The effects of rice seed dressing with Paenibacillus yonginensis Abstract 2016

FieldCropsResearch188(2016)121–132

ContentslistsavailableatScienceDirect

Field

Crops

Research

j o ur na l h o me p a g e :w w w . e l s e v i e r . c o m / l o c a t e / f c r

The

effects

of

rice

seed

dressing

with

Paenibacillus

yonginensis

and

silicon

on

crop

development

on

South

Korea’s

reclaimed

tidal

land

Eul-Su

Choi

a,1

,

Johan

Sukweenadhi

a,1

,

Yeon-Ju

Kim

a,∗

,

Ki

Hong

Jung

b

,

Sung-Cheol

Koh

c

,

Van-An

Hoang

a

_,

_Deok-Chun

_Yang

a,d,∗

a_{GraduateSchoolofBiotechnology,CollegeofLifeScience,KyungHeeUniversity,Yongin446-701,RepublicofKorea} b_{DepartmentofPlantMolecularSystemsBiotechnology&CropBiotechInstitute,KyungHeeUniversity,RepublicofKorea} c_{DepartmentofEnvironmentalEngineering,KoreaMaritimeUniversity,Busan,RepublicofKorea}

d_{DepartmentofOrientalMedicinalBiotechnology,CollegeofLifeScience,KyungHeeUniversity,Yongin446-701,RepublicofKorea}

a

r

t

i

c

l

e

i

n

f

o

Articlehistory:

Received27November2015

Receivedinrevisedform

29December2015

Accepted8January2016

Availableonline22January2016

Keywords:

PGPB

Seedimprovement

Fieldtest

Salinestress

GABA

a

b

s

t

r

a

c

t

Afieldtrialtoinvestigatetheeffectsofplantgrowthpromotingbacteria(PGPB)Paenibacillusyonginensis

(DCY84T_{)and/orSiO2seedcoatingonricegrowth(OryzasativaL.)wascarriedoutonreclaimedtidal} landinTaeanCountyinSouthKorea.ThefieldtestwasperformedtwicebetweenMay–October2014 andMay–October2015,inarandomizedcompleteblock(RCB)designwiththreereplications.Treatments consistedof:Mock,DCY84T_{-treatedseeds,SiO2-coatedseedsandDCY84}T_{-SiO2-treatedseedsandeach} treatmentareacoveredapproximately300m2_{.Duringtheearlydevelopmentalperiodofriceseedlings,} theSiO2coatingwithoutDCY84T_{ledtothemostfavorable30DASriceseedlingparameters.Moreover,} thecombinationofDCY84T_{andSiO2treatmentsresultedin2-foldgreaterfreshanddryweightsof60DAS} riceseedlingscomparedtoMockseedlings.DCY84T_{andSiO2,bothindividuallyandtogether,produced} agreatergrainyieldandagreatertotalyield;specifically,DCY84T_{andSiO2treatmentsyieldeda73%} and70%increaseinmasscomparedtoMockplants,respectively.RicetreatedwithbothDCY84T_and SiO2treatmentcontainedthehighestamountofAl,Fe,CaandMg,whichwere54%,169%,42%and67% higherthantheMockrice,respectively.Remarkably,DCY84T_{treatmenthadthemostphosphate[P],} potassium[K]andtotalnitrogen[T–N].DCY84T_{and/orSiO2treatmenthighlyincreasedthewholekernel} percentage.Thus,loweritsbrokenkernelpercentageto9.60–24.58%.Theproteincontentofthegrain withbothtreatmentswas7.2%,whichwasgreaterthanthatoftheMockgrain(6.0%).Thecontentof chlorophylla,bandcarotinoidinthericeleaveswhichweretreatedwithsilicaandDCY84hasincreased morethanthemockwithouttreatment.Afterharvest,theGABAcontentofbrownricewasincreased to1.9-fold(2014),1.5fold(2015)comparedtomockgrain,respectively.Overall,DCY84T_treatmentand SiO2coatingcanbeusefulmethodsforpromotinggrowthofriceunderconditionsofsalinestress.Results fromotherlaboratorytrialsandgreenhouseexperimentsarealsoprovided.

©2016PublishedbyElsevierB.V.

1. Introduction

The exponential growth of the human population demands

morefoodandthusenhancedcropproduction.Duringthe1960’s,

theGreenRevolutionwaslargelyresponsibleforincreasingfood

supply.Currently,twoalternativesexistforincreasingcereal

pro-∗ Correspondingauthorsat:DepartmentofOrientalMedicinalBiotechnology,

CollegeofLifeScience,KyungHeeUniversity,Yongin446-701,RepublicofKorea.

Fax:+82312022687.

E-mailaddresses:yeonjukim@khu.ac.kr(Y.-J.Kim),dcyang@khu.ac.kr

(D.-C.Yang).

1_{Theseauthorscontributedequallytothiswork.}

duction:(1)expandingfarmingintoareasthatarecurrentlynot

usedforfoodproductionor(2)attainingahigheryieldperunit

oflandareainexistingagriculturalareas(Linquistetal.,2011).

InSouthKorea,becausetheavailablefarmlandhadbeen

decreas-ingduetourbanizationandindustrialization,farmingonreclaimed

tidallandisoneofthecountry’seffortstoproducemorefood.

How-ever,agriculturalactivitiesonreclaimedtidallandaregenerally

difficultduetohighsalinityandlowamountsofnutrients(Cho

etal.,2008).A“salinesoil”isusuallydefinedassoilinwhichthe

electricalconductivity(EC)ofthesaturationextract(ECe)inthe

rootzoneismorethan4dSm−1 _at25◦_{C (equalto40mMNaCl)}

andtheexchangeablesodiumislessthan15%.Theyieldofmost

cropplantsisreducedatthisECe,thoughmanycropsexhibityield

http://dx.doi.org/10.1016/j.fcr.2016.01.005