0!!: Un>erst!s Ar!ngg!.

Sette5 F. 19. and!ook o" #nstrumental Techni$ues "or Analytical Chemistry 5 Prente H! PTR5 Ne4 erse5 USA.

Un-er4""-5 D. <88<. Analisis Kimia Kuantitati" %disi &#. !,!rt!: Er!ngg!. *,!rn!en5 AK. 11. Kimia Analisa Kuantitati" . Y"g!,!rt!: De+!rte#en