AI\IH HUiJI^IG CLIA THIJC Am V A O O IVIARI eEI\J SII\IH TRBJOmo V A TV LE SOUIG CA liHOARJG CO BU
[Amphiprian frenatus Orevoort, 1 S5S) [DUOI e o I \ I G A Y TUOI)
TrIn Van Phudc\ Nguyin Dinh Tnmg\
Vo Thinh Dat^ v l HI L6 Thj Lge^
TOMTAT
Nghien cuu anh huong cua thiic an va do man len sinh tnrong va ty le sdng ca Khoang Co Do dugc tien hanh tai Vien Hai duong hpc Nha Trang tu thang 3 den thang 6/2009. Ket qua nghien ciiu cho thay thiic an la tao, luan tning va copepoda (bp chan kiem) guip ca tang Iruong nhanh, ty le song cao nhat (75,5%) va co mau sic gan mau tu nhien so voi cac loai thuc an khac (p < 0,05). Do man thich hop cho ca phat trien dao dong tli 15%o den 40%o, khoang thich hpp nhat dao dong tir 30%o den 40%o (ty le song > 96%). Tuy nhien, day chi la nghien cuu khao sat ban dau nen can co nhung nghien ciiu sau hon cac yeu to sinh thai den sinh truong, ty le sdng va mau sic ciia ca.
T\t]sh6a.: Anh hudng, do man, thuc dn, ty le sdng. sinh trudng. cd khoang cd dd.
I.MdDAU
Trong nhung nam gin day, nghi nuoi c l canh co nhung budc phlt trien manh va dac biet II c l canh biln do chung co miu sic sac so, hinh dang phong phu. Hau hit cic ddi tugng c l cinh bien deu dugc khai thIc tii tu nhien, rlt it tii sinh sin nhan tao. Qui trinh khai thIc c l cinh biln se Inh hudng rlt ldn din ngudn lgi tir nhien. Cl Khoang Cd la nhdm c l ran san h6, chiing co miu sic tiroi sing vl ve hIi budc khi boi nen chung cdn dugc ggi la ca he. Hien nay, ca Khoang Cd dang duoc nuoi k h i phd biln llm cinh d cac khu du lieh va nhilu hg gia dinh d nude ta. Ca canh bien nuoc ta dugc khai thac, chii yeu d khu vuc miln Tmng va khu vuc vfnh Thai Lan do co nhilu ran san ho. NhQng nam gin day thi tnrdng ca cinh bien phlt trien manh me trong va ngoai nuoc, c l ran san ho hi khai fhIc bu'a bai lam tang nguy co can kiet ngudn lgi sinh vat biln (DIo T i n Hd v l ddng tac gil, 2001). Vi vay, xay dung quy trinh sinh sin nhan tao ca Khoang Cd Dd la rlt cin thief. Bai viet nay gioi thieu kit qui nghien cun Inh hudng cua thuc an va do man den sinh tmdng va ty le sdng cua ca Khoang Co Dd duoi 60 ngay tudi
11. VAT UEU VA PHUOniG P H A P NGHIEN CUU
1. Thdi gian, dia dilm v l ddi tugng nghifin eiiu Nghien cun dugc thuc hien fir thang 3 den thing 6 nam 2009 tai Trai thi nghiem Nghien cun San xult Gidng nhan tao thugc Phong Cong nghe nu6i trdng -
Khoa Nuoi trong Thuy san - Truong Dai hpc Nha Trans Viet Nam;" Vien Hai duang hpc Nha Trang
Vien HIi duong hgc Nha Trang. Ddi tugng nghien cun la c l Khoang Cd Dd (Amphiprion frenatus Brevoort, 1856) co ngudn gdc tii' sinh san nhan tao.
2. Phuong phip thu thIp v l xu ly sd hlu a. So lieu thu cap
Thu thap sd lieu th6ng qua cac bio d o khoa hgc, tai lieu cd lien quan, tap chi khoa hgc trong va ngoli nuoc.
b. So lieu sa cap
Thu thap sd lieu fh6ng qua bd tri thi nghiem, cham soc va quin ly, can do c l thi nghiem.
