KHAO S A T T Y LE N H I E M ESCHERICHIA COU, SAIIUONELLA «A TON Dir MOT SO KHANG SINH TREN THjT L O N , THIT GA
TAI n/IOT SO TINH THOOC MIEN T A Y NAM BQ
Le Hong Pliong, Vo Minh Chau, Nguyin Minh Hieu, Nguyen Thi Thi, Nguyin Thi Kim Cue, Bui Thi Diim Hang Trung tdm Kiem tra ve sinh thiiy Trung uangII TOM TAT
Khao sat nay nliim danh gia mile do nhiim E.coli, Salmonella va ton du mot sd Idiang sinh tren thit Ion, thit ga tai cac co so gik m6 (CSGM) va co so l<inh doanh (CSKD) thuoc 4 tinh An Giang, Vinh Loiig, D6ng Thap va C&n Tho. Kit qua xet nghiem cho thiy doi voi thit Ion tai co so giet m6, ty le nhiSm E.coli la 38,89% va Salmonella la 14,44%, tai co so kmh doanh, ty le thit lgn nhiim E.coli la 11,11% va Salmonella la 21,li%. D6i vol thit ga tai co so giit m6, ty le nhiim E.coli la 20% va Salmonella la 15%, tai co so icinh doanh, ty le tMt ga nhiim E.coli la 62,5% va Salmonella la 37,5%. Ket qua idiao sat ton du khang sinh tren 54 mlu thit Ion va 24 mlu thh ga cho thiy Enrofloxacine vugt muc cho phep 6 thit Ion la 2/44 miu (3,7%) va o thit ga la 2/24 miu (8,33%).
Tic khoa- Thit lgn va thit ga, E. coli. Salmonella, ton du khang smli, miin Tay Nam Bo.
Survey on Escherichia coll, Salmonella contamination and residues of some antibiotics in porl< and chiclcen meat in some provinces of Southwest region
Le Hong Phong, Vo Mnh Chau, Nguyen Minh Hieu, Nguyen Thi Thi, Nguyen Thi Kim Cue, Bui Thi Diem Hang
SUMMARY
The objective of this survey aimed at assessing the level of E.coli, Salmonella contamination and residues of some antibiotics in pork, chicken meat at the slaughterhouses and business establishments in 4 provinces of An Giang, Vinh Long, Dong Thap and Can Tho. The surveyed results showed that at the slaughterhouses, the rate of pork contaminating with E.coli was 38.89% and with Salmonella was 14.44%; in the business establishments, the rate of pork contaminating with E.coli v^as 1 1 . 1 1 % and with Salmonella was 27 7 8 % . At the slaughterhouses, the rate of chicken meat contaminating with E.coli was 2 0 % and with Salmonella ^N as 15%, while atthe business establishments, the rate of chicken meat contaminating with E. co//was 62.5%
and with Salmonella was 37.5%. The surveyed results on antibiotic residues from 54 samples of pork and 24 samples of chicken meat showed that enrofloxacine exceeded the permitted level in pork was 2/44 samples (3.7%) and in chicken meat was 2/24 samples (8.33%)
Keywords: Pork and chicken meat, E coli, Salmonella, antibiotic residue. Southwest region I. DAT V A N D E d^ nong bong cua xa hpi va da tro thanh moi lo
- , cho sac khoe cong dong va ngay cang dien bien Tren the gioi, cac vu ngo dSe thuc pham do ^^^^^ ,^p (^^^^^ ^ ^ ^ ^ ^ j ^ ^ ^ 2011). Hon thS VI khuan chiem tot 70%. Tai cac nu6c chau A, ^^^^ (i,'^„ j^g^g bao cua Bo Y ti, nam 2011 tinh VI khuan Salmonella, Listeria monocytogenes va („ng ngg dpc thue phim dang eo xu huong gia S. aureus la nguyen nhan hang diu gay ra cac tang va anh huong khong nho toi stic khoe cong TO ngo doc thuc phim (Ono H.K. va cs, 2008). dtog. Tir nam 2011 den nam 2015, ca nuoc co Tai Viet Nam, ngo dgc diuc phim dang la van 836 vu ngo dgc thuc pham, vai 25.544 nguoi
47
KHOA HOC KY THUAT THU Y TAP XXVI SO 7 - zul9
mac va 155 ngutri chet. Trong cac tmong hgp ngo doc thirc pham do Bo Y te bao cao, c6 79%
s6 vu do vi khuan, 14% do hoa chat, 4% do vuus va i % do ky sinh tning (Bao cao Y te cong cong, 2015).
