HOI NGHI KHOA HOC CONG NSHt: ailNn n y ^ .
CTNG DUHG ALPHA-AMYLASE TAI T G HQP BCN N H I E T S A N X U A T 0 VI^T , NAM De RO H 6 V A I T R O N G C O N G NGHIEP D^T
Tr»n Dinh Hin'-, NguyJn Qu6c V1ft\ Ng.iyln Th4 Trang', VQ Duy Luin=
' VHn Ctmg ngh^ sinh hoc. Vien Han iim Khoa hoc vi Cfing nghe Viet Nam
^ TOng cdng lyC6 phin dit may Nam Bjnh T6MTAT
TcT^lf2fZT^'Zs7r'^£s^P^^T,^y^'- B% ^"- ^ * •^ ^^ * - ' p^™"^ '^'•- -*» S
50°CvacuOnud70°C.
Ril hfi biig phuong phap tin trich, n ^ g dO a-amylase sir dung 1,2% d 50T trong I ^ d lupng d^^^^
a a ^ E iftfi h i IiO,l6% so v6i 0.15% khi su dung enzyme thuong m^i CA Wash 125. Ril hfi bang phuong ph^cu^n d. nfing d s T W l w e S d m j ! tniig be 14 5 g r t . V ep 1,6 kg/cm^ nhiet dO ii li 70^0, Ih&i gian li 6 gio. lu-jng tinh b$t cfinlp h«n vai khi sii
^ ^ a ^ S f C K X v S S o V . i l l s i dyng enzyme thuong m ^ C A W ^ ^
th^phuong phip cupn ii hnjng tinh bot c6n lai tren vii khi sii dimg a-amylasc tAi tfi hgp la 0.10% so vdi 0,15% khi su dpig enzymt thumg mai CA Wash 125. o l t lirgng vii sau rii hfi khi sii dyng a-amylasc td. to hop tfit hon khi su dyng e n ^ thuong m^i CA Wash 125. Cic chi tieu h6a ly cua nude thii rii hfi nhu pH, TOS, COD, BOD da dupc xic dinh cho thiy khi su dyng o-amylase til ij hpp th^p hon so vdi khi sii dyng enzyme thuang m^i CA Wash 125
Tir khoa: Alpha - amylase tai tfi hpp, Baallus subtilis 168M[pHV33BlamyBsPr], Nude thai ru hfi vii. rii hfi vii, Tfing c6ng ty Cj phin d^ may Nam Eijnh
M^TDAU
Alpha-amylase (a-amylase) hay c6n gpi Id Endo-1,4-a-D-glucan glucohydrolase c6 ky hi?u theo bang phSn log! enzyrne quAc t4 EC 3.2.1.1 \i enzyme thuy phSn ngSu nhiSn m6i lifin kdt 1,4-a-O-glucosidic nOi mgch cua polysao^iaride. Hbi h6t c6c a-amylase Id enzyme kim I09I din cd lon Ca^* d l hlnh thdnh cau tnJc th§ hifin hogt tfnh cung nhif a(l bfin. Phin ldn cdc a-amylase thuftc hp 13 (GHI 3) trong nh6m cac enzyme thuy phfin m6i lifin k^t glycosidic (Mobini-Dehkordi, Fahime, 2012, van der Maarel ef ai. 2002).
O'ng dyng enzyme trong cfing nghiSp dirge bit d^u ti> ndm 1894 khi Takamine sir dgng a-amylase tCr A. oryzae sin xuAt men tifiu h6a. VI Id enzyme thOy [rfidn phdn ti> tinh bftt thdnh cdc phdn 1i> nhfi co chi>a dan vj glucose nfin a- amylase dirge ung dung rfing rai trong cdc ngdnh cfing nghiep nhip che bien tinh bfit, ru h6 trong cfing nghifip d$t cflo chdt t4y n>a, sdn xu4t bdnh my, san xuSt gifiy, sdn xuit ni^u bia. trong y dirg^ va trong xCr i'^ mfii tnj*ng (Andey pf ak 2000, Kirk ef af., 2002, Mobini-Dehkordi, Javan. 2012). Thi tnromg tieu thg a-amylase b^n the gi(>i ndm 2004 khodng 2 ^ USD vd rnijc dfi tdng t i r ^ g hdng ndm khodng 3,3% (Monterio, Magalhaes, 2010).
