٥
يﻮﻳر سرﺮﻳد ﺐﻴﺳآ ﺮﺑ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﻲﻧﺎﻣرد ﺮﺛا
شﻮﻣ رد لدﺮﺧ زﺎﮔ زا ﻲﺷﺎﻧ
ﻲﻣﺎﺴﻗ ﻪﻤﻃﺎﻓ -1
يرﺎﺼﻧا ﻼﻴﻟ -2
يداﺮﻣ ﻼﻬﺷ -3
مﺪﻘﻣ رﺎﺸﻓا ﻦﻴﺷﻮﻧ ﺮﺘﻛد -4
ﺮﺘﻛد ﻲﺘﺸﺧﺮﻴﻣ ﻦﻴﺷﻮﻧ -5
يﻮﻠﻋ ﻲﻠﻋ ﺪﻴﺳ ﺮﺘﻛد 6
هﺪﻴﻜﭼ
فﺪﻫ و ﻪﻨﻴﻣز رد:
نﺎﻴﻣ لدﺮﺧ زﺎﮔ ﻦﻴﻣوﺪﺼﻣ سرﺮﻳد ضراﻮﻋ
، يﻮﻳر تﻼﻜﺸﻣ زا
ﺮﺑ ﻪﻠﻤﺟ ﻮﻳر زوﺮﺒﻴﻓ و ﻦﻣﺰﻣ ﺖﻴﺸﻧو ي
،
نارﺎﻤﻴﺑ رد تﺪﻣﺪﻨﻠﺑ ﻲﻧاﻮﺗﺎﻧ ﺖﻠﻋ ﻦﻳﺮﺘﻤﻬﻣ ﻲﻣ
ﺪﺷﺎﺑ . ﻲﺑﺎﻬﺘﻟا ﺪﺿ تاﺮﺛا ياراد ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﺖﺳا
يﻮﻳر زوﺮﺒﻴﻓ رد و زا ﻲﺷﺎﻧ
ﻞﻠﻋ ﺮﻳﺎﺳ راد دﺮﺑرﺎﻛ د . ﺮﺑ و سﺎﺳا ﻦﻳا ﺶﻘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﺶﻨﺗ رد ﻮﻴﺗاﺪﻴﺴﻛا ﻮﻳر زوﺮﺒﻴﻓ و ﻦﻣﺰﻣ ﺖﻴﺸﻧوﺮﺑ ﺰﻧژﻮﺗﺎﭘ
ي
، ﻦﻳا رد
ﻪﻌﻟﺎﻄﻣ رﻮﻛﺬﻣ يوراد ﻲﻧﺎﻣرد ﺮﺛا سرﺮﻳد ضراﻮﻋ رد
لدﺮﺧ زﺎﮔ ﺶﻘﻧ و ﺶﻨﺗ رد ﻮﻴﺗاﺪﻴﺴﻛا ﺪﻧور
نآ ﺮﺛا ﺑ ﺪﺷ ﻲﺳرﺮ .
ﻖﻴﻘﺤﺗ شور ،ﻲﺑﺮﺠﺗ ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد:
ﻚﻳ هوﺮﮔ 6 ﺮﻧ يرﻮﺳ شﻮﻣ ﻲﻳﺎﺗ
، ﺎﺑ ﻪﻬﺟاﻮﻣ زا ﺲﭘ هﺎﻣ ﺞﻨﭘ لدﺮﺧ زﺎﮔ
ﺑ ﻪ ترﻮﺻ
ﻲﻗﺎﺸﻨﺘﺳا
، ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﺪﻧدﺮﻛ ﺖﻓﺎﻳرد
. ﻖﻴﻤﻋ ﻲﺷﻮﻬﻴﺑ لﺎﺒﻧد ﻪﺑ زا
شﻮﻣ ﺎﻫ ﻪﻧﻮﻤﻧ يﺮﻴﮔ و ﺲﭙﺳ ﻲﻳﺎﻴﻤﻴﺷﻮﻴﺑ يﺎﻫﺰﻴﻟﺎﻧآ
؛ﺪﺷ مﺎﺠﻧا ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ راﺪﻘﻣ
،
H2O2
ﻦﻴﻣﺎﺘﻳو و ﺪﺻرد ﻲﻗاﺮﺘﻓا شرﺎﻤﺷ ﻦﻴﻨﭽﻤﻫ و مﺮﺳC
لﻮﻠﺳ ﺎﻫ ي رد ﻲﺑﺎﻬﺘﻟا
ﻪﻳر ﺖﻓﺎﺑ نآ ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد و ،
ﺖﺒﺜﻣ ﺪﻫﺎﺷ ﺎﺑ درﻮﻣ هوﺮﮔ و ﻲﻔﻨﻣ ﺪﻫﺎﺷ ﺎﺑ ﺖﺒﺜﻣ ﺪﻫﺎﺷ هوﺮﮔ شور ﻪﺑ
t-student
و
Mann-Whitney
ﻪﺴﻳﺎﻘﻣ ﺪﺷ .
ﻪﺘﻓﺎﻳ ﺎﻫ نﺎﺸﻧ ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ: ﺶﻫﺎﻛ هﺪﻨﻫد
ﻲﻨﻌﻣ راد يﺎﻀﻓ ﺪﺻرد آ
ﻪﻳر ﻲﻟﻮﺋﻮﻟ
، ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا راﺪﻘﻣ ﺶﻫﺎﻛ و ﺎﻫ
H2O2
و
ﻦﻴﻣﺎﺘﻳو دﻮﺑ ﺖﺒﺜﻣ ﺪﻫﺎﺷ هوﺮﮔ ردC
. ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ راﺪﻘﻣ ﺶﻫﺎﻛ ﺚﻋﺎﺑ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﺰﻳﻮﺠﺗ
، حﺎﺸﺗرا ﺶﻫﺎﻛ
ﺖﻴﺳﻮﻔﻨﻟ و ﺎﻫ ﻲﻌﻴﺒﻃ دﺎﻘﻣ نﺪﺷ ﺮﻳ
H2O2
ﻦﻴﻣﺎﺘﻳو و ﺪﺷC
.
ﻪﺠﻴﺘﻧ ﺮﻴﮔ ي شﻮﻣ ﻪﻳر ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد ﺶﻫﺎﻛ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ: حﺮﻄﻣ ﻪﻴﺿﺮﻓ ود ﺖﺒﺜﻣ ﺪﻫﺎﺷ هوﺮﮔ رد ﺎﻫ
ﻲﻣ دﻮﺷ : ﻲﻜﻳ
حﺎﺸﺗرا يﺮﮕﻳد و يﻮﻳر زوﺮﺒﻴﻓ زوﺮﺑ و نژﻼﻛ بﻮﺳر لﻮﻠﺳ
ﺎﻫ يﺎﻬﺒﻴﺳآ ﺎﺑ ﻖﺑﺎﻄﻣ ﻊﻗاو رد ﻪﻛ ﻦﻣﺰﻣ بﺎﻬﺘﻟا زوﺮﺑ و ﻲﺑﺎﻬﺘﻟا ي
ﻣ رد لدﺮﺧ زﺎﮔ زا ﻲﺷﺎﻧ ﺖﺳا ﻲﻳﺎﻴﻤﻴﺷ نﺎﻣوﺪﺼ
. ﺶﻫﺎﻛ
H2O2
- ﻪﻛ ﻲﻣ زﺎﺗﻮﻤﺴﻳدﺪﻴﺴﻛاﺮﭘﻮﺳ ﻢﻳﺰﻧآ ﺶﻫﺎﻛ ﺖﻠﻋ ﻪﺑ ﺪﻧاﻮﺗ
ﺪﺷﺎﺑ - ﻦﻴﻣﺎﺘﻳو ﺶﻫﺎﻛ و نﺎﺸﻧC
ﻲﺘﻧآ عﺎﻓد ﺶﻫﺎﻛ هﺪﻨﻫد شﻮﻣ مﺮﺳ رد ﻲﻧاﺪﻴﺴﻛا
ﺖﺳا ﺎﻫ . ﺎﺑ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﺰﻳﻮﺠﺗ ﻲﻌﻴﺒﻃ
ﺶﻫﺎﻛ ﻲﺗرﺎﺒﻋ ﻪﺑ و هﺪﺷ ﺮﻛذ ﻞﻣﺎﻋ ود ندﺮﻛ ﺶﻨﺗ
ﻮﻴﺗاﺪﻴﺴﻛا
، ﭘ ﻲﺴﻛورﺪﻴﻫ ﺶﻫﺎﻛ ﺚﻋﺎﺑ ﻪﻳر ﻦﻴﻟوﺮ
) زوﺮﺒﻴﻓ ﺺﺧﺎﺷ (
ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا ﺶﻫﺎﻛو ﺎﻫ
) ﻲﺘﻓﺎﺑ ﻦﻣﺰﻣ بﺎﻬﺘﻟا ﺺﺧﺎﺷ (
ﻲﻣ دﻮﺷ .
