ﯽﺘﺴﯾز مﻮﻠﻋ ﯽﺷزرو
رﺎﻬﺑ–
13997–78: ص، 1ةرﺎﻤﺷ ،1 2ةرود 6 / 0 9 /05 : ﺖﻓﺎﯾرد ﺦﯾرﺎﺗ 98 /10 /17 : شﺮﯾﺬﭘ ﺦﯾرﺎﺗ 98
ﺮﯿﺛﺄﺗ ﺖﺸﻫ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ
ﻦ ﺑوﺎﻨﺗ ﯽ ﻣ ﺮﺑ ﯿ ناﺰ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ
ﺗوﺮﭘ ،ﻮﺗﻮﻠﮐ ﻟﻮﺌ
ﯿﭙ ﯿ ﺪ
ﺌﺗوﺮﭘ ﯿ ﻦ رﻮﻣﻮﺗزوﺮﮑﻧ رﻮﺘﮐﺎﻓ و ي
ﭽﺨﻣ ﺖﻓﺎﺑ رد ﺎﻔﻟآ ﮥ
شﻮﻣ يﺎﻫ ﻢﻟﺎﺳ
ﺪﻨﻤﻫﺮﻓ ﻪﻧﺎﺘﻓ –1
ﯽﻫﺎﺷرﻮﻧ ﻢﯾﺮﻣ
2∗
- ﯽﻧﺎﻤﯿﻠﺳ ﻢﯾﺮﻣ –3
ﯽﺒﺟر ﺪﯿﻤﺣ –4
روﺎﭘ ﻦﯾﻮﮐ
5
1.
ﻮﺠﺸ ﻧاد ي ﺮﺘﮐد ي ﻓ ﯿ ﺰﯾ ژﻮﻟﻮ ي ﺷزرو ﯽ ﺪﮑﺸ ﻧاد ة ﺷزرو مﻮﻠﻋ ﯽ
ﺘﺳرﺪﻨﺗ و ﯽ
، ﻬﺷ هﺎﮕﺸ ﻧاد ﯿ
ﺪ ﺘﺸ ﻬﺑ ﯽ ،
ناﺮﯾا ،ناﺮﻬﺗ 2
. ةﺪﮑﺸ ﻧاد رﺎﯿﺸ ﻧاد ﺷزرو مﻮﻠﻋ
ﯽ ﺘﺳرﺪﻨﺗ و ﯽ ، هﺎﮕﺸ ﻧاد ﻬﺷ ﯿ ﺪ ﯽﺘﺸ ﻬﺑ ، ناﺮﻬﺗ ناﺮﯾا ،
3.
دﺎﺘﺳا ﯾ رﺎ ﺸ ﻧاد هﺎﮕ ﺰﻬﺑ ﯾ ﺘﺴ ﯽ ﺸ ﺨﺒﻧاﻮﺗ و ﯽ
، ناﺮﯾا ،ناﺮﻬﺗ 4.
ﺸ ﻧاد ﯿ رﺎ ﺪﮑﺸ ﻧاد ة ﺑﺮﺗ ﯿ ﺖ ﻧﺪﺑ ﯽ ﺷزرو ﻢﻠﻋ و ﯽ
،
ﻣزراﻮﺧ هﺎﮕﺸ ﻧاد ﯽ
ناﺮﯾا ،ناﺮﻬﺗ ، 5
. دﺎﺘﺳا رﻮﻤﻣ هﺎﮕﺸ ﻧاد ﯾ
لﺎ ، ﺰﻧﺎﺟ ﺖﻨﺳ ، ادﺎﻧﺎﮐ
هﺪﯿﮑﭼ ﻘﺤﺗ رد هزوﺮﻣا ﯿ
تﺎﻘ ﺴﺑ ﯿ رﺎ ي هﺪﺷ هداد نﺎﺸﻧ ﺖﺳا
ﺮﻤﺗ ﻪﮐ ﯾ تﺎﻨ ﺷزرو ﯽ ﺮﺠﻨﻣ ﺰﻐﻣ دﺮﮑﻠﻤﻋ دﻮﺒﻬﺑ و ﺖﻣﻼﺳ ﻪﺑ ﯽﻣ
.دﻮﺷ
ﻨﭽﻤﻫ ﯿ ﻦ ﺴﻣ ﺖﺳا ﻦﮑﻤﻣ ﯿ
ﺎﻫﺮ ي ﺒﺼﻋ ﯽ ژﻮﻟورﻮﻧ و ﯾﮑ ﯽ ار رد ﺰﻐﻣ اﺰﻓا ﯾ ﺶ .ﺪﻨﻫد ﺮﯿﺛﺄﺗ ﯽﺳرﺮﺑ ﺮﺿﺎﺣ ﺶﻫوﮋﭘ زا فﺪﻫ 8
ﻪﺘﻔﻫ
ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ تﺪﺷ ﺎﺑ
80 ﺖﻋﺮﺳ ﺪﺻرد ﺎﺗ لوا ﮥﺘﻔﻫ رد ﻪﻨﯿﺸﯿﺑ
110 د ﻪﻨﯿﺸﯿﺑ ﺖﻋﺮﺳ ﺪﺻرد ﻮﺗﻮﻠﮐ ناﺰﯿﻣ ﺮﺑ ﺮﺧآ ﮥﺘﻔﻫ ر
،
وPLP TNF-α شﻮﻣ ﮥﭽﺨﻣ ﺖﻓﺎﺑ ﻦﯾﺪﺑ .دﻮﺑ ﻢﻟﺎﺳ يﺎﻫ
داﺪﻌﺗ ،رﻮﻈﻨﻣ 20
ﻪﺑ هدﺎﻣ شﻮﻣ ﺮﺳ ﻦﯾﺮﻤﺗ هوﺮﮔ ود ﻪﺑ ﯽﻓدﺎﺼﺗ رﻮﻃ
) 10
= (n لﺮﺘﻨﮐ و ) 10
= ( n ﻦﯾﺮﻤﺗ هوﺮﮔ رد .ﺪﻧﺪﺷ ﻢﯿﺴﻘﺗ
، تﺎﻧاﻮﯿﺣ 8 .ﺪﻧدﺮﮐ اﺮﺟا ار ﻞﯿﻣدﺮﺗ ﺮﺑ ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ ﻪﺑ اﺪﺘﺑا
تﺪﻣ 3 ﻗد ﯿ ﻪﻘ ﺖﻋﺮﺳ ﺎﺑ 6 ﺮﺘﻣ ﻗد ﺮﺑ ﯿ ﻪﻘ ود ﯾ ﺪ ﺪﻧ ﺮﻫ و 3 ﻪﻘﯿﻗد ﮏﯾ
،رﺎﺑ 3 ﻗد ﺮﺑ ﺮﺘﻣ ﯿ ﻪﻘ ﻪﺑ ﻣدﺮﺗ ﺖﻋﺮﺳ ﯿ
ﻞ ﻪﻓﺎﺿا ﺪﺷ ﺎﺗ
ﻧﺎﻣز ﯽ ا ﻆﻔﺣ ﻪﺑ ردﺎﻗ ﻪﮐ ﯾ
ﻦ ﺮﺧآ .ﺪﻨﺷﺎﺒﻧ تﺪﺷ ﯾ
ﻦ ﻪﺑ شﻮﻣ ﺮﻫ شﻼﺗ ﺎﻬﻧ رد .ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧرد ﺖﻋﺮﺳ ﺮﺜﮐاﺪﺣ ناﻮﻨﻋ
ﯾ ﺖ
،
ﺮﻤﺗ ﻞﮑﺗوﺮﭘ ﯾﻨ ﯽ تﺪﻣ ﻪﺑ 8 ﻪﺘﻔﻫ 5 .ﺪﺷ اﺮﺟا ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ ﻪﺑ تﻼﺑ نﺮﺘﺳو شور زا
هزاﺪﻧا رﻮﻈﻨﻣ ﻖﯿﻘﺤﺗ يﺎﻫﺮﯿﻐﺘﻣ يﺮﯿﮔ
هداد .ﺪﺷ هدﺎﻔﺘﺳا نﻮﻣزآ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫ
ﻞﻘﺘﺴﻣt ﺢﻄﺳ رد يرادﺎﻨﻌﻣ 05 / 0 P≤ ﻧﺪﺷ ﻞﯿﻠﺤﺗ ﻪﯾﺰﺠﺗ .ﺪ
نﺎﺸﻧ ﻖﯿﻘﺤﺗ ﺞﯾﺎﺘﻧ
داد
، ﻮﺗﻮﻠﮐ ناﺰﯿﻣ ﺶﯾاﺰﻓا ﻪﺑ ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ )
01 / 0 P≤ ( , )PLP 05 / 0 P≤ ( ناﺰﯿﻣ ﺶﻫﺎﮐ و TNF-α
) 05 / 0 P≤ ( رد هوﺮﮔ
.ﺪﺷ ﺮﺠﻨﻣ لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد ﻦﯾﺮﻤﺗ ﯽﻣ ﺖﯾﺎﻬﻧ رد
ﺖﻔﮔ ناﻮﺗ 8 ﻦﯿﺌﺗوﺮﭘ ناﺰﯿﻣ ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ يﺎﻫ
ﻦﯿﻠﯿﻣ زﺎﺳ
ناﺰﯿﻣ و ﺶﯾاﺰﻓا ار TNF-α
داد ﺶﻫﺎﮐ ار ﻪﺑ .
