نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺮﺛا
لﻮﻜﻴﻟﻮﻓ ﻦﻳﻮﻜﺗ ﺮﺑ داﮋﻧ شﻮﻣ ﻲﻧاﺪﻤﺨﺗ يﺎﻫ
Balb/c
رﺎﻬﺑ داﻮﺟ آ
1ار يﻮﺳﻮﻣ ﺖﻫﺰﻧ ،
2ﺮﻓ ﻲﻟﻼﺟ ﻦﺴﺤﻣ ،
،2
لﺪﻋ ﻪﻧازﺮﻓ 3
هﺪﻴﻜﭼ
ﻪﻨﻴﻣز فﺪﻫ و يرورﺎﺑﺎﻧ و ﻞﺜﻣ ﺪﻴﻟﻮﺗ تﺎﻌﻟﺎﻄﻣ رد : ﺎﻄﻣ ﺰﻴﻧ و
ﻟ ﻲﻜﻴﺘﻧژ تﺎﻌ
، شور ﺮﺤﺗ ﻳ ﻚ ﻧاﺪﻤﺨﺗ ﻲ لﺮﺘﻨﻛ هﺪﺷ ﻔﺘﺳا ﺎﺑ زا هدﺎ
ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺎﻫ
، رادرﻮﺧﺮﺑ ﻲﺻﺎﺧ ﺖﻴﻤﻫا زا ﺖﺳا
. ﭘوﺮﺗودﺎﻨﮔ نﻮﻣرﻮﻫ ﺮﺛا ﺮﺿﺎﺣ ﺶﻫوﮋﭘ رد ﻴ
ﻦ ﺘﻔﺟ ﻲ نﺎﺴﻧا
hCG ) ( ﺮﺑ ﻮﻜﺗ ﻳ ﻦ
ﻟﻮﻓ ﻴ لﻮﻜ ﺎﻫ ي ﻧاﺪﻤﺨﺗ ﻲ داﮋﻧ شﻮﻣ
Balb/c
رﺮﺑ ﺳ ﻲ ﺪﺷ .
شور ﻖﻴﻘﺤﺗ ﻲﺑﺮﺠﺗ ﺶﻫوﮋﭘ ﻦﻳا رد :
، 30 دﺎﻣ شﻮﻣ ﺮﺳ ه
هﺮﻛﺎﺑ 21 داﮋﻧ هزور
Balb/c
هوﺮﮔ ﻪﺳ ﻪﺑ و بﺎﺨﺘﻧا ﻫﺎﺷ
ﺪ
، ﺪﻫﺎﺷ
ﻲﻫﺎﮕﺸﻳﺎﻣزآ )
ﻖﻳرﺰﺗ 1 / 0 ﻲﻠﻴﻣ ﺮﺘﻴﻟ ﻦﻴﻟﺎﺳ لﺎﻣﺮﻧ ( ﻲﺑﺮﺠﺗ و ) ﻖﻳرﺰﺗ 1 / 0 ﻲﻠﻴﻣ نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ نﻮﻣرﻮﻫ ﺮﺘﻴﻟ
IU/ml
5 ( ﻢﻴﺴﻘﺗ
ﺪﻧﺪﺷ . شﻮﻣ ﺎﻫ 18 ﺪﺷ ﻪﺘﺸﻛ مﺮﻓوﺮﻠﻛ ﻂﺳﻮﺗ ﻖﻳرﺰﺗ زا ﺲﭘ ﺖﻋﺎﺳ ﺪﻧ
ناﺪﻤﺨﺗ و ﺎﻫ جرﺎﺧ ﺎﻬﻧآ ي و
ﻲﺳرﺮﺑ ﺎﻫ ﺖﺨﻳر ﻪﻴﻟوا ي ﺖﺒﺛ و ﻲﺳﺎﻨﺷ
هزاﺪﻧا و نزو ﻧا
ﺪﺷ مﺎﺠ
؛ ﺲﭙﺳ لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ يرﻮﻧ ﻲﭘﻮﻜﺳوﺮﻜﻴﻣ تﺎﻌﻟﺎﻄﻣ ﻚﻤﻛ ﻪﺑ ﺎﻫ
رد ،ﻪﻳﻮﻧﺎﺛ ،ﻪﻴﻟوا ي و ﺪﺷر لﺎﺣ
ﺰﻴﻧ لﻮﻜﻴﻟﻮﻓ ﺎﻫ ي
ﻪﻴﻟوا لاﺮﺘﻧآ ﻪﻧﻮﻤﻧ ﺮﻫ
ﻲﺳرﺮﺑ و ﺪﺷ ﻪﺴﻳﺎﻘﻣ .
هداد ﺎﻫ زا هدﺎﻔﺘﺳا ﺎﺑ مﺮﻧ
راﺰﻓا و SPSS
نﻮﻣزآ ﺎﻫ يرﺎﻣآ ي
ANOVA
و
Kolmogorov-Smirnov
ﻲﻨﻌﻣ ﺢﻄﺳ رد يراد
05 /
P<0 ﺠﺗ ﺪﻧﺪﺷ ﻞﻴﻠﺤﺗ و ﻪﻳﺰ .
ﻪﺘﻓﺎﻳ ﺎﻫ ﻲﻨﻌﻣ توﺎﻔﺗ: لﻮﻜﻴﻟﻮﻓ ﻂﺳﻮﺘﻣ داﺪﻌﺗ ،ناﺪﻤﺨﺗ هزاﺪﻧا ﺮﻈﻧ زا هوﺮﮔ ﻪﺳ ﻦﻴﺑ يراد
ﺪﺸﻧ هﺪﻫﺎﺸﻣ ﻪﻳﻮﻧﺎﺛ و ﻪﻴﻟوا يﺎﻫ )
05 /
P>0 (.
ﻖﻳرﺰﺗ ﺶﻳاﺰﻓا ﺚﻋﺎﺑhCG
ﻲﻨﻌﻣ رد يراد ناﺪﻤﺨﺗ نزو ﺎﻫ
داﺪﻌﺗ و ﻂﺳﻮﺘﻣ لﻮﻜﻴﻟﻮﻓ ﺎﻫ لﻮﻜﻴﻟﻮﻓ و ﺪﺷر لﺎﺣ رد ي ﺎﻫ
ﻪﻴﻟوا لاﺮﺘﻧآ ي رد
ﻘﻣ ﺪﺷ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ و ﺪﻫﺎﺷ هوﺮﮔ ﺎﺑ ﻪﺴﻳﺎ )
001 /
P=0 (.
ﻪﺠﻴﺘﻧ يﺮﻴﮔ ﻪﺘﻓﺎﻳ سﺎﺳا ﺮﺑ :
،ﻪﻌﻟﺎﻄﻣ ﻦﻳا يﺎﻫ ﺮﺛا
نﻮﻣرﻮﻫ ﭘوﺮﺗودﺎﻨﮔ ﻴ ﻦ ﺘﻔﺟ ﻲ نﺎﺴﻧا لﻮﻜﻴﻟﻮﻓ ﺪﺷر ناﺰﻴﻣ ﺶﻳاﺰﻓا يور ﺮﺑ يﺎﻫ
ﻲﻣ ﺮﺘﺸﻴﺑ ﻲﻳﺎﻬﺘﻧا ﻞﺣاﺮﻣ رد ،ناﺪﻤﺨﺗ ﺪﺷﺎﺑ
.
