Efek Antibakteri Ekstrak Etanol Umbi Lobak (Raphanus sativus L.) terhadap Fusobacterium nucleatum ATCC 25586 sebagai Bahan Alternatif Medikamen Saluran Akar (Secara In Vitro)

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2. Athanassiadis B, Abbott PV, Walsh LJ. The use of calcium hydroxide, antibioticcs and biocides as antimicrobial medicaments in endodontics. Aust Dent J 2007; 52(1): 64-6.

3. Spratt DA, Pratten J, Wilson M, Gulabivala K. An in vitro evaluation of the antimicrobial efficacy of irrigants in biofilms on root canal isolates. Int End J 2001; 34: 300.

4. Kim D, Kim E. Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment. Restorative Dentistry and Endodontics Rev 2014: 1-4.

5. Haapasalo M, Qian W. Irrigants and intracanal medicaments.In:Ingle JI, Bakland LK, Baumgartner JC. Endodontics. 6thed. Hamilton:BC Decker Inc,2008: 1009.

6. Siquiera JF, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide. Int End J 1999; 32: 361-4.

7. El Karim I, Kennedy J, Hussey D. The antimicrobial effects of root canal irrigation and medication. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 103(4): 560-1, 564-5

8. Johnson WT, Noblett WC. Cleaning and shaping. In: Torabinejad M, Walton RE, Dolan J, Pendill J, editors. Endodontics principles and practice. 4th ed. Missouri: Saunders Elsevier, 2009: 263,279.

9. Gomes et al. In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms. Braz Dent J 2002; 13(3): 155. 10.Mustafa et al. Role of calcium hydroxide in endodontics.GJMEDPH 2012;

1(1): 53-4.

11.Gomes BPFA, Vianna ME, Zaia AA, Filho FJS. In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102(4): 545.


hydroxide on cultivable bacteria in infected root canals. J Endod 2007; 33(7): 800-4.

13.Nwaokorie et al. AP-PCR and antimicrobial susceptibility patterns of Fusobacterium nucleatum associated with chronic periodontitis among

patients at Lagos University Teaching Hospital. British Microbiol Res J 2012; 2(2): 98.

14.Boldstad AI, Jensen HB, Bakken V. Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum.Clin Microbiol Rev 1996; 9(1): 55-9, 64-5.

15.Kipalev et al. Detection of selected anaerobic pathogens in primary and secondary endodontic infections in a Turkish population. African J of Microbiol Res 2014; 8(13): 1462-5.

16.Guimaraes et al. Microbiological evaluation of infected root canals and their correlation with pain. RSBO 2012; 9(1): 32-5.

17.Sedgley CM. Virulence of endodontic bacterial pathogens. In:Fouad AF. Endodontic Microbiology. 1st ed. Iowa: Wiley Blackwell, 2009: 138-9.

18.Peciuline et al. Microorganisms in root canal infections. Stomatologija,Baltic Dent and Maxillofacial J 2008; 10(1): 4.

19.Pollanen MT, Paino A, Ihalin R. Enviromental stimuli shape biofilm formation and the virulence of periodontal pathogens. Int J Mol Sci 2013; 14:


20.Kanaparthy A, Kanaparthy R. Biofilms-the unforgiving film in dentistry (cliniccal endodontic biofilms). Dentistry 2012; 2(145): 3.

21.Svensater G, Chavez LP, Theilade E. The microbiology of the necrotic pulp. In: Bergenholtz G, Bindslev PH, Reit C. Textbook of endodontology. 2nd ed. Iowa:Wiley Blackwell, 2010: 95-7.

22.Singh et al. In vitro evaluation of effectiveness of chlorhexidine, Curcuma longa, calcium hydroxide as intracacal medicaments in Enterococcus faecalis


23.Pujar M, Makandar S. Herbal usage in endodontics. Int J Contemporary Dentistry 2011; 2(1): 35.

24.Sari LORK. Pemanfaatan obat tradisional dengan pertimbangan manfaat dan keamanannya. Majalah ilmu kefarmasian 2006; 3(1): 2.

25.Shukla et al. Antimicrobial efficacy of Raphanus sativus root juice. Int J Pharmacy and Pharmaceutical Science 2011; 3(5): 89-92.

26. Kumar N, Nautiyal S. An inventory of medicinal wealth of Jhil-Mil Jheel conservation reserve. Int J Herbal Medicine 2013; 1(2): 1.

27.Pal SK, Shukla Y. Herbal medicine : currrent status and the future. Asian Pacific Journal of Cancer Prevention 2003; 4: 281.

28.Amalia. Efektivitas daya antibakteri ekstrak etanol siwak (Salvadora persica L.) terhdap pertumbuhan Fusobacterium nucleatum sebagai alternatif

medikamen saluran akar (penelitianin-vitro). Medan: USU, 2013.

29.Lubis VD. Daya antibakteri ekstrak etanol daun Afrika (Vernonia amygdalina) sebagai bahan alternatif medikamen saluran akar terhadap

Fusobacterium nucleatum (penelitian in vitro). Medan: USU, 2014.

30.Beevi et al. Isothiocyanate profile and selective antibacterial activity of root, stem, and leaf extracts derived from Raphanus sativus L. Foodborne Pathogens and Disease 2009; 6(1): 131-6.

31.Shrivastava N, Bhargava R. Antibacterial potential of selected medicinal

plants against Escherichia coli dan Staphylococcus aureus. Acta Biologica India 2012; 1(1): 133-4.

32.Gutierrez RMP, Perez RL. Raphanus sativus (Radish) : their chemistry and biology. The Scientic World Journal 2004; 4: 811-37.