ﯽﻣ ﺮﻈﻧ ﺑ ﯽﺷزرو ﻦﯾﺮﻤﺗ ﺪﺳر ﻦﯿﻠﯿﻣ يﺎﻫﺮﮐرﺎﻣﻮﯿﺑ رد ﺶﯾاﺰﻓا ﺎ
زﺎﺳ
، ﯽﻤﻬﻣ ﺶﻘﻧ
رد .دراد يﺰﮐﺮﻣ بﺎﺼﻋا ﻢﺘﺴﯿﺳ زا ﺖﻇﺎﻔﺣ
هژاو يﺪﯿﻠﮐ يﺎﻫ
ﻟ ﻮﺌﺗوﺮﭘ ﯿﭙ ﯿ ﺪ ﺌﺗوﺮﭘ ﯿ ﻦ ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ، ،
،ﺎﻔﻟآ يرﻮﻣﻮﺗ زوﺮﮑﻧ رﻮﺘﮐﺎﻓ .ﻮﺗﻮﻠﮐ
: لﻮﺌﺴﻣ ةﺪﻨﺴﯾﻮﻧ∗
ﻦﻔﻠﺗ : 09126306358
nourshahi@sbu.ac.ir
- :b.m Email
ﻪﻣﺪﻘﻣ هﺪﺷ هداد نﺎﺸﻧ يرﺎﯿﺴﺑ تﺎﻘﯿﻘﺤﺗ رد هزوﺮﻣا ﺖﺳا
ﮑﻠﻤﻋ دﻮﺒﻬﺑ و ﺖﻣﻼﺳ ﻪﺑ ﯽﺷزرو تﺎﻨﯾﺮﻤﺗ ﻪﮐ ﺰﻐﻣ دﺮ
ﻣ ﺮﺠﻨﻣ ﯽ
؛دﻮﺷ د ار ﯽﮑﯾژﻮﻟورﻮﻧ و ﯽﺒﺼﻋ يﺎﻫﺮﯿﺴﻣ ﺖﺳا ﻦﮑﻤﻣ ﻦﯿﻨﭽﻤﻫ ر
ﺶﯾاﺰﻓا ﺰﻐﻣ .ﺪﻨﻫد
ﺣ ﺖﻣﻼﺳ ﻆﻔ
ﺑ زا يرﺎﯿﺴﺑ زا يﺮﯿﮕﺸﯿﭘ ياﺮﺑ ﺰﻐﻣ يرﺎﻤﯿ
يﺎﻫ ﺮﻣا يﺰﮐﺮﻣ بﺎﺼﻋا ﻢﺘﺴﯿﺳ ي
ﻪﺑ يروﺮﺿ ﻣ ﺮﻈﻧ
ﯽ ﺪﺳر ) 1 ( .
ﻦﯾاﺮﺑﺎﻨﺑ ، ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ ﯽﭘ رد
ﻋ ﻢﺘﺴﯿﺳ دﺮﮑﻠﻤﻋ ﺪﻨﻧاﻮﺘﺑ ﻪﮐ ﺪﻨﺘﺴﻫ ﯽﯾﺎﻫرﻮﺘﮐﺎﻓ ﯽﺧﺮﺑ ﻒﺸﮐ رد ار ﯽﺒﺼ
،ﻪﻨﯿﻣز ﻦﯾا رد ﺎﻫرﻮﺘﮐﺎﻓ ﻦﯾﺮﺗﺪﯾﺪﺟ زا ﯽﮑﯾ .ﺪﻨﻫد ﺶﯾاﺰﻓا داﺮﻓا ﻮﺗﻮﻠﮐ مﺎﻧ ﻪﺑ ﯽﻨﯿﺌﺗوﺮﭘ
ﺳا ﯽﻤﻬﻣ ﺶﻘﻧ ﻪﮐ ﺖ
ﻦﯿﻠﯿﻣ ﺖﻣﻼﺳ رد دراد
ﺮﺛﺆﻣ ﯽﻧﺎﻣرد ﺶﻘﻧ و ي
ﺑ زا يرﺎﯿﺴﺑ رد يرﺎﻤﯿ
يﺎﻫ ﮐﺮﻣ بﺎﺼﻋا ﻢﺘﺴﯿﺳ يﺰ
دراد
) 3،2 (
.
ﺌﺗوﺮﭘ ﯿ ﻦ ﻪﺑ ﻮﺗﻮﻠﮐ ﻣ نﺎﯿﺑ ﻪﯿﻠﮐ رد هﺪﻤﻋ رﻮﻃ ﯽ
،دﻮﺷ ﺑ ﺎﻣا د ﻮﺗﻮﻠﮐ نﺎﯿ ﯿﺣﺎﻧ ود ر
ﮥ مﺎﻧ ﻪﺑ ﺰﻐﻣ زا توﺎﻔﺘﻣ
لﻮﻠﺳ ﺎﻫ ي ﮑﺒﺷ لﺎﻤﯾﺪﻨﭘا ﮥ
ﺪﯿﺋوﺮﮐ ﻧ و1
ﻮ نور ﺎﻫ ي ﻪﺑ و ﺖﺳا ﺢﻄﺳ ﻦﯾﺮﺗﻻﺎﺑ رد ﻪﭽﺨﻣ ﮋﻨﮐرﻮﭘ ﻣ ﺮﻈﻧ
ﯽ ﺪﺳر رد
ﺮﺸﻗ و ﭗﻤﮐﻮﭙﯿﻫ ﺪﻨﻧﺎﻣ ﺰﻐﻣ ﯽﺣاﻮﻧ ﺮﯾﺎﺳ ﺰﻐﻣ
دراد دﻮﺟو
؛ .ﺖﺳا ﻢﮐ رﺎﯿﺴﺑ نآ نﺎﯿﺑ ﺎﻣا ﻮﺗﻮﻠﮐ
ﺑ ﯽ ﺶﻘﻧ ﮏﺷ
دﺮﮑﻠﻤﻋ رد ﯽﻤﻬﻣ رﺎﯿﺴﺑ ﻣ ﺎﻔﯾا ﻪﯿﻠﮐ
ﯽ ﺪﻨﮐ ﯽﺒﺼﻋ يﺎﻫدﺮﮑﻠﻤﻋ ﻦﯾا دﻮﺟو ﺎﺑ ، ﺰﯿﻧ ﻮﺗﻮﻠﮐ
ﻢﻬﻣ رﺎﯿﺴﺑ ﺪﻧا ) 5 ، 4 (
هداد نﺎﺸﻧ تﺎﻌﻟﺎﻄﻣ ﻼﺘﺒﻣ نارﺎﻤﯿﺑ رد ﻦﯿﻨﭽﻤﻫ و ﻦﺳ ﺶﯾاﺰﻓا ﺎﺑ ﻪﮐ ﺪﻧا
ﻪﺑ ﺮﻤﯾاﺰﻟآ و سا ما ،
ناﺰﯿﻣ نآ ﺶﻫﺎﮐ
ﻣ ﯽ ﺪﺑﺎﯾ ) 7 ، 6 (.
ﻪﺑ ﻦﯿﻨﭽﻤﻫ ﻣ ﺮﻈﻧ
ﯽ ﺪﺳر ﻮﺗﻮﻠﮐ نﺎﯿﺑ رﺎﻬﻣ ﻪﺑ ﺎﻔﻟآ يرﻮﻣﻮﺗ يزوﺮﮑﻧ رﻮﺘﮐﺎﻓ
رد ﺶﻫﺎﮐ و2
ﺖﯿﻟﺎﻌﻓ رﻮﺘﮐﺎﻓ ﻪﺘﺴﻫ ا ﺎﭘﺎﮐ ي ﻪﺑ3
ﺶﯿﭘ يﺎﻫرﻮﺘﮐﺎﻓ ناﻮﻨﻋ ﻣ ﺮﺠﻨﻣ ﯽﺒﺼﻋ ﻢﺘﺴﯿﺳ رد ﯽﺑﺎﻬﺘﻟا
ﯽ دﻮﺷ ) 9 ، 8 (.
