KHOA HOC CONG N G H |
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B I O R E A C T O R C H I M I X I G A P C A C H Q L I A I X I G D E I \ I
SIIXIH TRLTOniG QUAIXIG T U DUOIMG C A Y HOIXIG {Rautawnia fartuneii II\1 VITRO
Nguyin Diic Minh HiingS D6 Thi TuylnS i H n Vdn Minh^
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He thdng nuoi cdy ngap chim tam thdi (temporary immersion system TIS") la ra§t he thdng dang dirpc ap dung khdng nhimg t ^ dung dupe cac uu dilm ciia nuoi dry long. Cac do?in than cSy cdy m6 co chiia 2 mdt mdm doi xiing diroc nu6i cay trong he thdng bioreactor voi 4 nghidm thiic den lED theo ty le xanh vd do la:
10% LED xanh + 90% 1£D do; 15% LED xanh + 85% LED do. 20% LED xanh + 80% LED do; 30% LED xanh + 70% LED do dupe so sanh vdi ddi chiing la cay dupe nuoi cay duoi anh s^g huynh quang thOng thudng.
Cay hong khdng phat trien dupe trdn moi trucmg khong duong v6i mat djnh thdp nhat cua he thdng Id sue khi 1 phiit 1 lan/gi6f. Cay phdt tnen chSm va la bi vdng do kh6ng du COj cho quang hpp. Khi co bd sung khi COj voi ty Id 10% lugng khi thdi vao h§ thdng TIS; chdi va cay hong phdt trien tdt nhat o nghidm thiic chilu sang la 20% LED xanh + 80% LED do. Tuy nhifin vAc b6 sung khi COj gay phiic tap va tdn kem trong viec nudi cay. De khac phuc, vide dimg vi mach cai tiln tang sd Idn ng^p chim cua hd thdng TIS Ifin 2, 3, 4 lan/gid duQC su dung trong Ihi nghidm da cho thdy d nghidm thiic ng|p chim 2 Idn/gid vk chilu sang bdng 20% LED xanh + 80% LED dd cho ciy phat trien tdt nhat, ckc nghidm tiniR: ngap chim 3, 4 Idn/gid cay co hien tuong ling cac la duoi gdc do b| nggp nhilu trong thoi gian dau. Ket qua nghien ciiu cho thay c6 the diing hd thdng TIS cai tiln dl nuoi cay quang tu duong c4y hdng v^ sir dung ngudn sang 20% LED xanh + 80% LED do la tdt nhdt cho loai cay nay.
TvMbiba: Binh phan mg sinh hoc, ddt dian, den LED, cay ciy md, TiS^diimngip each quang.
1.
Hidn nay, cae trudng dai hpc, vien nghien cihi trong nude dang phdt trien nhiing ky thuat mdi nhu:
ky thuat nuoi cay quang tii duong, bioreactor,... d l nhan gidng hoa lan, cdy trdng nong nghiep, lam nghidp, duoe lidu...(Pack et al., 2005) [15]. He thdng nudi cdy ngdp chim edeh quang (Temporary Immersion System TISO Id mdt hd thdng bude dau duoe dp dung khdng nhiing tdn dung dupe cdc uu diem ciia nuoi cay long vk nudi cdy trdn thach ma edn hgn eh^ dupe nhupe diem eda hai hd thdng nudi cdy trdn giiip tao ra mdi trudng nudi cay thoang khi, cdy con khoe manh, ti Id sdng sot eao, giam chi phi nhan cdng, ti^t Idem vd giam chi phi moi tnrdng nudi cay do su dung it mdi trudng trdn mdt mdu cay va khdng su dung thach, hd sd nhdn dupe gia tdng nhi^u Idn so vdi khi nhdh gidng trdn he thdng nudi cdy tiidng tiiudng (PreU, 1991) [16].
