Nghien ciru - Ky thuat
J Nghien ciru dae diem hinh thai va dae diem vi hoe eua eay ha thu 6 do (Fallopia multiflora (Thunb.) Haralds.)
Nguyen Van Bach^ Hoang Viet Dung**, Do Quyen', Pham Tuan Anh^
'Hoc vien Quan y. 'Tnrong Dgi hoc Duf/c Hd Npi 'E-mail: vietdungk85@yahoo. com S u m m a r y
The morphological characters of a species belonging to the genus Fallopla Adans. were investigated and described by using a stereo microscope. Its scientific name was identified to be Fallopla multiflora (Thunb.) Haralds. (Vietnamese name: ha thu o do). The microcharacters of the stem, folium and powder of the aerial parts were also described.
Keywords: Fallopla multiflora, morphological characters, microscopical characters.
Dat van de
Hd thil d dd (Fallopla multiflora (Thunb) Haralds.) la d y g c lidu d y g c s d dung de ehda mpt so bpnh nhy: suy nhyge t h i n kinh, thilu mau, xa vda ddng mgch, tang huylt dp, tdo bdn... theo kinh nghipm c l truyln^^'^l Dygc lieu nay ehii y l u mpe t y nhien d mpt so linh vung Tdy Bac nhy. Lai Chdu, Cao B l n g , Ha Giang... va hidn nay dang bj thu hdi mpt each l y do nen san iygng giam rat nhilu so vdi tryde day. Hd thii d do la loai thupe ehi ha thil d {Fallopla), hg Rau ram (Polygonaceae).
d Vipt Nam, hp Rau ram da d y g c nghien edu kha ky v l phdn ioai thye vat va hidn da cd tai lieu thye vgt ehi ridng cho hp ndyi^i. Tuy nhidn, qua tham khao tai lipu ndy, thdng tin ve eay hd thii d dd chi tdp trung md ta dde d i l m hinh thai ciia loai. Bdn cgnh do, trong lai lieu Dygc d i l n Viet Nam IV lai ehi edng bo thdng lin ve dae diem vi phau r i vd dde d i l m bpt rd ha thii d ddi'i. Vi vdy, nghien edu nay d y g c thye hien nhdm xac djnh ehinh xac doi tygng cho nhdng nghien cdu t i l p theo ddng thdi eung cap thdm thdng tin v l dae d i l m vi hpc p h l n tren mat dat ciia cay ha thu d do, gdp phan tidu c h u i n hda lodi eay nay.
N g u y e n v a t l i e u , t h i e t bj v a phu'O'ng p h a p n g h i e n CLFU
Nguyen vdt lieu
- Nguydn lieu: eay ha thii d dd thu hdi tai xa Ddo San, huyen Phong Tho, tinh Lai Chau vao thdng 01/2015 va d y g e ky hidu ma lieu ban la HVD-007-15.
- Hda ehat: acid acetic, xanh methylen, do son phen, eloramin B.
Thiet bj
Kinh h i l n vi Leica 400x (Ddc), kinh liip soi noi Cari Zeiss, mdy dnh ky thugt so Canon A480 (Nhat)...
P h y a n g phap nghien cdu - Phan tich hinh thdi:
Quan sat va md la dae d i l m hinh thdi thyc vdt v l : dang song; than; Id (hinh dang phien, ehdp, gdn, goe, cuong, kich thydc...); hoa (dgng cym hoa, vj tri cum hoa, kieh thyde, Id bac, bd nhj, b0 nhyy...);
qua vd hat (hinh dang, mdu sde, kieh thyde.,.) ^y - Thu hai, Idm tieu ban mau cay kho: Thu hai vd Idm lieu ban cd d l y dii bO phdn sinh san.
- Giam djnh ten khoa hgc cua loai nghien cdu: d y a tren tai lipu tham khdo va so sdnh vdi lieu ban mdu.
- Phan tich dae diim vi phau: tieu bdn vi phau than d y g e edt ngang d doan thdn thd 3 tinh I d d i u canh. Tieu ban vi phdu Id d y g e eat ngang d vi tri khoang 1/2-1/3 d y d i gan goe eua Id trydng thanh. Quan sal, md td vd chyp dnh cac dae d i l m vi phdu qua kinh h i l n vi'^'.
- Phan tich d$c diem bpt: bdt p h l n trdn mgt ddt eiia eay d y g c say khd, nghien mjn vd lam tieu ban bgt. Quan sal, md Id va chyp anh ede ddc i S i m qua kinh h i l n vi '^'.