نﺎﺸﻧ تﺎﻌﻟﺎﻄﻣ هداد
ﺪﻧا ﻮﺗﻮﻠﮐ لﻮﻠﺳ رد ﺎﻫ ي ﻟا ﺖﯿﺳورﺪﻧدﻮﮕﯿ ﻪﺑ
لﻮﻠﺳ ناﻮﻨﻋ ﺎﻫ
ي ﻣ ﯽﻠﺻا ﻦﯿﻠﯿ زﺎﺳ زا
ﺪﻧﺮﯿﮔ ﻪﺑ لﺎﺼﺗا ﻖﯾﺮﻃ ة
رد ًﻻﺎﻤﺘﺣا ﻪﮐ دﻮﺧ يزﺎﻨﯿﮐ ﻦﯾزوﺮﯿﺗ ﻟا
ﺖﯿﺳورﺪﻧدﻮﮕﯿ ﺎﻫ
دراد راﺮﻗ
، رﻮﻔﺴﻓ ﻪﺑ ﻪﻠﯾ
نﺪﺷ يﺎﻫﺮﯿﺴﻣ ﻦﯿﺌﺗوﺮﭘ
ﯽﺑ زﺎﻨﯿﮐ ﺮﺠﻨﻣ4
ﯽﻣ دﻮﺷ رد ﺶﯾاﺰﻓا ﺎﺑ ﺖﯾﺎﻬﻧ رد و ﺌﺗوﺮﭘ نﺎﯿﺑ
ﻦﯿ ﺪﯿﭙﯿﻟﻮﺌﺗوﺮﭘ يﺎﻫ
ﻦﯿﺌﺗوﺮﭘ
5 و ﻦﯿﺌﺗوﺮﭘ ﯽﺳﺎﺳا ﻣ
6ﻦﯿﻠﯿ ﻪﺑ ﺌﺗوﺮﭘ ناﻮﻨﻋ ﻦﯿ
يﺎﻫ ﻣ ﻦﯿﻠﯿ زﺎﺳ - دﻮﺑ ﺪﻫاﻮﺧ هاﺮﻤﻫ )
0 1
،1 1 (.
هﺎﺠﻨﭘ PLP
ﻣ ﺪﯿﻟﻮﺗ ﺖﯿﺳورﺪﻧدﻮﮑﯿﻟا ﻂﺳﻮﺗ ﻪﮐ ار ﻦﯿﻠﯿﻣ ﯽﻠﺻا ﻦﯿﺌﺗوﺮﭘ ﺪﺻرد ﯽ
،دﻮﺷ رد ﻢﺘﺴﯿﺳ
يﺰﮐﺮﻣ بﺎﺼﻋا ﻣ ﻞﯿﮑﺸﺗ
ﯽ ﺪﻫد ﻣ ﺎﻔﯾا ﻦﯿﻠﯿﻣ فﻼﻏ رد ﯽﻤﻬﻣ يرﺎﺘﺧﺎﺳ ﺶﻘﻧ و ﯽ
ﺪﻨﮐ رد ﻦﯿﺌﺗوﺮﭘ ﻦﯾا .
ﻪﺘﺴﺑ يﮋﻠﮔ هﺎﮕﺘﺳد ﻂﺳﻮﺗ و ﺰﺘﻨﺳ ﮏﯿﻤﺳﻼﭘﻮﮐرﺎﺳ مﻮﻠﮑﯿﺗر ﺪﻨﺑ
ي هﺪﺷ و ﯽﻃ رد ﺮﻓ ا ﯽﻟﻮﮑﯾزو لﺎﻘﺘﻧا ﺪﻨﯾ
1. Ependymal cells choroid plexus
2 . TNF-α
3. Nuclear Factor kappa-light-chain-enhancer of activated B cells 4. AKT
5. PLP 6. MBP
ﺮﯿﺛﺄﺗ ﺖﺸﻫ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ ﺑوﺎﻨﺗ ﯽ ﻣ ﺮﺑ ﯿ ناﺰ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ ﻟﻮﺌﺗوﺮﭘ ،ﻮﺗﻮﻠﮐ ﯿﭙ
ﯿ ﺪ ﺌﺗوﺮﭘ ﯿ ﻦ و . ..
69
ﺎﺸﻏ دراو ي ﻣ ﻦﯿﻠﯿﻣ ﯽ دﻮﺷ هداد نﺎﺸﻧ تﺎﻌﻟﺎﻄﻣ . ﻦﯿﺌﺗوﺮﭘ ﯽﮑﯿﺘﻧژ ﺺﻘﻧ ﻪﮐ ﺪﻧا
شﻮﻣPLP
ﺎﻫ ي ﯽﻫﺎﮕﺸﯾﺎﻣزآ
،
ﻣ ﺮﺠﻨﻣ ﯽﺘﮐﺮﺣ تﻻﻼﺘﺧا ﻪﺑ ﯽ
دﻮﺷ ﺎﺸﻏ رﺎﺘﺧﺎﺳ ﻆﻔﺣ رد ﯽﺗﺎﯿﺣ ﺶﻘﻧ ﻪﮐ ي
ﻦﯿﻠﯿﻣ دراد ) 13 ، 12 (.
رد صﻮﺼﺧ ﺮﯿﺛﺄﺗ ناﺰﯿﻣ ﺮﺑ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﻮﺗﻮﻠﮐ
يﺎﻫرﻮﺘﮐﺎﻓ و ﻣ ﻪﺑ طﻮﺑﺮﻣ
،ﻦﯿﻠﯿ ﯽﻤﮐ رﺎﯿﺴﺑ تﺎﻘﯿﻘﺤﺗ
مﺎﺠﻧا ﺘﻓﺮﮔ ﻪ ﺖﺳا ﻘﯿﻘﺤﺗ رد . ﯽ
يﺪﯿﻔﺘﺴﻣ رﺎﮑﻤﻫ و1
ا ن ) 2015 ( حﻮﻄﺳ ﻪﮐ ﺪﻧداد نﺎﺸﻧ ﻮﺗﻮﻠﮐ
داﺮﻓا رد
ﻦﯾﺮﻤﺗ دﺮﮐ ة لﺎﻌﻓﺮﯿﻏ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد ﻢﻟﺎﺳ ﺶﯾاﺰﻓا ﻖﯾﺮﻃ زا ﻢﻈﻨﻣ يزاﻮﻫ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ و ﺖﺳا ﺮﺘﺸﯿﺑ
رد
PGC1-α
ﻣ رد ﺶﯾاﺰﻓا ﻪﺑ ناﺰﯿ
ﻮﺗﻮﻠﮐ ﻣ ﺮﺠﻨﻣ نارﺎﮑﺷزرو ﻦﯾا رد ﯽ
دﻮﺷ ) 14 (
.
نﻮﮑﯿﻧ نارﺎﮑﻤﻫ و2
) 2018 ( ﻪﮐ ﺪﻧداد نﺎﺸﻧ ﮥﻣﺎﻧﺮﺑ هﺎﻣ ﻪﺳ
ﯽﻣواﺪﺗ و ﯽﺑوﺎﻨﺗ يزاﻮﻫ ﻦﯾﺮﻤﺗ رد
شﻮﻣ ﺎﻫ
زا ﺮﻃ ﯾ ﻖ ﺶﻫﺎﮐ ﻪﻧﻮﮔ ﺎﻫ ي ﺶﻨﮐاو ﺬﭘ نﮋﯿﺴﮐا ﺮﯾ
3، رد ﺶﯾاﺰﻓا ﻪﺑ ﻮﺗﻮﻠﮐ ناﺰﯿﻣ
ﺪﺷ ﺮﺠﻨﻣ ﻪﯿﻠﮐ و ﺰﻐﻣ ﺖﻓﺎﺑ رد
) 15 (
.
رد درﻮﻣ ﺮﺑ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﺮﺛا
، PLP
درﺎﻧﺮﺑ ز و4
نارﺎﮑﻤﻫ ) 2013 و 2016 ( ﺪﻧداد نﺎﺸﻧ 6
ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ
شﻮﻣ رد ﺎﻨﺷ ﺎﻫ
، ﺎﻘﻟا زا ﻞﺒﻗ ي ﻠﺣﺮﻣ زا يرﺎﻤﯿﺑ ﻢﺋﻼﻋ ﺶﻫﺎﮐ ﻪﺑ سا ما يرﺎﻤﯿﺑ ﮥ
ﺶﻫﺎﮐ ،يرﺎﻤﯿﺑ جوا ﺎﺗ عوﺮﺷ
α -
رد ﺶﯾاﺰﻓا ،TNF
ﺰﻐﻣ ﻖﺘﺸﻣ ﮏﯿﻓوﺮﺗوﺮﻧ رﻮﺘﮐﺎﻓ رد ﺶﯾاﺰﻓا و5
وPLP
ﻪﺑMBP
ﺌﺗوﺮﭘ ناﻮﻨﻋ ﻦﯿ
يﺎﻫ ﺮﯿﮔرد
ﻣ رد ﻦﯿﻠﯿ يزﺎﺳ ﺪﺷ ﺮﺠﻨﻣ ) 17 ، 16 (.
