١٩
ﺮﻴﺛﺄﺗ ﻲﺳرﺮﺑ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ
ﺎﻫ اﺬﻏ داﻮﻣ زا هﺪﺷاﺪﺟ ي ﻳ
ﻲ ﺮﺑ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ
دﺎﺷاﺮﺧ يوﺮﺴﺧ ﺪﻤﺣا ﺮﺘﻛد -1
نﺎﻴﻗدﺎﺻ ﻲﻠﻋ ﺮﺘﻛد -2
مﺮﻛ ﻪﺟاﻮﺧ ﺰﻴﮕﻧا ﺮﻬﻣ ﺮﺘﻛد ﻦﻳﺪﻟا
-3
ﻲﻧﻼﻴﻣ ﻲﻀﺗﺮﻣ -4
ﻲﻠﻛﻮﺗ ﻪﻨﻴﻤﻬﺗ ﺮﺘﻛد 5
هﺪﻴﻜﭼ
ﻪﻨﻴﻣز فﺪﻫ و يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ :
ﻞﻣﺎﻋ رد ﺎﻬﺘﻧﻮﻔﻋ ﻦﻳﺮﺘﻌﻳﺎﺷ زا ﻲﻜﻳ ﺳﺎﻴﻧد
ﺖ
؛ ﻚﻴﺗﻮﻴﺑوﺮﭘ ﺎﻫ
ﻳ ﺪﻨﻧﺎﻣ ﻲ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
نژﻮﺗﺎﭘ زا ﻲﻌﻴﺳو ﻒﻴﻃ ﺪﺷر زا ﺎﻫ
يﺮﻴﮔﻮﻠﺟ ﻲﻧاﻮﻴﺣ و ﻲﻧﺎﺴﻧا ي ﻲﻣ
ﺪﻨﻨﻛ . ﻞﻴﺴﻧﺎﺘﭘ زا ﻲﻜﻳ ﺎﻫ
ﺪﻴﻔﻣ ي
ﻚﻴﺗﻮﻴﺑوﺮﭘ ﺎﻫ
ﺪﺿ ﺮﺑ ﺎﻬﻧآ ﻲﺘﺴﻴﻧﻮﮔﺎﺘﻧآ ﺮﺛا ﻢﺴﻴﻧﺎﮔراﺮﻜﻴﻣ
ﺎﻫ
ّﺮﻀﻣ ي ﻲﻣ ﺪﺷﺎﺑ
؛ ﺑ ﻪ ﻪﻛ يرﻮﻃ
ﻳا ﻢﺴﻴﻧﺎﮔرا ﻦ ﺎﻫ
رد
ﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ نﺎﻣرد و يﺮﻴﮕﺸﻴﭘ يرﻮﻠﻴ
ﻲﻣ ﺪﻨﺷﺎﺑ ﺪﻴﻔﻣ ﺪﻨﻧاﻮﺗ .
ﻦﻴﻴﻌﺗ فﺪﻫ ﺎﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﻲﮔﺪﻨﻨﻛرﺎﻬﻣ ﺶﻘﻧ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
اﺬﻏ داﻮﻣ رد دﻮﺟﻮﻣ ي ﻳ
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ يور ﻲ ﺪﺷ مﺎﺠﻧا
.
ﻖﻴﻘﺤﺗ شور ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد :
لﺎﺳ رد ﻪﻛ ﻲﻫﺎﮕﺸﻳﺎﻣزآ 1382
رد و نﺎﺘﺳرﺎﻤﻴﺑ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﺑ ﻪﺘﺴﺑاو ﻢﺋﺎﻗ
،ﺪﺷ مﺎﺠﻧا ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ ﺲﭘ
زا ﻲﺳرﺮﺑ و ﺖﺸﻛ 114
هﺪﻌﻣ ﻪﻧﻮﻤﻧ
، 20 ﺪﺷ اﺪﺟ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﻪﻧﻮﻤﻧ
؛
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
رد دﻮﺟﻮﻣ ي 12
ﻲﻨﺒﻟ هدﺎﻣ عﻮﻧ ﺰﻴﻧ
اﺪﺟ ﺪﻳدﺮﮔ ور ﻊﻳﺎﻣ و ﻳ ﺘﺷادﺮﺑ ﺎﻬﻧآ ﻲ ﺪﺷ ﻪ
ﺸﻛ زا ﺲﭘ و ﺖ
رﺎﮔآ ﺎﻴﺒﻤﻠﻛ ﻲﺻﺎﺼﺘﺧا ﺖﺸﻛ ﻂﻴﺤﻣ رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ
، ور ﻊﻳﺎﻣ ﺪﺷر رﺎﻬﻣ ﺮﺛا ﻳ
ﺖﺷادﺮﺑ ﻲ هﺪﺷ
، ﻮﻛﺬﻣ يﺮﺘﻛﺎﺑ ﺮﺑ ر
شور ﻪﺑ
Well Diffusion Agar
ﺪﺷ ﻲﺳرﺮﺑ .
ﻚﻳ ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ نﻮﻣزآ زا هدﺎﻔﺘﺳا ﺎﺑ تﺎﻋﻼﻃا ﺢﻄﺳ رد ﻪﻓﺮﻃ
ﻣ ﻲﻨﻌ يراد 05 /
P≤0 ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻞﻴﻠﺤﺗ درﻮﻣ ، .
ﻪﺘﻓﺎﻳ ﺎﻫ ﺮﻄﻗ ﻦﻴﮕﻧﺎﻴﻣ : ﺎﻫ
رد ﺪﺷر مﺪﻋ ﻪﻟ دراﻮﻣ مﺎﻤﺗ
1 /
±3 6 / 7 ﺮﺘﻤﻴﻠﻴﻣ دﻮﺑ ) 05 /
P<0 ( . ﺮﺘﺸﻴﺑ ﻪﻧﻮﻤﻧ ﺎﻫ ي
ﺗ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ور ﻊﻳﺎﻣ ﻂﺳﻮ
ﻳ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺖﺸﻛ زا ﻞﺻﺎﺣ ﻲ ﺎﻫ
ﺪﻧﺪﺷ رﺎﻬﻣ .
ﻚﻳ ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ نﻮﻣزآ رد ﻪﻓﺮﻃ
درﻮﻣ 12 ﻲﻨﻌﻣ ﻲﻨﺒﻟ ﻒﻠﺘﺨﻣ هدﺎﻣ دﻮﺑ راد
. ﺮﻄﻗ ﻦﻴﮕﻧﺎﻴﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ
هﺰﻳرﻮﺘﺳﺎﭘ ﺮﻴﻏ ﻲﻨﺒﻟ داﻮﻣ )
9 /
±2 2 / 8 (
، ﺮﺘﺸﻴﺑ
هﺰﻳرﻮﺘﺳﺎﭘ داﻮﻣ زا )
4 /
±3 5 / 7 ( دﻮﺑ ﻜﻴﻠﻫ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦﻴﮕﻧﺎﻴﻣ ﻦﻴﻨﭽﻤﻫ ؛ ﻲﻳور ﻊﻳﺎﻣ ﺮﺑاﺮﺑ رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮ
ﻲﻠﺤﻣ ﻲﻨﺒﻟ تﻻﻮﺼﺤﻣ زا هﺪﺷاﺪﺟ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ )
8 /
±1 9 / 8 ( ﻲﻠﺤﻣ ﺮﻴﻏ زا ﺮﺘﺸﻴﺑ )
6 /
±3 2 / 7 ( دﻮﺑ .