Trong cfing nghifip dfit. tinh bfit d u ^ si> dgng dfi hfi hfia sgi ch6ng xa vd tao dfi hxrn, dp bin cho sg'i trong qud tinh dfit. Ngodi ra, sgi dugc hb tinh bfit chdng du^c hifin tu^ng ttch difin do ma sdt giira spi vd khung dfit dam bdo an to^
cho qud b'lnh dfit. Vdi sau khi dfit tnn>c khi du'a vdo tiy nhupm cin thlfit phdi rO ho ra khdi vdl. Tnjdc ddy, qud trlnh rH h i vdi th\rc hifin nhc' phirarng phdp axlL Tuy nhifin, vdi chgc nam trcr Igi day, phu'cmg phdp si> dung a-amylase trong rii h i vdi dd d u ^ thay ^ 1 hodn todn trang cfing nghi$p dfit vd du'Q'c coi Id bu'dc dfit phd trong cfing nghifit d$t (Hendriksen efaf., 1999, Kenealy et a/.. 2006; All ef af., 2010). Ngoai vi$c dat hi^u qud cao hong ru hfi, sO dgng a-amylase Idm giSm thilu cdc hda chit si> dgng nfin gidm fi nhilm moi tnnrng do nu'dc thai gdy ra (Feitkenhauer, 2003). Cdc kit qud nghien ci>u cho thiy, a-amylase ktidng dnh hu'dng tdi spi vdi.
Trtwig qud binh thgc hifin d l tdi 'Nghien ciiu sdn xuat enzyme a-amylase ben nhifit tai to hpp i>ng dgng trong cfing nghifip dfit* mfi sfi: BT.07.10/CNSHCB thuftc De dn phdt triln vd O'ng dgng cong nghfi sinh hoc bong ITnh vg'c cflng nghi^ chfi biln din nam 2020. a-amylase bin nhifit thu du'O'c ti> chung tai to hpp CTTH) Badllus sub&s 168MtpHV33BtamyBsPrl (Min TB ef ai. 2010) dd duvc iing dyng thdnh cfing d l ru h6 vdi tgi Tong cfing ty Co phin tW may Nam Bjnh.
N G U Y £ N L I ^ U V A PHlfONG P H A P Nguyen li$u
Enzwne CA Wash 125 (125 Ul/ml) Id a -amylase thu'ong mgi; Messour EX {chit din tham) dang si> dung tgi Tong caig ty Co phdn dfit may Nam 0|nh; enzyme a -amylase TTH ciia Vifin CNSH (120 Ul/ml);
Vdi mfic cd s l rna 3034 {Clu hue: 32 x 32/122 x 60; kilu dfit: van chdo; khi mpc: 160 cm; khi thdnh phim: 150 or;
bxjng iirgng mfic: 148 g/m^ nguyfin lifiu: 100% cotton; ty lfi Ifin h i : 12%; may dfit: Picanol.
C d c ^ a chil dCing trong xdc dinh COD, BOD5; dung dich Lugol.. Id loai tinh khilt dung thfing dgng trong phfing « PhiP0>ng phdp nghifin ci>u
RO h i vdi: Alpha-amylase bin nhifit thu dirpc tir chung TTH B. subtilis 168M[pHV33BlamyBsPr] da dupc Cmg dyng dt
)C CONG NGHE SINH HQC T O A N Q U 6 C 2013
rii h i vdi tai Ting cong ty C6 phin dfit may Nam Djnh. C h l phim a-amylase cd hogt dfi 100 - 120 Ul/ml tu'ang dgang d l l phim enzyme thuwng mgi CA Wash 125 dang si> dyng tgi Tong cdng ty C6 phan dfit may Nam Djnh dirge dira vdo rO ho theo 2 phiromg phap: tan trich vd cupn u.
Difdng khi> tgo thanh trong dung djch rij h i dirge xdc fljnh bdng phiremg phdp so mdu d 540 nm vdi Oiulc thi> DNSA.
Mlu vdi dirpc xdc <Snh trpng iirgng trudc vd sau khi rO d l tfnh todn (i/prra ho diroc ru ra khdi vdi theo ISO 3801-77.