هژاو يﺪﻴﻠﻛ يﺎﻫ
،نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘﺮﺗودﺎﻨﮔ: ،ناﺪﻤﺨﺗ
Balb/c شﻮﻣ ، ﻦﻳﻮﻜﺗ ﻲﻟﻮﻜﻴﻟﻮﻓ
ﺪﻨﺟﺮﻴﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻤﻠﻋ ﻪﻠﺠﻣ .
1389
؛ 17 ) 4 :(
274 - 280
ﺖﻓﺎﻳرد : 27 / 05 / 1388 ﺎﻬﻧ حﻼﺻا ﻲﻳ : 13 / 07 / 1389 شﺮﻳﺬﭘ : 04 / 08 / 1389
؛لوﺆﺴﻣ هﺪﻨﺴﻳﻮﻧ1
رﺎﻳدﺎﺘﺳا ﺖﺴﻳز هوﺮﮔ ﻲﺳﺎﻨﺷ
، ﻪﻳﺎﭘ مﻮﻠﻋ هﺪﻜﺸﻧاد
، ﺪﻬﺸﻣ ﺪﺣاو ﻲﻣﻼﺳا دازآ هﺎﻜﺸﻧاد
، ناﺮﻳا
سردآ : ﺪﻬﺸﻣ - دﺎﺑآ ﻢﺳﺎﻗ - ﻪﻴﻣﺎﻣا 42 - ﺪﻬﺸﻣ ﺪﺣاو ،ﻲﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﻲﺸﻫوﮋﭘ ﺖﻧوﺎﻌﻣ هزﻮﺣ
ﻦﻔﻠﺗ : 6223137 - 0511 ﺮﺑﺎﻤﻧ : 6228310 - 0511 ﻲﻜﻴﻧوﺮﺘﻜﻟا ﺖﺴﭘ [email protected]:
رﺎﻳدﺎﺘﺳا2
ﻪﻳﺮﺼﺘﻨﻣ يرورﺎﺑﺎﻧ ﻲﺗﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ
، ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ناﺮﻳا ،
ﺷرا سﺎﻨﺷرﺎﻛ3
ﺪﻬﺸﻣ ﺪﺣاو ﻲﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﻪﻳﺎﭘ مﻮﻠﻋ هﺪﻜﺸﻧاد ﻲﺳﺎﻨﺷ ﺖﺴﻳز هوﺮﮔ ﻲﻨﻳﻮﻜﺗ ﻲﻟﻮﻠﺳ ﺪ
ﻪﻣﺪﻘﻣ و يرورﺎﺑﺎﻧ و ﻞﺜﻣ ﺪﻴﻟﻮﺗ تﺎﻌﻟﺎﻄﻣ رد ﻴﻟﻮﺗ
ﻪﻧﻮﻤﻧ ﺪ ﺎﻫ ي
ﻲﻜﻴﺘﻧژ ﺪﻳﺪﺟ
، ﻚﻤﺨﺗ داﺪﻌﺗ ﺶﻳاﺰﻓا و ﻲﻧاﺪﻤﺨﺗ ﻚﻳﺮﺤﺗ ﺎﻫ
ي
ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﻂﺳﻮﺗ سﺮﺘﺳد ﻞﺑﺎﻗ ﺎﻫ
رادرﻮﺧﺮﺑ يدﺎﻳز ﺖﻴﻤﻫا زا
ﺖﺳا . ﻦﻳا ياﺮﺑ ﻲﺒﺳﺎﻨﻣ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﻪﻧﻮﻤﻧ شﻮﻣ ﻪﻧﻮﮔ
ﺶﻫوﮋﭘ ﺎﻫ ﻲﻣ ﺪﺷﺎﺑ . لﻮﻜﻴﻟﻮﻓ ﺎﻫ ﻲﻧاﺪﻤﺨﺗ ي
، ار دﻮﺧ ﻮﻤﻧ و ﺪﺷر
ﻪﻴﻟوا يﺎﻫرﺎﺘﺧﺎﺳ ناﻮﻨﻋ ﻪﺑ ﺖﻴﺳووا ﻚﻳ يوﺎﺣ ﻪﻛ يا
ﻒﻗﻮﺘﻣ زﻮﻴﻣ ﻢﻴﺴﻘﺗ ﻦﻴﻟوا ﻦﺗﻮﻠﭙﻳد ﺔﻠﺣﺮﻣ رد هﺪﺷ ﻲﻣ
ﺪﻨﺷﺎﺑ رد ،
زﺎﻏآ ﻢﺋﻼﻋ زا يﺮﺳ ﻚﻳ ﻪﺑ ﺦﺳﺎﭘ ﻲﻣ
ﺪﻨﻳﺎﻤﻧ ) 1 (.
ﻲﻣﺎﮕﻨﻫ ﻪﻛ
لﻮﻜﻴﻟﻮﻓ ﺎﻫ زﺎﻏآ ار دﻮﺧ ﺪﺷر ﻲﻣ
ﺪﻨﻨﻛ
، لﻮﻠﺳ ﺎﻫ هزﻮﻟﻮﻧاﺮﮔ ي
ﺺﺧﺎﺷ و هﺪﺷ ﻲﺒﻌﻜﻣ ﺎﻫ
نﺎﺸﻧ ار ﻲﻟﻮﻠﺳ ﺮﻴﺜﻜﺗ ي ﻲﻣ
ﺪﻨﻫد
) 2 .(
ﻲﺧﺮﺑ هﺪﺷﺮﺸﺘﻨﻣ ﻲﻤﻠﻋ تﺎﺷراﺰﮔ ﻖﺒﻃ ﻞﻣاﻮﻋ
ﻪﻠﻤﺟ زا
نﻮﻣرﻮﻫ FSH يﺎﻫ
،1
2GDF
βFGF و ،3
لﻮﻜﻴﻟﻮﻓ ﺪﺷر ﺎﻫ
ي
ﻚﻳﺮﺤﺗ ار ﻪﻴﻟوا ﻲﻣ
نﻮﻣرﻮﻫ زا ﺮﮕﻳد ﻲﺧﺮﺑ و ﺪﻨﻨﻛ ﺎﻫ
لﺎﻘﺘﻧا
ﻚﻳﺮﺤﺗ ار ﻪﻳﻮﻧﺎﺛ ﻪﺑ ﻪﻴﻟوا لﻮﻜﻴﻟﻮﻓ ﻲﻣ
ﺪﻨﻨﻛ
؛ ﻦﻳا ﺐﻠﻏا
ﺖﺳا هﺪﺷ ﻪﺋارا نﺎﮔﺪﻧﻮﺟ درﻮﻣ رد تﺎﺷراﺰﮔ )
3 .(
ﻞﻣﺎﻋ ﺪﺷر
لﻮﻜﻴﻟﻮﻓ ﺪﺷر ،ﻲﻣرﺪﻴﭘا ﺎﻫ
يﺮﭘ ي و لاﺮﺘﻧآ ﻪﺑ نآ ﻞﻳﺪﺒﺗ
ﺶﻳاﺰﻓا ار ﻪﻴﻟوا لاﺮﺘﻧآ لﻮﻜﻴﻟﻮﻓ ﻲﻣ
ﺪﻫد ) 4 ( . ﻦﻴﭘوﺮﺗودﺎﻨﮔ
نﺎﺴﻧا ﻲﺘﻔﺟ hCG )
4( نﻮﻣرﻮﻫ ةداﻮﻧﺎﺧ زا ﻮﻀﻋ ﻚﻳ ﺎﻫ
ﺖﺳا
ﺎﻘﻟا ياﺮﺑ نآ ﻖﻳرﺰﺗ ﻪﻛ ي
يرورﺎﺑﺎﻧ نﺎﻣرد رد نﻮﻴﺳﻻووا
دراد دﺮﺑرﺎﻛ )
5 .(
ﻦﻴﭘوﺮﺗودﺎﻨﮔ تاﺮﺛا ﺎﻫ
ﻲﻫﺎﮕﺸﻳﺎﻣزآ غﻮﻠﺑ ﺮﺑ
ﺖﻴﺳووا ﺎﻫ ﻲﻧﺎﺴﻧا ﻎﻟﺎﺑﺎﻧ ي رد
ﻌﻟﺎﻄﻣ تﺎ يدﺎﻳز ﺪﺷ ﻲﺳرﺮﺑ ه
ﺖﺳا ) 7،6 (.