ﻪﺠﻴﺘﻧ يﺮﻴﮔ ﻲﻳاﺬﻏ داﻮﻣ ﻲﻳﺎﺳﺎﻨﺷ ،ﻚﻴﺗﻮﻴﺑوﺮﭘ يوﺎﺣ ﻲﻨﺒﻟ داﻮﻣ زا نوﺰﻓازور هدﺎﻔﺘﺳا ،ﻖﻴﻘﺤﺗ ﻦﻳا ﺞﻳﺎﺘﻧ سﺎﺳا ﺮﺑ:
ﻢﺴﻴﻧﺎﮔرا ﻲﻨﺒﻟ ﻲﻳاﺬﻏ داﻮﻣ ﻪﻴﻬﺗ ،ﺪﻴﻔﻣ راد ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻦﻳﺮﺗﺮﺛﺆﻣ و ﻦﻳﺮﺘﺸﻴﺑ يوﺎﺣ ﻪﻛ
و ﺪﻨﺷﺎﺑ ﺰﻴﻧ ﻪﻓﺎﺿا ندﺮﻛ
ﻲﻣ ﻪﻴﺻﻮﺗ ﻲﻨﺒﻟ داﻮﻣ رد ﺪﻴﻔﻣ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ دﻮﺷ
.
هژاو يﺪﻴﻠﻛ يﺎﻫ ؛يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ :
ﻚﻴﺗﻮﻴﺑوﺮﭘ ؛ﻞﻴﺳﺎﺑﻮﺘﻛﻻ
ﺪﻨﺟﺮﻴﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻤﻠﻋ ﻪﻠﺠﻣ )
هرود 13 هرﺎﻤﺷ ؛ 1 لﺎﺳ رﺎﻬﺑ ؛ 1385
(
ﺖﻓﺎﻳرد : 11 / 8 / 84 ﺎﻬﻧ حﻼﺻا
ﻲﻳ : 4 / 2 / 85 شﺮﻳﺬﭘ
: 14 / 2 / 85
؛لوﺆﺴﻣ هﺪﻨﺴﻳﻮﻧ1
ﮔ رﺎﻴﺸﻧاد هوﺮ ﻲﺷزﻮﻣآ ﻲﻠﺧاد - شراﻮﮔ
،ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ، ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
سردآ : ﺪﻬﺸﻣ - ﻢﺋﺎﻗ نﺎﺘﺳرﺎﻤﻴﺑ -
ﻲﻠﺧاد هوﺮﮔ ﻦﻔﻠﺗ
: 8407406 - 0511 ﺮﺑﺎﻤﻧ : 8407406 - 0511 ﻲﻜﻴﻧوﺮﺘﻜﻟا ﺖﺴﭘ [email protected]:
هوﺮﮔ رﺎﻴﺸﻧاد2
ﻲﺷزﻮﻣآ بوﺮﻜﻴﻣ ﻲﺳﺎﻨﺷ ،ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ، ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
ﺪﻬﺸﻣ
بوﺮﻜﻴﻣ هوﺮﮔ دﺎﺘﺳا3
ﻲﺳﺎﻨﺷ ،ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ، ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
بوﺮﻜﻴﻣ ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ يﻮﺠﺸﻧاد4
ﻲﺳﺎﻨﺷ
ﻲﺷزﻮﻣآ هوﺮﮔ ﻲﺼﺼﺨﺗ رﺎﻴﺘﺳد5
ﻲﻠﺧاد
٢٠ ﻪﻣﺪﻘﻣ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ
، رد ﺎﻬﺘﻧﻮﻔﻋ ﻦﻳﺮﺘﻌﻳﺎﺷ زا ﻲﻜﻳ ﻞﻣﺎﻋ
ﺖﺳﺎﻴﻧد
؛ ﺑ ﻪ ﻪﻛ يرﻮﻃ 70
- 90 % زا لﺎﺣ رد يﺎﻫرﻮﺸﻛ ﺖﻴﻌﻤﺟ
ﻪﻌﺳﻮﺗ
، زا ﻞﺒﻗ ﻦﺳ رد 10
ﻪﺑ ﻲﮕﻟﺎﺳ ﻳا
هدﻮﻟآ يﺮﺘﻛﺎﺑ ﻦ ﻲﻣ
ﺪﻧﻮﺷ
) 1 ( . ﺶﻳاﺰﻓا عﻮﻴﺷ ﺎﺑ ﻲﮔدﻮﻟآ ﻦﺘﻓرﻻﺎﺑ
ﻦﺳ ﺖﺳا هﺪﺷ شراﺰﮔ
) 2 ( . ﻮﻋ ﺪﻳﺎﺷ نﺎﺑﺰﻴﻣ ﻲﻜﻴﺘﻧژ و ﻲﻄﻴﺤﻣ ﻞﻣا
،
ﻤﻬﻣ ﺶﻘﻧ رد ﻲ
ﻲﮔدﻮﻟآ ﻪﺘﺷاد ﺪﻨﺷﺎﺑ ) 3 .(
ﻳا ﺎﺑ ﻲﻨﺷور طﺎﺒﺗرا ﻢﺴﻴﻧﺎﮔرا ﻦ
رﺎﻤﻴﺑ ﻳ ﭘﺮﺴﻟوا ،ﺖﻳﺮﺘﺳﺎﮔ يﺎﻬ ﭙ
مﻮﻔﻨﻟ و مﻮﻨﻴﺳرﺎﻛﻮﻧدآ ،ﻚﻴﺘ
(MALT) دراد هﺪﻌﻣ
) 4 - 8 ( .
رﻮﻠﻓ ﻦﻴﺑ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻞﺧاﺪﺗ ﻲﻌﻴﺒﻃ
ﻢﺴﻴﻧﺎﮔرا و ﺎﻫ
نژﻮﺗﺎﭘ ي
اوا رد ﻳ ﺑ و ﺪﺷ حﺮﻄﻣ ﻢﺘﺴﻴﺑ نﺮﻗ ﻞ ﻪ
ﺑ زا هدﺎﻔﺘﺳا نآ لﺎﺒﻧد ﻲﺧﺮ
يﺮﺘﻛﺎﺑ ﺎﻫ ﺖﻓﺮﮔ راﺮﻗ ﻪﺟﻮﺗ درﻮﻣ ﺎﻬﺘﻧﻮﻔﻋ لﺮﺘﻨﻛ رد
؛ ﺞﻳﺎﺘﻧ
مﺎﺠﻧا تﺎﻘﻴﻘﺤﺗ نﺎﺸﻧ هﺪﺷ
ﻲﻣ ﺪﻫد ﻪﻛ ﻚﻴﺗﻮﻴﺑوﺮﭘ ﺎﻫ
ﺪﻨﻧﺎﻣ ﻲﻳ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
نژﻮﺗﺎﭘ زا ﻲﻌﻴﺳو ﻒﻴﻃ ﺪﺷر زا ﺎﻫ
و ﻲﻧﺎﺴﻧا ي
يﺮﻴﮔﻮﻠﺟ ﻲﻧاﻮﻴﺣ ﻲﻣ
ﺪﻨﻨﻛ . ﻞﻴﺴﻧﺎﺘﭘ زا ﻲﻜﻳ ﺎﻫ
ﺪﻴﻔﻣ ي
ﻚﻴﺗﻮﻴﺑوﺮﭘ ﺎﻫ
، ﺘﻧآ ﺮﺛا ﻢﺴﻴﻧﺎﮔرا وﺮﻜﻴﻣ ﺪﺿ ﺮﺑ ﺎﻬﻧآ ﻲﺘﺴﻴﻧﻮﮔﺎ ﺎﻫ
ي
ّﺮﻀﻣ ﻲﻣ ﺪﺷﺎﺑ
؛ ﺑ ﻪ ﻪﻛ يرﻮﻃ ﻳا
ﻢﺴﻴﻧﺎﮔرا ﻦ ﺎﻫ
و يﺮﻴﮕﺸﻴﭘ رد
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ نﺎﻣرد ﻲﻣ
ﺷﺎﺑ ﺪﻴﻔﻣ ﺪﻨﻧاﻮﺗ ﻨ
ﺪ ) 10،9 (.
