VIETNAM MEDICAL JOURHAL ^"2 - JANUARY - 2017
LIEN QUAN DAC DIEM HINH THAI NOAN vol KET QUA THU TINH SAU ICSI
Nguyin Thi Linh\ Nguyin Xuan Hffi^, Nguyin Manh HS^
T O M T A T
Nghien cijfu nham danh gia mdi lien quan mot so dac diem hinh thai riogn tfli ket qua thy tinh cua_benh nlian thu tinh trong ong nghiem (TTTON). 414 noan thu du'dc cua benh nh§n kfch thich buong tru'ng bSng phac dQ Antagonist tai khoa Ho trd sinh san b§nh vien Phu san Ha Noi. Ket qua thu tinh anh hUflng bfli hinh thai khoang quanh noan (p=0.017). Ty- l§ thoai hoa ncran sau ICSI li§n quan den mau sSc mang trong suot ,(p=0.01, OR=3.4, 95%a=1.6-7.0), dp rpng khoang quanh noan (p=0.01), su' xuat hien h^t d khoang quanh noan, (p=0.01, OR.=3.6, 95%a=1.5-8.7), 65 min cua bao tu'dng (p<0.01), xuat hien quang hat tho trong no3n (p=0.005, OR=2.2, 95%a=1.3- 3.7), do dan hoi cua mang bao tu'dng (p<0.01).
Tdkhoa: Hinh thai noan, ICSI, thu tinh, thoai hoa noan
S U M M A R Y
A CORRELATIVE STUDY OF OOCYTES MORPHOLOGY W I T H FERTILIZATION AFTER
INTRACYTOPLASMIC I N J E C n O N ( I C S I ) This aim of this study was to assess the correlation between oocyte morphology and fertilization rate after intracytoplasmic injection (ICSI).
This descriptive study carried out in 414 oocytes from women undergoing ovarian stimulation by antagonist protocol in Assissted Reproductive Department of Ha Npi Obstetric and Gynecology Hospilal. The fectors affecting tiie success rate of ICSI included perivitelline space (PVS) morphology (p=0.017). The oocyte degeneration rate after ICSI was atfected by colour of zona pellucida (ZP) (p=0.01, OR=3.4, 95%a=1.6- 7.0); enlarge PVS (p=0.01); coarae granulation In PVS (p=0.01, OR=3.6, 9 5 % a = 1.5-8.7); granular cytoplasm (p<0.01); central located granular in cytoplasm (p=0.005, OR=2.2, 95%a=1.3- 3.7); the plasticity of oocyte membrane (p<0.01).
Keywords: oocyte morphology, ICSI, fertilization rate, oocyte degeneration
I.DATVANDE
Noan cd vai tro quan tnang trong sd hinh thanh va phat trien cua phoi. Sir hinh th^nh va
^Kiioa Ho trd sinh san, Binh Win Phu san Ha Noi,
^Trung tam Ho trd sinh san Quoc gia,
^Tn^ng Dai ha: Y Ha Noi, Chiu trach nhiem chinh: Nguyen Thj Unh Email: [email protected] Ngay nhan bai: 24.10.2016 Ngay phan bien khoa hge: 20.12.2016 Ngay duyet bai: 28.12.2016
phat trien cOa noan chiu anh hu'dng bdi cac*^
td vi mdi tru'dng do buong tru'ng va nang trifng tiet ra. Trong chu ky tu" nhien, phan Idn cac nanf tru'ng thoai trien, ehi ed mot nang triJng du"^' chon Ipc phat trien vu'dt troi va gay phong noan hang thang. Chinh vi tlie, viec sd dung cae t h i ^ ndi tiet de kfeh thich buong tru'ng, gay tru^c^' thanh nhieu nang tru'ng hdn thay vi bi thoai trien cd the anh hu'dng tdi chat lu'dng noan, Ch^
lu'dng noan du'dc danh gia tren nhieu khia cantll hinh thai, cau true di truyen, cau true phan t ^ sd chuyen hda. Tai cac trung tam TTTON vile danh gia chat lu'dng noan chu yeu dda tren hirih thai dddc quan sat tren kinh hien vi quang h ^ khong can thiep khdng anh hu'dng tdi noan,
Trong ky thuat IVF co dien, viec danh gi4 hinh thai noan gap nhieu kho khan do co sir bao phu cua te bao hat quanh noan. Nam 1992, vdi su" ra ddi eua ky thuat tiem tinh tri!ing vao bao tu'dng noan (Intra cytoplasmic sperm injection- ICSI), nhd tach bd te bao hat ma nhieu yeu to hinh thai noan dddc d^nh gia ehi tiet hdn:1!|' tru'dng thanh eua nhan, bao quan, hinh. thii mang trong sudt, th^ cdc thuf nhat, thoi vo sac.
