KHOA HOC C 6 N G N G H E
MGHI EIM CUU PHAT TRIEIV GIOMG LUA HAT TRUMG BiMH T P G l THOMG QUA MARKER PHAM TLT
KET HOP LAI HOI GIAO CAI TIEM
Nguyen Thi Lang', Bui Chi Bmi^
T6MTAT
Phirong phap chon giong bang chi thi phan tu phoi hop vai lai h6i giao bang phirong phap cai tien dugc thuc hi?n de lai tao giong quy tu nhieu gen mong mudn tir Jinm!byeo/SP6. Giong TPGl co thai gian sinh truang thupc nhom Al (90-95 ngay). Chieu cao cay 100-105 cm va do dai bong 25-26 cm. So vai gidng khac. TPGl CO sd bong tren bui tr^ng binh (10-15 bong/bui). Sd hat chac tren bong 110 hat. Ty le hai lep trong vu dong xuan (DX) khoang 14.2%. thich hpp cho ca hai vu DX va he thu (HT). Kha nang thu phan rat manh Khdi lupng 1000 hat dat 23,8 g, vi vay TPGl dupc xep trong nhom hat gao trung binh. Xet ve chi sd thu hoach (HI), TPGl co gia tri HI tuong ddi cao, dat 0,57, Xel ve ham luong amylose dat 19.7%. la nhom hat gao cd ham lupng amylose thap. Kich ca hat gao c6 chi^u dai tu 5.96 - 6 mm. Day la gia tn quan trpng cho xuat khau hat gao trung binh. TPGl cd ti6m nang lan trong vu dong xuan nang suat dat 5-7,5 tan/ha tren 7 didm va vu HT d^t 5,00 tan/ha tren 5 diem. TPGl hoi khang den hoi nhiem ray nau (cap 3-5), hai khang vai dao dn (cap 1-3). Day la gidng cd linh thich nghi rong. nang suait dn dinh. Gidng TPGl cd hat trung binh dap ung duCTc nhu cau cua cac thi tnrdng khd tinh.
Tu khia: Di truyin, ebon gidng, ebon gidng bang cbi tbi phan td. SSR, cay lua Orysa saUvaL.
l.fiATVANfl^
Thuc hien tai ca cau nong nghiep vii'a phai theo Cff d i e till tnrong vua phai dam bao cac muc tieu ve nang cao thu nhap, ddi song cho nguoi dan nong thon theo huong phat trien ve so luong va chii trpng nang cao chat lugng, hieu qua the hien bang lgi nhuan, gia tri tang them, dap ung cac nhu cau ngay cang cao cua xa hoi. Nhu cau gao hat tron, kich thuoc trung binh dac biet la liia gao co ham luong amylose thap la nhu cdu tieu thu trong va ngoai nuoc. Cac loai giong dac san dien tich tang dang ke nhu hat gao Japonica va so loai gidng khac co dien tich it nhung do thi tnrtmg tieu thu dn dinh va gia cao nen dien tich cung tuong doi on dinh nhu lua Nhat.
Nhu c^u tieu tliu gao khong phai chi dimg lat a gao hat dai ma can tim ra cac gidng lua co hat tron va hat trung binh voi pham chat cao la nhu cau can thiet cua thi truong kho tinh. Trong mSy nam gdn day nhu cau gao hat trung bmh bat dau gia tang nhu thi tiorcmg ciia My, Singapore, Hong Kong, Uc,... do do su can thi^t da dang nguCn gen la nhu cau rdt cap thiet trong san xuat. Hien nay Tap doan Sunnce rdt cdn giong ltia hat trung binh co pham chat ngon de xudt khdu ttri nhieu nuoc. Do do muc tieu ciia d^ tai la chgn tao duoc gidng liia hat ti'ung binh, chat luong
cao, chong chiu sau benh hai chinh, thich nghi rong, dap lirng yeu cau thi truong trong nutrc va xudt khdu, mang lai hieu qua cao cho ngudi san xudt va kinh doanh liia gao cr DBSCL va cac viing lan can.