ﻲﺘﻧآ فﺮﺼﻣ ﻪﻛ ﺎﺠﻧآ زا ﻚﻴﺗﻮﻴﺑ
ﺎﻫ ﻪﺸﻳر ياﺮﺑ ﻦﻛ
ندﺮﻛ
ﻪﺘﺳاﻮﺧﺎﻧ ﻲﺒﻧﺎﺟ تاﺮﺛا يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ يا
ﺑ رﺎﻤﻴﺑ ياﺮﺑ ﻪ
لﺎﺒﻧد دراد ) 11 (
، ﻞﺜﻣ هﺪﻧز ﻲﺑوﺮﻜﻴﻣ ﻞﻣاﻮﻋ زا هدﺎﻔﺘﺳا
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
ﻲﻳاﺬﻏ داﻮﻣ ي )
ﻚﻴﺗﻮﻴﺑوﺮﭘ ﺎﻫ
( تاﺮﺛا ﺪﻗﺎﻓ ﻪﻛ
نﺎﻳز ﺖﺑﺎﻗر نﺪﺑ رد نژﻮﺗﺎﭘ ﻞﻣاﻮﻋ ﺎﺑ و ﺪﻨﺘﺴﻫ روآ ﻲﻣ
ﺪﻨﻨﻛ ﺎﻳ و
ﻦﻴﺑ زا ار ﺎﻬﻧآ ﻲﻣ
ﺪﻧﺮﺑ
، ﺖﺳا لﻮﺒﻗ ﻞﺑﺎﻗ و ﻲﻘﻄﻨﻣ .
ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﻦﻴﻴﻌﺗ فﺪﻫ ﺎﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ،قﻮﻓ ﺐﻟﺎﻄﻣ ﺶﻘﻧ
ﻲﮔﺪﻨﻨﻛرﺎﻬﻣ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ اﺬﻏ داﻮﻣ رد دﻮﺟﻮﻣ يﺎﻫ
ﻳ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ يور ﻲ
ﺪﺷ مﺎﺠﻧا .
ﻖﻴﻘﺤﺗ شور
ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد لﺎﺳ رد ﻪﻛ ﻲﻫﺎﮕﺸﻳﺎﻣزآ
1382 رد و
نﺎﺘﺳرﺎﻤﻴﺑ مﺎﺠﻧا ﺪﻬﺸﻣ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﺑ ﻪﺘﺴﺑاو ﻢﺋﺎﻗ
ﺪﺷ
، ﻲﺳرﺮﺑ و ﺖﺸﻛ زا ﺲﭘ 114
،هﺪﻌﻣ ﻪﻧﻮﻤﻧ 20
هدﻮﻟآ ﻪﻧﻮﻤﻧ نآ زا ﻪﻛ دﻮﺑ
ﺪﺷ اﺪﺟ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﻦﻴﻨﭽﻤﻫ ؛
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
رد دﻮﺟﻮﻣ ي 12
ور ﻊﻳﺎﻣ و اﺪﺟ ﻲﻨﺒﻟ هدﺎﻣ عﻮﻧ ﻳ
ﻲ
ﺘﺷادﺮﺑ ﺎﻬﻧآ ﻪ
؛ﺪﺷ ﻂﻴﺤﻣ رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﺖﺸﻛ زا ﺲﭘ
رﺎﮔآ ﺎﻴﺒﻤﻠﻛ ﻲﺻﺎﺼﺘﺧا ﺖﺸﻛ
، ور ﻊﻳﺎﻣ ﺪﺷر رﺎﻬﻣ ﺮﺛا ﻳ
ﺮﺑ ﻲ
شور ﻪﺑ رﻮﻛﺬﻣ يﺮﺘﻛﺎﺑ Well Diffusion Agar
ﻲﺳرﺮﺑ
ﺪﻳدﺮﮔ . ﻪﻧﻮﻤﻧ ﺎﻫ ﺗ زا شراﻮﮔ ﻦﻴﺼﺼﺨﺘﻣ ﻂﺳﻮ هﺪﻌﻣ
ﻧارﺎﻤﻴﺑ ﻲ
ﺪﻨﺘﺷاد شراﻮﮔ ﻲﻧﺎﻗﻮﻓ ﻲﭘﻮﻜﺳوﺪﻧآ ﻪﺑ زﺎﻴﻧ ﻪﻛ
، رد و ﻪﻴﻬﺗ
تراﻮﺘﺳا ترﻮﭙﺴﻧاﺮﺗ ﺖﺸﻛ ﻂﻴﺤﻣ )
ﺖﻛﺮﺷ لﻮﺼﺤﻣ
Oxoide (
و لﺎﻳو ﭻﻴﭘ رد يﺎﻫ ﺘﻓﺮﮔ راﺮﻗ راد
ﺎﻬﻧآ زا و ﺪﻨ رد
يراﺪﻬﮕﻧ لﺎﭽﺨﻳ ﺪﺷ
. دﻮﺟو ﺎﺑ ﻳا ﻦ ﻪﻛ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ رد
ﺎﺗ قﻮﻓ ﻂﻴﺤﻣ 24
ز ﺖﻋﺎﺳ ﺖﺸﻛ ﻞﺑﺎﻗ و هﺪﻧ ﻲﻣ
ﺪﺷﺎﺑ
، ﻪﻧﻮﻤﻧ ﺎﻫ
هﺎﮕﺸﻳﺎﻣزآ ﻪﺑ ﻪﻠﺻﺎﻓﻼﺑ ﻞﻘﺘﻨﻣ
هداد ﺖﺸﻛ ﺖﺻﺮﻓ ﻦﻴﻟوا رد و
ﺪﻧﺪﺷ . ﻪﻧﻮﻤﻧ ﺎﻫ ﺖﺸﻛ توﺎﻔﺘﻣ شور ود ﻪﺑ هداد
ﺪﻧﺪﺷ :
1 - ﻞﻳﺮﺘﺳا مﻻ يور لوا شور رد 1
- 2 مﺮﺳ هﺮﻄﻗ
ﻞﻳﺮﺘﺳا يژﻮﻟﻮﻳﺰﻴﻓ
؛ﺪﺷ ﻪﺘﺨﻳر ﻞﻳﺮﺘﺳا پﻮﻟ ﺎﺑ ﻪﻧﻮﻤﻧ ﺲﭙﺳ
مﻻ ﻪﺒﻟ ﺎﺑ و ﻪﺘﺷاﺬﮔ مﻻ يور دﺮﺧ يﺮﮕﻳد ﻞﻳﺮﺘﺳا
ﺪﺷ ﺎﺗ
ﺑ ﺖﺧاﻮﻨﻜﻳ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ﻪ
ﺖﺳد آﻳ ﺪ
؛ زا پﻮﻟ ﻚﻳ ﺲﭙﺳ ﻳا
ﻦ
ﻪﺘﺷادﺮﺑ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ رﺎﮔآ ﺎﻴﺒﻤﻠﻛ ﻂﻴﺤﻣ رد و
) لﻮﺼﺤﻣ
Quelab Europe ﺖﻛﺮﺷ (
ﻪﻛ ﻲﺘﻧآ و ﻞﻤﻜﻣ يوﺎﺣ ﻚﻴﺗﻮﻴﺑ
،دﻮﺑ ﺖﺸﻛ ﺪﺷ هداد . مﺎﺠﻧا نﺎﻣز ﻦﻳﺮﺘﻫﺎﺗﻮﻛ رد ﻞﺣاﺮﻣ مﺎﻤﺗ
ﺪﺷ .