هژاو ﺎﻫ يﺪﻴﻠﻛ ي لدﺮﺧ زﺎﮔ:
؛ ﺶﻨﺗ ﻮﻴﺗاﺪﻴﺴﻛا
؛ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ
؛ سرﺮﻳد ضراﻮﻋ
ﺪﻨﺟﺮﻴﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻤﻠﻋ ﻪﻠﺠﻣ )
هرود 13 هرﺎﻤﺷ ؛ 1 لﺎﺳ رﺎﻬﺑ ؛ 1385
(
ﺖﻓﺎﻳرد : 30 / 7 / 84 ﻲﻳﺎﻬﻧ حﻼﺻا
: 20 / 3 / 85 ﺬﭘ
شﺮﻳ : 23 / 3 / 85
نﺎﻬﻔﺻا ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ يﻮﺠﺸﻧاد1
قﺮﺷ نﺎﻤﻴﻜﺣ ﻲﺗﺎﻘﻴﻘﺤﺗ ﺖﻛﺮﺷ ﻮﻀﻋ و -
ﻘﺤﺗ و ﻲﻤﻠﻋ كﺮﻬﺷ نﺎﻬﻔﺻا ﻲﺗﺎﻘﻴ
ﻲﻜﺷﺰﭘ يﻮﺠﺸﻧاد2
ﻲﻤﻴﺷ سﺎﻨﺷرﺎﻛ3
ﻴﻜﺣ ﻲﺗﺎﻘﻴﻘﺤﺗ ﺖﻛﺮﺷ ؛ ﻤ
قﺮﺷ نﺎ - نﺎﻬﻔﺻا ﻲﺗﺎﻘﻴﻘﺤﺗ و ﻲﻤﻠﻋ كﺮﻬﺷ
ﺐﻴﺳآ ﻲﺷزﻮﻣآ هوﺮﮔ رﺎﻳدﺎﺘﺳا4
،ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ،ﻲﺳﺎﻨﺷ نﺎﻬﻔﺻا ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
قﺮﺷ نﺎﻤﻴﻜﺣ ﻲﺗﺎﻘﻴﻘﺤﺗ ﺖﻛﺮﺷ ﻮﻀﻋ ؛ﻚﺷﺰﭘ5
- و ﻲﻤﻠﻋ كﺮﻬﺷ ﻲﺗﺎﻘﻴﻘﺤﺗ
نﺎﻬﻔﺻا
لوﺆﺴﻣ هﺪﻨﺴﻳﻮﻧ6
ﻚﺷﺰﭘ ؛
سردآ : نﺎﻬﻔﺻا - ﺪﻴﺣﻮﺗ نﺎﺑﺎﻴﺧ -
شﺎﺒﻟﺰﻗ نﺎﺑﺎﻴﺧ -
هرﺎﻤﺷ 5 - نﺎﻤﻴﻜﺣ ﻲﺗﺎﻘﻴﻘﺤﺗ ﺖﻛﺮﺷ ،نﺎﻬﻔﺻا ﻲﺗﺎﻘﻴﻘﺤﺗ ،ﻲﻤﻠﻋ كﺮﻬﺷ قﺮﺷ
ﻦﻔﻠﺗ : 6698518 - 0311 ﺮﺑﺎﻤﻧ : 6698518 - 0311 ﻲﻜﻴﻧوﺮﺘﻜﻟا ﺖﺴﭘ :
٦ ﻪﻣﺪﻘﻣ لدﺮﺧ زﺎﮔ لﺎﺳ رد رﺎﺑ ﻦﻴﻟوا *
1822 ﻂﺳﻮﺗ Despretz
ﺎﻬﮕﻨﺟ رد و ﺪﺷ ﻪﺘﺧﺎﺳ ي
دﺪﻌﺘﻣ ي ﺑ ﻪ ﻲﻳﺎﻴﻤﻴﺷ حﻼﺳ ناﻮﻨﻋ
ﺖﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ .
قاﺮﻋ ﮓﻨﺟ رد نآ زا هدﺎﻔﺘﺳا ﻦﻳﺮﺧآ
ﺎﺑ ناﺮﻳا ﺎﻬﻟﺎﺳ رد ي 1983 ﺎﺗ 1988 ﺖﺳا هﺪﺷ شراﺰﮔ )
1 ( .
لدﺮﺧ زﺎﮔ و ﻚﻴﻠﻴﻓوﺮﺘﻜﻟا ﺐﻴﻛﺮﺗ ﻚﻳ
ا ﻜﻟ ﻴ ﻞ هﺪﻨﻨﻛ ﺖﺳا
؛
ﻦﻴﺌﺗوﺮﭘ ﻪﺑ ار دﻮﺧ ﻞﻴﻜﻟا هوﺮﮔ ﺎﻫﺪﻴﺳا و ﺎﻫ
ي ﻚﻴﺌﻠﻛﻮﻧ
لﻮﻠﺳ ﺎﻫ ي اﺰﺟا و هﺪﻧز ي
ﻞﻘﺘﻨﻣ نﺎﻤﻠﭙﻤﻛ ﻲﻣ
ﺪﻨﻛ دﺎﺠﻳا ﺐﺒﺳ و
لﻮﻜﻟﻮﻣ رد ﻊﻃﺎﻘﺘﻣ تﻻﺎﺼﺗا ﺎﻫ
ي فﺪﻫ ﻲﻣ دﻮﺷ ) 3،2 ( ﻪﺑ و
گﺮﻣ ﻪﺑ ﺮﺠﻨﻣ ﺖﻳﺎﻬﻧ رد ﺐﻴﺗﺮﺗ ﻦﻳا لﻮﻠﺳ
ﺎﻫ ﻲﻣ ددﺮﮔ ) 4 ( .
ﻤﺳ ﺪﻗ زا ﺮﺘﺸﻴﺑ رﺎﻴﺴﺑ لدﺮﺧ زﺎﮔ ﺖﻴ ﻦﺘﺸﻛ رد نآ تر
رد ﻦﻴﻣوﺪﺼﻣ ﻪﻠﺣﺮﻣ
دﺎﺣ ﺖﺳا ﻪﻬﺟاﻮﻣ .
سﺎﺳا ﺮﺑ ﻊﻗاو رد
مﺎﺠﻧا تﺎﻌﻟﺎﻄﻣ ور ﺮﺑ هﺪﺷ
ي رد لدﺮﺧ زﺎﮔ ﻲﻳﺎﻴﻤﻴﺷ ﻦﻴﻣوﺪﺼﻣ
قاﺮﻋ و ناﺮﻳا ﮓﻨﺟ و لوا ﻲﻧﺎﻬﺟ ﮓﻨﺟ
، 3 - 4
% زا رد داﺮﻓا
هدﺮﻛ تﻮﻓ ﻪﻬﺟاﻮﻣ نﺎﻣز ﺪﻧا
؛ ﻲﻟﺎﺣ رد ﻪﻛ
ﻣﺎﻤﺗ ﻲ ﻪﺑ ﺎﻬﻧآ
ﻢﺳ ﺎﺑ ﻪﻬﺟاﻮﻣ ضراﻮﻋ هﺪﺷ ﻼﺘﺒﻣ
ﺪﻧا . ﻧا ﻣاﺪ ضﺮﻌﻣ رد ﻪﻛ ﻲﻳﺎﻬ
ﻢﺳ ﺎﺑ سﺎﻤﺗ ﻢﺸﭼ ﻲﻨﻌﻳ ﺪﻨﺘﺴﻫ
ﺎﻫ
، ﺎﻬﻫار ي ﺖﺳﻮﭘ و ﻲﻳاﻮﻫ
ﻞﻤﺤﺘﻣ ار ﺐﻴﺳآ ﻦﻳﺮﺘﺸﻴﺑ ﻲﻣ
ﺪﻧﻮﺷ ) 1 (.