Cung trong nam nay, IBn dau tien Van Blerkom va Henry de cap tdi khai niem "cytoplasmic dysmorphisms"- nhiJng md ta bat thu'dng ve bao tdcJng hoac mot sd khiem khuyet eua hinh thai noan [1]. Tren the gidi da ed nhieu nghiin cuti ve mdi lien quan gliJa hinh thai noan va ket qia thu tinh sau^*ICSI; tuy nhien, ket qua cua die nghien cdu nay van cdn chu^a thdng nhat. Vi va^
chung tdi tien hanh nghien euli danh gia^^
nhiiTig yeu td hinh thai noan anh hudng denll thu tinh cua ky thuat ICSI va mut do anh hiTclflf eua nhGtig yeu td nay.
II. DOI TU'QNG VA PHirONG PHAP NGHIEN CUU 1.1. Doi tu'dng nghien cu'u
Toan bo noan I^II dddc thu tinh vdi tJnh triling bang phu'dng phap ICSI ti^ thang 12 nam 2015 den thang 7 nam 2016 cua cac cap vrf chong thda man dieu kien sau:
• Vd ki'ch thich buong trdng bang phac do Antagonist
• Chong cd tinh djch do binh thu'dng tlieo tieu chuan cua WHO 2010,
1.2. Phu'dng phap nghien cll^u
126
TjJ> CHl Y HOC VIET NAM TAP 450 - THANG 1 • SO 2 - 2017 Nghien cdu md ta tien euli thdc hien tren
1 noan du dieu kien tren. Danh gia chat Ing noan, thu tinh, phdi theo tieu chuan dong ian ciia Alpha/ESHRE va ehi hdi Y hpe sinh san
*Nam.
• TiemtmhtnffigiaobaotidJhgnoan(ICSX}
Benh nhan du'dc choc hut noan vao thdi
•m 36 -40 gid sau khi tiem hCG, Noan sau khi 1 nhan se dddc nudi tiep trang mdi tru'dng G- F (vitrolife). Sau 2 gid tien hanh tach noan va oi tiep tue noan sau tach trong mdi tru'dng G- F. Sau 2 gid tien hanh ICSI. Moi noan dddc :a vao 1 gipt Spl mdi tru'dng G-Gamete Itrolife) cd phu dau. Danh gia chat lu'dng noan:
lh dang, mau sac mang trong sudt, hinh thai oang quanh noan, the cdc thd nhat, tinh chat 0 tddng, dp dan hoi mang bao tddng.
Giu* noan bang 1 vi kim d vj tri 9 gid so vdi B arc. Dung 1 vi kim khae bat gid tinh triling va im thang vao bao tu'dng noan d vj tri 3 gid so liotlt^dc- Moi noan sau khi ICSI ddpc nudi cay
^yiggipt mdi tru'dng Gl (Vitrolife) ed phu dau eodung thdtu'vLfa ICSI.