2 - ﻪﻧﻮﻤﻧ مود شور رد رﻮﻃ ﻪﺑ
ﺴﻣ ﻂﻴﺤﻣ يور ﻢﻴﻘﺘ
راﺮﻗ ﺖﺸﻛ ﺪﺷ هداد
ﺲﭙﺳ ؛ ﻂﻴﺤﻣ يور پﻮﻟ زا هدﺎﻔﺘﺳا ﺎﺑ
هداد ﺖﻛﺮﺣ ﺖﺸﻛ ﺪﺷ
ﻲﺳﻮﻛﻮﻣ ﻪﻳﻻ ﺎﺗ ﻞﻣﺎﻛ رﻮﻃ ﻪﺑ
يور رد
دﻮﺷ بﺬﺟ و ﻢﻀﻫ ﻂﻴﺤﻣ .
مود شور ﺮﻈﻧ ود زا
دﻮﺑ ﺢﺟرا
؛ ﻲﻜﻳ ﻳا ﻦ ﻪﻛ ﻪﻧﻮﻤﻧ نﺎﻣز
ﻛ يﺮﺘﻤ دﻮﺑ نﮋﻴﺴﻛا ضﺮﻌﻣ رد و
مود ﻳا ﻦ ﻲﮔدﻮﻟآ لﺎﻤﺘﺣا ﻪﻛ
دﻮﺑ ﺮﺘﻤﻛ . زا ﺲﭘ ﺖﺸﻛ
، ﺖﻴﻠﭘ ﺎﻫ ﻞﻴﻓوﺮﺋآوﺮﻜﻴﻣ ﻂﻳاﺮﺷ رد
O2
5
% N2، 85 % CO2 و 10 % يﺎﻣد و 37°c
تﺪﻣ ﻪﺑ 3 - 7
رد زور ﻲﺑ رﺎﺟ راﺮﻗ يزاﻮﻫ
؛ﺪﻨﺘﻓﺮﮔ يﺮﺘﻛﺎﺑ ﺪﺷر ترﻮﺻ رد
،
ﻲﻨﻠﻛ ﺎﻫ
ﺪﻣ ي رو
،
ﺪﺤﻣ و فﺎﻔﺷ ﺮﻫﺎﻇ ب
ﻲﻣ ﺪﺷ ﻦﻳا رد ؛ﺪﻧ
٢١
ترﻮﺻ ﻞﻣﺎﺷ يﺪﻌﺑ تﺎﻣاﺪﻗا يﺎﻬﻧﻮﻣزآ
ﻲﺼﻴﺨﺸﺗ و يﺪﻴﻳﺎﺗ
ﻲﻣ مﺎﺠﻧا ﻧﻮﻣزآ ﻦﻳا ؛ﺪﺷ
ﻞﻣﺎﺷ ﺎﻬ ﮓﻧر
،مﺮﮔ يﺰﻴﻣآ ﺶﻳﺎﻣزآ
هروا ) ﺖﺒﺜﻣ زآ هروا (
، ﺶﻳﺎﻣزآ زاﺪﻴﺴﻛا ) ﺖﺒﺜﻣ زاﺪﻴﺴﻛا (
زﻻﺎﺗﺎﻛ ،
) ﺖﺒﺜﻣ زﻻﺎﺗﺎﻛ (
دﻮﺑ .
ﺶﻳﺎﻣزآ زآ هروا ﺐﺴﻛ رﻮﻈﻨﻣ ﻪﺑ ، ﮓﻧر زا نﺎﻨﻴﻤﻃا
يﺰﻴﻣآ
مﺮﮔ
، مﺎﺠﻧا ﺪﺷ . ياﺮﺑ
،ﺶﻳﺎﻣزآ ﻦﻳا مﺎﺠﻧا ﻲﻤﻛ راﺪﻘﻣ
راﺮﻗ هروا ﻂﻴﺤﻣ رد هﺪﺷ ﻪﻴﻬﺗ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ﻲﻣ
ﺖﻓﺮﮔ و ﻪﺑ
تﺪﻣ 3 ا رد ﺖﻋﺎﺳ ﺗ
° ﻮ 37 ﻪﺑﻮﻜﻧا ﻲﻣ ﺪﺷ و ﺲﭘ يﺎﻀﻘﻧا زا
تﺪﻣ
، ﻲﺳرﺮﺑ ﻪﺠﻴﺘﻧ ﻲﻣ
ﺪﻳدﺮﮔ .
يﺮﺘﻛﺎﺑ ﺪﺷر ترﻮﺻ رد
، نآ ﻲﻨﻠﻛ ﺪﻨﭼ ﻪﺘﺷادﺮﺑ
ﻲﻣ و ﺪﺷ
وﺮﻜﻴﻣ رد لﺎﻳو
رد ﻲﻜﻴﺘﺳﻼﭘ يﺎﻫ ب
يوﺎﺣ ﻞﻳﺮﺘﺳا راد 5cc
/ 1
ثاﺮﺑ يﻮﺳ ﺲﻴﻛ ﻲﺘﻴﭘﺮﺗ ﻂﻴﺤﻣ 15
% لوﺮﺴﻴﻠﮔ راد
) لﻮﺼﺤﻣ
Pronodisha ﺖﻛﺮﺷ (
ﻞﺣ زا و نآ رد
°c يﺎﻣد 70 -
يراﺪﻬﮕﻧ ﻲﻣ
ﺪﺷ .
ﻪﻴﻬﺗ ياﺮﺑ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ
،
دﺎﻣ عﻮﻧ ﺪﻨﭼ ه
ﻪﻴﻬﺗ ﻲﻨﺒﻟ ﺪﻳدﺮﮔ
و
هزاﺪﻧا ﻪﺑ ماﺪﻛ ﺮﻫ زا 3
ﻪﻟﻮﻟ رد پﻮﻟ ﺎﻫ
ﺖﺸﻛ ﻂﻴﺤﻣ يوﺎﺣ ي
Mrs Broth )
ﺖﻛﺮﺷ لﻮﺼﺤﻣ Pronodisha
( ﻞﺣ
؛ﺪﺷ
ﻪﻟﻮﻟ ﺲﭙﺳ ﺎﻫ
آوﺮﻜﻴﻣ ﻂﻳاﺮﺷ رد ﺋ
تﺪﻣ ﻪﺑ ﻞﻴﻓوﺮ 18
رد ﺖﻋﺎﺳ
يﺎﻣد 37°c ﻪﺑﻮﻜﻧا ﺪﻳدﺮﮔ .
يﺮﺘﻛﺎﺑ ﺪﺷر زا ﺲﭘ )
رﺪﻛ ﻂﻴﺤﻣ نﺪﺷ (
، ﻪﻴﻬﺗ ياﺮﺑ
ﺺﻟﺎﺧ يﺮﺘﻛﺎﺑ
، ﺘﺷادﺮﺑ ﻂﻴﺤﻣ زا پﻮﻟ ﻚﻳ و ﻪ
ﺖﻴﻠﭘ يور ﺎﻫ
ي
MRS-Agar ﺖﺸﻛ
ﺪﺷ هداد
؛ ﻞﻴﻓوﺮﺋآوﺮﻜﻴﻣ ﻂﻳاﺮﺷ رد ﻂﻴﺤﻣ
و
°cيﺎﻣد 37 تﺪﻣ ﻪﺑ 18 ﺖﻋﺎﺳ ﻪﺑﻮﻜﻧا ﺲﭙﺳ ؛ﺪﺷ ﻲﻨﻠﻛ
يﺎﻫ
يﺮﻴﺷ ﺎﻳ ﻚﭼﻮﻛ ﮓﻧر
، ﺪﺷر ترﻮﺻ رد ﻞﻣﺎﻛ هرﺎﻨﻛ ﺎﺑ
، ﺖﻬﺟ
ﻣد رد هﺮﻴﺧذ
°c يﺎ 70 - ﻂﻴﺤﻣ ﻪﺑ Skim Milk
12 %
) ﺖﻛﺮﺷ لﻮﺼﺤﻣ (Biomark
ﻲﻣ ﻞﻘﺘﻨﻣ ﺪﺷ
.