ﻪﻌﻟﺎﻄﻣ Chian نارﺎﻜﻤﻫ و ﻪﻛ ﺖﺳا هداد نﺎﺸﻧ
نﺎﻧز رد ﻪﺑ ﻼﺘﺒﻣ
ناﺪﻤﺨﺗ مرﺪﻨﺳ ﻲﻠﭘ
ﻖﻳرﺰﺗ ،ﻚﻴﺘﺴﻴﻛ
ﺶﻳاﺰﻓا ﺚﻋﺎﺑ ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺖﻴﺳووا غﻮﻠﺑ
ﺎﻫ
ﻲﻃ رد 48
ﺖﺸﻛ لوا ﺖﻋﺎﺳ ﻲﻣ
دﻮﺷ ) 8 .(
ﺮﺑ ﻪﻌﻟﺎﻄﻣ ﺖﻴﺳووا يور
ﺎﻫ ي
داد نﺎﺸﻧ ﮓﺳ ﻪﻛ
ﻂﻴﺤﻣ رد نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ دﻮﺟو
ﻚﻤﺨﺗ داﺪﻌﺗ ﺶﻳاﺰﻓا ﺐﺒﺳ ،ﺖﺸﻛ ﺎﻫ
ﻎﻟﺎﺑ ي ﻲﻣ
دﻮﺷ ) 9 (
؛
ﻦﻴﻨﭽﻤﻫ ﺖﺸﻛ ﻂﻴﺤﻣ ﻪﺑ نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ندوﺰﻓا
ﻈﺣﻼﻣ ﻞﺑﺎﻗ ﺶﻳاﺰﻓا ﺐﺒﺳ ﻪ
ﺖﻴﺳووا ﺎﻫ نﺎﺴﻧا ﻎﻟﺎﺑ ي ﻲﻣ
دﻮﺷ
Follicle Stimulating Hormone١
٢
Growth differentiation factor
Fibroblastic Growth Factor٣ Human Chorionic Gonadotropin٤
) 10 .(
تﺎﻌﻟﺎﻄﻣ ﺮﻳﺎﺳ ﺮﺑ
يور ﺖﻴﺳووا ﺎﻫ ﺎﻣﺮﺧ شﻮﻣ ي و
كﻮﺧ
ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ دﺮﺑرﺎﻛ ﻪﻛ ﺖﺳا هداد نﺎﺸﻧ
، ﺶﻳاﺰﻓا ﺐﺒﺳ
ﺖﻴﺳووا غﻮﻠﺑ ناﺰﻴﻣ ﺎﻫ
ﻲﻣ دﻮﺷ ) 12،11 (.
تﺎﻌﻟﺎﻄﻣ ﺮﻳﺎﺳ ﺞﻳﺎﺘﻧ
ﺰﻴﻧ ﺎﻘﻟا رد نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺪﻴﻔﻣ تاﺮﺛا ﺮﮕﻧﺎﻴﺑ ي
لﻮﻜﻴﻟﻮﻓ ﻚﻤﺨﺗ و ﻲﻳاز نارﺎﻤﻴﺑ رد يراﺬﮔ
مرﺪﻨﺳ ياراد
ناﺪﻤﺨﺗ ﻲﻠﭘ ﺖﺳا ﻚﻴﺘﺴﻴﻛ )
14،13 (.
ﻚﻳﺮﺤﺗ رد نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺖﻴﻤﻫا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ تﺎﻌﻟﺎﻄﻣ رد ناﺪﻤﺨﺗ يرورﺎﺑ
ﻧژ و ﺘ داﺪﻌﺗ ﺶﻳاﺰﻓا و ﻚﻴ
ﻚﻤﺨﺗ ﺎﻫ زﺎﻴﻧ درﻮﻣ ي
، و ﻪﺟﻮﺗ ﺎﺑ ﻪﺑ دﻮﺒﻤﻛ رد ﻲﻤﻠﻋ تﺎﻌﻟﺎﻄﻣ
ﻂﻳاﺮﺷ نورد
5ﻲﻨﺗ ﻦﻴﭘوﺮﺗودﺎﻨﮔ درﻮﻣ رد ﺎﻫ
ﺑ ﻪ ﻊﻧاﻮﻣ ﺖﻠﻋ
ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺮﺛا ﺮﺿﺎﺣ ﺶﻫوﮋﭘ رد ،ﻲﻗﻼﺧا و ﻲﻧﻮﻧﺎﻗ ﺖﻈﻠﻏ ﺎﺑ نﺎﺴﻧا IU/ml
5 لﻮﻜﻴﻟﻮﻓ ﺮﺑ ﺎﻫ
ﻲﻧاﺪﻤﺨﺗ ي
شﻮﻣ ﺎﻫ ﻎﻟﺎﺑﺎﻧ ي Balb/c ﺪﺷ ﻲﺳرﺮﺑ
.
شور ﻖﻴﻘﺤﺗ
ا زا ﻖﻴﻘﺤﺗ ﻦﻳ تﺪﻣ ﻪﺑ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﻲﺑﺮﺠﺗ عﻮﻧ
12 هﺎﻣ
ﻲﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﻲﻨﻳﻮﻜﺗ ﻲﻟﻮﻠﺳ ﻲﺗﺎﻘﻴﻘﺤﺗ هﺎﮕﺸﻳﺎﻣزآ رد هﺎﮕﺸﻧاد ﻪﻳﺮﺼﺘﻨﻣ يرورﺎﺑﺎﻧ ﻲﻧﺎﻣرد ﻲﺗﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ و ﺪﻬﺸﻣ ﺪﺷ مﺎﺠﻧا ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ .
تاﺮﺛا ﻲﺳرﺮﺑ ياﺮﺑ
لﻮﻜﻴﻟﻮﻓ ﺮﺑ نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺎﻫ
زا ،ﻲﻧاﺪﻤﺨﺗ ي
شﻮﻣ ﺎﻫ ةﺮﻛﺎﺑ هدﺎﻣ ي 21
داﮋﻧ ةزور Balb/c
ﻲﺒﻳﺮﻘﺗ نزو ﺎﺑ
12 - 15 ﺪﺷ هدﺎﻔﺘﺳا مﺮﮔ .
شﻮﻣ ﺎﻫ ﻣ زا ﻪﻌﻟﺎﻄﻣ درﻮﻣ ي ﺆ
ﻪﺴﺳ
مﺮﺳ و ﻦﺴﻛاو ﻪﻴﻬﺗ ﺪﻬﺸﻣ يزار يزﺎﺳ
؛ﺪﺷ شﻮﻣ زا ﺎﻫ رد قﺎﺗا
يﺎﻣد ﺎﺑ تﺎﻧاﻮﻴﺣ يراﺪﻬﮕﻧ
±2 22 هرود و داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد 12
و ﻲﻳﺎﻨﺷور ﺖﻋﺎﺳ 12
ﺪﺷ يراﺪﻬﮕﻧ ﻲﻜﻳرﺎﺗ ﺖﻋﺎﺳ .
مﺎﻤﺗ رد
ﺪﻣ ،يراﺪﻬﮕﻧ ت ﺘﺷاد ﻲﺳﺮﺘﺳد اﺬﻏ و بآ ﻪﺑ تﺎﻧاﻮﻴﺣ
؛ﺪﻨ ﻏ ياﺬ
بآ زا و ﻪﻴﻬﺗ هدﺎﻣآ ترﻮﺻ ﻪﺑ نﺎﺳاﺮﺧ ﻪﻧاﻮﺟ ﺖﻛﺮﺷ زا ﺎﻬﻧآ ﻪﻴﻔﺼﺗ هدﺎﻔﺘﺳا هﺪﺷ ﻲﻣ
ﺪﻧدﺮﻛ . مﺎﺠﻧا ياﺮﺑ
،ﻪﻌﻟﺎﻄﻣ داﺪﻌﺗ 30
يوﺎﺴﻣ هوﺮﮔ ﻪﺳ رد ﻲﻓدﺎﺼﺗ ترﻮﺻ ﻪﺑ هﺮﻛﺎﺑ ةدﺎﻣ شﻮﻣ ﺮﺳ ﺪﻧﺪﺷ ﻢﻴﺴﻘﺗ ﻞﻳذ ﻖﺑﺎﻄﻣ :
- هوﺮﮔ ﺪﻫﺎﺷ : زا شﻮﻣ ﺎﻫ رد هوﺮﮔ ﻦﻳا ي شروﺮﭘ قﺎﺗا
In-vivo٥
ﺪﺷ يراﺪﻬﮕﻧ ﻲﻌﻴﺒﻃ ﻂﻳاﺮﺷ رد و تﺎﻧاﻮﻴﺣ .
- ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ هوﺮﮔ Sham-Exposed)
( : ﻪﺑ
شﻮﻣ ﺎﻫ هوﺮﮔ ﻦﻳا ي 1
/ 0 ﻲﻠﻴﻣ ﻦﻴﻟﺎﺳ لﺎﻣﺮﻧ لﻮﻠﺤﻣ ﺮﺘﻴﻟ 9
%
ﺪﺷ ﻖﻳرﺰﺗ .
- ﻲﺑﺮﺠﺗ هوﺮﮔ :
شﻮﻣ ﻪﺑ ﺎﻫ
هوﺮﮔ ﻦﻳا ي 1
/ 0 ﻲﻠﻴﻣ ﺮﺘﻴﻟ
نﻮﻣرﻮﻫ )hCG
IU/ml 5
1( ﺗ ﺪﻳدﺮﮔ ﻖﻳرﺰ )
15،11 .(
شﻮﻣ ﺎﻤﻴﺗ يﺎﻫ شﻮﻣ ﻦﻴﻨﭽﻤﻫ و هﺪﺷر
ﺎﻫ و ﺪﻫﺎﺷ هوﺮﮔ ي
ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ
، 18 ﻖﻳرﺰﺗ زا ﺲﭘ ﺖﻋﺎﺳ مﺮﻓوﺮﻠﻛ ﻂﺳﻮﺗ
ﻪﺘﺸﻛ ﺪﻧﺪﺷ . ﺢﻳﺮﺸﺗ زا ﺲﭘ
، ناﺪﻤﺨﺗ ﺎﻫ ي شﻮﻣ ﺎﻫ ﻪﺑ و جرﺎﺧ
ﺪﻳدﺮﮔ ﻞﻘﺘﻨﻣ يژﻮﻟﻮﻳﺰﻴﻓ مﺮﺳ يﻮﺘﺤﻣ فﺮﻇ .
فﺬﺣ زا ﺲﭘ
ﻲﺑﺮﭼ ﺎﻫ ناﺪﻤﺨﺗ فاﺮﻃا ﻲﻓﺎﺿا ي
، ﻂﺳﻮﺗ ﺎﻬﻧآ دﺎﻌﺑا
جرﺪﻣ پﻮﻜﺳوﺮﻜﻴﻣﻮﺋﺮﺘﺳا نزو و 2
يوزاﺮﺗ ﻂﺳﻮﺗ ﺎﻬﻧآ
ﻲﻟﺎﺘﻴﺠﻳد هزاﺪﻧا3
ﺖﺒﺛ و يﺮﻴﮔ ﺷ
ﺪ
؛ تﺎﻌﻟﺎﻄﻣ مﺎﺠﻧا ياﺮﺑ ﺲﭙﺳ
ﺖﻓﺎﺑ ﻲﺳﺎﻨﺷ زا هدﺎﻔﺘﺳا ﺎﺑ ناﺪﻤﺨﺗ ،يرﻮﻧ پﻮﻜﺳوﺮﻜﻴﻣ
ﺎﻫ
بآ زا ﺲﭘ و ﺖﻴﺒﺜﺗ ﺐﻟﺎﻗ و يﺮﻴﮔ
يﺮﻴﮔ
، شﺮﺑ ﺎﻫ ي ﻲﻟاﻮﺘﻣ
6 ﺲﻳرﺎﻫ ﻦﻳزﻮﺋا ﻦﻴﻠﻴﺴﻛﻮﺗﺎﻤﻫ شور ﻪﺑ و ﻪﻴﻬﺗ ﻲﻧوﺮﻜﻴﻣ
ﻧر ﮓ مﻻ و يﺰﻴﻣآ ﺎﻫ
ﺮﮔ ﻪﻴﻬﺗ ﻲﻤﺋاد ي ﺪﻳد
. لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ ﺎﻫ
ي
رد ،ﻪﻳﻮﻧﺎﺛ ،ﻪﻴﻟوا و ﺪﺷر لﺎﺣ
ﺰﻴﻧ لﻮﻜﻴﻟﻮﻓ ﺎﻫ رد ﻪﻴﻟوا لاﺮﺘﻧآ ي
ﺪﻳدﺮﮔ شرﺎﻤﺷ و ﻲﺳرﺮﺑ ﻲﻧﺎﻴﻣ ﻲﻤﻬﺳ ﻊﻃﺎﻘﻣ .