Trdn tii^ gidi den LED (vi^t tdt cua light Emitting Diode, cd nghia la didt phdt quang) it tda
'Vien Sinh hoc Nhi?t ddi, YHLKH&CNVN
^'nrdngD^i hoc Qu6c t§, DHQG TPHCM
nhidt vd it tdn kem didn nang da ed rdt nhi^u ling dung thuc tidn. Mdt sd nude nhu Nhdt, My, Trung Qudc, Hdn Qudc cung da vd dang su dung ddn LED xanh vd do vdi nhi^u ^ Id dg ehieu sdng eho tfeig giai doan phdt tri^n cua thue vdt in vitro (Kang et al, 2013) [6]. Dudi dnh sdng den LED, sinh trudng in vitro cua nhidu lodi cdy trdng dd dupe cai thidn nhir.
ddu tdy (Kim et al., 2011) [7], hoa lan vu nir (Mengxi et al., 2011) [10], Jatropha curcas (Norhayati Daud, 2013) [12],taochlorella (Choietal., 2013) [1].
Cdy hdng thupc hp Bignoniaeeae cd ngudn gdc bdn dia Dong Nam A vd dupe di thyc ddn chdu Au vd Bdc vd Nam My. Cd cdc lodi trdng trot chu yeu P.
fortunei, P. kawakamii. P. taiwaniana va P.
tomentosa, trong do P. fortunei co gid tri kinh t^ cao nen dupe phd bien trdng riing phdng hd ddu ngudn (Burger, 1989) [3]. P. fortunei ^dM khi trdng 3 nam ed thd khai thac gd 1dm cdc loai giay cao cdp nhu giay ta ti^n, 1dm than hoat tinh, lam van ep; sau trdng 9 nam se cho gd quy hon gd pomu.
Cdy hdng dupe nudi cay tdi smh tii la (Rao et al.
1996) [18] hay tdi sinh phoi tive tiep 0pekci &
Gozukirmizi, 2003) [4] vd gidn tiep (Ipekei &
NONG NGHIEP VA PHAT TRIEN NONG T H 6 N - KY 2 - THANG 9/2015 57
KHOA HOC C d N G N G H l
Gozukumizi, 2004) [5] nham muc tidu nhan gidng (Hermann & Whetten, 1998) [2]. Chdt luong anh sang ddn fStefano & Eosano, 2003) [19], su tiao ddi khi (Quynh et al., 2000) [17] vd nude (Khan et al., 2003) [8] vd ngudn dnh sang den LED xanh va do (Nguydn Diic Minh Himg & Trdn Van Minh, 2014) [14] dnh hirdng d^n qud trinh nhan gidng tu du&ng vd quang tu duong cdy hdng in vitro.
Do dd, su cdn thidt nghidn ciiu kdt hpp nudi cay cdy hdng in vitro vdi phuong phap quang tu dudng bang hd thdng TIS bioreactor vd chidu sdng bdng den LED nhdm dem lai su phdt tri^n tdt hon cho cdy nuoi cdy va Id co sd khoa hpc eho cdc nghien ciiu ling dung mdi trong nuoi cdy mo td bdo thuc vdt
2 . NGUVBV UEU VA PHUONG PHAP 2.L Vdt lidu
VStUdu cay: chdi cdy hdng paulownia) in vitro.
Mdi trudng nudi cdy mdi trudng Murashige- Skoog [11] khdng bd sung chdt kich tiiich smh trudng, khii tning mdi tnrdng nudi cdy b 121°C, 1 at trong 25 phiit.
Bi^ Mdn nudi cdy: Phdng sdng cd nhidt dd 26±2'*C, am do Rh = 65%, cudng dp chidu sdng ddn huynh quang trdng 50 vunol/m^/s, thai gian chidu sang 12 gid/ngdy.
