B1J008164 13.

LAMPIRAN

Lampiran 1. Tabel cara kerja Pengukuran Aktivitas Protease Digesti

PEREAKSI

VOLUME (Ml)

Standard

Sampel

Kontrol

Balanko

Tirosin

standard

0.50

-

-

-Kasein 5%

-

0.50

-

-Buffer

0.50

0.50

0.50

0.50

Ekstrak

enzim

-

0.25

-

-Akuades

0.25

-

0.75

0.75

TCA

-

-

-

-Di Inkubasi selama 60 menit pada suhu 37

0

c

TCA

0.75

0.75

-

0.75

Kasein 1%

-

-

0.50

-Di masukkan semua tabung kedalama refrigerator selama minimal 1 jam

Sentrifugasi pada kecepatan 6000 rpm selama 15 menit

Lampiran 2. Tabel Cara Kerja Pengukuran Aktivitas Amilase Digesti

PEREAKSI

VOLUME (Ml)

Standard

Sampel

Kontrol

Balanko

Maltosa

standard

0.75

-

-

-Amilum 1%

-

0.75

-

-Ekstrak enzim

-

0.25

-

-Akuades

0.25

-

0.25

1.0

DNS

-

-

0.50

-Inkubasi selama 30 menit pada suhu 37

0

_c

DNS

0.50

0.50

-

0.50

Amilum 1%

-

-

0.75

Lampiran 3. Tabel cara kerja Penentuan Kadar Protein Supernatan

PEREAKSI

VOLUME (Ml)

Standard

Sampel

Kontrol

Balanko

Kasein

standard

0.1

-

-

-Ekstrak enzim

-

0.1

-

-Pereaksi C

5.0

-

-

-Inkubasi selama 30 menit pada suhu 37

0

c

Pereaksi D

0.5

0.5

-

-Di kocok segera dan biarkan pada suhu kamar selama paling sedikit 10 menit

Ukur absorbansinyapada panjang gelombang 660 nm

Lampiran 4. Analisis Aktivitas Protease pada Hepatopankreas dan Intestine

Univariate Analysis of Variance

Descriptive Statistics

Dependent Variable: Protease mmol/mg/menit (U/menit)

.58250 .188951 4

.78600 .039758 4

.68425 .166765 8

.77750 .078122 4

.93125 .073355 4

.85438 .108054 8

.60675 .090614 4

.84850 .293229 4

.72763 .238887 8

.65558 .147835 12

.85525 .170907 12

.75542 .186607 24

Tempat

Mean Std. Deviation N

Tests of Between-Subjects Effects

Dependent Variable: Protease mmol/mg/menit (U/menit)

.372a 5 .074 3.123 .033

13.696 1 13.696 574.802 .000

.125 2 .063 2.624 .100

.239 1 .239 10.039 .005

.008 2 .004 .163 .850

.429 18 .024

14.497 24

.801 23

Source

Corrected Model Intercept Ukuran Tempat

Ukuran * Tempat Error

Total

Corrected Total

Type III Sum

of Squares df Mean Square F Sig.

R Squared = .465 (Adjusted R Squared = .316) a.

Post Hoc Tests

Estimates

Dependent Variable: Protease mmol/mg/menit (U/menit)

.656 .045 .562 .749

.855 .045 .762 .949

Tempat H N

Pairwise Comparisons

Dependent Variable: Protease mmol/mg/menit (U/menit)

-.200* .063 .005 -.332 -.067

.200* .063 .005 .067 .332

(J) Tempat N

H (I) Tempat H N

Mean Difference

(I-J) Std. Error Sig.a Lower Bound Upper Bound 95% Confidence Interval for

Differencea

Based on estimated marginal means

The mean difference is significant at the .05 level. *.

Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments).

a.

Univariate Tests

Dependent Variable: Protease mmol/mg/menit (U/menit)

.239 1 .239 10.039 .005

.429 18 .024

Contrast Error

Sum of

Squares df Mean Square F Sig.

The F tests the effect of Tempat. This test is based on the linearly independent pairwise comparisons among the estimated marginal means.

3. Ukuran * Tempat

Dependent Variable: Protease mmol/mg/menit (U/menit)

.583 .077 .420 .745

.786 .077 .624 .948

.778 .077 .615 .940

.931 .077 .769 1.093

.607 .077 .445 .769

.848 .077 .686 1.011

Tempat

Lampiran 5. Hasil Analisis Aktivitas Amilase pada Hepatopankreas dan

Intestine

Univariate Analysis of Variance

Between-Subjects Factors

B 8

Value Label N

Descriptive Statistics

Dependent Variable: Amilase mmol/mg/menit (U/menit)

.62225 .231998 4

1.41125 .097288 4

1.01675 .452754 8

.72125 .208410 4

1.14775 .174603 4

.93450 .289228 8

.72125 .226366 4

1.17325 .230295 4

.94725 .321034 8

.68825 .207065 12 1.24408 .201794 12 .96617 .347242 24 Tempat

Mean Std. Deviation N

Tests of Between-Subjects Effects

Dependent Variable: Amilase mmol/mg/menit (U/menit)

2.049a _{5 .410 10.181 .000}

22.403 1 22.403 556.640 .000

.031 2 .016 .390 .683

1.854 1 1.854 46.057 .000

.164 2 .082 2.034 .160

.724 18 .040

25.177 24

2.773 23

Source

Corrected Model Intercept Ukuran Tempat

Ukuran * Tempat Error

Total

Corrected Total

Type III Sum

of Squares df Mean Square F Sig.

Post Hoc Tests

Estimates

Dependent Variable: Amilase mmol/mg/menit (U/menit)

.688 .058 .567 .810

1.244 .058 1.122 1.366

Tempat H N

Mean Std. Error Lower Bound Upper Bound 95% Confidence Interval

Pairwise Comparisons

Dependent Variable: Amilase mmol/mg/menit (U/menit)

-.556* .082 .000 -.728 -.384

.556* .082 .000 .384 .728

(J) Tempat N

H (I) Tempat H N

Mean Difference

(I-J) Std. Error Sig.a Lower Bound Upper Bound 95% Confidence Interval for

Differencea

Based on estimated marginal means

The mean difference is significant at the .05 level. *.

Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments).

a.

Univariate Tests

Dependent Variable: Amilase mmol/mg/menit (U/menit)

1.854 1 1.854 46.057 .000

.724 18 .040

Contrast Error

Sum of

Squares df Mean Square F Sig.

The F tests the effect of Tempat. This test is based on the linearly independent pairwise comparisons among the estimated marginal means.

3. Ukuran * Tempat

Dependent Variable: Amilase mmol/mg/menit (U/menit)

.622 .100 .412 .833

1.411 .100 1.201 1.622

.721 .100 .511 .932

1.148 .100 .937 1.358

.721 .100 .511 .932

1.173 .100 .963 1.384

Tempat

Lampiran 6. Kurva Standar Larutan Tirosin

0 0,1 0,2 0,3 0,4 0,5 0,6

0 0,05 0,1 0,25 0,5 1,0

Konsentrasi (mg/ml)

A

b

sor

b

an

Lampiran 7. Kurva Standar Larutan Maltosa

5

4

0

n

m

mg/ml

Lampiran Gambar Penelitian

Gambar 1. Akuarium ikan uji

Gambar 2. Timbangan Mekanik

Gambar 3. Saluran digesti ikan nilem Gambar 4. Running Enzim

Gambar 7. Oven

Gambar 8. Timbangan Analitik