تاﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ يردﻮﭘ هرﺎﺼﻋ
ﻞﻣﺎﻛ هﻮﻴﻣ و
بآ هﻮﻴﻣ و رﺎﻧا ﻲﻧﺎﻴﻠﻗ وﺪﻛ
)
Bottle Gourd
( يور ﺮﺑ يﺮﺘﻛﺎﺑ
ﻲﻔﻨﻣ مﺮﮔ و ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ
ترﺪﻗ ﺢﺻﺎﻧ ﻪﻟا ﻦﺴﺣ ﺪﻤﺤﻣ ،1
دﺮﻓ رﻮﭘ رﺎﻜﻧادﻮﺑ شﺎﻬﺑﻮﺳ ،2
رﻮﻫدﺎﻣ ،3
ﻳ ﺎﻤ ﺖﻴﺸﻜﻳد 4
هﺪﻴﻜﭼ
ﻪﻨﻴﻣز فﺪﻫ و يﺎﻫوراد زا هدﺎﻔﺘﺳا : ﻲﺘﻧآ
ﻜﻴﺗﻮﻴﺑ ﻲ ﺮﻴﻈﻧ ﻲﺗﻼﻜﺸﻣ ﺎﺑ ﺖﻣوﺎﻘﻣ و ﻪﺘﺳاﻮﺧﺎﻧ ﻲﺒﻧﺎﺟ ضراﻮﻋ
ﻲﻳوراد ﺖﺳا هاﺮﻤﻫ .
نﺎﻫﺎﻴﮔ
ﻲﻣ ﺪﻧﻮﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﺐﺳﺎﻨﻣ ﻦﻴﺸﻧﺎﺟ ﻚﻳ ناﻮﻨﻋ ﻪﺑ ﺪﻨﻧاﻮﺗ
؛ ﺪﻨﺷﺎﺑ يﺮﺘﻤﻛ ﻲﺒﻧﺎﺟ ضراﻮﻋ ياراد ﺖﺳا ﻦﻜﻤﻣ اﺮﻳز .
و رﺎﻧا ﺎﻴﻠﻗ وﺪﻛ ﻧ ﻲ
)
Bottle Gourd
( هﻮﻴﻣ زا ﺎﻫ يرﺎﻴﺴﺑ رد ﺖﻟﻮﻬﺳ ﻪﺑ ﻪﻛ ﺪﻨﺘﺴﻫ ﻲﻳ زا
ﺖﻓﺎﻳ ﻖﻃﺎﻨﻣ ﻲﻣ
ﺪﻧﻮﺷ . ﻌﺗ فﺪﻫ ﺎﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﻴﻴ
ﻦ ﺮﺛا
ﺪﺿ
ﺑ ﻲﻳﺎﻳﺮﺘﻛﺎ ﺷ مﺎﺠﻧا ﺎﻬﻧآ ﺪ
.
شور ﻖﻴﻘﺤﺗ ا رد: ﻳ ﻦ ﺑﺮﺠﺗ ﻪﻌﻟﺎﻄﻣ ﻲ
و رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ يردﻮﭘ هرﺎﺼﻋ ﻲﻧﺎﻴﻠﻗ وﺪﻛ
بآ و ﺎﻬﻧآ ﺖﺳﻮﭘ نوﺪﺑ هﻮﻴﻣ رﻮﻈﻨﻣ ﻪﺑ
ﻲﺳرﺮﺑ تاﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ و ﻪﻴﻬﺗ ﺶﺷ ﻪﻴﻠﻋ ﺮﺑ ﺖﻴﻠﭘ ﻚﺴﻳد شور زا هدﺎﻔﺘﺳا ﺎﺑ ﮔ
ﻧﻮ ﺔ ﻞﻣﺎﺷ ،يﺮﺘﻛﺎﺑ ﻒﻠﺘﺨﻣ ﻲﻔﻴﺗ ﻼﻧﻮﻤﻟﺎﺳ
ﺸﻳﺮﺷا ، ﺎﻴ ﻲﻠﻛ ﻼﻴﺴﺒﻠﻛ ،
ﻪﻴﻧﻮﻣﻮﻨﭘ سﻮﺋﺮﺳ سﻮﻠﻴﺳﺎﺑ ، ﺲﻴﻠﻴﺘﺑﻮﺳ سﻮﻠﻴﺳﺎﺑ ،
يﺮﺘﻧﺎﺴﻳد ﻼﮕﻴﺷ و ﺪﺷ مﺎﺠﻧا
. ﻪﻧﻮﻤﻧ ندوﺰﻓا ﺎﺑ ﺎﻫ
ﻦﻴﻠﻜﻴﺳاﺮﺘﺗ و )
ﺑ ﻪ ﻊﺟﺮﻣ ناﻮﻨﻋ (
رد
يﺮﺘﻛﺎﺑ يوﺎﺣ ﺖﺸﻛ ﻂﻴﺤﻣ
، هزاﺪﻧا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ناﺰﻴﻣ ﺪﺷ يﺮﻴﮔ
) ﻞﻗاﺪﺣ 3 ﺮﻫ ياﺮﺑ رﺎﺑ ﻪﻧﻮﻤﻧ
(.
ياﺮﺑ هوﺮﮔ ﻦﻴﺑ ﻪﺴﻳﺎﻘﻣ زا ﺎﻫ
نﻮﻣزآ ﺎﻫ و ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ ي ﻲﻛﻮﺗ
ﻲﻨﻌﻣ ﺢﻄﺳ رد يراد
05 /
P<0 ﺪﻳدﺮﮔ هدﺎﻔﺘﺳا .
ﻪﺘﻓﺎﻳ ﺎﻫ هرﺎﺼﻋ : يردﻮﭘ راﺪﻘﻣ ﺎﺑ رﺎﻧا 250
ﻣ ﻴ مﺮﮔوﺮﻜ د رد ﻳ ﻚﺴ ﺖﺴﻧاﻮﺗ ﻮﻠﺟ ي ﺮﺘﻛﺎﺑ ﺪﺷر ي ﺎﻫ ي ﻪﻌﻟﺎﻄﻣ درﻮﻣ ﺰﺟ ﻪﺑ
سﻮﻠﻴﺳﺎﺑ
ﺲﻴﻠﻴﺘﺑﻮﺳ ﺑ ار ﻪ ﻔﺧ رﻮﻃ ﻴ ﻒ ﮕﺑ ﻴ دﺮ
، بآ ﻣ ﻴ هﻮ ا رﺎﻧ و ﻲﻧﺎﻴﻠﻗ وﺪﻛ هرﺎﺼﻋ و
يردﻮﭘ ﻲﻧﺎﻴﻠﻗ وﺪﻛ ﻟﺎﻌﻓ
ﻴ ﺖ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﻧداﺪﻧ نﺎﺸﻧ .
ﻪﺠﻴﺘﻧ يﺮﻴﮔ
ﺪﺿ ﺖﻴﻟﺎﻌﻓ ياراد رﺎﻧا ﻞﻣﺎﻛ يردﻮﭘ هرﺎﺼﻋ ﻪﻛ داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ ﻦﻳا: ﻲﻔﻴﻔﺧ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ
ﻲﻣ ﻪﻛ ﺖﺳا ﺮﻳدﺎﻘﻣ زا ﻲﺷﺎﻧ ﺪﻧاﻮﺗ
ﻲﻠﭘ ياﺰﺟا ﺮﺘﺸﻴﺑ ﺪﺷﺎﺑ ﻲﻧﺎﻴﻠﻗ وﺪﻛ ﺎﺑ ﻪﺴﻳﺎﻘﻣ رد نآ ﻚﻴﻟﻮﻨﻓ
.
ژاو ه يﺪﻴﻠﻛ يﺎﻫ ،رﺎﻧا:
ﻛ ﺪ ﻲﻧﺎﻴﻠﻗ و ، ﺖﻴﻟﺎﻌﻓ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻦﻴﻠﻜﻴﺳاﺮﺘﺗ ،
ﺪﻨﺟﺮﻴﺑ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲﻤﻠﻋ ﻪﻠﺠﻣ .