• 0anh gia tiiu tinh:
Tliu Bnh dddc danh gia 16- 18 gid sau khi SI. Noan dddc danli gia la thu tinh khi xuat en hai tien nhan va 2 the CLTC. Trddng hdp
noan cd ba tien nhan va noan thoai hda thi loai bd. Chuyen noan sang dTa nudi mcfl, la dTa Falcon BD 0 60 chu^ gipt mdi trddng Gl (Vitrolife-Thuy Dien) ed phu dau Ovoil, moi gipt mdi trddng ed the ti'ch lOpL, danh so thd td, hdp td van 6d6c gid nguyen thd td nhu' d dTa sau ICSI, nuoi tiep tue trang tiJ eay C02 6% va nhiet do 37*'C.
1.3. Dao dd'c nghien cihi:
Day la nghien aid md ta khong can thiep.
Noan dudc nghien edu thu dutfc td eae cap vd chong lam TTTON da ed cam k§t giij^ b$nh nhan va od quan chu quan la khoa Ho trd sinh san benh vien Phu san Ha Npi. Cac benh nhan ed noan dong y tham gia nghien edu. Cae thdng tin ca nhan do benh nhan cung cap dddc dam bao bi mat III. KFT QUA
Ty le thu tinh gida cae nhom hinh thai mang trong suot theo mau sac mang khac biet khdng cd y nghTa thong ke theo bang 1. Tuy nhien thoai hda sau ICSI g\da eac nhom hinh thai mang khac biet cd y nghTa thdng ke. Nhom mang trong suot sam mau cd ty le thoai hoa eao nhat (35.1%). Mang trong sudt sam mau lam lang nguy cd thoai hoa noan sau ICSI gap 3.4 lan.
Bang 1: Lien quan hinh thai mang trong suot va icet qua thu tinh Dac diem
Sam mau Binh thu'cinq
P OR (95% a )
Thu tinh n 23 266
62.2
%
70.6 0.289
Thoai hoa n 13 52
»/o 35.1 13.8 0.01 3.4 (1.6- 7.0) Bang 2 cho thay lien quan giij^ hinh thai khoang quanh noan va ket qua thy tinh. Cd sd khac biet ii^ ket qua thu tinh eiia eac nhdm hinh thai khoang quanh noan theo phan loai su* xuat hien hat ong khoang quanh noan. Khdng thay sd khae biet ve ty le thu tinh gida eac nhdm phan loai theo dp aig khoang quanh noan. Ty le thoai hoa noan sau ICSI khac biet cd y nghTa g\da tat ca cac nhom ph ttiai mang quanh noan theo dp rpng, theo sir xuat hien hat trong khoang quanh noan. Xuat hien Pttrong khoang quanh noan Iam l^ng nguy cd thoai hoa noan gap 3.6 lan.
Bang 2: Lien quan hinh thai khoang quanh noan va ket qua thu tinh Dac diem hinh thai
Xuat hien hat trong ithoang quanh noan Tlieo iJo rang Idioang
quanh noan (trong nhom khong hat)
Co hat Khonq hat
P OR (95% a )
Binh thu'dng Rat hep Rat ronq
P
Thu tinh n 9 280
»/o 40.9 71.4 0.02 219
27 34
74.2 60.0 65.4 0.084
Thoai hoa n 9 56
40.9
%
14.3 0.01 3.6 (1.5-8.71 31 12 13
10.5 26.7 25.0 0.01 Ti'nh rieng trong nhdm khong eo hat trong khoang quanh, nhdm khoang quanh noan rat hep tang guy cd Uioai hda noan sau ICSI gap 3.1 lan va nhom khoang quanh noan rat rpng tang nguy cd ioai hda gap 2.8 lan so vdi nhom binh thu'dng(bang 3).