33.Singh P, Singh J. Medicinal and therapeutic utilies of Raphanus sativus. Int J Plant, Animal, and Enviromental Sciences 2013; 3(2): 103-5.


35.Janjua S, Shahid M, Abbas FI. Phytochemical analysis and in vitro antibacterial activity of root peel extract of Raphanus sativus L. var niger. Adv Med Plant Res 2013; 1(1): 1-7.

36.Ahmed N, Neelakantan P. Antiseptics and antibiotics used in regenerative endodontics. Int J Pharmaceutical and Clinical Research 2013; 5(4): 141. 37.Spanberg LSW, Haapasalo M. Rationale and efficacy of root canal

medicaments and root filling materials with emphasis on treatment outcome. Endodontic Topics 2002; 2: 47.

38.Wang CS, Debelian GJ, Teixeira FB. Effect of intracanal medicament on the sealing ability of root canals filled with resilon. J Endod 2006; 32(6): 532-36. 39.Metzger Z, Basrani B, Goodis HE. Instruments, materials and devices. In:

Hargreaves KM, Cohen Stephen, Berman LH, editors. Cohen’s pathway of the pulp. 10th ed. Mosby Elsevier, 2011: 253-5.

40.Siquiera JF. Strategies to treat infected root canals. J of The California Dent Association 2001; 29(12): 825-6.

41.Farhad et all. Evaluation of the antibacterial effect of calcium hydroxide in combination with three different vehicles : an in vitro study. Dent Res J 2012; 9(2): 167.

42.Estrela C, Holland R. Calcium hydroxide: study based on scientific evidences. J Appl Oral Sci 2003; 11(4): 274.

43.Porteiner et al. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int End J 2001; 34: 186-187.

44.De Moor RJG, De Witte AMJC. Periapical lesions accidentally filled with calcium hydroxide. Int End J 2002; 35: 946-58.

45.Hofstad T. The genus Fusobacterium. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E, editors. The prokaryotes vol 7. Springer Science Business Media,2006: 1018-23.


47.Siqueira JF, Rocas IN. Microbiology and treatment of endodontic infectionsIn: Hargreaves KM, Cohen Stephen, Berman LH, editors. Cohen’s pathway of the pulp. 10th ed. Mosby Elsevier, 2011: 561.

48.Teles et al. Microorganisms: the reason to perform endodontics in Microbial pathogens and strategies for combating them: science, technology, and education.Editor : Vilas AM. Formatex Research Center 2013: 1778-86. 49.Siqueira JF. Microbial causes of endodontic flare-ups. Int End J 2003; 36:


50.Siquiera JF. Treatment of endodontic infection. Germany: Quintessence Publishing Co.Ltd, 2011: 26.

51.Usha HL, Anjali K, Mehta D. Biofilm in endodontics: new understanding to an old problem. IJCD 2010; 1(3): 46.

52.Handal et al. Bacterial diversity in persistent periapical lesions on root-filled teeth. J of Oral Microbiol 2009; 1: 1-6.

53.Diaz PI, Zilm PS, Rogers AH. Fusobacterium nucleatum supports the growth

of Porphyromonas gingivalis in oxygenated and carbon dioxide depleted

enviroments. Microbiology 2002; 148: 470.

54.Savoia D. Plant derived antimicrobial compounds: alternatives to antibiotics. Future Microbiol 2012; 7(8): 982.

55.Ciocan ID, Bara II. Plant products as antimicrobial agents. Analele Ştiinţifice ale Universităţii, Alexandru Ioan Cuza, Secţiunea Genetică şi Biologie Moleculară 2007; 8: 151-5.

56.Lim SH, Darah I, Jain K. Antimicrobial activities of tannins extracted from Rhizophora apiculata barks. J of Tropical Forest Science 2006; 18(1): 59.

57.Ajayi IA, Ajibade O, Oderinde RA. Preliminary phytochemical analysis of some pant seeds. Res J Chem Sci 2011; 1(3): 58-60.

58.Soetan et al. Evaluation of the antimicrobial activity of saponins extract of Sorghum bicolor L. moench. African J of Biothecnology 2006; 5(23): 2405-7.


60.Souza SM, Monache FD, Smania A. Antibacterial activity of coumarins. Verlag der Zeitschrift fur Naturforschung 2005; 60: 693.

61.Cowan MM. Plant products as antimicrobial agents. Clinical Microbial Review 1999; 12(4): 564-573.

62.Cambau E, Guilard T. Antimicrobials that affect the synthesis and conformation of nucelic acids. Rev Sci Tech Off Int Epiz 2012; 31(1): 80-5. 63.Geweely NS. Novel inhibition of some pathogenic fungal and bacterial

species by new synthetic phytochemical coumarin derivatives. Annals of Microbiology 2009; 59(2): 359-368.

64.Alnajar et al. Acute toxicity evaluation, antibacterial, antioxidant, and immunomodulatory effects of Melastoma malabathricum. Molecules 2012; 17: 3547-8.

65.Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. Int J of Antimicrobial Agents 2005; 26: 348-53.

66.Stefanovic et al. Antibacterial activity of naturally occuring compounds from selected plants. Department of Biology and Ecology Faculty of Science University of Kragujevac Serbia, 2012.

67.Karou et al. Antibacterial activity of alkaloids from Sida acuta. African J of Biotechnology 2005; 4(12): 1452-7.

68.Enwa et al. Mechanisms of antimicrobial actions of phytochemicals against

enteric pathogens. J of Pharmaceutical, Chemical, and Biological Sciences 2014; 2(2): 77-85.

69.Sampurno, Ritiasa K, Muribat AR, dkk. Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan, 2000: 7-20.