concentration of the total phytosterols was found 0.347% (calculated on the absolute dried herbs).
Tai lieu tham khao
1. V6 Van Chi, Tu- dien cay thuoc Viet Nam, Nxb y^pc, (1997) tr. 250-253.
2. Nguyen Thj Hoai, Ha Thj Xuan Thu, Nguyen Thi Quynh Trang, Huynh Ngpc Dilm Antihyperglycemic effect of Musa seminifera Lour. Rhizome in mice.
Proceedings of The Sixth Indochina Conference on Phanvaceutical Sciences. (2009) 61-63.
3. Bui My Linh; Nghien cdu ba du-pc lieu hu-dng tac dyng dieu trj soi than: Chuoi hpt Kim tien thao - Rau om, Lu$n an Tien STDWQC hgc, (2007), Dgi hpc Y Du-pc TP. H 6 Chi Minh.
4. D 5 QUOC Viet, Nghien cdu thanh phan hoa hpc va hogt tinh sinh hpc cua cay bau dat (Gynura sarmentosa DC), cay cai dong (Grangea maderaspatana Poir.) va qua chuoi hpt (Musa balbisiana Colla), Lu$n an Tien sT Hoa hgc. (2006) Vien Hoa hpc.
5. A. P Kenny, The Determination of Cholesterol by the Liebermenn-Burchard Reaction, Biochemical Joumal, 52,(1952) pp. 611-619.
6. Fathaiya Jamaluddin, Suhaila Mohamed, Md.Nordin Lajis, Hypoglycaemic effect ofParkia speciosa seeds due to the synergistic action of p- sitosterol and stigmasterol. Food Research Intemational, (2010) 43(1), pp. 133-139.
7. S. Panda et al. Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea monosperma. Food Research Intemational, (2010;43(1), pp. 133-139.
8. T.A. Miettinen, P Puska, H. Gylling, H.
Vaniianen, E. Vartiainen , Reduction of serum cholesterol with sitostanol-ester margarine in a mildly hypercholesterolemic population, N. Engl. J. Med., (1995) 16, pp. 1308-1312.
9. WHO (2005), Global and social implications of the diabetesworldwide, Geneva.
10. http://www.dietaryfiberfood.com/cholesterol- low.php (Update 2009)
*? r
Trien khai mo hinh gay co that cff trffn
r IV
phe quan tai cho tren chuot lang va ap dung
r •?
nghien ciru tac dung cua du'O'c lieu xau ho (Mimosa pudica L. Mimosaceae)
Nguyen Quynh Chi', Nguyen Thu HSng', Dinh Dai Do', Dao Thi Vui', Tran The Bach^, Nguyin Hoang Anh' ' Trudng dgi hoc Duac Ha Noi ' Vien Sinh Thai va Tai nguyen sinh vat, Vien Khoa hoc Viet Nam
Dat van de
Theo u-de tinh cua To ehdc Y td thd gidi, nam 2008 trdn t h i gidi cd khoang 300 trieu ngu-di bj hen p h i quan trong dd Ddng Nam Chau A la khu vyc cd do lu-u hanh gia tang nhanh n h i t So vdi cac bdnh ly man tinh khac, ty Id t d vong do hen tu-ong ddi t h i p , tuy nhidn phi ton do hen gay ra lai ddng d mdc cao nhiL bao gdm ca chi phi trye tidp va gidn tidp cho cham sdc va didu trj bdnh '^' Ngoai vide s d dyng hda du-oc didu tri kinh dien nhu- cac thudc kich thich P2 giao cam, cac corticosteroid, theophyllin, cac thuoc doi khang leueotrien,
nhdng nghien cdu g i n day trdn the gidi cho t h i y ty Id bdnh nhan hen s d dyng cac thudc bo tro va thay t h i ngay cang cd xu hu-dng gia tang, trong dd s d dyng d y o c lieu la mdt trong nhdng hinh thdc pho bien n h i t '^'
Oe danh gia tac dyng cua mdt thudc (ke ca thuoc cd ngudn goc du-oc lieu) hu-dng tac dyng dieu trj hen p h i quan, nhieu md hinh du-oc ly da du-oc xay dyng: md hinh trdn co tron phe quan tai chd, trdn eo tron phd quan cd lap, md hinh gay hen theo co che dj dng b i n g ovalbumin '^' '*' Trong khudn kho nghidn cdu nay, chung tdi tidn hanh trien khai md hinh gay co that co tron
TAP CHI DUQC HQC - 12/2011 (SO 428 NAM 51) 41
Nghien CLFU - Ky thuat
phe quan tgi ch6 trdn chudt lang theo phyong phdp cua Konzett - Rossler d i ddnh gid tdc dyng cua du-oc lidu theo hu-dng diiu trj hen p h i quan vd dp dyng md hinh ndy d i nghidn cdu tdc dyng cua du-oc lidu X i u h4 {Mimosa pudica I.