Chit lirpTig vai bifdc va sau khi rO ho du'pc danh gid tai phfing KCS ciJa Tong cdng fe Co phin dfit may Nam Djnh theo ISO 13934(1 )-99.
Cdc chi tifiu hod IJ {pH. DO, dp due TDS) duvc do ngay tgi hifin bvdng bang mdy ProLab 2000 - SCHOTT {Di>c). BOD5 duvc xdc djnh bang may u va phdn tfch djnh luvng OXITOP - HACH 2173B (My); COD bang phirpng phdp dun hodn lu'u kin.
K £ T Q U A V A T H A O LUAN
Khdo sdt cac d i l u kifin sip dgng enzyme a-amylase b i n nhifit TTH rO h i vdi quy md phdng thi nghidm D l xdc djnh dnh hudng cOa ning dfi a-amylase TTH Ifin kha nang ru ho vdi. cac cdng thijc thi nghifim duvc trinh bdy d Bdng 1.
Bang 1. Cdng Ihvc ru ho vai trong ph6ng thi nghi$m dgt 1 H6a c h i t
Enzyme CA Wash 125 (ml) a -amylase 11 ri (mij Messour EX {ml) Vdi mfic icm)
M l u 1 (DC) 0,8 0.2 199,0 20-20
M i u 2 0.6 0.2 199.2 20*20
MSu 3 0,8 0,2 199,0 2 0 ^ 0
M l u 4 1.0 0.2 198,8 20*20
M l u 5 1,2 0.2 196,6 20*20 Phdn tich lirpng dud'ng tgo Ihdnh (Bdng 2) d l xdc dinh thdi gian thfch hpp ciio qud hinh ru ho. Vdi duvc sly khfi vd xdc djnh trpng luvng bifdc vd sau khi ru ho (Bdng 3) d l ddnh gid khd ndng hi ho vai cua enzyme.
Bang 2. H&m l i r a n g d i r d n g khi> t^o ra t r o n g djch r u h i (mgfml) ThM gian (h)
2 4 6 8
Bdng 3. Trpng l u v n g kh5 Trpng l i r ? n g vdi M l u vdi ban d i u M l u vdl sau khi hj Lijpng h i hao hyt
M l u l 1.14 1.18 1.19 1.39 cfia vdl tnedc vi
M l u 2 1,51 1,54 1,72 1.80 sau rQ h i (g) M l u 1
6,58 5,71 0,87
M l u 2 6,52 5,45 1,07
M l u 3 1.76 1.89 2,08 2.11
M l u 3 6,38 5,42 0,96
M l u 4 1,46 1.54 1.60 1,99
Mau 4 6,25 5,33 0,92
M l u 5 1,91 1.96 1,98 2,03
M l u 5 6.53 5.35 1.18 Kit qud thu du'pc cho thay, cdc mlu ti> s6 2 din sd 5 si> dgng enzyme TTH, lupng ho du'pc gidi phdng ra khdi vdi diu cao hon so vdi miu dOng enzyme thuang mgi dang dirpc si> dgng ^ i Tong cdng ty Co phan dfit may Nam Djnh.
Thi nghifim du'pc lap Igi vdl cdc mlu vdi cd kich thu-dc ldn hpn (160x40 an). Vdi du'pc ngdm frong hin hpp dung dich theo cfing thiJc mlu d Bdng 4 vd u d 70''C.
Bdng 4. Cong thOc xir ly rQ h i val trong ph6ng ttii nghidm dp't 2
H6a c h i t M l u l b (DC) M i u 2b M l u 3b CAWash 125 (ml) 2 - -
a-amylase (ml) - 2 2.5 Messour EX (ml) 0.5 0,5 0.5 H20(ml) 497,5 497.5 497,0 V i i mftc (cm) 160'40 160*40 160*40 Lly mlu xdc djnh dudng khu tgo thdnh trong qud trlnh ru h i d trudc va sau khi ru ho (8 gid). Vdi sau khi da ru h i dupc sly khfi vd xdc djnh b'png lu'png trudc vd sau khi rO d l ddnh gid kha ndng rQ hfi vai cda enzyme (Bang 5).