نﺎﻤﻫ ﺪﺷ حﺮﻄﻣ ﻪﮐرﻮﻃ ،
ﻮﺗﻮﻠﮐ ﻪﺑ ﻣ رد ﺮﺛﺆﻣ رﻮﺘﮐﺎﻓ ناﻮﻨﻋ ﻦﯿﻠﯿ
يزﺎﺳ
زا و دراد ﺶﻘﻧ آ
ﻪﺑ ﻪﮐ ﺎﺠﻧ ﻣ ﺮﻈﻧ ﯽ ﺪﺳر ﺛﺄﺗ ﺖﺤﺗ رﻮﺘﮐﺎﻓ ﻦﯾا ﺮﯿ
ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ دراد راﺮﻗ
، ﻖﻘﺤﻣ ﯽﭘ رد
ﻦﯾا ﻪﺑ ﯽﯾﻮﮕﺨﺳﺎﭘ ﺶﺳﺮﭘ
شﻮﻣ رد نﺪﯾود ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ﺎﯾآ ﻪﮐ ﺖﺳا ﻣ ﺎﻫ
ﯽ ﺪﻧاﻮﺗ ﺶﯾاﺰﻓا ﻪﺑ رد
ناﺰﯿﻣ ﻮﺗﻮﻠﮐ
ﺌﺗوﺮﭘ و ﻦﯿ يﺎﻫ ﻣ رد ﺮﯿﮔرد ﻦﯿﻠﯿ
يزﺎﺳ
؟دﻮﺷ ﺮﺠﻨﻣ
ﻖﯿﻘﺤﺗ شور ﻪﻧﻮﻤﻧ ﺎﻫ ﺶﻫوﮋﭘ ﻦﯾا رد ،ﺪﺷ اﺮﺟا ﯽﺑﺮﺠﺗ شور ﻪﺑ و يدﺎﯿﻨﺑ عﻮﻧ زا ﻪﮐ
20 دﺎﻣ شﻮﻣ ﺮﺳ ة
ﺶﺷ ﻪﺘﻔﻫ ا ي
C57BL/6
ﻦﯿﮕﻧﺎﯿﻣ ﺎﺑ 15
2± مﺮﮔ زا ﻮﺘﯿﺘﺴﻧا يزار جﺮﮐ يراﺪﯾﺮﺧ و رد ﯽﻄﯿﺤﻣ ﺎﺑ ﻦﯿﮕﻧﺎﯿﻣ يﺎﻣد 4 / 1 22± ﮥﺟرد
ﺘﻧﺎﺳ ﯽ داﺮﮔ و ﮥﺧﺮﭼ ﯽﯾﺎﻨﺷور - ﯽﮑﯾرﺎﺗ 12:12 رد ﺲﻔﻗ ﺎﻫ ي صﻮﺼﺨﻣ زا ﺲﻨﺟ ﻠﭘ ﯽ تﺎﻨﺑﺮﮐ يراﺪﻬﮕﻧ ﺪﻧﺪﺷ .
1. Mostafidi
2. Naichun 3. ROS 4. Bernards 5. BDNF
ﯽﻣﺎﻤﺗ تﺎﻧاﻮﯿﺣ ﻪﺑ بآ و ياﺬﻏ صﻮﺼﺨﻣ شﻮﻣ ﯽﺳﺮﺘﺳد دازآ ﺪﻨﺘﺷاد . ﻣﺎﻤﺗ و يراﺪﻬﮕﻧ ﻞﺣاﺮﻣ ﯽ ﺢﯾﺮﺸﺗ
شﻮﻣ سﺎﺳاﺮﺑ ﺎﻫ ﺮﻈﻧ
ﺘﯿﻤﮐ ﮥ مﺎﺠﻧا ﯽﺘﺸﻬﺑ ﺪﯿﻬﺷ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد قﻼﺧا ﺖﻓﺮﮔ
زا ﺲﭘ تﺎﻧاﻮﯿﺣ .
هﺎﮕﺸﯾﺎﻣزآ ﻂﯿﺤﻣ ﺎﺑ يزﺎﺳﺎﻨﺷآ
، ﻪﺑ ﻦﯾﺮﻤﺗ هوﺮﮔ ود ﻪﺑ ﯽﻓدﺎﺼﺗ رﻮﻃ )
EX, n=10
( لﺮﺘﻨﮐ هوﺮﮔ و
(Cont.,
n=10)
.ﺪﻧﺪﺷ ﻢﯿﺴﻘﺗ رد
هوﺮﮔ شﻮﻣEX
ﺎﻫ 8 ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ ﯽﺑوﺎﻨﺗ
تﺪﺷ ﺎﺑ 80 ﺖﻋﺮﺳ ﺪﺻرد ﺑ
رد ﻪﻨﯿﺸﯿ
ﺎﺗ لوا ﮥﺘﻔﻫ 110
ﺖﻋﺮﺳ ﺪﺻرد ﺑ
ﺮﺧآ ﮥﺘﻔﻫ رد ﻪﻨﯿﺸﯿ ﺪﻧدﺮﮐ اﺮﺟا ﻞﯿﻣدﺮﺗ ﺮﺑ ار
ﻞﮑﺷ) 1 (.
ﻞﮑﺷ 1 . زا ﮏﯿﺗﺎﻤﺷ حﺮﻃ هوﺮﮔ
يﺪﻨﺑ ﻖﯿﻘﺤﺗ
ﻦﯾﺮﻤﺗ ﻞﮑﺗوﺮﭘ ﻠﺣﺮﻣ رد اﺪﺘﺑا رد ﮥ
يزﺎﺳﺎﻨﺷآ
، شﻮﻣ تﺪﻣ ﻪﺑ و زور رد رﺎﺑ ود ﺎﻫ 5
زور ﺮﻫ و زور 10
ﺮﻔﺻ ﺐﯿﺷ ﺎﺑ ﻪﻘﯿﻗد
ﺖﻋﺮﺳ و 8 ﺘﻓر هار ﻞﯿﻣدﺮﺗ يور ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ ﺪﻨ
. ﺲﭘ رود نﺎﯾﺎﭘ زا ة
شﻮﻣ ،يزﺎﺳﺎﻨﺷآ اﺰﻓ ﺖﺴﺗ ﺎﻫ
ةﺪﻨﯾ
ﺮﯿﺛﺄﺗ ﺖﺸﻫ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ ﺑوﺎﻨﺗ ﯽ ﻣ ﺮﺑ ﯿ ناﺰ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ ﻟﻮﺌﺗوﺮﭘ ،ﻮﺗﻮﻠﮐ ﯿﭙ
ﯿ ﺪ ﺌﺗوﺮﭘ ﯿ ﻦ و . ..
71
دﺮﮐ اﺮﺟا ار ﯽﻧﺎﮑﻠﭘ ﺪﻧ
ﻪﺑ ﻪﮐ ﻪﺑ رﻮﻈﻨﻣ شﻮﻣ ﺖﻋﺮﺳ ﺮﺜﮐاﺪﺣ ندروآ ﺖﺳد تﺪﻣ ﻪﺑ اﺪﺘﺑا .ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧرد ﺎﻫ
3 ﺖﻋﺮﺳ ﺎﺑ ﻪﻘﯿﻗد 6
ﺮﺘﻣ ﺪﯾود ﻪﻘﯿﻗد ﺮﺑ ﺪﻧ
ﺮﻫ و 3 ﻪﻘﯿﻗد ﮏﯾ
،رﺎﺑ 3 ﻪﺑ ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ ﻪﻓﺎﺿا ﻞﯿﻣدﺮﺗ ﺖﻋﺮﺳ
ﺪﺷ ﯽﻧﺎﻣز ﺎﺗ ﻧ تﺪﺷ ﻦﯾا ﻆﻔﺣ ﻪﺑ ردﺎﻗ ﻪﮐ ﺪﻧدﻮﺒ
ﻪﺑ شﻮﻣ ﺮﻫ شﻼﺗ ﻦﯾﺮﺧآ . ﺮﻈﻧرد ﺖﻋﺮﺳ ﺮﺜﮐاﺪﺣ ناﻮﻨﻋ
،ﺖﯾﺎﻬﻧ رد .ﺪﺷ ﻪﺘﻓﺮﮔ تﺪﻣ ﻪﺑ ﯽﻨﯾﺮﻤﺗ ﻞﮑﺗوﺮﭘ
8 ﻪﺘﻔﻫ 5 تﺪﻣ ﻪﺑ اﺪﺘﺑا رد .ﺪﺷ اﺮﺟا ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ 5
تﺪﺷ ﺎﺑ ﻪﻘﯿﻗد 40
ﺎﺗ 50 ﻪﻨﯿﺸﯿﺑ ﺖﻋﺮﺳ ﺪﺻرد
، تﺪﺷ ﺎﺑ نآ زا ﺲﭘ و ندﺮﮐ مﺮﮔ ياﺮﺑ 50
- 40 ﺪﺻرد
ﺖﻋﺮﺳ ﺮﺜﮐاﺪﺣ
، ﺪﯾود نادﺮﮔ راﻮﻧ يور ندﺮﮐ دﺮﺳ ياﺮﺑ ﺪﻧ
) 19
، 18 ( لوﺪﺟ) 1 .(
لوﺪﺟ 1 . ﺮﻤﺗ ﻞﮑﺗوﺮﭘ ﯾ
ﻦ ﺑوﺎﻨﺗ ﯽ ﺪﺷ ﯾ ﺪ
تﻼﺑ نﺮﺘﺳو 48 ﮥﺴﻠﺟ ﻦﯾﺮﺧآ زا ﺲﭘ ﺖﻋﺎﺳ
،ﻦﯾﺮﻤﺗ ﻦﯾزﻮﻠﯾاز و ﻦﯿﻣﺎﺘﮐ زا ﯽﺒﯿﮐﺮﺗ ﺎﺑ تﺎﻧاﻮﯿﺣ ﺑ
ﯽ و ﺪﻧﺪﺷ شﻮﻫ
ﻪﺑ ﻪﭽﺨﻣ ﺖﻓﺎﺑ رﻮﻈﻨﻣ
هزاﺪﻧا ﮔ يﺮﯿ ﺌﺗوﺮﭘ ﻦﯿ يﺎﻫ .ﺪﺷ جرﺎﺧ ﻪﻃﻮﺑﺮﻣ ﻣآ زا ﺲﭘ
ﺎ ﻪﻧﻮﻤﻧ نﺪﺷ هد ﻦﯿﺌﺗوﺮﭘ ،ﺎﻫ
يﺎﻫ
ﻪﭽﺨﻣ ﺖﻓﺎﺑ رد دﻮﺟﻮﻣ
، ﻂﺳﻮﺗ
SDS-PAGE
زﺎﺳاﺪﺟ ي ﺪﺷ ه لﺎـﻘﺘـﻧا ﺎﺸﻏ ﻪﺑ و ﺪﺷ هداد
ﺘﻧآ ﺎـﺑ ﺎﺸــﻏ . ﯽ
يدﺎﺑ
ﯽﺻﺎﺼﺘﺧا )PLP
, USA Santa Cruz ,
58571 - Cat NO. sc
،(
) ﻮﺗﻮﻠﮐ
, Santa Cruz ,
22220 - Cat NO. sc
و (USA α -
)TNF , USA Santa Cruz ,
130349 - Cat NO. sc
( ﺎﺑ ﺎﺸﻏ تروﺎﺠﻣ زا ﺲﭘ و ﺪﺷ هداد تروﺎﺠﻣ
ﺘﻧآ ﯽ يدﺎﺑ يﺎﻫ ) ﻪﯾﻮﻧﺎﺛ
Cat NO. sc-516102 and sc-2020, Santa Cruz, USA
( راﺪﻧﺎﺸﻧ ﺪﺷ .