2 . VAT LfiJ VA PHUDNG PHAP NGHIBV CUU 2.1. V$t lieu
Thi nghiem dugfc tien hanh voi gidng Jinmibyeo lam me va gidng SP6 la nhom Japonica lam bo. Cac qudn the the he con lai F,, BCiFj, BCaF,, BC.F.,...BC.Fj. Cac marker RM201, RM223 de chgn Igc tinh trang amylose va miii thom quan the the he con lai. Cac thiet bi phuc vu cho chon gidng thdng qua marker phan ttr. Su dung marker trong chon tao cac the he con lai.
2.2. Phuong phdp nghien cuu
Qudn the lai theo phucmg phap hoi giao dugx: bd la SP6 va me la Jmmibyeo. Qudn the BC2F^ duoc dua vao de danh ddu bang marker phan Oi de chon giong CO nang suat cao va va dua gen miii thom vao qudn the cuaTPGl.
Ly trich DNA va xet nghiem microsatellite theo Lang, 2002.
2.2.1. Qua trinh chpn chon tao gidng TPGl Gidng liia TPGl, duoc chgn lgc tir to hgp lai J mm ibyeo/SP6//Jinmibyeo. Su dung phuong phap ' Vicn NsinC'n cim Nong nghiep Cong nghe cao DBSCL
- Vicn Khod hpc Ky thuat Nong nghiep mien Nam
KHOA HOC CONG NGHE lai hoi giao voi chi thi phan tu RM 3475 (nhiem sac
the sd 3). RM 223 (nhi^m sdc the so 8).
Phirong phap danh gia kiiu hmh
Gidng Ilia TPGl dugc chon loc lir to hap lai J inm ibyeo/SP6//Jinmibyeo. Jinmibyeo la gidng du nhap tir Han Qudc, thuoc loai hinh Japonica. khang sau benh va phen, man duoc diing lani me. Giong liia SP 6 dugt: du nhap ttr \'icn Nghien cuu Liia Qudc te dugc diing Iam bd, la gidng lua cimg cay, de nhanh tot, nang sudt cao dn dinh. Gidng liia TPGl bdt dau dugc lai tao nam 2013, vu DX 2013-2014 lai tao Fl.
HT2014 lai BCIFI, XH 2014 Iai tao BC2F1. Danh gia BC2F2 U-ong vu DX 2015-2016.
Vu DX 2015- 20ie Iai tiep BC3F1, HT 2015
^ C 4 F 1 , XH 2015 tu thu BC4F2; DX 2016-2017 tu thu p C 4 F 3 ; HT 2016 tu thu BC4F4: XH 2016 so sanh 5BC4F5 TPGl dugc dua khao nghiem Qudc gia HT
E
I7, DX 2017-2018 va HT 2018.Theo ddi cac chi tieu ve nong hgc. sau benh va
^ chat Uieo tieu chudn IRRI 1996.
2.2.2. Kliao nghiim caban
Ap dung quy chuan ky tiiuat Qudc gia ve khao aghiem gia tri canh tac va sii dung (khao nghiSm
^CU) ciia gidng lua (QCVN 01-55:2011/BNNPTNT)
do Bg XN & PTNT ban hanh ngay 5 thang 7 nam 2011.
2.3. 'Didi gian, dia di^m nghien ciiu Nghien cthi dugc ti^n hanh tai Cdng ty Trach nhiem Him ban mdt thanh vien PCR va \\kn Nghien cuu Ndng nghiep Cdng nghe cao DBSCL
3 . KET QUA KMU NGHBV!
3.1. Chgn gidng bang chi thi phSn hi Khuech dai DNA bang phuong phap PCR-SSR vdi marker RM223.
Gene muc tieu dugc chgn de thuc hi?n thi nghiem nay la gene khang man tren nhilm sac the so 8 (salto). Gene lien ket chat tren nhi^m sdc th^ sd 8 duoc danh ddu boi marker phan tir RM223. Gene nay cd lien ket voi gen miii thom va vdi gen chdng chiu man d' giai doan ma va phat due (Nguyen Thi Lang va ctv. 2001). Marker RM223 dugc sii dung lam marker danh ddu, marker nay cd kich thudc 200-220 bp va duoc diing lam khudn DNA de thi^t lap cac cap pnmer dac hieu. Cac cap pnmer nay se khudch dai dtroc cac doan DNA nhd hon nhd' phtrong phap PCR.
