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FBC Lett., No. 30 (2009)

20096

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生命化学研究との出会いそれから．．．

東北大学多元物質科学研究所

永次 史

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人工核酸を用いたポリメラーゼ反応

～人工核酸アプタマー創出に向けて～

群馬大学大学院工学研究科

桒原 正靖

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シャペロニンを利用した機能性物質の創製

東北大学多元物質科学研究所

金原 数

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光誘起電子移動に基づく蛍光プローブの精密開発

東京大学大学院薬学研究科

浦野 泰照

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九州大学大学院システム生命科学府

戸井田 力

大阪大学大学院理学研究科

町田 慎之介

東京医科歯科大学生体材料工学研究所

森谷 優貴

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IIS型制限酵素を利用した全自動プラスミドコンストラクション 北海道大学電子科学研究所

小寺 一平

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2nd Switzerland-Japan Biomolecular Chemistry Symposium (SJBCS2009)／国 際バイオ EXPO 特別講演セッション／第 24 回生体機能関連化学シンポジウム・第 12 回バイオテクノロジー部会シンポジウム／FCCA セミナー FCCA グライコサイエン ス若手フォーラム 2009

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受賞・会員異動のお知らせ

編集後記

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1. A.D. Ellington, J.W. Szostak, Nature, 346, 818 (1990).

2. C. Tuerk, L. Gold, Science, 249, 505 (1990).

3. M. Berezovski, M. Musheev, A. Drabovich, S. N. Krylov, J. Am. Chem. Soc., 128, 1410 (2006).

4. A. Nitsche, A. Kurth, A. Dunkhorst, O. Pänke, H. Sielaff, W. Junge, D. Muth, F. Scheller, W. Stöcklein, C. Dahmen, G. Pauli, A. Kage, BMC Biotechnol., 7, 48 (2007).

5. M. Kuwahara, J. Nagashima, M. Hasegawa, T. Tamura, R. Kitagata, K. Hanawa, S. Hososhima, T. Kasamatsu, H. Ozaki, H.

Sawai, Nucleic Acids Res., 34, 5383 (2006).

6. M. Kuwahara, S. Obika, J. Nagashima, Y. Ohta, Y. Suto, H. Ozaki, H. Sawai, T. Imanishi, Nucleic Acids Res., 36, 4257 (2008).

7. S. Obika, D. Nanbu, Y. Hari, K. Morio, Y. In, T. Ishida, T. Imanishi, Tetrahedron Lett. 38, 8735 (1997).

8. M. Kuwahara, H. Takeshima, J. Nagashima, S. Minezaki, H. Ozaki, H. Sawai, Bioorg. Med. Chem., 17, 3782 (2009).

9. ¡PpŽæ–_œÜæ6AF5E7<HMÌ˲æוVÚLã¾¢· 157 (2003).

10. S. M. Freier, K. H. Altmann, Nucleic Acids Res., 25, 4429 (1997).

11. M.M. Masud, M. Kuwahara, H. Ozaki, H. Sawai, Bioorg. Med. Chem., 12, 1111 (2004).

12. A. Shoji, M. Kuwahara, H. Ozaki, H. Sawai, J. Am. Chem. Soc., 129, 1456 (2007).

13. K. Ohsawa, T. Kasamatsu, J. Nagashima, K. Hanawa, M. Kuwahara, H. Ozaki, H. Sawai, Anal. Sci., 24, 167 (2008).

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[1] Braig, K.; Otwinowski, Z.; Hegde, R.; Boisvert, D. C.; Joachimiak A.; Horwich A. L.; Sigler P. B.

Nature 1994, 371, 578-586.

[2] Ishii D.; Kinbara K.; Ishida Y.; Ishii N.; Okochi M.; Yohda M.; Aida T. Nature 2003, 423, 628–632.

[3] Muramatsu S.; Kinbara K.; Taguchi H.; Ishii N.; Aida T. J. Am. Chem. Soc. 2006, 128, 3764–3769.

[4] Shuvendu Biswas S.; Kinbara, K.; Oya N.; Ishii N.; Taguchi, H.; Aida T., J. Am. Chem. Soc. 2009, in press.

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1. Akwasi Minta et al., J. Biol. Chem., 264, 8171-8178 (1989).

2. Tetsuo Miura et al., J. Am. Chem. Soc., 125, 8666-8671 (2003).

3. Kumi Tanaka et al., J. Am. Chem. Soc., 123, 2530-2536 (2001).

4. Naoki Umezawa et al., Angew. Chem. Int. Ed., 38, 2899-2901 (1999).

5. Ken-ichi Setsukinai et al., J. Biol. Chem., 278, 3170-3175 (2003).

6. Tasuku Ueno et al. J. Am. Chem. Soc., 128, 10640-10641 (2006).

7. Suguru Kenmoku et al., J. Am. Chem. Soc., 129, 7313-7318 (2007).

8. Yuuta Fujikawa et al., J. Am. Chem. Soc., 130, 14533-14543 (2008).

No.30 (2009 June) 22

9. Yasuteru Urano et al., J. Am. Chem. Soc., 127, 4888-4894 (2005).

10. Mako Kamiya et al., J. Am. Chem. Soc., 129, 3918-3929 (2007).

11. Tomonori Kobayashi et al., J. Am. Chem. Soc.,129, 6696-6697 (2007).

12. , , 381, 44-50 (2002).

13. , 37, 370-374 (2005).

14. , 57, 699-706 (2006).

15. , 61(9), 23-27 (2006).

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Hood, J. R. Heath, Nat. Biotechnol., 26, 1373-1378 (2008).

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Gourlaouen, M. Salih, M. C. Jones, D. T. Jones, G. Saunders, V. Kostourou, F. P. Sierra, J. C. Norman, G. C.

Tucker, K. M. H. Dilke, Nat. Med., 15, 392-400 (2009).

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H. Terashima, R.A. Yoshizumi, S. Kojima, M. Homma, J. Biochem., 144, 635-642 (2008).

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