characrerization of vector DNA microsatellite dengue hemorragic fever(DHF) aedes aegypti with enrichment method.

Characterization

of

vector

DNA

microsatellite

Dengue Hemorragic

Fever

{DHF)

Aedes

aeglpti with

Enricharent

Method

Hasmiwatil,

Diong

l{on'ljong2

and Dessy Arisanty 3

I

_Pa.asitology

Divisioru

_{Medical F'aculiy. Andalas Llniversily,}

Padang Emai I : hasmiwati6 5

@, gmail. co m

t

Biology

_{Departement, Mathemaics}

and Science Faculty, Andalas University, Padang

lBiochernestry Division,

Medical

lraculty,

AndrJas University, Padang

ABSTRACT

This

study aimed

to

characterizr

DNA

microsatellite of'

Aedes

aeg,tpti

with

enrichment

method,

DNA

was

extracted

and

then,

DNA

was

digested

with

restricted

enzyme

simultaneously

with

Alu I,

Rsal and

Hinc

II.

DNA

was adtrered

with MluI

adaptor (consist

of

21 - mer:

5'

CTC

ffc

CTT

ACG

CGT GGA

C'tA3'

and phospcrilated

with

25-mer in end

of

5': 3'ACA

CGA

GAA

CGA

A

TG GCA

CC'l'GAI'p

5').

Six

oligonucleotides have

(Allzo,

(CTbo

{CTho'(AC}20

(AG}zo and

(GC):o)

microsatellite

motifs,

and

hybrid by

using Hybon membrane. Result fragments

from hybridization

were eluled and

amplified

eCR)

with

21-mer pri21-mer. Result

of

study showed there were 700

bp,610

bp,

550

bp,490

bp dan 450 bp products

of

amplification

from

eluted

DNA.

'lhey

were

DNA

fragment candidatef which

conrained

motif

of

microsatellite. We concluded that enrichment method

could

result

DNA