Ddn LED dupe su dung trong thi nghi§m gdm 2 loai LED vudng 50 x 50 ciia Taiwan sdn xudt, 2 loai den LED cd mdu dd bude sdng 640 - 660 nm vd den mdu xanh duong cd bude sdng 451 - 460 nm, cudng dp chidu sdng ddn LED kdt hpp xanh vd do nhieu ty Id khdc nhau cung la 50 pmol/mVs
Ddn LED 50 X 501^ Id 8036 dd + 2096 xanh Cdc till nghiem dupe nuoi cdy tidn hd tiidng bioreactor bdn ngdp chun Plantuna do Ddi Loan san xuat. Luang khi mdi lan bom vdo binh cdy dupe hieu chinh Id 1 lit/phiit.
Mach dieu khien bdng bd dem digital tirong tir nhu bd cdng tdc ddng cdt ngudn theo thai gian dinh sdn (timer) tinh bdng gidy. Mach cd tiid di^u khiln
cung mpt liic nhidu mdy vdi tiidi gian chay khac nhau vd dieu khidn thdi gian ngdp each quang 1^
phan mdm QEE_STARLED dugrc cung cdp bdi cdng ty LED Anh Sdng Vidt
2.2 Thiet kd thi nghidm
Thi nghidm 1: Nudi cSy hdng hoan chinh bang bioreactor bin ngip chim trdn mdi trudng khdng dudng so sanh vdi mdi trudng cd dudng: 30 doan thdn cdy hdng cay md cd chiia 2 mdt mdm dupe nufli cay tiong 1 binh bioreactor nggp chim cdch quang Plantima cd chiia 300 ml mdi trudng khong duong so sanh vdi moi tiirdng cd dudng binh thudng vd chi^u sdng bdng den h u ^ h quang, thdi gian ngdp Id 1 phiit vd thdi gian cdch quang Id 1 gid-. Ghi nhdn ket qua sau 5 tudn nudi cdy.
Thi nghidm 2: So sinh nudi cay hdng hoin chlah dudi ddn LED trdn mdi trudng khdng dudng cd bd sung CO2: Thi nghidm ndy duge bd tri tirong tu nhu thi nghiem 1, cdy dupe nudi trdn mdi trudng khong dudng cd bd sung Idii COa ^ le 1/10 lupng khi bora vdo (0,1 lit khi CO2 / I Ht khi bam vdo mpt phut cho mdi binh nuoi cdy). Thi nghidm gdm 5 nghidm thiic ehieu sang bang den LED dd vd xanh vdi cdc ty 1^
khac nhau 90/10, 85/15, 80/20, 70/30 vd chidu sang bang den huynh quang thong thudng 1dm ddi chiing de xac dinh lai ty le den LED da thi nghidm trudc day (Nguydn Diie Mmh Hiing vd cs, 2013) [13]. Do dac ket qua thi nghidm sau 5 tudn nudi cay.
Thi nghidm 3: Nudi cay hdng hoan chinh dam ddn LED 20% xanh va 80% do vi den so sinh vdi den huynh quang trdn mdi tnrdng khdng dudng. This gian ngap each quang la 2, 3. 4 lin/gid: 30 dogn tfian cdy hdng cay md cd chiia 2 mat mdm ddi xiing duoe nudi cay trong mpt binh bioreactor n g ^ chim each quang Plantima ed ehiia 300 ml mdi trudng nudi dy khdng dudng. Thi nghidm gdm 3 lan lap l?i bd tri ngdu nhidn vdi nghidm thiic ehieu sdng bdng den LED tiieo ty Id dd vd xanh la: 80% dd + 20% xanh d^t tiong ddn sdng da dupe each ly vdi anh sang bfin ngodi, ddi chiing Id cay duge nudi cay trong he thdng bioreactor ngdp chim cdch quang duoi dnh sang huynh quang thdng thudng. Cdc nghidm thiic diroc hieu ehuih vdi thdi gian each quang Id 15, 20 vd 30 gidy (hrong duang 1, 2, 3 Ian ngdp /gid) va thdi gian ngdp Id 1 phiit.
