Formulasi Krim Anti-Aging dari Konsentrat Sari Buah Stroberi (Fragaria x ananassa Duchesne ex Weston)

LAMPIRAN

Lampiran 2. Contoh surat pernyataan sukarelawan

SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM

PENELITIAN

Saya yang bertandatangan di bawah ini :

Nama

:

Umur

:

Alamat

:

Telah mendapat penjelasan secukupnya bahwa saya akan melakukan uji

krim anti-

aging

dari sari buah stroberi yang dipekatkan. Setelah mendapat

penjelasan secukupnya tentang manfaat penelitian ini dan efek sampingnya, maka

saya menyatakan SETUJU untuk ikut serta dalam penelitian dari Cut

Nuriramadhani

dengan judul “

FORMULASI KRIM ANTI-AGING

DARI KONSENTRAT SARI BUAH STROBERI (

Fragaria x

ananassa

Duchesne ex Weston)

”, sebagai usaha untuk mengetahui apakah

sediaan krim anti-

aging

dari sari buah stroberi yang dipekatkan yang dihasilkan

mampu atau tidak dalam memulihkan kulit yang telah mengalami penuaan. Saya

menyatakan sukarela dan bersedia untuk mengikuti prosedur penelitian yang telah

ditetapkan.

Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari

pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan

sebagaimana mestinya.

Medan, November 2014

Peneliti,

Sukarelawan,

Lampiran 3. Gambar tanaman stroberi dan buah stroberi

A

B

Keterangan:

Lampiran 6. Skema pembuatan krim

Ditimbang

Dilebur di atas penangas air Dilarutkan dalam beaker

pada suhu 70 -

75˚C

glass pada suhu 70˚C

Dimasukkan dalam lumpang

panas diaduk konstan

Diuji pH

Diuji tipe emulsi

Ditimbang masing-masing konsentrasi

Digerus dalam lumpang

Ditambahkan dasar krim sedikit demi sedikit

hingga homogen

Diuji homogenitas

Diuji pH

Diuji tipe emulsi

Diuji stabilitas selama 12 minggu

Bahan-bahan

Fase air:

Nipagin

Propilen glikol

Natrium edetat

Trietanol amin

Aquadest

Fase minyak:

Vaselin

Asam stearat

Gliseril monostearat

Setil alkohol

Dasar krim

Hasil dasar

krim

Sari buah stroberi

yang dipekatkan

Lampiran 7. Gambar sediaan setelah dibuat dan setelah 12 minggu

A

B

Keterangan:

A

: Gambar sediaan setelah dibuat

B

: Gambar sediaan setelah 12 minggu

5%

7,5%

Blanko

10%

Lampiran 8. Gambar hasil uji homogenitas dan hasil penentuan tipe emulsi

A

B

Keterangan:

A

: Gambar hasil uji homogenitas

B

: Gambar hasil penentuan tipe emulsi

Lampiran 9. Gambar alat

skin analyzer

(Aramo-SG) dan

moisture checker

(Aramo-SG)

A

B

Keterangan:

Lampiran 11. Kriteria kondisi kulit sukarelawan sebelum pemakaian krim

Sukarelawan

Usia

(thn)

Kriteria kondisi kulit sukarelawan sebelum

pemakaian krim

Kadar air Kehalusan

Pori

Banyaknya

noda

Keriput

1

23

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

2

24

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

3

24

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

4

24

Dehidrasi

Normal

Sangat

besar

Banyak

noda

Berkeriput

5

23

Dehidrasi

Normal

Sangat

besar

Banyak

noda

Berkeriput

6

26

Dehidrasi

Normal

Sangat

besar

Banyak

noda

Berkeriput

7

24

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

8

30

Dehidrasi

Normal

Sangat

besar

Banyak

noda

Berkeriput

9

28

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

10

25

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

parah

11

30

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

parah

12

25

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

parah

13

24

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

14

24

Dehidrasi

Normal

Beberapa

besar

Banyak

noda

Berkeriput

15

24

Dehidrasi

Normal

Beberapa

besar

Banyak

Lampiran 12. Hasil pengukuran menggunakan

skin analyzer



Kadar air

Kondisi awal:

Pemakaian 1 minggu:

Pemakaian 2 minggu:

Lampiran 12. (Lanjutan)

Pemakaian 4 minggu:



Kehalusan dan pori

Lampiran 12. (Lanjutan)

Pemakaian 1 minggu:

Lampiran 12. (Lanjutan)

