KHOA HOC CONG NGHE
BEIMH V I E M DA DAY V A RUOT DO PARVOViRUS TREIM CHO T A I PHOIMG MACH THLJ Y IMAM THUY,
TilMH DOIMG T H A P
Tran Van TlianhS Tran Ngpc Bich^, NguySn Thi Y^n Mai^
Keovongphet Phuthavong^, Nguyen Phuc Kh^nh^
T6MTAT
TTii nghidm duac tien hanh nham mue tieu xac dinh ti 1& nhi^m benh Canine Parvovirus (CPV) a cho tii 1 d6n 12 thang tuoi hi tieu chay phan co lin mau tai Phong mach Tliu y Nam Thiiy, tinh Dong Thap tir thang 01 den thang 05 nam 2018. Xac dinh ty le b§nh thong qua phuong phap kiem tra nhanh Parvovirus bilng bp kit Parvovirus Rapid test CPV Ag do Cong ty Bionote cua My san xuat. Danh gia hieu qua dilu tri ddi voi nhiing cho benh co ket qua dirong tinh test CPV Ag. Nhimg cho benh dupe lap benh an theo doi nhu ghi nhan than nhiet, tinh trang mat nuoc, tinh trang tieu chay, tinh trang mat mau, trang thai phan... va miic dp tien tri^n cua b6nh. Sau do tien hanh cap thudc theo phac do dieu tri, lieu trinh dieu tn 5-7 ngay. Danh gia hieu qua dieu tri thong qua nhiing cho con song sot va mue do phuc hoi b$nh ciia chiing. Ket qua cho thay 13 trong tong sd 37 cho bfenh co tri^u chiing tiSu chay phan co lin mau duong b'nh voi Canme Parvovirus, chiem li le 35,14%. Cho ttr 1 den 3 thang tuoi co ti ie nhigm b$nh cao hon 6 -12 thang tudi (61,54% so voi 7,69%) va khac biet rat co y nghia thdng ke. Benh khdng phu thuoc ve gidng va gidi tinh. Cho dupe tiem vac xin phdng benh do Canme Parvovirus, ti le benh thap hon so vdi chd khong duoc tiem vac xin (7,69% so vdi 92,31%). Hieu qua dieu tri phuc hdi tneu chiing benh Parvovirus ci cho la 53,85%.
Tii Idioa: Cho, tieu chay, tile nhiSm, CPVAg test, dieu tri. Bong Thap.
1 . BAT VAN BE
Benh viem da day va ruot do Canine Parvovirus type 2 (CPV-2) gay ra b cho la mot benh truyen nhi&m cap tinh (Hoelzer et al., 2008). Benh nghiem trong doi voi cho con ttr sau cai sua den sau thang tuoi. Tuy nhien, cac dot bung phat benh viem da day va ruot gay tu vong o cho truong thanh do CPV-2 cung diroc ghi nhan (Decaro et al, 2008). Benh xay ra a tat ca cac giong cho, a moi liia tuoi va khong phan biet gioi tinh CTr&n Ngoc Bfch va etv., 2016).
Benh diln tien nhanh, lam chu nu6i cho kho phat hi6n som, khi phat hien cho da benh nang kho dieu tri. Cac trieu chung chinh cua benh: sot, non, tieu chay phin long co lan mau tuoi vcn mui tanh hoi kho chiu d ^ den cho mat nhieu mau, nhieu nuac va chat dien giai, roi loan chuyen hoa, truy tim mach, hon me roi ehet (Miranda et al., 2016). Vi$c xac dinh va phan biet nhanh chong dong vat duong tinh CPV-2 b giai doan nhi&m tning c6 the giiip tnen khai kip thai hd tro dieu tri thich hop cho con benh va giam lan
Truong Cao dang Ndng nghiep Nam bp
^ Tnrdng Dai hpc Chv Tho Email; [email protected]
Tnrdng Dai hpc Savanhnakhet, Lao
truyen CPV-2. Tuy nhien theo Chi cue Chan nu6i - Thu y tinh D6ng Thap (12/2017), hang nam ty le kha cao dan cho cua tinh mac benh tieu chay viem ruot wb\ nhung trieu chiing lam sang nghi do Parvovirus.