Cac doan DNA nhd nay dugc ggi la SSR. Sau dd tien hanh kiem tra viec khuech dai tren gel agarose 3%
trong dung dich TBE IX. Ket qua the hien d'hinh 1.
^Hinh 1. Sdn phdm PCR cita chi thi phan tii SSR 223 tren 101 gidng chdng chiu man, lien k^t vdi gene khdng m ^ tren nhiim sdc the sd 8, vi tii hai bang 220 bp va 200bp, tren gel agarose 3%
| v Ghi cbu: M: la marker chuan
P * ' Tuong tu phan tich kieu alen ciia chi thi RM3475 ghi nhan kich thuoc phan tir cho vi tri cua SP6 la Marker RM34751 dtroc su dung Iam marker danh ddu, mai'kcr nay cd kicli thudc 220-230 bp va dugc
diing Iam khudn DNA de thiet lap cac cap primer dac hieu. Dieu nay cung phii hgp vdi Bin Chi Biiu va ctv, 2016. Ket qua ghi nhan da sd cac gidng khdc biet voi gidng Jinniibyea/SP6//Jinmibyc'0.
|M:::::: : ! : 8 : i : : 8 : : asassa»»»""»«»«f • • - • • •
Hinh 2. San phdm PCR ciia chi thi phan tii RM 3475 tren 50 dong lien k^t vdi gene chiu nong trSn nhiSm sdc th^ s6 4, vi tin hai bang (220-230 bp), tirSn gel agarose 3%
Ghichu: .M. la marker chuan
KH&A HOC CONG NGHE 3,2. So
STT 1 2 3 4 5 6
s4nh nang suSt va thtoh p h i i nSng suat trong TO DX 2015-2016 va HT 2016 B a n g 1. N a n g SuSt v 4 thSnh o h i n n S n ^ Slrft Cl'm h n mrfncr lai .sanh t m n a mi H T 9016
Dong TLGl HATRI200 DSl TMG342 TPGl DS12
TGST (ngay) 100b 110c 125e 120d 92a 118c
Cao cay (cm) I l i a 114a 125d 115b 119c 116b
Choi/bui (choi) 15a 12ef 15a 14cd 15a llfg
Hat chdc/bong 169hi 168ij 126g 177g 186e 159m
KLIOOO hat(g) 25,7a 23,3a-e 23,6abc 23,4a-e 23,7a-e 23,4a-e
%hat lep 12,lkl 15.0f-i 17,0cde 20,0b 11,11m 14,0hij
NSTT (tan/ha) 6,1a 5,7a-d 3,9e-h 6,2a 5,7a-d 5,5a-e Ket qua b bang 1 cho thay, ve dac tinh ndng hgc,
da sd cac giong deu cd TGST ngdn bien dgng trung binh tir 90-125 ngay, ddi voi gidng TPGl bieu hien TGST ngdn hon chi 92 ngay va chieu cao cay trung hinh hr 119 cm. Xet ve sd chdi/bui nhin chung da sd cac gidng deu cd sd bdng/bui kha, tuy nhien gidng TPGl cd sd choi cao (>10 chdi) va sd bdng/m^ tuong
ling bieu hien cung kha cao 324 bdng. Thanh phan nang suat cua gidng TPGl trong vu HT 2016 cd ty le lep thdp va sd hat chdc/bdng khd cao 186 hat. Ben canh do, tuong ting vdi sd hat chdc/bdng cao thi khdi lugng 1000 hat (23,7 g) va nang suat cua gidng TPGl bieu hien kha cao dat 5,7 tdn/ha cao hon cd y nghia so vol gidng ddi chtrng chi DS 1 la 3,9 tan/ha.
Ten gidng TLGl HATRI200 DSl TMG342 TPGl BL12
Bang 2,
% g a o liit
81,6 ah 83,4 a 83,4 a 83,5 a 80,3 ab 79,3 ab
Ty \i xay xit c i a c4c gidng trong vu DX 2015-2016
% gao trang
75,5 b-d 76,3 a-c 74,9 b-e 76,7 ab 74,8 h-e 73,6 h-g
%gao nguyen 50,7 a-c 50,4 a-c 50,2 a-c 49,7 a-c 51,9 ab 51,6 ab
Dai hat (mm) 7,4 a 6,3 h 5,9 c 6,5 h 6,2 b 6,1 h
Rdng hat (mm) 3.2 h-d 3,1 h-d 3 b-d 3,1 b-d 3,2 M 3,1 b-c
Phytic acid
2 2 2 2 2 3 Ket qua a bang 2 cho thdy, trong vu DX 2015-
2016, TPGl cho ti le gao ti'dng (74,8%) va ti le gao nguyen (51,9%) kha cao, chieu dai hat la 6,2 mm va ham lugng acid phytic la 2.
