S p d i i sa 17. thang 4-2016, trogn, Oai hgc Tay Nguyen ISSN 1859-4611 . ^ ^ ' ^ . ' " ^ ^ ^ ^ ^ " ' ' ^ ™ ^ ^^^^ MAN CAM v o l KHANG SINH CUA VI
KHUAN CAMPrWBACTERSP. TREN CAC LOAI THIT (GA, HEO B 6 ^ T A I TPBUON MA THUOT - DAK L A K
Trin Thj Thanh Van' Cao Van Hfing^
Doan Thj Kim Phirffng^
Ngay nhan bai: 29/12/2015; Ngay phtin bienthgng qua: 21/3/2016; Ngay duyet 4!ng°'2'o/4/Mir''' TOM TAT
mmmssm Ttrkhda: Phdn lap, Campylabacler sp.. Ihiidongvat. khdng sinh. mdn cdm LD4,TVANDE . .,.
V • = „i '"•""E "El"™ "ini nay, chting tgi tiln hanh dugc dat len l^ang dau. Dac biet, ngugi ta rat quan cam eua Campylabacler sp. duoc phan lap tren ttm den cac loa, thue pham ti, dgng vat va san thit ga, thit heg va thit bg vtri mot sii Igaikhang pnam dgng vat b| g nhiem co kha nang gay dgc sinh thgng dung hien nay • • B e h g „ g u g i s u d « . 2. NO. Dt^'G VA PHlTONG PHAP NGHIEN
Campylobacter la mgt trong nhirng tac nhan CUtl
h a ^ dau gay benh tiet; chay o ngugi. 6 nhtmg 2.L Ndi dung nghien etiu nuoc dang phat trten. dgi tugng chii yiu bi nhilm n • >. • . -
Campylobacter tir cac loai san phim dona vat " " ^"' " ° '' °'''™ Campylobacter la tre em dutM 5 R,ai. Ben canh tinh hinh nhilm ''' " " "=" ' " ' ' * ' ' '^^ ''=<'• ''°)-
Campylobacter ngay cang gia lang o ca cac nutje " T'"'' ""^ ' ^ c™ Campylobacter sp, vol phat trien va dang phat g-iln thi ty le cac chiing " " ' ^° ' ° ' ' ''''™8 sinh thutmg diing.
khang thuoc cung khong ngimg tang len [3] 2-^- Phirang phap nghien cdu 0 Viet Nam, Campylabacler dung la mot trong ^-^-'- Phtrangphdp ldy mda
nhttng VI khuan chinh gay tieu chay, dac biet la 6 - Dia dilm lay m5n: eac mSu thit duoc thu tre em ducn 5 moi. Vi khuSn nay chilm 17,63% thap 6 cac cho thuoc cac phuTg x Ea Tam
ren tottg so 363 tnrong hgp do tae nhiin vi khuSn Khanh Xuan, Cu Ebur Thanh S trong 1,420 benh nhi tieu chay tir 06/2009 - r-i,,, ,- ' ^. . -
05/2010 (Don vi Nghien euu Lam sang Dai hoc ,hit'b6 duo T " " ,"•!'• Man ,hit ga. thit heo va Oxfond-OUCRU) ' " " ! ' ' " ' I ' " " : lav o cat: chg tir liie sang stJm. Tren M ' ._• . .= ^l^'^y ^"'^ Chung tgi tien hanh lay ngdu nhien m/tf Nguoi b, nhiem Campylobacter ehii yiu la lugng thit khgttng 50g/miu cho vao mi ni ong Wi do sir dung cac san pham la thit trong do ehu yiu trimg. Miu thit dugc bao quan trong 1 g d ' vl
hu! h' w- "" •" " " " f"; ^"^ « " "'y- " ^ ^ ^ phong xtit nghiem s L n h i t , khangqur24h chua thay nghien cm, nao g Dak Lak no, den ty lc (Theg TCVN 7715-1.2007 - ISO 0272 I 20061 nhiem C<i,n;,r/oit,«cr tren thit ga, thit bo vti thit 2 2 2 Ph,^ u- ' = " ' " ^ " '-^OM)-
• • -^ ^-^ Phuang phap phan Idp vd xdc dtnh I SSTV. Khoa Chan nuo, Thuy. ,ruang Da, hoc Ta,- Ngui-en
1 ^ r , f r ^ r " '"'• 'ru'" '•'"•'™«"" '<" '•* *^™
S BSTY. Khoa Chan nuoi Thuy tnrang Bai hoc Ta,- Nguyen 4 Ky Su. Khoa Chan audi Thuy truang Dgi hoc Toy- Nguven
Tdeg,ahenhe- Tran Tht Thanh Van : DT 094439,074. Ea,ad ,r.,ha„h,-,ndh,a%yahao con.