،ﺎﻀﻋا ﻦﻳا ﺲﭘ ﺎﻬﻟﺎﺳ
ﺎﺑ ﻪﻬﺟاﻮﻣ زا لدﺮﺧ زﺎﮔ
نﺎﻨﭽﻤﻫ ضراﻮﻋ رﺎﭼد
زا ﻲﺷﺎﻧ
ﻢﺳ
دﻮﺑ ﺪﻨﻫاﻮﺧ
؛ ﺑ ﻪ يرﻮﻃ ﻪﻌﻟﺎﻄﻣ رد ﻪﻛ Khateri
ﺮﺑ نارﺎﻜﻤﻫ و
ور ي 34000 ﻳا ﻲﻳﺎﻴﻤﻴﺷ حوﺮﺠﻣ ﻲﻧاﺮ
13 - 20 زا ﺲﭘ لﺎﺳ
ﻪﻬﺟاﻮﻣ
، نﺎﺣوﺮﺠﻣ سرﺮﻳد ﻪﺿرﺎﻋ ﻦﻳﺮﺘﻌﻳﺎﺷ ﺐﻴﺗﺮﺗ ﻪﺑ
رد
ﻪﻳر ﺎﻫ ) 5 / 42
% (
، ﻢﺸﭼ ﺎﻫ ) 39
% ( ﺖﺳﻮﭘ و )
5 / 24
% ( شراﺰﮔ
ﺖﺳا هﺪﺷ )
5 ( .
ﻮﻳر تﻼﻜﺸﻣ ي
ﺪﻣﺪﻨﻠﺑ ﻲﻧاﻮﺗﺎﻧ ﺖﻠﻋ ﻦﻳﺮﺘﻤﻬﻣ رد ت
لدﺮﺧ زﺎﮔ ﻲﻳﺎﻴﻤﻴﺷ نﺎﺣوﺮﺠﻣ ﺖﺳا
) 1 (.
ﺘﻣ دﻮﺟو ﻦﻳا ﺎﺑ ﺄ
ﻪﻧﺎﻔﺳ
ﭻﻴﻫ ﺮﺿﺎﺣ لﺎﺣ رد ﻪﻣﺎﻧﺮﺑ
اﺮﺑ ﻲﻧﺎﻣرد ي
ﺎﻬﺒﻴﺳآ ي ﻮﻳر ي ﻲﺷﺎﻧ
دراﺪﻧ دﻮﺟو لدﺮﺧ زﺎﮔ زا .
ﻮﺳ زا ي هﺪﺷ تﺎﺒﺛا ﺶﻘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺮﮕﻳد ﺶﻨﺗ
ﻮﻴﺗاﺪﻴﺴﻛا
ﻲﺘﻓﺎﺑ بﺎﻬﺘﻟا ﺰﻧژﻮﺗﺎﭘ رد )
6 (
، زا ﻲﺷﺎﻧ سرﺮﻳد ﺐﻴﺳآرد زﺎﮔ
رﺎﺠﻣ رد بﺎﻬﺘﻟا ﺪﻨﻳاﺮﻓ ندﻮﺑ راﺪﻳﺎﭘ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺰﻴﻧ لدﺮﺧ ي
*Sulfur Mustard
ﻲﻧﺎﺘﺤﺗ ﻲﺴﻔﻨﺗ )
7 ( رﺎﻤﻴﺑ ﺮﻴﺳ ندﻮﺑ هﺪﻧوﺮﺸﻴﭘ و ي
) 8 (
، لﺎﻤﺘﺣا
ندﻮﺑ ﻞﻴﺧد ﺶﻨﺗ
ﻪﻧﻮﮔ و ﻮﻴﺗاﺪﻴﺴﻛا ﺎﻫ
ي نﮋﻴﺴﻛا لﺎﻌﻓ و راد
ﻲﺘﻧآ دﻮﺒﻤﻛ ناﺪﻴﺴﻛا
ﺎﻫ ي حﺮﻄﻣ ﺎﻬﻧآ ﻞﺑﺎﻘﻣ رد نﺪﺑ ﻲﻋﺎﻓد
ﻲﻣ ﺪﺷﺎﺑ
؛ ﻧاﻮﺘﺑ ﻪﻛ ﻲﻳﺎﻫوراد ﻦﻳاﺮﺑﺎﻨﺑ ﻨ
تﺪﺷ زا ﺪ ﺶﻨﺗ
ﻮﻴﺗاﺪﻴﺴﻛا
ﺪﻨﻫﺎﻜﺑ
، ﺮﻴﮔﻮﻠﺟ رد ي
ضراﻮﻋ نﺎﻣرد ﺎﻳ و ﺐﻴﺳآ ﺖﻓﺮﺸﻴﭘ زا
سرﺮﻳد ﻮﻳر ي نﺎﺣوﺮﺠﻣ ﻲﻣ
ﻨﻧاﻮﺗ ﻣ ﺪ ﺆ ﺪﻧﻮﺷ ﻊﻗاو ﺮﺛ .
ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ وراد ﻚﻳ A
ي ﺎﺑ ﻪﻛ ﺖﺳا ﻚﻴﺴﻛﻮﺗﻮﺘﻴﺳ
ﺮﺑ ﻢﻴﻘﺘﺴﻣ ﺮﺛا لﻮﻠﺳ
ﺎﻫ ي ﺮﺑ ﻢﻴﻘﺘﺴﻣ ﺮﻴﻏ ﺮﺛا و T لﻮﻠﺳ
ﺎﻫ ي
،B بﺎﻬﺘﻟا ﺶﻫﺎﻛ و ﻲﻨﻤﻳا ﻢﺘﺴﻴﺳ ﻒﻴﻌﻀﺗ ﺚﻋﺎﺑ ﻲﻣ
دﻮﺷ ) 9 (
؛
ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ ﺞﻳﺎﺘﻧ سﺎﺳا ﺮﺑ نآ زا ﻪﺘﺷﺬﮔ
، ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ
دراﻮﻣ رد ي ﻮﻳر زوﺮﺒﻴﻓ نﺎﻣرد رد ي
ﻳﺪﻳا ﻣ ﻚﻴﺗﺎﭘﻮ ﺆ
ﺖﺳا هدﻮﺑ ﺮﺛ
؛
ﺑ ﻪ يرﻮﻃ ﻪﻌﻟﺎﻄﻣ سﺎﺳا ﺮﺑ ﻪﻛ Inase
نارﺎﻜﻤﻫ و
، زا
ﻪﻠﻌﺷ
ﺪﺠﻣ نﺪﺷرو د
(Re-exacerbation) ﻮﻳر زوﺮﺒﻴﻓ
ي
ﺮﻴﮔﻮﻠﺟ ﻚﻴﺗﺎﭘﻮﻳﺪﻳا ي
ﻲﻣ ﻨﻛ ﺪ اﺮﺑ ار رﺎﻤﻴﺑ ﺲﻧﺎﺷ و ي
ﺎﻘﺑ ي
ﺶﻳاﺰﻓا ﻲﻧﻻﻮﻃ ﻲﻣ
ﺪﻫد ) 10 ( .
ﺮﺛا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ
نﺎﻣرد و بﺎﻬﺘﻟا ﺶﻫﺎﻛ ﺮﺑ
ﻮﻳر زوﺮﺒﻴﻓ ي
ﻦﻳا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ و زوﺮﺒﻴﻓ و ﻦﻣﺰﻣ ﺖﻴﺸﻧوﺮﺑ ﻪﻛ
ﻮﻳر ي ود ﺖﻣﻼﻋ ﻮﻳر سرﺮﻳد ﺐﻴﺳآ رد ﻊﻳﺎﺷ ﻲﻨﻴﻟﺎﺑ ي
زا ﻲﺷﺎﻧ
لدﺮﺧ زﺎﮔ ﻲﻟﺎﻤﺘﺣا ﺶﻘﻧ ﻪﺑ ﺖﻳﺎﻨﻋ ﺎﺑ و ﺪﻨﺘﺴﻫ
ﺶﻨﺗ ﻮﻴﺗاﺪﻴﺴﻛا
سرﺮﻳد ضراﻮﻋ دﺎﺠﻳا رد لدﺮﺧ زﺎﮔ
، فﺪﻫ ﺎﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ
ﻦﻴﻴﻌﺗ ﻲﻧﺎﻣرد ﺮﺛا وراد
ي ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ سرﺮﻳد ضراﻮﻋ ﺮﺑ
ﻮﻳر ي لدﺮﺧ زﺎﮔ ﺶﻘﻧ و
ﺶﻨﺗ رد ﻮﻴﺗاﺪﻴﺴﻛا ﺪﻧور
ﺮﻴﺛﺄﺗ نآ و
ﺰﻧژﻮﺗﺎﭘ زا ﻲﺷﺎﻧ سرﺮﻳد ﺐﻴﺳآ لدﺮﺧ زﺎﮔ
ﺪﺷ مﺎﺠﻧا .
