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Pengaruh Pemberian Ekstrak N-Heksan Buah Andaliman (Zanthoxylum acanthopodium DC.) Terhadap Perkembangan Struktur Kraniofacial Fetus Mencit (Mus musculus L.) Strain DDW

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(1)

Lampiran A.1. Uji Statistik Jumlah Implantasi Mencit Perlakuan Ekstrak

N-heksan Buah Andaliman

Tests of Normality

.251 6 .200* .869 6 .223 Jumlah_Im plantasi

Statistic df Sig. Statistic df Sig. Kolmogorov-Sm irnova Shapiro-Wilk

This is a lower bound of the true s ignificance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

2.055 4 25 .117

1.484 4 25 .237

1.484 4 9.521 .282

1.853 4 25 .150

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Jumlah_Implantasi

Levene

Statistic df1 df2 Sig.

ANOV A

Jumlah_Implantasi

40.000 4 10.000 .563 .692

444.167 25 17.767

484.167 29 Between Groups

W ithin Groups Total

Sum of

Squares df Mean S quare F Sig.

Lampiran A.2. Uji Statistik Jumlah Embrio Resorb (%) Mencit Perlakuan

Ekstrak N-heksan Buah Andaliman

Tests of Normality

.194 6 .200* .914 6 .461

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true s ignificance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

65.295 4 25 .000

6.062 4 25 .001

6.062 4 5.022 .037

52.388 4 25 .000

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Embrio_Resorb

Levene

(2)

Kruskal-Wallis Test

Perlak uan K0

N Mean Rank

Te st S ta tisticsa,b 2.0 60

4 .72 5 Ch i-Sq uare

df

As ymp . Si g.

Em brio _ Re sorb

Kruska l W allis Tes t a.

Groupi ng V aria ble: Perl akua n b.

Lampiran A.3. Uji Statistik Jumlah Fetus Mati (%) Mencit Perlakuan Ekstrak

N-heksan Buah Andaliman

Tests of Normality

.492 6 .000 .496 6 .000

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

5.103 4 25 .004

.817 4 25 .526

.817 4 6.722 .554

3.432 4 25 .023

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Fetus_Mati

Levene

Statistic df1 df2 Sig.

Kruskal-Wallis Test

Ra nks Perlak uan

K0 Fetus_Mat i

(3)

Test Statistics

a,b

Kruskal Wallis Test

a.

Grouping Variable: Perlakuan

b.

Lampiran A.4. Uji Statistik Korpus Luteum Mencit Perlakuan Ekstrak

N-heksan Buah Andaliman

Tests of Normality

.249 6 .200* .891 6 .322 Korpus _Luteum

Statistic df Sig. Statistic df Sig. Kolmogorov-Sm irnova Shapiro-Wilk

This is a lower bound of the true s ignificance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

1.611 4 25 .203

1.311 4 25 .293

1.311 4 18.377 .303

1.607 4 25 .204

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Korpus _Luteum

Levene

Statistic df1 df2 Sig.

Kruskal-Wallis Test

Ra nks

Perlak uan

K0

Korpus _Luteum

N

Mean Rank

Te st S tatisticsa,b

1.832

Korpus _ Luteum

Kruskal W allis Tes t a.

(4)

Lampiran A.5. Uji Statistik Kehilangan Praimplantasi Mencit Perlakuan

Ekstrak N-heksan Buah Andaliman

Tests of Normality

.397 6 .004 .706 6 .007

.332 6 .037 .837 6 .122

.191 6 .200* .964 6 .852

.271 6 .190 .785 6 .043

.212 6 .200* .952 6 .758

Perlakuan K0 KP P1 P2 P3 Kehilngan_Praimplantasi

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true significance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

4.155 4 25 .010

1.087 4 25 .385

1.087 4 7.888 .425

3.026 4 25 .036

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Kehilngan_Praimplantas i

Levene

Statistic df1 df2 Sig.