Bang 5. Lu'O'ng diro-ng khu' t^o thdnh vd lupng h i dirpc rQ kh6l vdl bdi 2 loai enzyme si> dung
l b 2.b 3 b
M l u
Trpng l u p n g Tnrdc TU h i
96.53 103.02 106,98
vai (g) Sau rO h i
88.37 94.57 98.27
L i r p n g d u d n g khi> t^o ra (mg/ml T r u d c rii ho
0,16 0,79 1.42
Sau ru ho 1.51 2.60 3.12
L u v n g h i d u p c rO khdi vai (g)
8.16 8.45 8.71 Kit qud thu duvc cho thiy, cdc mlu 2b vd 3b si> dyng enzyme TTH thu duoc luvng h i dupc giai phong ra khdi vdl diu
^J^fNGHTKHOAHQCcSFlGTlGFiE
« «i htm so vdi miu dung enzyme thuang mgi dang dirpc sir dgng tgi Tfing cdng ty C6 phin d$t may Nam Djnh (n^
m S h g T c d m ^ n ^ l u ^ t r u d c vd sau khi rO h i cho thiy. m l u sir dgng enzyme thuong mgi vd o-amiriase^
cho chit lupng vdi tucmg bf nhu nhau. ~ Sir dvng enzyme o-amylase b i n nhift TTH rfl h i vdi d quy mo sdn x u i t thfr nghi$m (100 kg vdl/me)
Qd trinh ro hd theo phirong phip cudn 0' Vii mgc dupc cho "9^11 enzyme vd hda chit Kng ngim (Mescour EX) ( ^ S f t n T * dp s S 1 bar u d 70°C bting 6 gld sau dd dua ra gilt d 90°C. Ty lfi e r i z ^ ^
hifin dupc binh bdy d Bdng 6.
O u , trinh riihd theo phuong phip tin trich (Ngim vii trong dung didi enzy"^e): V a l m f i c d u p c c*io n g d m b o n g d u n g * * e ™ e \ ^ h d a c h i t tdng n ^ (Mescour EX) d 50°C trong 1 g i d sau d d d u a ra gtgt d 9 0 C . T y 1$ enzyme, hfia chltsii durw v d k i t qud rG h i d u p c trinh bdy d Bang 6. So sdnh ket q u d ru h i theo hai q u y t r i n h b « n v d i 2 e n z y m e (a-arnitet T T H v d CA W a s h 125) cho thay, t J l f i h i cdn lai b i n vai khi s i l dgng a - a m y l a s e T T H t h i p h u n s o v d i CA Wash m Phucmg phdp rO h i cufin u t u o n g d u o n g v d i p h u a n g phdp t | n trfch n h u n g l u p n g e n z y m e sCr d y n g t h i p h a n vd thvt hifin d l ddng h a n nen d u p c chpn d l rii h i d quy mfi I d n .
Bdng 6. Ty I f c i c h6a c h i t sir dung v4 ty I f riJ h i ciia 2 enzyme si> dung theo hai phuo'ng phdp khdc nhau P h u w i g phdp cufln i i P h u o w g p h i p tan trich Thfing s i k j t t i u f t
a-amylase TTH C A W a s h 125 a-amylase TTH C A W a s h 125 Enzyme
Mescour EX P(6p)
Modul {T$ lfi gtCra vdi vd dd) Nhift d9
Thdi glan ii Nhift 00 gidt K/hrpng sau rO t i l T9 I f h i cdn lai trdn v i i
"C g/m'
Si> d g n g e n z y m e a - a m y l a s e b i n n h i $ t T T H rQ h i v d l d q u y m d s d n x u i t (1.000-2.000 k g v a l / m e j
Vdi mfic d u p c rO h i bfin ddy c h u y i n cfing ngh§ cfi s i n cGa T i n g cdng ty g i m cdc b u d c s a u : vdi mpc - • bd khdu - • di Ifing, n g i m enzyme n l u t i y . i i -* gidt - • tdy. T$ lfi enzyme, hda c h i t sO dgng v d c h l d f i t h u c hifin b'lnh bdy d Bdng 7.