ﺖﻋﺮﺳ زور يروﺎﮑﯾر ﺖﻋﺮﺳ ﺮﺜﮐاﺪﺣ
)نﺪﯾود V max ( نﺎﻣز (min) ﻪﺘﻔﻫ
HIIT
9 5-1 ﺮﺑ ﺮﺘﻣ
ﻪﻘﯿﻗد 22
ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ 16
2interval-2min80%Vmax/ 1 40%Vmax
10 5-1 ﺮﺑ ﺮﺘﻣ
ﻪﻘﯿﻗد 24
ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ 24
4interval-2min90%Vmax/ 2 40%Vmax
11 5-1 ﺮﺑ ﺮﺘﻣ
ﻪﻘﯿﻗد 26
ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ 32
3،4،5 6interval-2min100%Vmax/
40%Vmax
9 5-1 ﺮﺑ ﺮﺘﻣ
ﻪﻘﯿﻗد 29
ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ 38
6،7،8 8interval-2min110%Vmax/
30%Vmax
ﺮﭘ ﺌﺗو ﻦﯿ يﺎﻫ ﮑﻔﺗ ﮏﯿ هﺪﺷ نﺎﯾﺮﺟ تﺪﺷ رد لژ يور ﺮﺑ 5
/ 0 تﺪﻣ ﻪﺑ ﺮﭙﻣآ 90
ﻪﻘﯿﻗد ﺰﻟﻮﻠﺳوﺮﺘﯿﻧ ﺬﻏﺎﮐ ﻪﺑ
،
ﻪﺑ .ﺪﺷ هداد لﺎﻘﺘﻧا بﻮﻃﺮﻣ و ﻪﺘﺳﻮﯿﭘ ترﻮﺻ ﺖﯾﺎﻬﻧ رد
، ﺘﻧآ لﻮﻠﺤﻣ رد ﺎﺸﻏ ﯽ
يدﺎﺑ تﺪﻣ ﻪﺑ ﺮﻓﺎﺑ رد ﻪﯾﻮﻧﺎﺛ 90
ﻪﻘﯿﻗد ﺎﺑ رﺎﺑ ود و ﺪﺷ هداد راﺮﻗ وTBST
ﮏﯾ رﺎﺑ ﺎﺑ ﺖﺴﺷPBS
لﻮﻠﺤﻣ ﯽﻓﺎﮐ راﺪﻘﻣ رد ﺎﺸﻏ .ﺖﻓﺮﮔ مﺎﺠﻧا ﻮﺷو
ياﺮﺘﺴﺑﻮﺳ ﯽﻧﺎﻣز ﺎﺗ ﺪﺷ هداد راﺮﻗTMB
ﺪﺷ ﻒﻗﻮﺘﻣ ﺮﻄﻘﻣ بآ ندﺮﮐ ﻪﻓﺎﺿا ﺎﺑ ﺶﻨﮐاو .ﺪﺷ ﺮﻫﺎﻇ ﺎﻫﺪﻧﺎﺑ ﻪﮐ
مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ،ﺎﻫﺪﻧﺎﺑ نﺪﺷ ﺮﻫﺎﻇ زا ﺲﭘ و راﺰﻓا
Image J
مﺎﺠﻧا ﯽﺑﺎﯾزرا .ﺖﻓﺮﮔ
ﺰﺠﺗ شور ﻪﯾ
ﻞﯿﻠﺤﺗو يرﺎﻣآ
ﺰﺠﺗ ﻪﯾ ﻞﯿﻠﺤﺗو هداد ﻫﺎ ي مﺮﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﻖﯿﻘﺤﺗ يرﺎﻣآ راﺰﻓا
Prism
ﺎﺑ
VER=5
مﺎﺠﻧا ﺖﻓﺮﮔ ﯽﻌﯿﺒﻃ .
هداد ندﻮﺑ ﻪﺑ ﺎﻫ ﺳو ﮥﻠﯿ فوﺮﮔﻮﻤﻟﻮﮐ -
حﻮﻄﺳ يرادﺎﻨﻌﻣ فﻼﺘﺧا ﯽﺳرﺮﺑ ياﺮﺑ .ﺪﺷ ﺺﺨﺸﻣ فﻮﻧﺮﯿﻤﺳا ﻮﺗﻮﻠﮐ
،
وPLP TNF- α
هوﺮﮔ رد ﺎﻫ ي ،ﻖﯿﻘﺤﺗ زا نﻮﻣزآ ﻞﻘﺘﺴﻣt
يرادﺎﻨﻌﻣ حﻮﻄﺳ .ﺪﺷ هدﺎﻔﺘﺳا 05
/ 0
P≤
ﺮﻈﻧرد
.ﺪﺷ ﻪﺘﻓﺮﮔ ﻪﺘﻓﺎﯾ و ﺞﯾﺎﺘﻧ ﻖﯿﻘﺤﺗ يﺎﻫ
ﺞﯾﺎﺘﻧ يرﺎﻣآ نﻮﻣزآ رد ﻞﻘﺘﺴﻣt
داد نﺎﺸﻧ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ
، 8 ﻪﺘﻔﻫ ،ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ﻪﺑ
ﻣ ﺶﯾاﺰﻓا ناﺰﯿ ﻮﺗﻮﻠﮐ
ﺑ هوﺮﮔ رد ﻪﭽﺨﻣ ﺖﻓﺎ ردEX
هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ لﺮﺘﻨﮐ
ﺠﻨﻣ ﺮ ﺪﺷ ) 01 /0 ) (P≤
رادﻮﻤﻧ .(2-B
ﺎﺘﻧ ﯾ ﺞ رﺎﻣآ نﻮﻣزآ ي
ﻞﻘﺘﺴﻣ t
رد داد نﺎﺸﻧ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ
، 8 ،ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ ﻪﺑ
ﻣ ﺶﯾاﺰﻓا ناﺰﯿ
ﻪﭽﺨﻣ ﺖﻓﺎﺑPLP
هوﺮﮔ رد ردEX
هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ لﺮﺘﻨﮐ
ﺪﺷ ﺮﺠﻨﻣ ) 5 /0 ( P≤
) رادﻮﻤﻧ .(2-C
ﺎﺘﻧ ﯾ ﺞ رﺎﻣآ نﻮﻣزآ ي
ﻞﻘﺘﺴﻣt
رد داد نﺎﺸﻧ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ
، 8 ،ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ ناﺰﯿﻣ ﺶﻫﺎﮐ ﻪﺑ
TNF-α
هوﺮﮔ رد ﻪﭽﺨﻣ ﺖﻓﺎﺑ ردEX
ﺠﻨﻣ لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ ﺮ
ﺪﺷ ) 1/
0
P≤
( ) رادﻮﻤﻧ .(2-D
ﺮﯿﺛﺄﺗ ﺖﺸﻫ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ ﺑوﺎﻨﺗ ﯽ ﻣ ﺮﺑ ﯿ ناﺰ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ ﻟﻮﺌﺗوﺮﭘ ،ﻮﺗﻮﻠﮐ ﯿﭙ
ﯿ ﺪ ﺌﺗوﺮﭘ ﯿ ﻦ و . ..
73
رادﻮﻤﻧ 1.