1389
؛ 17 ) 4 :(
257 - 264
ﺖﻓﺎﻳرد : 18 / 03 / 1389 حﻼﺻا ﺎﻬﻧ ﻲﻳ : 15 / 12 / 1389 شﺮﻳﺬﭘ : 16 / 12 / 1389
لوﺆﺴﻣ هﺪﻨﺴﻳﻮﻧ1
، ﺣاﺮﺟ هوﺮﮔ رﺎﻳدﺎﺘﺳا ﻲ
، ﺑﻴ نﺎﺘﺳرﺎﻤ ﺎﺿر مﺎﻣا
) ع
،(
ﻜﺷﺰﭘ هﺪﻜﺸﻧاد ﻲ
، ﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲ
ﺪﻨﺟﺮﻴﺑ ناﺮﻳا ،
سردآ : ﺑﻴ ﺪﻨﺟﺮ - ﺧ ﻴ نﺎﺑﺎ ﻧﺎﻘﻟﺎﻃ ﻲ - ﺑﻴ ﻤ نﺎﺘﺳرﺎ ﺎﺿر مﺎﻣا
) ع
-(
ﺣاﺮﺟ ﺶﺨﺑ ﻲ
ﻦﻔﻠﺗ : 2222300 - 0561 ﺮﺑﺎﻤﻧ : 4430076 - 0561 ﻲﻜﻴﻧوﺮﺘﻜﻟا ﺖﺴﭘ [email protected]:
يژﻮﻟﻮﻛﺎﻣرﺎﻓ هوﺮﮔ رﺎﻳدﺎﺘﺳا2
، ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ،
ﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻲ
ﺪﻨﺟﺮﻴﺑ ناﺮﻳا ،
يژﻮﻟﻮﻛﺎﻣرﺎﻓ هوﺮﮔ دﺎﺘﺳا3
، زﺎﺳوراد هﺪﻜﺸﻧاد
،ي ﻲﺗارﺎﻬﺑ هﺎﮕﺸﻧاد
، ﺪﻨﻫ
ﻲﻤﻴﺷﻮﻴﺑ هوﺮﮔ دﺎﺘﺳا4
، ﺎﻧﻮﭘ هﺎﮕﺸﻧاد
، ﺪﻨﻫ
ﻪﻣﺪﻘﻣ ﻲﻳوراد ﻊﻳﺎﻨﺻ ﻪﺘﺷﺬﮔ ﻪﻫد ﻪﺳ رد ﺪﻨﭼﺮﻫ داﺪﻌﺗ
ﻞﺑﺎﻗ
ﻪﻈﺣﻼﻣ يا ا ز ﻲﺘﻧآ ﻚﻴﺗﻮﻴﺑ هدﺮﻛ ﺪﻴﻟﻮﺗ ار ﺎﻫ ﺖﻣوﺎﻘﻣ ﺎﻣا ﺪﻧا
ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺎﻫ
ﺖﺒﺴﻧ ﺖﺳا ﻪﺘﻓﺎﻳ ﺶﻳاﺰﻓا ﺎﻫوراد ﻦﻳا ﻪﺑ
؛
ﺑ ﻪ
ّﻠﻛ رﻮﻃ يﺮﺘﻛﺎﺑ ﻲ ﺎﻫ
ﺮﻈﻧ زا ﻲﺗاذ و ﻲﺑﺎﺴﺘﻛا ﺖﻣوﺎﻘﻣ دﺎﺠﻳا
ﺑ ﻪﻛ ﻲﻳﺎﻫوراد ﻪﺑ ﺖﺒﺴﻧ ﻪ
ﺑ ﻲﻧﺎﻣرد ﻞﻣاﻮﻋ ناﻮﻨﻋ ﻪ
رﺎﻛ
ﻲﻣ ﺪﻧور
، ﻲﻳﺎﻧاﻮﺗ ژ ﻲﻜﻴﺘﻧ ﺪﻧراد ) 1 ( ﻦﻳاﺮﺑﺎﻨﺑ ؛ ﺘﺴﻳﺎﺑ
ﻲ ﻲﺗﺎﻣاﺪﻗا
رﻮﻈﻨﻣ ﻪﺑ ﺶﻫﺎﻛ
ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺖﻣوﺎﻘﻣ زا ﻲﻜﻳ ﻪﻛ دﺮﻴﮔ مﺎﺠﻧا
هار ﺎﻫ
، و ﻲﻌﻴﺒﻃ ﻊﺑﺎﻨﻣ زا ﺪﻳﺪﺟ يﺎﻫوراد ﻒﺸﻛ ﻲﻋﺎﻨﺻ
ﻲﻣ ﺪﺷﺎﺑ
) 2 (.
1رﺎﻧا ﻪﭽﺘﺧرد هداﻮﻧﺎﺧ زا ﺖﺳا يا
ﻪﺳﺎﻜﻴﻧﻮﭘ
ناﺮﻳا ﻲﻣﻮﺑ ﻪﻛ
ﺪﻨﻫ و ) ﻲﻟﺎﻤﺷ ﺖﻤﺴﻗ (
ﻲﻣ و ﺪﺷﺎﺑ نﺎﻣز زا ﺮﺳاﺮﺳ رد ﻢﻳﺪﻗ يﺎﻫ
ﻪﻧاﺮﺘﻳﺪﻣ ﻪﻴﺣﺎﻧ ﺎﻴﺳآ يا
، ﺖﺸﻛ ﺎﭘورا و ﺎﻘﻳﺮﻓا ﻲﻣ
ﺖﺳا هﺪﺷ )
3 (.
ﻲﻛارﻮﺧ ﺖﻤﺴﻗ نآ
ﺑ ﻪ ترﻮﺻ ﻪﺑ ﻦﻴﻨﭽﻤﻫ و هزﺎﺗ ترﻮﺻ
ﺮﻓ ا هدرو ﺎﻫ بآ ﻞﻴﺒﻗ زا ﻲﻳ هﻮﻴﻣ
، ﺎﺑﺮﻣ ،بر ﺑ ﻲﻧدوﺰﻓا ﺎﻳ و
ﻪ ناﻮﻨﻋ
ﻢﻌﻃ ﮓﻧر و هﺪﻨﻫد ﻲﻧﺪﻴﺷﻮﻧ ﺮﮕﻳد رد هﺪﻨﻫد
ﺎﻫ هدﺎﻔﺘﺳا درﻮﻣ
راﺮﻗ ﻲﻣ دﺮﻴﮔ ) 4 .(
رﺎﻧا هﻮﻴﻣ زا ﻲﻨﻏ ﻲﻳﺎﻴﻤﻴﺷ تﺎﺒﻴﻛﺮﺗ ﺮﻈﻧ زا
ﻼﻓ ﺎﻫﺪﻴﺋﻮﻧو )
0 / 1 - 2 / 0 (%
ﻲﻣ ﺪﺷﺎﺑ . ﺒﻳﺮﻘﺗ
ًﺎ 30 % ﻤﻫ زا ﻪ
ﻦﻳﺪﻴﻧﺎﻴﺳﻮﺘﻧآ ﺎﻫ
2ي نآ ﺖﺳﻮﭘ رد رﺎﻧا رد دﻮﺟﻮﻣ ﺖﺳا
؛ ﻦﻴﻨﭽﻤﻫ
يوﺎﺣ رﺎﻧا هﻮﻴﻣ 4
/ 85 %
،ﺖﺑﻮﻃر 6
/ 10 % ،ﺪﻨﻗ 4 / 1 % و ﻦﻴﺘﻜﭘ
1 / 0 ﻚﻳﺮﺘﻴﺳ ﺪﻴﺳا مﺮﮔ )
ﻪﻧﻮﮔ ﺮﺘﺸﻴﺑ رد ﻲﻠﺻا ﺪﻴﺳا ﺎﻫ
نآ ي ( و
ﺮﮕﻳد داﻮﻣ ﻲﻣ
ﺪﺷﺎﺑ ) 6،5 (.