Nhung hien tai, thong tin ve benh do Parvovirus gay ra a cho tai tinh Dong Thap con han che. Do vay, vifec nghien ciru benh viem ruot do Parvovirus b cho la van de can thi^t giiip cong tac phong va tri benh nay tren dia ban tinh Dong Thap dugc chu dong hon.
Xuat phat tir thuc te tren, nghien cmi "Benh viem da day va ruot do Parvovirus b chd tai Phong mach Thii y Nam Thuy, tinh Dong Thap" voi mue tieu nham xac dinh ty le b6nh viem da day va ruot do Parvovirus b cho va hieu qua dieu tri benh viem da day va ruot do Parvovirusbc\\6 tai tinh Dong Thap.
Z. PHUDNG PHAP NGHBU CUU 2.1. NOi dung nghien oiu
Khao sat tinh hinh nhi^m benh do Parvovirus gay benh b cho tir 1 den 12 thang tuoi.
Danh gia hieu qua ciia viec dieu tn benh do Parvovirus gay ra.
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11/2018
KHOA HOC C d N G N G H l
2.2. V^t lieu nghien ciiu M^u benh an;
Bo kit Canme Parvovirus Ag Test ciia Cdng ty Bionote cua My: 5 bo kit (mdi bo kit gom 10 test).
Test kit gom vung S (vi tri nho giot), viing T (vach ket qua xet nghiem) va vach doi chiing (viing C).
Trong do gom cac thanh phan nhu chat dem miu, chat dem conjugal, mang nitff-cellulose (giay xet nghiem) va chat dem hap thu. Bg kit Canine Parvovirus Ag Test bao gom: thiet bi xet nghiem;
tam bong vo trimg (dung de lay benh pham); chat pha loang (dung dich dem) va ong nho gigt;
Ong tiem 5 ml, 10 ml va kim tiem so 21G, 23 G va kim canh buom sd 21, 23, 25;
Thudc di^u tri: Dich truyen lactate nnger's, dich truyen glucose 5%, gentamycine 4%, B.complex (2%), vitamin K 0,2%o, vitamin C 10%, atropin sulfate 0,2%o.
2.3. PhiKmg phdp nghiSn cmi 2.3.1. Ddi tuong nhien cuu
Doi tuong nghien ciiu la tat ca cac gidng cho, gioi tinh due, cai b mpi lira tuoi, chua qua va da qua tiem vac xin phdng benh CPV-2 den kham va dieu tn tai Phdng mach Thii y Nam Thuy, tinh Dong Thap, voi trieu chiing: sdl, bo an, 6i, tieu chay phan co iSn mau, phan co miii tanh hoi.
2.3.2. Phuongphip nghien cihi
Nghien ciiu mo ta c^t ngang, ti6n cuu va phan tich.
Tat ca cho tieu chay phan cd ISn mau den kham va di^u tri tai Phong mach Thii y Nam Thuy, tinh Dong Thap dugc thu thap thong tin va chan doan benh. Thong tin thu thap gom: hidi, giong, gidd tinh va tinh trang tiem phong (lap benh an).
Chan doan benh do CPV-2 bang test kit Anhgen Rapid CPV cua Cdng ty Bionote - My tiieo cac buoc sau:
Bud'C 1: thu thap mhi, diing tam bong lay phan b true trang ddi vdi nhung chd benh cd tneu chiing di, ti^u chay phan cd lin mau, phan co miii tanh hoi.
Buoc 2: Cho mlu (tam bong lay mhi phan tir true trang) vao dung dich dem khuay deu cho ra phan, cha 10 giay.
Buoc 3: Nhd dung dich miu da pha vao vimg thu (vi tri S cua test), de yen va doc ket qua sau 5-10 phiit.
Budt 4 dpc ket qua.