) ﻮﺗﻮﻠﮐ ناﺰﯿﻣ (B
PLP, ) (C TNF-α ) ( D .ﺖﻓﺮﮔ مﺎﺠﻧا تﻼﺑ نﺮﺘﺳو ﻂﺳﻮﺗ ﻪﭽﺨﻣ ﺖﻓﺎﺑ رد
و ﻮﺗﻮﻠﮐ ﺖﻈﻠﻏ ﻪﺑPLP
رﻮﻃ هوﺮﮔ رد يﺮﯿﮕﻤﺸﭼ ﺖﻈﻠﻏ .ﺖﻓﺎﯾ ﺶﯾاﺰﻓا لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ ردEX
TNF-α هوﺮﮔ رد ) ﺖﻓﺎﯾ ﺶﻫﺎﮐ لﺮﺘﻨﮐ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧEX
ﺮﯾدﺎﻘﻣ .(D ﻪﺑ ناﻮﻨﻋ ﻦﯿﮕﻧﺎﯿﻣ فاﺮﺤﻧا±
ﺪﺷ شراﺰﮔ دراﺪﻧﺎﺘﺳا )
05 /0 ** P≤ 01، /0 *P≤ (
ﺚﺤﺑ نﺎﺸﻧ تﺎﻌﻟﺎﻄﻣ هداد
ﺪﻧا ﯽﺷزرو تﺎﻨﯾﺮﻤﺗ ﺛﺄﺗ
ﯽﺘﺒﺜﻣ تاﺮﯿ ﺪﻧراد ﯽﺒﺼﻋ ﻢﺘﺴﯿﺳ دﺮﮑﻠﻤﻋ ﺮﺑ
ﯽﻔﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ .
ﻦﯿﺌﺗوﺮﭘ ﻢﻬﻣ ﺶﻘﻧ ﻮﺗﻮﻠﮐ
رد ار نآ دﺮﮑﻠﻤﻋ و ﻣ
ﻦﯿﻠﯿ ناﻮﻨﻋ يزﺎﺳ ﻣ
ﯽ ﻦﭼ .ﺪﻨﻨﮐ ) نارﺎﮑﻤﻫ و1
2013 ( نﺎﺸﻧ
ﺪﻧداد
، ﻮﺗﻮﻠﮐ ﺶﯾاﺰﻓا ﻪﺑ
ترﻮﺻ ﺰﻧژوﺰﮔا
، غﻮﻠﺑ و دﺮﮑﻠﻤﻋ رد ﺶﯾاﺰﻓا ﻪﺑ ﻟا
ﺖﯿﺳورﺪﻧدﻮﮕﯿ ﺪﻨﯾاﺮﻓ رد ﺶﯾاﺰﻓا و ﺎﻫ
ﻣ ﻦﯿﻠﯿ ﺮﺠﻨﻣ يزﺎﺳ ﻣ
ﯽ رد ﻮﺗﻮﻠﮐ نژ ندﺮﮐ توا كﺎﻧ ﻦﯿﻨﭽﻤﻫ .دﻮﺷ شﻮﻣ
ﺎﻫ نژ نﺎﯿﺑ ، ﺌﺗوﺮﭘ
ﻦﯿ ﺮﯿﮔرد يﺎﻫ
رد ﻣ ﻦﯿﻠﯿ ﺶﻫﺎﮐ ار يزﺎﺳ ﻣ
ﯽ ) ﺪﻫد 11 ﻦﯾاﺮﺑﺎﻨﺑ .(
، ﻮﺗﻮﻠﮐ ﻣ ﯽ ﺪﻧاﻮﺗ ﻪﺑ ناﻮﻨﻋ ﻼﺘﺒﻣ نارﺎﻤﯿﺑ ياﺮﺑ ﯽﻧﺎﻣرد فﺪﻫ
.دﺮﯿﮔ راﺮﻗ سا ما ﻪﺑ ﻘﺤﺗ رد
ﺮﺿﺎﺣ ﻖﯿ ﯽﺳرﺮﺑ ﻪﺑ
تاﺮﯿﺛﺄﺗ ﺌﺗوﺮﭘ ﺰﺘﻨﺳ ﺮﺑ ﯽﺷزرو ﻦﯾﺮﻤﺗ ﻦﯿ
يﺎﻫ رد ﺮﯿﮔرد
ﺪﻨﯾاﺮﻓ ﻣ ﻦﯿﻠﯿ يزﺎﺳ ﻪﭽﺨﻣ ﺖﻓﺎﺑ رد ﺞﯾﺎﺘﻧ .ﺪﺷ ﻪﺘﺧادﺮﭘ
ﻘﺤﺗ ﺮﺿﺎﺣ ﻖﯿ
،داد نﺎﺸﻧ 8
ﻪﺘﻔﻫ ﻪﺑ ،ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ
1. Chen C-D
ﺶﯾاﺰﻓا ناﺰﯿﻣ ،ﻮﺗﻮﻠﮐ وPLP
ﺶﻫﺎﮐ ناﺰﯿﻣ
TNF-α
وﺮﮔ رد ﻪﭽﺨﻣ ﺖﻓﺎﺑ رد ه
لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ ردEX
ﺮﺠﻨﻣ ﺪﺷ ) رادﻮﻤﻧ -
2-B,C,D
( .
رد صﻮﺼﺧ ﺮﺑ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﺮﺛا حﻮﻄﺳ
ﻮﺗﻮﻠﮐ ﺰﻐﻣ ﺖﻓﺎﺑ رد
، ﻪﺘﻓﺮﮔ مﺎﺠﻧا يدوﺪﺤﻣ رﺎﯿﺴﺑ تﺎﻌﻟﺎﻄﻣ
.ﺖﺳا ﻮﺴﻤﻫ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ ﺎﺑ
، نﻮﮑﯿﻧ نارﺎﮑﻤﻫ و )
2018 ( ﻪﺑ يزاﻮﻫ ﻦﯾﺮﻤﺗ هﺎﻣ ﻪﺳ ﺪﻧداد نﺎﺸﻧ رﻮﻃ
يﺮﯿﮕﻤﺸﭼ ﻣ نﺎﺸﻧ ﺞﯾﺎﺘﻧ ﻦﯾا .ﺪﺷ ﻮﺗﻮﻠﮐ نﺎﯿﺑ ﺶﯾاﺰﻓا ﺐﺟﻮﻣ
ﯽ ﺪﻫد ﺶﻫﺎﮐ ﻖﯾﺮﻃ زا يزاﻮﻫ ﻦﯾﺮﻤﺗ ﻪﮐ
ﻮﯿﺗاﺪﯿﺴﮐا سﺮﺘﺳا رد ﻮﺗﻮﻠﮐ نﺎﯿﺑ ،
ﺖﻓﺎﺑ ﻣ ﺶﯾاﺰﻓا ار ﻪﯿﻠﮐ و ﺰﻐﻣ ﯽ
ﺪﻫد ) 15 (.
ارﺎﺑﻮﺴﺗﺎﻣ نارﺎﮑﻤﻫ و1
) 2014
،(
ﺪﻧداد نﺎﺸﻧ 12
ﻪﺘﻔﻫ ﻦﯾﺮﻤﺗ ﻮﺗﻮﻠﮐ ﺢﻄﺳ ﺶﯾاﺰﻓا ،يزاﻮﻫ نﻮﺧ رد
نﺎﯾﺮﺷ ﯽﺘﻔﺳ ﺶﻫﺎﮐ و ﺮﺠﻨﻣ
ﻣ ﯽ دﻮﺷ .
ﻪﺑ ﻣ ﺮﻈﻧ ﯽ ﺪﺳر ﺪﻧﺮﯿﮔ ﺖﯿﻟﺎﻌﻓ و ﻮﯿﺗاﺪﯿﺴﮐا سﺮﺘﺳا ﺶﻫﺎﮐ ﻖﯾﺮﻃ زا ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ة
عﻮﻧ ﻦﯿﺴﻧﺎﺗﻮﯾﮋﻧآ ﻪﺑI
ﻣ ﺮﺠﻨﻣ ﻮﺗﻮﻠﮐ ﺶﯾاﺰﻓا ﯽ
دﻮﺷ ) 20 (.
ﻮﯿﻗﺎﺳ ) نارﺎﮑﻤﻫ و2
2016 ( ﺛﺄﺗ و ﻮﺗﻮﻠﮐ ناﺰﯿﻣ ﺮﺑ يزاﻮﻫ تﺎﻨﯾﺮﻤﺗ ﺮﯿ
- IGF
ﻮﺗﻮﻠﮐ حﻮﻄﺳ ﻪﮐ داد نﺎﺸﻧ ﻖﯿﻘﺤﺗ ﺞﯾﺎﺘﻧ .ﺪﻧدﺮﮐ ﯽﺳرﺮﺑ يﺮﻧوﺮﮐ قوﺮﻋ يرﺎﻤﯿﺑ ﻪﺑ ﻼﺘﺒﻣ نادﺮﻣ رد ار مﺮﺳ1
و
IGF-1
مﺮﺳ
، هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ رد ﻦﯾﺮﻤﺗ هوﺮﮔ رد دﻮﺑ ﺮﺗﻻﺎﺑ لﺮﺘﻨﮐ
) 21 ( ﺎﻄﻣ . مﺎﺠﻧا تﺎﻘﯿﻘﺤﺗ ﻖﺑ ﻪﺘﻓﺮﮔ
ﻣ ﯽ ناﻮﺗ ،ﺖﻔﮔ ﺎﻣ ﻖﯿﻘﺤﺗ رد
،
ًﻻﺎﻤﺘﺣا ﯽﺷزرو ﻦﯾﺮﻤﺗ ﺪﻧﺮﯿﮔ ﺖﯿﻟﺎﻌﻓ و ﻮﯿﺗاﺪﯿﺴﮐا سﺮﺘﺳا ﺶﻫﺎﮐ ﻖﯾﺮﻃ زا
ة
عﻮﻧ ﻦﯿﺴﻧﺎﺗﻮﯾﮋﻧآ ﺶﯾاﺰﻓا ،I
ﻦﯿﺌﺗوﺮﭘ زﺎﻨﯿﮐ لﺎﻌﻓ هﺪﺷ ﺎﺑ ﻦﯾزﻮﻧدآ تﺎﻔﺴﻓﻮﻧﻮﻣ
3 و α
- 1
رد ﺶﯾاﺰﻓا ﻪﺑPGC
ناﺰﯿﻣ ﻮﺗﻮﻠﮐ ﺮﺠﻨﻣ ﺖﺳا هﺪﺷ )
24 - 22 ، 20-14 (.