يﺮﻴﮔﻮﻠﺟ ياﺮﺑ
و بﺎﺨﺘﻧا لﻮﻜﻴﻟﻮﻓ ﻚﻳ اﺪﺘﺑا ،شرﺎﻤﺷ رد هﺎﺒﺘﺷا ﻪﻧﻮﮔﺮﻫ زا لﻮﻜﻴﻟﻮﻓ ﺮﻳﺎﺳ ﺎﻫ
ﻪﺑﺮﻘﻋ ﺖﻬﺟ رد ﺎﻫ
ﺪﻧﺪﺷ شرﺎﻤﺷ ﺖﻋﺎﺳ ي .
مﺎﻤﺗ ﺮﻣ ﻞﺣا ياﺮﺑ قﻮﻓ ﻪﻧﻮﻤﻧ
ﺎﻫ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ و ﺪﻫﺎﺷ ي
ﺪﺷ مﺎﺠﻧا ﺰﻴﻧ .
هداد ﺎﻫ
ﻤﻛ ي هدﺎﻔﺘﺳا ﺎﺑ ﻞﺻﺎﺣ ﻲ نﻮﻣزآ زا
ANOVA ﻤﻫ و
ﭽ ﻦﻴﻨ رﻮﻈﻨﻣ ﻪﺑ ﻲﺳرﺮﺑ
عاﻮﻧا داﺪﻌﺗ ﻦﻴﮕﻧﺎﻴﻣ
لﻮﻜﻴﻟﻮﻓ ﺎﻫ ﺲﭘ ﺎﺑ نﻮﻣزآ Kolmogorov-Smirnov رد
ﺢﻄﺳ ﻲﻨﻌﻣ يراد 05 / P<0 مﺮﻧ ﻚﻤﻛ ﻪﺑ يرﺎﻣآ راﺰﻓا
SPSS
و ﻪﻳﺰﺠﺗ ﺪﻳدﺮﮔ ﻞﻴﻠﺤﺗ
.
ﺮﺠﻣ رد نﺎﻳ مﺎﻤﺗ ﭘ مﺎﺠﻧا ﻞﺣاﺮﻣ
ﺶﻫوﮋ
، يراﺪﻬﮕﻧ ﺮﻴﻈﻧ
ﺖﻳﺎﻋر ﻪﺑ ﺪﻬﻌﺘﻣ ناﻮﻴﺣ ﺢﻳﺮﺸﺗ و ندﻮﻤﻧ شﻮﻬﻴﺑ ،ناﻮﻴﺣ ﻲﻗﻼﺧا لﻮﺻا ﻲﻫﺎﮕﺸﻳﺎﻣزآ تﺎﻧاﻮﻴﺣ ﺎﺑ رﺎﻛ
ﺪﻧدﻮﺑ .
ﺖﺧﺎﺳ١
ﺖﻛﺮﺷ
Organon
،
Holland
ﺎﺻ ﺖﻛﺮﺷ ﺖﺧﺎﺳ٢
ناﺮﻳا ناﺮﻳا ،
ﺖﻛﺮﺷ ﺖﺧﺎﺳ٣ Sartorius
، Germany
ﻪﺘﻓﺎﻳ ﺎﻫ ﻪﺴﻳﺎﻘﻣ نزو ﻦﻴﮕﻧﺎﻴﻣ و
ناﺪﻤﺨﺗ هزاﺪﻧا ﻦﻴﮕﻧﺎﻴﻣ ﺰﻴﻧ و
لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ ﺎﻫ
و ﺪﺷر لﺎﺣ رد ،ﻪﻳﻮﻧﺎﺛ ،ﻪﻴﻟوا ي ﺰﻴﻧ
ﻮﻜﻴﻟﻮﻓ ل ﺎﻫ شﻮﻣ ﻲﻧاﺪﻤﺨﺗ ﻪﻴﻟوا لاﺮﺘﻧآ ي ﺎﻫ
هوﺮﮔ ي ﺪﻫﺎﺷ
ﺎﺑ
توﺎﻔﺗ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ ﻲﻨﻌﻣ
يراد داﺪﻧ نﺎﺸﻧ )
05 / P>0 .(
هوﺮﮔ ﻦﻴﺑ ﻪﻳﻮﻧﺎﺛ و ﻪﻴﻟوا لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ ،ناﺪﻤﺨﺗ هزاﺪﻧا ﻪﺴﻳﺎﻘﻣ ﻲﻨﻌﻣ توﺎﻔﺗ ﺰﻴﻧ ﺪﻫﺎﺷ هوﺮﮔ و ﻲﺑﺮﺠﺗ داﺪﻧ نﺎﺸﻧ ار يراد
) 05 / P>0 ( . ناﺪﻤﺨﺗ نزو ﻂﺳﻮﺘﻣ ﺎﻫ
اﺪﻌﺗ ﻦﻴﮕﻧﺎﻴﻣ ، د
لﻮﻜﻴﻟﻮﻓ ﺎﻫ لﻮﻜﻴﻟﻮﻓ و ﺪﺷر لﺎﺣ رد ي ﺎﻫ
رد ﻪﻴﻟوا لاﺮﺘﻧآ ي
ﻪﻧﻮﻤﻧ ﺎﻫ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧ ﻲﺑﺮﺠﺗ هوﺮﮔ ي
ﺶﻳاﺰﻓا ﻲﻨﻌﻣ
يراد داد نﺎﺸﻧ )
001 / P=0 ماﺪﻛ ﺮﻫ ياﺮﺑ (
) لوﺪﺟ 1 (.