Bdng 7. C i c thfing s l k } thu^t rfi h i v i i b i n g a-amylase TTH v i CA Wash 125 t^i T i n g cfing t y C l p h i n d i t may Nam Djnh QTCH Thfing s l k9 t h u f t Don vf a-amylase TTH CA Wash 125
Gift
S l m l f n g l i > a V f n t l c Enzyme Mescour EX P i p T h d l ^ a n u P i p V f n t i c ThM gland Nhift d f LBOX1 N h l f t d f b l 1:2:3 NhW d f b l 4:5:6 P i p V f n t i c Ttidi glan d Nhift d f I B O X I I Nhift d f b l 1 Nhift d f b l 4:5:6
s l m/p g/l kg/cm^
gid kg/cm' m/p phiM
"C
" c m/p phOt
"C 2 70 5 1.6 5 1 30 15 85:80:75 85;80;75 30 15
85;80;75
2 70 5
5
85;80;75
»
30 60
'i
B i n g 8. C i c chl tlAu c h i t l u p n g cua v i i m f c 3034 sau rQ h i
K h l i k/png vdi sau ru h i jglm']
Phirang p h i p thi>
H i m luyng h i cfin lgi (%)
D f b i t m l u lot cua vdl sau ru h i Thulc thd Lugon
B f i b i n dirt bdng vdi mfic (N) Dd ben ddt bdng vdl sau rO h i (N)
ISO 13934(1 )-99
Dpc = 326,30 Ngang = 174.12
DQC = 326.30 Ngang = 174,12 Doc = 287.14
Ngang = 156,48
DQC = 293.67 Ngang = 156,70 P h d n t i c h c h i t l u v n g s d n p h i m v d l 6wgc r u h o
Vdi s a u khi ru h i , g i ^ t t i y v d s l y khfi d u p c d d n h gid cdc c*il tifiu c h i t l u p n g tgi bp phdn K C S t h u ^ c p h d n g ky thugt c u a T i n g c o n g t y C 6 p h i n dfit m a y N a m D j n h (Bang 8).
MOt s 6 h i n h d n h thgc hifin ru h i tgi C f i n g t y C o p h d n dfit n h u f i m , Tong c f i n g ty C o phan d e l m a y N a m Dinh d u p c trinh bdy d H l n h 1 . D a t i l n h d n h 5 m l rii h i b a n g a - a m y l a s e T T H c h o k i t q u d I n djnh c h o p h e p khang djnh a - a m y l a s e T T H ttiu d u p c da tifiu c h u i n d l s u d g n g b o n g c d n g nghifip dfiL
P h d n tich c d m q u a n v d i m f i c 3 0 3 4 v d v d i s a u ru ho d i o t h i y , c d c c h l tilu c u a vai ru hfi bdng a - a m y l a s e T T H d g t c h i tieu c h i t l u p n g t h e o tifiu c h u i n Vifit N a m v d tuang d u a n g so v d i e n z y m e t h u o n g m a i hifin d a n g si> d g n g tgi T i n g cdng t y C o p h a n dfit m a y N a m D j n h .
Val sau khi q u i n 16 vd ii trong 6 gid Vai mpc 3034 sau khi 0 v i tnrdc gi^t Val sau khi rO h i vd gift Hlnh 1 . RQ ho vdi d q u y m d cfing nghiep tai Cong t y C6 p h i n d ^ n h u f m , T i n g cfing t y Co phan d f t may Nam Djnh P h a n t i c h c d c c h i t i e u h d a 1^ n u d e rQ h o
Tien h a n h s o n g s o n g si> dgng c i i n g mpt l u p n g e n z y m e 5 g/l d 2 lfi: Lfi dfii d i i r n g : si> d g n g e n z y m e t h u a n g - m g i C A W a s h 125 T o n g c f i n g ty m u a ; lfi t h i n g h i f i m : s u dgng e n z y m e a-amylase T T H b i n nhifit Vifin C N S H . M l u n u d e l l y n h u s a u : m l u n u d e h i r u , m l u b l xd 1 v d m i u b e xd 3 d u p c l l y vd do cdc c h i so tgi cho; C O D va BOD5 d u p c phdn t i c h theo T C V N lgi p h d n g thi nghifim cOa Vifin Hfia hpc, Vifin Hdn ldm Khoa hpc v d CQng n g h f i Vifit N a m K i t q u d p h d n tich m l u n u d e trlnh b d y d Bdng 9.