Theo Tong cue thong ke Viet Nam, tinh den het nam 2016, khu vuc dong bang song Ciiu Long vai dan sd ban 17.660.000 nguai, trong do, tong dan so ciia 4 tinh An Giang, Vinh Long, Dong Thap va Ckn Tho klioang 6.153.000 nguoi, la mot thi truang tieu thu thirc pham tuai song Idn ciia ca nucrc. Do vay, day ciing la cac dia phuong c6 nhieu nguy ca xay ra tinh trang ngo dgc thuc pham cao neu khong dugc kiem soat chat che.
Xuat phat tir thuc tien tren, van de kiem tra, danh gia va giam sat thirc trang nhiem vi khuan va ton du khang sinh tren thuc pham, cu the la thit lgn va thit ga tir ca so giet mo den ca sa kinh doanh phai dugc thuc hien thuang xuyen. Do do, viec khao sat ty Ie nhiem vi khuan va ton du khang smh tren thit lgn va thit ga tai mgt so tinh mien Tay Nam Bg nham co cai nhin tong quan ve thuc trang, ddng thai la co sd dua ra cac giai pbap khac phuc teong thai gian tai.
II. NOI DUNG VA PHlTONG PHAP NGHIEN ClTu
2.1. Noi dung nghien cmi
- Phan tich, xac djnh, danh gia cac chi tieu E.coli va Salmonella tren thit lgn.
- Phan tich xac dinb, danh gia cac chi neu E. call va Salmonella tren thit ga.
- Phan tich xac dinh, danh gia tdn du mgt so khang sinh tren thit Ign, thit ga.
2.2. Vat lieu
- Cac mSu thit Ign va thjt ga dugc thu thap tu CO sa gi^t m6, ca sa kinh doanh ciia 4 tinh An Giang, Vtnb Long, D6ng Thap va Can Tha.
- Dia dilm phan tich xet nghiem: Trung tam Kiem tra ve sinh thii y Trung uong IL - Thai gian thuc hien: tii ngay 1/5/2018 den ngay 30/11/2018.
2.3. Phucmg phap nghien cihi 2.3.1. Phirffng phdp lay mau
- Lay mau tai ca sa giet mo va ca so kinh doanh de xac dinh vi khuan gay 6 nhiem.
- Liy mlu theo QCVN 01-04:2009/BNNPrNT Quy chuan ky thuat quoc gia lay va bao quan mau thit tuai tu cac ca sa giet mo va kinh doanh thit de kiem tra vi sinh vat.
- Giai ban cho phep cua E. coli va Sahnonella theo QCVN 8-3:20I2/BYT.
- Giai ban cho phep khang sinh ton du theo Thong tu s6 10/2016/TT-BNNPTNT va Thong tus6 24/2013/TT-BYT.
- Lay mau tai co sa giet mo va kinh doanb trong khoang 9 h - I Oh sang.
S6 m l u duo-c lay p h a n b o nhiP s a u : Phan ticti vi khuan
Mlu thu thap lai CSGM Thit ga
MSU thu thap tai CSKD
Phan tich ton dip khang sinh Thjt lan
Mau thu thap tai CSGM So CS .,..
khao ^ ° r g An Giang
VTnh Long Dong Thap Can Tha
So CS ^.
khao ^ ° r 9
Tong cong 9
Thitga S6CS , ;
khao ^ ° r 9 S6CS khao Tong
mlu
2.3.2. Phtrcmg phdp phdn tich
Chi tieu phan tich Salmonella E. coli Chloramphenicol Enrofloxacm Sulfadimidin Tylosin Tetracycline
Khang djnh ton du khang sinh
Phu'O'ng phap xet nghiem TCVN 4829:2005 TCVN 7924-2:2008 ELISA ELISA ELISA ELISA
TK AOAC 995.09 HPLG/PDA LC/MS/MS
2.3.3. Phifffng phdp xit- ly sd lieu H I . K E T Q U A
Ket qua dugc tinh toan va xu Iy thong ke 3.1. Danh gia tyle nhigm vlkhuan trgn tliit Ion theo phuang phap thong ke va phan mem 3.1.1. Tai cff sff giet mo qua 2 dot lay mdu Excel. Ket qua dugc trinh bay qua bang I.