ﺚﺤﺑ
ﻲﻃ رد لﻮﻜﻴﻟﻮﻓ ﻮﻤﻧ و ﺪﺷر
ﺎﻫ ناراﺪﻧﺎﺘﺴﭘ رد
، ﻞﻣاﻮﻋ ﺪﺷر
ﻦﻴﭘوﺮﺗودﺎﻨﮔ و ﺎﻫ
هﺪﻧﺮﻴﮔ هاﺮﻤﻫ ﻪﺑ ﺎﻬﻧآ يﺎﻫ
دﺎﺠﻳا ﻲﻣ ﺪﻧﻮﺷ
) 16 .(
ﺺﺨﺸﻣ هﺪﺷ
لﻮﻠﺳ رد ﻪﻛ ﺖﺳا ﺎﻫ
سﻮﻟﻮﻣﻮﻛ ي
ﻲﻟﻮﻜﻴﻟﻮﻓ
، ﺮﻴﺛﺄﺗ ﺖﺤﺗ ﺰﻛﻮﻠﮔ ﻢﺴﻴﻟﻮﺑﺎﺘﻣ ﻦﻴﭘوﺮﺗودﺎﻨﮔ
ﺎﻫ ﺶﻳاﺰﻓا
ﻪﺘﻓﺎﻳ
، لﻮﻜﻴﻟﻮﻓ ﺪﺷر ﻪﺑ ﺮﺠﻨﻣ ﺖﻳﺎﻬﻧ رد ﺎﻫ
يﺮﭘ ي و لاﺮﺘﻧآ
لاﺮﺘﻧآ ﻲﻣ ددﺮﮔ ) 17 (
؛ ،نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﻦﻴﻨﭽﻤﻫ
گر يﺮﻳﺬﭘذﻮﻔﻧ ﺶﻳاﺰﻓا ﺐﺒﺳ ﺎﻫ
ي داﻮﻣ لﺎﻘﺘﻧا و ناﺪﻤﺨﺗ
لﻮﻜﻴﻟﻮﻓ ﺪﺷر و سﻮﻟﻮﻣﻮﻛ شﺮﺘﺴﮔ ياﺮﺑ يروﺮﺿ ﻲﻣ
ددﺮﮔ
لﻮﻜﻴﻟﻮﻓ ﺪﺷر ﺐﺒﺳ عﻮﺿﻮﻣ ﻦﻳا ﻪﻛ ﺎﻫ
لﻮﻜﻴﻟﻮﻓ ﺶﻳاﺰﻓا و ﺎﻫ
ي
ﺪﺷر لﺎﺣ رد ﻲﻣ
دﻮﺷ ) 18 (
؛ نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﻦﻴﻨﭽﻤﻫ
ﻲﻃ رد ﺰﻛﻮﻠﮔ نﻮﻴﺳاﺪﻴﺴﻛا ﺶﻳاﺰﻓا ﺐﺒﺳ ﻲﻫﺎﮕﺸﻳﺎﻣزآ غﻮﻠﺑ
ﻚﻤﺨﺗ هﺪﺷ وﺎﮔ
، ﺖﻴﻓﺮﻇ ﺎﻫ و ﺪﺷر ي ﺶﻳاﺰﻓا ار نآ يﻮﻤﻧ
ﻲﻣ ﺪﻫد ) 19 .(
تﺎﻌﻟﺎﻄﻣ نﻮﻣرﻮﻫ ﻪﻛ ﺖﺳا هداد نﺎﺸﻧ ﻲﻠﺒﻗ
ﻲﻟﻮﻜﻴﻟﻮﻓ ﻪﻛﺮﺤﻣ ﺐﻴﻛﺮﺗﻮﻧ
rFSH )
4( نﻮﻣرﻮﻫ و
5HMG
ﺗﺄ لﻮﻜﻴﻟﻮﻓ ﻮﻤﻧ و ﺪﺷر ﻪﻴﻟوا ﻞﺣاﺮﻣ ﺮﺑ يﺮﻴﺛ ﺎﻫ
ﻲﻧاﺪﻤﺨﺗ ي
دراﺪﻧ شﻮﻣ
؛ ﻲﻟﺎﺣ رد HMG ﻪﻛ
ﺎﻬﺘﻧا ﻞﺣاﺮﻣ ﺮﺑ ﻳ
ﻦﻳﻮﻜﺗ ﻲ
لﻮﻜﻴﻟﻮﻓ ﺎﻫ ﻣ ﺆ هدﻮﺑ ﺮﺛ
، ﻚﻤﺨﺗ ناﺰﻴﻣ يراﺬﮔ
ار ﺶﻳاﺰﻓا ﻲﻣ ﻫد ﺪ
recombinant Follicle Stimulating Hormone٤
٥
Human Menopausal Hormone
) 20 .(
ﻲﻣ نﺎﺸﻧ ﻲﻠﻌﻓ ﻪﻌﻟﺎﻄﻣ ﺞﻳﺎﺘﻧ ﻪﻛ ﺪﻫد
ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ
نﺎﺴﻧا لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ ﺎﻫ
ي ﻲﻧاﺪﻤﺨﺗ و ﺪﺷر لﺎﺣ رد
لﻮﻜﻴﻟﻮﻓ ﺎﻫ ﻪﻴﻟوا لاﺮﺘﻧآ ي ار شﻮﻣ رد
ﺶﻳاﺰﻓا ﻲﻣ ﺪﻫد ﺎﺑ ﻪﻛ
ﻪﺘﻓﺎﻳ ﺎﻫ ﻘﻘﺤﻣ ي ﺎ ﻂﻳاﺮﺷ رد ﻪﻛ ﻪﻨﻴﻣز ﻦﻳا رد ﻲﻠﺒﻗ ن نوﺮﺑ
ﻲﻨﺗ
) ﻲﻫﺎﮕﺸﻳﺎﻣزآ (
ﺖﺳا هﺪﺷ مﺎﺠﻧا
، ﺸﻣ دراد ﺖﻬﺑﺎ
؛ زا ﻚﻳ رد ﻪﻠﻤﺟ
ﻪﻌﻟﺎﻄﻣ
، نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ هﺪﻧﺮﻴﮔ نﺎﻴﺑ يﺎﻘﻟا ﺚﻋﺎﺑ
رد نوﺮﺘﺳژوﺮﭘ لﻮﻜﻴﻟﻮﻓ
ﺎﻫ ﻂﻴﺤﻣ رد شﻮﻣ ناﺪﻤﺨﺗ ﻪﻳﻮﻧﺎﺛ ي
ﻎﻟﺎﺑﺎﻧ شﻮﻣ مﺮﺳ يوﺎﺣ ﺖﺸﻛ هﺪﺷ
و ﺪﺷر ﺐﺒﺳ ﻪﺠﻴﺘﻧ رد و
لﻮﻜﻴﻟﻮﻓ يﺎﻘﺑ ﺎﻫ
لﻮﻜﻴﻟﻮﻓ ﺶﻳاﺰﻓا و ﺎﻫ
لاﺮﺘﻧآ و ﺪﺷر لﺎﺣ رد ي
ﺖﺳا هﺪﻳدﺮﮔ )
21 (.