ﻖﻴﻘﺤﺗ شور
رد ﻦﻳا ﻪﻌﻟﺎﻄﻣ ﺑﺮﺠﺗ ﻲ
، 20 ﺮﻧ يرﻮﺳ شﻮﻣ نزو ﺎﺑ
2 23±
مﺮﮔ ﻪﻛ ﺎﻬﻧآ زا ﺎﻣد رد ي لﺮﺘﻨﻛ و هﺪﺷ ﻪﺧﺮﭼ ﺎﻨﺷور ﻲﻳ - رﺎﺗ ﻳ ﻜ ﻲ
12 ﺬﻐﺗ و ﻪﺘﻋﺎﺳ ﻳ
ﻪ اﺬﻏ ﺎﺑ ي يراﺪﻬﮕﻧ دراﺪﻧﺎﺘﺳا ﻲﻣ
ﺷ ﺪ
، ﻪﺑ
تﺪﻣ 15 ﻪﻘﻴﻗد ضﺮﻌﻣ رد mg/m3
600 لدﺮﺧ زﺎﮔ ﺑ
ﻪ ترﻮﺻ
ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻲﺴﻔﻨﺗ .
هﺎﻣ ﺞﻨﭘ زا ﺲﭘ
، زا 12 هﺪﻧﺎﻣ هﺪﻧز شﻮﻣ
،
هوﺮﮔ ﻚﻳ 6
ﺎﺗ ﻳ و ﺖﺒﺜﻣ ﺪﻫﺎﺷ ناﻮﻨﻋ ﻪﺑ ﻲ ﻚﻳ
هوﺮﮔ 6 ﺎﺗ ﻳ ﻲ
ﺑ ﻪ ناﻮﻨﻋ درﻮﻣ و 8 ﻮﻣ ش رﻮﺳ ي زﺎﮔ ﺎﺑ ﻪﻬﺟاﻮﻣ نوﺪﺑ ﻢﻟﺎﺳ ﺮﻧ
٧
لدﺮﺧ ﺑ ﻪ ﺪﻧﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﻲﻔﻨﻣ ﺪﻫﺎﺷ ناﻮﻨﻋ .
هوﺮﮔ رد
درﻮﻣ
، زود ﺎﺑ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ لوا زور ﻪﺳ رد µg/kg/day
20 و
يﺪﻌﺑ زور ﻪﺳ رد ﺎﺑ
ﻒﺼﻧ زود ﺑ
ﻪ ﻲﻗﺎﻔﺻ ﻞﺧاد ﻖﻳرﺰﺗ ترﻮﺻ
ﺰﻳﻮﺠﺗ ﺪﻳدﺮﮔ . زا ﺲﭘ وراد ﻊﻄﻗ زا ﻪﺘﻔﻫ ﻚﻳ ﺖﺷﺬﮔ
، زا
شﻮﻣ ﺎﻫ
،ﻖﻴﻤﻋ ﻲﺷﻮﻬﻴﺑ ﺖﺤﺗ ﻪﻧﻮﻤﻧ
نﻮﺧ ﺪﺷ ﻪﺘﻓﺮﮔ .
ﻪﻳر
ياﺮﺑ ﭗﭼ ﻪﻳر و ﻲﭘﻮﻜﺳوﺮﻜﻴﻣ ﻊﻃﺎﻘﻣ ﻪﻴﻬﺗ ياﺮﺑ ﺖﺳار ﻲﺳرﺮﺑ
ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ياﻮﺘﺤﻣ ﺪﺷ ﻪﺘﺷادﺮﺑ
. شﻮﻣ ﭗﭼ ﻪﻳر ﺎﻫ
اﺮﺑ ي ﺑ نﺪﺷ ﻚﺸﺧ ﻪ
تﺪﻣ 6 ﺖﻋﺎﺳ ﺎﻣد رد
°c ي 90 راﺮﻗ
هداد ﺪﺷ و ﺲﭙﺳ ﻦﻳزﻮﺗ ﻳدﺮﮔ ﺪ . اﺮﺑ ي نژﻼﻛ ﺰﻴﻟورﺪﻴﻫ
، ﻪﻳر ﺎﻫ
ﻚﻳرﺪﻳﺮﻠﻛ ﺪﻴﺳا رد 6
ﺎﻣد و لﺎﻣﺮﻧ
°c ي 110 ﺑ ﻪ تﺪﻣ 12
ﺪﺷ هداد راﺮﻗ ﺖﻋﺎﺳ ﺪﻧ
شور ﻪﺑ و Woessner
راﺪﻘﻣ
ﻛورﺪﻴﻫ ﺪﻳدﺮﮔ ﻦﻴﻴﻌﺗ ﺎﻬﻧآ ﻦﻴﻟوﺮﭘ ﻲﺴ )
11 ( . مﺎﺠﻧا زا ﺲﭘ
هدﺎﻣآ ﻞﺣاﺮﻣ زﺎﺳ
ي شﻮﻣ ﻪﻳر زا شﺮﺑ ﻪﻴﻬﺗ و ﺖﻓﺎﺑ و ﺎﻫ
ﮓﻧر يﺰﻴﻣآ H&E
، ﻪﻣﺎﻧﺮﺑ زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﻧﺎﻳار
يا Matlab
،
ﻦﻴﻴﻌﺗ ﺎﻬﻧآ ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد ﺪﺷ
. مﻻ نآ ﺮﺑ هوﻼﻋ ﺎﻫ
ي
ﻪﻴﻬﺗ هﺪﺷ
، ﻂﺳﻮﺗ ﺐﻴﺳآ ﺺﺼﺨﺘﻣ ﻲﺳﺎﻨﺷ
شرﺎﻤﺷ و ﻲﺳرﺮﺑ
ﻲﻗاﺮﺘﻓا ﺳ
لﻮﻠ ﺎﻫ ي ﻪﻳر ﺖﻓﺎﺑ رد ﻲﺑﺎﻬﺘﻟا مﺎﺠﻧا
ﺷ ﺪ . رد
ﻪﻧﻮﻤﻧ ﺎﻫ ي شﻮﻣ مﺮﺳ
،ﺎﻫ هزاﺪﻧا ﺮﻴﮔ ي H2O2
شور ﻪﺑ Wolff
) 12 ( و ﻦﻴﻣﺎﺘﻳو شور ﻪﺑ C
و Roe Kuether )
13 ( مﺎﺠﻧا
ﻳدﺮﮔ ﺪ . هداد يوﺮﻴﭘ لﺎﻣﺮﻧ ﻊﻳزﻮﺗ زا ﺎﻫ
رﺎﻣآ نﻮﻣزآ زا هدﺎﻔﺘﺳا ﺎﺑ ي
فوﺮﮔﻮﻤﻟﻮﻛ -
ﻤﺳا ﻴ فﻮﻧﺮ
، ﺳرﺮﺑ ﻲ ﺪﺷ . ا ﺮﺑ ﻳ سﺎﺳا ﻦ اﺮﺑ
ي
ﺎﻘﻣ ﻪﺴﻳ ﻐﺘﻣ ﻴ ﺎﻫﺮ ﻲﻳ زﻮﺗ زا ﻪﻛ ﻳ
ﭘ لﺎﻣﺮﻧ ﻊ ﻴ
وﺮ ي ﻲﻣ ﺪﻧدﺮﻛ
، زا
رﺎﻣآ نﻮﻣزآ ي
t-student اﺮﺑ و
ي ﺎﺳ ﻳ نﻮﻣزآ زا دراﻮﻣ ﺮ
ﺮﻴﻏ ﺮﺘﻣارﺎﭘ ي Mann-Whitney ﻲﻨﻌﻣ ﺢﻄﺳ رد
يراد
05 / P≤0 ﺪﺷ هدﺎﻔﺘﺳا .
ﻪﺘﻓﺎﻳ ﺎﻫ ﺎﺘﻧ سﺎﺳا ﺮﺑ ﻳ
فوﺮﮔﻮﻤﻟﻮﻛ نﻮﻣزآ زا ﻞﺻﺎﺣ ﺞ -
ﻤﺳا ﻴ فﻮﻧﺮ
،
اﺮﺑ ي لﺎﻣﺮﻧ دروآﺮﺑ زﻮﺗ ندﻮﺑ
ﻳ هداد ﻊ ﺎﻫ
، ﻫ ﺎﻬﻨﺗ ﻴ ﺴﻛورﺪ ﻲ ﻟوﺮﭘ ﻴ ﻦ
زﻮﺗ زا ﻳ ﭘ لﺎﻣﺮﻧ ﻊ ﻴ
وﺮ ي ﻤﻧ ﻲ دﺮﻛ .