K e t q u a v a b a n l u a n
Dae diem hinh thdi va gidm djnh ten khoa hgc cua loai co ky hipu HVD-007-15
TAP CHI DlTgfC HQC - 7/2015 (SO 471 NAM 55)
• Nghien ciru - Ky thuat
Cay thao, leo. Than trdn, nhan, dde; than non mdu xanh, than gid han ed nda mau xanh va nda mau dd thdm. Cd dogn than moc t h i n g , cd dogn dang xodn. Than hai loi Idn d dot, cde dot cdch nhau khoang 5,0-9,0 em. Mdi dot ehi phdn 1 nhdnh vdi 2-3 la mge d dot ed phdn nhdnh.
La dan, mgc so le; cuong la nda mau xanh va nda mdu do tham, dai 1,5-4,0 em; bp ehia mdng, mdu ndu nhgt, ddi khoang 0,5-1,0 cm. P h i l n Id hinh mui ten dai tdi 7,0 cm, rdng tdi 4,0 em; goc Id hinh tim; mep nguyen; chdp thudn nhpn; mgt Id nhan, phia tren mau xanh t h i m , phia d y d i mdu xanh nhat. Gan Id hinh chdn vjt vdi mdt gdn ehinh va 5-7 gan ben vdi 2-4 gdn ben x u l t phat ty g l c la.
Cym hoa moc t d ndch la hoge dau ednh; moc thanh chiiy, phan nhanh, dai khoang 15-20 em.
Hoa lydng ti'nh, d y d n g kinh hoa khoang 2,0 mm.
Hoa cd 5 eanh mau trdng trong do 3 cdnh cd g d noi phia sau, kich t h y d c khodng 0,8 x 0,5 mm;
nhj 8, ehl nhj dai khoang 0,8 mm, g i p 3 l l n bao p h l n , bao p h l n 2 ngan; nhj 1, niim nhyy xe 3 thiiy; bau hinh bdu dye cd 3 eanh, b i u tren kich t h y d e khoang 0,6 x 0,4 mm. Nhi dinh vao p h l n goe cua canh hoa vd bau dinh vao phan goe ciia bao hoa. Cuong hoa dai khoang 1,0 mm, nhan, khdng cd Idng. La bae mau nau dd dm lay 2-3 hoa. La bde va thdn bdng hoa ed Idng gai thd rap.
Mua hoa thang 9-10, miia qua thdng 11.
Dya vao quan sal eae dgc diem hinh thai, tham khao tdi lieu ' ^ ' ^ k l t hgp vdi k l l qud gidm dinh cua PGS. TS. Vu Xuan Phyang (Vipn Sinh thai va Tai nguyen sinh vat, Vipn Han Idm Khoa hpe va Cdng nghe Viet Nam), lodi ed ky hieu HVD-007-15 d y g e xac djnh ten khoa hpe la Fallopla multiflora (Thunb.) Haralds.. ldn Viet Nam la: ha thu d dd.
Dae d i l m hoa va lieu ban m i u ciia m i u cay ha thil d dd thu hdi d linh Lai Chau d y g e minh hpa d hinh 1.
z^ r .i-
Hinh 1 : Anh ddc diem hoa vd tidu ban miu cua cay ha thu 6 do 1. Cum hoa; 2. Than ci,im hoa; 3. Bong hoa; 4. La bic; 5 Canh hoa;
6. Nhi hoa, 7. Nhuy va bau; 8 Tieu ban mau cay Ddc diem vl phau than va la ciJa cay ha thu
d do
Dae diem vi phdu than
Hinh 2: Anh ddc diem vi phdu thdn cCia cay hd thu 6 do
Mo ta: mat cat than hinh da giac, t d ngoai vao trong cd: Idp bleu bi (1) gom mdt hang t l bao x l p deu dan. Ngay d y d i Idp bieu bi Id Idp md m i m vd (2) gdm 6 hang t l bao hinh Iron, kich t h y d e khdng ddu. Vong md edng (3) xep ngay sat d y d i Idp md mdm vd gom 4-6 hdng te bao hinh trdn, thanh day hda gd, kich cd khdng deu nhau. Ben trong vdng md cdng la cac bd libe-gd (4). Phan libe d phia ngoai gom cac bd libe xep sil nhau, thinh thoang xen gida cd dam mo mem Phan go d phia trong vdi cac te bao hinh trdn to, thdnh day hda go. Xung quanh sgi g l la md cdng (5) gom cac t l bao hinh trdn nhd, thdnh day hda go.