Duong tinh: co 2 vach hong xuat hien; mot vach hong d' vi tri C (Control) va mot vach hong a vi tri T (Test) tren test kit.
Am tinh: chi co 1 vach hdng xuat hien 6' vi tri C (Control) tren test kit.
Duong tinh gia: chi co 1 vach hdng xuat hien o vi tri T nhung vach mau tai vi tri C khong xuat hi$n tren test kit (Vi tri C mang y nghia Idem chiing su boat dong cua test kit, vi tri T the hien ket qua xet nghiem. Do vay, vach mau tai vi tri C khdng xuat hien thi dij vach mau tai vi tri T cd xuat hien thi eoi nhu test kit do khong hoat ddng).
Tien hanh theo ddi va dieu tri trieu chung ddi vdi nhirng chd cd ket qua duong tinh voi test kit Antigen Rapid CPV. Hang ngay trudc khi cap thudc, tien hanh khao sat va ghi nhan than nhiet, tinh trang tieu chay, tinh trang mat nud'c, mat man, trang thai phan...va tien lugng ti^n tri^n benh. Sau dd tien hanh cap thudc dieu tri, lieu trinh dieu tn 3 den 7 ngay.
Phac do dieu tri:
Tong dich truyen (lit) = % mat nude + khdi lugng CO th^ (dich truyen bao gom: Dung dich Lactate Ringer's va dung dich glucose 5%).
Gentamycine (4%): Tiem dudi da, 4 mg/kg th^
trpng, ngay 2 lin.
Vitamin C (10%): Tiem tinh mach, 5 mg/kg the trgng/ngay.
Vitamin Ki (1%): Tiem Gnh mach 2,5 mg/kg the trpng/6 gid' (trong truong hgp cho tieu chay mit mau).
Bcomplex (2%): tiem blp 2 m g / kg th^ trong, ngay 1 lin.
Atropin sulfat (0,2%o): tiem blp 0,01 mg/1 kg tiie trgng ngay 2 lin.
2.4. Phirong phap phan tich s6 lieu
Sd lieu thd dugc xu ly va tinh toan tren Excel;
sd lieu tong hgp dugc xu ly bing chi binh phuong (x2) cua chuong trinh thdng ke Minitab 16.0.
3. KET QUA NGHBU ClflJ VA THAO LUAN 3.1. Tinh hinh benh tieu hda b chd
Trong tong sd 84 cho b 1 den 12 thang tuoi den kham va dieu tri tai Phdng mach Thii y Nam Thuy,
78 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 • THANG 11/2018
KHOA HOC CONG NGHE
tinh Dong TTiap da ghi nhan 37 ca cd hieu chiing 6i, tieu chay phan mau nghi ngd do nhiem Parvovirus vdi nhimg tneu chiing lam sang di^n hinh va khdng dien hinh.
Nhu vay, chd mic benh di va tieu chay phan man xay ra d" liia tuoi 1-12 thang tuoi la kha cao vol ti le 44,05%. Ti le nay cao hon so voi ket qua cua Nguyln Thi Yen Mai vi etv. (2018) vdi ti le 29,72%. Dieu nay cho thay, tinh trang cho m l c benh ve tieu hoa dac biet la 6i va tieu chay phan mau la dang dugc quan tam va tim ra phuong phap phdng tri tdt hon.
3.2. Tinh hinh nhiem benh do Parvovirus a chd Trong tong sd 37 ca benh cho cd bieu hien tieu chay mau va di thi cd 13 con chd cho k^t qua duong tinh vdi Parvovirus thong qua xet nghiem voi bo kit Canine Parvovirus Ag test.
Ty le chd benh tieu chay cd lin mau nhiem CPV- 2 la 35,14 %. Ket qua nay thap hon ket qua cua Nguyen Van Dung vi etv. (2017) tai thanh pho Hd Chi Mmh la 43,3%; Tran Ngpc Bich va etv. (2016) tai Cin Tha la 44,03%. So dl k^t qua khao sat cua chiing tdi thap hon cac tac gia nghien ciiu truoc dd cd th^ ly giai nhu sau: thirc te tai Dong Thap nhu cau nudi thii cung cua ngudi dan ngay cang cao, chd la mdt trong nhiing loai thii cung tra thanh sd' thich do vay viec cham sdc nuoi duong va phdng benh cho cho duoc tdt hon nen ty le bdnh thap han, Tuy nhien vdi ty Id benh 35,14% la kha cao, cho thay CPV dang lan rdng d Viet Nam.