Nguon nude thi nghiim
Nuoc bien dugc bom vao be ling, sau do bom nud'c fir b l ling vao be chua va xii ly nude bing cio vdi ndng do 30 ppm, giu' nude trong be chira 3 ngay.
Kiem tra du luong cio trong nuoc va khu' bing fhiosulphat truoc khi su' dung.
Bd tri thi nghiim
+ Thi nghiim 1: Anh hudng ciia cac loai thu'c an khac nhau den sinh trudng v l ty le sdng cua ca Khoang Cd Dd (fir 1 din 30 ngay tudi), voi 4 nghiem thuc: NTI (tlo tuoi, luan trung va Nauplius artemia), NT2 (tlo tuoi, luan trung va copepod), NT3 (tao tuoi va thirc an tdng hgp) va NT4 (tao kh6, luan trung va Nauplius artemia).
Be thi nghiem: be kinh co t h i tich 151.
Mat dp tha ca: 30 con/be.
Cac loai thuc an dugc su' dung:
Tao tuoi Nanochoropsis oculata vm mat do 10'' t l bao/ml.
NONG NGHIEP VA PHAT TRIEN NONG THON-SO 1 -THANG 1/2010 87
Luan trimg Brachionus plicatilis vai mat dd 5 - 7 con/ml.
Nauphus cua artemia vdi mat dd 5 - 7 con/ml.
Copepod vdi mat dd 5 - 7 con/ml.
Thuc an tdng hgp vdi ty le 0,2 g/1000 c l fhl.
Bd ti-i thi nghiem: c l Khoang Cd Dd sau khi nd (1 ngly tudi) dugc chuyen vio elc b l thi nghiem, nude d p chu ylu ciia b l thi nghiem tii b l ap d l c l kh6ng hi sdc do moi trudng thay ddi, mdi nghiem thiic lap lai 3 lln. C h i dd cham sdc d 4 nghiem thuc la nhu nhau: h i n g ngay dng truyen nude (dng xi- ph6ng) vl thay nude khoing 20 - 30% lugng nude trong be, kilm tra sd lugng c l chit. Thdi gian cho an:
4 l l n / n g l y vio 8h, 10h30,14h va 16h30.
Thi nghiim 2: Anh hudng cua dd man khIc nhau d i n sinh trudng vl ty le sdng ca Khoang Cd Dd (tii 15 din 60 ngly tudi), vdi 7 nghiem thiic: 5%o, 10%o, 15%o, 20%o, 30%o, 40%o vl ddi chiing II nude bien (dd man dao dong tii 33 - 35%o).
Bl thi nghiem: b l kinh fhl tich 151.
Mat do fhl cl: 25 c o n / b l .
Thuc an: tlo tuoi (mat dd tlo 10^ t l blo/ml), luan tning Brachionus plicatilis vai mat dd 5 - 7 con/ml vl Nauplius artemia (tii 2 - 3 con/ml).
Bd tri thi nghiem: C l Khoang Cd Dd (15 ngly tudi) dugc chuyen vao cac b l thi nghiem, m6i thi nghiem lap lai 3 lln. Quin ly cham sdc: xi-phong, ve sinh b l vl d p them nude moi d d c b l thi nghiem, cho an ngly 2 lln: sang vl chilu.
Cdc thong tin cdn thu thap Cac ylu td m6i trudng
Nhiet do: do bing nhiet k l rugu (do chinh xac 1"C); do man: do b i n g khuc xa k l (do chinh xlc l%o); pH: do bing test pH cam tay; NOs": do bang test NOa'; h i m lugng oxy hda fan (DO): xac dinh bing test so man.
Tang ti-udng ciia c l (kich thudc va khdi lugng).
Tyle sdng ciia ca (%).
C6ng thuc tinh toln •vl xu ly sd li$u - Tdc do tang trudng chilu dli hang ngly:
Le- Ls
DGRL= (mm/ngay) t 2 - t l
- Miic tang chilu dai tuyet ddi:
Lc = Le - Ls (mm)
Trong dd: Ls: chilu dli do lln trudc;
Le: chilu dli do lln sau;
fj- ti: khoing gian giiia 2 lln do (ngly).