رد ناﺰﯿﻣ ﺮﺑ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﺮﺛا درﻮﻣ
، PLP
ﺞﯾﺎﺘﻧ ﺶﯾاﺰﻓا ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ ناﺰﯿﻣ
هوﺮﮔ رد ارPLP
ردEX
.داد نﺎﺸﻧ لﺮﺘﻨﮐ هوﺮﮔ ﺎﺑ ﻪﺴﯾﺎﻘﻣ يﺪﯿﻠﮐ ﻦﯿﺌﺗوﺮﭘ ﮏﯾPLP
و بﺎﺼﻋا ﻢﺘﺴﯿﺳ رد نﻮﯿﺳﺎﻨﯿﻠﯿﻣ لﻮﺌﺴﻣ
.ﺖﺳا يﺰﮐﺮﻣ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ ﺎﺑ ﻖﻓاﻮﻣ
، نﻮﯾ
4 و نارﺎﮑﻤﻫ ) 2016 ( نﺎﺸﻧ ﻦﯾﺮﻤﺗ ﻞﻣﺎﻌﺗ ﺪﻧداد يرﺎﯿﺘﺧا
نﺪﯾود
راﻮﻧ يور ﻪﺑ ناﺮﮔ تﺪﻣ 7 ﻪﺘﻔﻫ ﯽﺑﺮﭼ ﯽﯾاﺬﻏ ﻢﯾژر و
، ﺶﯾاﺰﻓا ﻪﺑ راﺪﻘﻣ
وPLP
ﻪﺑMBP
ناﻮﻨﻋ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ
يﺎﺸﻏ ﯽﻠﺻا ﻦﯿﻠﯿﻣ رد .ﺪﺷ ﺮﺠﻨﻣ عﺎﺨﻧ ﺪﻫاﻮﺷ
ﻪﺋارا هﺪﺷ ﯽﮐﺎﺣ زا نآ ﺖﺳا ﻪﮐ ﺮﺛا ﻞﺑﺎﻘﺘﻣ شزرو و ﯽﺑﺮﭼ
رد ﻢﯾژر ﯽﯾاﺬﻏ ﺐﺒﺳ نﻮﯿﺗﺮﯿﺳ ﺶﯾاﺰﻓا -
15
،
α 1 -
وPGC
ﯽﻣAKT
دﻮﺷ ﻪﮐ ﻦﮑﻤﻣ ﻪﺑ ﺶﯾاﺰﻓا ﻦﯿﻠﯿﻣ يزﺎﺳ
ﺮﺠﻨﻣ دﻮﺷ ) 25 ( . ﻤﻫ ﭽ ﻦﯿﻨ
Bernardes
) نارﺎﮑﻤﻫ و 2013
و 2016 ﺪﻧداد نﺎﺸﻧ ( 6
رد ﺎﻨﺷ ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ
شﻮﻣ ﺶﻫﺎﮐ ،يرﺎﻤﯿﺑ جوا ﺎﺗ عوﺮﺷ ﻪﻠﺣﺮﻣ زا يرﺎﻤﯿﺑ ﻢﺋﻼﻋ ﺶﻫﺎﮐ ﻪﺑ سا ما يرﺎﻤﯿﺑ يﺎﻘﻟا زا ﻞﺒﻗ ﺎﻫ
TNFα
،
1 . Matsubara
2 . Saghiv 3. AMPK 4. YOON 5. SIRT1
ﺮﯿﺛﺄﺗ ﺖﺸﻫ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ ﺑوﺎﻨﺗ ﯽ ﻣ ﺮﺑ ﯿ ناﺰ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ ﻟﻮﺌﺗوﺮﭘ ،ﻮﺗﻮﻠﮐ ﯿﭙ
ﯿ ﺪ ﺌﺗوﺮﭘ ﯿ ﻦ و . ..
75
رد ﺶﯾاﺰﻓا رد ﺶﯾاﺰﻓا وBDNF
وPLP
ﻪﺑMBP
ﻦﯿﺌﺗوﺮﭘ ناﻮﻨﻋ ﻦﯿﻠﯿﻣ رد ﺮﯿﮔرد يﺎﻫ
هﺪﺷ ﺮﺠﻨﻣ يزﺎﺳ
ﺖﺳا ) 17 ، 16 (.
ﺖﯾﺎﻬﻧ رد ﻣ ﯽ ناﻮﺗ زا ﯽﮑﯾ ،ﺖﻔﮔ يﺎﻫرﺎﮐوزﺎﺳ
ﺶﯾاﺰﻓا
PLP
ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ زا ﯽﺷﺎﻧ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ رد
،
ﺶﯾاﺰﻓا رد يﺎﻫﺮﯿﺴﻣ
،AKT SIRT1
و
PGC-1α
ﺖﺳا ﻪﮐ ﺶﯾاﺰﻓا ﺎﺑ د ر راﺪﻘﻣ ﺌﺗوﺮﭘ ﻦﯿ ﻪﺑ PLP
ناﻮﻨﻋ
ﺌﺗوﺮﭘ ﻦﯿ ﻣ ﻦﯿﻠﯿ زﺎﺳ دﻮﺑ ﺪﻫاﻮﺧ هاﺮﻤﻫ ، )
11 (.
ﻪﺑ ﯽﻠﺒﻗ تﺎﻌﻟﺎﻄﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻦﯿﻨﭽﻤﻫ ﺮﻈﻧ
ﻣ ﯽ ﺪﺳر
، لﺎﺼﺗا
رد دﻮﺧ ةﺪﻧﺮﯿﮔ ﻪﺑ ﻮﺗﻮﻠﮐ ﻟا
ﺖﯿﺳورﺪﻧدﻮﮕﯿ نﺪﺷ لﺎﻌﻓ و ﺎﻫ
ﺮﯿﺴﻣ ﺶﯾاﺰﻓا ﻪﺑ ،AKT
ﺌﺗوﺮﭘ ﻦﯿ ﺮﺠﻨﻣ PLP
ﻣ ﯽ ﺲﭘ .دﻮﺷ ﻣ
ﯽ ﺖﻔﮔ ناﻮﺗ ﺶﯾاﺰﻓا ﻞﯾﻻد زا ﯽﮑﯾ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ رد
ﺌﺗوﺮﭘ ﻦﯿ ،نآ ﯽﺘﺳدﻻﺎﺑ ﺮﯿﺴﻣ ،PLP
.ﺖﺳا هدﻮﺑ ﻮﺗﻮﻠﮐ ناﺰﯿﻣ رد ﺶﯾاﺰﻓا ﯽﻨﻌﯾ يﺎﻫرﻮﺘﮐﺎﻓ ﺮﺑ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﺮﺛا صﻮﺼﺧ رد ﭘ
ﺶﯿ ﯽﺑﺎﻬﺘﻟا
، ﻪﺑ ﯽﺑوﺎﻨﺗ ﻦﯾﺮﻤﺗ داد نﺎﺸﻧ ﺎﻣ ﻖﯿﻘﺤﺗ ﺞﯾﺎﺘﻧ
راﺪﻘﻣ ﺶﻫﺎﮐ
TNFα
ﻪﭽﺨﻣ ﺖﻓﺎﺑ رد ﺪﯿﻣﺎﺠﻧا
ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ ﺎﺑ ﻮﺴﻤﻫ .