VIETNAM MEDICAL JOURNAL W'2- JANUARY - 2017
Bana 3; Yeu to nguy cd do rong Ithoangquanh noan den thoai hoa sau ICSI -?— • r—' ^r, r « M / »-.T\ ^~r mie- _K I n o fQ^' PVS hep
Rat hep Bmh thtfclnq
OR (95% g ) 3.1 (1.5- 6.6)
PVS rong Rat rpng Binh thu'dng
OR (95% g ) 2.8 (1.4- 5.9) Bang 4 cho thay khong co si/ khac biet ve ty 16 thu tinh cung nhU thoai hoa giu'a cac nhom hfih thai the eye thiJ nhat _ , „ •' „
Bac diem hinh thai Kich thuflc binh thuflnq, bS mat trdn Kfch thuflc binh thu'dnq, be mat nhan
Phan manh Tren 2 the cUc, the cUc thoai hda
P
Thu tinh n 1 % 94 1 68.6 130
62 3
74.3 65.3 42.9 0,165
Thoai hda n IS 27 17 3
13.1
%
15.4 17,9 42.9 0.177 Ty le thu tinh cua cae nhdm hinh thai mang bao tu'dng khdng ed sii khac biet. Ty te thu tinh cua nhom m'ang'bao tu'dng kem dan hdi rat thap so vdi nhdm mang bao tddng binh thu'dng. Trong khi.flo ty le thoai hda khac biet gida tat ea eac nhom hinh thai mang bao tddng cung nhu" dp dan hoi mang bao tddng. Sy xuat hi|n quang hat thd trung tam (tddng dng vdi gran dp 3,4) tang nguy cd thoai hoa gap 2.2 IBn. Nhdm bao tddng kem dan hoi tang nguy ed thoai hda gap 95.5 lan.
Bang 5: Lien quan hinh thai bao tu'tfng vdi ket qua thu tinh Dac diem hinh thai
Cd quang hgt tho
Thu tinh
Theo sd xuat hien quang hat thd
trung tam
Khdng quang
0.005
OR (95% g ) 2.2(1.3- 3.7)
Theo dp dan hoi mang b^o tu'dng
Kem dan hoi Binh thddng
14
OR (95% g )
0.00 95.5 (12.3- 742.0) Nhom the vui dp 3 va 4 tang nguy cd thoai hda noan sau ICSI gap 2.7 lan so vdi nhdm nhom tJie vLii dp 1 va 2. Khdng thay sd khac biet ty le thu tinh va thoai hda gida eac nhdm bao quan con lai.
Ludi npi chat trdn it xuat hien trong noan nen ed mau nhd, khdng Iam dddc cac thuat toan thdng ke, chi ghi nhan dddc ti le thu tinh va thoai hda (bang 6)
Bang 6; Liin quan giu'a cac b^o quan vdi k£t qua thu tinh Dac diem hinh thai
The vui
Khong bao LUfll npi chat trdn
Inc dd 3, 4 Inc do 1, 2
P OR (95% g ) Cd xuat hien Khdnq cd
P Cd xuat hien
Khdnq cd
Thu tinh n 19 270
. °/o 59.4 70.7 0.181 6 283
50 70.4 0.129 3 286
60.0 69.9
Thoai hda n 10 55
»/o 31.2 14.4 0.012 2.7(1.2- 6.0) 3 62
0.31 1 64
25.0 15.4 9
20.0 15.6 IV. BAN LUAN
Mang trong suot la san pham che tiet ciia ca te bao hat va noan bao trong suot qua trinh phat trien cua nang tru'ng va noan bao. Nhieu nghign diu cung khong tim thay moi lien quan giu'a hinh thai mang trong suot vdi ket qua thu tinh ICSI nhy nghien culj cua Sutter (1996), Babalan (1998), Khalili (2005). Trong nghien cu'u ciia
chiing toi mau sac mang cd anh hu'dng tdi thoai hda noan sau ICSI [3]. NhuTig noan cdmang trong sudt sam mau thu'dng cd sU xuat hien da khong bao trong bao tu'dng, vi the cd the duS ra gia thuyet mang trong suot sam mau cd lien quan den nhiitig khiem khuyet trong bao tu'dng ntan, trong dd cd nhiiTig tdn thUdng ket cau bao tiidng noan khdng the phuc hoi khi tien hanh ICSI,
TAP CHl YHOC VlgT NAM TAP * 5 0 - T H A N G 1 - 3 6 2 - 2 0 1 7 Tac gia Rienzi va cdng sy" (2008) cho rang
khoang quanh noan rong ladi giam ty le thu tinh (0R= 1.44, 95%a: 1.06- 1.97) [3]. t y 1$ thu tinh d nhdm khoang quanh noan rat rpng cua chung tdi kha thap va ty le thoai hda tu'dng ddi cao (25%), dteu nay c6 the do trong qua trinh ICSI khoang khdng rat rpng bao quanh noan khien noan khdng du'dc ed dinh chac chan, cd
^le b| xoay trong qua trinh dam kim dan den tao 1 vet rach rpng fren mang bao tddng noan khong the phuc hoi sau ICSI.