Bang 1. Ty le nhiem vi khuan tren thjt Icvn tai ccy s v gi§t mo qua 2 dcrt khao sat
Gib-i han cho phep (QCVN 8-3:2012/BYT) (CFU/g)
5,0x10^-5,0x10^
(/25g) Khong phat hien
(CFU/g) 5,0x10'-5,0x1 "3
{/25g) Khong phat hign So So TJ le So So Ty le So So Ty le So So Ty ie mlu mau khong mau mau khong mau mau khong m l u mau khong kiem khong dat kiem khong dat kilm khong dat kiem khong dat tra dat (%) tra dat (%) tra dat {%) tra dat (%) An Giang
VTnh Long Dong Thap Can Tho' Tong cong Idcrt Tong c6ng 2 dgi So mlu kiem tfa So mau khong flat Ty le khong dat(%)
10 10 10 15 45
3 10 3 5 21
30,00 10 100,00 10 30,00 10 33,33 15 46,67 45
£. coli 90 35 (1.1x10''-4,5x10^)
38.89 0 7 0 0 7
0,00 70,00 0,00 0,00 15,56
10 10 10 15 45
3 3 5 3 14
30,00 30,00 50,00 20,00 31.11
10 10 10 15 45 Salmonella
90 13(du'an9tinh)
14,44 0 3 0 3 6
0,00 30,00 0,00 20,00 13,33
KHOA HOC KY THUAT THU Y TAP XXVI SO 7 - 2019
Qua bang 1 nhan thay, ty Ie nhiem vi khuan d dgt 2 thap ban dot 1 d ca hai chi tieu E. coli va Salmonella. Dcrt 1, E. coli la 46,67% va Salmonella la 15,56%. Dgt 2, E. coli ia 3 L I I % va Salmonella la 13,33%, ty le co giam nhung khong ldn, tinh chung 2 dgt khao sat thi ty le nhiem E.coli la 38,89% va Salmonella ia {A,AA%. Nbu vay, ket qua khao sat ghi nhan.
An Giang va Dong Thap cac mau khdng nhiem Salmonella nhung co ty Ie nhiem E. coli tang cao ni 30%) len 50%; VTnh Long c6 ty le nhiem E.coli va Salmonella giam dang ke, Ian luot tir 100% xuong 30%o va 70% xuong 30% d ca 2 dgt kiem tra; Can Tho co ty le mau nhiem E.
coli giam tir 33,33% xudng 20" o. nhung ngugc lai ty le mlu nhiem Salmonella tang cao, hJr 0%) Ien 20%.
So vdi bao cao cua Lai Thi Lan Huang, Vii Dure Hanh (2017), kgt qua khao sat cua chiing toi ghi nban ty le nhilm E. coli cung tuong nj la 37,31%, nhung thip hon bao cao ciia Duong Van Toan (2010) la 57,50%, trong khi ty le nhilm Salmonella ciing tuang tu, lan Iirgt la 11,94% va 12,5%.
