و ﻲﻠﻛﺎﻴﺷﺮﺷا يور ﺮﺑ يرﺎﻣزر ﺲﻧﺎﺳا ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﺿ صاﻮﺧ و ﻲﻳﺎﻴﻤﻴﺷ ﺐﻴﻛﺮﺗ ﻲﺳرﺮﺑ نآ ﻚﻴﺘﻨﻴﺳ ﻦﻴﻴﻌﺗ
نﺎﮔﺪﻨﺴﻳﻮﻧ :
نﺎﻴﺗﻮﻜﻠﻣ ﺪﻤﺤﻣ ﻲﻤﺗﺎﺣ مﺎﻨﻬﺑ1
2
1.
لﻮﺌﺴﻣ هﺪﻨﺴﻳﻮﻧ :
هوﺮﮔو ﻂﻴﺤﻣ ﺖﺷاﺪﻬﺑ تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ دﺎﺘﺳا ﻂﻴﺤﻣ ﺖﺷاﺪﻬﺑ
نﺎﻣﺮﻛ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،
Email: [email protected] 2 . ﻪﺘﺧﻮﻣآ ﺶﻧاد سﺎﻨﺷرﺎﻛ
ﻂﻴﺤﻣ ﺖﺷاﺪﻬﺑ ﻲﺳﺪﻨﻬﻣ ﺪﺷرا
، نﺎﻣﺮﻛ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد
ﺖﺷاﺪﻬﺑ عﻮﻠﻃ
ﻲﺸﻫوﮋﭘ ﻲﻤﻠﻋ ﺔﻣﺎﻨﻠﺼﻓ دﺰﻳ ﺖﺷاﺪﻬﺑ هﺪﻜﺸﻧاد
ﻢﻫدزاود لﺎﺳ
هرﺎﻤﺷ : لوا
رﺎﻬﺑ 2 139
ﻞﺴﻠﺴﻣ هرﺎﻤﺷ :
38
لﻮﺻو ﺦﻳرﺎﺗ :
28 / 6 / 91
شﺮﻳﺬﭘ ﺦﻳرﺎﺗ :
8 / 9 / 91
هﺪﻴﻜﭼ
ﻪﻣﺪﻘﻣ ﻪﺑ : ﻪﺑ ﻲﺑﺎﻴﺘﺳد ﺖﻬﺟ ﻲﻳوراد نﺎﻫﺎﻴﮔ زا هدﺎﻔﺘﺳا ،ﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﺮﺑاﺮﺑ رد ﺎﻬﻤﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺖﻣوﺎﻘﻣ ﺖﻠﻋ
ﺪﺷﺎﺑ ﻲﻣ يروﺮﺿ و ﻢﻬﻣ يﺮﻣا ،ﺎﻬﻧآ ﺮﺑ ﻪﺒﻠﻏ و ﺪﻳﺪﺟ تﺎﺒﻴﻛﺮﺗ .
فﺪﻫ زا مﺎﺠﻧا ﻦﻳا
،ﻪﻌﻟﺎﻄﻣ ﺮﺛﻮﻣ تﺎﺒﻴﻛﺮﺗ ﻲﺳرﺮﺑ
ﻲﻜﻳ ناﻮﻨﻋ ﻪﺑ ،ﻲﻠﻛﺎﻴﺷﺮﺷا يور ﺮﺑ يرﺎﻣزر ﺲﻧﺎﺳا ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﺿ صاﻮﺧ و و اﺰﻳرﺎﻤﻴﺑ يﺎﻬﻳﺮﺘﻛﺎﺑ ﻦﻳﺮﺘﻌﻳﺎﺷ زا
ﺪﺷﺎﺑ ﻲﻣ نآ ﺮﺛا ﻚﻴﺘﻨﻴﺳ ﻦﻴﻴﻌﺗ .
ﻲﺳرﺮﺑ شور يزﺎﺳاﺪﺟ زا ﺲﭘ :
هﺎﻴﮔ يرﺎﻣزر زا ﺮﮕﻳد نﺎﻫﺎﻴﮔ ﻢﻴﻠﻗا نﺎﻣﺮﻛ ﺮﻬﺷ ،نآ ندﻮﻤﻧ ﻚﺸﺧ و
ﺪﻳدﺮﮔ مﺎﺠﻧا بآ ﺎﺑ ﺮﻴﻄﻘﺗ شور ﻪﺑ ﺲﻧﺎﺳا جاﺮﺨﺘﺳا .
ﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛزﺎﮔ ﻪﻠﻴﺳﻮﺑ ،هﺪﻣآ ﺖﺳﺪﺑ ﺲﻧﺎﺳا ﻲﻣﺮﺟ
ﻣ تﺎﺒﻴﻛﺮﺗ و ﺰﻴﻟﺎﻧآ ﺪﺷ ﻲﻳﺎﺳﺎﻨﺷ نآ رد دﻮﺟﻮ
. رﺎﺸﺘﻧا دراﺪﻧﺎﺘﺳا يﺎﻬﺷور زا ،ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﺿ صاﻮﺧ ﻦﻴﻴﻌﺗ ﺖﻬﺟ
ﺪﺷ هدﺎﻔﺘﺳا ﻲﮔﺪﻨﺸﻛ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﻴﻴﻌﺗ و ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﻴﻴﻌﺗ ،ﻚﺴﻳد .
ﺎﻨﻤﺿ ﺮﺛا ﻚﻴﺘﻨﻴﺳ
ﺲﻧﺎﺳا ﺮﺑ ﺪﻳدﺮﮔ ﻦﻴﻴﻌﺗ ﺰﻴﻧ يﺮﺘﻛﺎﺑ يور .
ﺎﻫ ﻪﺘﻓﺎﻳ رد: ،يرﺎﻣزر ﺲﻧﺎﺳا ﺰﻴﻟﺎﻧآ 20
ﻲﻳﺎﺳﺎﻨﺷ ﺐﻴﻛﺮﺗ ﻪﻛ ﺪﺷ
09 / 82 ﻞﻣﺎﺷ تﺎﺒﻴﻛﺮﺗ ﻦﻳا ﺪﺻرد 1,8
Cineole ، Borneo ، -pinen ،α Camphor ،
Linalool ،
Camphene ،
limonene و
Verbenone ﺪﺷﺎﺑ ﻲﻣ
.
ﺖﻈﻠﻏ رد هﺪﻣآ ﺖﺳﺪﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ µg/Disc
32 يﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ زا ،ﺲﻧﺎﺳا
را و ﻦﻴﺴﻳﺎﻣﻮﺘﭘﺮﺘﺳا ،ﻦﻴﻠﻴﺳ ﻲﻨﭘ ،ﻦﻴﺴﻳﺎﻣﺎﺘﻨﺟ ﺪﺷﺎﺑ ﻲﻣ ﺮﺘﺸﻴﺑ ﻦﻴﺴﻳﺎﻣوﺮﺘﻳ
. ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﻴﻨﭽﻤﻫ
ﺪﺷر زا ) (MIC ﻲﮔﺪﻨﺸﻛ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و )
(MBC ﺮﺑاﺮﺑ ﺐﻴﺗﺮﺗ ﻪﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد
ஜ
3000
ஜ و 3200
ﺪﺷ ﻞﺻﺎﺣ .
ﺰﻴﻧ ﻲﻠﻛﺎﻴﺷﺮﺷا ﻞﻣﺎﻛ يدﻮﺑﺎﻧ ياﺮﺑ زﺎﻴﻧ درﻮﻣ نﺎﻣز 25
ﺪﻣآ ﺖﺳﺪﺑ ﻪﻘﻴﻗد .
يﺮﻴﮔ ﻪﺠﻴﺘﻧ ﻲﺳرﺮﺑ :
يﺎﻫ مﺎﺠﻧا ﻪﺘﻓﺮﮔ رد ﻦﻳا ﺮﻬﺷ زا هﺪﺷ يروآ ﻊﻤﺟ يرﺎﻣزر هﺎﻴﮔ ﺲﻧﺎﺳا داد نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ
ﻲﺘﻧآ ﻲﺧﺮﺑ ﻪﺑ ﺖﺒﺴﻧ ﻪﻜﻳرﻮﻄﺑ ،هدﻮﺑ ﻲﻠﻛﺎﻴﺷﺮﺷا ﻪﻴﻠﻋ ﺮﺑ ﻲﺑﻮﺧ رﺎﻴﺴﺑ ﻲﻳﺎﻳﺮﺘﻛﺎﺑﺪﺿ ﺖﻴﺻﺎﺧ ياراد نﺎﻣﺮﻛ ﺪﻫد ﻲﻣ نﺎﺸﻧ ،يﺮﺘﻤﻛ ﺖﻈﻠﻏ رد ار ﺖﻴﺻﺎﺧ ﻦﻳا ،ﺞﻳار ﻲﻳوراد يﺎﻬﻜﻴﺗﻮﻴﺑ .