13
Tapchi sd 17. ihdng 4-2016. Irudng Dai hoc Tdy Nguyen ISSN 1859-4611
Campylobacter sp.
Su dung phuong phdp thudng quy ky Ihuat phdn ldp va xdc dinh Campylobacter trong thuc phdm". 52 TCN-TQTP 0014-2006. Bd > t^ (2006).
2 2 3 Phirang phdp ihu nghiem khdng smh do Bang phucmg phdp Kirby-bauer.
2 2.5. Plurong phdp xir ly sd lieu
Sd heu diroc tdng hop va xu ly tren phan mem
Ket qud tren bang 1 cho didy ty I? nhi&n Campylobacter tren cac lojti thit dugc khao sat ia 7,26%. Trong do, ty le nhiem Campylobacter tren thit ga cao nhat la 8.96%, ti^p dSn tren thit heo la 5,00%. va tren thit bd la 5.56%. Theo nghien cihi cua Nguyen Due Hiln, Huynh Minh Tri, Le Nghia Trong (2014) cho thay ty Ie nhilm Campylobacter tren thit ga d mot sd chg tai C i n Tho la 9,38%, ket qua nghien ciiu ciia chung tdi phu hgp vdi nghien Excel va phdn mem thdng ke sinh hoc Minitab cuu nay. So vdi mdt so khdo sal da thyc hi|n.
_!.:=_ i-^_ t£ chiing tdi nhan thdy ty le nhilm Campylobacter tren thjt ga tai thanh phd Budn Ma Thuot thap hon nhu tai Bac Ninh la 49,38% (Lun Huong Quynh va cs, 2009), hay Ha Noi la 3 1 % (Luu HuoTig Quynh va Trdn Thi Hanh, 2006). Tuy nhien sir khac nhau nay la do dieu kien thdi ti^t khi hdu tren timg dia phucmg khac nhau.
Theo nghien ciiu ciia Orla A. Lynch, Claire Cagney, David A. McDowell, Geraldine Duffy (2011), ty le nhilm Campylobacter a thjt bo la 36%, d thit heo la 22% va d thit ga 16%, ket qua nghien ciiu nay cao hon so vdi nghien ciiu ciia chiing tdi. Do dilu kien khi hau, che do cham soc khac nhau nen ty 1^ nhiem Campylobacter nghien ciJTj d cdc nudc khde nhau thi cd su sai khde nhau.
3.2. Ket qud nghien ciru tinh mdn cdm cda Campylobacter sp. v&i mgl so logi khdng sink thong dung hien nay
Ket qua thtr nghiem khd nang mdn cdm ciia 18 chung Campylobacter sp. vdi mgt sd loai khdng sinh thong dung, dugc trinh bdy d bang 2.
phien ban 16.
3. K E T QUA T H A O LUAN
3.1. Tinh hinh nhiem Campylobacter sp. trin cdc doi lugng thit (gd, heo, bo)
De khdo sdt tinh hinh nhiem Campylobacter tren sdn pham thit ddng vdt tai thdnh phd Budn Ma Thugt, chimg tdi tien hdnh thu thdp cdc loai mdu thit khac nhau d mgt sd cho, kit qud Ihu dugc trinh bdy d bdng 1.