Ket qua danh gia phan img ctia gidng TPGl ddi vol virus benh vang lim, liin xodn la nhan thdy gidng TPGl cd phan img khang tuong ddi (duoc danh gia cap 3 ddi vdi 2 Ioai virus nay). Ben canh dd, gidng ddi chung TNI nhiem vang liin hoan toan.
Da sd cac gidng cho phan ting Idiang tii cdp 1-3 ddi voi rdy nau va dao dn. Gidng TPGl trong vu DX 2015 - 2016 cho phan img khang tiimg binh voi ca hai Ioai dich hai nay.
Ket qua danh gia pham chat gidng lua TPGl ti-ong vu HT 2016 ghi d' bang 3 cho thdy gidng nay
tuong ddi mdm com cd ham luong amylose trung binh 17,1%, do bac bung cdp 3.
Hinh 3. Mat gao gidng ltia TPGl
KHOA HOC C O N G NGHE
TT 1 2 3 4 5 6 7
Bang 3. Chi ti«u phim chat cua bd eidng lua tii& vong vu hi ihu 2016 Gidng
TLGl TLG2 TPGl TMG432 BL12 DSl 1R50404
cn
LSD 0,05
Ham luong amvlose (%)
21,3 19,1 17,1 16.5 16,7 18,1 25,23
4,16 1,64
Do ben gel (nun)
75,2 75,1 75,6 74,5 75,4 75,6 48,26
1,52 2,04
Nhiet trd hd (cap)
3 3 5 5 5 5 J
Mui thom (cap)
0 0 0 0 0 0 0
Bac bung (cap)
0 0 3 1 0 0 7
3.3. Khdo nghidm 6 cdc vung sinh thdi Ket qua khao nghiem nang sudt qua 5 diem cua DBSCL trong vu DX 2016-2017 va HT 2017 gidng Itia TPGl bieu hien nang sudt tuong ddi kha (6.64 I t ^ / h a ) cao hon rat nhiSu khi so vdi gidng ddi chiing ciing khao nghiem (chl 4,80 tan/ha). Trong vu DX j-2016 - 2017, tai 5 diem Cdn Tho, Hau Giang, Tien
Giang, Tra Vinh, An Giang gidng TPGl bieu hien nang sudt rat kha (6.32 - 7,46 tan/ha), trong vu HT
;2017 bi^n ddng tir 5,0 tan den 5,5 tdn/ha.
Sii dung chi sd mdi truong (Ij) bieu trung cho timg dia diem, tren gian do tiJong tac gitra ki^u gen va mdi truong cho thii ttr ttr kem thuan Igi den thugn Igi nhu sau: An Giang > Hau Giang > Can Tha > Long An ndm ti-en true Ij vdi gid tn theo thir tir 0,97; 0.42;
0,32; 0.20: 0,07; 0.00 theo thti tir.
Nhu vay. ti'ong vu DX 2016 - 2017 hau het cac gidng trong dd cd TPGl cd nang sudt trung binh cao hon gidng ddi chiing DSl (6,06 tdn/ha). Su khac nhau ve nang sudt ciia cac giong rat cd y nghia tgi mirc 5% dua vao thang danh gia nang suat thdng qua phan tich Duncan (Bang 4).