Kruskal-Wallis Test

Ra nks

6 8.42 6 14.92 6 18.50 6 16.42 6 19.25 30

Perlak uan K0 KP P1 P2 P3 Total Kehilngan_Praimplantasi

N Mean Rank

Te st S tatistics

a,b

5.878

4

.208

Chi-Square

df

As ymp. Sig.

Kehilngan_

Praimplant asi

Kruskal W allis Tes t

a.

(5)

Lampiran B.1. Uji Statistik Berat Badan Induk (g) Mencit Perlakuan Ekstrak

N-heksan Buah Andaliman

Tests of Normality

.247 6 .200* .880 6 .268

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true s ignificance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

2.525 4 24 .067

1.814 4 24 .159

1.814 4 16.292 .175

2.588 4 24 .062

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Berat_Badan_Induk

Levene

Statistic df1 df2 Sig.

ANOV A

Berat_Badan_Induk

26.482 4 6.620 .677 .615

234.846 24 9.785 261.328 28

Between Groups W ithin Groups Total

Sum of

Squares df Mean S quare F Sig.

Lampiran B.2. Uji Statistik Jumlah Fetus Hidup Perlakuan Ekstrak N-heksan

Buah Andaliman

Tests of Normality

.135 6 .200* .990 6 .988

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true significance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

6.584 4 25 .001

5.302 4 25 .003

5.302 4 21.480 .004

6.560 4 25 .001

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Fetus_Hidup

Levene

Statistic df1 df2 Sig.

(6)

Ra nks

6 17.50

6 20.50

6 14.33

6 13.75

6 11.42

30 Perlak uan

K0 KP P1 P2 P3 Total Fetus_Hidup

N Mean Rank

Test Statistics

a,b

3.959

4

.412

Chi-Square

df

As ymp. Sig.

Fetus_Hidup

Kruskal Wallis Test

a.

Grouping Variable: Perlakuan

b.

Lampiran B.3. Uji Statistik Berat Badan Fetus Mencit Perlakuan Ekstrak

N-heksan Buah Andaliman

Tests of Normality

.271 6 .193 .849 6 .154

.159 6 .200* .937 6 .638

.211 6 .200* .962 6 .831

.256 6 .200* .780 6 .038

.290 6 .125 .816 6 .082

Perlakuan K0 KP P1 P2 P3 Berat_Badan_Fetus

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true s ignificance. *.

Lilliefors Significance Correction a.

Test of Homogeneity of Variance

.640 4 25 .639

.378 4 25 .822

.378 4 16.618 .821

.543 4 25 .705

Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Berat_Badan_Fetus

Levene

Statistic df1 df2 Sig.

ANOV A

Berat_Badan_Fetus

2.724 4 .681 3.434 .023

4.957 25 .198

7.680 29

Between Groups W ithin Groups Total

Sum of

Squares df Mean S quare F Sig.

(7)

Multiple Comparisons

Dependent Variable: Berat_Badan_Fetus

.00167 .25708 1.000 -.7897 .7930 -.04167 .25708 1.000 -.8330 .7497 .64167 .25708 .195 -.1497 1.4330 .55500 .25708 .407 -.2364 1.3464 -.00167 .25708 1.000 -.7930 .7897 -.04333 .25708 1.000 -.8347 .7480 .64000 .25708 .198 -.1514 1.4314 .55333 .25708 .412 -.2380 1.3447 .04167 .25708 1.000 -.7497 .8330 .04333 .25708 1.000 -.7480 .8347 .68333 .25708 .135 -.1080 1.4747 .59667 .25708 .287 -.1947 1.3880 -.64167 .25708 .195 -1.4330 .1497 -.64000 .25708 .198 -1.4314 .1514 -.68333 .25708 .135 -1.4747 .1080 -.08667 .25708 1.000 -.8780 .7047 -.55500 .25708 .407 -1.3464 .2364 -.55333 .25708 .412 -1.3447 .2380 -.59667 .25708 .287 -1.3880 .1947 .08667 .25708 1.000 -.7047 .8780 (J) Perlakuan

KP (I) Perlakuan K0

(I-J) Std. Error Sig. Lower Bound Upper Bound 95% Confidence Interval

Homogeneous Subsets

Berat_Badan_Fetus

6 .3967

6 .4833 .4833

6 1.0367 1.0367

6 1.0383 1.0383

6 1.0800

.739 .050 .875 Perlakuan Duncana

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed. Us es Harmonic Mean Sample Size = 6.000. a.