ﺗﺎﺒﻴﻛﺮﺗ رﺎﻧا ﻪﻧاد زا ﺪﻨﻧﺎﻣ ﻲ
نوﻼﻓوﺰﻳا
،3
ﻦﻴﺌﺘﺴﻴﻨﺟ ﻦﻴﺘﺴﻴﻨﺟ ،4
ﻦﻴﺋزدﺎﻳد ،5
ﻦﻳزدﺎﻳد ،6
و 7
وﺮﺘﺳا ل جاﺮﺨﺘﺳا ﺖﺳا هﺪﺷ
) 8،7 ( . ﻦﻳﮋﻟﺎﻜﻴﻧﻮﭘ ﺴﻣ 8
ﺆ لو
ﻲﺘﻧآ ﺖﻴﻟﺎﻌﻓ رﺎﻧا يﻻﺎﺑ ﻲﻧاﺪﻴﺴﻛا
ﻲﻣ ﺪﺷﺎﺑ . ﻲﺧﺮﺑ ياراد رﺎﻧا
ﻲﺘﻧآ تﺎﺒﻴﻛﺮﺗ زا ﺮﮕﻳد ﻲﻧاﺪﻴﺴﻛا
ﻲﻣ ﺪﺷﺎﺑ ) 10،9 ( .
ﺐﻃ رد ﻲﺘﻨﺳ
نﺎﻧﻮﻳ زا رﺎﻧا بآ ياﺮﺑ
ﻟا نﺎﻣرد مﺮﻛ ،بﺎﻬﺘ
هدور يﺎﻫ ،يا
ﻪﻓﺮﺳ ﺎﻫ لﺎﻬﺳا و مواﺪﻣ ي ﻲﻣ هدﺎﻔﺘﺳا
هﺪﺷ ﺖﺳا
؛ ﻦﻴﻨﭽﻤﻫ
Punica granatum L. or Pomegranate١
٢
Anthocyanidin
٣
Isoflovan
٤
Genistein
Genistin٥ Diadzein٦
Diadzin٧ Punicalagins٨
نآ فﺮﺼﻣ رﻮﻈﻨﻣ ﻪﺑ
زا يﺮﻴﮕﺸﻴﭘ و ﻲﻗوﺮﻋ ﻢﺘﺴﻴﺳ ﺖﻳﻮﻘﺗ
ﻦﻴﻳاﺮﺷ ﺐﻠﺼﺗ ﺰﻳﻮﺠﺗ ﺖﻴﺸﻧوﺮﺑ و ﻢﺳآ ،
ﻲﻣ ﺖﺳا هﺪﺷ )
11 .(
ﺮﻴﺧا
ًا
ﺪﺿ تاﺮﺛا ﺖﻴﻟﺎﻌﻓ رﺎﻬﻣ ﺎﺑ ﻪﻛ رﺎﻧا هﻮﻴﻣ هرﺎﺼﻋ ﻲﺑﺎﻬﺘﻟا
ﻢﻳﺰﻧآ ﺎﻫ ﺪﻴﻟﻮﺗ ي زﻮﻜﻳا هﺪﻨﻨﻛ ﺪﻴﺋﻮﻧا
ﺖﺳا هاﺮﻤﻫ 9
، هﺪﺷ شراﺰﮔ
ﺖﺳا ) 12 ( . ﻲﻧﺎﻴﻠﻗ وﺪﻛ هداﻮﻧﺎﺧ زا 10
ﻪﺳﺎﺘﻴﺑرﻮﻛﻮﻛ ﻲﻣ 11
ﺪﺷﺎﺑ . هﺎﻴﮔ ﻦﻳا
ﺑ ﺪﻨﻫ ﺮﺳاﺮﺳ رد ﻪ
ﺖﺸﻛ و ﻲﺸﺣو ترﻮﺻ و دراد دﻮﺟو هﺪﺷ
ﺑ ﻪ
راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ لﺎﺳ لﻮﻃ مﺎﻤﺗ رد يﺰﺒﺳ ناﻮﻨﻋ ﻲﻣ
دﺮﻴﮔ .
هﻮﻴﻣ رد ﻲﻧﺎﻴﻠﻗ وﺪﻛ
ﺮﻴﻈﻧ ﻲﺗﺎﺒﻴﻛﺮﺗ لوﺮﺘﺳا ،ﺪﻴﺋﻮﻧوﻼﻓ
،
ﻧﻮﭘﺎﺳ
12ﻦﻴ ﻦﻴﺳﺎﺘﻴﺑرﻮﻛﻮﻛ ، ﻲﻠﭘ تﺎﺒﻴﻛﺮﺗ ،13
و ﻦﻴﺌﺗوﺮﭘ ،ﻚﻴﻟﻮﻨﻓ
دراد دﻮﺟو تارﺪﻴﺋﻮﺑﺮﻛ )
14،13 .(
ﺐﻃ رد ﻲﺘﻨﺳ
زا ﻪﻤﻫ
ﺖﻤﺴﻗ يﺎﻫ ﻲﻧﺎﻴﻠﻗ وﺪﻛ ﻞﮔ و ﻪﻧاد ،ﻪﺸﻳر ،گﺮﺑ ،هﻮﻴﻣ ﻞﻣﺎﺷ
ﻲﻧﺎﻣرد هدﺎﻔﺘﺳا ﻲﻣ
دﻮﺷ .
رﺪﻣ تاﺮﺛا ياراد هﻮﻴﻣ
ﺪﺿ ، ،اﺮﻔﺻ
يﺮﻴﮔﻮﻠﺟ
ﺪﺿ ،ﮓﻨﺳ ﻞﻴﻜﺸﺗ زا هﺪﻨﻨﻛ ﻲﻗ ،ﻪﻓﺮﺳ
ﻣ و روآ ﻞﻬﺴ
ﻲﻣ ﺑ ﻦﻴﻨﭽﻤﻫ و ﺪﺷﺎﺑ ﻪ
ﻲﻃ رد اﺬﻏ ناﻮﻨﻋ درﻮﻣ لﺎﺳ ناراﺰﻫ
راﺮﻗ هدﺎﻔﺘﺳا ﻲﻣ
ﺖﺳا ﻪﺘﻓﺮﮔ )
15 - 17 ( .
هرﺎﺼﻋ فﺮﺼﻣ ﺎﻫ
و ﻰﻣﺮﻓوﺮﻠﻛ ى رد ﻰﻠﻜﻟا
ﻲﻳاﺮﺤﺻ شﻮﻣ
ﻰﻨﻌﻣ ﺶﻫﺎﻛ ﺚﻋﺎﺑ ﺮﺗ ،مﺎﺗ لوﺮﺘﺴﻠﻛ راﺪﻘﻣ رد ىراد
ﻳ
،ﺪﻳﺮﻴﺴﻴﻠﮕ
14LDL ﺶﻳاﺰﻓا و
15HDL ﺖﺳا هﺪﺷ .
،يﺪﺒﻛ ﺖﻇﺎﻔﺣ تاﺮﺛا
ﺪﺿ ﻴﻈﻨﺗ و ﺖﺑﺎﻳد هﺪﺷ شراﺰﮔ هﻮﻴﻣ هرﺎﺼﻋ ﻲﻨﻤﻳا ﻢﺘﺴﻴﺳ ﻢ
ﺖﺳا ) 18،13 ( .
ﻦﻳا رد ﻪﻌﻟﺎﻄﻣ
، تاﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ يردﻮﭘ هرﺎﺼﻋ
و
بآ و رﺎﻧا هﻮﻴﻣ ﻲﻧﺎﻴﻠﻗ وﺪﻛ
زا ﻲﻳﻻﺎﺑ ﺮﻳدﺎﻘﻣ ياراد ﻪﻛ
ﻲﺘﻧآ هدﻮﺑ ناﺪﻴﺴﻛا
، ﻦﻴﻨﭽﻤﻫ ﻪﻠﻤﺟ زا
ﻪﺑ سﺮﺘﺳد رد نﺎﻫﺎﻴﮔ
رﺎﻤﺷ ﻲﻣ ﻧور ﺪ
، ﺶﺷ ﻪﻴﻠﻋ ﺮﺑ ﻒﻠﺘﺨﻣ ﻪﻧﻮﮔ
يﺮﺘﻛﺎﺑ
، ﻞﻣﺎﺷ
ﻟﺎﺳ ﻲﻔﻴﺗ ﻼﻧﻮﻤ
،16
ﻲﻠﻛ ﺎﻴﺸﻳﺮﺷا
،17
ﻼﻴﺴﺒﻠﻛ ﻮﻨﭘ
ﻪﻴﻧﻮﻣ
،18
Eicosanoid٩
١٠
Lagenaria siceraria Molina Standley
١١
Cucurbitaceae
١٢
Saponin
١٣
Cucurbitacin
١٤
Low Density Lipoprotein High Density Lipoprotein١٥
١٦
Salmonella typhi Escherichia coli١٧
Klebsiella pneumonia١٨
ﺮﺳ سﻮﻠﻴﺳﺎﺑ ﺋ
1
سﻮ ﺲﻴﻠﻴﺘﺑﻮﺳ سﻮﻠﻴﺳﺎﺑ
، و 2ﺮﺘﻧﺎﺴﻳد ﻼﮕﻴﺷ
3
ي
ﺖﻓﺮﮔ مﺎﺠﻧا .