TAP CHi DirOC HQC - 7/2015 (SO 471 NAM 55)
• Nghien ciru - Ky thuat
T i l p den la Idp md m i m rudt (6) gom nhyng mdng, kieh c d khdng d i u . Trong eiing eua thdn te bao hinh Iron hogc hinh bau due lo, thanh cd md k h u y l t (7) Id mdt khoang trong rdng.
m 4
Hinh 3: Anh ddc didm vi phdu gan la (A) va phidn id (B) cua cay ha thu d do
Dae diem vi phdu gan id (hinh 3A) Gan Id loi d hai mat tren vd d y d i . Ngoai Idp b i l u bi tren cd eae Idng che c h d (1) hinh chudng. Bieu bi tren (2) vd b i l u bi d y d i (3) la m i l hang t l bdo x l p deu dgn. Md mem (4) xep ngay d y d i bieu bi trdn va d y d i , gdm ede l l bdo hinh trdn, thanh mdng, kieh t h y d e khdng d i u , ed ehda n h i l u tinh the caIci oxalat hinh eau gai. Cf ehinh gida gdn la ed 4 bd libe-go (5) x l p thanh vdng trdn vdi p h l n libe d phia ngodi va
p h l n go d phia trong.
Ddc diem vi phau phien la (hinh 3B) Gida hai Idp b i l u bi (1) tren va d y d i Id Idp md mem dong hda (6) gdm nhdng te bdo hinh trdn, thanh mdng, kieh t h y d e khd deu nhau. Cd 1-2 hdng t l bao x l p d y d i Idp b i l u bi trdn chda nhieu lye lap.
Dae d i l m bot cua cay hd thu 6 do D0C diem bpt thdn
P
Hinh 4: Anh ddc diem bot thdn cOa cdy ha thd d do Mo ta: bdt than eay hd thu d do cd mau nau
nhgt, khdng mui. Soi d y d i kinh h i l n vi t h i y mdt so dge d i l m gom: mdnh bieu bi than (1) gom nhdng te bao hinh c h d nhal, khd d i u nhau ve kieh t h y d e , mau vdng nhal; manh md mem (2) gom n h y n g l l bao hinh da gidc, thdnh cellulose mdng; manh mach xodn (3); mdnh mgch diem (4); bd sgi (5) dai, thanh day; eae
hgt tmh bpt (6) d a n hoge ty Igi thanh ddm cd dang hinh c l u , hinh chudng hodc hinh qugt, kieh t h y d e khoang 0,01-0,015 m m ; tinh t h l caIci oxalat hinh c l u gai (7) kieh t h y d e khodng 0,03-0,06 m m ; t l bao mo edng (8) cd thdnh day hda g d , khoang hep; mdnh mang mdu (9) cd mau vang.
66 TAP CHI DU*CK: HQC - 7/2015 (SO 471 NAM 55)
Nghien CIJU - Ky thuat
Dae diem bpt la
1 1 ^ # '
©
Hinh 5: Anh ddc diem bpt Id cua cdy hd thO 6 do Md ta: bpt Id eua cay ha thti d dd ed mdu xanh
ndu, khdng miii. Soi d y d i kinh h i l n vi t h i y mgt so dgc d i l m gom: manh b i l u bi (1) mang Id khi kieu tryc bao; mdnh md mem (2) gom nhdng t l bao hinh da gidc, thanh cellulose mdng; manh mgch xodn (3); bd sgi (4) dai, thanh day; cac hat tinh
bdt dan ed dang hinh eau (5), hinh chudng (6) vd hinh mgt n h i n , kich t h y d c khoang 0,025-0,05 mm; tinh the ealei oxalat hinh cau gai (7) hoge hinh khoi (8), kich thydc khodng 0,04-0,06 mm;
manh mang mau (9) cd mau vdng tham.
Ddc diem bpt hoa
Hinh 6: Anh ddc didm bpt hoa cua cay ha thu 6 do Mo ta: bpt hoa eua edy ha thu d dd ed mau
vdng ndu, khdng mtii. Soi d y d i kinh h i l n vi thay mpt so dge diem gom: mdnh b i l u bi mang gai (1); manh b i l u bi mang lo khi (2) k i l u trye bao, manh md m i m (3) gom nhdng t l bao hinh da giae, thanh cellulose mdng; manh mgch xodn (4); bd sgi (5) dai, thdnh ddy; tinh bgt (6) dan ho#ely lgi thdnh ddm ed dgng hinh bau due, hinh chudng, kieh t h y d e khodng 0,01-0,02 mm; tinh the caici oxalat (7) hinh khoi kieh thydc khoang
0,01-0,02 mm; t l bdo md edng (8) hinh ehd nhgt, hai thudn d hai dau, thanh d l y hda go, khoang hep; manh mang mau (9) cd mau vang t h i m ; hgt p h l n hinh (10) b i u dye, mdu ndu xanh, cd mpt ranh sdu, kieh t h y d e dai khoang 0,04 mm vd eho rdng nhat khoang 0,02 mm.
K e t l u a n
Qua quan sat dde diem hinh thai, ddi e h i l u tai lieu tham khao k i t hgp vdi k i t qua giam TAP CHi DUQC HQC - 7/2015 (S6 471 NAM 55)
Nghien CLFU - Ky thuat
djnh eua PGS. TS. Vu Xuan P h y a n g , da xac djnh d y g e loai nghien edu cd ten khoa hpc la Fatlopia multiflora (Thunb.) Haraids., thude hp Rau ram (Polygonaceae). Nhdng dae d i l m bpt va dae d i l m vi phdu eua than, la loai nay eung da d y g e md ta gdp p h l n tieu ehuan hda d y g e lipu ndy.