(8)

Te sts of Norm a litya,b ,c,d, e

Cl eft_P alate is constant when

Perlak uan = K 0. It has been om itted. a.

Cl eft_P alate is constant when

Perlak uan = K P. It has been om itted. b.

Cl eft_P alate is constant when

Perlak uan = P 1. It has been om itted. c.

Cl eft_P alate is constant when

Perlak uan = P 2. It has been om itted. d.

Cl eft_P alate is constant when

Perlak uan = P 3. It has been om itted.

Kruskal-Wallis Test

Ra nks Perlak uan

K0 Cleft_P alat e

N Mean Rank

Test Statistics

a,b

.000

Kruskal Wallis Test

a.

Grouping Variable: Perlakuan

b.

Lampiran C.2. Uji Statistik Kelainan Mikrophthalmia (%) Fetus Mencit

Perlakuan Ekstrak N-heksan Buah Andaliman

Te sts of Norm alityb

.492 6 .000 .496 6 .000

.406 6 .002 .678 6 .004

.229 3 . .981 3 .738

.219 3 . .987 3 .783

Perlak uan KP Kolmogorov-Sm irnova Shapiro-W ilk

Lilliefors Significanc e Correc tion a.

Mikrophthalmia is c ons tant when Perlak uan = K0. It has been om itted. b.

Te st o f Ho mo gen eity of V ari ancea

Mi krophthalmia is cons tant when Perlak uan = K 0. It has been om itted. a.

(9)

Ra nks

6 8.50

6 10.17

6 12.58

3 19.50

3 18.00

24 Perlak uan

K0 KP P1 P2 P3 Total Mikrophthalmia

N Mean Rank

Te st S tatisticsa,b

10.418 4 .034 Chi-Square

df

As ymp. Si g.

Mi krophth alm ia

Kruskal W allis Tes t a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

6

6.00

36.00

6

7.00

42.00

12

Perlakuan

K0

KP

Total

Mikrophthalmia

N

Mean Rank

Sum of Ranks

Te st S tatistics

b

15.000

36.000

-1. 000

.317

.699

a

Mann-W hit ney U

W ilcox on W

Z

As ymp. Sig. (2-tailed)

Ex act Sig. [2*(1-tailed

Sig.)]

Mikrophth

almia

Not correct ed for ties.

a.

Grouping V ariable: Perlakuan

b.

Mann-Whitney Test

Ranks

6

5.50

33.00

6

7.50

45.00

12

Perlakuan

K0

P1

Total

Mikrophthalmia

(10)

Te st S tatistics

b

Mann-W hit ney U

W ilcox on W

Z

As ymp. Si g. (2-tailed)

Ex act Sig. [2*(1-tai led

Sig.)]

Mi krophth

alm ia

Not correct ed for ties.

a.

Groupi ng V ariable: Perl akuan

b.

Mann-Whitney Test

Ranks

N

Mean Rank

Sum of Ranks

Te st S tatistics

b

.000

21.000

-2. 761

.006

.024

a

Mann-W hit ney U

W ilcox on W

Z

As ymp. Si g. (2-tailed)

Ex act Sig. [2*(1-tai led

Sig.)]

Mi krophth

alm ia

Not correct ed for ties.

a.

Groupi ng V ariable: Perl akuan

b.

Mann-Whitney Test

Ranks

N Mean Rank Sum of Ranks

Te st S tatisticsb

3.000 24.000 -2. 121 .034

.167a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Mi krophth alm ia

Not correct ed for ties. a.

