Lampiran 2.
Surat pernyataan persetujuan ikut serta dalam penelitian
SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM
PENELITIAN
Saya yang bertandatangan di bawah ini :
Nama
:
Umur
:
Alamat
:
Telah mendapat penjelasan secukupnya bahwa saya akan melakukan uji
krim ekstrak kulit buah delima terhadap kulit sebagai sediaan krim anti-aging.
Setelah mendapat penjelasan secukupnya tentang manfaat penelitian ini dan efek
sampingnya, maka saya menyatakan
SETUJU
untuk ikut serta dalam penelitian
dari
Nailul Ramadhilla dengan judul “FORMULASI DAN UJI EFEK ANTI
-AGING DARI KRIM MENGANDUNG EKSTRAK KULIT BUAH DELIMA
(Punica granatum L), sebagai usaha untuk mengetahui apakah sediaan krim
anti-aging dari ekstrak kulit buah delima mampu atau tidak dalam memulihkan kulit
yang telah mengalami penuaan. Saya menyatakan sukarela dan bersedia untuk
mengikuti prosedur penelitian yang telah ditetapkan.
Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari
pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan
sebagaimana mestinya.
Medan, 26 November 2014
Peneliti,
Sukarelawan,
Lampiran 3.
Gambar sampel
A
Lampiran 3.
(Lanjutan)
C
D
Keterangan: A: Buah delima
Lampiran 4.
Gambar alat
A B
C
Keterangan:
Lampiran 5.
Gambar sediaan krim
Krim sediaan setelah dibuat
Keterangan:
A : Krim A (blanko)
B : Krim B (ekstrak kulit buah delima 2,5%)
C : Krim C (ekstrak kulit buah delima 5%)
D : Krim D (ekstrak kulit buah delima 7,5%)
E
: Krim E (ekstrak kulit buah delima 10%)
Lampiran 6.
Gambar hasil uji evaluasi sediaan krim
A
B
Keterangan:
A: Gambar uji homogenitas sediaan
B: Gambar uji tipe emulsi sediaan
Blanko Krim 2,5% Krim 5% Krim 7,5% Krim 10%
Lampiran 7.
Gambar sediaan krim yang dibuat pada awal pembuatan dan gambar
krim penyimpanan 12 minggu
Gambar krim awal pembuatan
Gambar krim minggu ke 12
Keterangan:
A : Krim A (blanko)
B : Krim B (ekstrak kulit buah delima 2,5%)
C : Krim C (ekstrak kulit buah delima 5%)
D : Krim D (ekstrak kulit buah delima 7,5%)
E
: Krim E (ekstrak kulit buah delima 10%)
C
D
E
B
A
E
D
C
B
Lampiran 8.
Bagan Alir Proses Ekstraksi kulit buat delima
Diiris, dikeringkan dilemari
pengering (suhu 40-50
oC), lalu
dihaluskan
Dimasukkan kedalam bejana
Ditambahkan pelarut etanol 70%
3,75L
Ditutup dengan penutup wadah dan
kemudian didiamkan selama 5 hari
Diaduk sehari sekali
Setelah 5 hari maserat disaring
dengan kain kemudian pada ampas
tambahkan cairan penyari 1,25 L
kemudiaan dimasukkan ke dalam
jerigen
Diuapkan dengan alat rotary
evaporator
500 g kulit buah
delima
Maserat kulit buah
delima
Lampiran 9.
Bagan Alir Proses Pembuatan Sediaan Krim Anti-aging
Ditimbang 12 gram asam stearat
5 gram sorbitol
Ditimbang 0,5 gram setil alkohol
1gram trietanolamin
Dimasukkan kedalam cawan
0,2 gram nipagin
penguap
ad akuades
Dilebur di atas penangas air
Dilarutkan dengan air
panas yang telah ditakar
pada suhu 70
0C
Digerus konstan di dalam
Ekstrak kulit buah
lumpang panas hingga
delima ditambahkan
terbentuk massa krim
3 g propilen glikol
digerus sampai homogen
Massa II ditambahkan Massa I
digerus sampai homogen
Ditambahkan 3 tetes pewangi
Fase air
Fase minyak
Fase minyak + fase air
Massa I
(Dasar krim)
Bahan aktif
Massa II
Lampiran 10.