Mimosaceae). OSy Id mdt du-oc lidu moc hoang dai tgi Vidt nam cdn chu-a dyoc nghidn cdu nhiiu v i thdnh phin hda hoe cung nhu- tdc dyng sinh hoc, nhu-ng dd du-oc sd dyng trong mdt s6 nin y hoc c6 truyin d i diiu trj hen p h i quin.
Nguyen vat lieu va phu'O'ng phap nghien cu>u
Nguyen v^t li^u
Dwac li§u nghi§n cwu
Phin trdn m$t dit cua cdy xiu h i {Mimosa pudica L. Mimosaceae) dyoc thu hdi vdo thdng 6/2010 tgi khu vyc ngoai thdnh Hd Ndi vd gidm djnh tdn khoa hoe bdi Trin Thi Bdeh, phdng Thyc vdt, Vidn Sinh thdi vd tdi nguydn sinh vdt. Du-oc lidu du'OC Idm sach, cit nho, phoi, siy khd.
Chuan bj mau thir
Djch chiet nu-dc thu du-oc bing phu-ong phdp sic vdi dung mdi Id nu-dc (dun sdi trong 1 h, Idp lai 3 lin). Gdp djch chiet nu-dc, bay hoi dung mdi den dang cao Idng 1:1. Djch chiit con thu du-oc bing phu-ong phdp chiit hoi lu-u vdi dung mdi ethanol 90° trong 1 h, Idp Igi 3 lin. Bay hoi dung mdi d i n c i n . C i n du-oc hda tan lai vdo nu-dc (ehda CMC 0,5%). Miu thd (djch chiet nu-dc, djch chiit con) du-oc chuan bj vdi lieu phu hop de cho chudt thi nghidm u6ng.
Thuoc, hda chat
Thuoc doi chieu: diphenhydramin cua Cdng ty Du-gc phim VFnh Phuc, aminophyllin cua hang Gedeon Richter (Hunggari), histamin dihydrochlorid cua Biomedicals (Phdp), thiopental cua RotexMedieal (Ode), xylazin cua Keprc) (Hd lan).
Dgng vat thi nghidm
Chudt lang, ca 2 giing, trong lu-ong td 350- 400g, khoe manh do Hoc vidn Qudn Y cung cip. Chudt dyoc nudi 6n djnh trong diiu ki0n phdng thi nghidm 2 tuin tru-dc khi thyc hi$n nghidn cdu, dyp-c nudi dydng bing thdc Sn tidu chuin, nydc u6ng ty do.
IVIay moc, thiet bj, dung cy
Thiit bj nghidn cdu co thit p h i quan g i m 3 bd phdn: mdy thdng khi hd trp- (Rodent Ventilator, model 7025), bO ghi vS khuyich dai tin hidu co thit (Bronchospasm transducer model 7020), mdy ghi d i u dd 1 kdnh (Uni record model 7050) cua hdng Ugo Basile (Y).
