B1J008164 13.

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LAMPIRAN

Lampiran 1. Tabel cara kerja Pengukuran Aktivitas Protease Digesti

PEREAKSI

VOLUME (Ml)

Standard

Sampel

Kontrol

Balanko

Tirosin

standard

0.50 -

-

-Kasein 5%

-

0.50 -

-Buffer

0.50

0.50 Ekstrak

enzim

-

0.25 -

-Akuades

0.25 -

0.75

0.75 TCA

-

-Di Inkubasi selama 60 menit pada suhu 37

0

c

TCA

0.75

0.75 -

0.75 Kasein 1%

-

0.50 -Di masukkan semua tabung kedalama refrigerator selama minimal 1 jam

Sentrifugasi pada kecepatan 6000 rpm selama 15 menit

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Lampiran 2. Tabel Cara Kerja Pengukuran Aktivitas Amilase Digesti

PEREAKSI

VOLUME (Ml)

Standard

Sampel

Kontrol

Balanko

Maltosa

standard

0.75 -

-

-Amilum 1%

-

0.75 -

-Ekstrak enzim

-

0.25 -

-Akuades

0.25 -

0.25

1.0 DNS

-

0.50 -Inkubasi selama 30 menit pada suhu 37

0

_c

DNS

0.50

0.50 -

0.50 Amilum 1%

-

0.75

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Lampiran 3. Tabel cara kerja Penentuan Kadar Protein Supernatan

PEREAKSI

VOLUME (Ml)

Standard

Sampel

Kontrol

Balanko

Kasein

standard

0.1 -

-

-Ekstrak enzim

-

0.1 -

-Pereaksi C

5.0 -

-

-Inkubasi selama 30 menit pada suhu 37

0

c

Pereaksi D

0.5

0.5 -

-Di kocok segera dan biarkan pada suhu kamar selama paling sedikit 10 menit

Ukur absorbansinyapada panjang gelombang 660 nm

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Lampiran 4. Analisis Aktivitas Protease pada Hepatopankreas dan Intestine

Univariate Analysis of Variance

Descriptive Statistics

Dependent Variable: Protease mmol/mg/menit (U/menit)

.58250 .188951 4

.78600 .039758 4

.68425 .166765 8

.77750 .078122 4

.93125 .073355 4

.85438 .108054 8

.60675 .090614 4

.84850 .293229 4

.72763 .238887 8

.65558 .147835 12

.85525 .170907 12

.75542 .186607 24

Tempat

Mean Std. Deviation N

Tests of Between-Subjects Effects

.372a 5 .074 3.123 .033

13.696 1 13.696 574.802 .000

.125 2 .063 2.624 .100

.239 1 .239 10.039 .005

.008 2 .004 .163 .850

.429 18 .024

14.497 24

.801 23

Source

Corrected Model Intercept Ukuran Tempat

Ukuran * Tempat Error

Total

Corrected Total

Type III Sum

of Squares df Mean Square F Sig.

R Squared = .465 (Adjusted R Squared = .316) a.

Post Hoc Tests

Estimates

.656 .045 .562 .749

.855 .045 .762 .949

Tempat H N

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Pairwise Comparisons

-.200* .063 .005 -.332 -.067

.200* .063 .005 .067 .332

(J) Tempat N

H (I) Tempat H N

Mean Difference

(I-J) Std. Error Sig.a Lower Bound Upper Bound 95% Confidence Interval for

Differencea

Based on estimated marginal means

The mean difference is significant at the .05 level. *.

Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments).

a.

Univariate Tests

.239 1 .239 10.039 .005

.429 18 .024

Contrast Error

Sum of

Squares df Mean Square F Sig.

The F tests the effect of Tempat. This test is based on the linearly independent pairwise comparisons among the estimated marginal means.

3. Ukuran * Tempat

.583 .077 .420 .745

.786 .077 .624 .948

.778 .077 .615 .940

.931 .077 .769 1.093

.607 .077 .445 .769

.848 .077 .686 1.011

Tempat

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Lampiran 5. Hasil Analisis Aktivitas Amilase pada Hepatopankreas dan

Intestine

Univariate Analysis of Variance

Between-Subjects Factors

B 8

Value Label N

Descriptive Statistics

Dependent Variable: Amilase mmol/mg/menit (U/menit)

.62225 .231998 4

1.41125 .097288 4

1.01675 .452754 8

.72125 .208410 4

1.14775 .174603 4

.93450 .289228 8

.72125 .226366 4

1.17325 .230295 4

.94725 .321034 8

.68825 .207065 12 1.24408 .201794 12 .96617 .347242 24 Tempat

Mean Std. Deviation N

Tests of Between-Subjects Effects

2.049a ₅ _.410 _10.181 _.000

22.403 1 22.403 556.640 .000

.031 2 .016 .390 .683

1.854 1 1.854 46.057 .000

.164 2 .082 2.034 .160

.724 18 .040

25.177 24

2.773 23

Source

Corrected Model Intercept Ukuran Tempat

Ukuran * Tempat Error

Total

Corrected Total

Type III Sum

of Squares df Mean Square F Sig.

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Post Hoc Tests

Estimates

.688 .058 .567 .810

1.244 .058 1.122 1.366

Tempat H N

Mean Std. Error Lower Bound Upper Bound 95% Confidence Interval

Pairwise Comparisons

-.556* .082 .000 -.728 -.384

.556* .082 .000 .384 .728

(J) Tempat N

H (I) Tempat H N

Mean Difference

(I-J) Std. Error Sig.a Lower Bound Upper Bound 95% Confidence Interval for

Differencea

Based on estimated marginal means

The mean difference is significant at the .05 level. *.

Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments).

a.

Univariate Tests

1.854 1 1.854 46.057 .000

.724 18 .040

Contrast Error

Sum of

Squares df Mean Square F Sig.

The F tests the effect of Tempat. This test is based on the linearly independent pairwise comparisons among the estimated marginal means.

3. Ukuran * Tempat

.622 .100 .412 .833

1.411 .100 1.201 1.622

.721 .100 .511 .932

1.148 .100 .937 1.358

.721 .100 .511 .932

1.173 .100 .963 1.384

Tempat

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Lampiran 6. Kurva Standar Larutan Tirosin

0 0,1 0,2 0,3 0,4 0,5 0,6

0 0,05 0,1 0,25 0,5 1,0

Konsentrasi (mg/ml)

A

b

sor

b

an

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Lampiran 7. Kurva Standar Larutan Maltosa

5

4

0

n

m

mg/ml

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Lampiran Gambar Penelitian

Gambar 1. Akuarium ikan uji

Gambar 2. Timbangan Mekanik

Gambar 3. Saluran digesti ikan nilem Gambar 4. Running Enzim

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