Phin II: CdNG NGHg VI SINH 221
SAN XUAT VA THrONG MAI HOA CAC SAN PHAM SINH HOC DUNG TRONG NUOI TRONG THUY SAN
V5 Thj Hanh, Le Thi Bich Phirgng, Le Tan Himg, Truorng Thi Hong Van, Tran Thanh Phong
Phong Vi sinh ung dung, Vi?n Sinh hgc Nhi?t ddi
MdDAU
Nam 2006, nganh thuy san hudng tdi muc tieu xuit khiu 2,8 ty USD. Dien tich nudi frdng thuy sdn tham canh vd bdn tham canh d nude ta dang gia tdng nhanh, sy d nhiem mdi tiirdng nude ao nudi tdm do lugng tiiirc dn thira, chit thdi hiru co cdng ngdy
;cang nghiem frgng dnh hudng din ndng suit vd chit lugng nudi frdng, tdng khd nang Jnhiem benh cho tdm.
' ' ' / i'
; Khang sinh vd hda chat dugc sir dung nhieu trong phdng tri benh cho cdc ddi tugng thuy sdn nudi trdng se gay ra hien tugng "Idn thudc" vd sy ton du khdng sinh trong cdc mat hdng che bien Id rdo cdn cho viec xuit khau cdc sdn phim nay.
Mdt giai phdp da dugc chap nhan rgng rai fren the gidi Id sir dung cdc chl phim probiotics chiia cdc vi sinh vat sdng hiru ich cd khd ndng ldm sach mdi trudng nude ao nudi tdm, dieu chinh pH, gidm sd lugmg vi khuan gay benh cho tdm. Tren thi trudng hien cd nhieu che pham probiotics ciia nude ngodi dimg de xir ly ao nudi tdm dugc ddng bao tai Viet Nam cd gia thdnh cao.
Tir thyc tl fren, chiing tdi dd nghien cim sdn xuit va thir nghiem cdc che pham sinh hoc probiotics nhu BIOH, VEM vd PB fren cdc ao nudi tdm sii d cdc tiiih miln Nam.
Tir kit qua dat dugc, chiing tdi manh dan kit hgp vdi cdc cdng ty sdn xuit thudc tiiii y va nudi frong tiiiiy sdn de dua cdc chl phim ndy ra thi trudng vdi ten thuong mai cua cac cdng ty.
\AJ: LIEU VA PHU'OfNG PHAP
Giong vi sinh vat: Cdc chiing Bacillus spp., Lactobacillus spp., Saccharomyces spp., Rhodopseudomonas, Rhodospirillum dugc phan lap vd chgn Igc tai Viet Nam Thiet bi: Ndi len men (fermenter) 100 lit, may ly tam sieu tdc 20,000 vdng/phut, may siy phun, mdy ddng khd, Tu siy tinh, mdy xay, mdy trdn sieu tdc KBC-ST- 20, tii lanh am...
M6i tnrdng (MT) san xuat che pham BIOII va PB: MT djch chiet ngii cdc diing sdn xuit sinh khdi vi khuin Bacillus spp; MT MRS dimg de tiiu sinh khdi vi khuin Lactobacillus spp; MT Malt - Cao nim meri dimg de tiiu smh khdi Saccharomyces spp;
Moi tnrdng Norbert Pfennig dimg dl nudi ciy vi khuan quang dudng.
222 HOI nghj KHOA HQC VA CdNG NGHg 2007
San xuat a-amylase va protease: tii vi khuin Bacillus subtilis bang phuong phap len men ban ran.
San xuat che pham VEM: Cai tiln qui tiinh san xuit EM (Effective Microorganisms) cua giao su Temo Higa, Nhat Ban dh sdn xuit VEM cd mat do vi sinh vat hihi ich:
Bacillus spp., Lactobacillus spp., Saccharomyces spp. Va vi khuin quang hgp de tao ra che pham VEM.
San xuat che pham PB: Nudi ciy vi khuin Rhodopseudomonas vd Rhodospirillum
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trong mdi trudng Norbert Pfennig dudi dnh sang mat trdi, sau 2 tuan nudi cay, dem ddng chai va bdo qudn trong tdi.
Xac dinh cac chi tieu sinh hoa: Mat do vi sinh vat bdng phuong phap dem khuin lac; boat luc a-amylase bdng phuong phdp Smith va Roe; hoat lyc enzym protease bang phuang phdp Anson cdi tiln; Dinh lugng NH3-N, H2S, NO2-N bang phuang phap so mau.
Thir nghiem hi«u qua che pham BIOII tren ao nuoi t6m: Tren ao tom sii 45 ngdy tudi tai cdc ao nudi tdm bdn tham canh d tmh Ba Ria-Viing Tau vdi ndng dg 0,2-0,5 ppm. Sii dung che pham BRF2 cua My cho ao ddi chung vdi ndng dg 0,5 ppm. Theo doi cac chi tieu nhu mau nude, pH, NH3-N, H2S, NO2-N vd mat do vi khuin Vibrio frong nude ao.