Gambar hasil pengukuran menggunakan skin analyzer
Kadar air
Kondisi awal
Pemulihan ke-1
Pemulihan ke-2
Pemulihan ke-3
Lampiran 10.
(Lanjutan)
Kehalusan (Evennes) dan pori (pore)
Kondisi awal
Pemulihan ke-1
Lampiran 10.
(Lanjutan)
Pemulihan ke-3
Lampiran 10.
(Lanjutan)
Noda (spot)
Kondisi awal
Lampiran 10.
(Lanjutan)
Pemulihan ke-2
Lampiran 10.
(Lanjutan)
Lampiran 10.
(
Lanjutan)
Keriput (Wrinkle)
Kondisi awal
Lampiran 10.
(Lanjutan)
Pemulihan ke-2
Lampiran 10.
(Lanjutan)
Lampiran 11.
Data hasil uji statistik
Kelembaban (Moisture)
ANOVA
Sum of Squares df Mean Square F Sig.
Sebelum Between Groups 11.611 5 2.322 1.548 .247
Within Groups 18.000 12 1.500
Total 29.611 17
MingguI Between Groups 28.667 5 5.733 3.559 .033
Within Groups 19.333 12 1.611
Total 48.000 17
MingguII Between Groups 51.611 5 10.322 10.322 .001
Within Groups 12.000 12 1.000
Total 63.611 17
MingguIII Between Groups 87.611 5 17.522 10.513 .000
Within Groups 20.000 12 1.667
Total 107.611 17
MingguIV Between Groups 126.444 5 25.289 65.029 .000
Within Groups 4.667 12 .389
Total 131.111 17
Sebelum
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula A 3 26.33
Formula B 3 27.33
Formula C 3 27.67
Formula D 3 27.67
Formula E 3 28.67
Formula F 3 28.67
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguI
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula A 3 28.33
Formula B 3 29.33 29.33
Formula C 3 30.33 30.33
Formula D 3 30.33 30.33
Formula E 3 31.67 31.67
Formula F 3 32.00
Sig. .063 .178
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguII
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula A 3 29.67
Formula B 3 31.00 31.00
Formula C 3 31.33 31.33 31.33
Formula F 3 33.67 33.67
Formula D 3 34.00
Formula E 3 34.00
Sig. .376 .058 .058
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguIII
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula A 3 29.67
Formula B 3 31.67 31.67
Formula C 3 32.67 32.67
Formula D 3 34.67 34.67
Formula E 3 34.67 34.67
Formula F 3 36.33
Sig. .116 .116 .624
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguIV
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula A 3 30.00
Formula B 3 32.00
Formula C 3 33.33
Formula D 3 36.33
Formula E 3 36.33
Formula F 3 37.33
Sig. 1.000 .166 .413
Means for groups in homogeneous subsets are displayed.
Lampiran
11.
(Lanjutan)
Kehalusan (Evennes)
ANOVA
Sum of Squares df Mean Square F Sig.
Sebelum Between Groups 171.611 5 34.322 5.566 .007
Within Groups 74.000 12 6.167
Total 245.611 17
MingguI Between Groups 114.444 5 22.889 2.382 .101
Within Groups 115.333 12 9.611
Total 229.778 17
MingguII Between Groups 119.833 5 23.967 2.201 .122
Within Groups 130.667 12 10.889
Total 250.500 17
MingguIII Between Groups 142.944 5 28.589 3.431 .037
Within Groups 100.000 12 8.333
Total 242.944 17
MingguIV Between Groups 208.278 5 41.656 4.600 .014
Within Groups 108.667 12 9.056
Total 316.944 17
Sebelum
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula F 3 35.33
Formula E 3 36.67
Formula D 3 37.00 37.00
Formula C 3 41.00 41.00
Formula B 3 42.00 42.00
Formula A 3 43.67
Sig. .056 .056
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguI
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 35.00
Formula E 3 36.00
Formula D 3 36.67
Formula C 3 37.00
Formula B 3 41.00
Formula A 3 41.67
Sig. .162
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguII
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 33.33
Formula E 3 33.33
Formula D 3 36.67
Formula C 3 37.00
Formula B 3 38.00
Formula A 3 40.67
Sig. .141
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguIII
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula F 3 32.00
Formula E 3 32.00
Formula D 3 34.67 34.67
Formula C 3 35.33 35.33
Formula B 3 36.00 36.00
Formula A 3 40.33
Sig. .558 .229
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguIV
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula F 3 30.00
Formula E 3 30.00
Formula D 3 34.00 34.00
Formula C 3 34.67 34.67
Formula B 3 35.00 35.00
Formula A 3 40.00
Sig. .379 .216
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
Pori (pore)
ANOVA
Sum of Squares df Mean Square F Sig.