42
Phu'O'ng phap nghidn ciru
Triin khai md hinh giy co that phe quin t^i ch8 tr§n chu^t lang theo phipong phdp Ciia Konzett-Rossler
Nguydn tic; Ddp dng co thit p h i quan dyoc ghi dya trdn sy thay d i i t h i tich khdng khi trong dydng hd hip cua ddng vdt thi nghidm dyoc dSt trong 1 hd thing kin g i m 1 mdy thdng khi h6 tro vd 1 6ng mao quan chda nydc cho phdp do the tich ho$c dp lye cua khdng khi du- thda. Khi phi quin bj eo thit du-di tdc dOng cua tdc nhdn gdy co thit nhu- histamin, lu-ong khdng khi phdt ra td m^y tro thd vdo phii giim di, Idm t§ng the tich khdng khi thda ra (phin khdng bj chiim gid bdi phii sau sy CO thit phi quin). Lyong khi thda se tgo ra dp suit Idm ddng edt nydc trong 6ng mao quan cua bd ph$n ghi. Tin hi^u s§ du-oc khuyich dgi vd dyoc ghi trdn bdng giiy cho phdp lu-ong gi^ mdc dd CO thit p h i quan'^
Triin khai md hinh:
+ Chudt du-oc bOc 10 khi quan, ddt ndi khi quan vd thiit l$p thdng khi nhdn tao qua mdy sau khi gdy md bing c^ch tidm phuc mgc hdn hop xylazin (5 mg/kg) v^ thiopental (40 mg/kg).
Do CO thit p h i quan chuOt lang binh thu-dng vd CO thit xay ra khi dung mOt kep nhd de kep chSt khi quan (co thit eye dgi)
+ Khao s^t ddp dng p h i quan vdi t^c nhin gdy CO thit Id histamin: tidm tmh mgch c6 histamin vdi lieu tang d i n td 0.5 den 15 pg/kg (0,5; 1, 2; 4; 6; 8; 10 v i 15 pg/kg). Khoang cdch gida cac l i n tidm Id 2 phut). Mdc dO co thit khi quan chudt du-oc ghi Igi trdn bang giiy lidn tyc sau khi tidm histamin. K i t qua du-oc bieu didn theo ty 1$ phin tram co that so vdi co thit cy-c dgi tgi cac thdi diim 5, 10, 15 vd 20 phut, td dd lya chpn liiu histamin thich hop cho cdc thi nghidm tiip theo'^'
Ap dyng md hinh tr§n de danh gia tac dyng chong co that ca tran khi quan cua djch chiit todn phin vd cdc phan doan djch chiit cSy xau ho
ChuOt thi nghiOm SufQfc chia ngiu nhidn thanh nhiiu 10, mOi 10 6 con. Ap dung mO hinh da dyQ-c trien khai a phin trdn:
LO 1 (10 chdng bOnh): chi dygc dyg tic nhan gay co that phe quan Id histamin.
- LO 2 (10 d i i chiiu diphenhydramin): tidm tTnh mgch diphenhydramin (2 mg/kg).
- LO 3 (10 d6i chiiu aminophyllin): tidm tTnh mgch aminophylin (6 mg/kg).
Ty\P CHI DUQC HQC - 12/2011 (SO 428 NAM 51)
Lo 4 (Id thd uong djch chiet nu-dc cua x i u ho): udng djch chiet nu-dc cua x i u ho (1:1) vdi lidu 5,6 g/kg tinh theo du-oc lidu khd.
Lo 4 (Id thd uong djch chiit con cua x i u ho): udng dich chiit eon cua x i u ho vdi liiu 5,6 g/kg tinh theo du-oc lidu khd.