3.1.2. Tgi Cff sff kinh doanh qua 2 dfft Idy mdu Ket qua dugc trinh bay qua bang 2.
Bang 2. Ty le nhiem vl sInti tren thjt lem tai co* s a kinh doanh qua 2 dot khao sat
Dc E. coli
rt1
Salmonella E. coli e o ' t 2
Salmonella GiOT han cho phep (QCVN 8-3:2012/BYT)
(CFU/g) 5,0x10^-5,0x10=
(/25g) (CFU/g) Khong phat hien 5,0x10' - 5,0x10^
(/25g) K h o n g phat hien
An Giang VTnh Long Dong Thap c l n T h u Tong cpng 1 apt Tong cong 2 6g\
8 6 m l u kiem tra So m l u khong flat Ty le khong
<Jat (%) So mau kiem tra
10 10 10 15
45 Sd mau khong
dat
0 10 0 0
10 Ty
khong dat
(%)
0,00 100,00
0,00 0,00
22,22 S 6 mau kiem tra
10 10 10 15
45
£ co/(
10(5,9x10- So mau khong
dat
0 10 0 5
15
^ - I . S K I O ' ' ) Ty
>e khong
flat (Vo) 0,00 100,00
0.00 33,33
33,33 Sd mau kiem tra
10 10 10 15
45
s6
mau khong dat
0 0 0 0
0 Ty
khong dat
(%)
0,00 0,00 0,00 0,00
0,00 So m a u kiem tra
10 10 10 15
4 5
Salmonella
'
25 (du' 90
o n g tinh)
27 78 S o m a u khong
dat
0 0 5 5
10 Ty
le khong
dat
(%)
0,00 0.00 50,00 33,33
22,22
Qua bang 2 nhan thay, ty Ie nhilra E. coli va Salmonella deu giam, Ian lugt tir 22,22%
va 33,33%i xuong con 0%j va 22,22%, mac dii ty le giam cua Salmonella khong dang ke, tinh
Chung ca 2 dm thi ty le ,thi5m trung binh cua E coh va Salmonella la 11.11% va 17 750/ jfl,^
vay. kit qua khao sat ghi nhan. cic mlu'lay tir An Giang dam bao cac chi tieu •,, 5,,,,,. ^^^ ^-^^
tir Vinb Long giam 100%> ty le nhilm E. coh Dang Thi Mai Lan va Dang Xuan Binh (2016) 6 va Salmonella; cac mlu tir D6ng Thap khong 4 tinh phia hk Viet Nam la 11,45%. Trong khi nhiem £ co/; nhrnig CO ty le nhiem ^a/mone/Za A- ^-^ - r- r-., ^ , , . . , , , 7:
1- cno/ ' - .^' r-" T-i. 11-^ ' do,ketqua£. co/ztbaphonbaocaocuaNsuyen tang cao len 50%; cac mau tu Can Tha khong CO ^ -^
thay d6i ty le nhilm E. coh va Salmonella qua ^^^"^ ^ ° ^ ""^ ''^"S "'" ^2016) tai cac ca so kinh 2 dot khao sat doanh a Binh Dinh la 13,35%.
Kit qua ty le nhiem Salmonella cua khao 3-2- Banh gia ty le nhiem vi sinh tren thit ga sat nay ciing phii hap voi khao sat cua Ngo 3 -, 1 -r • ^ .A x . . _, TT^ rnMA\ . • ' 1 • 1, J 1, !• i o n / J.-^.i. iai Cff Sff giet mo quo 2 afft lay mau Thi Hang (2014) tai co so kmn doanh la 28%, i . J nhung lai cao hon rat nhilu so vdi khao sat cua Kit qua dugc trinh bay qua bang 3.
Bang 3. Ty le nhiem vl sinh tren thjt ga tai c a so- giet mo qua 2 dpi khao sat
Tinh
£ . coli
(CFU/g) 5,0x10^-5,0x10^
D c r t i
Salmonella E. coll
Giffi han cho phep (QCVN 8-3:2012/BYT) (/25g) (CFU/g) K h 6 n g phat hien 5,0x10^ - 5,0x10^
Dcrt2 Salmonella
(/25g) Khong phat hien ]y So S6 T* S6 S6 y.* S6 So ]f ie = = le s s ie >. = le , . - • m a u m a u , . ,• mau mau , , _• mau mau , , ."