(11)

Mann-Whitney Test

Ranks

6 5.83 35.00

6 7.17 43.00

12 Perlakuan

KP P1 Total Mikrophthalmia

N Mean Rank Sum of Ranks

Te st S tatistics

b

14.000

35.000

-.841

.400

.589

a

Mann-W hit ney U

W ilcox on W

Z

As ymp. Si g. (2-tailed)

Ex act Sig. [2*(1-tai led

Sig.)]

Mi krophth

alm ia

Not correct ed for ties.

a.

Groupi ng V ariable: Perl akuan

b.

Mann-Whitney Test

Ranks

6

3.75

22.50

3

7.50

22.50

9

Perlakuan

KP

P2

Total

Mikrophthalmia

N

Mean Rank

Sum of Ranks

Te st S tatisticsb

1.500 22.500 -2. 132 .033 .048a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Mi krophth alm ia

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

6

4.08

24.50

3

6.83

20.50

9

Perlakuan

KP

P3

Total

Mikrophthalmia

(12)

Te st S tatisticsb Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Mi krophth alm ia

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

N Mean Rank Sum of Ranks

Te st S tatistics

b

4.000

25.000

-1. 348

.178

.262

a

Mann-W hit ney U

W ilcox on W

Z

As ymp. Si g. (2-tailed)

Ex act Sig. [2*(1-tai led

Sig.)]

Mi krophth

alm ia

Not correct ed for ties.

a.

Groupi ng V ariable: Perl akuan

b.

Mann-Whitney Test

Ranks

N

Mean Rank

Sum of Ranks

Te st S ta tisticsb

Mi krop hth alm ia

No t co rrect ed for tie s. a.

Groupi ng V aria ble: Perl akua n b.

(13)

Ranks

3

3.33

10.00

3

3.67

11.00

6

Perlakuan

P2

P3

Total

Mikrophthalmia

N

Mean Rank

Sum of Ranks

Te st S tatistics

b

4.000

10.000

-.218

.827

1.000

a

Mann-W hit ney U

W ilcox on W

Z

As ymp. Si g. (2-tailed)

Ex act Sig. [2*(1-tai led

Sig.)]

Mi krophth

alm ia

Not correct ed for ties.

a.

Groupi ng V ariable: Perl akuan

b.

Lampiran C.3. Uji Statistik Kelainan Acorea (%) Fetus Mencit Perlakuan

Ekstrak N-heksan Buah Andaliman

Te sts of Norm alityb,c ,d

.385 3 . .750 3 .000

.385 3 . .750 3 .000

Perlak uan P2 P3 Ac orea

St atist ic df Sig. St atist ic df Sig. Kolmogorov-Smirnova Shapiro-W ilk

Lilliefors Significanc e Correc tion a.

Ac orea is c ons tant when Perlak uan = K0. It has been omitted. b.

Ac orea is c ons tant when Perlak uan = KP . It has been omitted. c.

Ac orea is c ons tant when Perlak uan = P1. It has been omitted. d.

Te st o f Ho mo ge n e ity of Va ri a nc ea ,b

Ac orea is cons tan t wh en P erla kua n = K0. It ha s be en omit ted.

a.

Ac orea is cons tan t wh en P erla kua n = KP. It ha s b een omi tted.

b.

Ac orea is cons tan t wh en P erla kua n = P1. It ha s be en omit ted.

c.

Kruskal-Wallis Test

Ra nks

6 11.50 6 11.50 6 11.50 3 15.67 3 15.33 24

Perlak uan K0 KP P1 P2 P3 Total Ac orea

(14)

Test Statisticsa,b

6.275 4 .180 Chi-Square

df

As ymp. Sig.

Acorea

Kruskal Wallis Test a.

Groupi ng Variable: Perlakuan b.