3.3. Ty le ch6 nhiem benh do Parvovinis theo gi6itinh
B4ng 1. Ty^ le chd nhi^m benh do Parvovirus theo gidi tinh
Giai tinh Cho due Cho cai Tdng
So lirong cho benh 7 6 13
Tyle(%) 53,85 46,15 100 Bang 1 cho thay ty le chd due bi benh do CPV-2 gay ra la 53,85%, cao hon chd cai 46,15%. Su khac biet nay khdng co y nghia thong ke P>0,05. Ket qua nay phii hgp vdi ket qua cua Gurpreet Kaur (2015), ty le chd due bi bdnh do CPV-2 la 57,69%; chd cai la 42,30%. Cho due cd sir min cam vdi virus CPV-2 hon so vdi chd cai. Ket qua nghien ciiu ve dich te va ky
chu chinh cua CPV-2, ca chd due va chd cai min cam ddi vai CPV-2 la nhu nhau (Srinivas etal, 2013). Ket qua nay cung phii hgp vdi nghidn ciiu ciia TrIn Ngpc Bich va etv. (2016) va Nguydn Van Diing vi etv.
(2018), ca hai tac gia cho ring gidi tinh khdng anh huong ty le nhidm benh do Parvovirusb cho.
3.4. Ty le chd nhilm benh do Parvovkus theo nh6m tu6i
Bang 2. Ty le ch6 nhifem b^nh do Parvovirus dieo Ilia tudi
Lua tuoi (thang) 1 den 3
>3 den 6
>6 dfo 12 Tong
So quan sat (con) 8 4 1 13
Ti le (%) 61,54 30,77 7,69 100 Bang 2 cho thay chd cd do tuoi tir 1 den 3 thing cd ty le nhiem benh do PV-2 cao nhat, vdd ty le la 61,54%. Chd o' dd tuoi Ion 3 den 6 thing tiioi ty le la 30,77%. Cho cd do tuoi lan hon 6 den 12 tiiang tudi, ty le nhidm tiiap nhat la 7,69%. Sir khac biet nay co y nghia thdng kd P<0,05. Ket qua thi nghiem cua chiing tdi phii hgp vdi ket qua vai nghien cuu gin day cua Gurpreet Kaur (2015), khi sir dung ky thuat NPCR chan doan benh CPV-2 cho thay ty le nhiem CPV-2 tren chd 0-3 thang tuoi la 60%; chd 3-6 thing tuoi la 30% va chd tren 6 thang tuoi la 10%. Nhu vay, CPV-2 gay benh tren chd d' mpi liia tuoi nhimg nghiem trpng hon d' chd con (Trln Ngpc Bich va etv., 2016). CPV-2 dugc eoi la mdi de dga nhat doi vdi chd con giua thdi gian cai stra va sau thang tuoi, cac dot biing phat bdnh viem mdt va gay tu vong do CPV gay ra trdn cho truong thanh ciing duoc ghi nhan (Decaro e;'a/., 2008).