لﻮﻠﺳ حﺎﺸﺗرا و ﺶﻫﺎﻛ ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد يﺎﻫ
ﻲﺘﻴﺳﻮﻔﻨﻟ رد
ﺖﺒﺜﻣ ﺪﻫﺎﺷ هوﺮﮔ )
ﻞﻜﺷ 1 ( ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد
ﻪﻧﻮﻤﻧ يﺎﻫ ﻢﻟﺎﺳ ) ﻞﻜﺷ 2 (
، دﻮﺑ ﻪﺘﻓﺎﻳ ﺶﻳاﺰﻓا .
راﺪﻘﻣ
ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ﺶﻳاﺰﻓا ﻪﻳر ﺖﻓﺎﺑ
ﻳ دﻮﺑ ﻪﺘﻓﺎ
؛ ﻦﻴﻨﭽﻤﻫ
ﺮﻳدﺎﻘﻣ H2O2 و و ﻦﻴﻣﺎﺘﻳ شﻮﻣ مﺮﺳ رد C
ﺎﻫ ي ﺖﺒﺜﻣ ﺪﻫﺎﺷ
ﻪﻧﻮﻤﻧ زا ﺮﺘﻤﻛ يﺎﻫ
ﻢﻟﺎﺳ دﻮﺑ .
ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ﺮﻳدﺎﻘﻣ رد
هوﺮﮔ درﻮﻣ
، هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ
ﻪﺘﻓﺎﻳ ﺶﻫﺎﻛ ﺖﺒﺜﻣ ﺪﻫﺎﺷ دﻮﺑ
) ﻞﻜﺷ 3 (
؛ ﻦﻴﻨﭽﻤﻫ ﺪﺻرد
ﺎﻀﻓ ي ﻲﻟﻮﺋﻮﻟآ ﺶﻳاﺰﻓا ﺳﻮﻔﻨﻟ حﺎﺸﺗرا و ﻴ
ﺖ دﻮﺑ ﻪﺘﻓﺎﻳ ﺶﻫﺎﻛ ﺎﻫ
؛
ﻦﻳا ﺗوﺎﻔﺗ ﺎﻬ رﺎﻣآ ﺮﻈﻧ زا ي
ﻨﻌﻣ ﻲ راد دﻮﺒﻧ ﻤﻫ ؛ ﻦﻴﻨﭽ ﺮﻳدﺎﻘﻣ
H2O2
ﻦﻴﻣﺎﺘﻳو و ﻪﺘﻓﺎﻳ ﺶﻳاﺰﻓا ﺖﺒﺜﻣ ﺪﻫﺎﺷ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧC
دﻮﺑ
؛ ﻪﺘﺒﻟا
ﺪﺣ ﻪﺑ ي ﻨﻌﻣ توﺎﻔﺗ ﻲﻔﻨﻣ ﺪﻫﺎﺷ هوﺮﮔ ﺎﺑ ﻪﻛ ﻲ
راد ي ار
ﺪﻧ نﺎﺸﻧ اد .
ﻞﻜﺷ 1 - ﺎﻤﻧ ى ﺖﺒﺜﻣ ﺪﻫﺎﺷ ﻪﻳر ﻲﺘﻓﺎﺑ
ﻞﻜﺷ 2 - ﺎﻤﻧ ى ﻲﻔﻨﻣ ﺪﻫﺎﺷ ﻪﻳر ﻲﺘﻓﺎﺑ
٨
ﻞﻜﺷ 3 - ﺎﻤﻧ ى شﻮﻣ ﻪﻳر ﻲﺘﻓﺎﺑ ﻪﺘﻓﺮﮔ وراد
ﺮﻳدﺎﻘﻣ رﺎﻴﻌﻣ فاﺮﺤﻧا و ﻦﻴﮕﻧﺎﻴﻣ ﺎﻀﻓ ﺪﺻرد
ي ﻲﻟﻮﺋﻮﻟآ
،
ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ يازا ﻪﺑ
ﻪﻳر نزو ﺪﺣاو
، ﺪﺻرد حﺎﺸﺗرا
ﺖﻴﺳﻮﻔﻨﻟ ﺎﻫ ﺮﻳﺎﺳ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد لﻮﻠﺳ
ﺎﻫ ي ﻲﺑﺎﻬﺘﻟا
، ﺖﻈﻠﻏ
ﻲﻣﺮﺳ H2O2 ﻦﻴﻣﺎﺘﻳو و ﺎﻬﻫوﺮﮔ رد C
ي ﻒﻠﺘﺨﻣ لوﺪﺟ رد 1
،
ﻪﺋارا ﺷ ﺖﺳا هﺪ .
ﻲﻨﻌﻣ ﺢﻄﺳ يراد
ﻓ ﺪﺻرد يﺎﻬﺼﺧﺎﺷ ،ﻲﻟﻮﺋﻮﻟآ يﺎﻀ
حﺎﺸﺗرا ﺪﺻرد ،ﻪﻳر نزو ﺪﺣاو يازا ﻪﺑ ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ﺖﻴﺳﻮﻔﻨﻟ لﻮﻠﺳ ﺮﻳﺎﺳ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد ﺎﻫ ﺖﻈﻠﻏ ،ﻲﺑﺎﻬﺘﻟا يﺎﻫ
ﻲﻣﺮﺳ H2O2
ﻦﻴﻣﺎﺘﻳو و لوﺪﺟ رد ﻒﻠﺘﺨﻣ يﺎﻬﻫوﺮﮔ ﻦﻴﺑ C
2 ﻪﺋارا ﺖﺳا هﺪﺷ .
لوﺪﺟ 1 - يازا ﻪﺑ ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ،ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد رﺎﻴﻌﻣ فاﺮﺤﻧا و ﻦﻴﮕﻧﺎﻴﻣ ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا ﺪﺻرد ،ﻪﻳر نزو ﺪﺣاو
ﺎﻫ
لﻮﻠﺳ ﺮﻳﺎﺳ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد ﺖﻈﻠﻏ ،ﻲﺑﺎﻬﺘﻟا يﺎﻫ
ﻲﻣﺮﺳ H2O2 ﻦﻴﻣﺎﺘﻳو و ﻒﻠﺘﺨﻣ يﺎﻬﻫوﺮﮔ ردC
هوﺮﮔ
ﺺﺧﺎﺷ ﻲﻔﻨﻣ ﺪﻫﺎﺷ
ﺖﺒﺜﻣ ﺪﻫﺎﺷ
درﻮﻣ
ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد
74 / 1 40±
/ 60 00
/ 3 96±
/ 56 84
/ 3 91±
/ 58
ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ
(mg/g)
42
/ 0 68±
/ 6 08
/ 1 50±
/ 7 19
/ 1 5±
ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا ﺪﺻرد لﻮﻠﺳ ﺮﻳﺎﺳ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد ﺎﻫ
ﻲﺑﺎﻬﺘﻟا يﺎﻫ
17 / 2 8±
/ 4 7
/ 5 12±
4
/ 3 2±
/ 5
H2O2 (µm)
88 / 3 82±
/ 12 08
/ 3 01±
/ 8 85
/ 1 2±
/ 17
ﻦﻴﻣﺎﺘﻳو )C mg/dL
( 37
/ 17 25±