Sau 15 phut (vdi cac Id doi chieu) vd sau 1,5 gid (vdi cac Id thd uong cdc djch chiit cua x i u ho), tidm tac nhdn gdy eo thit histamin vdi lieu da du-oc lya chon. Ghi ddp dng eo thit lidn tyc trong vdng 20 phut. Kit qua du-oc bieu didn theo ty Id phin trdm eo thit so vdi eo thit eye dai tai cac thdi diim 5, 10, 15 vd 20 phut sau khi tidm histamin. Xdc djnh mdc dd doi khdng eo thit cua cdc thuoc so vdi Id chdng bdnh.
XiF ly s 6 lieu
Sd lidy duoc lu-u trd vd xd ly theo phin mem SPSS 16.0. Tien hdnh kiem chuin m i u tru-dc khi xd ly. Cdc m i u phdn phoi chuin du-gc dai didn bing gid trj .V±SD vd du-oc kiim djnh bing test T-studenL Cdc m i u phdn bo khdng chuin du-gc dai didn bing gia trj trung vi Me, Min va Max dong thdi du-gc kiem djnh bing cdc test phi tham so: Kruskal Wallis Test va Mann-Whitney U Test. Sy khac bidt du-gc coi la cd y nghTa khi p < 0,05. Phan tich lidn quan lidu lu-gng cua histamin vdi dap dng co thit phe quan du-oc thyc hidn theo md hinh khdng tuyen tinh sigmoid trdn phin mdm GraphPad Prism 5.0.
Kdt qua va ban luan
Trien khai mo hinh g i y co t h i t p h i quan tai chd tren chuot lang
•o
c 3 &
ra 150-
120-
90-
60-
30-
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d
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.
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• - - I
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'
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S 1 0 1 5 2C lieu histamin {fuglkg the trong chuot)
Hinh 1: Lien quan liiu Iwgng vd ddp wng co thit phi qudn cua histamin
Md hinh gay eo thit p h i quan tgi cho tren chudt lang cd Idp du-oc trien khai theo md ta cua Konzett va Rossler. Liiu histamin sd dyng trong md hinh ndy md ta trong y van biin thidn rit rdng, td 1 (ig/kg d i n 20 |ig/kg'^' Vdi muc dich
lya chpn lieu histamin phu hop cho md hinh, chung tdi da khao sdt ddp dng co thit p h i quan gdy ra bdi histamin d nhieu mdc liiu khdc nhau tdng d i n td 0,5 den 15 |.ig/kg (hinh 1). Lieu histamin td 2 ng/kg trd Idn dd gdy ddp dng co thit p h i quan rd rdt. Ddp dng tdng d i n khi tdng liiu histamin sd dyng vd dat eye dai vdi lieu cao nhit dd thd (15 i^g/kg), vdi kha ndng gdy co thit phe quan tu-ong du-ong vdi ddp dng eye dai thu du-oc khi kep ch^t khi quan chudt lang. Tinh todn cdc thdng so du-oc lye hoc cua moi lidn quan liiu lygng - tdc dyng theo md hinh khdng tuyen tinh sigmoid (md hinh cd tinh phu hop kha chat, R^ = 0,86) vdi dd lidu thyc nghidm trong hinh 1 cho thiy lieu ED50 cua histamin khoang 5,94 i-ig/kg chudt (bang 1). Vi vdy, chung tdi quyet djnh lya chpn lieu 5 |.ig/kg chudt la lieu tidm tTnh mach cua histamin trong md hinh cho cdc thi nghidm tiep theo. Mdc lieu ndy vda du de tao ra ddp dng co thit phe quan rd ret, vda cho phdp duy tri cdc ehdc ndng sinh tdn cua ddng vat thi nghidm, dac bidt trong didu kidn thdng khi nhan tao va gay me lau khi thd cac thudc doi khang sd dyng qua du-dng uong.
Bang 1: Cdc thdng sd dac trwng md td mdi lien quan liiu Iwgng - tdc dung cua histamin
Thong so (don vi)
Em,n (%) Emax (%)
ED50 (ng/kg) R^
X±SD 32,32 ±6,10 84,67 ± 7,05 5,94 ± 0,44
0,86
Khoang tin cay 95%
16,64-47,99 66,55-102,8 4,82-7,07 Oanh gia tac dung chong co that co tron p h i quan cua djch c h i i t nu'dc va djch chiit cOn tCr du'O'C lieu x i u hO
V.