- Ty le sdng:
X A
N
noo
Trong dd: A; sd c l cdn lai khi k i t thuc thi nghiem; N: tdng sd c l ban diu.
Sd lieu dugc xii ly theo phuong phip thdng ke sinh hgc tren phln m l m Microsoft Office Excel 2003.
III. KET QUA NGHIEN CUU VA THAO LUAN
1. Anh hudng eua thtie an din sinh trudng vl ty 11 sdng eiia c l (dudi 30 ngly tuoi)
C l Khoang Cd Dd 1 ngly tudi dugc bd fri nglu nhien d 4 nghiem thifc. He thdng nuoi nude hd gdm d c b l kinh cd the tich 15 lit/bl vdi mat dd 30 ca the/lit. C l dugc cho an ngay sau khi nd voi 4 loai
Nghiem thiic NTI
NT2
NT3
NT4
thiic an nhu sau (bing 1):
Bing 1. Qui trinh stt dung thuc 5n trong thi nghiim Loai fhiic an
T l o tuoi Luan trung Nauplius artemia Tao tuoi
Luan trung Copepod T l o tuoi
Thiic an tdng hgp T l o kh6
Luan trung Nauplius artemia
1 ngly tudi 5 ngly tudi 30 ngly tuoi
•
- •
->
a. Mot sd yeu td moi trudng trong thi nghiem Mot sd ylu td m6i trudng trong thi nghiem Inh
hudng cua thiic an d i n ty le sdng va sinh trudng cua ca dugc fhl hien d bing 2.
Bang 2. Mot sd ylu td mdi trudng ti-ong thi
Nhiet
do eo
26-28
DomSn
(%o)
35-36
n g pH 7,9 - 8,3
li^m Oxy h o i tan (mg/l)
5,5-6,5
NH4*
(mg/l) 0-0,1
NO3 (mg/l) 0 - 0,05
Bing 4. Tde do tang trudng ehilu dli d (mm/ngly)
Nhin chung, cac yeu td moi tiirdng p-ong he thdng bl thi nghiem nlm ti-ong gioi han cho phip, thich hgp vdi sir phlt tiiln cua ca (Ha Le Thi Lgc, 2005). Tuy nhien 0 nghiem thu'c NT3 dii ham lugng NH4" va NO3- cd xu huong tang cao hon cac nghiem fhiic khIc.
b. Anh hudng cua thuc dn den tdng trudng chieu ddi cua cd
Tang tmdng chilu dli cua cl chfu Inh hudng ciia nhilu ylu td va h-ong dd, thuc an dong vai h-d quan ti-gng. Kit qui 4 nghiem tiiiic dugc the hien d bing 3.
Bang 3. Chilu dli (mm) eua el a ele nghiem thue thue an khIe nhau
Tudi (ngiy)
1 10 15 20 25 30
Nghifem thuc NTI
4.60 ± 0.00 1I.56±0,81' 15.06± 1.29' 17.27 ±0.70"
18.20 ±0.86"
19.00 ±0,74'
NT2 4.60 ± 0,00 10,74 ±0.66"
14.80 ±0.63"
16.70 ±0.95"
17.90 ±0,71"
18.50 ±0,71"
NTS 4,60 ± 0.00 6,54 ± 1.46"
9.80 ± 1.57"
12.70 ± \.6(f 13,90 ±2.03"
14,50 ±2,99"
NT4 4.60 ±0,00 8.25 ± 0.96' 12.50 ± 1.29' 14,00 ±1.00"' 15,33 ±0.58' 16,50± 1.00' Ghi chii: Sdlieu trinh bdy trong bdng Id gid tri trung binh = do lech chuan. Sdlieu ciing hdng cd cdc cha cdi khde
nhau the Men sai khde cd y nghia thdng ke (p< 0,05).