B i n g 9. Mgt s l c h l tifiu hfia 1]^ n u d e thai ru ho vai bang hai lo^i enzyme vjtri
l i y m i u B l ru h i B l x d l B l x a 3
L l n l l y m l u 1 2 1 2 1 2 3
pH 6.7 6.7 6.7 6.6 6.7 6.7 6.5 6.5
N u d e thdi ru ho TDS tq/l) 15,01 15,23 15,91 16.06 16,09 15,67 16,65 15,32 15.96
BOD5 (mg/l) 11.2 11.4 12,3 12,6 12,8 12.1 12,2 12,1 12,9
vai bang COD (mg/l) 17.5 17.9 16,2 16,9 16,7 16,8 16,4 17,1 17,3
C A W a s h 125 DO (mg/l) 7,02 6,96 6.75 6.72 6.90 6.65 6.78 6.78 6.68
IN
(mqfl) 2,36 2,64 2,36 2,45 2,98 2,23 2,40 1,97 2,06
ZP (mgfl)
0.036 0.042 0.162 0.198 0.199 0.163 0.192 0.283 0.376
pH 6.3 6.3 6.0 6.2 6.3 6.5 6,3 6,5
N u d e thai ru ho vdi bang a-amylase TTI TDS
(fl/l) 13.12 13.26 13.02 13,36 13.12 13.75 13,21 13,57
BODs (mq/1) 9.6 9.8 9.1 9.9 9.2 9.9 9,2 9,7
COD (mg/l) 12.1 12.9 13.3 13,9 13,3 14,0 13,4 13.5
DO (mofl)
7.12 7.06 7,11 7.11 7.28 7.02 7.09 7,08
I N (mq/l)
1,68 1.72 1.52 1.62 1.95 1,96 1.60 1,92
I P (mflfl) 0.011 0.024 0.010 0.072 0.027 0,037 0,071 0.025 Kfit q u d d B d n g 9 c h o t h i y , cdc c h i tieu h d a ly t h i p h p n cdc c h i tifiu d lfl doi chO'ng. D i e u ndy c d t h i gidl thich do a -
H O I N G H j K H O A H Q C U U N U N i ^ n i ; O I N H H Q C T O A f e q
amylase TTH d u p c s u dgng tnrc t i e p c h u a cho cac c h i t b d o q u a n vdo chfi p h i m b o n g k h i e n z y m e t h u a n g mgi di cdc c h i t bdo qudn.
K £ T L U I I I N
Enzyme a-amylase TTH ben nhiet b> c h f l n g Bacj7/us subWis 1 6 8 M [ p H V 3 3 B l a m y B s P r l d d d u p c u n g d g n g trong rO h l ^ i d quv mfi phfing thi nghiem vd quy m d cfing nghiep (tCp 100 d i n 2 0 0 0 k g / m l ) bang 2 p h u a n g p h d p t f i n trich v d cufn i T Cdc chi s i v l khd ndng ru ho vai, cdc chi tifiu hda iy c u a n u d e thai rn^ho b d n g a-amylase T T H b e n nhifit tuomg duoi^
vd t i t h a n so v d i e n z ^ e t h u a n g mai d a n g s u dgng tgi tdng c f i n g t y C o p h i n dfit m a y N a m D i n h .
LAicimon | Cong Irink dim Aifc hm bang kink phi cua De tdi "Nghien am sdn xudt enzyme a-amyiase ben nhiet tdildhf^Ang dvng Irong c6ng ngh^ dpil
ma s6 DT 07.I0/CNSHCB Ihuoc Di an phdt tnen vd ung difng cong nghe smh hpe Irong ITnh vuc cdng ngMfp che biin den nam 2020 \
T A I u e u T H A M K H A O
Andey A, Nigam P. Soccol CfK, Soccol VT, Singh D. Mohan R (2000). /Advances in miaohial amylases. Bidechnol Apd Srac/iem 31' 135-152.