Histamin (S |ig/kg) Diphenhydramin (2 mg/kg) Aminophyllin (6 mg/kg) Dich chiet nuoc (5,6 g/kg) Dich chiet con (5,6 g/kg)
10 2S
Thol gian (phut)
Hinh 2: Tdc dung cua diphenhydramin, aminophyllin, djch chiit nw&c vd djch chiit cdn tw dwgc lieu xiu hd tren ddp wng co thit phi quan gay ra b&i histamin
TAP CHi DUQC HQC - 12/2011 (SO 428 NAM 51) 43
Nghien
CLFU- Ky thuat
Bang 2: Tdc dung cua diphenhydramin, aminophyllin. djch chiit nw&c vd djch chiit cdn xiu hd trdn ddp wng co thit phi qudn gdy ra b&i histamin
Nhdm
Histamin' Aminophyllin^
Diphenhydramin^
DC nu-dc Xiu ho"
P DC c6n Xiu h6^
P
Median (Min - Max) (%) 0-5 phut 5-10 phut 10-15 phut 95,00(37,90-126,80) 71,60(21,10-100,00) 82,30(19,50-106,40)
12,16(4,00-100,00) 20,39 (4,26-70,00) 13,00(5,88-33,3)
P41 = 0,002 P4 2 = 0,937 P4.3 = 0,699 15,36(8,11 -48,81)
P5.1 = 0,002 Pv4 = 0,589
6,82 (2,63-28,00) 9,07 (4,74-25,00) 17,89 (4,26 -56,00) 20,13 (4,26 -58,33)
10,00(7,33-27,78) 9,41 (7,50-28,12) P4.i= 0,015 P4.i= 0,009 P4.2 = 0,240 P4-2 = 0,394 P4-3 = 0,699 P4-3 = 0.394 12,44(8,11 -16,67) 14,32(8,11 -39,06)
P5-i= 0,002 P5.i= 0,015 P5-4 = 0,699 Psw, = 0,394
15-20 phut 83,25(26,30-106,40)
11,31 (4,21-28,00) 37,42 (4,26-70,00) 10,29(4,00-28,12)
P4-1 = 0,009 P4-2 = 0,937 P4-3 = 0.180 14,08(9,73-59,46)
P5-1 = 0,009 P5-4 = 0,485 Histamin vdi liiu 5 jig/kg cho phdp duy tri ddp
dng eo thit phi quan (> 70% ddp dng eye dgi) trong suit thdi gian theo ddi. Hai thuie doi chdng:
diphenhydramin (thuie khdng thy thi H, cua histamin), tdc nhdn dii khdng ddc hidu vd aminophyllin (thuoc gidn co tron phe quan do Idm tdng hdm lu-ong AMP^, trong te bdo), tdc nhdn dii khang ehdc ndng the hidn rd tdc dyng doi khdng vdi tac dyng co thit eo tron phi quan gdy ra bdi histamin trong suit thdi gian theo ddi (p < 0,05 so vdi Id chdng histamin). Djch chiit cin vd djch chiit nu-dc cua xiu ho cung cd tdc dyng de ehi rit manh dap dng eo thit phi quan gdy ra bdi histamin (p < 0,05 so vdi Id chdng histamin) trong suit thdi gian theo ddi. Tdc dyng cua djch chiet nu-dc vd djch chiit cdn khdng khac nhau cd y nghTa (p > 0,05) vd tu-ong du-ong vdi tdc dyng doi khdng cua diphenhydramin vd aminophyllin (p > 0,05, so sdnh djch chiet nu-dc hode d|eh chiit eon vdi diphenhydramin vd aminophyllin). Kit qua ndy cung phu hop vdi kit qua cdng bo gin ddy ddnh gid tdc dyng hu-dng dieu trj hen phi quan thy-c nghidm cua du-oc lidu xiu ho trdn cdc md hinh khde du-oc thyc hidn bdi nhdm tdc gia Hdn qu6c vd An Od. Prabha va cdng sy d§ ghi nhdn tdc dyng bao v$ cua djch chiit nydc xiu ho trdn md hinh gdy co gi$t do qud min vdi histamin vd tdc dyng de ehi sy vd hgt cua t i t)ao mast trdn in vitro cua djch chiet ndy"^ Djch chiit con td bd phdn trdn mdt dit cua xiu h i cd tdc dyng chong vidm mgn tinh trdn md hinh gdy hen dj dng bing ovalbumin thdng qua vide de c h i sy di chuyin cua bgch ciu, dc chi sy giai phdng cdc cytokin gdy vidm (IL-6) td cdc t i bdo hidu dng, giam so lu-ong bgch ciu, bgch ciu u-a eosin, giam ham lu-ong IgE trong dich tiet phi quan '^' Tdc dyng doi khdng co thit eo tron phi quan ghi nhdn trong nghidn cdu cua chung tdi cung vdi cdc tic dyng chdng vidm trdn md hinh hen mgn tinh thyc