3.KErQUiiVlilHA0LUAN
Thi nghidm 1: So sdnh nudi cdy hdng hoin 58 NONG NGHIEP VA PHAT TRIEN N O N G THON - KY 2 - THANG 9/2015
KHOA HOC CONG NGH|
chinh b ^ bioreactor bdn n g ^ chim trdn mdi trudng khdng dudng
Do he thdng ngdp chim cdch quang bom khong khi vdo binh nudi mdi gid' 1 Idn vd thdi trong 1 phiit, cd su thay ddi khdng khi bdn tiong binh nudi c^
nen da tiiii nghidm nudi trdn mdi trudng khong dudng de cdy quang hgrp nhu trong tu nhien. Tuy nhidn khdng thu dupe k i t qua nhu mong mudn, ed le do thdi gian cung cdp khi qud ngan (1 phiit/l gia khong ngdp) ndn cdy khong du COa cho quang hpp.
La cdy bi nhat mdu vd phdt triln ehdm so vdi ddi chiing cd duong ndn eac thi nghidm sau sd tiiii bd sung khi CO2.
Bdng 1. Rgt qud tfai nghidm so sdnh nudi cdy hdng
I h i n g h i ^ 2: So sdnh nudi cdy hdng hodn chinh dudi d ^ LED trdn mdi trudng khdng dudng cd bd sung CO2
Sau khi cdi tao bd sung khi CO2 vac ludng khi thdi vdo binh. Sau 5 tudn nudi cay cdy hdng tidn mdi trudng khdng dirdng cd bd sung khi CO2 (10 % lupng khi thdi vdo binh) nudi cdy dudi su chidu sang ctia den LED dd vd xanh vdi ty Id khdc nhau thu dupe kdt qua qua bang 1, hinh 1. Do dae hdm lugng clorophyl tdng sd tiong la cac nghidm thuc thi nghidm tidn, thu dupe cdc ket qua d' bdng 2.
trdn mdi trudng khdng dudng cd bd sung khi CO;
Nghiem thuc Q6i chiing NT 1 (1(WX:9096D) NT 2 (15%X:85%D) NTS (2(MfX:8(»a)) NT 4 (3096X;70%D)
CV(59
Sola 5,5 4,8 6,5 6,6 5,2 17,32
Chii\i cao cay (cm)
3,3 3,0 3,3 4,3 3,1 16,04
S6ri 7,9 5,9 6,2 6,6 5,8 21,96
ehieu dai r^ (cm)
2,4 5,8 5,3 6,1 5,9 23,90
Khdilu^mg tuoi (g)
0,54 0,48 0,63 0,64 0,59 30,71 Bang 2. K^t qud phdn tich clorophyl tdng sd Id cdy hdng nudi cdy dudi ddn LED tidn mdi trudng khdng
dudng cd bd sung CO2
Hinh 1: I h i n g h i ^ cdy hdng trdn bioreactor (1) Tr^n mdi trudng khdng dudng (2) Mdi trudng cd
dudng binh thudng
Cdc k i t qua thi nghidm tien bang 2 cho thay cdy nudi tidn mPi trudng khong dudng d nghidm thiic 3 (dnh sdng den LED 2056 mdu xanh + 80% mdu do) Id tdt nhdt, sd lugng clorophyl ciing eao hon cdc nghidm thiic cdn l^i ke ca ddi chiing. Kdt qua ndy khdc vdi k i t qud nudi cdy tidn mdi trudng cd duong a tidn Id nghidm thiic 2 tdt nhat nhung chi ed khdc biet cd y nghia ddi vdi ehieu cao cdy, cdc chi tidu edn lai khdng khdc bidt cd y nghia thdng kd cd thi do lupng khi CO2 cung cdp chua ddy dii cho quang hop.
Tuy nhidn k i t qud ndy cung cho thay bioreactor n ^ p chim cdch quang cd till nudi cay cdy tien mdi trudng khdng dudng vdi su bd sung COj thich hpp.