B ^ 4. Nang Ten gidng TPGl TPG4 BL12 TMG432 TMG434 BC31-4-l-2-l,5 439 BC4P4-17-2M-200 BC2F3-1034-437
nsi
EMS Trung binh Chi sd mdi hirdng Ij
suit ( t i n ^ a ) Can Tho
7,46 7,53 7,03 6,73 7,46 6,86 7,16 7,26 7.03 0,12 7,17 0,32
dia bd gidng liia khao nghidm t ^ 5 di^m vu Long An
7,20 7,16 6,60 6.66 7,60 7,10 6,93 7,40 6,93 0,134 7,08 0,20
An Giang 7,23 7.16 6.76 6,83 7,73 6,76 7.63 7,50 6,93 0,184 7,17 0,42
Tra Vinh 7.36 7.46 6,16 7,26 7,36 7,03 7,33 7,30 6,53 0,142 7,09 0,00
DX 2016-2017 Hau Giang
6.32 6,06 6,53 6,73 6.73 6,86 7,06 6,96 6,86 0,147 6,68 0,97
TB 7,12 7,08 6,64 6,85 7,38 6,93 7.23 6,74 6,06 7,04
Bang 5. Nang suat (tan/ha) ciia bd gidng liia khao nghiSm tai 5 di^m vu HT 2017 Ten gidng
TPGl TPG4 BL12 TMG432 TMG434 BC3F4-1-2-15 439
Cdn Tho 5,43 5.00 5.20 5.67
Long An 5.57 5.67 5.77 r 540 5.77 5,37 5.70 1 5.2(1
Hau Giang 5.30 5.57 5.67 5.87 5.53 5.93
Tra Vinh 5,60 5,77 5.03 5.37 6.00 6.00
An Giang 6.13 5.67 6,37 5,60 6,10 6,60
TB 5,6fl 5„53 5,60 1 5,58
5.75 5.87
ecHSA esoc C O N G N G H E BC2F3-10-34437
DSl EMS Trung binh Chi sd mdi tnrdng h
4.96 4.30 0.12 5.27 0.32
5,50 5.50 0.134
5,49 0,20
6,27 5,13 0,184
5,70 0,42
6,20 4,97 0,142 5,74 0,00
6.27 5.10 0.147 6.02 0.97
5,84 5,00 5,64 Nhir vay, trong vu HT 2017, hau het cac gidng.
trong dd cd TPGl cd nang suat trang binh cao hon gidng ddi chiing DSl (5 tSn/ha). Su khac nhau vj nang suat ciia cac gidng rat cd y nghia tai miic 5%
dua vao thang danh gia nang suat thdng qua phan tich Duncan (Bang 5).
3.4. Bao c i o k^t qua khao nghidm gidng Ida qudc gia d Nam hd
3.4.1. Bao cao kdt qua khao nghiem gidng Ida vu HT2017
Ket qua khao nghiem cho thay TPGl cd thdi gian sinh trudng 95100 ngay thiip hon gidng ddi chiing DSl (108-112 ngay) va OM5451 (97-102 ngay).
hat chac/bdng 80-90 hat cao hon OM5451 (70-80 hat), ty le lep 19,9% thip hon DSl (22,6%) va OM5451 (24,5%), khdi luong 1000 hat hi 27-28 g cao hon gidng DSl (26-27 g) va OM5451 (25-26 g).
Bang 6. Mot sd dac tinh ndng hoc ciia cac STT
1 2 3 4 5 6 7 8 9
Gidng
BL12 TLGl TPGl TPG2 TPG3 LN26 LN105
DSl OM5451
TGST (ngay) 100-105 100-105 95100 100-105
95100 100-105 100-105 108-112 97-102
Cao cay (cm) 90-100 105115 100-110 105115 100-110 105115 100-110 100-110 95105
gidng lua khao nghidm Sd bdng/m^
240-270 230-260 240-270 260-290 260-290 250-280 240-270 260-290 280-310
Hat chac/bong
80-90 80-90 80-90 60-70 70-80 90-100 80-90 80-90 70-80
nhdm Al (bd 1), vu HT 2017 Ty le lep
(%)
26.5 29.6 19,9 27,1 25,2 19,3 29,1 22,6 24,5
Kl 1000 hat (g) 26-27 26-27 27-28 2526 27-28 26-27 27-28 26-27 2526
Dd Cling cay (1-9)
1 1 1 1 1 1 1 1 1 Kdt qua khao nghiem phan ling cua cac gidng
lua voi rdy nau trong dieu kien nhan tao cho thdy gidng TPGl dugc danh gia d- cap 7,0 (miic dd nhiem) so voi gidng DSl va OM5451 a cap 5,67.