Kit qui d bing 3 cho fhly: kich thudc ca d NT3 nhd nhlt so voi cl d 3 nghiem thiic cdn lai (p < 0,05) vl ca d nghiem thiic nay cd dd phan din lon (do lech chuln lon). Cl d NTI va NT2 cd chilu dli ldn va dong deu hon so voi 2 nghiem thu'c con lai. Dieu nly cho fhly thiic an cd anh hudng din tang tiirdng chilu dai ciia ca.
Chilu dli cl tang nhanh ding kl a NTI va NT2 so voi NT3 CO fhl do chi do thiic an NT3 kh6ng co luan tiling va ca chua quen voi thuc an tdng hgp trong thdi gian dau. NT4 ca tang tradng cham hon so vdi NTI va NT2 cd thi do NT4 su dung tlo kho nen chat lugng kh6ng tdt bing tlo tuoi nen llm gilm chit lugng artemia. Dilu nly mot lan nira chung minh kef qui ciia Woofton (1995) vl tac dung ciia tao hroi trong viec kich boat he men b-ong ragt cua ca con de cl CO thi bit diu tieu hoi dugc lugng thiic an tuoi sdng dua tii ngoli vio nhu luan tiling, nauplius ciia artemia. Ddng thoi bio tuoi se la ngudn fhiic an cho luan tning hoac artemia trong moi truong nudi.
Tudi (ngiy)
10 15 20 25 30
Nghiem thuc NTI
0,77 0,70 0,44 0,19 0,16
NT2 0,68 0,81 0,38 0,24 0,12
NTS 0,22 0,65 0,58 0,24 0,12
NT4 0,41 0,85 0,30 0,27 0,23
Tdc do tang trudng ciia ca lon d cac nghiem thuc NTI, NT2 d ngay tirdi thu- 15 trong khi do a nghiem thfrc NT3, NT4 ca cd tdc do tang trudng ldn d ngly tudi thu 15 va 20. Nhin chung cl cd tdc dd tang trudng ldn d ngly tudi thii 15 vl sau dd gilm din.
c. Anh hudng cua thuc dn den ty li sdng ciia cd Thiic an II ylu td Inh hudng ldn nhlt din ty le sdng ciia cl dudi 1 thing tudi.
Hinh 1. Ty 1$ sdng cua el a ele nghidm thiic (%) Qua theo doi cho fhly, cl thudng chit nhilu vao tuan diu tien sau khi nd. Dilu nly tirong tir vdi mot sd kit qui nghien ciiu tiirdc day ddi voi lu trimg ca Khoang Cd, ty le sdng xIp xi 50% ti-ong hai tuan diu va chit cao nhlt tii ngly thii 2 vl thu' 8 sau khi nd (Ha Le Thi Lgc, 2002). Ty le sdng ciia cl con trong dilu kien thi nghiem phu thudc vao nhieu ylu td nhu chit lugng tiling, dieu kien m6i tiirdng bl Ip va bl nu6i au tiling. Tuy nhien, kit qua nghien cun cho fhly d c ylu td moi tiirdng (nhiet do, do man, pH, oxy hda tan, NHj^va NO3-) diu nam ti-ong pham vi gioi ban ddi voi cl nu6i (Bang 2), ngudn cl dugc lay tii cung mot d tning nen chat lugng tiling la nhu nhau. Do vay su khIc nhau r5 ret vl ty le sdng giira d c nghiem thuc II do anh hudng bdi cac loai thiic an khac nhau.
Tir hinh 1 tii fhly ty le sdng ciia d c I6 thi nghiem CO su khIc biet ro ret, chia lam 2 nhdm: NTI, NT2 va NT4 CO ty le sdng cao hon nhieu so voi NT3 la 21,8%.