nghidm dS gdp phin tao co sd cho vide phdt then xiu h i thdnh du-p-c lidu cd tiim ndng trong diiu trj hen phi quin.
Kdt luan
Chung tdi dd trien khai thdnh cdng md hinh gdy CO thit CO tron phi quan tgi cho trdn chudt lang de ddnh gid tdc dyng cua thuoc (ho$c du-oc lidu) theo hu-dng dieu trj hen phi quan. Lieu 5 ng/kg histamin Idm tdng cd y nghTa ddp dng co thit vd duy tri ddp dng ndy trong suit thdi gian theo ddi.
Djch chiit nu-dc vd dich chiit con cua xiu ho vdi liiu 5,6 g/kg cd tdc dyng dc che ddp dng co thit phi quan trdn md hinh. Tdc dyng dc che cua djch chiit nu-dc vd djch chiet c i n cua xiu ho tirong du-ong vdi tdc dyng cua diphenhydramin (lieu 2 mg/kg) vd aminophyllin (lieu 6 mg/kg).
Summary
7776 standard procedure of respiratory phanvacology for evaluating bronchospasmolytic activity in anesthetized guinea pigs (modified Konzett-Rosseler's) which had not been applied extensively in Vietnam to evaluate bn:)nchospasmolytic effects of medicinal plants was used in this study to estimate the bnxhospasmolytic effects of the aqueous and ethanol extracts of Mimosa pudica L, with histamine (5^ig/kg, IV) as spasmogen. The plant aqueous and ethanol extracts exhibited the desirable effect at dose of 5.6 g/kg, po, significantly reducing bronchospasm as compared to the control This bronchospasmolytic activity was comparable to that of diphenhydramine (2 mg/kg, IV) and aminophyllin (6 mg/kg, IV). Further evaluations should be required for this promising medicinal plant in treating asthma.
(Xem tiep trang 56)
44 TAP CHI DUQC HQC - 12/2011 (SO 428 NAM 51)
Yeu to dd i m cd t i e ddng den c h i t luong thuoc Idn hon so vdi yeu t i nhidt dO trong q u i trinh bao quan thuoc, d i e bidt vdi c i c dang c h i p h i m de hut i m nhu- vidn bao hay nang
D i y l i ly g i i i hop ly cho vi$c phdt hidn nhiiu m i u thuic khdng dgt chat lu-png n h i t l i thuic nhgy cam vdi mdi tru-dng khi bao quan tai c i c vung ven bien hay vung nui l i nhu-ng noi cd d i i u kidn khi hdu k h i c nghidt Cung dong thdi c i n tdng eu-dng cdng t i e kiem tra v i g i i m s i t c h i t luo'ng thudc cua c i c co quan q u i n ly trdn c i c d|a b i n vung cao v i ven bien, nhat l i c i c k h i n g sinh va vitamin nhay cam vdi mdi tru-dng d i thu-dng xuyen nhie nhd v i nSng cao cdng t i e dam bao c h i t luong thuoc t i t den tay ngudi bdnh.