Chd d' Ilia tuoi 1 den 3 thang co ty le nhiem benh cao hon rat nhieu so vdd chd d' dd tuoi con lai dugc giai thich nhu sau: ve mat lam sang, benh viem ruot do CPV-2 gay ra hiem khi xay ra d- chd tnrdng thanh, khoe manh va d' chd dudd mdt thing tuoi. Vi ca hai giai doan nay, su nhan Idn cua t^ bao bieu md hoc thap dong thdi khang the thu ddng me truyen con cao. Dac biet ddi vdi chd a giai doan tren 2 thing tuoi la min cam nhat vi liic nay khang the do me truyen sang qua sua dau da b i t diu giam dan hoac do chd me khdng cd midn dich trong thai gian nuoi con. Nhiing khang the nay se dugc loai thai het
N 6 N G NGHIEP VA PHATTRIEN NONG THON - KY 2 - THANG 11/2018 79
KHOA HOC CdNG NGHE trong khoang 6 - 1 0 tuan tuoi, liic nay chd con tro
nen de thu cam nhat. Trong khi benh lam sang hlu nhu pho bien d- cho cai siia sdro do su thay ddi khau phan an, su phat trien nhanh ciia te bao bieu md ruot va su thay doi he vi sinh vat duong ruot, la didu kien thuan lgi de CPV-2 tin cdng (McCandhsh, 1998).
3.5. Tf le ch6 nhi^m benh do Parvovirus theo giong
Bing 3. Ty le chd nhilm benh do Parvovirus theo giong
3 . 6 . 1 ^ le chd nhiSm benh do Parvovirus theo ti le tiem ph6ng
Bang 4. H 1§ chd nhiSm b^nh do Parvovirus ttieo ti 1$
tiem phong
Nhom giong Cho gidng noi Cho giong ngoai
Tong
So luong cho benh
7 6 13
Tyte
(%)
53,85 46,15 100 Bang 3 cho thiy ti le mac benh tieu chay mau do CPV-2 b cac gidng chd npi cao hon so vdi cac gidng chd ngoai (53,85% so vdi 46,1596). Tuy nhien su khac biet khdng cd y nghia thdng ke (P>0,05). Ket qua nay phii hgp voi vdi nghidn ciiu trud'c day cho ring CPV- 2 dang lan rong b Viet Nam va gay tidu chay cho chd khdng phan bidt vd gidng (Trln Ngoc Bich va etv,.
2016). Thuc te ghi nhan cac gidng chd ndi cd gil tri thap, de nudi, nen ngudi dan d- khu vuc ngoai 6, cd tap quan mdi hd gia dinh nudi mdt vai con de giu nha, giii vudn...Gidng cho npi thich nghi vdd di^u kien mdi tnrdng sdng mdt each manh me hon giong chd ngoai. Nhung chiing cd ty le nhidm CPV-2 tuong duong vdd nhdm gidng chd ngoai la do chiing chua duoc cham sdc, nudi duong tot, chua duoc tiem phdng day du; ciing vdi phuong thiic nudi tha rdng, nen de tiep xiic vdi mam benh. Nguac lai, nhdm gidng chd ngoai da phan la duac nhtrng gia dinh kha gia b tap trung khu vuc trung tam va xung quanh thanh phd Cao Lanh, nudi voi mue dich kinh doanh, lam canh, lam ban, ndn van de stic khoe ctia chiing rat dirge quan tam (phuang thiic nudi nhdt, dinh duong tdt va tidm phdng dly dii). Them vio dd, cic gidng chd ngoai da duuc nudi d' nude ta trong thdi gian dai, qua nhieu doi, thich nghi hau nhu hoan toan vdi dieu kien sdng d' nude ta, vi th^ ndn sue dd khang ddi vdi CPV-2 giira gidng chd npi va gidng chd ngoai 11 nhu nhau.
Tiem phong Khong tiem phong
Co tiem phong Tong
S6 lugng cho benh 12 01 13
Tyte(%) 9231
7,69 100 Bang 4 cho thay trong sd 13 con chd bi nhidm benh viem mot do Parvovirus cb 12 con chua qua hem phdng chidm ti le rat cao (9231%) va su khac biet nay rit cd y nghia thong ke (P <0,001). Ket qua nay tuong tu vdd cac nghidn ciiu trude dd cua Gurpreet Kaur (2015), dieu niy cung chung minh ring ngoai dieu kidn cham sdc vl che dp dinh duong thi vie xin cung la mot bien phap toi uu d^ bao ve chd khdi nguy ca nhilm benh.