Nghidm thiic Ddi chiing NTl(10Wt90%D) NT 2 (15Wt85%B) NT3(209Bt80ffi) NT4(30«X:70%D)
C (mg/ml) 3,955 3,906 4,011 4,397 3,200
Hinh 2: K^t qud thi n g h i ^ so sdnh nudi cdy hdng trdn mdi trudng khdng dudng ed bd sung khi CO2 (a) Bdi chiing, inh sAng ddn huynh quang; (b) Anh sing den LED 10% miu xanh + 90% mau do; (c) Anh sing ddn LED 15% mau xanh + 85% miu do; (d) Anh sang ddn LED 20% mau xanh + 80% miu do; (e) Anh sing den LED 30%mau xanh + 70% miu do.
NONG NGHllP VA PHAT TRIEN N 6 N G THON - KY 2 - THANG 9/2015 59
KHOA HOC CONG N6Ht
Tlii nghidm 3: Nudi cSy hdng hoin chinh du6i stag bang din LED ty 16 xanh 20% va do 80% so sWi d t a LED 20« xanh v4 SOX d6 v4 d4n so sinh v6i tin vtii den h u M Quang trayin thdng, vdi sd Mn ngap h u ^ quang trta mdi trudng khdng dudng (thdi each quang la 2, 3, 4 Mn /gid, mdi Mn ngap 1 phiifll gian ngjp c4ch quang la 2,3,4 lln/gid) So dac chi^u cao cay, sd 14, sd rd, chiiu dai rS, kMl Sau 5 tuin nuoi ciy cay hdng trdn moi trudng ra 1>^« tuoi vi khd ta thu duoe c4c kdt qua d bang ^ rd bdng hd tiidng bioreachir ngdp cdch quang chilu
Bdng 3. Kit qud tiii nghidm so sdnh chilu cao, sd Id, sd rl, c h i ^ ddi r§ ciia cdy Paulownia ti^ mdi trudng khdng dudng chi6i sdng bdng ddn LED so vdi dfai hu^nh quang
Nghidm thiic Sd Idn/gid 2L (Ngap 2 lan, LED) 3L (Ngap 3 lan, LED) 4L (Ngap 4 lan, LED 2H (Ngap 2 lan, HQ) 3H(Ngap31an,HQ) 4H(Ngap41an,HQ)
CV(%I
Chieu cao cay
(cm) 5,100a 3,641b 3,545b 3,326b 3,246b 2,548c 5,85
Sdla
8,30a 6,71b 6,44b 6,53b 6,97b 4,51c 6,36
Sdrl
3,35a 2,84ab 2,35b 2,75ab 2,15b 3,33a 9,29
Chidu daird (cm) l,34ab l.llab 1,01b l,25ab 1,06b 1,45a 15,32
Khdi luong tuoi (g/cay)
0,601a 0,564a 0,473bc 0,539ab 0,405c 0,407c 5,85
Khdi lu^mg khd (g/cay)
0,029 0,026 0,022 0,026 0,019 0,019 6,36
Tyld khd/tuoi
(%)
4,84 4,64 4,56 4,90 4,63 4,60 9,29 Quan sdt cdc binh thi nghidm khi do dac, nhdn
thdy phdn Idn cdc binh cay hdng d cdc nghidm thiic ngap 3 vd 4 Idn/gid cdy cd hidn tugng ling Id dudi gdc d- ca 2 phuong thiic chilu sang bang ddn LED vd den h u ^ h quang, hidn tirong ndy cd thi do gdc cdy bi ngdp trong moi trudng tiong thdi gian dai cay chua thich nghi dugc.