Thir nghiem d cdc tinh Kien Giang, Tiln Giang vdi lilu lugng lOOg/lOOOm^/tuan, Thu- nghiem hieu qua che pham VEM tren ao nuoi torn: Chl phim VEM dugc thir nghiem tren cdc ao nudi tdm tai Bd Ria-Viing Tau; theo ddi trgng lugng trung , binh, ti le sdng va he sd FCR (food conversion ratio), ddi chung la san phim Pond-Clear.
KET QUA VA THAO LUAN
i
Che pham probiotics BIOH ;
Bang 3.1: Thdnh phdn c h l p h i m probiotics BIOII
Th^nh phan Bjnh lu^ng
Lactobacillus spp. IO' CFU/g Bacillus spp. 10^° CFU/g Sacchromyces spp. 10^ CFU/g a-amylase 2000 Ul/g Protease 20 Ul/g
ph^n II: CdNG NGHg VI SINH
223
Ket qua thir nghiem che pham BIOH tren ao nuoi torn
Bdng 3.2. Thip nghifm tai Bd Rja - Vung Tdu
Ciic d9^ xu
Th6n gian (ng
O »
o o 3;
0 c 3- o-
NH3-N (mg/l)
N O r N (mg/l)
H2S (mg/l)
Vibrio (CFU/ml)
pH
ly
Sy)
BIOII BRF2 BIOII BRF2 BIOII BRF2 BIOII BRF2 BIOII BRF2
Xu- li dqyt 1 (BIOII 0,2ppm;
BRF20,5ppm)
0 3 1.3 1.12 0.63 0.53 0.074 0.126 0.001 0.002 0.023 0.013 0.031 0.023 6083 560 430 295
•
Xi> li dpi 2 (BIOII O.Sppm;
BRF2 O.Sppm)
6 9 12 1.22 0.6 1.3 0.35 1.40 0.90 0.098 0.008 0.045 0.005 0.000 0.001 0.013 0.051 0.016 0.020 0.067 0.005 340 1140 2000 300 1350 1300
Xu- li dqrt 3 (BIOII O.Sppm;
BRF2 O.Sppm)
15 18 21 0.95 0.85 1.45 0.95 0.45 1.50 0.000 0.000 0.000 0.000 0.000 0.0005 0.049 0.023 0.011 0.002 0,001 0.002 700 450 270 1000 500 400 7.3-8.5
7.6-8.2
Xi> li d 9 t 4 (BIOII O.Sppm;
BRF2 O.Sppm)
24 27 30 0.45 0.75 0.70 0.90 0.75 0.72 0.000 0.005 0.002 0.002 0.002 0.001 0.013 0.011 0.007 0.002 0.002 0.002 300 300 200 300 200 200
Khi tang nong do BIOII tir 0,2 ppm len 0,5 ppm d cac dgt xir li thii 2, 3 vd 4, nong dp NH3-N, NO2-N, H2S sau 30 ngay gidm xudng d miic cho phep. Cdc chi tieu pH, COD dn dinh, thich hgp cho sy phat triln ciia tdm. Mat do Vibrio gidm cdn 200 CPU/
ml bdng vdi ao ddi chiing xir li vdi BRF2 sau 30 ngay.
Bdng 3.3. K i t qud thiF nghi?m c h l phim BIOII (100 g/1000 mVtuin) tren ao torn t^i Kien Giang, Tiln Giang
Cic chl tigu Chit lu'ang nuifc pH
Ti 1$ b$nh NSng suit (tin/ha) Nang suit tang (%)
Kien Giang Thi> nghl$m
T6t, khdng c6 mui
7 , 8 - 8 , 1
Kh6ng dang kh
7,8 121.9
h6l
D6i chi>ng x i u , c6 miii
8 , 5 - 9 , 3 1 5 - 2 0 % 6,4 100
hai
Tl4n ThO' nghl$m
Giang Mau nu'(5'c, d() trong du-^c c3l thi$n
7 , 5 - 8 , 3
Kh6ng dang k i
5,7 119,1
06i chung x i u , c6 mill hdi
7 , 5 - 9 , 5 1 5 % 4,7 100
Bang 3.3 cho thay: Nang suat tom binh quan tang tir 19,1%- 21,9%
Che pham VEM
Bdng 3.4. Thdnh phin vi sinh v?t trong c h l phim VEIVI
Vi sinh vat Chi phim VEM
Vi khuin lactic Vl khuin Bacillus spp Vi khuin quang du^ng Nim men
1x10'CFU/ml 1x10'° CFU/ml IxlO'CFU/ml 1x10'CFU/ml
224 Hai nqhj KHOA HQC VA CdNG NGHg 2007
Thir nghiem hieu qua che pham V E M tren ao nuoi torn
Bang 3.5. K i t qua thu' nghifm c h l p h i m VEM tren cac ao nuoi torn tal Bd Rja-Vung Tdu
Chi tieu Ti 1$ s6ng (%)
Trpng iu'Q'ng trung binh (g/con) Kich cff t6m (S6 con/kg) San lu'i?ng binh quSn (kg/ha) T6ng lu'p'ng thifc an (kg) Ha s6 FCR
Pond-Clear 63,96
28,2 35,5 4.545 3.502 1,75
VEM
62,73 28,9 34,5 4.546 2.671 1,78
Bang 3.5 cho thiy: trgng lugng trung binh vd ti le sdng cua tdm thu hoach d hai ao sir dung chl phim VEM khdng khdc biet nhilu so vdi ao sir d\mg san phim Pond-Clear.