khong , . i . . . khong , . j . . . khong , .= , ^.. khong
^ kiem khong =• kiem khong " kiem khong - "
(%) " • " ' ' ^ ' (%) " • ' ' ' ^ ' (%) ' " ^ ? ' (%) An Giang
VTnh Long D6ng Thap Can Tho' Tong cong 1 dot Tong cong 2(3ot So mau kiem tra So m l u khSng dat Ty le khong flat (%)
5 5 5 5 20
0 5 0 0 5
0,00 100,00
0,00 0,00 25,00
5 5 5 5 20 E. coli
4C 8 ( 5 , 2 x 1 0 = -
1
-5,7x10^;
20,00 0 3 0 0 3
)
0,00 60,00 0,00 0,00 15,00
5 5 5 5 20,
0 0 1 2 3
0,00 5 0,00 5 20,00 5 40,00 5 15,00 20
Salmonella
40
6 (du'O'ng tinh) 15,00
0 0 1 2 3
0,00 0,00 20,00 40,00 15,00
Qua bang 3 nhan th4y, ty le nhilm bao tot cac chi tieu vi khu4n a dgt 1, trong khi Salmonella d ca 2 dgt khong thay ddi la 15%, ty le nhilm E coh va Salmonella a dgt 2 lai trong khi ty le nhilm E. coli giam it, hi' 25% a tang cao tir 0% Ien l§n lugt la 20% va 40%.
dot I xudng con 15% d dgt lay mlu lln 2. Qua Ngugc lai, mlu \ky tix Vinh Long dam bao 2 dgt khao sat, chi co duy nhat mlu lly hi An tdt cac chi tieu vi smh a dgt 2 nhung co ty le Giang dam bao tdt cac chi tieu vi khuin, cac nhilm E. coh va Salmonella 6 dgt I la kha cao, mau lly tir D6ng Thap va Cln Tha ciing dam lan lugt la 100% va 60% so vai dot 2.
KHOA HOC KY THUAT THU Y TAP XXVI SO 7 - 2019
Ty le nhiem Salmonella tren ga tai ca sa (2016) la 43,3%).
gilt mo ciia khao sat nay thap hem bao cao ciia , , , „ . . . , . , . , •> A ^ iSv mdu XI = ^,- c- • r. -n, u- r- /^m^^ 3.2.2. Tm Cff Sff kinh doanh qua 2 30 lay mau Nguyen Van Sim va Dao Thi Ha Giang (2016)
la 19,23%) va ciia Pham Thi Ngge va cgng su Ket qua dugc trinh bay qua bang 4.
Bang 4. Ty le n h i i m vi khuan tren thit ga tai c a s a kinh doanh qua 2 dcrt khao sat
E. coil
Do4 1 Qai 2 Salmonella E. coli Salmonella Gio-i han cho phep (QCVN 8-3:2012/BYT)
(CFU/g)
Tinh 5,0x10^-5,0x10= {/25g) Khong phat hien
(CFU/g) 5,0x10^-5,0x10=
(/25g) Khong phat hien So So
mau mlu kiem khong
Ty
>e khong
dat
(%)
mau mau kiem khong
Ty 'e khong
dat {%)
mSu mau kiem khong
Ty le khdng
Sd Sd mau mau kiem khong
An Giang VTnh Long Dong Thap Can T h o Tong cpng I d o l Tong cpng 2 d o t So mau kiem tra S d m a u khdng dat Ty le khong d?t {%)
5 5 5 5 20
0 5 5 5 15
0,00 5 100,00 5 100,00 5 100,00 5 75,00 20
£. coli
40 2 5 ( 5 , 4 x 1 0 ^ - 8 , 7 x 1 0 '
62,50 0 5 0 0 5
0,00 100,00
0,00 0,00 25.00
5 5 5 5 20
0 0 5 5 10
0,00 5 0,00 5 100,00 5 100.00 5 50,00 20 Salmonella
4 0 15 (diKTng tfnh)
37.50 0 0 5 5 10
0,00 0,00 100.00 100,00 50.00
Qua bang 4 nhan thay, ty Ie nhilm E. coh c6 giam dang ke, rtr 75% dgt liy mlu lln I xudng 50% a dgt lay mlu lan 2, song ty le nhiera Salmonella lai tang tir 25%o 6 lan lay mau 1 len 50% so vdi lan lay mau 2, tinh chung 2 dgt khao sat thi ty le nhiem E. coh va Salmonella kha cao.
lln lugt la 62,50% va 37,50%. Nhu vay, kit qua khao sat ghi nhan, mlu thit tai An Giang dam bao cac chi tieu vi khuan; raau khao sat tai VTnh Lon2 giam 100% ty le nhiem vi khuan; mlu lly lln 2 tai Dong Thap va Can Tho c6 ty le nhilm E. coli la I00%va iMem Salmonella la 100%.