Feitkenhauer H (2003). AnaertAic digestion of desizing waste water influence of pretreatment and anionic surfactant on degradOxi and Intemiediate accumulation. Enzyme Microb Technol 33:250-258
Haq I Wi, S Javed, MM Hameed. U Saleem, A Adnanf-Qadeer M A (2010). Production of alpha am^ase from a randomly inducsl mutant strain of badllus amyloiqefaciens and its application as a desizer in textile industry. Pek J Bd 42(1): 473-484. J Hendnksen HV, Pedersen S, Bisganl-Frantzen H (1999) A process for textile warp sizing using enzymeatKaily modified starches.
Patent ApplksAkm WO 99/35325.
Kirk 0 , Borchert TV, Fugisang CC (2002). Industrial enzyme applications. Curr Opin ^otechnd 13:345-351.
Mobini-Dehkordi M, Javan FA (2012). Applh:atk)n of alpha-amylase in biotechnology. J B / o / t o d a / s w o r f d 1(1)' 39-50 Monterk) P. Magalhies PO (2010) /application of microbial a-amylase in industry - A review. Braz J of Micmblology 41(4): 1-15.
wan der Maarel MJ, van der Veen B; Uitdehaag JC. Leemhuis H; Dijkhuizen L (2002). Properties and applications of slarch-convertin£
enzymes of the alpha-amylase family. J S/brec/)nof 94:137-155.
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T i l n Dinh M i n . Nguyftn Kim Thoa, Nguygn T h i Trang. Nguyln Q u i c Vifit Nguyln Thj Od (2010), Tach dong vd b i l u hifin gen a amylase bdn nhifit Irong Bacmis subWis 1 BSM. H^i nghj Khoa hgc M nl$m 35 nSm Wen Khoa hgc vi Cdng ngh$ mt Nam. Nxb Kha hpc tv nhiin v i Cfing ngh§: 330-339. v- r f v -
APPLYING OF THE RECOMBINANT THERMOSTABLE a-AMYLASE PRODUCEC IN VIETNAM FOR CLOTH DESIZING IN TEXTILE INDUSTRY
T r a n D i n h M a n \ N g u y e n Q u o c V i e t \ N g u y e n The T r a n g \ V u D u y L u a n ^ '^Institded8k)tochndogy. VAST
Nam Dinh TexBIg and Garment Jomt Stock Company
S U M M A R Y
OUITIlTinKI !
d 2 7 i l ! ! T S ! ™ V " T ? S , ' ' " ^ " ^ ^^ recombinant strain Bacillus subtilis 168M[pHV33BlamyBsPr] was applied for cloon u Z l w W c h - ^ i m i t I ^ " ' ' ^°^' ^"'='' '^^"•P^^y' • " ' ^ «""nbi„ant a-amylasc activity was prcpaW at 100-120^
The Temaining starch m the cloth ».
0.1% and 0.15%, respectively in the J , * ^ " ^ - !'^'^'"^*P**l"igofr method using of recombinant a-amylase and CA Wash 125 «m b y m l l i n g r f l t S i S t o g ^ i r c l o l h « T " 5 J ™ ™ : r ' ' ' ' ° • = " " " ' » « " «• ™°C in 1 h. Simililarity, result of cloth d e s i « stareh m clod, s«nples iere 0.15% and 0 20% i f j " ' ^ v """"»•"",»»• P " ' « k g ' ™ ' " « houn dedicated dtat the remaaaag the industdal scale desizing of 2000 k . ciod, bl „ o ^ 1"°' " T " ^ " " " " * " " ' " »«npl«. respectively. Moreover,, were 0.10% and 0,15% reieesZ We otalhv o f T T , ° r > ' ^ " ' ' <^* * ' " " ' " • *= " " ^ ' " S " " = ' ' " «'»* " " ' ' ° Wash 125. Tlte chnactetisd^of w i t e ^ r £ eW?H ° " ° * ° " ' " ' ™ ° ° " ' i ™ ' " - " " y l ^ ^ b»»er than enzyme CA
«ie water irom cloth desizing usmg both enzymes such as pH, TDS. COD, BOD. were analyzed.
ATeyHwnfa'BaciWut nifcnVu 168M[pHV33BlamvnsPri ,.1 ,1. J . - . ,
desizing, recombinant a-amytase. - ^ y ^ ' l , cloth desizing. Nam Dinh Textile and Garment JSC, Waste water from clodi
• Author for corrasspondenc TM, •84^-37567103; Email: idmaneibl.nc.vn