ﻪﻛ ﺖﺳا ﺮﻛذ ﻪﺑ مزﻻ ﻞﺣاﺮﻣ مﺎﻤﺗ رد
ﺐﺴﻛ رﻮﻈﻨﻣ ﻪﺑ
زا نﺎﻨﻴﻤﻃا ﻞﻴﺳﺎﺑﻮﺘﻛﻻ
ندﻮﺑ يﺮﺘﻛﺎﺑ ﺎﻫ
، زا يﺎﻬﻧﻮﻣزآ و يﺪﻴﻳﺎﺗ
ﻣ ﻲﺼﻴﺨﺸﺗ ﺜ
ﮓﻧر ﻞ مﺮﮔ يﺰﻴﻣآ )
ﻞﻴﺳﺎﺑ ياﺮﺑ مﺮﮔ ،ﺪﻨﻠﺑ يﺎﻫ
رﻮﭙﺳا نوﺪﺑ ،ﺖﺒﺜﻣ (
، ﮓﻧر تﺮﺒﻟآ يﺰﻴﻣآ )
ﻞﻴﺳﺎﺑ ياﺮﺑ يﺎﻫ
ﻪﻧاد ياراد ﺎﻫ
ﻚﻴﺗﺎﻣوﺮﻛﺎﺘﻣ ي (
و نﻮﻣزآ هدﺎﻔﺘﺳا زﻻﺎﺗﺎﻛ
ﺪﺷ .
ﻚﻳ ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ نﻮﻣزآ زا هدﺎﻔﺘﺳا ﺎﺑ تﺎﻋﻼﻃا رد ﻪﻓﺮﻃ
ﻲﻨﻌﻣ ﺢﻄﺳ يراد
05 / P≤0 ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻞﻴﻠﺤﺗ درﻮﻣ ، .
ﻪﺘﻓﺎﻳ ﺎﻫ ﺮﻄﻗ ﻦﻴﮕﻧﺎﻴﻣ
ﺎﻫ رد ﺪﺷر مﺪﻋ ﻪﻟ دراﻮﻣ مﺎﻤﺗ
1 /
±3 6 / 7
ﺮﺘﻤﻴﻠﻴﻣ دﻮﺑ ) 05 / P<0 ( . ﻚﻳ ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ نﻮﻣزآ رد ﻪﻓﺮﻃ
درﻮﻣ 12 ﻲﻨﻌﻣ ﻲﻨﺒﻟ ﻒﻠﺘﺨﻣ هدﺎﻣ دﻮﺑ راد
) لوﺪﺟ 1 (.
ﺮﺘﺸﻴﺑ
ﻪﻧﻮﻤﻧ ﺎﻫ ور ﻊﻳﺎﻣ ﻂﺳﻮﺗ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ي ﻳ
زا ﻞﺻﺎﺣ ﻲ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺖﺸﻛ ﺎﻫ
ﺪﻧﺪﺷ رﺎﻬﻣ .
هﺰﻳرﻮﺘﺳﺎﭘ ﺮﻴﻏ ﻲﻨﺒﻟ داﻮﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦﻴﮕﻧﺎﻴﻣ ) 9 /
±2 2 / 8 ( هﺰﻳرﻮﺘﺳﺎﭘ داﻮﻣ زا ﺮﺘﺸﻴﺑ ، )
4 /
±3 5 / 7 ( ؛دﻮﺑ
رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦﻴﮕﻧﺎﻴﻣ ﻦﻴﻨﭽﻤﻫ ﻲﻨﺒﻟ تﻻﻮﺼﺤﻣ زا هﺪﺷاﺪﺟ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻲﻳور ﻊﻳﺎﻣ ﺮﺑاﺮﺑ ﻲﻠﺤﻣ ) 8 /
±1 9 / 8 ( ﻲﻠﺤﻣ ﺮﻴﻏ زا ﺮﺘﺸﻴﺑ )
6 /
±3 2 / 7 ( دﻮﺑ .
ﺚﺤﺑ ﻢﻳﺪﻗ زا ﺮﺑ نآ تاﺮﺛا و ﻲﻨﺒﻟ داﻮﻣ زا هدﺎﻔﺘﺳا مﺎﻳﻻا
نژﻮﺗﺎﭘ هدور يﺎﻫ ا هدﻮﺑ ﺚﺤﺑ درﻮﻣ و حﺮﻄﻣ يا
زا ﻲﻟو ﺖﺳ
ﺑ نﺮﻗ ﻞﻳاوا ﻴ
هدﺎﻔﺘﺳا و ﻪﻴﻬﺗ رد و ﺪﺷ يﺮﺘﺸﻴﺑ ﻪﺟﻮﺗ نآ ﻪﺑ ﻢﺘﺴ
زا نآ ناﻮﻨﻋ ﺖﺤﺗ ﻚﻴﺗﻮﻴﺑوﺮﭘ
ﺖﻓﺮﮔ ترﻮﺻ ﺮﻓاو ﻲﻌﺳ ﺎﻫ .
لوﺪﺟ 1 - ﻣ ﻴ ﮕﻧﺎ ﻴ ﻠﻫ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫﺮﻄﻗ ﻦ ﻴ
ﺮﺘﻛﺎﺑﻮﻜ ﻴﭘ روﺎﺠﻣ رد يرﻮﻠ ت
ﺎﻣ ﻊﻳ ور ﻲﻳ ﺳﺎﺑﻮﺘﻛﻻ ﻴ جاﺮﺨﺘﺳا ﻞ ﻒﻠﺘﺨﻣ ﻲﻨﺒﻟ داﻮﻣ زا هﺪﺷ
در ﻳ ﻒ ﻨﺒﻟ عﻮﻧ ﻴ تﺎ مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ
ﺪﺷر داﺪﻌﺗ ﻪﻧﻮﻤﻧ
رﺎﻴﻌﻣ فاﺮﺤﻧا و ﻦﻴﮕﻧﺎﻴﻣ
1 ﻜﭼ ﺖﺳﺎﻣ ﻴ هﺪ 6
/
±1 1 / 10 20
2 ﻲﻠﺤﻣ ﺖﺳﺎﻣ
7 /
±1 9 / 9 20
3 ﺷ
ﻴ ﺮ 9
/
±1 8 / 9 20
4 ﻨﭘ ﻴ ﻲﻠﺤﻣ ﺮ 8
/
±1 3 / 9 20
5 هﺰﻳرﻮﺘﺳﺎﭘ ﺮﻴﻨﭘ
4 /
±1 0 / 9 20
6 ﺮﻴﺷﺮﺳ ﻲﻠﺤﻣ 4
/
±1 9 / 8 20
7 ﺖﺳﺎﻣ رﻮﺘﺳﺎﭘ ﻳ هﺰ 8
/
±1 9 / 8 20
8 ﻪﻣﺎﺧ ﻲﻠﺤﻣ 3
/
±1 4 / 8 20
9 ﻠﺤﻣ هﺮﻛ بآ ﻲ
3
/
±2 2 / 8 20
10 رﻮﺘﺳﺎﭘ ﻪﻣﺎﺧ ﻳ
هﺰ 6
/
±3 6 / 6 20
11 غود 0
/
±3 5 / 3 20
12 ﻚﺸﻛ 9
/
±2 0 / 3 20
٢٢ ﻢﺴﻴﻧﺎﮔرا ﻒﻴﺻﻮﺗ ياﺮﺑ رﺎﺑ ﻦﻴﻟوا ﻚﻴﺗﻮﻴﺑوﺮﭘ ترﺎﺒﻋ ﺎﻫ
و
ﻞﻣاﻮﻋ مﻮﻬﻔﻣ ﻪﺑ ﻪﻛ ﺪﺷ ﻪﺘﻓﺮﮔرﺎﻜﺑ اﺬﻏ ﻪﺑ ﻲﻧدوﺰﻓا داﻮﻣ لدﺎﻌﺘﻣ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ هﺪﻨﻨﻛ ﺎﻫ
و نﺎﺴﻧا شراﻮﮔ هﺎﮕﺘﺳد ي
ناﻮﻴﺣ ﻲﻣ ﺪﺷﺎﺑ .