Ti le chd cd tiem ngira nhung vin nhiem benh va chiem ti le kha thap (7,69%). Viec chd tiem ngira vln nhiem benh do nhieu nguyen nhan cd the do cac bien the CPV-2 ciia vie xin chua phii hgp vdi cac bidn the CPV-2 luu hanh a fliuc dia vi CPV-2 cd 3 bien tii^
khing nguyen, trong khi cac vie xin thdng thudng chi cd 1 bien thg (Martella et al, 2005); cung cd the do ca the chd khdng tao dap ling midn dich ddi vdi vie xin, hoac thdi diem tiem vie xin khdng phii hgp, tiem liic khang the thu ddng con cao gay trung hda khang the, hoac do chii nudi khdng tuan thii theo lich phdng vac xin...
3.7. Hieu qu^ d i ^ tri b^nh do Parvovirus tren chd
Bang 5 cho thiy ti le dieu tri khdi benh viem rudt do Parvovirus gay ra voi ti le chua cao 53,85%.
Ket qua nay thap hon ket qua Tran Ngoc Bich va etv., (2016) vdi ti le dieu tri khoi benh la 84,29%. Hieu qua didu tri chua cao, thuc te da ghi nhan l i do chd dem den di^u tri tre, th^ frang suy nhugc, cho nho con. Ngugc lai ndu chd benh dugc phat hien sam, dem dieu tri kip thdd, the trang map map chua suy kiet, nhiing chd Idn con... thi hieu qua dieu tri khdi benh rat cao. Vi khi vi nit xam nhap vio ca the cua chd se gay ra tinh h-ang tieu chay cd lin mau khi^n CO thg mat nude, mat mau va chat didn giai nghidm ti-png. Vi vay, lieu phap dieu tri hd tra tiiiydn dich v6i
80 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11/2018
KHOA HOC C d N G NGHfi
glucose 5% v l lactate ringer's nhIm mue dich bii lai luong nude va chat didn giai da mat theo phan ra ngoii la mdt phuong phap hieu qua guip cho ca the con vat chdng chgi vdi benh de vugt qua giai doan suy kiet. Phac do di^u tri sir dung gentamycin cd hieu qua tdt trong dieu tri cac benh nhiem triing tieu
hda. Day la loai khang sinh co pho khang khuan rpng, hieu qua tren ca vi khuan Gram (-) va vi khuan Gram (+) dac biet la nhdm vi khuan sinh Beta lactamase. Vi the, thudc cd tie dung chdng phu nhidm tot doi vdd benh viem ruot gay tieu chay phin miu do Parvovirus gay ra.
Sd con dieu tri (con)
13
Bang 5. K^t qua di^u tri chd benh do Parvovirus Ket qua dieu tri
Khoi benh Sd lugng
(con) 7
Tyle(%)
53,85
So ngay dieu tri khdi benh 5-7 ngay
Sd lupng (con)
5
Ty le (%)
74,43
8-10 ngiy Sd lupng
(con) 2
Ty le (%)
28,57 4.KETLUAniVAKBUNGHI
Ty le nhilm benh viem rudt do Tirvovirustren chd a D6ng'Ihapchiem35,14%. Benh viem ruot do ParFOKf/T/s xay ra nhieu va nghidm trong nhat a dp tuoi tir 1 den dudd 3 thing tuoi 61,54%, sau dd giam dan qua cic thing tuoi tiep theo v i khdng phu thudc vao nhdm gidng va gidd tinh. Chd hi nhiem Parvovirus do khdng dugc tiem phdng cd ty Id 92,31%, trong khi chd dupe tiem phdng thi ty le la 7,69%. Hieu qua le di^u tri khoi benh li 53,85%.
TAI UEU THAM KHAO
1. Decaro N, Desario C, Eiia G, Martella V, Man V, Lavazza A, Nardi M and Buonavoglia C.
(2008). Evidence for immunization failure in vaccinated adult dogs infected with canine parvovirus type 2c. New Microbiology 31:125-30.