Cac kit qua thi nghiem tidn bdng 3 vd 4 cho thdy cdy nuoi h-en moi trudng khdng dudng b nghidm thiic chilu sdng den LED 20% mdu xanh + 80% mdu dd tdt hon chieu sdng bdng den huynh quang hrong tu nhu d cdy Spatiiiphyllum [9]. Cf cac cap ddi thi nghidm cd thdi gian ngdp nhu nhau, nghidm thiie 2L chieu sang bdng den LED vd ngdp chim 2 Idn/gid smh trudng vd phat triln cao ban nghiem thiic 2H chilu sang bdng ddn huynh quang vd ngdp chim 2 Idn/gid cOng cao hon cac nghidm thtic khdc eho thay cdy dupe chilu sdng bdng den LED vdi su phdi hpp thich hop giiia mdu xanh vd mdu do cho cdy phdt tiiln tdt hon. 0 cdc nghidm tinic 3 vd 4 lan n g ^ /gid, cdy cd till do bi ung tiong thdi gian dau nuoi cay ndn cac chi tidu sinh trudng vd phat triln kdm hon vd ngam nude nhilu hon cdc nghidm thiic cd sd lan ngap 2 lan/gid (ty Id khdi lupng kho/hroi thap hon). Sy khdc bidt ndy cd Jr nghia b ckc chi tidu sd Id, chieu cao cdy vd khdi lupng tiroi khi phan tich tiidng ke. Chi tidu sd rd, chilu ddi rd cua nghiem thiie 2L cung tdt hon cdc nghidm tiuic
khdc vd tuong dirong vdi nghidm thiie 4H Bang 4 ciing eho thdy hdm lupng clorophyl tdng sd tiong Id ciia den LED cung cao hon so vdi nghidm thiic chilu sang bdng den huynh quang ddn din kha nang quang hpp eiia cdy tiong dilu Iddn dung den LED tdt hon. Nghidm thiic 2L cd ham lugng clorophyl Id tdt nhdt (Hinh 3).
Bang 4. Kit qud Ihi n g h i ^ so sdnh hdm tupng cbropl^ cua c ^ hdng trdn mdi trudng khdng dudng
chi&i sdng bdng d ^ l E D so vdi ddn h u ^ q u a p g Nghidm thiic
2L (Ngdp 2 Idn, LED) 3L (Ngdp 3 lan, LED) 4L (Ngap 4 lan, LED 2H(Ngap21an,HQ) 3H(Ngap31an,HQ) 4H(Ngdp41dn.HQ)
Ham luong Clorophyl (mg/gld)
Nhu vjiy cd till su dung bioreactor ngdp chim cdch quang cd thi nuoi cay cdy Paulovraia tidn moi tnrdng khdng dudng vdi su bd sung khdng khi tiiich hpp bang su nit ngdn thdi gian cdch quang, tdng s^
Idn ngap Idn 2 lan/gid Id tdt nhat Su dung den LED vdi ty Id thich hpp cung cho kit qua cay sinh trudng tdt hon den hujnh quang, dieu ndy tuong tu nhu cdc tiii nghidm trudc ddy (Nguydn Diie Minh Hiing &
Trdn Van Minh, 2014) [14].
60 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2015 N6N.
KHOA HOC CdNG N G H |
^lSlbdb^&2i.
Hinh 3: Thi nghidm so sdnh cdy hdng in raCrotrdn mdi trudng khdng dudng bdng ddn LED vd h u ^
quang vdi s6 Idn n g ^ cdch quang 2,3,4 i W g i d (21^ 31^ 4L) Nghidm thuc thi nghidm chidu sing bang ddn LED 20% miu xanh + 80% mau dd, sd lan ng$p2,3. 4 lan/gi&va ngap 1 phiit mdi lin.
(2H. 3H, 4H) Nghiem thiic chidu s^g bing den huynh quang, sd lin ngap 2, 3, 4 lan/gid va ng$p 1 phiit mdi lin.
4.KErUlAN
Cay hdng cd till sinh trudng vd phdt triln tdt tiong dieu kidn nudi cay md d mdi trudng khdng dudng trdn hd thdng bioreactor bdn ngdp chim, chieu sdng bang den LED hode den hu^nh quang truyin thdng vd ed bd sung Mii CO2 hoae tang sd lan ngap chim so vdi mac dinh cua mdy.