/ 34 95 / 2 84±
/ 16 40
/ 3 16±
/ 41
لوﺪﺟ 2 - ﻲﻨﻌﻣ ﺢﻄﺳ و يازا ﻪﺑ ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ،ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد يﺎﻬﺼﺧﺎﺷ راد
ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا ﺪﺻرد ،ﻪﻳر نزو ﺪﺣا ﺎﻫ
لﻮﻠﺳ ﺮﻳﺎﺳ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد ﻲﻣﺮﺳ ﺖﻈﻠﻏ ،ﻲﺑﺎﻬﺘﻟا يﺎﻫ
H2O2
ﻦﻴﻣﺎﺘﻳو و ﻒﻠﺘﺨﻣ يﺎﻬﻫوﺮﮔ ﻦﻴﺑC
هوﺮﮔ
ﺺﺧﺎﺷ ﻲﻨﻌﻣ ﺢﻄﺳ
يراد
فوﺮﮔﻮﻤﻟﻮﻛ -
فﻮﻧﺮﻴﻤﺳا ﺖﺒﺜﻣ و ﻲﻔﻨﻣ ﺪﻫﺎﺷ
درﻮﻣ و ﺖﺒﺜﻣ ﺪﻫﺎﺷ
ﻲﻟﻮﺋﻮﻟآ يﺎﻀﻓ ﺪﺻرد
265 / 0 026
/ 0 -
ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ
(mg/g)
023
/ 0 01
/ 0 05
/ 0
ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا ﺪﺻرد لﻮﻠﺳ ﺮﻳﺎﺳ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد ﺎﻫ
ﻲﺑﺎﻬﺘﻟا يﺎﻫ -
043
/ 0 -
H2O2 (µm)
778 / 0 000
/ 0 000
/ 0
ﻦﻴﻣﺎﺘﻳو
C (mg/dL)
064
/ 0 000
/ 0 001
/ 0
ﺚﺤﺑ تﺪﻣﺪﻨﻠﺑ ﻲﻧاﻮﺗﺎﻧ ﺖﻠﻋ ﻦﻳﺮﺘﻤﻬﻣ يﻮﻳر تﻼﻜﺸﻣ
ﺖﺳا ﻲﻳﺎﻴﻤﻴﺷ نﺎﺣوﺮﺠﻣ )
1 .(
يور ﺮﺑ ﻪﻌﻟﺎﻄﻣ ﻚﻳ ﺞﻳﺎﺘﻧ 197
ﺳ ﻪﻛ داد نﺎﺸﻧ ﻲﻧاﺮﻳا زﺎﺑﺮ 10
ﻪﻬﺟاﻮﻣ ﻚﻳ زا ﺲﭘ لﺎﺳ
ﻦﻣﺰﻣ ﺖﻴﺸﻧوﺮﺑ ﻞﻣﺎﺷ يﻮﻳر ضراﻮﻋ ،لدﺮﺧ زﺎﮔ ﺎﺑ ﻦﻴﮕﻨﺳ ) 58 (%
يﻮﻳر زوﺮﺒﻴﻓ ، )
12 (%
ﻢﺳآ ، ) 10 (%
ﻚﻳرﺎﺑ ، يﺎﻬﻫار نﺪﺷ گرﺰﺑ ﻲﻳاﻮﻫ
) 9 (%
يزﺎﺘﻜﺸﻧوﺮﺑ و )
8 (%
ﺖﺳا هدﻮﺑ )
14 .(
ﺞﻳﺎﺘﻧ
نﺎﺸﻧ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﻲﻨﻌﻣ ﺶﻫﺎﻛ هﺪﻨﻫد
يﺎﻀﻓ ﺪﺻرد راد
ﻮﺋﻮﻟآ ﺖﺒﺜﻣ ﺪﻫﺎﺷ هوﺮﮔ رد ﻲﻟ )
ﺖﻓﺎﻳرد لدﺮﺧ زﺎﮔ هﺪﻨﻨﻛ (
ﺖﺒﺴﻧ
ﻲﻣ ﻲﻔﻨﻣ ﺪﻫﺎﺷ هوﺮﮔ ﻪﺑ ﻲﻣ ﻪﻛ ﺪﺷﺎﺑ
؛ﺪﺷﺎﺑ ﺖﻠﻋ ود ﻪﺑ ﺪﻧاﻮﺗ
لﻮﻠﺳ حﺎﺸﺗرا لوا و ﻲﺘﻓﺎﺑ نژﻼﻛ ﺶﻳاﺰﻓا مود و ﻲﺑﺎﻬﺘﻟا يﺎﻫ
زوﺮﺒﻴﻓ دﺎﺠﻳا .
ﻪﻧﻮﻤﻧ رد ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد يژﻮﻟﻮﺗﺎﭘ يﺎﻫ
٩
ﻲﺳرﺮﺑ ﺖﻴﺳﻮﻔﻨﻟ حﺎﺸﺗرا ،هﺪﺷ دﻮﺑ ﻪﺘﻓﺎﻳ ﺶﻳاﺰﻓا ﺎﻫ
. نآ ﺮﺑ هوﻼﻋ
ﻲﻨﻌﻣ فﻼﺘﺧا ﻪﻛ ﻦﻳا ﺎﺑ ياﻮﺘﺤﻣ توﺎﻔﺗ درﻮﻣ رد يراد
هوﺮﮔ رد ،ﺪﻣﺎﻴﻧ ﺖﺳد ﻪﺑ ﻪﻳر نزو ﺪﺣاو رد ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ﻲﻔﻨﻣ ﺪﻫﺎﺷ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ﻲﻬﺟﻮﺗ ﻞﺑﺎﻗ ﺶﻳاﺰﻓا ،ﺖﺒﺜﻣ ﺪﻫﺎﺷ ﺪﺷ هﺪﻫﺎﺸﻣ .
ﻪﻳر ﺖﻓﺎﺑ ﻦﻣﺰﻣ بﺎﻬﺘﻟا زوﺮﺑ ﺮﺑ ّلاد ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ
زوﺮﺑ و نژﻼﻛ بﻮﺳر عوﺮﺷ ًﻻﺎﻤﺘﺣا و ﻲﻣ يﻮﻳر زوﺮﺒﻴﻓ
ﺪﺷﺎﺑ .
راﺪﻘﻣ رﺎﻈﺘﻧا فﻼﺧ ﺮﺑ نﺎﻣﺰﻤﻫ H2O2
شﻮﻣ مﺮﺳ يﺎﻫ
ﻲﻨﻌﻣ ﺶﻫﺎﻛ ،ﺖﺒﺜﻣ ﺪﻫﺎﺷ نﺎﺸﻧ ﻢﻟﺎﺳ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ار يراد
داد . ﺶﻨﻛاو ﻖﻳﺮﻃ زا نژورﺪﻴﻫ ﺪﻴﺴﻛاﺮﭘ ﻪﻛ ﻦﻳا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
زﺎﺗﻮﻤﺴﻳدﺪﻴﺴﻛاﺮﭘﻮﺳ ﻢﻳﺰﻧآ ﻂﺳﻮﺗ نﻮﻴﺳﺎﺗﻮﻤﺴﻳد (SOD)
ﻲﻣ ﺪﻴﻟﻮﺗ دﻮﺷ
) 15 ( راﺪﻘﻣ ﺶﻫﺎﻛ،
H2O2 ار ﻲﻣ زا ﻲﺷﺎﻧ ناﻮﺗ
ﻢﻳﺰﻧآ دﺮﻜﻠﻤﻋ ﺺﻘﻧ ﺎﻳ و دﻮﺒﻤﻛ ﺪﻴﺴﻛاﺮﭘﻮﺳ
زﺎﺗﻮﻤﺴﻳد ﺖﺴﻧاد
ﻦﻳﺮﺘﻤﻬﻣ ﻪﻛ ﺪﻴﺴﻛا ﺮﭘﻮﺳ نﻮﻴﻧآ ﺖﻈﻠﻏ ﻦﺘﻓر ﻻﺎﺑ ﻪﺑ ﺮﺠﻨﻣ ﻪﻛ ﺖﺳا نﺪﺑ رد نﮋﻴﺴﻛا ﺖﻴﻤﺳ ﻞﻣﺎﻋ )
16 ( ﻲﻣ ، دﻮﺷ .
