ةدﺎﻀﻤﻟا مﺎﺴﺟﻷا سﺎﻴﻘﻟ ﺔﻴﺟﻮﻟوﺮﻴﺳ قﺮﻃ ﺮﻳﻮﻄﺗ لﺎﻔﻃﻷا ىﺪﻟ لﺎﻔﻃﻷا ﻞﻠﺷ سوﺮﻴﻔﻟ
ﺐﻟﺎﻄﻟا
بﺎﻬﻳإ ﻦﺑ
ﺮﻄﻣ ﻦﻴﺴﺣ
ﺺﻠﺨﺘﺴﻤﻟا
لﺎ ﻔﻃﻷا ﻞﻠ ﺷ تﺎ ﺳوﺮﻴﻔﺑ ﺔﺑﺎ ﺻﻺﻟ ﺎ ﻳﻼﺨﻟا عاﻮ ﻧأ ﺾ ﻌﺑ ﺔ ﻴﻠﺑﺎﻗ ﻰ ﻠﻋ فﺮ ﻌﺘﻟا ﻢ ﺗ ﺔ ﺳارﺪﻟا ﻩﺬ ه ﻲ ﻓ
ﻲ ﺤﻟا حﺎ ﻘﻠﻟا ﻲ ﻓ مﺪﺨﺘ ﺴﻤﻟا .
نﺎﻃﺮ ﺳ ﺎ ﻳﻼﺧ ﻞ ﺜﻣ نﺎ ﺴﻧﻻا ﺎهرﺪ ﺼﻣ ﻪﻣﺪﺨﺘ ﺴﻤﻟا ﺎ ﻳﻼﺨﻟا ﺾ ﻌﺑو
تﻼ ﻀﻌﻟا (RD)
نﺎ ﺴﻧﻻا ﺪ ﻠﺟ ﺎ ﻳﻼﺧو (HSF)
ىﺮ ﺧأ ﺎ ﻳﻼﺧو
ﺔ ﻟﻮﺤﺘﻣ ﻪ ﻴﻣدﺁ (Hep2)
ﺎ ﻳﻼﺧو
ﺳ ﻪ ﺋﺮﻟا نﺎﻃﺮ (A549)
دﺮ ﻘﻟا ﺔ ﻴﻠآ ﺎ ﻳﻼﺧ ﻞ ﺜﻣ ناﻮ ﻴﺤﻟا ﺎهرﺪ ﺼﻣ ﺎ ﻳﻼﺨﻟا ﻦ ﻣ ىﺮ ﺧأ عاﻮ ﻧأو
ﺮﻀﺧﻷا ﻲﻘﻳﺮﻓﻻا (Vero)
بﻼﻜﻟا ﺔﻴﻠآ ﺎﻳﻼﺧو .(MDCK)
ةرﺪ ﻗ ﻰ ﻠﻋ فﺮ ﻌﺘﻠﻟ ﺔﻟوﺎﺤﻣ ﻲﻓ ﻚﻟذو
وأ نﺎ ﺴﻧﻹا ﻢ ﺴﺟ ﻲ ﻓ ﺔ ﻔﻠﺘﺨﻣ ءﺎ ﻀﻋأ ﺎ ﻳﻼﺧ ﺔﺑﺎﺻإ ﻰﻠﻋ حﺎﻘﻠﻟا ﻲﻓ مﺪﺨﺘﺴﻤﻟا لﺎﻔﻃﻷا ﻞﻠﺷ سوﺮﻴﻓ ناﻮﻴﺤﻟا .
ﺺﺤﻔﻟﺎﺑو ضاﺮ ﻋﻷا ﺎ ﻬﻴﻠﻋ تﺮ ﻬﻇ ﻪﻣﺪﺨﺘ ﺴﻤﻟا ﺎ ﻳﻼﺨﻟا ﻦ ﻣ ﻂ ﻘﻓ ﻦﻴﻋﻮ ﻧ نأ ﻦﻴ ﺒﺗ ﻲﺑﻮﻜ ﺳوﺮﻜﻴﻤﻟا
ﺔﻴﺿﺮﻤﻟا (CPE)
ﺎ ﻤﻣ ﺎ ﻳﻼﺨﻟا ﻲﻗﺎ ﺑ ﻰ ﻠﻋ ﺔﻴﺿﺮﻤﻟا ضاﺮﻋﻷا ﺮﻬﻈﺗ ﻢﻟو سوﺮﻴﻔﻟﺎﺑ ﺔﺑﺎﺻﻹا ﺔﺠﻴﺘﻧ
سوﺮﻴﻔﻟﺎ ﺑ ﺔ ﺻﺎﺨﻟا تﻼﺒﻘﺘ ﺴﻤﻟا ﺮﻓﻮ ﺗ ىﺪ ﻣ ﻮ ه سوﺮﻴﻔﻟﺎﺑ ﺔﻴﻠﺨﻟا ﺔﺑﺎﺻﻹ ﻲﺴﻴﺋﺮﻟا ﻞﻣﺎﻌﻟا نأ ﺪآﺆﻳ ﺔﻴﻠﺨﻟا ﺢﻄﺳ ﻰﻠﻋ .