ﺑوﺮﭘ ﻚﻴﺗﻮﻴ ﺎﻫ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻞﻣﺎﺷ
، ،سﻮﻟﻮﻜﻳﺪﭘ
مﻮﻳﺮﺘﻛﺎﺑ و ﺪﻴﻔﻴﺑ سﻮﻛﻮﻛﻮﺘﭘﺮﺘﺳا ﻲﻣ
ﺪﺷﺎﺑ . ﻳا ﻦ يﺮﺘﻛﺎﺑ ﺎﻫ
ﻲﻣ نژﻮﺗﺎﭘ زا ﻲﻌﻴﺳو ﻒﻴﻃ ﺪﺷر ﺪﻨﻧاﻮﺗ ﺎﻫ
هدور ي يا و نﺎﺴﻧا رد
ﺪﻨﻨﻛ رﺎﻬﻣ ار تﺎﻧاﻮﻴﺣ .
ﺪﻧور ﻲﻟﺎﻤﺘﺣا ﻳا ﻞﻤﻋ ﻦ
، تﻻﻮﺼﺤﻣ ﺮﻳﺎﺳ و ﺪﻴﺳا ﺪﻴﻟﻮﺗ
ﻢﺴﻴﻟﻮﺑﺎﺘﻣ زا ﻞﺻﺎﺣ يﺮﺘﻛﺎﺑ
ﺎﻫ ﺖﺳ . ﺪﻴﻟﻮﺗ ﻂﺳﻮﺗ ﻚﻴﺘﻛﻻﺪﻴﺳا
ﻳا ﻢﺴﻴﻧﺎﮔرا ﻦ ﺎﻫ
رﺎﻬﻣ ﺚﻋﺎﺑ رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ
نﺎﺴﻧا
ﻲﻣ دﻮﺷ ) 12 ( .
Midolo
،نارﺎﻜﻤﻫ و ﺮﺑ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻲﻋﻮﻧ ﺮﺛا
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ار
ﻲﺳرﺮﺑ ﻲﻫﺎﮕﺸﻳﺎﻣزآ ﻂﻳاﺮﺷ رد شراﺰﮔ و
ﺪﻧدﺮﻛ يﺪﻴﻟﻮﺗ ﻚﻴﺘﻛﻻﺪﻴﺳا ﻪﺑ طﻮﺑﺮﻣ يرﺎﻬﻣ ﺮﺛا ﻦﻳﺮﺘﺸﻴﺑ ﻪﻛ
يﺮﺘﻛﺎﺑ ﺎﻫ هدﻮﺑ ﻦﻴﺑ رد و ﻞﻴﺳﺎﺑﻮﺘﻛﻻ
ﺎﻫ L-casei ، ﺮﺛا ﻦﻳﺮﺘﺸﻴﺑ
ﻲﮔﺪﻧرادزﺎﺑ ار
يور يﺮﺘﻛﺎﺑ ﺎﻫ اد ﺖﺳا ﻪﺘﺷ )
13 (
؛ ﻦﻴﻨﭽﻤﻫ ﺞﻳﺎﺘﻧ
ﻪﻌﻟﺎﻄﻣ Graciela نارﺎﻜﻤﻫ و
ﻪﻛ نآ رد يرﺎﻬﻣ ﺮﺛا
سﻮﻠﻴﻓوﺪﻴﺳا ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺮﺑ
رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ يور
ﻲﺳرﺮﺑ هﺎﮕﺸﻳﺎﻣزآ
،ﺪﻳدﺮﮔ تﺎﻌﻟﺎﻄﻣ ﺎﺑ قﻮﻓ
ﺖﺷاد ﻲﻧاﻮﺨﻤﻫ
) 14 .(
Canducci ﻫ و
نارﺎﻜﻤ ﺰﻴﻧ
ﺪﻧدﺮﻛ شراﺰﮔ ﻪﻛ
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﺮﺑ يرﺎﻬﻣ ﺮﺛا سﻮﻠﻴﻓوﺪﻴﺳا ﻞﻴﺳﺎﺑﻮﺘﻛﻻ دراد
؛ ﻳا ﻘﻘﺤﻣ ﻦ نﺎ نارﺎﻤﻴﺑ زا ﻲﻫوﺮﮔ ﻪﺑ ﻪﺳ
ﻲﺘﻧآ عﻮﻧ ﻚﻴﺗﻮﻴﺑ
ﺮﮕﻳد هوﺮﮔ ﻪﺑ و لﺎﻌﻓ ﺮﻴﻏ سﻮﻠﻴﻓوﺪﻴﺳا ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﺑ هاﺮﻤﻫ ﻂﻘﻓ ﻪﺳ ﺰﻳﻮﺠﺗ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﺪﻧدﺮﻛ
ﻪﺑ ﻲﺳرﺮﺑ زا ﺲﭘ و
ﻳا ﻦ
ﺪﻴﺳر ﻪﺠﻴﺘﻧ ﺪﻧ
ﻖﻓﻮﻣ لوا هوﺮﮔ رد نﺎﻣرد ﻪﻛ ﺖﺳا هدﻮﺑ ﺮﺗ
) 15 (.
Felley
،نارﺎﻜﻤﻫ و ﺮﺑ هﺪﺷ شﺮﺗ ﺮﻴﺷ بﻮﻠﻄﻣ ﺮﺛا
ﺖﻳﺮﺘﺳﺎﮔ 35
ور ﻊﻳﺎﻣ و يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﻪﺑ هدﻮﻟآ ﺐﻠﻃواد ﻳ
ﻲ
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﺮﺑ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ار
لاﺪﻴﺴﻳﺮﺘﻛﺎﺑ ﺮﺛا و ﻲﺳرﺮﺑ
نآ دﺮﻛ ﺪﻴﻳﺎﺗ ار ﺪﻧ
) 16 (.
Vilaichone
نارﺎﻜﻤﻫ و رﺎﻬﻣ ﺮﺛا
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻲﻋﻮﻧ ي
ناﺰﻴﻣ و ﻲﺳرﺮﺑ ار هﺎﮕﺸﻳﺎﻣزآ رد يرﻮﻠﻴﭘ ﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ يور ﺮﺑ ﺮﺑ يرﺎﻬﻣ ﺮﺛا
ﻳا ﺖﺸﻛ ﻂﻴﺤﻣ رد ار ﺎﻬﻧآ ﺪﺷر مﺪﻋ و يﺮﺘﻛﺎﺑ ﻦ
دﻮﻤﻧ ﺪﻴﻳﺎﺗ ﺪﻧ
) 17 (.