يﺪﻴﻠﻛ يﺎﻫ هژاو ﺮﺷا ،ﺲﻧﺎﺳا ،يرﺎﻣزر :
ﻲﺑوﺮﻜﻴﻣﺪﺿ ،ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ،ﻲﻠﻛﺎﻴﺷ
ﻪﻣﺪﻘﻣ ﻲﻳوراد ﻒﻠﺘﺨﻣ يﺎﻫ ﻪﻨﻴﻣز رد ﻲﻜﺷﺰﭘ دﺪﻌﺘﻣ تﺎﻓﺎﺸﺘﻛا دﻮﺟو ﺎﺑ داﺪﻌﺗ ﺶﻳاﺰﻓا هﺪﻨﻫد نﺎﺸﻧ رﺎﻣآ ،نﺎﻣرد ﻪﺘﻓﺮﺸﻴﭘ يﺎﻬﺷور و ﻲﻧﻮﻔﻋ يﺎﻬﻳرﺎﻤﻴﺑ )
Infectious Diseases (
رد يﺎﻫرﻮﺸﻛ رد
لﺎﺣ ﺪﺷﺎﺑ ﻲﻣ ﻪﻌﺳﻮﺗ )
1 .(
يرﺎﻴﺴﺑ زا ﻦﻳا يﺎﻬﻳرﺎﻤﻴﺑ ﻲﻧﻮﻔﻋ
ﻪﻄﺳاﻮﺑ
يﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ اﺰﻳرﺎﻤﻴﺑ
ﺪﻨﻧﺎﻣ يﺮﺘﻛﺎﺑ
،ﺎﻫ
،ﺎﻬﺳوﺮﻳو
،ﺎﻬﭼرﺎﻗ
ازﻮﺗوﺮﭘ و ﺎﻬﻠﮕﻧا دﺎﺠﻳا ﻲﻣ ﺪﻧﻮﺷ و ﺖﻴﻠﺑﺎﻗ لﺎﻘﺘﻧا زا ﻚﻳ ﺺﺨﺷ
ﻪﺑ ﺺﺨﺷ ﺮﮕﻳد ار اراد ﻲﻣ ﺪﻨﺷﺎﺑ ) 2 .(
،نﺎﻴﻣ ﻦﻳا رد ﺶﺨﺑ
هﺪﻤﻋ
يا زا عﻮﻴﺷ يرﺎﻤﻴﺑ و گﺮﻣ و ﺮﻴﻣ رد ﺎﻬﻧﺎﺴﻧا ﻪﺑ يﺎﻬﻳرﺎﻤﻴﺑ ﻲﺷﺎﻧ
زا يﺮﺘﻛﺎﺑ
،ﺎﻫ ﺖﺒﺴﻧ هداد ﻲﻣ دﻮﺷ ﺪﻨﻧاﻮﺗ ﻲﻣ ﻪﻛ زا
قﺮﻃ ﻒﻠﺘﺨﻣ
ﺪﻨﻧﺎﻣ
،ﻲﻧﺪﻴﻣﺎﺷآ بآ ﺲﻔﻨﺗ
و داﻮﻣ ﻲﻳاﺬﻏ دراو نﺪﺑ نﺎﺴﻧا
ﺪﻧﻮﺷ ) 2,3 .(
ﻴﺑ يﺎﻬﻳﺮﺘﻛﺎﺑ ﻦﻳﺮﺘﻌﻳﺎﺷ زا ﻲﻜﻳ ـ
اﺰﻳرﺎﻤ ﺷﺮﺷا ـ ﻠﻛﺎﻴ ـــ ﻲ
) Escherichia coli (
يﺎﻫاﺬﻏ و هدﻮﻟآ بآ ﻖﻳﺮﻃ زا ﻪﻛ هدﻮﺑ
ﺖﻴﻣﻮﻤﺴﻣ ﺚﻋﺎﺑ ﻪﺘﺨﭘﺎﻧ دﺎﺣ دراﻮﻣ رد و ﻲﺷراﻮﮔ تﻻﻼﺘﺧا ،
دﻮﺷ ﻲﻣ ﺮﻴﻣ و گﺮﻣ ﺚﻋﺎﺑ )
2 .(
ﻲﻔﻠﺘﺨﻣ يﺎﻬﻳرﺎﻤﻴﺑ عﻮﻴﺷ نﻮﻨﻛﺎﺗ
ﻲﻳاﺬﻏ داﻮﻣ و ﻲﻧﺪﻴﻣﺎﺷآ يﺎﻬﺑآ رد ﻲﻠﻛﺎﻴﺷﺮﺷا رﻮﻀﺣ زا ﻲﺷﺎﻧ ﻪﺑ ناﻮﺗ ﻲﻣ ﻪﻛ هﺪﺷ شراﺰﮔ نﺎﻬﺟ ﻒﻠﺘﺨﻣ طﺎﻘﻧ رد نﻮﺗﺮﻜﻟاو ) Walkerton (
ﺪﻨﻠﻳﺎﻫ ،ادﺎﻧﺎﻛ رد )
Highland (
رد
ﺎﻛﺎﺳوا و ﺪﻨﻠﺗﺎﻜﺳا )
Osaka ( دﻮﻤﻧ هرﺎﺷا ﻦﭘاژ رد )
4,5 .(
ﻦﻴﻨﭽﻤﻫ
ﻲﻳاﺬﻏ داﻮﻣ رد ﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺪﺷر و رﻮﻀﺣ ﺖﺳا ﻦﻜﻤﻣ
ﺖﻴﻔﻴﻛ و ﺖﻴﻤﻛ ﺶﻫﺎﻛ ﻪﺠﻴﺘﻧ رد و دﺎﺴﻓ ﺚﻋﺎﺑ ﺰﻴﻧ نآ
ددﺮﮔ ) 6,7 ( . يﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ و ﻲﻳاﺬﻏ داﻮﻣ دﺎﺴﻓ لﺮﺘﻨﻛ
ﺎﺗﺪﻤﻋ اﺰﻳرﺎﻤﻴﺑ دﻮﺷ ﻲﻣ مﺎﺠﻧا ﻲﻳﺎﻴﻤﻴﺷ ﻞﻣاﻮﻋ ﻪﻠﻴﺳﻮﺑ
. ﺖﻠﻋ ﻪﺑ ﺎﻣا
،ﻪﻳﺰﺠﺗ نﺎﻣز ندﻮﺑ ﻲﻧﻻﻮﻃ و ﻲﻳاز ﺺﻗﺎﻧ ،ﻲﻳاز نﺎﻃﺮﺳ صاﻮﺧ ﻲﻳﺎﻴﻤﻴﺷ ﻞﻣاﻮﻋ زا هدﺎﻔﺘﺳا ﺪﺷﺎﺑ ﻲﻣ دوﺪﺤﻣ
) 8 .(
ﻲﻳوراد صاﻮﺧ ﻪﻄﺳاو ﻪﺑ ﻲﻳوراد نﺎﻫﺎﻴﮔ ،نﻮﻨﻛ ﺎﺗ زﺎﺑﺮﻳد زا
ﻨﺳ ﺐﻃ رد هدﺮﺘﺴﮔ رﻮﻃ ﻪﺑ ،ﺎﻬﻧآ تﺎﺒﻴﻛﺮﺗ رد دﻮﺟﻮﻣ و ﻲﺘ
ﻳﻮﻧ ﻲﻜﺷﺰﭘ ﺪﻧﻮﺷ ﻲﻣ هدﺎﻔﺘﺳا ﻦ
) 9 (.
ﻲﺘﻧآ ﻲﺒﻧﺎﺟ تاﺮﺛا ﺖﻠﻋ ﻪﺑ
،ﺎﻬﻧآﺮﺑاﺮﺑ رد ﺎﻬﻤﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺖﻣوﺎﻘﻣ ﺶﻳاﺰﻓا و ﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ يﺎﻬﺘﻧﻮﻔﻋ دﻮﺒﻬﺑ رد يدﺎﻳز ﺖﻴﺑﻮﺒﺤﻣ زا ﻲﻳوراد نﺎﻫﺎﻴﮔ ﺪﻨﺷﺎﺑ ﻲﻣ رادرﻮﺧﺮﺑ )
2 .(
تﺎﺒﻴﻛﺮﺗ ﻲﺳرﺮﺑ ﻪﺑ ،نﺎﻘﻘﺤﻣ ور ﻦﻳا زا
ﺎﻴﮔ ﻒﻠﺘﺨﻣ يﺎﻫ ﻪﻧﻮﮔ زا هﺪﺷ جاﺮﺨﺘﺳا لﺎﻌﻓ ﻦﻴﺑ زا ﺖﻬﺟ ﻲﻫ
ﺪﻨﺷﺎﺑ ﻲﻣ ﺪﻨﻤﻗﻼﻋ اﺰﻳرﺎﻤﻴﺑ يﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ندﺮﺑ )
10 .(
ﺎﻬﺴﻧﺎﺳا ﺪﻨﻧﺎﻣ هﺎﻴﮔ ﻪﻳﻮﻧﺎﺛ يﺎﻫ ﺖﻴﻟﻮﺑﺎﺘﻣ )
Essential Oils (
و
ﺎﻫﺪﻴﺋﻮﻧووﻼﻓ )
flavonoids (
ﺪﺿ ،ﻲﺑوﺮﻜﻴﻣ ﺪﺿ صاﻮﺧ ﺖﻠﻋ ﻪﺑ
درﻮﻣ هدﺮﺘﺴﮔ رﻮﻃ ﻪﺑ ﻲﻧاﺪﻴﺴﻛا ﻲﺘﻧآ و ﻲﭼرﺎﻗ ﺪﺿ ،ﻲﻳﺎﻳﺮﺘﻛﺎﺑ راﺮﻗ هدﺎﻔﺘﺳا ﺪﻧا ﻪﺘﻓﺮﮔ
) 3,11,12,13,14,15,16 .(
يرﺎﻣزر هﺎﻴﮔ )
Rosmarinus officinalis (
هداﻮﻧﺎﺧ ﻪﺑ ﻖﻠﻌﺘﻣ
نﺎﻴﻋﺎﻨﻌﻧ ) Lumiaceae (
ﺪﺿ ،ﻲﺑوﺮﻜﻴﻣ ﺪﺿ صاﻮﺧ ﺖﻠﻋ ﻪﺑ
ﻲﻳوراد ﻊﻳﺎﻨﺻ رد ،ﻲﻳﺎﻴﻤﻴﺷ هﺪﻨﻨﻛ يﺮﻴﮕﺸﻴﭘ ﻞﻣﺎﻋ و ﻲﻳاز ﺶﻬﺟ هﺎﻴﮔ ﻚﻳ ناﻮﻨﻋ ﻪﺑ ﻲﻜﺷﺰﭘ و هﺪﺷ ﻪﺘﺧﺎﻨﺷ شزرا ﺎﺑ ﻲﻳوراد
ﺖﺳا ) 9,12,17,18,19 (.
ﺲﻧﺎﺳا يدﺎﻳز راﺪﻘﻣ يوﺎﺣ يرﺎﻣزر
) زا ﺶﻴﺑ 1 (%
رد ﻲﻳاﺬﻏ داﻮﻣ رد هﺪﻨﻫد ﻢﻌﻃ ناﻮﻨﻋ ﻪﺑ ﻪﻛ هدﻮﺑ
ﻲﺸﻳارآ ﻊﻳﺎﻨﺻ ﺎﻬﺸﻛ هﺮﺸﺣ و ﺎﻫادزﺪﻨﮔ تﺎﺒﻴﻛﺮﺗ رد ﻦﻴﻨﭼ ﻢﻫ و
دﻮﺷ ﻲﻣ هدﺎﻔﺘﺳا )
9,11,12,20 (
. ﺲﻧﺎﺳا يرﺎﻣزر هﺎﻴﮔ
، شدﺮﮔ
ﺪﺿ تاﺮﺛا ياراد ،هداد ﺶﻳاﺰﻓا ار نﺪﺑ يﺎﻫﻮﻀﻋ رد نﻮﺧ ﺪﻫد ﻲﻣ ﺶﻫﺎﻛ ار ﻲﺒﺼﻋ يﺎﻫدرد و هدﻮﺑ ﻲﻤﺴﻴﺗﺎﻣور )
17 ( . ﻦﻳا
زا ﻲﻨﻏ ﻲﻌﺒﻨﻣ هﺎﻴﮔ ﻻﺎﺑ ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ ﺎﺑ ﻲﻠﻨﻓ تﺎﺒﻴﻛﺮﺗ
ﻲﻔﻨﻣ مﺮﮔ و ﺖﺒﺜﻣ مﺮﮔ يﺎﻫ يﺮﺘﻛﺎﺑ ﻪﻴﻠﻋ ﺪﺷﺎﺑ ﻲﻣ
) 9 ( . تﺎﻌﻟﺎﻄﻣ
ﺧ يور ﺮﺑ ﻲﻧﻮﮔﺎﻧﻮﮔ ـ
ﻜﻴﻣ ﺪﺿ صاﻮ ـ
ﮔ ﻲﺑوﺮ ـ ﺨﻣ يﺎﻫ ﻪﻧﻮ ــ
ﻒﻠﺘ
ﺖﺳا هﺪﺷ مﺎﺠﻧا نآ هﺪﻨﻫد ﻞﻴﻜﺸﺗ تﺎﺒﻴﻛﺮﺗ و يرﺎﻣزر .