زا ﺲﭘ ﻲﻠﺒﻗ نﻮﺘﻣ ﻲﺳرﺮﺑ ﺎﺗ
ﭻﻴﻫ ،ﻖﻴﻘﺤﺗ مﺎﺠﻧا نﺎﻣز
ﻲﺸﻫوﮋﭘ و ﻪﻟﺎﻘﻣ ﻪﻨﻴﻣز رد
تاﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ هرﺎﺼﻋ
ﺎﻫ ي
و رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ ﻲﻧﺎﻴﻠﻗ وﺪﻛ
ﺪﻣﺎﻴﻧ ﺖﺳد ﻪﺑ .
ر شو ﻖﻴﻘﺤﺗ
1 - هرﺎﺼﻋ جاﺮﺨﺘﺳا و نﺎﻫﺎﻴﮔ ﺎﻫ
:
ﻲﺑﺮﺠﺗ ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد
، هﻮﻴﻣ و رﺎﻧا ﻲﻧﺎﻴﻠﻗ وﺪﻛ ياﺮﺑ
بﺎﺨﺘﻧا ﻪﻌﻟﺎﻄﻣ ﺷ
ﺪﻧﺪ و رازﺎﺑ زا ﺎﻬﻧآ هﻮﻴﻣ ﺎﻧﻮﭘ ﺮﻬﺷ
و يراﺪﻳﺮﺧ
هﺎﻴﮔ ﻂﺳﻮﺗ سﺎﻨﺷ
Dr. A.M. Mujumdar ) (
ﻪﺴﺳﺆﻣ رد
ﻲﺗﺎﻘﻴﻘﺤﺗ Agharkar
ﻲﻳﺎﺳﺎﻨﺷ ﺪﻴﻳﺄﺗ و
ﺪﻳدﺮﮔ
؛ ﺲﭙﺳ ﺮﻫ
هﻮﻴﻣ زا ماﺪﻛ ﺎﻫ
ﺑ ﻪﻧﺎﮔاﺪﺟ ﻪ
ﻞﻣﺎﻛ رﻮﻃ )
ﻪﻧاد و ﺖﺳﻮﭘ هاﺮﻤﻫ ﻪﺑ
ﭗﻟﺎﭘ و ( رﻮﻈﻨﻣ ﻪﺑ هرﺎﺼﻋ
دﺮﺧ بﺎﻴﺳآ ﺎﺑ يﺮﻴﮔ ﺷ
ﺪﻧﺪ .
هرﺎﺼﻋ يﺮﻴﮔ مﺎﻤﺗا زا ﺪﻌﺑ و ﺖﻓﺮﮔ ترﻮﺻ بآ لﻼﺣ ﺎﺑ
تﺎﻴﻠﻤﻋ
، هرﺎﺼﻋ ﺎﻫ زا هدﺎﻔﺘﺳا ﺎﺑ هﺎﮕﺘﺳد
ﺮﻴﻄﻘﺗ ﻸﺧ رد ﻪﺟرد رد
دوﺪﺣ ﻲﺗراﺮﺣ 50
ﺪﻧﺪﺷ ﻆﻴﻠﻐﺗ ،داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد .
ﺎﻬﺘﻧا رد
دﻮﭘ هرﺎﺼﻋ ﺪﻳدﺮﮔ ﻪﻴﻬﺗ ماﺪﻛ ﺮﻫ ير
. بآ هﻮﻴﻣ ﺎﻫ رﺎﻧا ي ) نوﺪﺑ
ﺖﺳﻮﭘ ( و ﻲﻧﺎﻴﻠﻗ وﺪﻛ ﺑ
ﻪ ﻪﻧﺎﮔاﺪﺟ رﻮﻃ ﻪﻴﻬﺗ
ﺪﻧﺪﺷ .
2 -
ﺪﺿ تاﺮﺛا ﻲﺳرﺮﺑ ﻲﺑوﺮﻜﻴﻣ
:
يﺮﺘﻛﺎﺑ
ًﻼﺒﻗ ﻪﻛ ﺎﻫ ﺗ ﻪﺑ
ﺎ هﺎﮕﺸﻳﺎﻣزآ ﺪﻴﻳ ﻊﺟﺮﻣ
ﺪﻧدﻮﺑ هﺪﻴﺳر
،
ﺎﻧﻮﭘ هﺎﮕﺸﻧاد يژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ ﺶﺨﺑ زا )
نﺎﺘﺳوﺪﻨﻫ (
ﻪﻴﻬﺗ ﺪﻧﺪﺷ و
ﺘﻓﺮﮔ راﺮﻗ ﺶﻳﺎﻣزآ درﻮﻣ ﺪﻨ
. رد مﺎﻤﺗ نﻮﻣزآ ﺎﻫ يژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ ي
ﻂﻴﺤﻣ ﻪﺑ زﺎﻴﻧ ﻪﻛ
ﺪﺿ ﻲﻧﻮﻔﻋ زا هدﻮﺑ ﻮﻠﻓ رﺎﻨﻴﻣﻻ دﻮﻫ هدﺎﻔﺘﺳا
ﺪﺷ . ﻒﻟا - ﻂﻴﺤﻣ ﺎﻫ ﺖﺸﻛ ي :
ﻚﺴﻳد تﺎﺸﻳﺎﻣزآ رد ﺖﻴﻠﭘ
هدﺎﻔﺘﺳا رﺎﮔآ ﺖﺸﻛ ﻂﻴﺤﻣ زا
ﺪﺷ . مﺎﻤﺗ ﻂﻴﺤﻣ ﺎﻫ هﺪﻧزﺎﺳ ﻪﻧﺎﺧرﺎﻛ رﻮﺘﺳد سﺎﺳا ﺮﺑ ﺖﺸﻛ ي
ﻞﻳﺮﺘﺳا وﻼﻛﻮﺗا هﺎﮕﺘﺳد زا هدﺎﻔﺘﺳا ﺎﺑ و ﻪﻴﻬﺗ ﺷ
ﺪ ﻧ ﺪ . ﻂﻴﺤﻣ
ثاﺮﺑ ﺖﻨﻳﺮﺗﻮﻧ عﻮﻧ زا ﺖﺸﻛ دﻮﺑ4
.
Bacillos cereus١
٢
Bacillos subtilis
Shigella dysenteriae٣ Nutrient broth٤
ب - نﻮﻴﺳاﺰﻴﻠﻳﺮﺘﺳا :
ﺮﻳﺎﻣ ﻦﻟرا فﺮﻇ ﻚﻳ رد ثاﺮﺑ ﺖﻨﻳﺮﺗﻮﻧ ﻂﻴﺤﻣ
ﺖﻈﻠﻏ ﺎﺑ
رد ﺐﺳﺎﻨﻣ 100
ﻲﻠﻴﻣ ﺑ و ﻞﺣ ﺮﻄﻘﻣ بآ ﺮﺘﻴﻟ ﻪ
ﻞﻣﺎﻛ رﻮﻃ
رﺎﮔآ نآ ﻪﺑ و ﺪﻳدﺮﮔ طﻮﻠﺨﻣ 5
/ 2
% و ﻪﻓﺎﺿا 2 /
±0 9 / pH=6 رد
25 ﻪﺟرد ﺪﻳدﺮﮔ ﻢﻴﻈﻨﺗ داﺮﮕﻴﺘﻧﺎﺳ .