،نارﺎﻜﻤﻫ و Cats يوﺎﺣ ﺮﻴﺷ رﺎﻬﻣ ﺮﺛا
L-Casei ﺮﺑ
ﻢﻛ و ﻲﺳرﺮﺑ ار هﺎﮕﺸﻳﺎﻣزآ و هﺪﻌﻣ رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ نﺪﺷ
ار ﺎﻬﻧآ ﺖﺸﻛ ﻂﻴﺤﻣ رد ار يﺮﺘﻛﺎﺑ ﺪﺷر مﺪﻋ و هﺪﻌﻣ رد شراﺰﮔ
ﺪﻧدﺮﻛ ) 18 ( . ﻲﻣ نﺎﺸﻧ ﺮﮕﻳد تﺎﻌﻟﺎﻄﻣ ﻲﺧﺮﺑ ﺞﻳﺎﺘﻧ ﻪﻛ ﺪﻫد
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﺪﺷر ﻊﻧﺎﻣ ﻒﻠﺘﺨﻣ ي ﻲﻣ
ﺪﻧﻮﺷ
) 20،19 (
؛ ﺗ ﻦﻴﻨﭽﻤﻫ ﺄ
يور ﺮﺑ تﺎﻴﻨﺒﻟ رد دﻮﺟﻮﻣ ﻞﻣاﻮﻋ ﺮﻴﺛ
يﺮﺘﻛﺎﺑ ﺎﻫ هدور ي يا و ﺰﻴﻧ يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﻪﻴﻠﻋ شراﺰﮔ
ﺖﺳا هﺪﺷ )
21 - 23 (.
رد ﻲﺳرﺮﺑ
،ﺮﺿﺎﺣ ﻊﻳﺎﻣ يرﺎﻬﻣ ﺮﺛا ﻲﺳرﺮﺑ و ﺮﻴﺜﻜﺗ زا ﺲﭘ
ور ﻳ ﺮﺑ ﻲﻨﺒﻟ تﻻﻮﺼﺤﻣ ﻲ 20
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ درﻮﻣ هﺪﺷاﺪﺟ
زا ﻪﻧﻮﻤﻧ نارﺎﻤﻴﺑ هﺪﻌﻣ
، ﺨﺸﻣ ﺺ ﻲﻨﺒﻟ داﻮﻣ ﻦﻴﺑ رد ﻪﻛ ﺪﺷ درﻮﻣ
ﻪﻌﻟﺎﻄﻣ
، ﻳﺮﺘﺸﻴﺑ ﻦ ﻦﻳﺮﺘﻤﻛ و ور ﻊﻳﺎﻣ ﻪﺑ طﻮﺑﺮﻣ ﺮﺛا
ﻳ ﻲ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
زا هﺪﺷ اﺪﺟ ي ﺖﺳﺎﻣ
و هﺪﻴﻜﭼ ﻚﺸﻛ
ﺖﺳا .
ﺖﺳا ﺮﻛذ ﻪﺑ مزﻻ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺲﻨﺟ
ا ﺎﺑ ﻲﻨﺒﻟ داﻮﻣ ﺐﻠﻏ
دراد توﺎﻔﺗ ﻢﻫ .
ﺶﻨﻛاو رد فﻼﺘﺧا ﺚﻋﺎﺑ عﻮﺿﻮﻣ ﻦﻳا ﻳا
ﻦ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺎﻫ
يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﻪﺑ ﺖﺒﺴﻧ ﻲﻣ
دﻮﺷ . زا ﻲﻓﺮﻃ
لﻮﺼﺤﻣ عﻮﻧ ﻲﻣ
ﺪﺷﺎﺑ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻲﺻﺎﺧ عﻮﻧ ياراد ﺪﻧاﻮﺗ .
ﺮﮕﻳد ﻳا ﻦ ﺒﻟ تﻻﻮﺼﺤﻣ ﺮﮔا ﻪﻛ ﻲﻨ
د ،ﺖﺳﺎﻣ تﺎﺠﺘﺳد ر
ﻲﺳرﺮﺑ ﻚﺸﻛ و ﻪﻣﺎﺧ ،ﺮﻴﺷ دﻮﺷ
، ﻦﻳﺮﺘﺸﻴﺑ ﻦﻳﺮﺘﻤﻛ و
ﺮﺛا ﻪﺑ
ﺐﻴﺗﺮﺗ ﻚﺸﻛ و ﺮﻴﺷ ﻪﺑ طﻮﺑﺮﻣ ﻲﻣ
ﺪﺷﺎﺑ .
ﻖﻴﻘﺤﺗ رد
،ﺮﺿﺎﺣ ﺗﺄ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﺮﻴﺛ ﺎﻫ
داﻮﻣ رد دﻮﺟﻮﻣ ي
اﺬﻏ ﻳ ﺸﻨﻣ سﺎﺳا ﺮﺑ ﻲﻨﺒﻟ ﻲ ﺄ
ندﻮﺑ هﺰﻳرﻮﺘﺳﺎﭘ هﺮﻴﻏ و هﺰﻳرﻮﺘﺳﺎﭘ
ﮕﻧﺎﻴﻣ هﺰﻳرﻮﺘﺳﺎﭘ ﺮﻴﻏ ﻲﻨﺒﻟ داﻮﻣ ﻪﻛ ﺪﺷ ﺺﺨﺸﻣ و ﻪﺴﻳﺎﻘﻣ ﻦﻴ
ﺮﻄﻗ ﺎﻫ ﺪﻧراد هﺰﻳرﻮﺘﺳﺎﭘ داﻮﻣ ﻪﺑ ﺖﺒﺴﻧ يﺮﺘﺸﻴﺑ ﺪﺷر مﺪﻋ ﻪﻟ
؛
ﺪﻧراد يﺮﺘﻬﺑ ﺮﺛا هﺰﻳرﻮﺘﺳﺎﭘ ﺮﻴﻏ ﻲﻨﺒﻟ داﻮﻣ ﻲﻨﻌﻳ ﻦﻳاﺮﺑﺎﻨﺑ ؛
ﻲﻣ ﻪﺠﻴﺘﻧ ﻦﻴﻨﭼ ناﻮﺗ ﻨﺒﻟ داﻮﻣ ﻪﻛ دﺮﻛ يﺮﻴﮔ
ﻲ هﺰﻳرﻮﺘﺳﺎﭘ ﺮﻴﻏ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ياراد ﺎﻫ
و ﻲّﻠﻛ رﻮﻃ ﻪﺑ ﻢﺴﻴﻧﺎﮔرا
ﺎﻫ ﻚﻴﺗﻮﻴﺑوﺮﭘ ي
٢٣
ﻣ ﺆ ﻟ داﻮﻣ ﻪﺑ ﺖﺒﺴﻧ يﺮﺘﺸﻴﺑ ﺎﻳ و ﺮﺛ ﺪﻨﺘﺴﻫ هﺰﻳرﻮﺘﺳﺎﭘ ﻲﻨﺒ
؛
ﻦﻴﻨﭽﻤﻫ ﺮﻄﻗ
ﺎﻫ ﻊﻳﺎﻣ ﺮﺑاﺮﺑ رد يرﻮﻠﻴﭘﺮﺘﻛﺎﺑﻮﻜﻴﻠﻫ ﺪﺷر مﺪﻋ ﻪﻟ
ور ﻳ زا ﺮﺘﺸﻴﺑ ﻲﻠﺤﻣ ﻲﻨﺒﻟ تﻻﻮﺼﺤﻣ زا هﺪﺷ اﺪﺟ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻲ
دﻮﺑ ﻲﻠﺤﻣ ﺮﻴﻏ .