Bang 1. Ty le nhicm Campylobacter sp. tren cac doi tirtmg thit (ga, heo, bd) Doi tugng thit
Ga Heo Bo Tong
SMNC 134 60 54 248
SMN 12 3 3 18
T y l e ( % ) 8,96 5,00 5,56 7,26 SMNC Sd mdu nghien ctru. SMN. Sd mdu nhiem
Bang 2. Ket qua thir nghiem linh min cam cua Campylobacter s p . vdi mot sd Ioai khang sinh
^^^y (S) Trung gian (I) K h a n g (R) Ten khang
sinh
So chung
thu- So m a u Ty le (%) So m a u Tyl£ (%) So mau Ty lc (%)
J 4 5
8
10 Ampicilhn Amgxicillin Trimethoprim Nalidixic acid Azithromycin Otlo.xacin Cipro lloxacin
Erythronncin Gcntamvcin
18 18 18 18 18 18 18
IS 18
10 3 1 3 16
17 18
55,55 16,67 5,56 16,67 88,89 16,67 16,67
94.44 IOO
1 0 0 0 0 0 1 0 0 0
5,56 0,00 0,00 0,00 0,00 0,00 5,56 0,00 0,00 0,00
7 15 17 15 2 15 14 15
0 38,89 83,33 94,44 83,33 11,11 83,33 77,77 83,33 5,56 0,00 i.> le man cam cua CampvhhaCcr v
Gentamvcin cao nhit (100%). tijp ,i,eo Etythromvcin vcri ty le la 94.44%. Azithromyc
la 88,89% Doi vol Ampieiilin thi CampylobaCer eo ty' le min cam trung binh la 55,55%. Con lai. Amoxicillin, Nalidixic acid. Ofloxacin,
P chi s6 17, thiing 4-2016, tnrcmg Dai hgc Tav Nguven
ISSN 1859-4611 Ciprgflo.xacin. Ceftriazone voi t) le la 16,67%.
ty le Campylabacler man cam thap nhit l i voi Tnmethoprim - Sulfametho.xazole (5.56%). Vav Campylohacer di khang vgi rit nhilu loai khan'g smh thong dung hien nay, cho nen viec lua chon khang sinh p h i hgp d l dilu tri benh do vi khuin Campylohacer gay ra la rit cin thilt. Theo mgl so nghicn cih, ttuoc day ehg thiv. Theo En»ber"
J.. F. M. Aarestrap, D. E. Tav lor. P Gemer-Sraidt and 1, Nachamkin (2001) tj Dire, tv le eac c h u n . Campylobacter phin lap tir nguiri khang v6i Ciprgflg.xacin la 41 - 46%. trong khi co ttji 42% v a 71 % cac ehiing C. jejuni va C. coli phin lap tir thit ga la khiing v oi Ciproflo.xacin.
Hien nay Macrolide dugc .xem nhu la khang smh tgi uu dc chiia trj benh dg Campylobacter Mac dil Erxlhrgmycin da dugc sit dung tir rit lau nhung khuynh huijng khang khang sinh niv o Campylobacter thiip. V i o nam 2010, tnrcmg Dai hgc Thuy Dira da ehi ra ring ti le Campylobacter phan lap tir thit g i <j Can Thg khang vtii Nalidixic acic va Ciprgflo.xacin da tang len din 64% d l i vdi
C. Jejuin vi len din 100"^ doi vtji C colt.
So vtji nhirng nghien cuu tnrtjc dav. nghien cuu ciia chiing toi eho thiy ring ty l e v i khuin Campylabacler khing Ciprofloxacin ngay cang tang len. Theo chting toi. vin d l su dung"khang smh trong thire an chan nugi eung l i mot nguven nhan dan den tinh trang khang khans sinh ngav cang tang len [3].