ﺎﺘﻧ ﺞﻳ ﻌﻟﺎﻄﻣ Svensson ﻪ نارﺎﻜﻤﻫ و
ﻪﻛ ﺖﺳا ﺐﻠﻄﻣ ﻦﻳا ﺮﮕﻧﺎﻴﺑ اﺮﺤﺻ شﻮﻣ ناﺪﻤﺨﺗ رد
ﻳ
،ﻲ
ناﻮﻨﻋ ﻪﺑ نژوﺮﺘﺳا ﻞﻣﺎﻋ
هدﻮﻤﻧ ﻞﻤﻋ ﺎﻘﺑ
، نژورﺪﻧآ ﺚﻋﺎﺑ ﺎﻫ
ﻪﻣﺎﻧﺮﺑ گﺮﻣ يﺎﻘﻟا ﻲﻟﻮﻠﺳ هﺪﺷ يﺰﻳر
ﻲﻣ ﻧﻮﺷ رﺎﻤﻴﺗ زا ﺲﭘ و ﺪ
شﻮﻣ ﺎﻫ اﺮﺤﺻ ي ﻲﻳ
،نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺎﺑ ﺶﻫﺎﻛ
ﺐﻳﺮﺨﺗ لﻮﻠﺳ رد DNA
ﺎﻫ ﻮﻧاﺮﮔ ي ﺪﺷر ﺶﻳاﺰﻓا و ازﻮﻟ
لﻮﻜﻴﻟﻮﻓ ﺎﻫ هﺪﻫﺎﺸﻣ ﻲﻣ
دﻮﺷ ) 22 .(
تﺎﻌﻟﺎﻄﻣ Borman
و
نارﺎﻜﻤﻫ داد نﺎﺸﻧ ﺰﻴﻧ
ﻪﻛ ﺖﺤﺗ نوﺮﺘﺳژوﺮﭘ ﻲﻌﺿﻮﻣ ﺪﻴﻟﻮﺗ
ﺐﻳﺮﺨﺗ مﺪﻋ و ﺮﻴﺜﻜﺗ ﺐﺒﺳ ،نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﺮﻴﺛﺄﺗ لﻮﻠﺳ شﻮﻣ ،نﺎﺴﻧا يازﻮﻟﻮﻧاﺮﮔ يﺎﻫ ﺎﻫ
اﺮﺤﺻ ي ﻳ
تﺎﻤﻳﺮﭘ و ﻲ ﺎﻫ
رد نوﺮﺑ ﻂﻳاﺮﺷ ﻲﻨﺗ
ﻲﻣ ﺘﻧ رد ﻪﻛ دﻮﺷ ﺶﻳاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ ﻪﺠﻴ
لﻮﻜﻴﻟﻮﻓ ﺎﻫ ﺪﺷر لﺎﺣرد ي ﻲﻣ
ددﺮﮔ ) 23 (
؛ ﻦﻴﻨﭽﻤﻫ ﻪﺘﻓﺎﻳ
يﺎﻫ
ﻖﻴﻘﺤﺗ زا ﻞﺻﺎﺣ Klotchkov
نارﺎﻜﻤﻫ و يﺎﻫﺰﺧ يور ﺮﺑ
هدﺎﻣ
، ﻪﻛ ﺖﺳا ﺐﻠﻄﻣ ﻦﻳا ﺮﮕﻧﺎﻴﺑ ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ زا هدﺎﻔﺘﺳا
لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ ﺶﻳاﺰﻓا ﺐﺒﺳ نﺎﺴﻧا ﺎﻫ
ةرود زا ﺶﻴﭘ ﻎﻟﺎﺑ ي
سوﺮﺘﺳا ةرود رد و سوﺮﺘﺳا ﻲﻣ
ﺷ دﻮ رﻮﻧﺎﺟ ﻦﻳا رد يرورﺎﺑ و
ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﻂﺳﻮﺗ ﻚﻳﺮﺤﺗ ﻪﺑ ﻲﻟﻮﻜﻴﻟﻮﻓ ﺦﺳﺎﭘ ﻪﺑ ﻪﺘﺴﺑاو نﺎﺴﻧا ﻲﺘﻔﺟ ﻲﻣ
ﺪﺷﺎﺑ ) 24 (.
ﻪﺠﻴﺘﻧ يﺮﻴﮔ
ﻪﺘﻓﺎﻳ سﺎﺳا ﺮﺑ ﻲﻣ ،ﻪﻌﻟﺎﻄﻣ ﻦﻳا يﺎﻫ
ﻪﻛ ﺖﺷاد نﺎﻋذا ناﻮﺗ
ﻠﻣﺎﻋ نﺎﺴﻧا ﻲﺘﻔﺟ ﻦﻴﭘوﺮﺗودﺎﻨﮔ ﻲ
ﻣ ﺆ ﺎﻬﺘﻧا ﻞﺣاﺮﻣ رد ﺮﺛ ﻳ
ﻲ
لﻮﻜﻴﻟﻮﻓ ﻦﻳﻮﻜﺗ ﺎﻫ
شﻮﻣ ﻲﻧاﺪﻤﺨﺗ ي ﺖﺳا
ا ﺐﺒﺳ و ﺶﻳاﺰﻓ
لﻮﻜﻴﻟﻮﻓ ناﺰﻴﻣ ﺎﻫ
ﻦﻴﻨﭽﻤﻫ و ﻪﻴﻟوا لاﺮﺘﻧآ و ﺪﺷر لﺎﺣ رد ي
داﮋﻧ هدﺎﻣ شﻮﻣ رد ناﺪﻤﺨﺗ نزو ﺶﻳاﺰﻓا Balb/c
ﻲﻣ دﻮﺷ .
ﺮﻜﺸﺗ و ﺮﻳﺪﻘﺗ
ﻮﻜﺗ ﻲﺗﺎﻘﻴﻘﺤﺗ هﺎﮕﺸﻳﺎﻣزآ مﺮﺘﺤﻣ نارﺎﻜﻤﻫ زا ﻳ
ﻦ رﻮﻧﺎﺟ ي
ز هوﺮﮔ ﻳ ﺖﺴ ﺳﺎﻨﺷ ﻲ ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﻲ
ﻧ و ﺪﻬﺸﻣ ﻴ
ﺰ
ﻪﻳﺮﺼﺘﻨﻣ يرورﺎﺑﺎﻧ ﻲﻧﺎﻣرد ﻲﺗﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ مﺮﺘﺤﻣ نارﺎﻜﻤﻫ ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻣ
دﻮﺷ .
لوﺪﺟ 1 - ﻪﺴﻳﺎﻘﻣ آ لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ و ناﺪﻤﺨﺗ هزاﺪﻧا و نزو ﻦﻴﮕﻧﺎﻴﻣ يرﺎﻣ
ﻪﻴﻟوا لاﺮﺘﻧآ و ﺪﺷر لﺎﺣ رد ،ﻪﻳﻮﻧﺎﺛ ،ﻪﻴﻟوا يﺎﻫ
هوﺮﮔ رد يﺎﻫ ﺪﻫﺎﺷ و ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ ، ﻲﺑﺮﺠﺗ
ﻣ ﺎﻫ ﺮﻴﻐﺘ
هوﺮﮔ ﺎﻫ ناﺪﻤﺨﺗ هزاﺪﻧا
) ﺮﺘﻤﻴﻠﻴﻣ ( ناﺪﻤﺨﺗ نزو
) مﺮﮔ ( لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ
ﻪﻴﻟوا لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ
ﻪﻳﻮﻧﺎﺛ لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ
ﺪﺷر لﺎﺣ رد
لﻮﻜﻴﻟﻮﻓ داﺪﻌﺗ ﻪﻴﻟوا لاﺮﺘﻧآ
ﺪﻫﺎﺷ 42
/
±1 405 / 3 001
±./
0064 / 0 28 /
±0 85 / 1 23 /
±0 7 / 1 63
/
±0 8 / 6 11
/
±0 4 / 1
ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﺪﻫﺎﺷ
135 /
±0 665 / 3 00036 /
±0 0047 / 0 170 /
±0 45 / 2 206 /
±0 3 / 2 172 /
±0 86 / 5 178 /
±0 2
رﺎﻤﻴﺗ 1487 HCG
/
±0 86 / 3 00044 /
±0 0072 / 0 223 /
±0 55 / 3 133 /
±0 35 / 3 284 /
±0 35 / 7 209 /
±0 65 / 5
ﻲﻨﻌﻣ ﺢﻄﺳ يراد
338
/ 0 001
/ 0 082
/ 0 082
/ 0 001
/ 0 001
/ 0
ﻊﺑﺎﻨﻣ :
1- Thomas FH, Walters KA, Tefler EE. How to make a good oocyte: an update on in-vitro models to study follicle regulation. Hum Reprod Update. 2003; 9(6): 541-55.
