vii
UJI AKTIVITAS EKSTRAK ETANOL DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) SEBAGAI ANTIKOLESTEROL MENGGUNAKAN
MENCIT JANTAN
ABSTRAK
Indonesia merupakan salah satu penghasil komoditas kelapa sawit Jacq.) terbesar di dunia. Daun kelapa sawit umumnya hanya sebagai limbah, namun semakin berkembangnya ilmu pengetahuan dan teknologi dapat digunakan untuk pengobatan berbagai macam penyakit, yaitu antibakteri, antioksidan, antihipertensi, antidiabetes, hepatoprotektor, toksisitas akut, obat penyembuh luka. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak etanol daun kelapa sawit (EEDKS) sebagai antikolesterol menggunakan mencit jantan.
Serbuk simplisia dimaserasi dengan pelarut etanol 80%, kemudian diuapkan menggunakan rotary evaporator (± 40oC) dengan bantuan vakum dan di freeze dryer (-40oC), selanjutnya ekstrak yang diperoleh diuji aktivitas antikolesterol terhadap mencit jantan yang dibagi menjadi 6 kelompok. Mencit diinduksi dengan pemberian lemak kambing dan kuning telur sebanyak 1% bb per oral selama 14 hari, kemudian diberikan selama 21 hari diberikan sediaan uji. Pengamatan dilakukan pada hari ke-7, 14 dan 21. Pada kelompok kontrol diberi Na-CMC 0,5%, kelompok uji EEDKS (dosis 100, 200, 300 dan 400 mg/kg bb), sebagai pembanding digunakan simvastatin dosis 1,3 mg/kg bb.
Hasil maserasi serbuk simplisia diperoleh ekstrak kental 108 g dan setelah di freeze dryer diperoleh sebanyak 98 g. EEDKS dosis 100, 200, 300 dan 400 mg/kg bb dapat menurunkan kadar kolesterol darah mencit selama 21 hari. Penurunan kadar kolesterol total yang memberikan hasil signifikan (p<0,05) pada EEDKS dosis 300 dan 400 mg/kg bb sama dengan simvastatin dosis 1,3 mg/kg bb. Gambaran histopatologi hati menunjukkan adanya penurunan degenerasi lemak pada sel hati mencit yang diberikan suspensi EEDKS dosis 300 dan 400 mg/kg bb, simvastatin dosis 1,3 mg/kg bb.
Kata kunci : daun kelapa sawit, kadar kolesterol total, histopatologi
viii
THE ACTIVITY OF ETHANOLIC EXTRACT OIL PALM LEAVES (Elaeis guineensis Jacq.) AS ANTI-CHOLESTEROL USING MALE MICE
ABSTRACT
Indonesia is one of the largest producer oil palm (Elaeis guineensis Jacq.) in the world. Palm leaves are generally only as waste, but the development of science and technology can be used for the treatment of some diseases, such as antibacterial, antioxidant, antihypertensive, antidiabetic, hepatoprotective, acute toxicity, wound healing drug. The purpose of this study was to determine the activity of the ethanol extract of palm leaves (EOPLE) as anti-cholesterol using male mice.
Simplicia of palm leaves was macerated with 80% ethanol and then evaporated using a rotary evaporator (± 40° C) with the aid of a vacuum and freeze dryer (-40° C). Simplicia and EOPLE as characterization and was tested against the anti-cholesterol activity male mice were divided into 6 groups. Mice induced by administration of goat fat and egg yolk as much as 1% bb orally for 14 days, then given the test preparation for 21 days. Observations were made on days 7, 14 and 21. In the control group were given CMC-Na 0.5%, the test group EEDKS (doses of 100, 200, 300 and 400 mg/kg bw), as a comparison used simvastatin doses of 1.3 mg/kg bw.
The result maceration of simplicia as thick extract 108 g and after freeze dryer 98 g. EOPLE at dose 100, 200, 300 and 400 mg / kg bw can decrease cholesterol total in mice for 21 days. Decrease in total cholesterol levels gave significant result (p<0.05) on EOPLE dose 300 and 400 mg / kg bw were same to simvastatin dose of 1.3 mg/kg bw (p<0.05). Histopathologic features showed a decrease liver fatty degeneration of the liver cells of mice were given a suspension EEDKS dose of 300 and 400 mg/kg bw and the simvastatin dose of 1.3 mg/kg bw.
Keywords : palm leaves, total cholesterol levels, histopathology