ﻪﻠﻤﺟ زا
ﻪﻌﻟﺎﻄﻣ Keskin ﺪﺿ صاﻮﺧ ﻪﻛ ﻪﻴﻛﺮﺗ رد ﺶﻧارﺎﻜﻤﻫ و
يور ﺮﺑ ار يرﺎﻣزر ﻪﻠﻤﺟ زا نﻮﮔﺎﻧﻮﮔ نﺎﻫﺎﻴﮔ ﻲﺑوﺮﻜﻴﻣ ﻪﻌﻟﺎﻄﻣ ﺎﻳ و ﺪﻧدﻮﻤﻧ ﻲﺳرﺮﺑ ﻒﻠﺘﺨﻣ نژﻮﺗﺎﭘ يﺎﻬﻳﺮﺘﻛﺎﺑ Pintore
و
ود رد هﺪﺷ هﺪﻴﺋور يرﺎﻣزر ﻲﺑوﺮﻜﻴﻣ ﺪﺿ صاﻮﺧ ﻪﻛ ﺶﻧارﺎﻜﻤﻫ راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ ار ﺎﻴﻟﺎﺘﻳا توﺎﻔﺘﻣ ﻲﻳاﻮﻫ و بآ ﻪﻘﻄﻨﻣ ﺪﻧداد ) 6,11,21 ( . يرﺎﻣزر ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ ﻪﭼﺮﮔا ﻂﺳﻮﺗ
ﻲﺳرﺮﺑ درﻮﻣ ﻒﻠﺘﺨﻣ طﺎﻘﻧ رد ﻦﻴﻘﻘﺤﻣ ﺎﺑ ﺎﻣا ،ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ
ﺛﺎﺗ ﻞﻣاﻮﻋ ﻪﺑ ﻪﺟﻮﺗ ﺖﻴﺻﺎﺧ ﻪﺠﻴﺘﻧ رد و ﺲﻧﺎﺳا ﺐﻴﻛﺮﺗ ﺮﺑ راﺬﮔ ﺮﻴ
يﺎﻬﻧﺎﻜﻣ رد يرﺎﻣزر ﺲﻧﺎﺳا ﺖﻴﺻﺎﺧ ﻪﻌﻟﺎﻄﻣ ،ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺪﺳر ﻲﻣ ﺮﻈﻧ ﻪﺑ يروﺮﺿ توﺎﻔﺘﻣ ياﻮﻫ و بآ ﺎﺑ توﺎﻔﺘﻣ )
18 ( .
ﺲﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ ﺪﺿ صاﻮﺧ ﻦﻴﻴﻌﺗ ،ﻪﻌﻟﺎﻄﻣ ﻦﻳا زا فﺪﻫ ﺮﺑ نﺎﻣﺮﻛﺮﻬﺷ رد هﺪﺷ يروآ ﻊﻤﺟ يرﺎﻣزر زا هﺪﺷ جاﺮﺨﺘﺳا ﻪﻠﻴﺳﻮﺑ يﺮﺘﻛﺎﺑ گﺮﻣ ﻚﻴﺘﻨﻴﺳ ﻦﻴﻴﻌﺗ ﻦﻴﻨﭽﻤﻫ و ﻲﻠﻛﺎﻴﺷﺮﺷا يور ﺪﺷﺎﺑ ﻲﻣ ﺪﺷر ﻲﻨﺤﻨﻣ ﻢﺳر ﻖﻳﺮﻃ زا ﺲﻧﺎﺳا .
ﻲﺳرﺮﺑ شور
ﺲﭘ و يروآ ﻊﻤﺟ نﺎﻣﺮﻛ ﺮﻬﺷ ﻒﻠﺘﺨﻣ يﺎﻬﺘﻤﺴﻗ زا يرﺎﻣزر هﺎﻴﮔ ا ناﺮﻳا ﻲﻫﺎﻴﮔ ﻪﭘﻮﻛﺎﻣرﺎﻓ سﺎﺳا ﺮﺑ ﻲﺳﺎﻨﺷ هﺎﻴﮔ يﺎﻬﻧﻮﻣزآ مﺎﺠﻧا ز هداد ﺖﻘﺑﺎﻄﻣ ﺰﻴﻧ يزﺎﺳوراد هﺪﻜﺸﻧاد مﻮﻳرﺎﺑﺮﻫ ﺎﺑ ،نآ يور ﺮﺑ
ﺪﺷ . هﺎﻴﮔ يﺎﻣد ﺮﺛا زا يﺮﻴﮔﻮﻠﺟ ﺖﻬﺟ ،ﻪﻴﻟوا يﻮﺸﺘﺴﺷ زا ﺲﭘ
يﺎﻣد رد ﻪﺘﻔﻫ ﻚﻳ تﺪﻣ ﻪﺑ ،نآ تﺎﺒﻴﻛﺮﺗ يور ﺮﺑ ﻻﺎﺑ
ﺪﻳدﺮﮔ بﺎﻴﺳآ ﺲﭙﺳ و هﺪﺷ ﻚﺸﺧ ﻂﻴﺤﻣ .
جاﺮﺨﺘﺳا
بآ ﺎﺑ ﺮﻴﻄﻘﺗ شور ﻪﺑ )
Hydrodistillation (
هﺎﮕﺘﺳد ﻪﻠﻴﺳﻮﺑ
ﺮﺠﻧﻮﻠﻛ ) Clevenger (
ﺪﺷ مﺎﺠﻧا . 250 هﺪﺷ ﻚﺸﺧ هﺎﻴﮔ مﺮﮔ
رد 1600 تﺎﻴﻠﻤﻋ ﺖﻋﺎﺳ ﻪﺳ تﺪﻣ ﻪﺑ هﺪﺷ ﺮﻴﻄﻘﺗ بآ ﺮﺘﻴﻟ ﻲﻠﻴﻣ
ﺪﻳدﺮﮔ مﺎﺠﻧا جاﺮﺨﺘﺳا .
ﻲﻓﺎﺻ زا ﻞﺻﺎﺣ طﻮﻠﺨﻣ رﻮﺒﻋ زا ﺲﭘ ﻦﻤﺗاو
) Whatman (
هرﺎﻤﺷ 2 ﻧﺎﺳا ، بآ زا رﻮﺘﻧﺎﻛد ﻪﻠﻴﺳﻮﺑ ﺲ
رد هدﺎﻔﺘﺳا نﺎﻣز ﺎﺗ و ﻪﺘﺨﻳر ﮓﻧر هﺮﻴﺗ يﺎﻫ ﻪﺸﻴﺷ رد و اﺪﺟ ﺪﺷ يراﺪﻬﮕﻧ لﺎﭽﺨﻳ .
مﺎﺠﻧا ﺖﻬﺟ تﺎﺸﻳﺎﻣزآ
يزﺎﺳاﺪﺟ
تﺎﺒﻴﻛﺮﺗ شور ﻖﺒﻃ هﺪﺷ جاﺮﺨﺘﺳا ﺲﻧﺎﺳا اﺪﺘﺑا ،يرﺎﻣزر
Celiktas فاﺮﮔﻮﺗﺎﻣوﺮﻛزﺎﮔ هﺎﮕﺘﺳد ﻪﻠﻴﺳﻮﺑ ﺶﻧارﺎﻜﻤﻫ و
لﺪﻣ Hewlett–PackardHP6890 يﺎﻜﻴﻠﻴﺳ نﻮﺘﺳ ﻪﺑ ﺰﻬﺠﻣ
)DB-5 25 mm
/ 0
× 60m ﻢﻠﻴﻓ ﺖﻣﺎﺨﺿ ، 25 µm
/ 0 ( و
ﻪﻠﻌﺷ نﻮﻴﺳاﺰﻴﻧﻮﻳ عﻮﻧ زا رﻮﺘﻜﺗد )
(FID ﺪﻳدﺮﮔ راﺪﻘﻣ ﻦﻴﻴﻌﺗ .
زا
ناﺰﻴﻣ ﺎﺑ ﻞﻣﺎﺣ زﺎﮔ ناﻮﻨﻋ ﻪﺑ نژوﺮﺘﻴﻧ
୫୪
୫୧୬
9 / 0 ﺎﺗ 2 / 1 ﻪﻣﺎﻧﺮﺑ ﻖﺒﻃ
ﺪﻳدﺮﮔ هدﺎﻔﺘﺳا .
ﻪﺑ ﻪﻘﻴﻗد ﻚﻳ تﺪﻣ رد نوآ يﺎﻣد 60
ﻪﺟرد
و هﺪﻴﺳر داﺮﮕﻴﺘﻧﺎﺳ ﺖﻋﺮﺳ ﺎﺑ ﺞﻳرﺪﺗ ﻪﺑ
C
୫୧୬
4 يﺎﻣد ﻪﺑ 220
تﺪﻣ ﻪﺑ و ﺪﻴﺳر ﻪﺟرد 10
ﺲﭙﺳ ،ﺪﺷ ﻪﺘﺷاد ﻪﮕﻧ ﺎﻣد ﻦﻳا رد ﻪﻘﻴﻗد
ﺖﻋﺮﺳ ﺎﺑ
ୡ
1 ୫୧୬
ﻪﺑ 240 ﺪﻴﺳر داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد .
،ﺰﻴﻟﺎﻧآ ﺖﻬﺟ
ﻪﻧﻮﻤﻧ زا ﺮﺘﻴﻟ ﻲﻠﻴﻣ ﻚﻳ )
ﻲﻤﺠﺣ ﺖﺒﺴﻧ ﻪﺑ ناﺰﮕﻫ رد هﺪﺷ ﻞﺣ
20 (%
ﺪﻳدﺮﮔ ﻖﻳرﺰﺗ هﺎﮕﺘﺳد ﻪﺑ )
6 .(
تﺎﺒﻴﻛﺮﺗ ﻦﻴﻴﻌﺗ ﺖﻬﺟ ﺲﭙﺳ
زا هﺪﻨﻫد ﻞﻴﻜﺸﺗ لﺪﻣ ﻲﻣﺮﺟ ﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛزﺎﮔ هﺎﮕﺘﺳد
Hewlett–Packard G 1800A يﺎﻜﻴﻠﻴﺳ نﻮﺘﺳ ﻪﺑ ﺰﻬﺠﻣ
)DB-5 25mm
/ 0
× 60m ﻢﻠﻴﻓ ﺖﻣﺎﺨﺿ ، 25µm
/ 0 ( هدﺎﻔﺘﺳا
ﺪﻳدﺮﮔ . ناﺰﻴﻣ ﺎﺑ ﻞﻣﺎﺣ زﺎﮔ ناﻮﻨﻋ ﻪﺑ مﻮﻴﻠﻫزا
୫୪
7୫୧୬
/ 0 ﺪﺷ هدﺎﻔﺘﺳا
. زا ﻲﻣﺮﺟ يﺎﻫﺪﺣاو
୫
35 ﺎﺗ 425 رد 70 ev ﺪﺷ شراﺰﮔ .
ﺰﻴﻟﺎﻧآ ياﺮﺑ ﻻﺎﺑ رد هﺪﺷ ﻪﺋارا ﻪﻣﺎﻧﺮﺑ ﻖﺒﻃ ﻲﻳﺎﻣد ﻪﻣﺎﻧﺮﺑ ﺪﻳدﺮﮔ لﺎﻤﻋا ﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛزﺎﮔ .
نﺎﻣز ﻪﺴﻳﺎﻘﻣ سﺎﺳا ﺮﺑ تﺎﺒﻴﻛﺮﺗ
ﻪﻧﺎﺨﺑﺎﺘﻛ ﻲﻣﺮﺟ ﻒﻴﻃ و ﺪﻧﺎﻣ willey
ﺪﻧﺪﺷ ﻲﻳﺎﺳﺎﻨﺷ )
6 .(
ﻲﺑوﺮﻜﻴﻣ ﺪﺿ صاﻮﺧ ﻦﻴﻴﻌﺗ :
ﻲﻠﻛﺎﻴﺷﺮﺷا يﺮﺘﻛﺎﺑ ATCC 8739
نﻮﻴﺴﻜﻠﻛ يا ﻪﻘﻄﻨﻣ ﺰﻛﺮﻣ زا
يﺎﻬﻟﻮﭙﻣآ ترﻮﺻ ﻪﺑ ناﺮﻳا ﻲﺘﻌﻨﺻ يﺎﻬﻳﺮﺘﻛﺎﺑ و ﺎﻬﭼرﺎﻗ
هﺰﻴﻠﻴﻓﻮﻴﻟ ) lyophiliz (
ﺪﻳدﺮﮔ ﻪﻴﻬﺗ ، .