2. Gurpreet Kaur (2015). Detection of Canme Parvovirus ti'om clinical sample using different nucleic acid detection methods and characterization ofVP2gene sequence of Ueld isolates. 99-132.
3. Hoelzer K., Shackelton L. A , Holmes E. C and Parrish C. R. (2008). Within-host genetic diversity of endemic and emerging parvoviruses of dogs and cats. Journal of Virology 82(22): 11096-105.
4. Martella V , Decaro N., Eiia G and Buonavoglia C. (2005). Surveillance activity for canine parvovirus in Italy. Journal Veterinary Medicine B 52:312-15.
5. Mc CandHsh (1998). Canine parvovirus infection, In:NeilT. German, Canine Medicine and Therapeutics, Fourth edition, pp.127-130, BlackwellScience.
6. Miranda, C , C. R Parrish and G. Thompson (2016). Epidemiological evolution of canine parvovunis m the Portuguese domestic dog population. Veterinary microbiology, 183: 37-42.
7. Nguyen Thi Y^n Mai, Tran Ngpc Bich va Tran Van Thanh (2018). Khio sit tinh hinh benh viem nipt do Parvovirus tren chd tai Bdnh xa Thu y, Tnrdng Dai hoc Can Tha. Tap chi Khoa hgc Ky thuat Thii y, 36-41.
8. Nguyen Van Dung, Pham Xuan Thio, Vu Kim Chie'n, Ken Maeda (2018). Dich te hgc phan hi Parvovirus tren chd nudi tai thinh pho Ho Chi Minh.
Chi cue Thii y thinh phd Hd Chi Minh.
9. Saasa, N., Nalubamba, K. S., M'kandawire, E. andSiwila, J. (2016). Seroprevalence of canine Parvovirus in dogs in Lusaka district, Zambia. / . Vet Med Article. ID 9781357, http://dx.doi.org/
10.1155/2016/9781357
10. Tran Ngpc Bich, Tran Thi Thao, Nguyen Thi Ydn Mai va Nguyen Qudc Viet (2016). Khio sattyld benh do Parvovirus tren chd tu 1 ddn 6 thing tudi d thinh phd Cin Tho. Tap chi Khoa hpc - Truong Dai hpc Can Tho, 28:15-20.
NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11/2018
KHOA HOC CdNG NGHE
THE INCIDENCE OF CANINE PARVOVIRAL ENTERIRIS IN NAM THUY VETERINARY CLINIC OF DONG THAP PROVINCE
Tran Van TTianh, Tran Ngoc Bich, Nguyen Thi Yen Mai, Keovongphet Fhuthavong, Nguyen Phuc Khanh Summaiy
The study was performed to determine the incidence of Carune Parvovirus (CPV) infection in dogs from 1 month to 12 months of age with bloody diarrhea at Nam Thuy Vetennary Clinic in Dong Tiiap province from January to May, 2018. CPV disease was determined using the CPV - Ag Rapid test kit (Bionote Company). The therapeutic efficacy of dogs with positive CPV Ag test results was evaluated. Dogs were monitored for disease such as hypothyroidism, dehydration, diarrhea, blood toss, fecal status, and progression of the disease. The freatment was carried followed by drug administration, freatment period from 5 to 7 days. The treated efficacy was exarnmed through surviving dogs and their recovery. The result showed that the 13 out of 37 dogs were positive widi CPV antigen (35.14%). The proportion of infected dogs aged from 1 to 3 months of age had a liigher incidence rate than those from 6 to 12 months of age (61.54%
vs 7.69%) and there is statistically significant difference. The CPV disease was independent on breed and sex of dogs. The vaccinated dogs were infected with CPV lower than unvaccinated dogs (7.69% vs 92.31%).
The freated efficacy of CPV was 53.85%.
Keywords: Dog, diarrhea, infection rate, CPVAg test, treatment, Dong Thap.
Ngueri phan bien: PGS.TS. Le Van Nam Ngiy nhgnbli: 15/6/2018 Ngiy thong qua phin bi^n: 16/7/2018 Ngiy duy§t dang: 23/7/2018
82 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11/2018