Summary
A preliminary research about the effect of drug quality through period of store by temperature and humidity at different geographic areas was earned on three kinds of pharmaceuticals including Aspirin pH8 entenc-coated tablets, Vitamin C film-coated tablets and Amoxicillin capsules of different companies preserved at Thuan An coastal area, Nam Dong mountainous region, flat country of Hue Province and Hue Drug Quality ContnDl Centre. Result showed a considerable decrease of drug quality affected not only by high temperature and humidity in stored period at different areas but also by the stability of each active compound and each produced formula in one kind of different
drugs. The decrease of drug quality happened earliest and strongest at Thuan An, and then Nam Dong, where were sustained a severe climate condition yearly. Especially, effect of humidity factor on the drug decomposition was more serious than temperature. Almost of drugs stored at these areas was less quality after three to five months, presented clearly by the change of physiochemical properties such as appearance and active compound concentration. The decrease of drug quality was also happened at Hue city but in gentle degree, while all drugs were still in good quality under the standard store condition at Hue Drug Quality Control Centre (2^C. 60%) This research showed an importance of the temperature and humidity control during the store and delivery periods to maintain the drug quality sufficiently for treatment.
Tai lieu tham khao
1 Bp Y le. Duoc dien Viet Nam III, NXB Y hpc.
2002
2 Bp Y te. Quyet dmh ve viec ban hdnh nguyen tdc. tieu chuan GPP 2007
3. Bdo cdo tong kit cdng tac kiem tra lay miu kiem nghiem chat lu-png thuoc cua Trung tam kiem nghiem TT-Hui ndm 2005, 2006, 2007, 2008, 2009.
4 Dgng Vdn Gidp. Bdi hpc vi tinh dutpc. trie nghiem gia thiet trong nghien cdu khoa hpc, Truong Dai hgc Y ditgc thanh pho Hd Chi '/n-i 2003. tr. 19 - 26
5. Nguyin Thanh, Dia chi Thda Thien Hue, NXB Khoa hQC xa hdi. 2005. tr 42 - 43
T r i e n k h a i . . . (Tiip theo trang 44)
Tai lieu tham khao
1. Bahadori K., Doyle-Waters M. M.. Mgrrg C. et al , Economic burden of asthma: a systematic review, BMC Pulm Med, (2009), 19 (9), 24.
2. Arnold E., Clark C.E., Lgsserson T.J., Wu T., Herbal interventions for chronic asthmg in aldults and children, Cochrane Database of Systematic Reviews, CD005989, (2008).
3. Rieeiardolo F Nijkamp F., De Rose V., Folkerts G.,. The guinea pig as an animal model for asthma.
Curr Dnig Targets. (2008), 9, 452-465.
4. Phgm Xudn Sinh, Nguyin Mgnh Tuyen, Odo Thi Vui, Tdc dyng eCig coc mdn. Id hen. Id cd dpc du'pc tren khi quan chupt lang eo Igp, Tap chi Dwgc lieu. (2010),s6 2,tr 105-109.
5. Vogel G.H., Dmg discovery and development:
Pharmacological assays. 2 ^ edition. Springer-Verlag Berlin. (2002), pp 359-361.
6. Park KM, Park J., Koh D., Lim Y. Effect of saiko-saponin A, a thterpenoid glycosid, isolated from Bupleumm falcatum on experimental allergic asthma, Phytother Res.. (2002). 16: 359-363.
7. Prabha M., Chandrashekhar P., Sheikh R. et al.
Studies on antiasthmatic activity of aquaous extract of roots of Mimosa pudica Linn. Int. Res. J. Pharm., (2011), 2, 104-110.
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