Ca Khoang Cd Dd mdi nd co xu huong bat mdi sdng nen sir khac biet ti-en la do NTI, NT2 va NT4 sii dung luan tning dl cho d an fir ngay dau den 5 ngay tirdi, NT3 su dung thtic an tdng hgp ngay tii ngay dau
NONG NGHIEP VA PHAT TRIEN NONG THON - SO 1 - THANG 1/2010 89
nen ca chua quen thuc an la. C l c h i t nhilu tirong 3 ngly dau d NTS va quan sit tren kinh hien vi thi thay kh6ng CO thiic an tdng hop trong da dly ca, chi c6 1 if tlo. Dilu nay cho fhly c l d NT3 chit do thilu thu'c an vi c l chua quen bat mdi tinh vl thuc an khong lien tuc trong m6i trudng sdng ciia cl. O NT4 fv' le sdng cao (65,5%), tao kh6 co the sii dung thay cho tao tuoi llm thuc an cho ca va artemia, copepod, giup chuan bi thiic an cho c l d l ding hon.
Kef qui tren cho fhly, luan trung la thuc an can thilt cho c l bgt trong 5 ngly diu, chiing cd anh hudng quylt dinh d i n ty le sdng cua c l bgt. Tao tuoi cd vai fro dn dinh m6i truong b l nu6i, lam thuc an cho luan trung, copepoda va Nauplius artemia, ngoli ra con llm thiic an true tilp cho cl.
d. Anh hudng cua thiic dn din mdu sac cd
O NT2, ca cd mau dd tuoi dep nhat (gidng miu tu nhien), c l d cac nghiem thirc khac cd miu cam nhat hon. Nguyen nhan II do NT2 sii dung copepod llm thuc an d l uong nudi gidng vdi thiic an tu nhien ciia ca.
Qua k i t qui thi nghiem fhly r i n g luan trung II thiic an cd Inh hudng quyet dinh d i n ty le sdng ciia c l ti-ong nhiing ngly diu tien. D l dat dugc ty le sdng cao va sinh trudng tdt trong uong nu6i ca moi nd thi d n cung d p cho c l luan trung voi mat do khoing 5 - 7 con/ml ngay tii ngly diu d i n ngly thii 5, sau do cho c l an Nauplius artemia hoac copepod nhung ngly tilp theo. Nen bd sung tlo tuoi lien tuc trong sudt qua trinh nu6i, cd the sir dung tao kh6 d l thay thi. Viec sii dung thiic an tuoi sdng trong qua trinh uong nu6i tdt hon sir dung thiic an tdng hgp vi thuc an tuoi sdng cung d p cho c l con nhiing nguyen td vi Bing 6. Chilu dli e l Khoang Co
lugng cin thilt ma thu'c an tdng hgp khong co, kich thich bit mdi tdt hon.
2. Anh hudng cua do man din sinh tinrdng v l ty 1^ sdng d
Thi nghiem Inh hudng cua do man len sinh trudng va ty le sdng cua ca tir 15 ngay tudi dugc bo tri voi cac thang do man tfr 5%o den 40%o nhlm muc dich thli nghiem md rong pham vi do man cd the nuoi dugc ca va xem xet qua trinh sinh trudng a cac do man khac, giup ngudi nu6i cham sdc c l tdt hon.
a. Mot sd yen td moi truirng trong thi nghiim Cac ylu td m6i truong trong thi nghiem dugc the hien d b i n g 5.
Bang 5. Cle y l u td mdi trudng trong thi nghiim Nhiet d6
(t"Q 24-28
pH 7,9-8,4
DO (mg/l) 5,5-6,5
NO3 (mg/l) 0-0,05
N H ; (mg/l)
0-0,2
Cac ylu td m6i trudng dugc quin ly tuong ddi dn dinh vl dao dong n l m trong khoang thich hgp cho su phlt triln ciia c l (HI Le Thi Lgc, 2005).
Him lugng DO cd suy gilm sau mdt thoi gian nu6i nhung vin dn dinh d muc cao (DO > 5 mg/L), khi DO gilm thi tien hanh sue khi nen him lugng DO dn dinh hon.
Him lugng nitrat tang len theo thoi gian nu6i do fang qui trinh trao ddi chit cua ca va tang lugng chat thai do ca ldn. Tuy nhien ham lugng nitrat van nlm trong gidi han cho phip.
b. Anh hudng ciia do mdn lin sinh trudng ciia cd Tang tra'dng ciia c l d d c nghiem thuc dugc fhl hien d bing 6.
