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LAMPIRAN
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LAMPIRAN 1 ROADMAP PENELITIAN
Sumber kolagenase
organisme perairan
Purifikasi dan karakterisasi Kolagenase
Kelenjar pencernaan Simpson (2000): Kepiting, lobster, udang Hepatopankreas
Aoki (2003) : Udang (Pandalus eolus)
Rudenskaya (2004) : Kepiting raja (Paralithodes camtschaticus) Organ dalam Kim (2002): filefish (Novodon modestrus) Park (2002): makerel (Scomber japanicus) Pilorik kaeka: Byun (2002) Tuna (Thunnus thynnus)
KOLAGENASE
Usus
Pilorik kaeka Hati Bandeng (Chanos chanos, forskal)
Jaringan Kim (1991): (Novodon modestrus)
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Lampiran 2
Tabel Konversi dari g (gravity) ke rpm (revolutions per minute) Konversi dari g ke rpm berdasarkan rumus:
Dimana: g = gravity atau gaya sentrifugal relatif (RCF = Relative Centrifugal Force)
R = jari-jari rotor (cm)
S = kecepatan putaran dalam rpm (revolutions per minute)
Sumber: Pierce Biotechnology, Inc. 2005. Technical Resource Convert Between Times Gravity (×G) And Centrifuge Rotor Speed (Rpm). Rockford, USA: Pierce Biotechnology, Inc. www.piercenet.com.
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Lampiran 4 Organ dalam ikan bandeng (Chanos chanos, Forskal)
Hati
Pilorik kaeka
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Lampiran 5 Hasil perhitungan elekroforesis No Marker Batas bawah (cm) Batas atas (cm) BM Rf Log BM 1 Phosphorilase b 5 0,7 97.000 0,14 4,986772 2 Albumin 5 1,5 66.000 0,3 4,819544 3 Oval bumin 5 2,3 45.000 0,46 4,653213 4 Carbonic anhidrase 5 3,1 30.000 0,62 4,477121 5 tripsin inhibitor 5 3,4 20.100 0,68 4,303196 6 α-laktalbumin 5 4,0 14.400 0,80 4,158362 7 Band 1 5 3,0 27.612,14 0,60 4,44110 8 Band 2 5 4,1 14.636,59 0,82 4,16544
Kurva regresi linear berat molekul marker dan Rf
y = -1,253x + 5,1929 R2 = 0,9828 0 1 2 3 4 5 6 0 0,2 0,4 0,6 0,8 1 Rf Lo g BM