ﺲﭘ زا 24 ﺖﻋﺎﺳ
نﻮﻴﺳﺎﺑﻮﻜﻧا رد
يﺎﻣد 35 ﻪﺟرد
،داﺮﮕﻴﺘﻧﺎﺳ يﺮﺘﻛﺎﺑ
يﺎﻫ ﺪﺷر
ﻲﮕﻧﺎﻛ ﻚﻣ ﺖﺸﻛ ﻂﻴﺤﻣ رد ﻪﺘﻓﺎﻳ ار
رد ﻂﻳاﺮﺷ
ًﻼﻣﺎﻛ ﻞﻳﺮﺘﺳا ﺎﺑ
ﻚﻤﻛ پﻮﻟ ﻚﻳ رد ﻲﻫﺎﮕﺸﻳﺎﻣزآ يوﺎﺣ ﻞﻳﺮﺘﺳا ﻪﻟﻮﻟ
5 ﻲﻠﻴﻣ
نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ﺎﺗ هدﻮﻤﻧ طﻮﻠﺨﻣ ﻼﻣﺎﻛ ،ﻞﻳﺮﺘﺳا يژﻮﻟﻮﻳﺰﻴﻓ مﺮﺳ ﺮﺘﻴﻟ دﻮﺷ ﻞﺻﺎﺣ يﺮﺘﻛﺎﺑ زا ﻲﺘﺧاﻮﻨﻜﻳ .
تﺪﻣ ﻪﺑ ار ﻪﻟﻮﻟ ﻦﻳا 30
ﻪﻘﻴﻗد
يﺎﻣد رد 37
دراﺪﻧﺎﺘﺳا ﻪﺑﺎﺸﻣ ﻲﺗروﺪﻛ ﺎﺗ هداد راﺮﻗ ﻪﺟرد 5
/ 0
ﺪﻨﻟرﺎﻓ ﻚﻣ
௨) 108
× 5 / 1 ( ﺪﻳﺎﻤﻧ دﺎﺠﻳا )
15 ( . ﻦﻴﻴﻌﺗ ﺪﺿ صاﻮﺧ
دراﺪﻧﺎﺘﺳا سﺎﺳا ﺮﺑ و ﻚﺴﻳد رﺎﺸﺘﻧا شور ﻪﺑ ﺲﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ NCCls 2000a ﺪﻳدﺮﮔ مﺎﺠﻧا
22) .( ا ﻪﺑ يرﺎﻣزر ﺲﻧﺎﺳ
ﻞﻴﺘﻣ يد لﻼﺣ رد ار هﺪﺷ ﻚﺸﺧ ردﻮﭘ ترﻮﺻ ﺪﻳﺎﺴﻛﺎﻔﻟﻮﺳ )
(DMSO يﺎﻬﺘﻈﻠﻏ ﻪﻴﻬﺗ زا ﺲﭘ و هدﻮﻤﻧ ﻞﺣ
زا ﻲﻔﻠﺘﺨﻣ 25
ﺎﺗ µg/ml 6400 ﺮﺘﻠﻴﻓ زا رﻮﺒﻋ ﺎﺑ ،نآ زا 22
/ 0
ﺪﻳدﺮﮔ ﻞﻳﺮﺘﺳا ﺮﺘﻣوﺮﻜﻴﻣ .
،ﻲﻳﺎﻳﺮﺘﻛﺎﺑ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ زا دﻮﻫ ﺮﻳز رد
100 يوﺎﺣ يﺎﻫ ﺖﻴﻠﭘ يور ﺮﺑ ﺮﺘﻴﻟوﺮﻜﻴﻣ ﺮﻟﻮﻣ ﺖﺸﻛ ﻂﻴﺤﻣ
رﺎﮔآ نﻮﺘﻨﻴﻫ )
Muller Hinton Agar (
ﻪﻛ يرﻮﻃ ﻪﺑ ،ﻪﺘﺨﻳر
دﻮﺷ هﺪﻴﺷﻮﭘ ﺖﺧاﻮﻨﻜﻳ ﻲﺑوﺮﻜﻴﻣ ﻪﻳﻻ ﻚﻳ زا ﺖﻴﻠﭘ ﺢﻄﺳ .
ﺲﭙﺳ
يﺎﻫ ﻚﺴﻳد 6
ﺸﻏآ ماﺪﻛ ﺮﻫ ﻪﻛ يﺮﺘﻤﻴﻠﻴﻣ ﺘ
ﻪﺑ ﻪ 20 زا ﺮﺘﻴﻟوﺮﻜﻴﻣ
ﺢﻄﺳ يور ﺮﺑ ﺐﺳﺎﻨﻣ ﻞﺻاﻮﻓ ﺎﺑ ،دﻮﺑ هرﺎﺼﻋ توﺎﻔﺘﻣ يﺎﻬﺘﻈﻠﻏ ﺪﺷ هداد راﺮﻗ ﺖﻴﻠﭘ .
ﻣﺎﻛ بﺬﺟ ﺖﻬﺟ ،ﺖﻴﻠﭘ يور ﺮﺑ ﺎﻬﻜﺴﻳد ﻞ
تﺪﻣ ﻪﺑ نﻮﻴﺳﺎﺑﻮﻜﻧا زا ﻞﺒﻗ ﺎﻬﺘﻴﻠﭘ ﻲﻣﺎﻤﺗ 2
يﺎﻣد رد ﺖﻋﺎﺳ 4
زا ﺲﭘ و يراﺪﻬﮕﻧ داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد 24
رد نﻮﻴﺳﺎﺑﻮﻜﻧا ﺖﻋﺎﺳ
يﺎﻣد 37 هزاﺪﻧا ﺮﺘﻤﻴﻠﻴﻣ ﻪﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ،داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد
ﺪﻳدﺮﮔ يﺮﻴﮔ .
ناﻮﻨﻋ ﻪﺑ دراﺪﻧﺎﺘﺳا ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ يﺎﻬﻜﺴﻳد زا
و ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ يوﺎﺣ ﻚﺴﻳد زا
ﻲﻠﺻا ﻪﻧﻮﻤﻧ ناﻮﻨﻋ ﻪﺑDMSO
ﺪﺷ هدﺎﻔﺘﺳا .
ﻪﺑ ﺞﻳﺎﺘﻧ و مﺎﺠﻧا راﺮﻜﺗ رﺎﺑ ﻪﺳ ﺎﺑ ﺎﻫ ﺶﻳﺎﻣزآ ﻲﻣﺎﻤﺗ ﺪﻳدﺮﮔ شراﺰﮔ ﻦﻴﮕﻧﺎﻴﻣ ترﻮﺻ
. ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﻴﻴﻌﺗ ﻦﻴﻨﭽﻤﻫ
ﺪﺷر زا ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ ﺲﻧﺎﺳا
) : MIC Minimum
Inhibitory Concentration (
و يا ﻪﻟﻮﻟ يزﺎﺳ ﻖﻴﻗر شور ﻪﺑ
دراﺪﻧﺎﺘﺳا زا هدﺎﻔﺘﺳا ﺎﺑ NCCLS2000b
ﺪﺷ مﺎﺠﻧا ) 23 ( . ﻦﻳﺪﺑ
رد يرﺎﻣزر ردﻮﭘ ندﻮﻤﻧ ﻞﺣ ﺎﺑ ﻪﻛ ترﻮﺻ ﺖﻈﻠﻏ هد ،DMSO
ﺲﻧﺎﺳا زا ﻒﻠﺘﺨﻣ )
25 ﺎﺗ 6400 µg/ml ( ﺪﺷ ﻪﻴﻬﺗ . ﺲﭙﺳ 20
يوﺎﺣ ﻲﺑوﺮﻜﻴﻣ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ زا ﺮﺘﻴﻟ وﺮﻜﻴﻣ
௨
106
هاﺮﻤﻫ ﻪﺑ
160 زا ﻚﻳ ﺮﻫ ﻪﺑ ثاﺮﺑ نﻮﺘﻨﻴﻫ ﺮﻟﻮﻣ ﺖﺸﻛ ﻂﻴﺤﻣ زا ﺮﺘﻴﻟ وﺮﻜﻴﻣ
يوﺎﺣ يﺎﻫ ﻪﻟﻮﻟ 20
وﺮﻜﻴﻣ ﺪﻳدﺮﮔ ﻪﻓﺎﺿا ﺲﻧﺎﺳا يوﺎﺣ ﺮﺘﻴﻟ .
ﻚﻳ
ﻪﻟﻮﻟ و ﺪﻫﺎﺷ ﻪﻧﻮﻤﻧ ناﻮﻨﻋ ﻪﺑ يﺮﺘﻛﺎﺑ و ﺖﺸﻛ ﻂﻴﺤﻣ يوﺎﺣ ﻪﻟﻮﻟ و ﺖﺸﻛ ﻂﻴﺤﻣ يوﺎﺣ ﺮﮕﻳد DMSO
رد ﻲﻠﺻا ﻪﻧﻮﻤﻧ ناﻮﻨﻋ ﻪﺑ
ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ .
تﺪﻣ ﻪﺑ ﺶﻳﺎﻣزآ يﺎﻫ ﻪﻟﻮﻟ ﻲﻣﺎﻤﺗ 24
رد ﺖﻋﺎﺳ
35 ﺪﻧﺪﻳدﺮﮔ نﻮﻴﺳﺎﺑﻮﻜﻧا ﻪﺟرد .
تروﺪﻛ ﺮﻈﻧ زا ﺎﻫ ﻪﻟﻮﻟ ﺲﭙﺳ
ﺪﺷر زا ﻲﺷﺎﻧ نآ رد ﻪﻛ ﻲﺘﻈﻠﻏ ﻦﻳﺮﺘﻤﻛ و هﺪﺷ ﻲﺳرﺮﺑ يﺮﺘﻛﺎﺑ
ناﻮﻨﻋ ﻪﺑ دﻮﺑ هدﺮﻜﻧ ﺪﺷر يﺮﺘﻛﺎﺑ ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد MIC
.
ﻲﮔﺪﻨﺸﻛ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﻴﻴﻌﺗ ﺖﻬﺟ )
minimum
bactericidal concentration:MBC (
رد ﻪﻛ ﻲﻳﺎﻫ ﻪﻟﻮﻟ زا ،
، دﻮﺑ هﺪﺷ هﺪﻫﺎﺸﻣ يﺮﺘﻛﺎﺑ ﺪﺷر مﺪﻋ ﺎﻬﻧآ رد لوا ﻪﻠﺣﺮﻣ 5
وﺮﻜﻴﻣ
رد و ﻪﺘﺷادﺮﺑ ﺮﺘﻴﻟ تﺪﻣ ﻪﺑ رﺎﮔآ ﺖﻨﻳﺮﺗﻮﻧ ﺖﺸﻛ ﻂﻴﺤﻣ
24 ﺖﻋﺎﺳ
يﺎﻣد رد 35
ﺪﺷ هداد ﺖﺸﻛ ﻪﺟرد .
ﺲﻧﺎﺳا زا ﺖﻈﻠﻏ ﻦﻳﺮﺘﻤﻛ
يرﺎﻣزر ﻪﻛ يدﻮﺑﺎﻧ ﺐﺒﺳ يﺮﺘﻛﺎﺑ داﺪﻌﺗ ﻦﻳﺮﺘﺸﻴﺑ
ﺪﻳدﺮﮔ ناﻮﻨﻋ ﻪﺑ
MBC ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد .
يﺮﺘﻛﺎﺑ ﺪﺷر يور ﺮﺑ يرﺎﻣزر ﺮﺛا ﻚﻴﺘﻨﻴﺳ ﻦﻴﻴﻌﺗ :
سﺎﺳا ﺮﺑ ،يﺮﺘﻛﺎﺑ ﺪﺷر يور ﺮﺑ يرﺎﻣزر ﺮﺛا ﻚﻴﺘﻨﻴﺳ ﻲﻨﺤﻨﻣ ﻢﺳر
ﺪﺷ ﻦﻴﻴﻌﺗ دراﺪﻧﺎﺘﺳا ﻂﻳاﺮﺷ ﺖﺤﺗ ،ﺪﺷر .
زﺎﻓ رد ﺎﻬﻳﺮﺘﻛﺎﺑ زا اﺪﺘﺑا
ﻲﺑوﺮﻜﻴﻣ نﻮﻴﺴﻧﺎﭙﺳﻮﺳ ،ﻲﻤﺘﻳرﺎﮕﻟ ﺪﺷر
௨
106
ﺎﺑ 3 / 7
= PH
هدﺎﻣآ ﺪﻳدﺮﮔ ) 11 ( . ﺲﭙﺳ 50 ﺲﻧﺎﺳا ﺖﻈﻠﻏ زاﺮﺘﻴﻟوﺮﻜﻴﻣ ﻪﻛ
ﺮﺑاﺮﺑ
راﺪﻘﻣ و يﺮﺘﻛﺎﺑ يوﺎﺣ ﺶﻳﺎﻣزآ ﻪﻟﻮﻟ ﻪﺑ ،دﻮﺑMBC
5 ﻠﻴﻣ ﺮﺘﻴﻟ ﻲ
ﺖﺸﻛ ﻂﻴﺤﻣ )BHI
Brain Heart Infusion (
ﻊﻳﺎﻣ رد و ﻪﻓﺎﺿا
37 ﺖﻓﺮﮔ راﺮﻗ نﻮﻴﺳﺎﺑﻮﻜﻧا درﻮﻣ ﻪﺟرد )
10 ( .
ناﻮﻨﻋ ﻪﺑ يﺮﺘﻛﺎﺑ و ﺖﺸﻛ ﻂﻴﺤﻣ يوﺎﺣ ﻪﻟﻮﻟ ﻚﻳ ﻪﻧﻮﻤﻧ
ﻫﺎﺷ ـ رد ﺪ ﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ ـ
ﺪ . ﻬﻧﺎﻣز رد ـ ﺻ يﺎ ـ ،ﺮﻔ 5 ، 10
،
15 ، 20
، 25،30 ، 45 و 60 ﻗد ـ ﻪﻘﻴ 1 / 0 ﻴﻣ ـ ﺮﺘﻴﻟ ﻲﻠ
ﻪﻟﻮﻟ ﺮﻫ زا
،ﻪﺘﺷادﺮﺑ ﻓ ﺮﻓﺎﺑ ﺎﺑ
ــ ﻪﺑ و ﻮﺸﺘﺴﺷ ﻞﻳﺮﺘﺳا تﺎﻔﺴ
ﻣـ ﻴﻗد ﻚﻳ تﺪ ـ
ﺷ ﺎﺑ ﻪﻘ ـ
تﺪ ) 1000 RPM ( ﻔﻳﺮﺘﻧﺎﺳ ــ ژﻮﻴ
ﻓ ﺮﻓﺎﺑ رد ادﺪﺠﻣ و ﺪﻳدﺮﮔ ــ
يور ﺮﺑ و ﻞﺣ تﺎﻔﺴ BHI
ﻛ رﺎﮔآ ـ ﺪﺷ هداد ﺖﺸ .
ﭙﺳ ــ ﺲ تﺪﻣ ﻪﺑ 24
ﺖﻋﺎﺳ
رد 37 ﺪﻳدﺮﮔ نﻮﻴﺳﺎﺑﻮﻜﻧا ﻪﺟرد .
هﺪﻧز يﺎﻬﻳﺮﺘﻛﺎﺑ داﺪﻌﺗ
ﺮﻫ رد هﺪﻧﺎﻣ زا ﺲﭘ ﻲﻨﻠﻛ شرﺎﻤﺷ ﻪﻠﻴﺳﻮﺑ ،نﺎﻣز
24 ﺷ شراﺰﮔ ﻢﺘﻳرﺎﮕﻟ ترﻮﺻ ﻪﺑ و ﺪﻣآ ﺖﺳﺪﺑ ﺖﻋﺎﺳ ﺪ
) 10 ( . ﺎﻫ ﻪﺘﻓﺎﻳ
ﺎﺑ ﻪﻌﻟﺎﻄﻣ ترﻮﺻ ﻪﺘﻓﺮﮔ رد ﻦﻳا ﺶﻫوﮋﭘ زﺎﮔ ﺰﻴﻟﺎﻧآ ﺞﻳﺎﺘﻧ
يروآ ﻊﻤﺟ يرﺎﻣزر ﺲﻧﺎﺳا ﻲﻣﺮﺟ ﻲﻓاﺮﮔﻮﺗﺎﻣوﺮﻛ زا هﺪﺷ
نﺎﻫﺎﻴﮔ ﻢﻴﻠﻗا نﺎﻣﺮﻛ ﺮﻬﺷ رد هﺪﻣآ ﺖﺳﺪﺑ ﻲﻳﺎﻴﻤﻴﺷ تﺎﺒﻴﻛﺮﺗ و
لوﺪﺟ 1 دﻮﺷ ﻲﻣ هﺪﻫﺎﺸﻣ .
ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻪﺑ طﻮﺑﺮﻣ ﺞﻳﺎﺘﻧ
مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﻦﻴﻨﭽﻤﻫ و ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ رد يرﺎﻣزر ﺲﻧﺎﺳا رد ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد هﺪﺷ هدﺎﻔﺘﺳا دراﺪﻧﺎﺘﺳا يﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﺪﺷر لوﺪﺟ 2 ﺖﺳا هﺪﺷ ﺮﻛذ .
رد لوﺪﺟ 3 ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﺰﻴﻧ
ﮔﺪﻨﻨﻛ يروآ ﻊﻤﺟ يرﺎﻣزر ﺲﻧﺎﺳا ﺪﺷر زا ﻲ زا هﺪﺷ
نﺎﻫﺎﻴﮔ
ﻢﻴﻠﻗا نﺎﻣﺮﻛ ﺮﻬﺷ ﻲﻠﻛﺎﻴﺷﺮﺷا يور ﺮﺑ
ﺖﺳا هﺪﻳﺮﮔ ﺮﻛذ .
ﻪﺑ طﻮﺑﺮﻣ ﺞﻳﺎﺘﻧ و ﺲﻧﺎﺳا ﻪﻠﻴﺳﻮﺑ يﺮﺘﻛﺎﺑ يدﻮﺑﺎﻧ ﻚﻴﺘﻨﻴﺳ ﻦﻴﻴﻌﺗ
ﺖﻈﻠﻏ ﺎﺑ ﻲﻠﻛﺎﻴﺷﺮﺷا ﻪﺟاﻮﻣ µg/ml
3200 ﻪﺑ ﻪﻛ ﺲﻧﺎﺳا زا
رادﻮﻤﻧرد ،هﺪﺷ ﻞﺻﺎﺣ ﺶﻳﺎﻣزآ راﺮﻜﺗ رﺎﺑ ﻪﺳ زا ﻦﻴﮕﻧﺎﻴﻣ ترﻮﺻ 1 دﻮﺷ ﻲﻣ هﺪﻫﺎﺸﻣ .
لوﺪﺟ 1 : يروآ ﻊﻤﺟ هﺪﺷ يروآ ﻊﻤﺟ يرﺎﻣزر ﺲﻧﺎﺳا ﻲﻳﺎﻴﻤﻴﺷ ﺐﻴﻛﺮﺗ زا هﺪﺷ
نﺎﻫﺎﻴﮔ ﻢﻴﻠﻗا نﺎﻣﺮﻛ ﺮﻬﺷ
هرﺎﻤﺷ
ﺐﻴﻛﺮﺗ مﺎﻧ
ﺪﺻرد هرﺎﻤﺷ ﺐﻴﻛﺮﺗ مﺎﻧ
ﺪﺻرد
1 α-Thujene 2
/ 0 11
P-Cymenene 1
/ 0
2 -pinen
7 α / 18 12
Linalool 33
/ 12
3 Camphene
19 / 5 13
Camphonelal 3
/ 0
4 Verbenone
8 / 0 14
Camphor 9
/ 12
5 Β-Pinen
34 / 0 15
Cis-Verbenole 2
/ 0
6 Myrcene
07 / 1 16
Iso-Pinocamphone 1
/ 0
7 - 3-Carene 04
/ 0 17
Borneol 86
/ 4
8 limonene
6 / 4 18
Myrtenole 2
/ 1
9 1,8 Cineole 3
/ 21 19
Verbenone 21
/ 2
10 -Trephine 1
/ 0 20
α-Humulene 1
/ 0
لوﺪﺟ 2 : يروآ ﻊﻤﺟ يرﺎﻣزر ﺲﻧﺎﺳا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ زا هﺪﺷ
نﺎﻫﺎﻴﮔ ﻢﻴﻠﻗا نﺎﻣﺮﻛ ﺮﻬﺷ و ﻒﻠﺘﺨﻣ يﺎﻫ ﺖﻈﻠﻏ رد
ﺮﺘﻣ ﻲﻠﻴﻣ ﺐﺴﺣ ﺮﺑ دراﺪﻧﺎﺘﺳا يﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ
ﺖﻈﻠﻏ ﺲﻧﺎﺳا µg/Disc
4 8
16
32
64
128
ﻪﻟﺎﻫ ﺮﻄﻗ
1
/ 1 8± 0
5± / 11 19
/ 1 13± 07
/ 1 16± 3
/ 2 17± 66 / 1 18±
ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ
µg/Disc 30
ﻦﻴﺳﺎﻴﻣﺎﺘﻨﺟ
لﻮﻜﻴﻨﻔﻣاﺮﻠﻛ
ﻦﻴﺴﻳﺎﻣوﺮﺘﻳرا ﻦﻴﻠﻜﻳﺎﺳاﺮﺘﺗ
ﻦﻴﻠﺳ ﻲﻨﭘ ﻦﻴﺴﻳﺎﻣﻮﺘﭘﺮﺘﺳا
ﻪﻟﺎﻫ ﺮﻄﻗ
16
28
8 / 16 8
/ 27 8
/ 13 8
/ 15
لوﺪﺟ 3 : ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻪﺑ طﻮﺑﺮﻣ ﺞﻳﺎﺘﻧ ﺪﺷر زا ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ
) (MIC يروآ ﻊﻤﺟ يرﺎﻣزر ﺲﻧﺎﺳا زا هﺪﺷ
نﺎﻫﺎﻴﮔ ﻢﻴﻠﻗا
نﺎﻣﺮﻛ ﺮﻬﺷ
ﻪﻠﺣﺮﻣ
لوا ﺖﻈﻠﻏ
µg/ml 25 50 100 200 400 800 1600 3200 6400 12800
ﺪﺷر + + + + + + + - - -
ﻪﻠﺣﺮﻣ
مود ﺖﻈﻠﻏ µg/ml 1600 1800 2000 2200 2400 2600 2800 3000 3200
ﺪﺷر + + + + + + + - -
+ هﺪﺷ هﺪﻫﺎﺸﻣ يﺮﺘﻛﺎﺑ ﺪﺷر -
هﺪﺸﻧ هﺪﻫﺎﺸﻣ يﺮﺘﻛﺎﺑ ﺪﺷر –
رادﻮﻤﻧ
1 : نﺎﻣز رﺬﮔ ﺮﺛا رد يرﺎﻣزر ﺲﻧﺎﺳا ﻪﻠﻴﺳﻮﺑ ﻲﻠﻛﺎﻴﺷﺮﺷا يدﻮﺑﺎﻧ ﻚﻴﺘﻨﻴﺳ
0 1 2 3 4 5 6 7
0 5 10 15 20 25 30 45 60
Logﻩﺪﻧﺎﻣ ﻩﺪﻧﺯ ﯼﺎﻫ ﯼﺮﺘﮐﺎﺑ
ﻥﺎﻣﺯ ) ﻪﻘیﻗﺩ (
ز
ل
يﺮﻴﮔ ﻪﺠﻴﺘﻧ و ﺚﺤﺑ
يرﺎﻣزر هﺪﻨﻫد ﻞﻴﻜﺸﺗ تﺎﺒﻴﻛﺮﺗ :
،ﺎﻫﺪﻴﺋﺪﻟآ ،ﺎﻫﺮﺘﺳا زا ﻲﺒﻴﻛﺮﺗ ﺎﻬﺴﻧﺎﺳا ﺎﻫ ﻦﭘﺮﺗ و ﺎﻬﻧﻮﺘﻛ ،ﺎﻬﻠﻜﻟا
ﻲﻣ يﺪﻨﺑ ﻪﻘﺒﻃ ﻲﻋﺮﻓ و ﻲﻠﺻا تﺎﺒﻴﻛﺮﺗ هوﺮﮔ ود رد ﻪﻛ هدﻮﺑ ﺪﻧﻮﺷ ) 24 ( . دوﺪﺣ ﻲﻠﺻا تﺎﺒﻴﻛﺮﺗ 85
ﻞﻴﻜﺸﺗ ار ﺲﻧﺎﺳا ﺪﺻرد
و بآ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺲﻧﺎﺳا رد ﺎﻬﻧآ ﺖﻴﻔﻴﻛ و ﺖﻴﻤﻛ ﻪﻛ ﺪﻨﻫد ﻲﻣ ﺪﺷﺎﺑ ﺮﻴﻐﺘﻣ ﺪﻧاﻮﺗ ﻲﻣ هﺎﻴﮔ ﻦﺳو كﺎﺧ ﺐﻴﻛﺮﺗ ،اﻮﻫ )
6,25 ( . ﺎﺑ
لوﺪﺟ ﻪﺑ ﻪﺟﻮﺗ 1
،يرﺎﻣزر ﺲﻧﺎﺳا ﺰﻴﻟﺎﻧآ زا ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد ، 20
ﺪﺷ ﻲﻳﺎﺳﺎﻨﺷ ﺐﻴﻛﺮﺗ .