Ty le thu tinh d nhdm noan cd khoang quanh noan rat hep trong nghi§n cdu thap (60%), tudng tu* nhd trong nghien cdu cua LIM va epng sd cdng bd nam 2014 (58% so vdi 78%, p<0.01) [3]. Cd ttie nhiJng noan cd khoang quanh noan rat hep mdi chi trddng ttianh ve nhan va ehu^ dat dddc sd trddng thanh ve te bao chat. Su* khdng dong bo trong tt-ddng thanh noan dan den giam kha nang thu tinh cua noan, dong ttidi ket cau bao tddng noan kem, dan den l ^ & ^ i i h d a sau khi ICSI.
Nghien cdu cua ehung tdi gidng ket qua cua Ten (2007) khdng tim thay sd khac biet dang ke ve ty le thu tinh giiJa hai nhdm the cde binh tiiddng va phan manh (67,4% va 66,6%)[3].
Tuy nhien ed xu hddng ty le thu tinh ^lam dBn d nhom the edc thoai hda va nhieu the cdc. The 0 c thoai hda cd the la bleu hien cua noan qua ttudng thanh [5].
Nghien edu eua ehung tdi tim ra su* xuat hien cua cac hat tho tap trung thanh lam tang nguy t d noan tiioai hda sau ICSI. Theo Kahraman va (^ng sd (2000) ttii noan xuat hien quang hat tho OP the la bieu hien sd chda tru'dng ttianh ve te l3ao chat [6]. Cd th^ mat dp hat tap trung cao d vJjng ttung tam noan can trd qua trinh ICSI, cung nhd anh hddng tdi ket eau khung xddng te bao, td do giam kha nang thu tinh eung nhd tangnguy cd thoai hoa.
6 nghien cdu cua chung tdi chl gap 2 hinh thai mang bao tddng la mang binh thddng va mang kem dan hoi. Chi cd 15/414 noan thu dddc 03 hinh ttiai mang bao tu'dng kem dan hoi, tuy nhien chi duy nhat 1 noan thu tinh, cdn lai thoai hoa tt)an bp (thoai hoa 93.3%). Do ed mau cua chljng toi tiidng ddi nhd va chi 1 noan khdng thoai hda sau ICSI n§n khi lam phep thong ke nguy cd noan thoai hda 6 nhdm mang bao tddng l^m dan hoi tang rat cao (OR=95.5, 95%g:
12.3- 742). Neu lam mpt nghien edu co cd mau Idn hdn, co the se gap nhieu noan ed mang bao tddng kem dan hoi thu tlnh hdn, cd the chi so OR se thay ddi tddng ddi.
Trong nghiSn cdu eua chung toi, dang bao quan thddng thay nhat ia the vui, khong bao va Iddi npi ehat trdn hiem gap hdn. Chung toi nhan thay Idch thddc t h i vui Idn va sd lu'dng the vui nhiiu ed xu hddng lam giam ty le thu tinh va lam tang nguy cd thoai hda noan (OR=2.7, 95% CI:
1.2- 6.0). Nghien cdu cua Xia nam 1997 khang dinh noan cd the viJi lam giam ty le thu tinh (64.5% so vdi 84.9%, p<0.05) [3]. Can 1 nghien cdu sau hdn ve cau true va thanh phan ciia the vCii de khang djnh cd hay khong ed tae dpng eua the vui ISn chat Iddng noan.