ﻪﻌﻟﺎﻄﻣ رد Husain
ﺎﺑ دﺎﺣ ﺔﻬﺟاﻮﻣ ،نارﺎﻜﻤﻫ و زﺎﮔ
لدﺮﺧ ﺖﻴﻟﺎﻌﻓ ﺶﻫﺎﻛ ﻪﺑ ﺮﺠﻨﻣ ، ﺪﻴﺴﻛاﺮﭘﻮﺳ
ﺗﻮﻤﺴﻳد زﺎ رد
لﻮﺒﻠﮔ ﺖﻛﻼﭘ ،ﺪﻴﻔﺳ يﺎﻫ لﻮﻠﺳ ،ﺎﻫ
ناﻮﺨﺘﺳا ﺰﻐﻣ و لﺎﺤﻃ يﺎﻫ
ﺪﺷ ﻲﻧاﻮﻴﺣ لﺪﻣ رد )
17 ( ﻞﺧاد ﺰﻳﻮﺠﺗ يﺮﮕﻳد ﻪﻌﻟﺎﻄﻣ رد ؛
ﻲﻗﺎﻔﺻ ﺪﻴﺴﻛاﺮﭘﻮﺳ زﺎﺗﻮﻤﺴﻳد
،ﻲﺘﺳﻮﭘ يﺎﻬﻤﺧز دﺎﺠﻳا زا ﻞﺒﻗ
هدﺎﻌﻟا قﻮﻓ ترﻮﺻ ﻪﺑ ﺮﺛا رد ﻲﮕﺘﺧﻮﺳ ﻪﻘﻄﻨﻣ ﺖﻌﺳو ،يا
زﺎﮔ
لدﺮﺧ داد ﺶﻫﺎﻛ ار )
18 ( ﻪﻌﻟﺎﻄﻣ ﻪﺠﻴﺘﻧ ﻪﺘﺒﻟا ؛ Buezynski
و Gnitecki ﺖﻴﻟﺎﻌﻓ ﺶﻳاﺰﻓا ،قﻮﻓ دراﻮﻣ فﻼﺧ ﺮﺑ
ﺪﻴﺴﻛاﺮﭘﻮﺳ
زﺎﺗﻮﻤﺴﻳد لﻮﻠﺳ رد ار
ﻪﻬﺟاﻮﻣ زا ﺲﭘ ﺖﻛﻼﭘ يﺎﻫ In-vitro
ﺎﺑ
لدﺮﺧ زﺎﮔ داد نﺎﺸﻧ
) 19 .(
هﺪﺷ حﺮﻄﻣ ﻪﻴﺿﺮﻓ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﻲﻣ دﺎﻬﻨﺸﻴﭘ ﺮﻳدﺎﻘﻣ يﺪﻌﺑ تﺎﻌﻟﺎﻄﻣ رد ددﺮﮔ
ﺪﻴﺴﻛاﺮﭘﻮﺳ
زﺎﺗﻮﻤﺴﻳد لﺪﻣ رد مﺮﺳ ﺪﻴﺴﻛاﺮﭘﻮﺳ لﺎﻜﻳدار و
ضراﻮﻋ سرﺮﻳد
زا ﻲﺷﺎﻧ لدﺮﺧ زﺎﮔ
هزاﺪﻧا ﺶﻳاﺰﻓا ﺐﻴﺗﺮﺗ ﻦﻳا ﻪﺑ ؛دﻮﺷ يﺮﻴﮔ
ﻦﻴﻣﺎﺘﻳو ﺎﻴﺣا مﺮﻓ ﺶﻫﺎﻛ ﺚﻋﺎﺑ ﻮﻴﺗاﺪﻴﺴﻛا ﺶﻨﺗ ﻲﻣC
ﻪﻛ ددﺮﮔ
ﻲﻣ هﺪﻫﺎﺸﻣ ﺰﻴﻧ ﺞﻳﺎﺘﻧ رد ﻦﻴﻣﺎﺘﻳو ﻪﻛ ﺎﺠﻧآ زا و دﻮﺷ
C
ﻲﺘﻧآ هدﺎﻣ ﻦﻳﺮﺘﻤﻬﻣ ﺖﺳا ﻲﻳاﻮﻫ يﺎﻬﻫار رد دﻮﺟﻮﻣ ناﺪﻴﺴﻛا
) 20 (
ﺟﻮﺗ ﻞﺑﺎﻗ بﺮﺨﻣ رﺎﺛآ نآ دﻮﺒﻤﻛ ، ﻲﻣ ﻲﻬ
ﺪﺷﺎﺑ ﻪﺘﺷاد ﺪﻧاﻮﺗ .
،ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد شﻮﻣ رد ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﺰﻳﻮﺠﺗ
ﺎﻫ ي ﻪﻬﺟاﻮﻣ ﺎﺑ ﻪﺘﻓﺎﻳ لدﺮﺧ زﺎﮔ
، ﻨﻌﻣ ﺶﻫﺎﻛ ﻪﺑ ﺮﺠﻨﻣ ﻲ
ﻘﻣ راد ﺪ ا ر
شﻮﻣ ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ﺎﻫ
ﺶﻫﺎﻛ و ﻌﻣﺮﻴﻏ
ﻲﻨ راد حﺎﺸﺗرا
ﺖﻴﺳﻮﻔﻨﻟ ﺎﻫ ﺶﻳاﺰﻓا ﻦﻴﻨﭽﻤﻫ و ﻨﻌﻣ
ﻲ راد ﺮﻳدﺎﻘﻣ H2O2 و
ﻦﻴﻣﺎﺘﻳو مﺮﺳ C
ﺷ ﺪ
؛
ﺪﺣ ﻪﺑ ي ﻪﻛ ﺮﻳدﺎﻘﻣ H2O2 ﻦﻴﻣﺎﺘﻳو و C
ﻪﻧﻮﻤﻧ ﺎﺑ يﺎﻫ ﻢﻟﺎﺳ ﻨﻌﻣ توﺎﻔﺗ ﻲ
راد ي ﺪﻧ نﺎﺸﻧ ار اد
؛ ﻦﻳاﺮﺑﺎﻨﺑ
ﻲﻣ ﻪﻛ ﺖﻔﮔ ناﻮﺗ ﺪﺿ ﺮﺛا ﺮﺑ هوﻼﻋ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ
زوﺮﺒﻴﻓ ي
،
اراد ﺖﺳا ﻦﻜﻤﻣ ي
ﺪﺿ تاﺮﺛا ﻲﺑﺎﻬﺘﻟا
ﺪﺷﺎﺑ . ﺮﺑ ﺞﻳﺎﺘﻧ سﺎﺳا
ﻪﻌﻟﺎﻄﻣ Siwik نارﺎﻜﻤﻫ و
، H2O2 نژﻼﻛ ﺰﺘﻨﺳ ﺶﻫﺎﻛ ﺚﻋﺎﺑ
ﺳﻼﺑوﺮﺒﻴﻓ رد رﻼﻳﺮﺒﻴﻓ ﺖ
ﺐﻠﻗ يﺎﻫ ﻲﻣ
دﻮﺷ ) 21 (
؛ ﺐﻴﺗﺮﺗ ﻦﻳا ﻪﺑ
ﻲﻣ شﻮﻣ ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ياﻮﺘﺤﻣ ﺶﻫﺎﻛ ﺖﻠﻋ ناﻮﺗ ﺎﻫ
ﺶﻳاﺰﻓا زا ﻲﺷﺎﻧ ار H2O2
ﺖﺴﻧاد .
ﺖﻴﺳﻮﻔﻨﻟ ﺶﻘﻧ ﺮﮕﻳد ﻪﻴﺿﺮﻓ ﺎﻫ
ي رد T اﺮﻓ ﻳ ﺪﻨ دﺎﺠﻳا
زوﺮﺒﻴﻓ ﻲﻣ ﺪﺷﺎﺑ . ﻪﻌﻟﺎﻄﻣ ﺞﻳﺎﺘﻧ Sendelbach
نارﺎﻜﻤﻫ و
نﺎﺸﻧ ﺖﻴﺳﻮﻔﻨﻟ ﻪﻛ ﺖﺳا ﺐﻠﻄﻣ ﻦﻳا هﺪﻨﻫد ﺎﻫ
ي رد T ﺪﻧور
زا ﻲﺷﺎﻧ زوﺮﺒﻴﻓ دﺎﺠﻳا Butylated Hydroxytoloen
،
ﻣ مﻮﻴﻟﺮﺑ و ﻦﻴﺴﻳﺎﻣﻮﺌﻠﺑ ﺆ
ﺮﺛ ﻲﻣ ﺪﻨﺷﺎﺑ ) 22 ( ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ و ﺎﺑA
ﺎﻬﺨﺳﺎﭘ فﺬﺣ ي
ﺖﻴﺳﻮﻔﻨﻟ ﻪﺑ طﻮﺑﺮﻣ ﻲﺑﺎﻬﺘﻟا ﺎﻫ
ي ﺚﻋﺎﺑ T
اﻮﺘﺤﻣ ﺶﻫﺎﻛ ي
ﻪﻳر ﻦﻴﻟوﺮﭘ ﻲﺴﻛورﺪﻴﻫ ﻲﻣ
دﻮﺷ . نﺎﻴﻣ ﻦﻳا رد
ﻢﻫ زﺎﺑ ﻲﻣ ﺶﻘﻧ ناﻮﺗ H2O2
ار ﺑ ﻪ ﻜﻠﻤﻋ ﻪﻄﺳاو ناﻮﻨﻋ دﺮ
حﺮﻄﻣ ﻦﻳرﻮﭙﺳﻮﻠﻜﻴﺳ ﻮﻤﻧ
د . اﺮﺑ ي مﺎﺠﻧا ﻪﺑ ﻪﻴﺿﺮﻓ ﻦﻳا تﺎﺒﺛا
ﺮﺘﺸﻴﺑ تﺎﻌﻟﺎﻄﻣ ي
ﺖﺳا زﺎﻴﻧ .