ﻪﻌﻟﺎﻄﻣ رد Zaouali
رد ﺶﻧارﺎﻜﻤﻫ و
ﺲﻧﻮﺗ رﻮﺸﻛ )
15 ( ،يرﺎﻣزر هﺎﻴﮔ ﺲﻧﺎﺳا ﺰﻴﻟﺎﻧآ زا ﺰﻴﻧ 25
ﺐﻴﻛﺮﺗ
ﻦﻳا كﺎﺧ ﺖﻓﺎﺑ و ﻲﻳاﻮﻫ و بآ ﻪﺑﺎﺸﺗ ﻞﻴﻟد ﻪﺑ ﻪﻛ ﺪﻣآ ﺖﺳد ﻪﺑ ﺪﺷﺎﺑ ﻲﻣ نﺎﻣﺮﻛ ﺎﺑ رﻮﺸﻛ .
،هﺎﻴﮔ نﺪﻴﭼ ﻪﻠﺣﺮﻣ و نﺎﻣز ﻦﻴﻨﭽﻤﻫ
ﻲﻣ ﺰﻴﻧ ﺮﻴﻄﻘﺗ نﺎﻣز لﻮﻃ و ﺮﻴﻄﻘﺗ شور ،هﺎﻴﮔ هدﺎﻔﺘﺳا درﻮﻣ ﺶﺨﺑ نآ ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ و ﺲﻧﺎﺳا ﺐﻴﻛﺮﺗ يورﺮﺑ ﺪﻧاﻮﺗ ﺪﺷﺎﺑ راﺬﮔﺮﻴﺛﺎﺗ )
8 ( . ﻲﺧﺮﺑ ﻪﻳﺰﺠﺗ و نداد ﺖﺳد زا لﺎﺜﻣ ناﻮﻨﻋ ﻪﺑ
تﺎﺒﻴﻛﺮﺗ ﻲﺧﺮﺑ ﻪﻳﺰﺠﺗ ،ﻲﻧﻻﻮﻃ جاﺮﺨﺘﺳا نﺎﻣز ﺖﻠﻋ ﻪﺑ راﺮﻓ
يور ﺮﺑ ﺪﻧاﻮﺗ ﻲﻣ ﺎﻣﺮﮔ ﺮﺛا ﺖﻠﻋ ﻪﺑ ﺎﻫﺮﺘﺳا ﺎﻳ عﺎﺒﺷا ﺮﻴﻏ تﺎﺒﻴﻛﺮﺗ دراﺬﮕﺑ ﺮﺛا بآ ﺎﺑ ﺮﻴﻄﻘﺗ شور زا هﺪﻣآ ﺖﺳﺪﺑ تﺎﺒﻴﻛﺮﺗ )
12,24 ( .
09 / 82 نﺎﻣﺮﻛ ﻪﻘﻄﻨﻣ رد هﺪﺷ هﺪﻴﺋور يرﺎﻣزر تﺎﺒﻴﻛﺮﺗ ﺪﺻرد
ﻞﻣﺎﺷ 1,8 Cineole ،
-pinen ،α
Camphor ،
Linalool ،
Camphene ،
Borneo ،
limonene و
Verbenone ﻲﻣ
ﻪﺑ طﻮﺑﺮﻣ ﺪﺻرد ﻦﻳﺮﺘﺸﻴﺑ ،تﺎﺒﻴﻛﺮﺗ ﻦﻳا نﺎﻴﻣ رد ﻪﻛ ﺪﺷﺎﺑ 1,8
Cineole
ﺎﺑ 3 / 21 ﺪﺷﺎﺑ ﻲﻣ ﺪﺻرد .
ﻪﻌﻟﺎﻄﻣ رد و Okoh
ﻲﺑﻮﻨﺟ يﺎﻘﻳﺮﻓآرد ﺶﻧارﺎﻜﻤﻫ )
12 ( ، Verbenone ناﻮﻨﻋ ﻪﺑ
ﺖﻠﻋ ﻪﺑ ﺪﻧاﻮﺗ ﻲﻣ ﻪﻛ ﺪﺷ ﻲﻓﺮﻌﻣ يرﺎﻣزر هﺎﻴﮔ ﻲﻠﺻا ﺐﻴﻛﺮﺗ ﺪﺷﺎﺑ هﺎﻴﮔ ﺐﻴﻛﺮﺗ يور ﺮﺑ ﻲﻳاﻮﻫ و بآ تاﺮﺛا .
تﺎﺒﻴﻛﺮﺗ ﻪﺴﻳﺎﻘﻣ ﺞﻳﺎﺘﻧ ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد هﺪﻣآ ﺖﺳﺪﺑ
Linares رد ﺶﻧارﺎﻜﻤﻫ و
ﭙﺳا ـ ﻧﺎ ـ ﺎﻴ ) 19 ( ، santoyo ﻤﻫ و
ـ ﻧارﺎﻜ ـ ﺳا رد ﺶ ـ
ﻴﻧﺎﭙ ـﺎ ) 26 ( و
Angioni ﺎﻴﻟﺎﺘﻳا رد ﺶﻧارﺎﻜﻤﻫ و
) 27 ( ﺮﺜﻛا ﺪﻫد ﻲﻣ نﺎﺸﻧ
رد ،ﺪﻧراد ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ ﻪﻛ ﺲﻧﺎﺳا هﺮﺛﻮﻣ تﺎﺒﻴﻛﺮﺗ ﺪﻧراد دﻮﺟو نﺎﻣﺮﻛ ﺮﻬﺷ زا هﺪﺷ يروآ ﻊﻤﺟ يرﺎﻣزر ﺲﻧﺎﺳا .
يرﺎﻣزر ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﺿ صاﻮﺧ :
ﻪﺑ يرﺎﻣزر ﺲﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ ﺪﺿ صاﻮﺧ ﻪﻛ يا هﺪﻤﻋ تﺎﺒﻴﻛﺮﺗ ﻞﻣﺎﺷ دﻮﺷ ﻲﻣ هداد ﺖﺒﺴﻧ ﺎﻬﻧآ 1,8 Cineole
، -pinen ،α
Camphor ،
Verbenone ﺪﺷﺎﺑ ﻲﻣ
) 14,24 ( ﻦﻳا نﺎﻴﻣ ﻪﻛ
ﺪﺘﻔﻴﺑ قﺎﻔﺗا ﺖﻟﺎﺣ ﻪﺳ ﺖﺳا ﻦﻜﻤﻣ تﺎﺒﻴﻛﺮﺗ :
يرﺎﻜﻤﻫ ﺖﻟﺎﺣ
) Synergetic (
ﻒﻟﺎﺨﻣ ﺖﻟﺎﺣ ، )
Antagonist (
ﺖﻟﺎﺣ و
هﺪﻨﻨﻛ ﺪﻳﺪﺸﺗ )
Additive .(
ﺮﺑ ،تﻻﺎﺣ ﻦﻳا زا ماﺪﻛ ﺮﻫ عﻮﻗو
ﺪﺷﺎﺑ ﻲﻣ راﺬﮔﺮﻴﺛﺎﺗ ﺲﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ يور )
1 .(
ﺰﻴﻧ ﺲﻧﺎﺳا ﻲﻋﺮﻓ تﺎﺒﻴﻛﺮﺗ ﻪﻛ دراد دﻮﺟو يدﺎﻳز ﺪﻫاﻮﺷ ﻪﭼﺮﮔا ژﺮﻨﻴﺳ تاﺮﺛا دﺎﺠﻳا ﻖﻳﺮﻃ زا لﺎﻘﺘﻧا و ﻲﻠﺻا تﺎﺒﻴﻛﺮﺗ ﻦﻴﺑ ﻲﺘﺴﻳ
ﻲﺘﻧآ صاﻮﺧ رد ﻲﻤﻬﻣ ﺶﻘﻧ يﺮﺘﻛﺎﺑ لﻮﻠﺳ نورد ﻪﺑ ﺎﻬﻧآ ﺮﺘﻧﺎﺳآ ﺪﻧراد ﺲﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ )
1,9,28 .(
ﻦﻳا رد هﺪﻣآ ﺖﺳد ﻪﺑ ﺞﻳﺎﺘﻧ
ﺪﻫد ﻲﻣ نﺎﺸﻧ ﻪﻌﻟﺎﻄﻣ 1,8 Cineole
، -pinen ﺮﻳﺎﺳ ﻪﺑ ﺖﺒﺴﻧα
يرﺎﻣزر ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﺿ ﺖﻴﺻﺎﺧ رد يﺮﺗﺮﺛﻮﻣ ﺶﻘﻧ تﺎﺒﻴﻛﺮﺗ ﻛ ﻲﻟﺎﺣ رد ﺪﻧراد ﻪ
Has-Szymanczuk رد ﺶﻧارﺎﻜﻤﻫ و
ﻪﺑ ار ﺖﻴﺻﺎﺧ ﻦﻳا ،ﺪﻨﻠﻫ Verbenone
و 1,8 Cineole ﺖﺒﺴﻧ
ﺪﻧداد ) 29 .(
صاﻮﺧ ﻪﻛ ﺪﺷﺎﺑ ﻲﻣ ﺐﻠﻄﻣ ﻦﻳا ﺪﻳﻮﻣ عﻮﺿﻮﻣ ﻦﻳا
ﻖﻃﺎﻨﻣ رد ﺲﻧﺎﺳا ﺮﺛﻮﻣ ﺐﻴﻛﺮﺗ و يرﺎﻣزر ﻲﺑوﺮﻜﻴﻣ ﻲﺘﻧآ زا ﻲﺷﺎﻧ و ﻪﺘﺷاد يدﺎﻳز عﻮﻨﺗ ،ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ رد و نﻮﮔﺎﻧﻮﮔ ﻲﻳﺎﻴﻤﻴﺷ ﺐﻴﻛﺮﺗ توﺎﻔﺗ ﻂﻴﺤﻣ و ﻂﻳاﺮﺷ ،ﻪﻘﻄﻨﻣ ﺮﻫ رد ﺲﻧﺎﺳا
جاﺮﺨﺘﺳا يﺎﻬﺷور ،ﺖﺷادﺮﺑ نﺎﻣز ،هﺎﻴﮔ تﺎﺼﺨﺸﻣ ،ﺖﺸﻛ
ﺪﺷﺎﺑ ﻲﻣ هدﺎﻔﺘﺳا درﻮﻣ ﺲﻧﺎﺳا ﺖﻈﻠﻏ و ﺲﻧﺎﺳا )
15,29,30 (
. ﻲﻜﻳ
ﻢﻬﻣ يﺎﻬﺷور زا هدﺎﻔﺘﺳا ،ﺎﻬﺴﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺮﺛا ﻦﻴﻴﻌﺗ ﺖﻬﺟ
ﻪﺑ ﺲﻧﺎﺳا ،نآ رد ﻪﻛ ﺪﺷﺎﺑ ﻲﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ شور زا ﺪﻫد ﻲﻤﻧ ار دﻮﺧ فاﺮﻃا رد ﺪﺷر هزﺎﺟا ﺎﻫ يﺮﺘﻛﺎﺑ .