Sebelum Between Groups 912.667 5 182.533 2.237 .118
Within Groups 979.333 12 81.611
Total 1892.000 17
MingguI Between Groups 139.167 5 27.833 1.580 .239
Within Groups 211.333 12 17.611
Total 350.500 17
MingguII Between Groups 33.778 5 6.756 1.013 .451
Within Groups 80.000 12 6.667
Total 113.778 17
MingguIII Between Groups 213.611 5 42.722 1.529 .253
Within Groups 335.333 12 27.944
Total 548.944 17
MingguIV Between Groups 506.944 5 101.389 14.600 .000
Within Groups 83.333 12 6.944
Total 590.278 17
Sebelum
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 21.33
Formula E 3 26.33
Formula D 3 26.67
Formula C 3 31.00
Formula B 3 35.33
Formula A 3 43.33
Sig. .093
MingguI
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 20.67
Formula E 3 23.67
Formula D 3 24.33
Formula C 3 26.00
Formula B 3 26.67
Formula A 3 29.67
Sig. .164
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguII
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 19.33
Formula E 3 20.00
Formula D 3 20.00
Formula C 3 20.67
Formula B 3 22.00
Formula A 3 23.33
Sig. .448
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 11.
(Lanjutan)
Lampiran 11.
(Lanjutan)
MingguIII
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 12.00
Formula E 3 15.67
Formula D 3 18.67
Formula C 3 18.67
Formula B 3 20.67
Formula A 3 22.67
Sig. .207
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguIV
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula F 3 7.00
Formula E 3 9.33 9.33
Formula D 3 13.33 13.33
Formula C 3 14.67 14.67
Formula B 3 20.67
Formula A 3 21.33
Sig. .099 .205 .077
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
Noda (spot)
ANOVA
Sum of Squares df Mean Square F Sig.
Sebelum Between Groups 20.667 5 4.133 2.566 .084
Within Groups 19.333 12 1.611
Total 40.000 17
MingguI Between Groups 33.833 5 6.767 3.929 .024
Within Groups 20.667 12 1.722
Total 54.500 17
MingguII Between Groups 45.833 5 9.167 8.684 .001
Within Groups 12.667 12 1.056
Total 58.500 17
MingguIII Between Groups 83.611 5 16.722 13.682 .000
Within Groups 14.667 12 1.222
Total 98.278 17
MingguIV Between Groups 119.111 5 23.822 10.459 .000
Within Groups 27.333 12 2.278
Total 146.444 17
Sebelum
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 25.33
Formula E 3 26.00
Formula D 3 26.33
Formula C 3 26.33
Formula B 3 27.33
Formula A 3 28.67
Sig. .063
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguI
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula F 3 25.00
Formula E 3 25.00
Formula D 3 25.33 25.33
Formula C 3 25.67 25.67
Formula B 3 27.33 27.33
Formula A 3 28.67
Sig. .314 .076
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguII
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula F 3 24.00
Formula E 3 24.33
Formula D 3 24.67
Formula C 3 25.00
Formula B 3 26.33 26.33
Formula A 3 28.67
Sig. .129 .129
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguIII
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula F 3 22.33
Formula E 3 23.00
Formula D 3 23.33
Formula C 3 24.00 24.00
Formula B 3 26.67 26.67
Formula A 3 28.33
Sig. .475 .098 .475
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguIV
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula F 3 20.00
Formula E 3 21.33
Formula D 3 22.67 22.67
Formula C 3 23.33 23.33
Formula B 3 25.67 25.67
Formula A 3 27.67
Sig. .145 .219 .600
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
Keriput (Wrinkle)
ANOVA
Sum of Squares df Mean Square F Sig.