ﺑ لﻮﻠﺤﻣ ﻪ هﺪﻣآ ﺖﺳد ﻪﺑ
تﺪﻣ 20 تراﺮﺣ ﻪﺟرد رد ﻪﻘﻴﻗد 121
رد و داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد
رﺎﺸﻓ 15 لﺎﻜﺳﺎﭘ ﺪﻳدﺮﮔ وﻼﻛﻮﺗا .
زا هﺪﺷوﻼﻛﻮﺗا لﻮﻠﺤﻣ
، ﻪﺑ
راﺪﻘﻣ 15 ﻲﻠﻴﻣ ﺮﺘﻴﻟ رد زا ﻚﻳ ﺮﻫ ﺖﻴﻠﭘ
ﺎﻫ يﺮﺘﭘ ﻞﻳﺮﺘﺳا ي
نﺪﺷﺪﻤﺠﻨﻣ زا ﺲﭘ و ﻪﺘﺨﻳر ياﺮﺑ
تﺎﺸﻳﺎﻣزآ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ
ﺪﺷ هدﺮﺑ رﺎﻜﺑ .
ج ﻲﺳرﺮﺑ و ﺶﻳﺎﻣزآ شور– :
تاﺮﺛا
ﺪﺿ ﺑ ﻲﻳﺎﻳﺮﺘﻛﺎ ﻪﻴﻬﺗ ﻪﻧﻮﻤﻧ رﺎﻬﭼ
هﺪﺷ
، ﻞﻣﺎﺷ : بآ هﻮﻴﻣ
بآ ،رﺎﻧا هﻮﻴﻣ ﻲﻧﺎﻴﻠﻗ وﺪﻛ هرﺎﺼﻋ و
ﺎﻫ ي يردﻮﭘ ﻲﻧﺎﻴﻠﻗ وﺪﻛ و
ﺎﺑ رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ μl ﺖﻈﻠﻏ
mg/100 5 ﺎﺑ ﺖﻴﻠﭘ ﻚﺴﻳد شور
ﻪﻴﻠﻋ ﺮﺑ ﺶﺷ ﻪﻧﻮﮔ يﺮﺘﻛﺎﺑ رﻮﻛﺬﻣ ﺪﻳدﺮﮔ ﻦﻴﻴﻌﺗ )
20،19 (.
ﻞﺣ ﺎﺑ شور ﻦﻳا ﻖﺒﻃ هرﺎﺼﻋ ندﺮﻛ
ﺎﻫ رد يردﻮﭘ ي
يد ﻞﻴﺘﻣ ﻣﺮﻓ ﺎ ﻣ
5ﺪﻴ μl ﺖﻈﻠﻏ mg/100
5 ﻪﻴﻬﺗ ﺪﻳدﺮﮔ
؛ ﺲﭙﺳ
ﺖﺸﻛ ﺎﻫ ﺎﺑ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺶﺷ ي ﻢﺠﺣ
ﺺﺨﺸﻣ يﺮﺘﻛﺎﺑ
CFU/ml) 105
2 ml, / 0 ( ﺖﻴﻠﭘ يور ﺎﻫ
يﻮﺘﺤﻣ ي ﺖﻨﻳﺮﺗﻮﻧ
رﺎﮔآ ﻚﺴﻳد و ﺪﻳدﺮﮔ ﻪﻴﻬﺗ و شﺮﺘﺴﮔ ﺎﻫ
ﺎﺑ ﻲﻓﺎﺻ ﺬﻏﺎﻛ ي ﺮﻄﻗ
5 ﺮﺘﻤﻴﻠﻴﻣ يﻮﺘﺤﻣ 250 ﻪﻧﻮﻤﻧ زا مﺮﮔوﺮﻜﻴﻣ ﺎﻫ
ﺖﻴﻠﭘ يور ﺎﻫ
ﺪﺷ هداد راﺮﻗ .
نآ زا ﺲﭘ ﺖﻴﻠﭘ
ﺎﻫ تﺪﻣ ﻪﺑ 24 ﻪﺟرد رد ﺖﻋﺎﺳ
تراﺮﺣ 37 ﺪﻨﺘﻓﺮﮔ راﺮﻗ رﻮﺗﺎﺑﻮﻜﻧا رد داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد .
ﺖﻴﻟﺎﻌﻓ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻪﻧﻮﻤﻧ
ﺎﻫ ﺎﺑ هزاﺪﻧا يﺮﻴﮔ ﺮﻄﻗ ﺎﻫ ﺪﺷر مﺪﻋ ﻪﻟ ﻪﺑ 6
ﺮﺘﻤﻴﻠﻴﻣ ﺪﻳدﺮﮔ ﺖﺒﺛ
. ﻲﺘﻧآ ﻚﻴﺗﻮﻴﺑ راﺪﻘﻣ ﺎﺑ ﻦﻴﻠﻜﻴﺳاﺮﺘﺗ
μg/disc) 30
( ﺑ ﻪ ناﻮﻨﻋ ﺪﻫﺎﺷ ﺖﺒﺜﻣ و
دﺎﻣ
ﺪﺿ ة ﻲﺑوﺮﻜﻴﻣ
ﻊﺟﺮﻣ ﻞﻗاﺪﺣ زا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﻂﺳﻮﺘﻣ و ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد
رﺎﺑ ﻪﺳ هزاﺪﻧا يﺮﻴﮔ ﻪﻧﻮﻤﻧ ياﺮﺑ ﺎﻫ
ﺖﺸﻛ ﻂﻴﺤﻣ يور ﻒﻠﺘﺨﻣ ي
يﺮﺘﻛﺎﺑ ﺎﻫ رﻮﻛﺬﻣ ي ﺑ
ﻪ ﺪﻣآ ﺖﺳد .
زا ﺶﻴﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ 18
ﺑ ﺮﺘﻤﻴﻠﻴﻣ ﻪ
قﻮﻓ ناﻮﻨﻋ ﻦﻴﺑ ،سﺎﺴﺣ هدﺎﻌﻟا
15 - 18 ﺮﺘﻤﻴﻠﻴﻣ
،
ﻦﻴﺑ ،سﺎﺴﺣ ﻂﺳﻮﺘﻣ 10
- 15 ﺮﺘﻤﻴﻠﻴﻣ و ﻒﻴﻔﺧ ﺖﻴﺳﺎﺴﺣ ﺎﺑ
Dimethyl Formamide (DMF)٥
٦
Zone of Inhibition (ZOI)
ﺮﺘﻤﻛ زا 10 ﺮﺘﻤﻴﻠﻴﻣ
، ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد موﺎﻘﻣ .
ﻪﺴﻳﺎﻘﻣ
هوﺮﮔ ﺎﺑ ﻪﻌﻟﺎﻄﻣ درﻮﻣ يﺎﻫ زا هدﺎﻔﺘﺳا
نﻮﻣزآ ﺎﻫ ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ ي
ﻚﻳ و ﻪﻓﺮﻃ نﻮﻣزآ
ﻲﻛﻮﺗ و مﺮﻧ راﺰﻓا Graph Pad Prism
(Graph Pad Software, Inc. USA) )
ﻪﺨﺴﻧ 03 / 4 (
ﺪﺷ مﺎﺠﻧا .
05 / P<0 ﺢﻄﺳ ناﻮﻨﻋ ﻪﺑ ﻲﻨﻌﻣ
راد ي ﺪﻳدﺮﮔ ﻲﻘﻠﺗ .
