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A study of changes in the thickness and chemical composition of the skin of sheep during growth and after shearing : being a thesis presented in partial fulfillment of the requirements for the degree of M. Agr. Sc., Massey Agricultural College, University of New Zealand

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A STUDY OF CHANGES IN THE THICKNESS AND CHEMICAL COMPOSITION OF THE SKIN OF SHEEP DURING GROWTH AND AFTER SHEARING

by

MARIA MANIKA WODZICKA

Being a thesis presented in partial fulfil1ment of the requirements for the

degree of M. Agr . Se.

Massey Agricultural College , Uni�ersity of New Zealand.

March , 1 95 4 .

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A C K N 0 W L E D G E M E N T S

I wi sh to expre s s my gratitude to members of

the Ruakura Animal Research Station and the Massey Agricultural College staffs for the help I have

re ceive d from them during this investigation.

Special thanks are due to my supervisors

Professor A.L. Rae and Dr. C.P. M cM e ekan; to

ld.r. W. G . Whitle stone , Mr. N.T. Clare, Mios P. Bartram,

Dr. J. Jam e s , Mr. F.R. Cockrem and

Mr. P .M.

Linklat er

for help and advice .

For technical assi stanc e I am ind ebted to Mrs. R. Holmberg, Mr. D. Soruervill e, Mr. J. K err ,

Mr. B. Miller, Mr. J . Gib s on and 1tr. B. Wright.

Thi s the sis was carried out und er the tenure of the Sh ell Scholarship.

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TABLE OF CONTEN T S

GEN ERAL INTRODUC TI ON

CHAP TER I - DEV ELOPMEN T OF TECHNIQUE S

A. SKIN THICKN ESS MEASUREMEN T

IN TRODUC TION I . M ETH OD S AND N� TER IALS (a) Ins truments

(i) Instrument for measurem ent

Page

1 4

4 5 5

of skin thi ckn s s 5 (ii) M e thods of making frozen

s e cti ons 8

(iii) Other in struments and

materials . 8

( b ) Expe rim ental Animals 9

II . NA TU RE AND SCOPE OF EXPERIMENTS . 9 (a) Prelim inary Experiment I 1 1 (1) Introdu c t i on 11 (2) P lan of Experiment 12 (3) Re sults 15 ( b ) Preliminary Experiment II 22

DISCU S SION SUMMARY

Intro duc tion 22 Plan of Experiment . 2 3 R e sults 2 3 Sampling procedure finally

adopted 26

27

29

(5)

iv . Pag e

B. CHEMICAL ANALYSIS 30

INTRODUCTION 30

I. METHODS AND MATERIALS 31

(a ) Appara tu s 31 (b) Sampling Procedu re and Analysis 32

II. NATURE AND SCOPE OF EXPERIMENTS 34

SUMMARY 44

CHAPTER II - DISTRIBUTION Ol SKIN THICKNESS

O VER THE BODY OF SHEEP 45

INTRODUCTION 45

I. METHO DS AND MATERIALS 45 ( a ) Ou tline of Experiment o 45 (b) Experimental Animals 46

(c) Technique

!!.RESULTS DISCUSSION

0

SUMMARY

. .. . .

46

47

47

49

CHAPTER III - THE SKIN DEVELOPMENT EXPERIMENT 50

INTRODUCTION

I. METHODS AND MATERI ALS

(a) Plan of Experiment (b) Experimental A»iaals

( c) Experim ental. Teolmique ( d ) Sampling Pr o ce dur e

50

50

50

52

58

59 \'

(6)

\,')

II. RESULTS

(a) Skin Thickne s s ( b) Chemi cal Composition

DISCUSSION

SUMMARY

CHAPTER IV - THE SHEARING EXPERIMENT

INTRODUCTION

I. METHODS AND MATERIALS

(a) Plan of Experiment ( b ) Experimental Animals

(c) Experimental Technique

II. RESULTS

(a) Skin Thickne s s ( b ) Chemi cal Composi ti on 0

DISCUSSION 0

SUMMARY 0

GENERAL SUMMARY AND DISCUSSION

v .

Page

60 60 7 1

81 85

86 86 86 86 89 89

90 90

93

94 96

97

Referensi

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No part of this thesis may be reproduced by any means without the permission of at least one of the copyright owners or the English Department, Faculty of

No part of this thesis may be reproduced by any means without the permission of at least one of the copyright owners or the English Department, Faculty of

No part of this thesis may be reproduced by any means without the permission of at least one of the copyright owners or the English Department, Faculty of