Pemakaian 3 minggu:

Lampiran 12. (Lanjutan)



Banyaknya noda

Kondisi awal:

Lampiran 12. (Lanjutan)

Pemakaian 2 minggu:

Lampiran 12. (Lanjutan)

Pemakaian 4 minggu:



Keriput dan kedalaman keriput

Lampiran 12. (Lanjutan)

Pemakaian 1 minggu:

Lampiran 12. (Lanjutan)

Pemakaian 3 minggu:

Kelembaban

One-Sample Kolmogorov-Smirnov Test

kondisi_awal minggu_1 minggu_2 minggu_3 minggu_4

N 15 15 15 15 15

Normal Parametersa

Mean 28.5333 30.6000 32.0000 33.4000 34.6000 Std. Deviation .74322 1.24212 1.73205 1.91982 2.19740 Most Extreme

Differences

Absolute .268 .219 .185 .151 .148 Positive .230 .219 .185 .116 .148 Negative -.268 -.137 -.185 -.151 -.126 Kolmogorov-Smirnov Z 1.039 .847 .716 .584 .574 Asymp. Sig. (2-tailed) .230 .469 .685 .885 .896

a. Test distribution is Normal.

ANOVA

Sum of Squares df Mean Square F Sig.

kondisi_awal Between Groups 1.733 4 .433 .722 .596

Within Groups 6.000 10 .600

Total 7.733 14

minggu_1 Between Groups 15.600 4 3.900 6.500 .008

Within Groups 6.000 10 .600

Total 21.600 14

minggu_2 Between Groups 35.333 4 8.833 13.250 .001

Within Groups 6.667 10 .667

Total 42.000 14

minggu_3 Between Groups 45.600 4 11.400 19.000 .000

Within Groups 6.000 10 .600

Total 51.600 14

minggu_4 Between Groups 62.267 4 15.567 29.187 .000

Within Groups 5.333 10 .533

Total 67.600 14

minggu_1 Tukey HSD

perawatan N

Subset for alpha = 0.05

1 2

f1 3 29.3333 f0 3 30.0000

f3 3 30.3333 30.3333 f2 3 31.0000 31.0000

f4 3 32.3333

Sig. .136 .061 Means for groups in homogeneous subsets are displayed.

minggu_2 Tukey HSD

perawatan N

Subset for alpha = 0.05

1 2 3

f0 3 30.0000

f1 3 30.6667 30.6667

f2 3 32.3333 32.3333 f3 3 32.6667 32.6667

f4 3 34.3333

Sig. .850 .078 .078 Means for groups in homogeneous subsets are displayed.

minggu_3 Tukey HSD

perawatan N

Subset for alpha = 0.05

1 2 3

f0 3 30.6667

f1 3 32.6667 32.6667 f3 3 33.6667

f2 3 34.0000 34.0000

f4 3 36.0000

minggu_4 Tukey HSD

perawatan N

Subset for alpha = 0.05

1 2 3 4

f0 3 31.6667

f1 3 33.3333 33.3333

f2 3 34.6667 34.6667

f3 3 35.6667

f4 3 37.6667

Sig. .107 .242 .487 1.000 Means for groups in homogeneous subsets are displayed.

Kehalusan

One-Sample Kolmogorov-Smirnov Test

kondisi_awal minggu_1 minggu_2 minggu_3 minggu_4

N 15 15 15 15 15

Normal Parametersa Mean 41.4667 38.0667 35.2667 33.8667 30.8000 Std. Deviation 2.09989 3.51460 2.91466 3.94365 6.16673 Most Extreme

Differences

Absolute .255 .138 .270 .220 .160 Positive .255 .135 .270 .220 .160 Negative -.154 -.138 -.109 -.118 -.135 Kolmogorov-Smirnov Z .986 .535 1.045 .851 .619 Asymp. Sig. (2-tailed) .285 .937 .225 .463 .838 a. Test distribution is Normal.