Dd theo tudi b d e nghiim thiie (mm)
Nghiem thiic 5%o 10%o 15%o 20%«
30%o 35/uo 40%o
Tuoi (ngav) 15
13,2 ±0,30"
13,2 ±0,30"
13,2 ± 0,30''' 13,2 ± 0,30'^
13,2 ± 0,30"
13,2 ±0,30"
13,2+0,30"
25 13,67+0,159"
14,73 ±0,248'' 14,53 ±0,291"
15,73 ± 0,396' 15,73 ±0,284' 15,67 ±0,270'
35
17,67 ±0,287"
18,33 ±0,421'"'' 18,57 ±0,202"
18,75 ±0,281"' 19,53 ±0,256'
45
19,21 ±0,252"
19,07 ±0,241"
19,42 ± 0,248"
19,09 ±0,159"
19,69 ±0,194"
60
22,57 ±0,196'*
21,90 ±0,273""
22,67 ±0,273"
21,80 ±0,301"
22,57 ± 0,298""
Sdlieu trinh bdy trong bdng la gid tri trung binh = sai sd chuan. Sdlieu ciing cot cd cdc chu cdi khde nhau the Men sai khde co y nghia thdng ke (p< 0,05).
Chilu dai trung binh c l Khoang Cd Dd d d c nghiem thiic 30%o, 35%o, 40%o ldn hon so vdi chilu dli trung binh cua c l d cac nghiem thiic 10%o, 15%o vl 20%o d ngly tiidi thu' 25 vl 35 (p < 0,05) (Bang 6). Cl cd tdc do fang trudng cao d cac nghiem thu'c 30%o, 35%o, 40%o d ngay tudi thu 25 vl 35 so vdi d c nghiem fhiic 10%o, 15%o vl 20%o (Bang 7).
Bang 7. Tde d6 tang trudng ehilu dli eua c l Khoang Cd Dd (mm/ngly)
Chi ti6u
Chilu dai
tang (mm/ngay)
D d m a n e/60)
10 15 20 30 35 40
Tudi (ng^v thli) 25
0,04 0,15 0,13 0,25 0,25 0,24
35 0,29 1 0,38 0,28 0,30 ~^
0,38 45 0,15 0,07
[70,08 h-M3_
LIMII
60 0,22 0,18 0,21 0,18 0,19
Qua bing 7 ta thIy tdc do tang tracing chilu dai ciia ca cao d ngay tijdi thii 35 va thIp nhlt d ngay tirdi thu 45, a nghiem thuc 10%o thi tdc d6 tang tiiidng chieu dai rat thap 0,04 mm/ngay do ca thich nghi kem d do man nay, ca tdn nhilu nang lugng de dieu hoa ap suit tham thau nen fang tiirdng cham, 6 ngay tiidi thii 25 thi ca a nghiem thu'c 15%o va 20%o dang ti-ong qua trinh thich nghi nen co tdc do tang tindng cham hon so voi c l d nghiem thiic cd do man cao.
c. Anh hudng cua do man din ty le sdng cua cd (J nghiem thuc 5%, ca 15 ngay tudi chef h i t sau 3 ngay thi nghiem.
6 nghiem thuc 10%o, ca chef holn toln sau 13 ngay nu6i thi nghiem ( d 28 ngay tudi). Tu^ ngay diu thi nghiem den ngay thu' 10 tiii c l chi chef rli rac. Ngay thu 11, ca CO cac bieu hien khIc thudng: ca kh6ng tu din d gdc be, ca boi 16 dd tren mat nuoc, mdt sd c l the nlm ling day, ca trong b l hau nhu bd an va sang ngay thu 12 thi chit hon niia, cac ca fhl con lai rlt ylu va sang ngay thii 13 thi chit toan b6 sd ca cdn lai.
s6ng(%)
,(1).
i?