او
دﺪ ﻋ ﻊ ﻤﺠﺑ ﺔ ﺳارﺪﻟا ﺖﻠﻤﻜﺘ ﺳ )
٢٠٠ ( ﻰ ﻟا ﺮﻬ ﺷ ﻦﻴﺑﺎ ﻣ ﻢهرﺎ ﻤﻋأ حواﺮ ﺘﻳ لﺎ ﻔﻃأ ﻦ ﻣ مد ﺔ ﻨﻴﻋ
٤ ﻦﻴﺑﺎﻣ ﻢهرﺎﻤﻋأ حواﺮﺘﺗ لﺎﻔﻃأ ﻦﻣو تاﻮﻨﺳ ٥
ﻰﻟا تاﻮﻨﺳ ١٥
ﺔ ﻳﺎﻤﺤﻟا ىﺪ ﻣ ﺔﺳارﺪﻟ ﻚﻟذو تاﻮﻨﺳ
ﻢﻬﻨﻴﺼﺤﺗ ﺔﺠﻴﺘﻧ لﺎﻔﻃﻷا ءﻻﺆه ىﺪﻟ ةﺮﻓﻮﺘﻤﻟا ﺔﻴﻋﺎﻨﻤﻟا برﺎﺠﺘﻟا ﺖﻳﺮﺟأو ﻲﺤﻟا لﺎﻔﻃﻷا ﻞﻠﺷ حﺎﻘﻠﺑ
ﻰ ﺘﺣ ﺮﻬ ﺷ ﺮ ﻤﻋ ﻦ ﻣ لﺎ ﻔﻃﻷا نأ ﺪ ﺟو ﺪ ﻗو ةدﺎ ﻀﻤﻟا مﺎ ﺴﺟﻷا ﻦ ﻋ ﻒ ﺸﻜﻠﻟ ﺔﻴﺟﻮﻟوﺮﻴ ﺴﻟا ٣
رﻮﻬ ﺷ
سوﺮﻴﻔﻟﺎ ﺑ ﺔﺑﺎ ﺻﻹا ﻦ ﻣ ﺔ ﻳﺎﻤﺤﻟا ﻢ ﻬﻟ تﺮ ﻓو مﻷا ﻦ ﻣ ﻪﺒ ﺴﺘﻜﻣ ﺔ ﻋﺎﻨﻣ ﻢﻬﻳﺪ ﻟ .
ﺔ ﺟرد ﺖ ﻋﻮﻨﺗ ﺪ ﻗو
ﻜ ﺸﺑ ﺎ ﻬﻨﻜﻟو ﺔﻔﻠﺘﺨﻤﻟا رﺎﻤﻋﻷا ﻲﻓ لﺎﻔﻃﻷا ﻦﻴﺑ ﺔﻴﻋﺎﻨﻤﻟا ﺔﺑﺎﺠﺘﺳﻻا
ﻓﻮ ﺗ ﻻو ﺔﻔﻴﻌ ﺿ مﺎ ﻋ ﻞ ﺮ
ﺔ ﻳﺎﻤﺤﻟا
ﺎ ﻨﻟ ﻰﻨ ﺴﺘﻳ ﻰ ﺘﺣ لﺎ ﻔﻃﻷا ﻦ ﻣ ﺮ ﺒآأ داﺪ ﻋأ ﻰ ﻠﻋ ﺔ ﺳارﺪﻟا ﻩﺬ ه ﻦ ﻣ ﺪ ﻳﺰﻤﻟا ﻰ ﻟا ﺎﻨﻌﻓﺪ ﻳ ﺎ ﻤﻣ ﺔﺑﻮﻠﻄﻤﻟا ﺔﻜﻠﻤﻤﻟا ﻲﻓ لﺎﻔﻃﻷا ﻞﻠﺷ ﺪﺿ ﻦﻴﺼﺤﺘﻟا ﺞﻣﺎﻧﺮﺑ ﺔﻌﺟاﺮﻣ .
لﺎ ﻔﻃﻷا ﻲﻓ ﻩراﺮﻜﺗو ﻦﻴﺼﺤﺘﻟا ةدﺎﻋإ ةروﺮﺿ ﻮﺤﻧ ﺎﻧرﺎﻈﻧأ ﺖﺘﻔﻟ ﺪﻗ ﺔﺳارﺪﻟا ﻩﺬه نﺎﻓ مﺎﻋ ﻞﻜﺸﺑو أ ﺚﻴﺣ لﺪ ﻌﻣ عﺎ ﻔﺗرا ﻰ ﻟا يدﺆ ﻳ ﺪﻗ ﻦﻴﺼﺤﺘﻟا راﺮﻜﺗ ﻦﻜﻟو اﺮﻴﺜآ ﻒﻠﺘﺨﺗ ﻢﻬﻳﺪﻟ ﺔﻴﻋﺎﻨﻤﻟا ﺔﺑﺎﺠﺘﺳﻻا ن
ﺔﻣزﻼﻟا ﺔﻳﺎﻤﺤﻟا ﺮﻓﻮﻳ ﺎﻤﻣ ﺔﻴﻋﺎﻨﻤﻟا ةدﺎﻀﻤﻟا مﺎﺴﺟﻷا .
Development of a serological technique for the titration of antibodies for polioviruses in
children
Ehab Husain Mattar Abstract
Poliomyelitis (polio) is a highly infectious disease caused by a virus. It invades the nervous system, and can cause total paralysis in a matter of hours. It can strike at any age, but affects mainly children under three (over 50% of all cases). The virus enters the body through the mouth and multiplies in the intestine.
In the present study host cell susceptibility to infection with attenuated polioviruses strains was reviewed with 6 types of cell lines, Vero, RD, Hep2, A549, MDCK and HSF. Infectivity was measured by staining with trypan blue dye and microscopic examination. The results revealed that Vero and RD cells were highly susceptible for attenuated poliovirus infection. No cytopathic effects were observed on other cell types during the incubation period of ten days. Immune response against oral
poliovirus vaccine was also measured between vaccinated children and young adults between the age of one month to fifteen years. It was noticed that children of 1-2 months of age gained a protective immunity against polioviruses from their mothers.
The responsiveness against poliovirus vaccine was varied much between children at different ages. It was noticed that some of the studied children have no protective levels of antibodies which represents a high risk factor if they are exposed to wild polioviruses.