ANOVA

Sum of Squares df Mean Square F Sig. kondisi_awal Between Groups 29.733 4 7.433 2.323 .128

Within Groups 32.000 10 3.200 Total 61.733 14

minggu_1 Between Groups 80.933 4 20.233 2.199 .142 Within Groups 92.000 10 9.200

Total 172.933 14

minggu_2 Between Groups 58.267 4 14.567 2.401 .119 Within Groups 60.667 10 6.067

Total 118.933 14

minggu_3 Between Groups 140.400 4 35.100 4.539 .024 Within Groups 77.333 10 7.733

Total 217.733 14

minggu_4 Between Groups 310.400 4 77.600 3.495 .049 Within Groups 222.000 10 22.200

Total 532.400 14

kondisi_awal

Tukey HSD

perawatan_ kehalusan N

Subset for alpha = 0.05

1 f1 3 39.6667 f2 3 40.0000 f4 3 42.0000 f0 3 42.3333 f3 3 43.3333

Sig. .164

minggu_1

Tukey HSD

perawatan_ kehalusan N

Subset for alpha = 0.05

1 f1 3 35.6667 f2 3 36.3333 f3 3 38.0000 f4 3 38.0000 f0 3 42.3333

Sig. .125

Means for groups in homogeneous subsets are displayed.

minggu_2

Tukey HSD

perawatan_ kehalusan N

Subset for alpha = 0.05

1 f1 3 33.6667 f2 3 33.6667 f4 3 34.6667 f3 3 35.3333 f0 3 39.0000

Sig. .133

Means for groups in homogeneous subsets are displayed.

minggu_3

Tukey HSD

perawatan_ kehalusan N

Subset for alpha = 0.05

1 2

f2 3 30.6667

f1 3 32.3333 32.3333 f3 3 33.3333 33.3333 f4 3 33.3333 33.3333

f0 3 39.6667

Sig. .765 .055 Means for groups in homogeneous subsets are displayed.

minggu_4

Tukey HSD

perawatan_ kehalusan N

Subset for alpha = 0.05

1 2

f3 3 26.6667

f1 3 29.0000 29.0000 f4 3 29.0000 29.0000 f2 3 29.6667 29.6667

f0 3 39.6667

Sig. .931 .111 Means for groups in homogeneous subsets are displayed.

Besar pori

One-Sample Kolmogorov-Smirnov Test

kondisi_

awal minngu_1 minggu_2 minggu_3 minggu_4

N 15 15 15 15 15

Normal Parametersa Mean 39.1333 35.8667 32.2667 29.6667 25.0000 Std. Deviation 5.75533 7.63326 9.52040 9.46925 10.16998 Most Extreme

Differences

ANOVA

Sum of Squares df Mean Square F Sig. kondisi_awal Between Groups 349.733 4 87.433 7.670 .004

Within Groups 114.000 10 11.400 Total 463.733 14

minngu_1 Between Groups 635.733 4 158.933 8.830 .003 Within Groups 180.000 10 18.000

Total 815.733 14

minggu_2 Between Groups 1146.267 4 286.567 23.361 .000 Within Groups 122.667 10 12.267

Total 1268.933 14

minggu_3 Between Groups 1115.333 4 278.833 19.917 .000 Within Groups 140.000 10 14.000

Total 1255.333 14

minggu_4 Between Groups 1303.333 4 325.833 22.523 .000 Within Groups 144.667 10 14.467

Total 1448.000 14

kondisi_awal

Tukey HSD

perawatan_ besar pori N

Subset for alpha = 0.05

1 2

f3 3 34.3333 f0 3 35.6667 f4 3 37.6667

f2 3 40.0000 40.0000

f1 3 48.0000

Sig. .309 .091 Means for groups in homogeneous subsets are displayed.

minggu_1

Tukey HSD

perawatan_ besar pori N

Subset for alpha = 0.05

1 2

f4 3 28.0000 f3 3 30.3333

f0 3 35.6667 35.6667 f2 3 39.0000 39.0000

f1 3 46.3333

Sig. .060 .069 Means for groups in homogeneous subsets are displayed.

minggu_2

Tukey HSD

perawatan_ besar pori N

Subset for alpha = 0.05

1 2 3

f4 3 18.6667

f3 3 26.6667 26.6667

f0 3 35.6667 35.6667

f2 3 36.3333

f1 3 44.0000

Sig. .107 .062 .089 Means for groups in homogeneous subsets are displayed.

minggu_3

Tukey HSD perawat an_besa

rpori N

Subset for alpha = 0.05

1 2 3

f4 3 17.3333

f3 3 23.0000 23.0000

f2 3 32.3333 32.3333

f0 3 33.6667

f1 3 42.0000

minggu_4

Tukey HSD

perawatan_ besar pori N

Subset for alpha = 0.05

1 2 3 4

f4 3 10.6667

f3 3 20.0000 20.0000

f2 3 24.3333 24.3333

f0 3 33.6667 33.6667

f1 3 36.3333

Sig. .078 .644 .078 .906 Means for groups in homogeneous subsets are displayed.