Che pham PB
Qua thir nghiem che pham PB tren cac ao nudi tdm sii, ket qua cho thay cd tac dung phan giai thuc an du thira vd chat thai tich tu dudi day ao, giam BOD, COD, dn dinh chat lugng nude, giiip can bang sinh thai, gidm ti le benh cho tdm.
Thirong mai hoa cac che pham diing trong nuoi trong thiiy san
Chiing tdi da ket hgp vdi cdc cdng ty thudc thii y va nudi trdng thuy san de thuong mai hda che pham BIOII va VEM mang thuong hieu ciia cac cdng ty.
Bang 3.6. Thu'vng m^i hda c h l p h i m BIOI, BIOII vd VEM
STT 1 2 3 4 5 6 7 7 8 9 10 11 12 13
Ten s i n phim NAVET Biozym Enzym subtilis Enzymmin subtilis Biolactlzym NP-Lactlzym L-AKL B-AKL BACILLUS MAZAR BS@
BACTERI LASA BIOLAS-2 BIOLAS- Pb
KET LUAN
Ngu6n BIOII
g6c Bacillus subtitis Bacillus subtitis BIOII
BIOII BIOII BIOII
Bacillus subtitis VEM
VEM Pb VEM BIOII VEM
Tdm, ca T6m, ca Tdm, ca Tam, ca Tam, ca Tam, ca, Tam, ca.
Tom, ca Tam, ca Tam, ca Tam, ca Tam, ca Tam Tam
£)6i lu'ang
(rwj'ifc ki4m) (nya-c phSn)
Cong ty sdn xuit Thu6c thii y Trung u-ang Thu6c thu y Sai Gdn Thu6c Thu y Long An II Thu6c thu y NAPHA CP NTTS Khanh Long Dat Viet Co.LTD
Ogi Tru'6'ng Sen
Che pham BIOII, PB vd VEM cd hieu qua frong viec lam sach mdi trudng nude ao nudi tdm, can bdng pH, giam sd lugng vi khuin gay benh cho tdm. Nang suit tdm biob
Phin II: CdNG NGHg VI SINH 2 2 5
quan thu hoach tang 19,1% - 21,9% so vdi san phim BRF2, he sd FCR thip han so vdi san phim Pond-Clear, trong khi gid thdnh ciia che pham BIOII thip ban gin 10 lin.
Chiing tdi da ket hgp vdi cac cdng ty thudc thii y va nudi trdng thiiy sdn dk thuong mai hda chl phim BIOII vd VEM mang thuong hieu cua cdc cdng ty.
TAI LIEU THAM KHAO
1, Havenaar, R., Ten Brisk, B., Selection of strains for probiotic use. In: R. Fuller (ed.), Probiotics the scientific basic. Chapman and Hall, London. P. 209-224, 1992.
2. Vd Thi Hanh va cs (2003). Nghien ciiu sdn xuat che pham hdn hgp vi sinh vat sdng vd enzyme tieu hda dimg trong chan nudi vd nudi trdng thiiy sdn. Bdo cdo nghiem thu dl tai, Sd Khoa hgc vd Cdng nghe TPHCM
SUMMARY
Production and commercialize the bio-products using for aquaculture
Vo T Hanh, Le Bich Phuong, Tran Thanh Phong, Le Tan Hung, Truong T Hong Van Institute of Tropical Biology
The bioproducts such as BIOII, VEM and PB have been shidying and producing;
these bioproducts have been using for aquaculture effectively. The BIOII and VEM had effect on cleaning water, pH stability, reducing pathogenic bateria on shrimp. The average yield increased 19,1% - 21,9% compared with BRF2 products, FCR (food conversion ratio) lowered compared with Pond-Clear product, meanwhile, price of BIOII lower 10 times BRF2.