ﺶﻳاﺰﻓا ﺎﺑ
نآ فاﺮﻃا رد ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ،ﻚﺴﻳد ﺮﻫ رد ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﻪﻛ ددﺮﮔ ﻲﻣ ﺮﺗ يﻮﻗ ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ و هﺪﺷ ﺮﺘﺸﻴﺑ ﺰﻴﻧ نﺎﺸﻧ و ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ،ﺲﻧﺎﺳا ﺖﻈﻠﻏ ﻢﻴﻘﺘﺴﻣ طﺎﺒﺗرا هﺪﻨﻫد
ﺪﺷﺎﺑ ﻲﻣ ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ .
لوﺪﺟ رد ﻪﻛ رﻮﻄﻧﺎﻤﻫ 2
ﺖﻈﻠﻏ رد ،دﻮﺷ ﻲﻣ هﺪﻫﺎﺸﻣ µg/Disc
32 ﺮﻄﻗ ،يرﺎﻣزر ﺲﻧﺎﺳا
ﺮﺑاﺮﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ 07
/ 1 16± ﺮﺑاﺮﺑ ﻪﻛ هﺪﻳدﺮﮔ ﻞﺻﺎﺣ ﺮﺘﻣ ﻲﻠﻴﻣ
ﻂﺳﻮﺗ هﺪﻣآ ﺖﺳﺪﺑ ﺮﻄﻗ زا ﺮﺘﻤﻛ ﺎﻳ يﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ
ﺎﺘﻨﺟ ﺪﺷﺎﺑ ﻲﻣ ﻦﻴﺴﻳﺎﻣوﺮﺘﻳرا و ﻦﻴﺴﻳﺎﻣﻮﺘﭘﺮﺘﺳا ،ﻦﻴﻠﻴﺳ ﻲﻨﭘ ،ﻦﻴﺴﻳﺎﻣ .
رد ﺪﻧاﻮﺗ ﻲﻣ نﺎﻣﺮﻛ ﻢﻴﻠﻗا يرﺎﻣزر ﺲﻧﺎﺳا ﻪﻛ ﺖﺳﺎﻨﻌﻣ ناﺪﺑ ﻦﻳا ﻲﺘﻧآ ﻦﻳا ﻪﺑ ﺖﺒﺴﻧ يﺮﺘﺸﻴﺑ ﺎﻳ و نﺎﺴﻜﻳ ترﺪﻗ ،هﺪﺷ دﺎﻳ ﺖﻈﻠﻏ ﺎﺑ ﻲﻨﻳﺰﮕﻳﺎﺟ ﻞﺑﺎﻗ و ﺪﻫد نﺎﺸﻧ ﻲﻠﻛﺎﻴﺷﺮﺷا ﺮﺑاﺮﺑ رد ﺎﻫ ﻚﻴﺗﻮﻴﺑ ﺪﺷﺎﺑ ﻲﻣ ﺎﻬﻧآ .
ﻪﻌﻟﺎﻄﻣ ﻲﻃ Gachkar
رد ﺶﻧارﺎﻜﻤﻫ و
ناﺮﻳا ) 10 ( ﺮﺑاﺮﺑ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﺮﻄﻗ ﺰﻴﻧ 67
/ 16 ﺪﻣآ ﺖﺳﺪﺑ ﺮﺘﻤﻴﻠﻴﻣ
دراد ﺖﻘﺑﺎﻄﻣ ﻪﻌﻟﺎﻄﻣ ﻦﻳا ﺞﻳﺎﺘﻧ ﺎﺑ ﻪﻛ .
ﻦﻳا ﺞﻳﺎﺘﻧ ﻪﺴﻳﺎﻘﻣ ﻦﻴﻨﭽﻤﻫ
ﺞﻳﺎﺘﻧ ﺎﺑ ﻪﻌﻟﺎﻄﻣ Bosnic
ﺶﻧارﺎﻜﻤﻫ و ﻲﻨﺳﻮﺑ رﻮﺸﻛ رد
) 31 ( ﻪﻛ
ﺮﻄﻗ 13 يرﺎﻣزر ﺲﻧﺎﺳا ﺪﻫد ﻲﻣ نﺎﺸﻧ ﺪﻧدروآ ﺖﺳﺪﺑ ار ﺮﺘﻤﻴﻠﻴﻣ ﻲﺑوﺮﻜﻴﻣ ﺪﺿ ﺖﻴﺻﺎﺧ ﺮﺑاﺮﺑ ﺖﻈﻠﻏ رد ،نﺎﻣﺮﻛ ﻢﻴﻠﻗا ﻪﺑ ﻖﻠﻌﺘﻣ
دراد يﺮﺗ يﻮﻗ .
ﺪﺿ ﺖﻴﺻﺎﺧ ﻦﻴﻴﻌﺗ ﺖﻬﺟ ﻢﻬﻣ يﺎﻬﺷور ﺮﮕﻳد زا
و ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦﻴﻴﻌﺗ ،ﺎﻬﺴﻧﺎﺳا ﻲﺑوﺮﻜﻴﻣ ﺪﺷﺎﺑ ﻲﻣ ﺲﻧﺎﺳا ﻲﮔﺪﻨﺸﻛ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ .
ﺎﺳا دﺮﻜﻠﻤﻋ ﺮﺑ ﺲﻧ
ﺪﺷﺎﺑ ﻖﻳﺮﻃ ود ﻪﺑ ﺖﺳا ﻦﻜﻤﻣ يﺮﺘﻛﺎﺑ يور :
يﺮﺘﻛﺎﺑ ﺪﺷر زا ﻂﻘﻓ
ﺪﻳﺎﻤﻧ يﺮﻴﮔﻮﻠﺟ )
Bacteriostatic (
يﺎﻬﻟﻮﻠﺳ زا يداﺪﻌﺗ ﺎﻳ و دﺮﺒﺑ ﻦﻴﺑ زا ار يﺮﺘﻛﺎﺑ
) Bactericide (
ﻒﻗﻮﺘﻣ ﺖﻴﺻﺎﺧ ﻪﻛ
ﺪﻧاﻮﺗ ﻲﻣ يﺮﺘﻛﺎﺑ لﻮﻠﺳ و هدﻮﺑ ﺖﺸﮔﺮﺑ ﻞﺑﺎﻗ ﺲﻧﺎﺳا ﻲﮔﺪﻨﻨﻛ ﺪﻳﺎﻤﻧ يزﺎﺳزﺎﺑ ار دﻮﺧ ادﺪﺠﻣ .
فﻼﺧﺮﺑ ﻪﻛ ﻲﮔﺪﻨﺸﻛ ﺖﻴﺻﺎﺧ
دﻮﺷ ﻲﻣ دﻮﺑﺎﻧ ﻲﻠﻛ رﻮﻄﺑ لﻮﻠﺳ )
32 ( . ﻞﻗاﺪﺣ ،ﻪﻌﻟﺎﻄﻣ ﻦﻳا رد
ﺮﺑاﺮﺑ ﻲﻠﻛﺎﻴﺷﺮﺷا يﺮﺘﻛﺎﺑ ﺪﺷر زا ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ µg/ml 3000 ) لوﺪﺟ 3 ( يﺮﺘﻛﺎﺑ ﻲﮔﺪﻨﺸﻛ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و
ﻲﻠﻛﺎﻴﺷﺮﺷا µg/ml
3200 ﺪﻣآ ﺖﺳد ﻪﺑ .
نﺎﻴﻣ رد ﻪﻜﻨﻳا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ
ﺪﺷر زا ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ،ﺎﻬﻜﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﺮﺑاﺮﺑ دراﺪﻧﺎﺘﺳا رﻮﻃ ﻪﺑ ﻲﻠﻛﺎﻴﺷﺮﺷا ياﺮﺑ ﻦﻴﺴﻳﺎﻣﺎﺘﻨﺟ µg/ml
6250 ﺪﺷﺎﺑ ﻲﻣ
، ﺖﻈﻠﻏ رد ﺪﻧاﻮﺗ ﻲﻣ يرﺎﻣزر ﺲﻧﺎﺳا ﻦﻳاﺮﺑﺎﻨﺑ
ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﻦﻳا ياﺮﺑ ﻲﺒﺳﺎﻨﻣ ﻲﻌﻴﺒﻃ ﻦﻳﺰﮕﻳﺎﺟ ،يﺮﺘﻤﻛ رﺎﻴﺴﺑ ﺪﺷﺎﺑ . Celiktas ﻪﻴﻛﺮﺗ رد ﺶﻧارﺎﻜﻤﻫ و
) 6 ( ﺖﻈﻠﻏ ﻞﻗاﺪﺣ
ﺮﺑاﺮﺑ ار ﻲﻠﻛﺎﻴﺷﺮﺷا يﺮﺘﻛﺎﺑ ﺪﺷر زا ﻲﮔﺪﻨﻨﻛ ﺖﻌﻧﺎﻤﻣ µg/ml
10000 ، Pintore ﺶﻧارﺎﻜﻤﻫ و
ﺎﻴﻟﺎﺘﻳا رد ) 11 ( µg/ml 4000 و
Zizovic ﺶﻧارﺎﻜﻤﻫ و
) 33 ( 3200 ﺎﺗ µg/ml 12800 ياﺮﺑ
رﺎﻣزر ﻒﻠﺘﺨﻣ يﺎﻫ ﻪﻧﻮﮔ ﺪﻧا هدﻮﻤﻧ شراﺰﮔ ي
.
