Ekspresi Cluster of Differentiation 80 (CD80) dan Cluster of Differentiation 86 (CD86) dengan Cluster of Differentiation 8+ (CD8+) Pada Karsinoma Nasofaring Tipe Undifferentiated.

(1)

i

EKSPRESI

CLUSTER OF DIFFERENTIATION

_{80 (CD80) DAN}

CLUSTER OF DIFFERENTIATION

_{86 (CD86) DENGAN}

CLUSTER OF DIFFERENTIATION

₈

+

_(CD8

+

₎

PADA KARSINOMA NASOFARING

TIPE

UNDIFFERENTIATED

TESIS

Untuk memenuhi sebagian persyaratan dalam memperoleh keahlian dalam bidang Ilmu Kesehatan Telinga Hidung Tenggorok Bedah Kepala Leher

Oleh :

Nanang Suhana NIM. S921007004

PROGRAM PENDIDIKAN DOKTER SPESIALIS-I

I.K. TELINGA HIDUNG TENGGOROK BEDAH KEPALA LEHER FAKULTAS KEDOKTERAN UNIVERSITAS SEBELAS MARET

RSUD DR MOEWARDI SURAKARTA

(2)

ii

EKSPRESI CLUSTER OF DIFFERENTIATION _{80 (CD80)}_DAN CLUSTER OF DIFFERENTIATION ₈₆_{(CD86) DENGAN}

CLUSTER OF DIFFERENTIATION ₈+ _(CD8+_{) PADA}

KARSINOMA NASOFARING TIPE UNDIFFERENTIATED

Nanang Suhana, Made Setiamika, Hadi Sudrajad

Bagian Ilmu Kesehatan Telinga Hidung Tenggoro Bedah Kepala Leher Fakultas Kedokteran Universitas Sebelas Maret/RSUD Dr Moewardi

Surakarta

ABSTRAK

Latar Belakang : Karsinoma nasofaring (KNF) merupakan tumor ganas lapisan epitel nasofaring. Pada respon imun, ikatan molekul kostimulatori CD80, CD86 dan CD28 dikenal sebagai signal 2, yang mengakibatkan sel T teraktifasi.

Tujuan : Mengetahui hubungan ekspresi CD80 dan CD86 dengan CD8+ pada pasien Karsinoma Nasofaring Tipe Undifferentiated.

Metode : Desain penelitian ini adalah observasional analitik. Observasi penelitian dilakukan dengan pendekatan secara cross sectional. Penelitian dilakukan mulai bulan Oktober 2015 sampai besar sampel terpenuhi, setelah itu dilakukan pewarnaan imunohistokimia dan pembacaan ekspresi CD80, CD86 dan CD8+_.

Dilakukan penghitungan sel dengan penentuan score dengan Intensity Distribution Score (IDS) dan dianalisis dengan uji korelasi dan t test berpasangan.

Hasil : Berdasarkan perhitungan korelasi antara CD80 dengan CD8+ menghasilkan koefisien korelasi sebesar r = 0,342 (p = 0,032<0,05), dinyatakan korelasi signifikan. Hubungan CD86 dengan CD8+ menghasilkan koefisien korelasi sebesar r = 0,095. (p = 0,309>0,05) disimpulkan tidak terdapat korelasi. Perhitungan perbedaan ekspresi CD80 dengan CD86, p = 0,065 > 0,05 maka disimpulkan tidak terdapat perbedaan ekspresi.

Kesimpulan : Terdapat hubungan positif antara ekspresi CD80 dengan CD8+, tidak terdapat hubungan antara ekspresi CD86 dengan CD8+_{dan tidak terdapat}

perbedaan ekspresi antara CD80 dengan CD86 pada karsinoma nasofaring tipe Undifferentiated

(3)

iii

EXPRESSION OF CLUSTER OF DIFFERENTIATION 80 (CD80) AND CLUSTER OF DIFFERENTIATION 86 (CD86) WITH CLUSTER

OF DIFFERENTIATION 8+_(CD8+_{) ON NASOPHARYNGEAL}

CARCINOMA TYPE UNDIFFERENTIATED

Nanang Suhana, Made Setiamika, Hadi Sudrajad

Department of Otorhinolaryngology Head Neck Surgery Faculty of Medicine, University of Sebelas Maret / Dr Moewardi Hospital Surakarta

ABSTRACT

Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor of epithelial lining of the nasopharynx. On the immune response, bond costimulatory molecules CD80, CD86 and CD28 are known as signal 2, which results in activated T cells.

Objective: To determine the relationship of expression of CD80 and CD86 and CD8+ T cells in patients with nasopharyngeal carcinoma undifferentiated type.

Methods: The study design was observational analytic. Observations conducted research with cross sectional. The study was conducted from October 2015 through 30 samples, after it's done immunohistochemical staining and reading of the expression of CD80, CD86 and CD8+. Cell counting is done by determining the score with Intensity Score Distribution (IDS) and analyzed with correlation and paired t test.

Results: Based on the calculation of correlation between CD80 with CD8 + produces a correlation coefficient of r = 0.342 (p = 0.032 <0.05), revealed a significant correlation. The correlation between CD86 with CD8+ produces a correlation coefficient of r = 0.095. (p = 0.309> 0.05) concluded there is no correlation. Calculation differences with the expression of CD80+ and CD86+, p = 0.065> 0.05, it was concluded that there was no difference in expression.

Conclusion: There is a positive correlation between the expression of CD80 and CD8+, There was no relationship between the expression of CD86 and CD8+ and there is no difference between the expression of CD80 and CD86 on the type of undifferentiated nasopharyngeal carcinoma