120 - 100 - 80 60 - 4 0 20 -
0 -1 0 5%o
0
1 0%o
9 2 c )3. 3 9 6
15%o 20%o 307c N g h i e m t h i > c
c )8.
o 357c 7 9 6
0 407oo
sdng cao (> 92%) va co tdc dci tang truong gan nhu nhau, thich hgp nhat la fir 30%o din 40%o. Trong qua trinh nuoi kh6ng nen d l dd man giam xudng duoi 15%o se Inh hucing d i n sue khde ca.
IV. KET LUAN
Kit qui thu nghiem Inh hudng cua thuc an va dci man din sinh tiirdng va ty le sdng cua ca Khoang Co Dd (duoi 60 ngay tijoi) cho thIy, thirc an II tlo tijoi, luan tiling va copepod dong vat bg chan kiem giup ca duoi 30 ngay tudi tang tradng nhanh hon cac loai thuc an khac (p < 0,05), co ty le sdng lon nhlt (75,5%) va ca CO mau sac dep gin gidng vdi ca ngoli tu nhien. Do man thich hop cho uong nu6i ca (tii 15 den 60 ngay tiidi) tu 15%o den 40%o, thich hgp nhlt ti-ong khoang 30 - 40%o va CO ty le song cao Gdioang 96%). Tuy nhien, day chi la nghien cuu khIo sat ban diu nen d n c6 nhung nghien cun sau hon d c yeu td sinh thai den smh tiirdng, ty le sdng vl miu sic cua cl.
TAI UEU THAM KHAG
1. DIo Tan Hd va cong tac vien, 2001. Thanh phln h6i sinh giua HIi Quy va ca Khoang Co a Vinh Nha Trang. Tuyi'n tap bdo cdo khoa hoc Hoi nghi Klioa hoc Bien Dong 2000. Nxb N6ng nghiep TP. HCM.
2. Ha Thi Le Lgc, 2002, Mot sd ddc diem dinh dudng cua cd Klioang Cd (Amphiprion clackii). Bao d o khoa hgc, Vien Hai duong hgc Nha Trang.
Khanh Hoa,
3. HI Thi Le Lcic, 2005. Nghiin cim ca sd sinh thdi, sinh hoc phuc vu cho sinh sdn nhdn tao cd KJwang Cd (Amphiprion sp.) viing bien Kfidnh Hoa.
Luan van tiln si sinh hgc, Vien Hai duong hgc Nha Trang. Khanh Hoa.
4. Woott;on R. J., 1995. Ecology ofteleost Fishes.
Chapman & Hall. 404 p.
Hinh 2. Ty 11 sdng eiia e l b e l e nghiim thiic (%) a cac nghiem thu'c 15%o, 20%o, 30%o, 35%o va 40%o thi ty le sdng cao > 92%. Ca chit trong khoing 10 ngay diu thi nghiem, sau giai doan nly d i n k i t thuc thi nghiem thi ca chit rat it.
Tu' cac kit qui vl ty le sdng va sinh tradng cua ca Khoang Cd Dd d cac d6 man khIc nhau thi ta CQ the nu6i ca d do man tu' 15%o d i n 40%o, c l dat ty le
THE IMPACT OF FEED AND SMJHYTi TQ Amphiprion frenatus GROWTH AND SURVIVAL RATE
Tran Van Phuoc', Nguyen Dmh Trung\
Vo Thanh Dat' Ha Le Thi Loe^
Summary
The shjdy on the effect of feed and salinity on AmpMprion frenatus growth and survival rate was carried out at Nha Trang Institute of Oceanography from March to June, 2009. The results showed that the feed was Nanochoropsis oculata, Brachionus plicatilis and Copepod had a significant effect on the growth survival rate (75.5%) and colour offish (P < 0.05). The salinity for fish growth was from 15 ppt to 40 ppt especially the best growth was from 30 ppt to 40 ppt (survival rate over 96%). However, that was only the first research so to have to research into ecology factors effect on growth, survival rate and colour offish.
Keywords: Effect, salinity, feed, survival rate, growth Amphiprion frenatus jishspeccib.
Ngudi phln bi6n: GS.TS. Mai Dinh Y6n
NONG NGHIEP VA PHAT TRIEN NONG THON - SO 1 - THANG 1/2010 91