3.3. Ket qua phao tich t6n du mgt s6 khang sinh tren thit lgn, thit ga
Qua bang 5 nhan thiy chi co duy nhit chi tieu du Iugng enrofloxacin \'ugt miic cho phep d thit
!gn la 3,7% (2/54 mlu). d thit ga la 33,33%, (2/24 mau). Ket qua cua khao sat na> r., han so voi cac bao cao cua Chir Van Tult va cong su (2016) voi ty le nhilm enrofloxacm tren [hit ga la 3,0% Trong khi do, dieo khao sat cua Le \ an Du va cgng sir (2017) tren SO mlu thit Ign vk 70 mau thit ga dTp HCM cho thay ty le t6n du enrofloxacin teen thit Ign ia 0%. nhung dren dut ga len i6i 32 ggo/
Thit Ion
Thit ga
Bang 5. Ty l§ mau ton dif kha
Giai han cho Chioramphenicol (ppb) Khohg phat hien Tetracycline (ppb) 200 (ppb) Enrofloxacin (ppb) 100 (ppb) Sulfadimidin (ppb) 100 (ppb) Chloramphenicol (ppb) Khong phat hien Enrofloxacin (ppb) 100 (ppb) Sulfadimidin (ppb) 100 (ppb) Tylosin (ppb) 100 (ppb)
IV. K £ T LOAN
ng sinh tren thjt liyn
An Giang
Tinh VTnh Long
Dong Thap
,thjt
Can The
ga
Tong Khang djnh duxyng tinh bang LC/
MS/IVIS phep (Thong tu s i 10/2016/TT-BNNPTNT va Thong tu sO 24/2013nT-BYT)
So m i u kiem tra S6 m i u khong dat Ty ig khong dat (%) S6 mau kiem tra S6 mSu khong dat Ty ie khong dat (%) S6 m i u kiem tra S6 mSu khong dat Ty ie khong dat (%) S6 m i u kiem tra 86 miu khong dat Ty le khong dat (%) S6 mau kiem tra 86 miu khong dat Ty ie khong dat (%) 86 m i u kiem tra S6 m i u khong dat Ty i§ khong dat (%) S6 miu kiem tra 86 miu khong dat Ty ie khong dat (%) So mau kilm tra S6 m i u khong dat Ty ie khong dat (%)
12 0 0 12 0 0 12 2 16,67
12 0
"
6 0 0 6 0 0 6 0 0 6 0 0
12 0 0 12 0 0 12 0 0 12 0 0 6 0 0 6 0 0 6 0 0 6 0 0
- Ket qua 12 0 0 12 0 0 12 0 0 12 0 0 6 0 0 6 0 0 6 0 0 6 0 0
18 0 0 18 0 0 18 0 0 18 0 0 6 0 0 6 2 33,33
6 0 0 6 0 0
54 0 0 54 0 0 54 2 3,70
54 0 0 24 0 0 24 2 8,33
24 0 0 24 0 0
diao sat ton dir khang 0 0 0 0 0 0 12 2 3 0 0 0 0 0 7 2 0 0 0 4 0 0
sinh tren 54 - Ty le nhilra E coli tren thit lgn tai cCT sa
giet m6 cua 4 tinh la 38,89% va Salmonella la 14,44%, con tai ca sa kmh doanh, ty ie nhiem Ecoli trang binh la 11,11% va Salmonella la 27,78%,
_ - Ty le nhilm E. coli tren thit ga tai ca sa giet mo cua 4 tinh la 20% va Salmonella la 15%,, con tai CO so kinh doanh, ty le nhilm E. coli trung Mnli la 62,50% va Salmonella la 37,50%.
mau thit 1cm va 24 mau thit ga cho thay du luang enrofloxacin vugt miic cho phep a thit lgn la 3,7% (2/54 miu); o thit ga la 33,33% (2/24 mSu).