، ﻌﻣ ﯿ ﻦ نارﺎﮑﻤﻫ و )
1396 ( ﻌﻟﺎﻄﻣ ﻪﺑ ﮥ
ﺛﺄﺗ ﺮﯿ 8 ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ زاﻮﻫ ي ﺌﺗوﺮﭘ ﺮﺑ ﯿ ﻦ ،ﻮﺗﻮﻠﮐ و IL-6
TNF-α
ﻠﮐ ﺖﻓﺎﺑ رد ﮥﯿ
شﻮﻣ ﺎﻫ ي اﺮﺤﺻ ﯽﯾ ﺮﻧ
ﺎﺘﻧ .ﺪﻨﺘﺧادﺮﭘ ﯾ
ﺞ ﻘﺤﺗ ﯿ ﻖ نﺎﺸﻧ ﺎﻬﻧآ داد
، ﺮﻤﺗ ﯾ ﻦ زاﻮﻫ ي اﺰﻓا ﺐﺟﻮﻣ ﯾ
ﺶ ﺌﺗوﺮﭘ ﯿ ﻦ ﻣ ﺶﻫﺎﮐ و ﻮﺗﻮﻠﮐ ﯿ
ناﺰ
IL-6
ﺤﺷﺮﺗ ﯽ ﻠﮐ ﺖﻓﺎﺑ ﯿ ﻪ ﯽﻣ اﺮﺑﺎﻨﺑ .دﻮﺷ ﯾ
ﻦ ﻣ ﯽ ناﻮﺗ ﺮﻤﺗ ًﻻﺎﻤﺘﺣا ﺖﻔﮔ ﯾ
ﻦ زاﻮﻫ ي ﺮﻃ زا ، ﯾ ﻖ اﺰﻓا ﯾ ﺶ ﻢﺋﻼﻋ ﻮﺗﻮﻠﮐ
ﻟﺎﺴﻨﻬﮐ ﯽ ﺧﺄﺗ ﻪﺑ ار ﺮﯿ ﯽﻣ دزاﺪﻧا ﺶﻨﮐاو و ﺎﻫ ي ﺑﺎﻬﺘﻟا ﯽ ﻣ ﺶﻫﺎﮐ ار ﯽ
ﺪﻫد ) 26 ( ﻄﻣ . زا ﯽﮑﯾ ﺪﻧداد نﺎﺸﻧ تﺎﻌﻟﺎ
ﺶﻘﻧ ﺎﻫ ﻮﺗﻮﻠﮐ .ﺖﺳا نآ ﯽﺑﺎﻬﺘﻟاﺪﺿ ﺶﻘﻧ ﻪﺑ ﻪﺟﻮﺗ ﻮﺗﻮﻠﮐ ي رﺎﻬﻣ ﻪﺑ
TNF-α
و
NF-Kβ
ﻪﺑ يﺎﻫرﻮﺘﮐﺎﻓ ناﻮﻨﻋ
ﻣ ﺮﺠﻨﻣ ﯽﺒﺼﻋ هﺎﮕﺘﺳد رد ﯽﺑﺎﻬﺘﻟا ﺶﯿﭘ ﯽ
دﻮﺷ ) 27 ، 8 (.
ياﺮﺑ تﺎﺒﺛا ﻦﯾا ﻪﺘﮑﻧ رد ﻖﯿﻘﺤﺗ
،ﺮﺿﺎﺣ ﻣ ناﺰﯿ
تاﺮﯿﯿﻐﺗ
TNF-α
هزاﺪﻧا ﮔ يﺮﯿ .ﺪﺷ ﺞﯾﺎﺘﻧ ﺮﺿﺎﺣ ﻖﯿﻘﺤﺗ نﺎﺸﻧ
اد د،
ﻣ راﺪﻘ
TNF-α
رد هوﺮﮔ رد EX
ﻪﺴﯾﺎﻘﻣ ﺎﺑ
هوﺮﮔ لﺮﺘﻨﮐ ﺮﺘﻤﮐ .دﻮﺑ ﻣ ﺲﭘ ﯽ ناﻮﺗ ﯽﮑﯾ ﺖﻔﮔ ﺮﮕﯾد
ﻞﯾﻻد زا ﺪﯾﺪﺟ ﺶﻫﺎﮐ
TNF-α
ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﯽﭘ رد
،
ناﺰﯿﻣ رد ﺶﯾاﺰﻓا ﺖﺳا هدﻮﺑ ﻮﺗﻮﻠﮐ
زا . فﺮﻃ
،ﺮﮕﯾد فﺮﺻ ﺮﻈﻧ زا ﺮﯿﺛﺄﺗ
،ﻮﺗﻮﻠﮐ ﺮﺛا ﺑﺎﻬﺘﻟاﺪﺿ ﯽ ةﻮﻘﻟﺎﺑ شزرو ﺮﺑ
ﺶﻫﺎﮐ ﺳ ﻦﯿﮐﻮﺘﯿ ﺖﺳا هﺪﺷ تﺎﺒﺛا يرﺎﯿﺴﺑ تﺎﻌﻟﺎﻄﻣ رد ﺎﻫ )
30-28 (
.
ﻪﺠﯿﺘﻧ ﮐ يﺮﯿﮔ ﯽﻠ
نﺎﻤﻫ ﻪﺑ ﻮﺗﻮﻠﮐ ،ﺪﺷ حﺮﻄﻣ ﻪﮐرﻮﻃ ﻦﯿﻠﯿﻣ ﺮﮐرﺎﻣﻮﯿﺑ ﮏﯾ ناﻮﻨﻋ
ﻪﺑ زﺎﺳ ﯽﻣ ﺮﻈﻧ ﺖﻣﻼﺳ رد ﯽﻤﻬﻣ ﻞﻣﺎﻋ ﺪﺳر
و يﺰﮐﺮﻣ بﺎﺼﻋا ﻢﺘﺴﯿﺳ يرﺎﻤﯿﺑ عﻮﻧ ﻦﯾا زا ﯽﺷﺎﻧ ﻢﺋﻼﻋ ﺶﻫﺎﮐ
ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﻪﺑ ﻪﺟﻮﺗ ﻦﯾاﺮﺑﺎﻨﺑ .ﺖﺳﺎﻫ
ﻪﺑ ﻮﺗﻮﻠﮐ ناﺰﯿﻣ ﺶﯾاﺰﻓا رد ﯽﯾورادﺮﯿﻏ ﻢﻬﻣ ﻞﻣﺎﻋ ﮏﯾ ناﻮﻨﻋ
، نﺎﺸﻧ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﺖﺒﺜﻣ ﺶﻘﻧ ةﺪﻨﻫد ياﺮﺑ
زا يﺮﯿﮕﺸﯿﭘ ﺑ
يرﺎﻤﯿ ﻪﺑ ﯽﺷزرو ﺖﯿﻟﺎﻌﻓ ﻦﯾاﺮﺑﺎﻨﺑ .ﺖﺳﺎﻫ ﻞﻣﺎﻋ ﮥﻟﺰﻨﻣ
ﺮﺛﺆﻣ رد ﺮﯿﮔرد يﺎﻫرﻮﺘﮐﺎﻓ ﺶﯾاﺰﻓا رد ي
ﻣ ﻦﯿﻠﯿ .دراد ﯽﻤﻬﻣ ﺶﻘﻧ داﺮﻓا رد ﻦﯿﻠﯿﻣ ﺐﯾﺮﺨﺗ ﺶﻫﺎﮐ و يزﺎﺳ
ﺬﺧﺂﻣ و ﻊﺑﺎﻨﻣ
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ﺮﯿﺛﺄﺗ ﺖﺸﻫ ﺮﻤﺗ ﻪﺘﻔﻫ ﯾ ﻦ ﺑوﺎﻨﺗ ﯽ ﻣ ﺮﺑ ﯿ ناﺰ ﻦﯿﺌﺗوﺮﭘ يﺎﻫ ﻟﻮﺌﺗوﺮﭘ ،ﻮﺗﻮﻠﮐ ﯿﭙ
ﯿ ﺪ ﺌﺗوﺮﭘ ﯿ ﻦ و . ..
79
The effect of 8 weeks of high intensity interval training on Klotho, PLP and TNF- α Cerebellum Tissue Female
Mice
Fataneh Farahmand1- Maryam Nourshahi∗2 - Maryam Soleimani3 -Hamid Rajabi4- Kouin Power5
1.Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran,
2. Department of Medical Basic Sciences, University of Social Welfare and Rehabilitation Sciences Tehran, Iran, 3 Department of Exercise Physiology, Sport Science Faculty, Kharazmi University, Tehran, Iran,
5. School of Human Kinetics and Recreation, Memorial University of Newfoundland, St. John’s, NL, Canada
(Received:2019/11/26;Accepted:2020/01/17)
Abstract
Nowadays, many studies have shown that exercise improves brain health and performance and may also increase neurological pathways in the brain. The aim of the present study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on Klotho, PLP and TNF-α concentrations in mice. Female mice were randomly divided to exercise (EX) or control (Con) groups. In the EX group, animals performed 8-week of HIIT. Mice initially ran at 6 m/min for 3 min at 0% grade, with the speed progressively increasing by 3 m/min every 3 min until exhaustion - when the mice were unable to maintain the required running speed. The maximal speed obtained was then used to calculate the individualized running speed for mice in the EX group. HIIT involved treadmill running 5 days per week for the final 6 weeks.
Klotho, PLP and TNF-α expressions were studied using Western blot. Mice in the EX group had a significant increase in klotho and PLP concentrations compared to the control group (p≤0.01 and p≤0.05, respectively). Also, in the exercise groups mice had a significant decrease in TNF-α concentrations in compared to the control group (p≤0.05). Our data demonstrate that HIIT increased klotho and PLP and deceased neuroinflammatory cytokines. These proteins are associated with increasing myelination and protect the central nerve system against neurodegenerative diseases.
Keyword
PLP, interval training, TNF-α, Klotho.
∗ Corresponding Author: Email: m-nourshahi@sbu.ac.ir ; Tel:+989126306358