L d i cam o r
Ndi dung ciia nghien cdu nay thude d l tai ma sd KHCN-TB.04C/13-18. D l tai nay dyge tai trg bdi Chyang trinh "Khoa hpc va Cdng nghp phyc vy phdt trien ben vdng vung Tay Bdc" ma sd KHCN-TB/13-18 do Dgi hpc Quoe gia Ha Npi la c a quan ehii tri.
Tai lieu tham khao 1. Bo Y te (2009), Dupc didn Vipt Nam IV. chuydn lupn Ha thu 6 do (r§), Nxb. Y hpc
2. U Dinh Bfch, Tran Vdn On (2007), Thwc v$t hgc, Nxb. Y hpc, trang 42-138.
3. Nguyin Hdu Dgi, Nguyin Thj Bd (2007), Thyc vat chi Viet Nam, ho Rau ram (Polygonaceae), Nxb Khoa hQC va Ky thuat, trang 121-132.
4. Do Tat Lgi (2001), NhOng cdy thudc va vj thudc Vi^t Nam, Nxb. Y hpc. 2001, trang 355.
5. Nguyin Viet Thdn (2003). Wem nghipm Dupc lieu bing phwang phap hiin vl (tpp 1), Nxb, Khoa hpc va Ky thuat, trang 13-20.
6. Vien Dygc lipu (2006), Cdy thudc va dpng vdt lam thuoc & Viet Nam, Nxb. Khoa hpc va Ky thugt.
7. Hou Wu Shu. Li Anjen, Chong-wook Park (2003), Flora of China 5, Fallopla Adanson, pp. 315-318.
{Ngay nhan bai: 22/04/2015 - Ngay duyet ddng: 02/07/2015)
D j n h l i r o n g d o n g t h o i . . . (Tidp theo trang 42) Dp ddng
Mdc dg dung so voi - nong do DL
80%
100 % 120 % Trung binh
Bang 1 6: Khao sat do dung cua Guaifenesin
Tyl4pht/chdi
%
98.90 99.04 99.02 98.99
RSD (n=3)
(%)
0.498 0.139 0.171 0.280
1 phuang phap Dextromethorphan hydrobromid
Tyle phuc hdi
%
99.01 99.25 99.82 99.36
RSD fn=3J 1%) 0,451 0,550 0,072 0,510
CeSiizin hydroclorid Tyl^phiichdi
%
99,05 99,09 99,18 99,11
RSD (n=3) 0.112 0.535 0.255 0.310
= so tan lap tai thi nghiem RSD ciia li le phuc hoi d eae mdc < 2 %, ti
Id phuc hoi eua ede c h i t dgt RSD < 2. P h y a n g phdp dgt yeu c l u dp ditng.
Ket luan
Quy trinh djnh lygng dong thdi guaifenesin, dextromethorphan hydrobromid va cetirizin hydroclorid bang HPLC pha dao, dau dd PDA vdi vipc s d dyng dong thdi hai b y d e sdng (290 nm vd 232 nm) va pha ddng la dem phosphat pH 4 -methanol - aeetonitril (55:30:15) ed dp dge hidu.
dg chinh xdc va do dung eao. Phyang phap dan gidn, nhanh chdng, ed the dp dung djnh l y g n g t h y d n g quy dgng bao c h l vien nang ed thdnh p h l n 3 hoat chat guaifenesin, dextromethorphan hydrobromid va cetinzin hydroclond t y a n g t y .
Tai lieu tham khao 1 Le IVIinh Tri (2012), Hda dupc tdp 1, Nxb Gido due \fiet Nam.
2. United Stales Pharmcopeia 37.
3. ICH Q2A (2005), "Validation of analytical procedures. Text and methodology Intemational conference on harmonization", Geneva.
4. S. Joshi. C. Bhatia, C. S, Bai, S. M. Rawat (2012),
"Quantitation of dextromethorphan and levocetirizine in combined dosage form using a novel validated RP- HPLC mettiod", Indian J. Pharm. Sci., 74 (1). 83-86
5 G. Grosa. E, Del Grosso, R. Rsso. G. Allegrone (2006).
"Simultaneous. stat)Jlity indicating. HPLC-D/<D determinatk)n of guaifenesine and methyl and propyl-paraben in cough symp". J. Phanv. Bkyned. Anal, 41.798-803.
(Ngay nhdn bdi: 03/04/2015 - Ngay duypt ddng: 02/07/2015)
TAP CHi DlTQC HQC - 7/2015 (S6 471 NAM 55)