ﻪﺘﻓﺎﻳ ﺎﻫ
ﻟﺎﻌﻓ ﻴ ﺖ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻪﻧﻮﻤﻧ
ﺎﻫ ي ﻠﻋ ﺮﺑ ﻒﻠﺘﺨﻣ ﻴ
ﻪ
ﺮﺘﻛﺎﺑ ي ﺎﻫ
ﻲﻔﻴﺗ ﻼﻧﻮﻤﻟﺎﺳ
يﻲﻠﻛﺎﻴﺸﻳﺮﺷا
،ﻪﻴﻧﻮﻣﻮﻨﭘ ﻼﻴﺴﺒﻠﻛ
، ،سﻮﺋﺮﺳ سﻮﻠﻴﺳﺎﺑ ﺲﻴﻠﻴﺘﺑﻮﺳ سﻮﻠﻴﺳﺎﺑ
،يﺮﺘﻧﺎﺴﻳد ﻼﮕﻴﺷ
وردﻮﭘ هرﺎﺼﻋ ﻪﻛ داد نﺎﺸﻧ ي
ﻣ ﻴ هﻮ راﺪﻘﻣ ﺎﺑ رﺎﻧا ﻞﻣﺎﻛ 250
ﻣ ﻴ مﺮﮔوﺮﻜ د رد
ﻳ ﻚﺴ ﻲﻣ ﺪﻧاﻮﺗ ﺑ ﻪ ﻔﺧ رﻮﻃ ﻴ ﻒ ﻊﻧﺎﻣ ﺪﺷر
ﻛﺎﺑ ﺮﺘ ي ﺎﻫ
د ﻼﮕﻴﺷ
ييﺮﺘﻧﺎﺴﻳ ﻲﻔﻴﺗ ﻼﻧﻮﻤﻟﺎﺳ
،ﻲﻠﻛ ﺎﻴﺸﻳﺮﺷا
، ،ﻪﻴﻧﻮﻣﻮﻨﭘ ﻼﻴﺴﺒﻠﻛ سﻮﺋﺮﺳ سﻮﻠﻴﺳﺎﺑ
و؛دﻮﺷ ﺗرﻮﺻ رد ﻲ ﻪﻛ
بآ ﻣ ﻴ هﻮ بآ ،رﺎﻧا ﻣ ﻴ هﻮ ﻲﻧﺎﻴﻠﻗ وﺪﻛ هرﺎﺼﻋ و
يردﻮﭘ ﻲﻧﺎﻴﻠﻗ وﺪﻛ
اراد ي ﻟﺎﻌﻓ ﻴ ﺖ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺖﻈﻠﻏ ﺎﺑ
ﺎﻫ ي ﻧ رﻮﻛﺬﻣ ﻲﻤ ﺪﻨﺷﺎﺑ
) لوﺪﺟ 1 ﻞﻜﺷ و 1 (.
ﻞﻜﺷ 1 - ﻪﻧﻮﻤﻧ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻳوﺎﺼﺗ يﺮﺘﻛﺎﺑ ﻪﻴﻠﻋ ﺮﺑ ﺎﻫ
ﻒﻠﺘﺨﻣ يﺎﻫ
:T
ﻦﻴﻠﻜﻴﺳاﺮﺘﺗ :A
بآ ﻲﻧﺎﻴﻠﻗ وﺪﻛ هﻮﻴﻣ
:B
بآ رﺎﻧا هﻮﻴﻣ :C
رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ هرﺎﺼﻋ )
100μl
5 mg/
( :D
ﻲﻧﺎﻴﻠﻗ وﺪﻛ هﻮﻴﻣ يردﻮﭘ هرﺎﺼﻋ )
100μl
5 mg/
(
لوﺪﺟ 1 - رﺎﻴﻌﻣ فاﺮﺤﻧا و ﻦﻴﮕﻧﺎﻴﻣ ﻤﻧ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ
ﻪﻧﻮ ﺮﺘﻤﻴﻠﻴﻣ ﺐﺴﺣ ﺮﺑ ﺎﻫ
ﻪﻧﻮﻤﻧ
ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ
بآ هﻮﻴﻣ ﻲﻧﺎﻴﻠﻗ وﺪﻛ
)
µl/disc
5 ( بآ رﺎﻧا هﻮﻴﻣ
)
µl/disc
5 ( رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ هرﺎﺼﻋ
)
µg/disc
250 ( هرﺎﺼﻋ ﻲﻧﺎﻴﻠﻗ وﺪﻛ
)
µg/disc
250 ( ﻦﻴﻠﻜﻴﺳاﺮﺘﺗ
)
µg/disc
30 (
ﺮﺘﻧﺎﺴﻳد ﻼﮕﻴﺷ ي
33
/
±0 3 / 5 33 /
±0 7 / 6 33 /
±0 3 / 13 58
/
±0 0 / 7 33 /
±0 3 / 20
ﺎﺑ ﺲﻴﻠﻴﺘﺑﻮﺳ سﻮﻠﻴﺳ 33
/
±0 3 / 5 33 /
±0 7 / 5 33 /
±0 7 / 9 33
/
±0 7 / 6 57 /
±0 0 / 20
ﻲﻔﻴﺗ ﻼﻧﻮﻤﻟﺎﺳ
00 /
±0 0 / 5 33 /
±0 3 / 6 33 /
±0 7 / 11 33
/
±0 7 / 7 33 /
±0 3 / 17
ﻲﻠﻛ ﺎﻴﺸﻳﺮﺷا
33 /
±0 7 / 5 00 /
±0 0 / 6 58 /
±0 0 / 11 33
/
±0 7 / 8 88 /
±0 3 / 19
ﻼﻴﺴﺒﻠﻛ ﻪﻴﻧﻮﻣﻮﻨﭘ 33
/
±0 3 / 5 33 /
±0 7 / 5 58 /
±0 0 / 11 33
/
±0 3 / 8 58 /
±0 0 / 19
سﻮﻠﻴﺳﺎﺑ ﺮﺳ ﺋ سﻮ 33 /
±0 3 / 5 68 /
±0 7 / 5 33 /
±0 3 / 10 58
/
±0 0 / 8 58 /
±0 0 / 13
زا ﺶﻴﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ 18
ﺑ ﺮﺘﻤﻴﻠﻴﻣ ﻪ قﻮﻓ ناﻮﻨﻋ ﻦﻴﺑ ،سﺎﺴﺣ هدﺎﻌﻟا 15
- 18 ﺮﺘﻤﻴﻠﻴﻣ
، ﻦﻴﺑ ،سﺎﺴﺣ ﻂﺳﻮﺘﻣ 10
- 15 و ﻒﻴﻔﺧ ﺖﻴﺳﺎﺴﺣ ﺎﺑ ﺮﺘﻤﻴﻠﻴﻣ
زا ﺮﺘﻤﻛ 10 ﺮﺘﻤﻴﻠﻴﻣ
، ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد موﺎﻘﻣ .
هوﺮﮔ مﺎﻤﺗ ﺎﺑ ﻦﻴﻠﻜﻴﺳاﺮﺘﺗ مﺎﻤﺗ رد ﺎﻫ
بوﺮﻜﻴﻣ ﻲﻨﻌﻣ ﺎﻫ دﻮﺑ راد ) 001 / 0
P<
.(
هوﺮﮔ هوﺮﮔ ﺎﺑC A يﺎﻫ
B و ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ مﺎﻤﺗ رد ﻲﻨﻌﻣ ﺎﻫ
دﻮﺑ راد ) 001 /
P<0 .(
هوﺮﮔ هوﺮﮔ ﺎﺑC D يﺮﺘﻛﺎﺑ ﻪﻤﻫ رد يﺮﺘﻛﺎﺑ ﺰﺟ ﺎﻫ
ﻲﻠﻛ ﺎﻴﺸﻳﺮﺷا ﺢﻄﺳ رد
05 /
P<0 ﻲﻨﻌﻣ دﻮﺑ راد
.