Sebelum Between Groups 551.611 5 110.322 3.162 .047
Within Groups 418.667 12 34.889
Total 970.278 17
MingguI Between Groups 850.944 5 170.189 6.176 .005
Within Groups 330.667 12 27.556
Total 1181.611 17
MingguII Between Groups 1065.778 5 213.156 9.788 .001
Within Groups 261.333 12 21.778
Total 1327.111 17
MingguIII Between Groups 1429.111 5 285.822 11.233 .000
Within Groups 305.333 12 25.444
Total 1734.444 17
MingguIV Between Groups 1983.611 5 396.722 16.454 .000
Within Groups 289.333 12 24.111
Total 2272.944 17
Sebelum
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 26.00
Formula E 3 33.67
Formula D 3 35.33
Formula C 3 40.67
Formula B 3 41.00
Formula A 3 41.67
Sig. .060
Lampiran 11.
(Lanjutan)
a. Uses Harmonic Mean Sample Size = 3,000.
MingguI
PEMULIHAN N
Subset for alpha = 0.05
1 2
Tukey HSDa Formula F 3 24.00
Formula E 3 24.33
Formula D 3 29.00 29.00
Formula C 3 31.67 31.67
Formula B 3 39.67
Formula A 3 41.67
Sig. .506 .097
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguII
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula F 3 21.00
Formula E 3 22.00
Formula D 3 24.00 24.00
Formula C 3 25.00 25.00
Formula B 3 35.67 35.67
Formula A 3 41.67
Sig. .892 .082 .628
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguIII
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula F 3 16.00
Formula E 3 18.67 18.67
Formula D 3 19.33 19.33
Formula C 3 20.67 20.67
Formula B 3 30.33 30.33
Formula A 3 41.67
Sig. .859 .119 .135
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguIV
PEMULIHAN N
Subset for alpha = 0.05
1 2 3
Tukey HSDa Formula F 3 10.67
Formula E 3 14.67
Formula D 3 17.00 17.00
Formula C 3 18.33 18.33
Formula B 3 29.33 29.33
Formula A 3 41.67
Sig. .440 .080 .080
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
Kedalaman keriput
ANOVA
Sum of Squares df Mean Square F Sig.
Sebelum Between Groups 610.278 5 122.056 2.477 .092
Within Groups 591.333 12 49.278
Total 1201.611 17
MingguI Between Groups 171.111 5 34.222 1.046 .435
Within Groups 392.667 12 32.722
Total 563.778 17
MingguII Between Groups 91.111 5 18.222 .422 .825
Within Groups 518.667 12 43.222
Total 609.778 17
MingguIII Between Groups 248.944 5 49.789 1.624 .228
Within Groups 368.000 12 30.667
Total 616.944 17
MingguIV Between Groups 190.944 5 38.189 1.322 .319
Within Groups 346.667 12 28.889
Total 537.611 17
Sebelum
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 31.33
Formula E 3 38.33
Formula D 3 40.67
Formula C 3 44.33
Formula B 3 47.00
Lampiran
11.
(Lanjutan)
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguI
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 31.33
Formula E 3 34.33
Formula D 3 35.67
Formula C 3 36.00
Formula B 3 38.00
Formula A 3 41.33
Sig. .330
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguII
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 29.67
Formula E 3 31.33
Formula D 3 32.33
Formula C 3 34.00
Formula B 3 35.00
Formula A 3 36.33
Sig. .809
Means for groups in homogeneous subsets are displayed.
Lampiran 11.
(Lanjutan)
MingguIII
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 24.00
Formula E 3 29.67
Formula D 3 30.33
Formula C 3 33.00
Formula B 3 34.00
Formula A 3 35.33
Sig. .197
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
MingguIV
PEMULIHAN N
Subset for alpha
= 0.05
1
Tukey HSDa Formula F 3 19.67
Formula E 3 25.33
Formula D 3 26.33
Formula C 3 28.33
Formula B 3 29.00
Formula A 3 29.00
Sig. .336
Means for groups in homogeneous subsets are displayed.