Cdy hdng dupe chieu sdng bdng ddn LED vdi ty Id 20% xanh + 80% dd phdt triln tdt hon ddi chiing den huynh quang tidn bioreactor ngdp chim cdch quang tiong dilu kidn mdi trudng khdng dudng vd dimg phdn mim dilu khien tdng sd lan ngap chim Idn gdp 2, 3, 4 lan so vdi mac dinh cua mdy nhdm bd sung khi C02 eho cay quang hpp. Ddi vdi cdy hdng sd lan ngdp chim Id 2 Idn/gid thdi gian ngdp Id 1 phut cho k i t qud tdt nhdt, ngdp chim hon 2 Idn/gid cdy ed hidn tugng ling nude, cdy bi vdng Id gdc.
TMlCUTHAMKlUO
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22:16-24.
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Plant Cell, Tissue and Organ Culhwe 79:341-345.
6. Kang J. H., Kumar S. K., Atiilba S. L S., Jeong B. R., Hwang S. J. (2013). Light intensity and photoperiod influence the growth and development of hydroponically grown leaf lettuce in a closed-^e plant factory system. Hort. Environ. Biotechnol.
54(6):501-509.
7. Kim B. S., Lee H. 0., Kim J. Y., Kwon K. H., Cha H. S., Kim J. H. (2011). An effect of Light Emitting Diode (LED) irradiation tieatment on the amplification of fimctional components of immature strawberry. Hort Environ. Biotechnol. 52(l):35-39.
8. Khan P. S. S., Kozai T , Nguyen Q. T , Kubota C, Dhawan V. (2003). Growth and water relations of Paulownia fortunei under photomixotiophic and photoautotiophic conditions. Biologia Plantarum 46(2): 161-166.
9. Ldpez-Juez E., Devlin P. F. (2008). Light and the control of plant growth. In: Bogre L. and G.
Beemster G (eds) Plant Growth Signaling, pp 233- 242, Springer.
10. Mengxi L, Zhigang X., Yang Y., Yijie F.
(2011). Effects of different spectial Ughts on Oncidiura PLBs induction, proliferation, and plant regeneration. Plant Cell Tiss Organ Cult, 106:1-10.
11. Murashige T , Skoog F. (1962). A revised medium for rapid grovrth and bioassays with tobacco cultures. Physiol. Plant, 15:473-497.
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STUDY OF THE EFFECTS OF I£D-UGHTING AND TI&BIOREACTOR ON AUTOPHOTOTROPHIC GROWTH OF HONG {Paulownia fortuneO IN VTTRO
Nguyen Due Minh Hung\ Do Thi TuyenS TVan Van Muih^
^Institute of Tropical Biology VAST^ international University VNU-HCM.
Summary
Temporary Immersion System TIS' was apphed to manipulate the adventages of liquid cultures. Intemode cutting of Paulowma including two nodes were cultivated in TIS-bioreactor with 4 levels of I£D-light colour combinations in tiie ratio of red-blue LED-lighting as: 1096 blue-LED + 90% red-LED; 15% blue-LED + 8596 red-LED; 20% blue-LED + 80% red-LED; 30% blue-LED + 70% red-LED in comparision with fluorescence light f^ulownia plantiete were wick development in the midia culture with sucrose free and combined with the gas flowing 1 minute/1 time/hour. The plantiets were growth slowly and leaf changed to yellow. It was supplemented with COg-gas by 10% total-gas flowed into TIS-bioreactor. The planlets were growth and development better in the combination of ratio LED-lighting colors by 20% blue-LED + 80% red-LED.
However, adding COj were high charge and difficult to control. The resolution was using IC to set up the immersion programs in TIS-bioreactor to 2, 3, 4 time per hour. It was shown that immersion of 2 time/hour under U:D-lighting by 20% blue-LED + 80% red-UED gave plandets having die best growdi and development.
Keywords: Bioreactor, intemodes, LED, piantlets, TiS-temporary immersion system.
Ngudi phdn bidn: PGS.TS. Nguydn Van Ddng Ngdy nhdn bdi: 24/4/2015
Ngdy thdng qua phdn bidn: 29/5/2015 Ngdy duydt ddng: 5/6/2015
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 9/2015