ﺚﺤﺑ صاﻮﺧ يور يدﺎﻳز تﺎﻌﻟﺎﻄﻣ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺮﻓ
ا هدرو ﺎﻫ ي
ﺑ ﻲﻌﻴﺒﻃ مﺎﺠﻧا لﺎﺣ رد ﺎﻳ و هﺪﺷ مﺎﺠﻧا نﺎﻫﺎﻴﮔ صﻮﺼﺨ
ﻲﻣ ﺪﺷﺎﺑ . ﺶﻳﺎﻣزآ درﻮﻣ ﻪﻧﻮﻤﻧ رﺎﻬﭼ زا ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد
، هرﺎﺼﻋ
يردﻮﭘ رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ )
و ﻪﻧاد ،ﺖﺳﻮﭘ يﻮﺘﺤﻣ ﭗﻟﺎﭘ
( ياراد ﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻪﻴﻠﻋ ﺮﺑ ﻒﻴﻔﺧ
ﺞﻨﭘ ﺶﺷ زا يﺮﺘﻛﺎﺑ ﻪﻧﻮﮔ ﻪﻧﻮﮔ
ﻲﻨﻌﻳ ﻪﻌﻟﺎﻄﻣ درﻮﻣ
ﺮﺘﻧﺎﺴﻳد ﻼﮕﻴﺷ
ي ﻲﻔﻴﺗ ﻼﻧﻮﻤﻟﺎﺳ
، ﺎﻴﺸﻳﺮﺷا ،ﻲﻠﻛ
ﻼﻴﺴﺒﻠﻛ ،ﻪﻴﻧﻮﻣﻮﻨﭘ ﺮﺳ سﻮﻠﻴﺳﺎﺑ
وﺋ سﻮ
دﻮﺑ . هرﺎﺼﻋ ﻦﻳايور يﺰﻴﭼﺎﻧ ﻲﻠﻴﺧ ﺮﺛا
سﻮﻠﻴﺳﺎﺑ
ﺲﻴﻠﻴﺘﺑﻮﺳ
)يﺮﺘﻛﺎﺑ ﻚﻳ ﻪﻛ
ﻲﻣ ﺖﺒﺜﻣ مﺮﮔ ﺪﺷﺎﺑ
( ﺖﺷاد . ﻦﻳا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﻦﻳﺮﺘﺸﻴﺑ
ﻪﺑ طﻮﺑﺮﻣ هرﺎﺼﻋ
ﺮﺘﻧﺎﺴﻳد ﻼﮕﻴﺷ
ي
) ﻲﻔﻨﻣ مﺮﮔ يﺮﺘﻛﺎﺑ (ﺮﻄﻗ ﺎﺑ
ﻲﺒﻳﺮﻘﺗ 33 /
±0 3 / 13 ﺮﺘﻤﻴﻠﻴﻣ دﻮﺑ . ﻫوﮋﭘ ﺞﻳﺎﺘﻧ Cowan ﺶ
) 21 ( Karou و نارﺎﻜﻤﻫ و
) 22 ( ﻦﻴﺑ ﻲﻃﺎﺒﺗرا ﻪﻛ داد نﺎﺸﻧ
ﻲﻠﭘ تﺎﺒﻴﻛﺮﺗ ﻲﺘﻧآ ﺖﻴﻟﺎﻌﻓ و ﻚﻴﻟﻮﻨﻓ
ﺮﺛا ﺎﺑ ﻲﻧاﺪﻴﺴﻛا
ﺪﺿ
ﻲﻳﺎﻳﺮﺘﻛﺎﺑ دراد دﻮﺟو نﺎﻫﺎﻴﮔ
ﻲﻧاﻮﺨﻤﻫ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ ﺎﺑ ﻪﻛ
؛دراد ﻲﻠﭘ تﺎﺒﻴﻛﺮﺗ ﺮﻳدﺎﻘﻣ اﺮﻳز ﻚﻴﻟﻮﻨﻓ
هزاﺪﻧا يﺮﻴﮔ رد هﺪﺷ
ﻪﻧﻮﻤﻧ ﺎﻫ شور ﺎﺑ
"
ﻮﺘﻟﺎﻛ ﻮﻴﺳ ﻦﻴﻟﻮﻓ
"
ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد 1
نﺎﺸﻧ
ﻲﻠﭘ تﺎﺒﻴﻛﺮﺗ راﺪﻘﻣ ﻪﻛ داد هﻮﻴﻣ هرﺎﺼﻋ رد دﻮﺟﻮﻣ ﻚﻴﻟﻮﻨﻓ
رﺎﻧا ﻞﻣﺎﻛ mg/g)
3 /
±6 00 / 157 ( دوﺪﺣ
ًا ﺮﺑاﺮﺑ ﺶﺷ زا ﺶﻴﺑ
هرﺎﺼﻋ رد تﺎﺒﻴﻛﺮﺗ ﻦﻳا ﻲﻧﺎﻴﻠﻗ وﺪﻛ
mg/g ) 10 /
±1 27 / 25 (
Folin Cio-calteu١
ﻲﻣ ﺪﻨﺷﺎﺑ . ﻪﻛ ﺖﺳا ﺮﻛذ ﻪﺑ مزﻻ ﻲﻧﺎﻫﺎﻴﮔ هرﺎﺼﻋ ﺮﺘﺸﻴﺑ هزوﺮﻣا
يﺮﺘﻛﺎﺑ ﻪﻴﻠﻋ ﺮﺑ يﺎﻫ
يرﺎﻤﻴﺑ از راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ هﺎﮕﺸﻳﺎﻣزآ رد
ﻲﻣ ﻲﻠﭘ تﺎﺒﻴﻛﺮﺗ ياراد ﻪﻛ ﺪﻧﺮﻴﮔ ﺪﻨﺷﺎﺑ يﺮﺗﻻﺎﺑ ﻚﻴﻟﻮﻨﻓ
.
ﻦﻴﻨﭽﻤﻫ ﺖﺳا هﺪﺷ شراﺰﮔ
هرﺎﺼﻋ ﻪﻛ يردﻮﭘ
رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ
ﻲﺘﻧآ ﺖﻴﻟﺎﻌﻓ ياراد ﺖﺒﺴﻧ يﺮﺗﻻﺎﺑ ﻲﻧاﺪﻴﺴﻛا
هرﺎﺼﻋ ﻪﺑ يردﻮﭘ
ﻲﻧﺎﻴﻠﻗ وﺪﻛ ﻲﻣ
ﺪﺷﺎﺑ . و ﻦﻧﺎﺗ يوﺎﺣ رﺎﻧا ﺖﺳﻮﭘ ﺑ
ﻪ ﻲﻌﺒﻨﻣ ناﻮﻨﻋ
ﻲﺘﻧآ زا ناﺪﻴﺴﻛا ﺎﻫ ﻲﻣ ﺪﻨﻧﺎﻣ ﻚﻴﻟﻮﻨﻓ تﺎﺒﻴﻛﺮﺗ و ﺪﺷﺎﺑ
ﺪﻴﺳا ﻻا ﺪﻴﺳا و ﻚﻴﻟﺎﮔ ژ
دراد دﻮﺟو نآ رد ناواﺮﻓ راﺪﻘﻣ ﻪﺑ ﻚﻳ
) 23 .(
ﺪﻨﻳاﺮﻓ ﺎﻫ ﻲﻳ رﻮﻈﻨﻣ ﻪﺑ ﺖﻴﻟﺎﻌﻓ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ تﺎﺒﻴﻛﺮﺗ
ﻲﻠﭘ هﺪﺷ نﺎﻴﺑ ﻚﻴﻟﻮﻨﻓ ﺪﻧا
؛ ﻦﻳا ﻪﻠﻤﺟ زا ﻪﻛ تﺎﺒﻴﻛﺮﺗ ﻦﻳا ﻪﻛ
ﻦﻧﺎﺗ ﺐﻠﻏا ﺎﻫ
هﺪﻴﻣﺎﻧ ﺰﻴﻧ ﻢﻛاﺮﺘﻣ ي ﻲﻣ
ﺪﻧﻮﺷ
، ﻮﻫ رد ﺐﻴﺳآ ا ﺮﻳﺬﭘ
هدﻮﺑ
، ﺶﻨﻛاو ﻖﻳﺮﻃ زا هﺰﻳﺮﻤﻴﻠﭘ نﻮﻴﺳاﺪﻴﺴﻛا يﺎﻫ
ﻲﻣ ﺪﻧﻮﺷ .