f<4ac dij khong cd sd khac biet ve thong ke gida t^ le thu ttnh eung nhd thoai hoa d nhdm cd xuat hien khdng bao va nhdm binh thddng, nhdng ehung toi nhan thay xu hddng giam ty le thu tinh (50% so vdi 70.4%) va tang ty le thoai hoa (25% so vdi 14.5%) d nhdm cd xuat hidn khdng bao. Cd mlu chu^a du ldn de tim dddc mdi lien quan, tuy nhien cae nghien edu trddc dd cua De Sutter tien hanh nam 1996 cho thay t / le thu tinh khi ICSI vdi noan cd khdng bao giam (40%) so vcfi nhdm binh thddng (69.6%) [3]. SLT hien dien cua khpng bao Idn hoac nhieu khong bao nhd cd the lam sal lech khung xddng te bao, sai leeh vj tri thoi vd sac cd the dan den tinh trijng tiem vao vj tri thoi vd sac dan dSn bat thu'dng thu tinh.
So noan xuat hien Iddi npi chat trdn rat it (4/414 noan) nen kiidng lam dddc phep thdng ke, trong 4 noan eo Iddi npl chat trdn cd 3 noan ttiu tinh va 1 noan thoai hda. Cd the sd xuat hien ludi npi ehat trdn khong gay can trd qua trinh ICSI, khi tiem ehi can tranh vj tri ludi npl chat tixJn.
V. KET LUAN
Cac yeu to dac diem hinh thai noan co anh hddng tdi ket qua thu tinh, chu yeu lam tang ty le thoai hoa noan sau ICSI. Cd the 1 so dang hinh thai noan lam thay ddi ket eau te bao, lam yeu mang te bao ma can cd phddng phap thu tinh khac han che ton thddng te bao nhd IVF co dlin hoac laser ICSI. Van can co nhutig nghien edU tren od mau Idn hdn de khang dinh dieu nay.
Ldi cam dn: Tac gia xin tran trpng cam dn cac bac sT, dieu du9ng, ky thuat vien cua khoa Ho tn? sinh san benh vien phu san Ha npi da giup dd trong qua trinh thde hien nghien edu.
TAI u f u THAM KHAO
1 . Van Blerkom J., Henry G. (1992). Oocyte dysmorphisms and aneuploidy in meotically mature human oocyte after ovarian stimulation.
Hum Reprod, 7: p. 379-390
VIETNAW MEDICAL JOURNAL N*^ - JANUARY - 2017 2. Ebner T., MoSER M., Gemot Tews (2006). Is
oocyte morphology prognostic of embryo developmental potential after KSI? Reproductive BioMedidne Online. 12(4): p.507-512.
3. Rienzi L., Vajta G., and Ubaldi F. (2011).
Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum Reprod Update, 17(1): p. 34-45.
4. Hassan-Ali H., Hisham-Saleh K, El-Gezeiry D., Baghdady I . , Ismaeil I . and Mandelbaum ] , (1998). Perivitelline space granularity: a sign of human menopausal gonadotrophin overdose in intracytoplasmic sperm injection. Human Reproduction, 13(12): p.3425-3430
5. l o h n n y S Younis, Orit Radin/ Nitsa Mirsky, Ido IzhakI, Tatyana Majara, Shalom Barmi, Moshe Ben-ami, (2008). Brst polar body and
nucleolar precursor body morphology is related to^
the ovarian resenre of infertile women. ReproducBvet BioMedicine Online, 16( 6): p.851-858. '%
Kahraman S., Yakin tC, Domez E., Samli H., Bahce M., Cengiz G., SERtyel S., Samli M., Imirzalioglu N. ( 2 0 0 0 ) . Relationship between granular cytoplasm of oocytes and pregnancy >, outcome fbltowing intracyb^lasmk: sperm injection.^:;
Human Refvoducdon, 15(11): p.2390-2393.
Palermo Gianpiero D., Mina Alikani, Massimo BertoM, Liliana T.CoIombero, Fred.
Moy, Jacques Cohen and Zev Rosenwaks (1996). Oolemma characteristics in relation to survival and fertilization patterns of oocytes treated by intracytoplasmic sperm injection.