Lampiran C.4. Uji Statistik Kelainan Hidrocephalus (%) Fetus Mencit

Perlakuan Ekstrak N-heksan Buah Andaliman

Tests of Normality

.275 6 .175 .802 6 .061

.252 6 .200* .861 6 .194

.271 6 .191 .846 6 .147

.359 3 . .810 3 .138

.385 3 . .750 3 .000

Perlakuan K0 KP P1 P2 P3 Hidrocephalus

Statistic df Sig. Statistic df Sig. Kolmogorov-Smirnova Shapiro-Wilk

This is a lower bound of the true s ignificance. *.

Lilliefors Significance Correction a.

Kruskal-Wallis Test

Ra nks

6 7.33

6 7.67

6 18.42

3 13.83

3 19.33

24 Perlak uan

K0 KP P1 P2 P3 Total Hidroc ephalus

N Mean Rank

Te st S tatisticsa,b

13.254 4 .010 Chi-Square

df

As ymp. Si g.

Hidroc ep halus

Kruskal W allis Tes t a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

6 6.17 37.00 6 6.83 41.00 12

Perlakuan K0 KP Total Hidrocephalus

(15)

Te st S tatisticsb Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

N Mean Rank Sum of Ranks

Te st S tatisticsb

2.000 23.000 -2. 585 .010

.009a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

N Mean Rank Sum of Ranks

Te st S tatisticsb

4.000 25.000 -1. 313 .189 .262a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

(16)

Ranks

N Mean Rank Sum of Ranks

Te st S tatisticsb

1.000 22.000 -2. 110 .035

.048a Mann-W hit ney U

W ilcox on W Z

As ymp. Sig. (2-tailed) Ex act Sig. [2*(1-tailed Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Grouping V ariable: Perlakuan b.

Mann-Whitney Test

Ranks

N Mean Rank Sum of Ranks

Te st S tatisticsb

2.000 23.000 -2. 576 .010 .009a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

N

Mean Rank

Sum of Ranks

Te st S tatisticsb

2.000 23.000 -1. 823 .068

.095a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

(17)

Mann-Whitney Test

Ranks

6

3.67

22.00

3

7.67

23.00

9

Perlakuan

KP

P3

Total

Hidrocephalus

N

Mean Rank

Sum of Ranks

Te st S tatisticsb

1.000 22.000 -2. 092 .036 .048a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

6 5.75 34.50

3 3.50 10.50

9 Perlakuan

P1 P2 Total Hidrocephalus

N Mean Rank Sum of Ranks

Te st S tatisticsb

4.500 10.500 -1. 172 .241

.262a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

6 4.83 29.00

3 5.33 16.00

9 Perlakuan

P1 P3 Total Hidrocephalus

(18)

Te st S tatisticsb

8.000 29.000 -.260 .795

.905a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

Groupi ng V ariable: Perl akuan b.

Mann-Whitney Test

Ranks

3

2.33

7.00

3

4.67

14.00

6

Perlakuan

P2

P3

Total

Hidrocephalus

N

Mean Rank

Sum of Ranks

Te st S tatisticsb

1.000 7.000 -1. 550 .121 .200a Mann-W hit ney U

W ilcox on W Z

As ymp. Si g. (2-tailed) Ex act Sig. [2*(1-tai led Sig.)]

Hidroc ep halus

Not correct ed for ties. a.

(19)

Lampiran D. Pembuatan Ekstrak N-heksan Buah Andaliman

Dibersihkan

Dicuci

Dikeringkan

Dihaluskan

Ditimbang

Dimasukkan ke dalam botol cokelat

Dimaserasi ± 1 malam dalam pelarut N-heksan

Disaring

Dirotavapor

Dibuat dosis

Dimaserasi kembali dengan

Dilarutkan dalam CMC 1%

pelarut N-heksan

Disaring

Diambil 0,3 ml

Diberikan secara oral

dengan jarum

gavage

selama 10 hari

Buah Andaliman

Simplisia

Maserat

Filtrat

Residu

Ekstrak kental

Filtrat

Residu

Larutan ekstrak

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Sel hati (hepatosit) yang berbentuk polihedral, intinya bulat terletak di tengah, nukleolus dapat.. satu atau lebih dengan kromatin yang menyebar. Sitoplasma hepatosit agak