Banyaknya noda

One-Sample Kolmogorov-Smirnov Test

kondisi_

awal minggu_1 minggu_2 minggu_3 minggu_4

N 15 15 15 15 15

Normal Parametersa Mean 52.7333 47.5333 42.7333 36.7333 32.5333 Std. Deviation 5.03511 4.82355 6.57340 8.61449 11.25590 Most Extreme

Differences

Absolute .146 .167 .188 .134 .154 Positive .146 .167 .144 .134 .154 Negative -.091 -.165 -.188 -.105 -.115 Kolmogorov-Smirnov Z .564 .647 .730 .519 .597 Asymp. Sig. (2-tailed) .908 .797 .661 .951 .868 a. Test distribution is Normal.

ANOVA

Sum of Squares df Mean Square F Sig. kondisi_awal Between Groups 94.267 4 23.567 .904 .497

Within Groups 260.667 10 26.067 Total 354.933 14

minggu_1 Between Groups 99.067 4 24.767 1.093 .411 Within Groups 226.667 10 22.667

Total 325.733 14

minggu_2 Between Groups 390.267 4 97.567 4.545 .024 Within Groups 214.667 10 21.467

Total 604.933 14

minggu_3 Between Groups 885.600 4 221.400 14.439 .000 Within Groups 153.333 10 15.333

Total 1038.933 14

minggu_4 Between Groups 1673.067 4 418.267 41.550 .000 Within Groups 100.667 10 10.067

Total 1773.733 14

kondisi_awal

Tukey HSD

perawatan_ banyak

noda N

Subset for alpha = 0.05

1 f1 3 49.3333 f0 3 50.3333 f2 3 53.6667 f4 3 54.3333 f3 3 56.0000

Sig. .529

minggu_1

Tukey HSD

perawatan_ banyak

noda N

Subset for alpha = 0.05

1 f4 3 43.0000 f1 3 47.3333 f2 3 47.3333 f3 3 49.6667 f0 3 50.3333

Sig. .382

Means for groups in homogeneous subsets are displayed.

minggu_2

Tukey HSD perawatan_ banyak

noda N

Subset for alpha = 0.05

1 2

f4 3 34.3333

f3 3 42.0000 42.0000 f2 3 43.3333 43.3333 f1 3 43.6667 43.6667

f0 3 50.3333

Sig. .175 .253 Means for groups in homogeneous subsets are displayed.

minggu_3

Tukey HSD perawatan_ banyak

noda N

Subset for alpha = 0.05

1 2 3

f4 3 26.3333

f3 3 34.0000 34.0000 f2 3 35.3333 35.3333 f1 3 38.0000

f0 3 50.0000

Sig. .104 .724 1.000 Means for groups in homogeneous subsets are displayed.

minggu_4

Tukey HSD perawatan_ banyak

noda N

Subset for alpha = 0.05

1 2 3 4

f4 3 19.3333

f3 3 25.3333 25.3333

f2 3 30.6667 30.6667

f1 3 37.3333

f0 3 50.0000

Sig. .217 .307 .149 1.000 Means for groups in homogeneous subsets are displayed.

Keriput

One-Sample Kolmogorov-Smirnov Test

kondisi_

awal minggu_1 minggu_2 minggu_3 minggu_4

N 15 15 15 15 15

Normal Parametersa Mean 47.4000 41.7333 33.8000 26.8667 22.7333 Std. Deviation 5.74208 4.89120 8.36831 11.03803 13.04096 Most Extreme

Differences

ANOVA

Sum of Squares df Mean Square F Sig. kondisi_awal Between Groups 372.267 4 93.067 10.418 .001

Within Groups 89.333 10 8.933 Total 461.600 14

minggu_1 Between Groups 162.267 4 40.567 2.349 .125 Within Groups 172.667 10 17.267

Total 334.933 14

minggu_2 Between Groups 644.400 4 161.100 4.795 .020 Within Groups 336.000 10 33.600

Total 980.400 14

minggu_3 Between Groups 1246.400 4 311.600 6.784 .007 Within Groups 459.333 10 45.933

Total 1705.733 14

minggu_4 Between Groups 2272.267 4 568.067 52.276 .000 Within Groups 108.667 10 10.867

Total 2380.933 14

kondisi_awal

Tukey HSD

perawatan_ keriput N

Subset for alpha = 0.05

1 2

f0 3 43.0000 f4 3 43.3333 f2 3 46.0000 f1 3 48.0000

f3 3 56.6667

Sig. .311 1.000 Means for groups in homogeneous subsets are displayed.