Human Reproducdonll.iS)'- P-172-176.
ANH Hird^NG COA 6-HYDROXYDOPAMIN \M TE BAOTIET
DOPAMIN 6- N A O C H U 6 T TREN MO HINH GAY BENH PARKINSON
Trinh Thi Ngpc Y^n*, Nguyin Manh Ha*, Nguyen Khang Sffn*
T O M T A T
Tiem 6-hydroxydopamin (6-OHDA) vao 2 vl tr;
tren dudng di cCia bo s ^ tn;c t d tiem den den the van d ben nao phai de danh gia anh hudng cCia 6-OHDA len cac te bao tiet dopamin. 3 tuan sau khi tiem, cac chugt dudc danh gia kha nSng bj benh Parkinson bang test xoay va danh gia ton thUdng mo hoc ben nao tiim 6-OHDA bSng cac phUOng phap hoa mo mien didi, cau true vi the va sieu vi. Ket qua: so \\i(}x\q te bao du'dng tinh v6i marker TH d liem den giam ro ret sau tiem 6-OHDA, mat dp cac sdi Wian kinh va mdc dp huynh quang cua cac sdi than Wnh d die van giam rat nhieu. Ket qua nSy chiing to 6-OHDA lam thoai hoa cac t^n cCing t h i n kinh d t^e van va cac te bao tiet dopamin d liem den.
WAftda/e-hydnBcydopamin, Parkinson, dopamin, ncfton.
SUIVIMARY
EFFECT OF 6 - H Y D R O X Y D O P A M I N E ON DOPAMINERGIC NEURONS I N THE RAT
PARKINSON'S DISEASE MODEL The aim of this research is to evaluate effect of 6- Hydroxydopamine (6-OHDA) on dopaminergic neurons. 6-OHDA was injected into the rat medial forebrain bundle unilaterally. 3 weeks after 6-OHDA injection, amphetamine-induced rotation test was used
*B6 mdn Mo - Phoi, Tnrdng Dai hge Y Ha Ndi Chju trach nhiSm chinh: Trjnh Thi Nggc Yen Email: [email protected] Ngay nhan bai: 24.10.2016 Ngay phan bien Wioa hoc: 22.12.2016 Ngay duyet bai: 28.12.2016
to detemiine if rats had acxiuired Parldnson's disease.
Histological methods and immunostaining were used fiDr damage assessment of dopaminergic neurons. A marked deaease In the number of tyrosine hydroxylase-immunoreactive neuronal perikarya in the substantia nigra (SN) ipsilateral to the injection and striatal fibre density were observed. TTiese results showed that 6-OHDA triggers a degeneration of dopaminergic terminals In the striatum and cell bodies in the substantia nigra.
Keywords: 6-hydroxydopamine, Paricinson's disease, dopamine, neuron.
I. O ^ T V A N O E
Parkinson la mpt dang thoai hda than Idnh tien trien, anh hu'dng tdi 1 - 3 % d3n sS trSn 50 tuoi, Benh dddc dac trutig bdi s d thoai hoa chpn Ipc cac ndron tiet dopamin d nao, mdc dp ttioal hda ngay cang tang lam cac tri$u chijtig eua benh nang dan theo thdi gian. Cho tdi tan bay gid, qua trinh thoai hda than kinh trong benh Parkinson bat dau va dien bien ra sao van chu^
dddc hieu biSt day du dae biet la cd ehe phSn id va sinh hpc te bao. Do vay, cac mo hinh gay benh thdc nghiem van ddng vai t r d quan trpng trong nghien cdu nguyen nhan, sinh ly benh va cae phddng phap dieu tri eua benh [ 1 ] .
Nam 1968, Ungerstedt da xay ddng dddc mo hinh gay benh Parkinson thdc nghiem dau tien bang each s d dung 6-hydroxydopamin. Mo hinh nay sau dd dddc eai thien va t r d thanh md hinh gay benh phd bien nhat trong cac nghien cdu ve cd che va eac phddng phap dieu tri benh. So v #
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