2- Wandji SA, Srsen V, Nathanielsz PW, Eppig JJ, Fortune JE. Initiation of growth of baboon primordial follicles in vitro. Hum Reprod. 1997; 12(9): 1993-2001.
3- Fortune JE. The early stage of follicular development: activation of primordial follicles and growth preantral follicles. Anim Reprod Sci. 2003; 78(3-4): 135-63.
4- Hirshfield AN. Development of follicles in the mammalian ovary. Int Rev Cytol. 1991; 124: 43-101.
5- Stenman UH, Tiitinen A, Alfthan H, Valmu L. The classification, functions and clinical use of different isoforms of HCG. Hum Reprod Update. 2006; 12(6): 769-84.
6- Baharara J, Moosavifar N, Jalali M, Adl F. The effect of HCG on in vitro maturation of human immature oocytes and rate of 8 cell embryos mediated by ICSI. Armaghan Danesh. 2008; 12(4): 55-63. [Persian]
7- Baharara J, Moosavifar N, Jalali M, Maghani M. Effect of human menopause gonadotropin and recombinant follicle stimulating hormones on in vitro maturation of human immature oocytes and rate of eight cell embryos mediated by intracytoplasmic sperm injection. Iranian Journal of Obstetrics, Gynecology and Infertility. 2008; 11(3): 43-50.
[Persian]
8- Chian RC, Buckett WM, Tulandi T, Tan SL. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod.
2000; 15(1): 165-70.
9- De los Reyes M, de Lange J, Miranda P, Palominos J, Barros C. Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes. Theriogenology. 2005; 64(1):
1-11.
10- Yang SH, Son WY, Yoon SH, Ko Y, Lim JH. Correlation between in vitro maturation and expression of LH receptor in cumulus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles. Hum Reprod. 2005;
20(8): 2097-103.
11- Li Z, Jiang Q, Rezaei Sabet M, Zhang Y, Ritchie TC, Engelhardt JF. Conditions for in vitro maturation and artificial activation of ferret oocytes. Biol Reprod. 2002; 66(5): 1380-86.
12- Lechniak D, Szczepankiewicz D, Kauss D, Szulc J, Szydłowski M. IVM media, oocyte diameter and donor genotype at RYR1 locus in relation to the incidence of porcine diploid oocytes after maturation in vitro.
Theriogenology. 2005; 64(1): 202-12.
13- Gomes MK, Vieira CS, Moura MD, Manetta LA, Leite SP, Reis RM, et al. Controlled ovarian stimulation with exclusive FSH followed by stimulation with hCG alone, FSH alone or hMG. Eur J Obstet Gynecol Reprod Biol. 2007;
130(1): 99-106.
14- Branigan EF, Estes A. Use of micro-dose human chorionic gonadotropin (hCG) after clomiphene citrate (CC) to complete folliculogenesis in previous CC-resistant an ovulation. Am J Obstet Gynecol. 2005; 192(6): 1890-94.
15- Eppig JJ. Regulation of cumulus oophorus expansion by gonadotropins in vivo and in vitro. Biol Reprod. 1980;
23(3): 545-52.
16- McNatty KP, Heath DA, Lundy T, Fidler AE, Quirke L, O'Connell A, et al. Control of early ovarian follicular development. J Reprod Fertil Suppl. 1999; 54: 3-16.
17- Sutton ML, Cetica PD, Beconi MT, Kind KL, Gilchrist RB, Thompson JG. Influence of oocyte-secreted factors and culture duration on the metabolic activity of bovine cumulus cell complexes. Reproduction. 2003; 126(1): 27-34.
18- Nagyova E, Camaioni A, Prochazka R, Salustri A. Covalent transfer of heavy chains of inter-alpha-trypsin inhibitor family proteins to hyaluronan in in vivo and in vitro expanded porcine oocyte-cumulus complexes. Biol Reprod. 2004;
71(6): 1838-43.
19- Brackett BJ, Zuelke KA. Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology.
1993; 39(1): 43-64.
20- Baharara J, Moosavifar N, Jalali M, Moghani Lankarani M. Effects of HMG & R-FSH on development of ovarian follicles of female Balb/c mouse. Feyz. 2008; 12(1): 1-8. [Persian]
21- Rose UM, Hanssen RG, Kloosterboer HJ. Development and characterization of an in vitro ovulation model using mouse ovarian follicles. Biol Reprod. 1999; 61(2): 503-11.
22- Svensson EC, Markström E, Andersson M, Billig H. Progesterone receptor-mediated inhibition of apoptosis in granulosa cells isolated from rats treated with human chorionic gonadotropin. Biol Reprod. 2000; 63(5): 1457-64.
23- Borman SM, Chaffin CL, Schwinof KM, Stouffer RL, Zelinski-Wooten MB. Progesterone promotes oocyte maturation, but not ovulation, in nonhuman primate follicles without a gonadotropin surge. Biol Reprod. 2004; 71(1):
366-73.
24- Klotchkov DV, Eryuchenkov PA. Effects of hCG on folliculogenesis and fecundity in mink (Mustela vision Schreb). Theriogenology. 2003; 60(9): 1583-93.
Abstract Original Article
The effects of Human ChorionicGonadotropin (hCG) on development of ovarian follicles of Balb/c race mouse
J. Baharara1, N. Mosavifar2, M. Jalali2, F. Adl3
Background and Aim: Controlled ovarian stimulation (COS) using gonadotropin is an important technique in the infertility and genetics research. The effect of hCG on development of ovarian follicles of Balb/c race mouse was studied in this investigation.
Materials and Methods: In this experimental study, 30 virgin female Balb/c mice (21 days) were selected and divided into three groups of control, sham exposed (0.1ccNormal saline) and experimental group (0.1cc hCG 5IU/ml). The Mice were scarified 18 hours later by chloroform injection, and their ovaries were removed for morphometric and histological studies and the weight and size were recorded. The number of primary, secondary, growing and early antral follicles were determined and compared for each sample by light microscopy. The data were analyzed by means of SPSS software using ANOVA and Kolmogorov- Smirnov tests at the significant level of P<0.05.
Results: There was no significant difference in the size of ovary, the average number of primary and secondary follicle among the 3 groups of mice (P>0.05). The administration of hCG caused a significant increase in ovarian weight and the average number of growing and early antral follicles compared with control and sham exposed groups (P=0.001).
Conclusion: The results of this study indicate that the effect of hCG hormone on ovarian follicles growth rate, is higher in end-stages of development.
Key Words: Human chorionic gonadotropin (hCG), ovary, Balb/c mouse, follicular development
Journal of Birjand University of Medical Sciences. 2011; 17(4):274-280 Received: 18.08.2009 Last Revised: 05.10.2010 Accepted: 26.10.2010
1Corresponding Author; Assistant Professor, Department of Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran [email protected]
2 Assistant Professor, Montaserieh IVF Research Center, Mashhad University of Medical Science Mashhad , Iran
3 MSc of Cell & Developmental Biology, Department of Biology, Islamic Azad University Mashhad Branch, Mashhad ,Iran