ﻲﻠﻛﺎﻴﺷﺮﺷا يدﻮﺑﺎﻧ ﻚﻴﺘﻨﻴﺳ :
ﺮﺑاﺮﺑ رد نﺎﺸﺘﻈﻓﺎﺤﻣ ﺖﻬﺟ ﻪﻳﻮﻧﺎﺛ يﺎﻫ ﺖﻴﻟﻮﺑﺎﺘﻣ ،نﺎﻫﺎﻴﮔ ﺮﺜﻛا رد ﺪﻳآ ﻲﻣ دﻮﺟو ﻪﺑ ﺎﻬﭼرﺎﻗ و ﺎﻬﻳﺮﺘﻛﺎﺑ ،ﺎﻬﺳوﺮﻳو )
34 ( ﻖﻳﺮﻃ زا ﻪﻛ
ﻣ ﻒﻗﻮﺘﻣ ار ﺎﻫ ﻢﺴﻴﻧﺎﮔراوﺮﻜﻴﻣ ﺖﻴﻟﺎﻌﻓ ﻢﺴﻴﻧﺎﻜﻣ ﺪﻨﭼ ﺪﻧزﺎﺳ ﻲ
.
هدﻮﺑ ﺲﻧﺎﺳا رد دﻮﺟﻮﻣ ﻲﻠﻨﻓ تﺎﺒﻴﻛﺮﺗ ﻪﻠﻴﺳﻮﺑ ﻢﺴﻴﻧﺎﻜﻣ ﻦﻳﺮﺘﻤﻬﻣ زا دزﺎﺳ ﻲﻣ ردﺎﻗ ار ﺎﻬﻧآ يﺰﻳﺮﮔ بآ ﺖﻴﺻﺎﺧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻪﻛ ﺎﻬﻨﻴﺌﺗوﺮﭘ ﻦﻴﻣآ ﻞﻴﺴﻛورﺪﻴﻫ و ﻦﻴﻣآ يﺎﻬﻫوﺮﮔ ﻪﺑ لﺎﺼﺗا ﻖﻳﺮﻃ و يرﺪﻨﻛﻮﺘﻴﻣ و ﻲﻟﻮﻠﺳ ءﺎﺸﻏ يﺎﻫﺪﻴﭙﻴﻟ ﻪﻳﺰﺠﺗرد يﺮﺛﻮﻣ ﺶﻘﻧ ﻪﺠﻴﺘﻧرد و ءﺎﺸﻏ يﺮﻳﺬﭘذﻮﻔﻧ ﺮﻴﻴﻐﺗ ﻪﺘﺷاد يﺮﺘﻛﺎﺑ لﻮﻠﺳ گﺮﻣ
ﺪﻨﺷﺎﺑ ) 9,31 .(
ﻞﻗاﺪﺣ ﺎﺑ ﻲﻠﻛﺎﻴﺷﺮﺷا يﺮﺘﻛﺎﺑ ﻪﻬﺟاﻮﻣ زا ﺲﭘ
يﺮﺘﻛﺎﺑ يدﻮﺑﺎﻧ ﺖﻬﺟ يرﺎﻣزر ﺲﻧﺎﺳا ﺖﻈﻠﻏ )
(MBC داﺪﻌﺗ ،
رد ﻪﻛ ﺪﻳدﺮﮔ ﺖﺒﺛ ﻒﻠﺘﺨﻣ يﺎﻬﻧﺎﻣز رد هﺪﻧﺎﻣ هﺪﻧز يﺎﻬﻳﺮﺘﻛﺎﺑ رادﻮﻤﻧ 1 دﻮﺷ ﻲﻣ هﺪﻫﺎﺸﻣ .
يرﺎﻣزر ﺲﻧﺎﺳا ،رادﻮﻤﻧ ﻦﻳا سﺎﺳا ﺮﺑ
زا ﺲﭘ ﺪﻧاﻮﺗ ﻲﻣ 25
ﻘﻴﻗد ﻦﻴﺑ زا ار ﻲﻠﻛﺎﻴﺷﺮﺷا يﺎﻬﻳﺮﺘﻛﺎﺑ ﻲﻣﺎﻤﺗ ﻪ
دﺮﺒﺑ . ﺞﻳﺎﺘﻧ ﺎﺑ ﻪﺠﻴﺘﻧ ﻦﻳا Gachkar
ناﺮﻳا رد ﺶﻧارﺎﻜﻤﻫ و
دراد ﺖﻘﺑﺎﻄﻣ )
10 .(
ﻪﻛ يا ﻪﻌﻟﺎﻄﻣ رد ﻦﻴﻨﭽﻤﻫ Pintore
و
يور يرﺎﻣزر ﺲﻧﺎﺳا زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻴﻟﺎﺘﻳا رد ﺶﻧارﺎﻜﻤﻫ نﺎﻣز ﻪﺑ ﺪﻧداد مﺎﺠﻧا سرﻮﺋآ كﻮﻛﻮﻠﻴﻓﺎﺘﺳا 30
ﺖﻬﺟ ﻪﻘﻴﻗد
يدﻮﺑﺎﻧ 80 ﺪﻨﺘﻓﺎﻳ ﺖﺳد يﺮﺘﻛﺎﺑ ﺪﺻرد )
11 (.
ﺎﺑ ﻪﻌﻟﺎﻄﻣ ، هﺪﺷ مﺎﺠﻧا طﺎﻘﻧ ﺮﻳﺎﺳ رد ﻪﻛ يدﺪﻌﺘﻣ تﺎﻌﻟﺎﻄﻣ ﻪﺑ ﻪﺟﻮﺗ نﺎﻣﺮﻛ رد يرﺎﻣزر ﺲﻧﺎﺳا ﻲﻳﺎﻴﻤﻴﺷ ﺐﻴﻛﺮﺗ رﺎﺑ ﻦﻴﻟوا ياﺮﺑ ﺮﺿﺎﺣ
ﺖﺳا هداد راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ ار .
ﺎﺑ ﻪﻛ ﺪﻫد ﻲﻣ نﺎﺸﻧ ﻞﺻﺎﺣ ﺞﻳﺎﺘﻧ
ﻪﻘﻄﻨﻣ رد نﺎﻣﺮﻛ ﻦﺘﻓﺮﮔ راﺮﻗ ﻪﺑ ﻪﺟﻮﺗ ﻲﻳاﻮﻫ و بآ
ﻚﺸﺧ ﻪﻤﻴﻧ و
لﺪﺘﻌﻣ ﻲﻣﺎﻤﺗ ﻪﻘﻄﻨﻣ ﻦﻳا رد يرﺎﻣزر هﺎﻴﮔ زا هﺪﻣآ ﺖﺳﺪﺑ ﺲﻧﺎﺳا ، ﻪﺑ ﺖﺒﺴﻧ و ﻪﺘﺷاد دﻮﺧ رد ﻲﺗﻻﺎﺑ ﺪﺻرد ﺎﺑ ار ﺮﺛﻮﻣ تﺎﺒﻴﻛﺮﺗ
رﺎﻴﺴﺑ ﻲﻳﺎﻳﺮﺘﻛﺎﺑ ﺪﺿ ﺖﻴﺻﺎﺧ ،طﺎﻘﻧ ﺮﻳﺎﺳ رد هﺪﺷ مﺎﺠﻧا تﺎﻌﻟﺎﻄﻣ ﻪﻛ ﺎﺠﻧآ زا و ﺪﻫد ﻲﻣ نﺎﺸﻧ دﻮﺧ زا ﻦﻴﻳﺎﭘ يﺎﻬﺘﻈﻠﻏ رد ﻲﺑﻮﺧ ﺮﻳدﺎﻘﻣرد يﺎﻫ ﻚﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﻲﺧﺮﺑ ﻪﺑ ﺖﺒﺴﻧ يﺮﺘﻤﻛ رﺎﻴﺴﺑ
ﺖﻴﻠﺑﺎﻗ ﻦﻳاﺮﺑﺎﻨﺑ ،دﺮﺒﺑ ﻦﻴﺑ زا ار ﻲﻠﻛﺎﻴﺷﺮﺷا ﺪﻧاﻮﺗ ﻲﻣ ﻲﻳوراد ﺪﺷﺎﺑ ﻲﻣ اراد ار ﺎﻬﻜﻴﺗﻮﻴﺑ ﻲﺘﻧآ ﻦﻳا ﺎﺑ ﻲﻨﻳﺰﮕﻳﺎﺟ .
ﻲﻧادرﺪﻗ و ﺮﻜﺸﺗ
ﺖﺷاﺪﻬﺑ تﺎﻘﻴﻘﺤﺗ ﺰﻛﺮﻣ رد ﻲﺗﺎﻘﻴﻘﺤﺗ حﺮﻃ ﺐﻟﺎﻗ رد ﻪﻌﻟﺎﻄﻣ ﻦﻳا ﺎﺑ و نﺎﻣﺮﻛ ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻂﻴﺤﻣ ﺖﻧوﺎﻌﻣ ﻲﻟﺎﻣ ﺖﻳﺎﻤﺣ
ﺖﺳا هﺪﺷ مﺎﺠﻧا هﺎﮕﺸﻧاد ﻦﻳا يروآ ﻦﻓ و تﺎﻘﻴﻘﺤﺗ .
زا ﻦﻴﻘﻘﺤﻣ
ﻢﻧﺎﺧ رﺎﻛﺮﺳ و يﺪﻬﺸﻣ ﺲﮔﺮﻧ سﺪﻨﻬﻣ ﻢﻧﺎﺧ رﺎﻛﺮﺳ يرﺎﻜﻤﻫ ﻂﻴﺤﻣ ﺖﺷاﺪﻬﺑ ﻲﺳﺪﻨﻬﻣ نﺎﺳﺎﻨﺷرﺎﻛ يﺮﻫﺎﻃ ﻢﻳﺮﻣ سﺪﻨﻬﻣ ﻲﻣ ﺮﻜﺸﺗ و ﻲﻧادرﺪﻗ ﺪﻨﻳﺎﻤﻧ
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Essential oils on Escherichia Coli and Its Kinetic
Malakootian M (PhD)1 Hatami B (MSc)2
1.Corresponding Author:Professor Environmental Health Research Center and Department of Environmental Health , Kerman University of Medical Sciences, Kerman, Iran
2.MSc in Environmental Health Engineering, Kerman University of Medical Sciences, Kerman, Iran
Abstract
Background: due to the resistance of microorganisms against antibiotics, using medicinal plants to achieve the new compounds and overcome them is essential. In this study the effective compounds and antibacterial effects of rosemary essential oil on Escherichia coli, as one of the most common pathogenic bacteria was investigated and its kinetics was determined.
Methods: After collecting plant from grown plants in Kerman's climate and drying it, the extraction of essential oil was done by hydrodistillation method. GC analysis was performed for Essential oil and compounds were identified. The antimicrobial activities were tested by the disc diffusion technique and were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC). The kinetic of the essential oil on bacteria was determined.
Results: Twenty compounds were identified in the rosemary essential oils, 82.09% of which contained 1,8 Cineole, Borneo, α-pinen, Camphor, Linalool, Camphene, limonene and Verbenone.
The diameters of the inhibition zones obtained by 32 µg/Disc concentration of essential oil, was more than diameters of the inhibition zones of antibiotics gentamicin, penicillin, streptomycin and erythromycin. The MIC and MBC in the study were obtained as 3000 ஜ
୫୪ and 3200 ஜ
୫୪ respectively.
Complete death time for destruction of E. coli was also 25 minutes.
Conclusion: Rosemary essential oil collected from Kerman with some pharmaceutical antibiotics revealed to have very good antibacterial properties against Escherichia coli.
Keywords: Rosmarinus officinalis, Essential oil, Escherichia coli, Microorganism, Antibacterial