ﻞﻨﻓ هﺪﺷﺪﻴﺴﻛا يﺎﻫ
، ﺖﻴﻤﺳ رد ﻲﺸﻘﻧ ﺖﺳا ﻦﻜﻤﻣ
ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺪﻨﺷﺎﺑ ﻪﺘﺷاد ﺎﻫ
. يﺮﺘﻛﺎﺑ رﺎﻬﻣ تﺎﺒﻴﻛﺮﺗ ﻂﺳﻮﺗ ﺎﻫ
يﺎﻫﺪﻧﺎﺑ دﺎﺠﻳا ﺎﻳ ﻦﻫآ ﺶﻫﺎﻛ ﻪﺑ طﻮﺑﺮﻣ ﺖﺳا ﻦﻜﻤﻣ ﻚﻴﻟﻮﻨﻓ ﻦﻴﺌﺗوﺮﭘ ﺎﺑ ﻲﻧژورﺪﻴﻫ ﺎﻫ
بوﺮﻜﻴﻣ ياﺮﺑ ﻲﺗﺎﻴﺣ ي ﺪﻨﻧﺎﻣ
ﻢﻳﺰﻧآ ﺪﺷﺎﺑ ﻲﺑوﺮﻜﻴﻣ يﺎﻫ )
25،24 Cowan .(
ﺮﺛا ﻪﻛ دﺮﻛ ﺮﻛذ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻲﺘﻧآ تﺎﺒﻴﻛﺮﺗ ﻦﻳا
رﺎﻬﻣ ﻪﺑ طﻮﺑﺮﻣ ناﺪﻴﺴﻛا
ﻢﻳﺰﻧآ يﺮﺘﻛﺎﺑ رد ﻚﻴﺘﻴﻟورﺪﻴﻫ يﺎﻫ ﻲﻣ
ﺪﺷﺎﺑ ) 21 .(
تﺎﻘﻴﻘﺤﺗ
يﺮﺘﺸﻴﺑ ياﺮﺑ
ﺺﻟﺎﺧ و يزﺎﺳاﺪﺟ ﻲﻠﭘ تﺎﺒﻴﻛﺮﺗ يزﺎﺳ
ﻚﻴﻟﻮﻨﻓ
تاﺮﺛا ﻦﻴﻴﻌﺗ و رﺎﻧا رد دﻮﺟﻮﻣ
ﺪﺿ ﻳﺮﺘﻛﺎﺑ ﻲﻳﺎ ﻪﻴﻠﻋ ﺮﺑ ﺎﻬﻧآ
يﺮﺘﻛﺎﺑ ﺎﻫ ﻮﺗﺎﭘ ي ژ ﻒﻠﺘﺨﻣ ن )
ﻲﻔﻨﻣ مﺮﮔ و ﺖﺒﺜﻣ مﺮﮔ (
زﺎﻴﻧ درﻮﻣ
ﺎﺗ ﺖﺳا ﻲﺘﻧآ ﻚﻴﺗﻮﻴﺑ ارﺎﻛ ﺎﺑ ﻲﻳﺎﻫ ﻳ
ددﺮﮔ ﻪﻴﻬﺗ ﻻﺎﺑ ﻲ .
ﻪﺠﻴﺘﻧ يﺮﻴﮔ
،ﻖﻴﻘﺤﺗ ﻦﻳا ﺞﻳﺎﺘﻧ سﺎﺳا ﺮﺑ ﻞﻣﺎﻛ هﻮﻴﻣ هرﺎﺼﻋ
ﺎﺑ رﺎﻧا
راﺪﻘﻣ 250 ﺮﺛا ياراد ﻚﺴﻳد ﺮﻫ رد مﺮﮔوﺮﻜﻴﻣ
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ
ﻒﻴﻔﺧ ﻲﻣ ﺪﺷﺎﺑ
؛ رد ﻲﺗرﻮﺻ ﻪﻛ ﻲﻧﺎﻴﻠﻗ وﺪﻛ )
بآ و هﻮﻴﻣ هرﺎﺼﻋ
يردﻮﭘ ( و رﺎﻧا هﻮﻴﻤﺑآ ﻦﻴﻨﭽﻤﻫ تاﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﻪﻴﻠﻋ ﺮﺑ
يﺮﺘﻛﺎﺑ ﺎﻫ ﺪﻨﺘﺷاﺪﻧ ار رﻮﻛﺬﻣ ي .
ﺎﺗ ﺖﺳا زﺎﻴﻧ يﺮﺘﺸﻴﺑ تﺎﻌﻟﺎﻄﻣ
تاﺮﺛا
ﺪﺿ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺎﺑ رﺎﻧا ﻞﻣﺎﻛ هﻮﻴﻣ
شور زا هدﺎﻔﺘﺳا ﺎﻫ
ي هرﺎﺼﻋ ﺮﮕﻳد يﺮﻴﮔ
لﻼﺣ و ﺎﻫ ﺎﺑ ﻦﻴﻨﭽﻤﻫ و ﻒﻠﺘﺨﻣ ي ﺎﻔﺘﺳا
هد
ﺖﻈﻠﻏ زا ﺎﻫ
ﺑ هرﺎﺼﻋ ﺖﻈﻠﻏ ﺮﺜﻛاﺪﺣ زا ﺮﺗﻻﺎﺑ ي ﻪ
هﺪﺷ هدﺮﺑ رﺎﻛ
ﻖﻴﻘﺤﺗ ﻦﻳا رد دﺮﻳﺬﭘ ترﻮﺻ
.
ﺮﻜﺸﺗ و ﺮﻳﺪﻘﺗ
ﻲﻟﺎﻣ ﺖﻳﺎﻤﺣ ﺎﺑ ﻖﻴﻘﺤﺗ ﻦﻳا ﺎﻧﻮﭘ هﺎﮕﺸﻧاد
) نﺎﺘﺳوﺪﻨﻫ (
و
يرﺎﻜﻤﻫ ﻦﻴﻨﭽﻤﻫ ﻲﻤﻴﺷﻮﻴﺑ هﺎﮕﺸﻳﺎﻣزآ نﺎﻨﻛرﺎﻛ
ﻦﻳا هﺎﮕﺸﻧاد
نﺎﻳﺎﻗآ صﻮﺼﺨﺑ Yogish
Sharad و هرود نﺎﻳﻮﺠﺸﻧاد
ﺎﺠﻧا ﻪﺑ Ph.D ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ ﺐﺗاﺮﻣ نﺎﮔﺪﻨﺴﻳﻮﻧ ؛ﺪﻴﺳر م
ﻲﻣ مﻼﻋا ار دﻮﺧ ﺪﻧراد
.
ﻊﺑﺎﻨﻣ :
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Abstract Original Article
Evaluation of antibacterial activity of fruit juices and whole fruit powder extract of pomegranate and bottle gourd
on gram positive and negative bacterias
N. Ghodratollah1, M. Hassanpour-Fard2, S. Bodhankar3, M. Dikshit4
Background and Aim: Treatment with antibiotics is accompanied by difficulties, such as unwanted side effects and drug resistance. Medicinal plants can be considered as suitable alternative, because they may have fewer side effects. Pomegranate and bottle gourd are fruits that are easily available in many areas of the world. The current study was aimed to determine the antibacterial effect of these nutrients.
Materials and Methods: In this experimental study, the antibacterial activity of whole fruit powder extract of skinless pomegranate and bottle gourd and their fruit juices, were determined by the disc plate method against six different bacterial species, including salmonella typhi, Escherichia coli, Klebsiella pneumonia, Bacillus cereus, Bacillus Subtilis and Shigella dysentery. The diameter of zone of inhibition was measured by adding the samples and tetracycline (as reference) in the culture medium containing each bacterium (at least 3 times for each sample). To compare the groups, ANOVA and Tukey's tests were used at the significant level of P<0.05.
Results: Powder extract of pomegranate (250 µg/disc) could stop the growth of the studied bacteria (except Bacillus Subtilis) mildly, whereas the fruit juices of pomegranate and bottle gourd and the powder extract of bottle gourd did not show antibacterial activity.
Conclusion: The mild antibacterial activity of powder extract of pomegranate may be related to its more polyphenolic compounds compared to bottle gourd.
Key Words: Pomegranate, Bottle gourd, Antibacterial activity, Tetracycline
Journal of Birjand University of Medical Sciences. 2011; 17(4):257-264 Received: 08.06.2010 Last Revised: 06.03.2011 Accepted: 07.03.2011
1 Corresponding Author; Assistant Professor, Department of Surgery, Imam Reza Hospital, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran [email protected]
2Assistant Professor, Department of Pharmacology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran
3 Professor, Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India
4 Professor, Biochemistry Division, Department of Chemistry, University of Pune, Pune, India