•t, K E T LUA-N
- Ty" le nhiem Campylabacler tren eac loai thjt dugc khao sat la 7,26%. Trong do, tx le nhilm Campylohacer tren thjt ga la 8.96%, tj le nhilm o thit heo la 5,00%, va tren thit bo la 5,56%.
- Tv le man cam ciia CampylobaCer vtji khang sinh Gentamvcin cao nhit (100%), tilp theg li Erxlhrgmycin vtji t> le la 94,44%. Azilhrgmvcin la 88.89% C i c khang sinh nhu Amgxicillin Nalidixic acid. Ofloxacin. Ciprofloxacin,' Ceftriazone ctj xy le min c i m thip (16,67%), dac biet tx le Campylobacler m i n c i m thip nhit viji Tnmethoprim - Sulfamethoxazole la 5.56%.
Tran Thi T h a n h Van' Cao Van Hong^
Doan Thi Kim Phuong^
Received Date. 2 9 / 1 2 ^ 0 , 5 : Revised Date: 21-3.2016, Accepted tor P u b l i c a t i o n ' ! ^ : 2 l r ' " "
S L M M A R Y
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Keywords lsola,e CampylobaCer sp meat .Knlnin- aniihialic
''':/'""''"''"""'•'""•"'•"""•n Science To, \g,„„,L - I liD . Depar,men, o/ln.,pec„on Ta, \gu\en Ln„ers,r,' I Ve, Fac„l„ ;!in,malanJ,e,er,nan Scence. Ta, \g,i,en C ICng lucdn of.-,n,mal and Ve,erinan.Science. To, \ . , , „ „ , [ :orr,-.,p„„J,ng au,hor Tran Th, Tl,anh 1 ' " '
Cellphone 0944,910-J Cmail ,r,l,u„lna 15
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Tap chi 56 ! 7. thdng 4-2016. trudng Pat hoc Tay Ngu\'en ISSN 1859-4611
TAI LIEU THAM KHAO Tai lieu tieng Viet
Bd Y t^ (2006). "Thudng quy k> thuat phdn lap va .\.ac djnh Campylobacter trong thuc phdm", 52 TCN- TQTP 0014:2006
Luu Quynh Huong. Trdn Thi Hanh (2006), Dieu tra ve t>' le nhiem Campylobacter spp. Tir thit ga thu thap tren dia bdn Ha Npi. Tgp chi khoa hoc thdy-Jap Xlll - sd 5 -2006, trang 49- 53.
Luu Quynh Huong, Tran Thi Hanh, Pham Thj Ngoc, Truong Thi Huong Giang (2009), Phan lap, dinh t>p va xac djnh khd nang khdng khang sinh cua vi khudn Campylobacter spp. d nhiem tren thit ga tai Bic Ninh, Tgp chi khoa hgc thiiy - Tdp XIII - sd 3 - 2009, trang 40 -44.
Nguyen Diic Hien. Huynh Minh Tri, Le Nghia Trong, Tinh hinh nhilm Campylobacter tren gia cam kinh doanh tai thanh phd Cdn Tho. Tap chi khoa hoc thu y -Tap XX] - s 6 3 - 2014, trang 29-36.
TCVN 7715-1:2007, Tieu chuan qudc gia - Ha noi. Vi sinh vat trong thuc pham vd thiic an chan nuoi - Phircmg phdp phdt hien va dinh luong Campylobacter spp., 2007, p 9.
Tai lieu tieng Anh
Bulzler, J.P, and J. Oslerom (1991), "Campylobacter pathogenicity ang significance in food", Int J Food Microbiol, 12, p. 1-8.
tngberg, J., F. M Aarestrup. D. E. Taylor, P. Gemer-Smidt, and I. Nachamkin (2001), "Quinolone and macrohde resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates". Emerg hifect Dis. 7, pp. 24-34.
OriaA Lynch, Claire Cagney, David A McDowell. Geraldine Duffy International ... C M Burgess G Duffy, Jan2011:pp.l44-168.