ﻢﻬﻣ ﻪﺘﻜﻧ ﻳا
ﻪﻛ ﺖﺳا ﻦ ﺮﺘﺸﻴﺑ
درﻮﻣ ﻲﻨﺒﻟ تﻻﻮﺼﺤﻣ
ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ياراد ﺎﻣ رﻮﺸﻛ رد هدﺎﻔﺘﺳا ﺎﻫ
ﻚﻴﺗﻮﻴﺑوﺮﭘ ي
ﻲﻣ ﺪﻨﺷﺎﺑ ا ﻦﻴﺑ رد رود ﻪﺘﺷﺬﮔ زا ﺎﻬﻧآ هﺮﻣزور فﺮﺼﻣ و ﻗ
رﺎﺸ
ﺖﺳا هدﻮﺑ ﻲﺘﻣﻼﺳ ﻆﻔﺣ ﻞﻣﺎﻋ مدﺮﻣ و
ﻪﭽﻧﺎﻨﭼ رد مدﺮﻣ ﻲﻫﺎﮔآ
ﺪﺑﺎﻳ ﺶﻳاﺰﻓا ﻪﻌﻣﺎﺟ
، زا هدﺎﻔﺘﺳا ﻪﺑ ﻞﻳﺎﻤﺗ ا
ﻲﻳاﺬﻏ داﻮﻣ ﻦﻳ
ﺖﻓﺎﻳ ﺪﻫاﻮﺧ ﺶﻳاﺰﻓا ﻲﻌﻴﺒﻃ
؛ رد ﻳا ترﻮﺻ ﻦ ﻲﻣ
زا هدﺎﻔﺘﺳا ناﻮﺗ
اﺮﺛا ﺪﻗﺎﻓ و ﻲﺘﻌﻨﺻ يﺎﻫاﺬﻏ فﺮﺼﻣ ﻦﻳﺰﮕﻳﺎﺟ ار تﺎﻴﻨﺒﻟ ﺪﻴﻔﻣ ت
دﺮﻛ . ﺮﺑ هوﻼﻋ
ﻳا رد داﺮﻓا ﻪﻛ زوﺮﻣا ﻲﻨﻴﺷﺎﻣ ﻲﮔﺪﻧز رد ﻦ
ﻋ عاﻮﻧا ضﺮﻌﻣ ﻮ
يرﺎﻤﻴﺑ ﻞﻣا ﺪﻨﺘﺴﻫ از
، يﺎﻬﻫار زا ﻪﻛ ﺖﺳا ﻢﻬﻣ
ﻲﻌﻴﺒﻃ
، آ دﻮﺷ يﺮﻴﮕﺸﻴﭘ ﺎﻬﻳرﺎﻤﻴﺑ ﻪﺑ ﻼﺘﺑا زا نازرا و نﺎﺳ
زا ﻲﻜﻳ ﻪﻛ
ﻳا ﺮﻴﺷ ﻪﻠﻤﺟ زا ﻲﻨﺒﻟ داﻮﻣ فﺮﺼﻣ ﺎﻬﻫار ﻦ
ﻲﻣ ﺪﺷﺎﺑ . ﻪﺠﻴﺘﻧ
يﺮﻴﮔ
ﺎﺣ ﺞﻳﺎﺘﻧ سﺎﺳا ﺮﺑ زا ﻞﺻ
ﻳا ﻪﻴﺻﻮﺗ ﺮﻳز دراﻮﻣ ﻖﻴﻘﺤﺗ ﻦ
ﻲﻣ دﻮﺷ :
- ﻚﻴﺗﻮﻴﺑوﺮﭘ يوﺎﺣ ﻪﻛ ﻲﻨﺒﻟ داﻮﻣ زا نوﺰﻓا زور هدﺎﻔﺘﺳا
ﺪﻨﺷﺎﺑ .
- ﺎﺳﺎﻨﺷ ﻳ اﺬﻏ داﻮﻣ ﻲ ﻳ
ﻢﺴﻴﻧﺎﮔرا ﻲ ﺪﻴﻔﻣ راد
- اﺬﻏ داﻮﻣ ﻪﻴﻬﺗ ﻳ
ﻣ و ﻦﻳﺮﺘﺸﻴﺑ يوﺎﺣ ﻪﻛ ﻲﻨﺒﻟ ﻲ ﺆ
ﻦﻳﺮﺗﺮﺛ
ﺪﻨﺷﺎﺑ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ .
- ﻪﻓﺎﺿا ندﺮﻛ ﺪﻴﻔﻣ ﻞﻴﺳﺎﺑﻮﺘﻛﻻ ﻪﺑ
ﻲﻨﺒﻟ داﻮﻣ
- ﻧا ﺮﺑ ﻲﻨﺒﻟ داﻮﻣ تاﺮﺛا ﺎﺑ ﻪﻄﺑار رد ﺮﺘﺸﻴﺑ تﺎﻌﻟﺎﻄﻣ مﺎﺠ
نژﻮﺗﺎﭘ ﺎﻫ
و ﺮﻳﺪﻘﺗ ﺮﻜﺸﺗ
زا نﺎﻨﻛرﺎﻛ و ﻲﭘﻮﻜﺳوﺪﻧآ يﺎﻬﺸﺨﺑ مﺮﺘﺤﻣ
بوﺮﻜﻴﻣ ﻢﺋﺎﻗ نﺎﺘﺳرﺎﻤﻴﺑ ﻲﺳﺎﻨﺷ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﺑ ﻪﺘﺴﺑاو
ﻲﻜﺷﺰﭘ يراﺰﮕﺳﺎﭙﺳ و ﺮﻜﺸﺗ ﺪﻬﺸﻣ ﻲﻣ
دﻮﺷ .
ﻊﺑﺎﻨﻣ :
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Role of Lactobacilli emerged from foodstuff on Helicobacter pylori
A. Khosravi Khorashad1, A. Sadeghiyan2, M. Khajeh Karamoddin3, M. Milani4, T. Tavakkoli5
Abstract
Background and Aim: Helicobacter (H.) pylori is responsible for one of the most common bacterial infections in the world. Probiotics such as lactobacilli prevent the multiplication of a wide range of human and animal pathogens. One of the useful potentials of probiotics is their antagonist effect on harmful microorganisms so that they would be effective in prevention and treatment of H. pylori. The present study was aimed to evaluate the inhibitory role of lactobacilli in foodstuff on H. pylori.
Materials and Methods: In this study which carried out in Ghaem medical center of Mashhad University of Medical Sciences in 2003, after culturing 114 enteral biopsies, in 20 samples, H. pylori were obtained. Also, lactobacilli present in 12 kinds of dairy products were discriminated and their supernatants were collected.
After H. pylori was cultured in Colombia-agar culture medium, the inhibitory effect of the supernatants on the bacteria was studied using Well Diffusion Agar Method. The obtained data was analysed by one-sided variance analysis at the significant level P≤0.05 through
Results: Mean diameter of lack of multiplication peripheral circle was 7.6±3.1 mm in all situations (P<0.05).
Most of the H. pylori samples were inhibited by the supernatants. One-sided variance analysis was significant with respect to 12 dairy products. Mean diameter of lack of multiplication peripheral circle of unpasteurized dairy products (8.2±2.9) was more than pasteurized ones (7.5±3.4). Besides, mean diameter of lack of multiplication peripheral circle of H. Pylori against lactobacilli obtained from local dairy products (8.9±1.8) was more than those from outside dairy products (7.2±3.6).
Conclusion: On the basis of the present study, everyday consumption of dairy products having probiotics;
useful microorganisms particularly lactobacilli is effective against H. Pylori. Therefore, providing dairy products with useful lactobacilli is recommended.
Key Words: Helicobacter pylori; Lactobacillus; Probiotics
1 Corresponding Author; Associate Professor, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran [email protected]
2 Associate Professor, Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
3Professor, Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
4 Microbiologist
5Resident, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences. Mashhad, Iran