ﻮﺴﻤﻫ ﻳ ﻲ ﻦﻴﻣﺎﺘﻳو تاﺮﻴﻴﻐﺗ و C
H2O2
رد تﺎﻌﻟﺎﻄﻣ
دﺪﻌﺘﻣ ي ﺖﺳا هﺪﺷ هﺪﻫﺎﺸﻣ
؛ ﺑ ﻪ ﻪﻌﻟﺎﻄﻣ رد لﺎﺜﻣ ناﻮﻨﻋ
Mendiratta نارﺎﻜﻤﻫ و
، لﻮﺒﻠﮔ رد ﺎﻫ
ي ﻚﻴﺑرﻮﻜﺳاﺪﻴﺳا ﺰﻣﺮﻗ
ﺮﭘ ناﺰﻴﻣ رد زود ﻪﺑ ﻪﺘﺴﺑاو ﺶﻳاﺰﻓا نژورﺪﻴﻫ ﺪﻴﺴﻛا
نورد
ﻲﻟﻮﻠﺳ ﻲﻣ ﺪﻫد ) 23 ( ﻦﻴﻣﺎﺘﻳو ﻲﻗﺎﻔﺻ ﻖﻳرﺰﺗ ﺎﻳ و ﻪﺑ C
شﻮﮔﺮﺧ
، راﺪﻘﻣ H2O2 ﺶﻳاﺰﻓا ار نآ ﻢﺸﭼ ﻪﻴﻟﻻز ﻲﻣ
ﺪﻫد
) 24 ( . ﺮﮕﻳد ﺮﻛذ ﻞﺑﺎﻗ ﻪﺘﻜﻧ نﺎﺸﻧ ﻦﻴﺸﻴﭘ تﺎﻌﻟﺎﻄﻣ ﻪﻛ نآ
هﺪﻨﻫد
وراد ﺮﺛا ي دﺎﺠﻳا رد رﻮﻛﺬﻣ ﺶﻨﺗ
ﺖﺳا ﻮﻴﺗاﺪﻴﺴﻛا )
25 - 27 ( ﻪﻛ
ﺪﻨﻳﺎﺷﻮﺧﺎﻧ ضراﻮﻋ دﺎﺠﻳا ﻪﺑ ﺮﺠﻨﻣ نآ
ﻲﻣ ﻪﻌﻟﺎﻄﻣ رد ﺎﻣا ددﺮﮔ
ﺖﻈﻠﻏ ﺮﺿﺎﺣ H2O2
توﺎﻔﺗ ﻲﻨﻌﻣ راد ي ﺖﺷاﺪﻧ ﻢﻟﺎﺳ هوﺮﮔ ﺎﺑ
؛
١٠
ﺪﺣ ﻪﺑ نآ ﺪﻴﻟﻮﺗ ﻲﻨﻌﻳ ي
ﻲﻳوراد ضراﻮﻋ ﻪﺑ ﺮﺠﻨﻣ ﺪﻧاﻮﺘﺑ ﻪﻛ
ددﺮﮔ
، دﻮﺑ ﻪﺘﻓﺎﻴﻧ ﺶﻳاﺰﻓا .
ﻪﺠﻴﺘﻧ يﺮﻴﮔ
لدﺮﺧ زﺎﮔ ﺶﻳاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ ﺰﻴﻧ دﻮﺧ سرﺮﻳد ﺐﻴﺳآ رد
ﺶﻨﺗ ﻮﻴﺗاﺪﻴﺴﻛا ﻲﻣ
ﻞﺑﺎﻘﻣ رد و ددﺮﮔ ﻳرﻮﭙﺳﻮﻠﻜﻴﺳ
ﻦ
ﺪﺷ زا ت
ﺶﻨﺗ زا ﻲﺷﺎﻧ ﻮﻴﺗاﺪﻴﺴﻛا لدﺮﺧ زﺎﮔ
ﺎﻛ ﻪﺘﺳ ﻖﻳﺮﻃ ﻦﻳا زا و
ﺛا ﺮﻫﺎﻇ ار دﻮﺧ ﻲﻧﺎﻣرد تاﺮ ﻲﻣ
زﺎﺳ د . اﺮﺑ
ي دﺎﻬﻨﺸﻴﭘ ﺐﻠﻄﻣ ﻦﻳا ﺮﺘﻘﻴﻗد ﻲﺳرﺮﺑ ﻲﻣ
رد ﻪﻛ دﻮﺷ
ﺮﻳدﺎﻘﻣ هﺪﻨﻳآ تﺎﻌﻟﺎﻄﻣ ﺴﻛاﺮﭘﻮﺳ
ﻴ ﺪ د ﻳ زﺎﺗﻮﻤﺴ لﺎﻜﻳدار و ﺎﻫ
ي
ﺎﺑ ﻪﻬﺟاﻮﻣ زا ﺲﭘ ﺪﻴﺴﻛاﺮﭘﻮﺳ و ﻞﻴﺴﻛورﺪﻴﻫ زﺎﮔ
لدﺮﺧ ﺪﻌﺑ و
ﻣرد زا وراد ﺎﺑ نﺎ ي
هزاﺪﻧا رﻮﻛﺬﻣ ﺮﻴﮔ
ي ددﺮﮔ .
ﺮﻜﺸﺗ و ﺮﻳﺪﻘﺗ
زا قﺮﺷ نﺎﻤﻴﻜﺣ ﻲﺗﺎﻘﻴﻘﺤﺗ ﺖﻛﺮﺷ ﻦﻳا ﻲﻟﺎﻣ ﺖﻳﺎﻤﺣ ﻪﻛ
ﻲﻣ ﻲﻧادرﺪﻗ و ﺮّﻜﺸﺗ ،ﺪﻨﺘﺷاد هﺪﻬﻋ ﺮﺑ ار ﻖﻴﻘﺤﺗ ددﺮﮔ
.
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Fundam Clin Pharmacol. 2005; 19 (3): 297-315.
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١٢ Therapeutic effect of cyclosporine on delayed pulmonary
complication due to mustard gas in mice
F. Ghassami1, L. Ansari1, Sh. Moradi2; N. Afshar Moghaddam3, N. Mirkheshti4, SA. Alavi5
Abstract
Background and Aim: Among late complications in mustard gas or sulfur mustard (SM) casualties, respiratory problems, especially chronic bronchitis and pulmonary fibrosis are the greatest causes of long- term disability. Cyclosporine has anti-inflammatory effects and is used in other pulmonary fibroses due to other factors. Thus, with respect to the role of oxidative stress in the pathogenesis of chronic bronchitis and pulmonary fibrosis, the therapeutic effects of Cyclosporine on delayed complications of mustard gas and the role of oxidative stress in the procedure were studied.
Materials and Methods: In this experimental study, a group of six male mice received cyclosporine after five months of inhaling mustard gas. After killing the mice and obtaining tissue samples, biochemical analyses of hydroxyproline of lung, H2O2, vitamin C of serum, and also alveolar space percentage and inflammatory cell infiltration, the comparison between positive and negative control and also between case and positive control was performed.
Results: Results indicated a significant decrease in alveolar space percent, lymphocyte infiltration, H2O2, and vitamin C in the positive control group. Cyclosporine administration decreased lung hydroxyproline and lymphocyte infiltration; and normalized H2O2 and vitamin C.
Conclusion: Decrease of pulmonary alveolar space of the mice brings up two hypotheses. First: collagen precipitation and incidence of pulmonary fibrosis. Second: inflammatory cell infiltration and incidence of chronic inflammation that is corresponding to SM induced lung injury in chemical casualties. Decrease of H2O2 -that can be due to decrease of superoxide dismutase- and vitamin C, demonstrates decrease of antioxidant defense in the serum of the mice. Cyclosporine administration effects by normalizing these two agents-; i.e decreasing oxidative stress- induces a decrease in lung hydroxyproline (index of fibrosis) and lymphocyte infiltration (index of chronic tissue inflammation).
Key Words: Mustard gas; Oxidative stress; Cyclosporine; Delayed complications
1 Student of Medicine, Isfahan University of Medical Sciences. Isfahan, Iran.
2 B.Sc in Chemistry
3 Assistant Professor, Department of Pathology, Faculty of Medicine, Isfahan University of Medical Sciences. Isfahan, Iran.
4 Physician
5 Corresponding Author; Physician. [email protected]
