Isolasi Minyak Atsiri dari Sereh Merah (Andropogon citratus DC.) Segar dan Kering Serta Analisis Komponen Secara GC-MS

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1

2

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4 5 3

6

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C

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Lampiran 6. Perhitungan penetapan kadar air simplisia sereh merah

Kadar air = 100%

sampel Berat

air Volume



Kadar air I = 100% 5,98%v/b 013

, 008

, 010

,

No. Berat sampel (g) Volume awal (ml) Volume akhir (ml)

1. 5,013 0,9 1,2

2. 5,008 1,2 1,5

3. 5,010 1,5 1,8

(10)

Lampiran 7. Perhitungan penetapan kadar sari larut dalam air simplisia sereh merah

Kadar sari larut air = 100%

20 100 sampel

Berat

kosong cawan

berat sari

cawan Berat

  

Kadar sari I = 100% 10,967%b/b

20 100 015

, 5

090 , 45 45,200

 100 014

, 5

067 , 43 43,176

 100 012

, 5

122 , 45 45,226

 375 , 10 % 869 , 10 % 967 , 10

 



No. Berat sampel (g) Berat cawan kosong (g) Berat cawan sari (g)

1 5,015 45,090 45,200

2 5,014 43,067 43,176

3 5,012 45,122 45,226

(11)

Lampiran 8. Perhitungan penetapan kadar sari larut dalam etanol

Kadar sari larut etanol = 100%

20 100 sampel

Berat

kosong cawan

berat sari

cawan Berat

  

Kadar sari I = 100% 10,460%b/b

20 100 019

, 5

45,106 45,211

 100 006

, 5

46,333 46,432

 100 010

, 5

45,205 45,296

 081 , 9 % 888 , 9 % 460 , 10

 



No. Berat sampel (g) Berat cawan kosong (g) Berat cawan sari (g)

1 5,019 45,106 45,211

2 5,006 46,333 46,432

3 5,010 45,205 45,296

(12)

Lampiran 9. Perhitungan penetapan kadar abu total simplisia sereh merah

Kadar abu total = 100%

sampel Berat

abu Berat



Kadar abu total I = 100% 4,31%b/b 016

, 2 0,086

 

Kadar abu total II = 100% 4,19%b/b 025

, 2 0,085

 

Kadar abu total III = 100% 4,32%b/b 010

, 2

087 ,

No. Berat sampel (g) Berat abu (g)

1. 2,016 0,086

2. 2,025 0,085

3. 2,010 0,087

(13)

Lampiran 10. Perhitungan penetapan kadar abu tidak larut asam simplisia sereh merah

Kadar abu tidak larut asam = 100%

sampel Berat

abu Berat



Kadar abu I = 100% 0,44%b/b 003

, 2 0,009

 

Kadar abu II = 100% 0,39%b/b 005

, 2 0,008

 

Kadar abu III = 100% 0,34%b/b 008

, 2

006 ,

No. Berat sampel (g) Berat abu (g)

1. 2,003 0,009

2. 2,005 0,008

3. 2,008 0,006

(14)

Lampiran 11.Penetapan kadar minyak atsiri sereh merah

a. Kadar minyak atsiri sereh merah segar

Sampel I :

Berat sampel = 10,065 gram

Volume minyak atsiri = 0,04 ml

% Kadar minyak atsiri = x 100%

= ,

, x 100%

= 0,39% v/b

Sampel II

= ,

, x 100%

= 0,49% v/b

Sampel III

= ,

, x 100%

= 0,39% v/b

% Kadar minyak atsiri rata-rata= , % , % , %= 0,42% v/b

(15)

b. Kadar minyak atsiri sereh merah kering

Sampel I :

= ,

, x 100%

= 0,49% v/b

Sampel II

= ,

, x 100%

= 0,59% v/b

Sampel III

= ,

, x 100%

= 0,79% v/b

% Kadar minyak atsiri rata-rata = , % , % , %= 0,62% v/b

(16)

Lampiran 12.Penetapan indeks bias minyak atsiri sereh merah

a. Indeks bias minyak atsiri sereh merah segar

Sampel I = 1,456

Sampel II = 1,456

Sampel III =1,456

Indeks bias rata-rata = , , , = 1,456

b. Indeks bias minyak atsiri sereh merah kering

Sampel I = 1,456

Sampel II = 1,456

Sampel III = 1,456

Indeks bias rata-rata = , , , = 1,456

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15

14 CH2

18 26

CH3 H2O

CH2

14

C2H2

+

CH2

14

C4H4

52

CH2

14