minggu_1

Tukey HSD

perawatan_ keriput N

Subset for alpha = 0.05

1 f4 3 35.6667 f2 3 41.3333 f0 3 43.0000 f1 3 43.3333 f3 3 45.3333

Sig. .099

Means for groups in homogeneous subsets are displayed.

minggu_2

Tukey HSD

perawatan_ keriput N

Subset for alpha = 0.05

1 2

f4 3 22.6667

f3 3 32.6667 32.6667 f2 3 35.0000 35.0000 f1 3 35.6667 35.6667

f0 3 43.0000

Sig. .115 .260 Means for groups in homogeneous subsets are displayed.

minggu_3

Tukey HSD

perawatan_ keriput N

Subset for alpha = 0.05

1 2

f4 3 15.0000 f3 3 23.6667

f2 3 25.0000 25.0000 f1 3 27.6667 27.6667

f0 3 43.0000

minggu_4

Tukey HSD

perawatan_ keriput N

Subset for alpha = 0.05

1 2 3

f4 3 5.3333

f3 3 16.6667 f2 3 23.3333 f1 3 25.3333

f0 3 43.0000

Sig. 1.000 .056 1.000 Means for groups in homogeneous subsets are displayed.

Kedalaman keriput

One-Sample Kolmogorov-Smirnov Test

kondisi_awal minggu_1 minggu_2 minggu_3 minggu_4

N 15 15 15 15 15

Normal Parametersa

Mean 49.6667 45.0000 41.0000 35.6667 33.4667

Std. Deviation 10.83425 11.43928 11.94033 11.75139 11.51314

Most Extreme Differences

Absolute .258 .202 .133 .133 .163

Positive .258 .202 .133 .133 .163

Negative -.134 -.124 -.105 -.091 -.090

Kolmogorov-Smirnov Z .999 .784 .513 .515 .630

Asymp. Sig. (2-tailed) .271 .571 .955 .954 .822

a. Test distribution is Normal.

ANOVA

Sum of Squares df Mean Square F Sig.

kondisi_awal Between Groups 785.333 4 196.333 2.288 .132

Within Groups _{858.000 10 85.800}

Total _{1643.333 14}

minggu_1 Between Groups 1044.667 4 261.167 3.317 .056

Within Groups _{787.333 10 78.733}

Total _{1832.000 14}

minggu_2 Between Groups 1295.333 4 323.833 4.622 .023

Within Groups _{700.667 10 70.067}

Total _{1996.000 14}

minggu_3 Between Groups 1174.667 4 293.667 3.871 .038

Within Groups _{758.667 10 75.867}

Total _{1933.333 14}

minggu_4 Between Groups 989.067 4 247.267 2.853 .081

Within Groups _{866.667 10 86.667}

Total _{1855.733 14}

kondisi_awal

Tukey HSD

perawatan_ kedalaman keriput N

Subset for alpha = 0.05

1

f4 3 42.0000 f3 3 43.0000 f1 3 47.3333 f0 3 55.3333 f2 3 60.6667

Sig. .174

minggu_1

Tukey HSD

perawatan_ kedalaman keriput N

Subset for alpha = 0.05

1 f3 3 33.6667 f4 3 37.6667 f1 3 45.6667 f2 3 52.6667 f0 3 55.3333

Sig. .080

Means for groups in homogeneous subsets are displayed.

minggu_2

Tukey HSD

perawatan_ kedalaman keriput N

Subset for alpha = 0.05

1 2

f3 3 27.3333

f4 3 33.6667 33.6667 f1 3 43.6667 43.6667 f2 3 47.3333 47.3333

f0 3 53.0000

Sig. .088 .102 Means for groups in homogeneous subsets are displayed.

minggu_3

Tukey HSD perawatan_ kedalaman keriput N

Subset for alpha = 0.05

1 2

f3 3 24.0000

f4 3 28.0000 28.0000 f1 3 38.0000 38.0000 f2 3 39.3333 39.3333

f0 3 49.0000

Sig. .270 .084 Means for groups in homogeneous subsets are displayed.

Lampiran 13. (Lanjutan)

minggu_4

perawatan_ kedalaman keriput N

Subset for alpha = 0.05

1 f3 3 22.3333 f4 3 27.0000 f2 3 34.0000 f1 3 39.0000 f0 3 45.0000

Sig. .081