TAI LIEU THAM KHAO 1. Le Van Du, H8 Thi Kim Hoa, 2017. Tmh
hinh ton du chat tao nac, khang smh va nhiem Salmonella trong thit heo va ga tieu thu tat thanh pho Ho Chi Minh. Tgp chi KHKT Nong Ldm Nghiep. s5 5 - 2017
KHOA HOC KV THUAT THC Y TAP XXVT SO 7
2. Chu Van Tuat, Tran Thi Mai Thao, Vu Diing Minh, Pham Tbi Trang, Khuc Thi Sang, Tran Thi Ha, Nguyen Truang Linh, Nguyen Thi Kim Chung, Do Van TTnh, Nguyen Thi Thu Hang, 2016. Nghien cuu ton du mgt so khang sinh va beta-Agonist trong thit tuoi (lgn, ga) va nuoc tieu Ign tai 16 mo d mgt so tinh mien bac Viet Nam. Tap chi KHKT thii y. tap XXIV, s6 5 - 20\6.
3. Dang Thi Mai Lan va Dang Xuan Binh, 2016. Xac dinh ty Ie nhiem va dac tinh sinh vat hoa hgc cua mgt sd vi khuan gay ngo dgc thuc pham tren thit lgn tuai ban tai chg tren dia ban cac tinh mien bac Viet Nam. Tgp chi KHKT thiiy tgpXXIll, s6 6 - 2016.
4. Duong Thi Toan, Nguyen Van Luu, Truang Quang, 2010. Khao sat tinh trang 6 nhiem mgt sd chi diem an toan thuc pham trong thit lgn, thit trau, thit bo tai mgt sd ca sd giet mo tren dia ban tinh Bac Giang, Tap chi khoa hoc phdt triin 8 (3), 2010, 466-471.
5. Lai Thi Lan Huong va Vu Diic Hanh, 2017.
Danh gia miic do 6 nhiem vi sinh vat tren thit d mgt so co so gilt mo Ign tai thanh phd Thanh Hoa, tinh Thanh Hoa. Tap chi KHKT thiiy tap XXIV, i6i-20n.
6. Ngo Thi Hang, 2014. Xac dinh raiic do 6 nhilm vi khuan tren tint Ign tai mgt s6 co sd giet mo va kinh doanh tren dia ban Tp.
Ha Tinh. Luan van thac sy kboa hgc nong nghiep, Dai hgc Nong Nghiep Ha Npi.
7. Nguyen Van Suu va Dao Thi Hoai Giang.
2016. Ty le nhilm vi kliuan E.coli_ va Salmonella tren thit gia cSm sau giet mo ^ai huyen Hihi Lung, Lang Son. Tap chi KHKT Ihuy tap XXUlsd 6-20\6.
8. Nguyin Xuan Hda, Le Hiru Diing, Tran Quang Vui, 2016. Muc do 6 nhilm vi khuan trong thit Ign tai mgt s6 ca sd giet mo va kinh doanh thit tren dia ban thanh pho Quy Nhon, tinh Binh Dinh. Tap chi KHKT thu y tdpXXUI.sd7-20}6
9. Ono H.K., Omoe K., Imanishi K., Iwakabe Y., Hu D.L., Kato H., 2008. Identification and characterization of two novel staphylococcal enterotoxins types S and T, Infection Immunity, 16 (II), pp. 4999 - 5005.
lO.Pham Thi Ngge, Truang Thi Qiiy Duong, Truang Thi Huang Giang, Lim Quynh Huang, Tran Thi Nhat, Dang Thi Thanh Son, Luu Van Ba, 2016. Tinh hmh nhilm Salmonella trong chuoi san xuat thit ga tai mgt so huyen ciia thanh phd Ha Ngi 2014 2015. Tap chi KHKT thii y tap XXIII. sd 5 - 2016.
II.Trieu Nguyen Trung, 2010. Tinh hmh ngo dgc thuc pham 2010 va cac hoat dpng ve sinh an toan thuc phim. Tgp chi nong nghiep va phdt tfien nong thon, so ra ngay 15 - 02.
Ngay nhan 16-7-2019 Ngay phan bien 6-8-2019 Ngay dang I-11-2019