Ket qua khao nghiem phan ting ciia cac gidng Ilia voi benh dao on ti'ong dieu nhan tao cho thdy gidng TPGl dtrgc danh gia d cap benh 1 (muc do khang). Nhu vay, gidng lua TPGl khi thit nghiem trong dieu Iden ngoai dong se it chiu anh hudng vdi benh dao dn.
Ket qua khao nghiem nang sudt qua 5 di^m cua DBSCL ti-ong vu he thu 2017 gidng liia TPGl bieu hien nang sudt tiJong ddi kha (4,71 tan/ha) cao hon rdt nhieu khi so vdi giong ddi chung cimg khao nghiem DSl (chi 3.41 tdn/Tia). Tuy nhien, tai 5 diem Tien Giang, An Giang, Cdn Tho, Kien Giang, Binh Thuan gidng TPGl bieu hien nang suat rat kha mac dtt trong vu he thu (bien ddng tii 4,03 tan den 5.27 tdn/ha) va dat nang sudt cao nhat (5,27 tdn/ha) khi khao nghiem tai diem Kien Giang va thdp nhdt (4.03 tan/ha) khi khao nghiem tai diem Tien Giang.
STT 1 2 3
4_J
Bang 7. Nang su^t (tan/ha) cua cac gidng liia khao nghidm nhdm Al (bd 1) vu HT 2017 CTrung tam Khao kidm nghidm Qudc gia, 2017) Gidng
BL12 TLGl TPGl TPG2
Diem khao nghiem Tien Giang
3,53 3,13 4,03 4,47
An Giang 4,11 5,02 5,00 4,81
CdnThff 3,14 3,48 4,04 3,19
Kien Giang 4,83 4,74 5,27 4,65
Binh Thuan 4,60 5,33 5,20 5,03
Tmng binh 4,04 4,34 4,71 4,43
KHOA HOC CONG NCHI 5
6 7
TPG3 LN26 LN105 DSl OM,5451 CV%
LSD 0,05
. I , ' ; : .
4,07 4,63 3.57 4.52 4.1 0,28
4,87 4,48 5,02 3,74 5,42 5,1 0.45
3.93 4.40 4,16 2,52 4,63 7,5 0,51
5.47 5.S2 4.81 3.81 4.91 10.0 0.86
5,50 5.63 5,27 5,43 U . l 1,04
4,62 1 4,88 4,78 i 3,41 4,98 i
1 3.4.2. Ket qua khao nghiem Qudc gia 0dng liia
vu DX 2017 - 2018
Ket qua khao nghiem nang suat qua 5 diem cua DBSCL U-ong vu DX 2017-2018 gidng liia TPGl dat nang sudt trung binh cao (7.10 tdn/ha) cao hon rat nhieu khi so vdi gidng ddi chimg cung khao nghiem DSl (6.10 tdn/ha) va ()M54.S1 (6,31 tan/ha). Tai 5
didm Tien Giang, An Giang, Can Tho. Kien Giang, Bmh Tiiuan gidng TPGl bieu hien nang suat cao trong \"u DX (bien ddng tir 6,03 tan den 7,93 tan/ha) va dal nang suat cao nhat (7,93 tdn/ha) khi khao nghiem tai diem An Giang va thdp nhdt (6,03 tdn/ha) khi khao nghiem tai diem Binh Thuan.
iJngS.
TT
1 2 4 5 6 7 8 9 10 11 12 13 14
Nang suit (til/ha) Cuing
(•,KG35 TPGl ML21,^) HATRI31 HATRI 61 TG7 OM407 HATRI 33
PY2 HATRI 32
PCR 3 HATRI 62 OM5451
DSl CV%
LSD „ „
cua cic gidng liia khio nghidm nhdm Al (bd 1) vu DX 2017-2018 Tien
Giang 7,09 6,93 7,47 5,93 6,83 6,67 6,88 7.20 6.03 6.13 6.61 5.49 5,25 5.50 11.1 1.15
Diem khao nghiem An
Giang 6.92 7.93 6.52 6,26 6.40 6.48 6.51 6,09 6,81 6,22 6,58 6,38 7,77 7.9 0.81
Can Tha -7.79 7.64 7,70 7,85 7.22 8,19 7,55 7,26 7,68 7,41 6,94 7,77 7.46 5.62 5 5 0,66
Kien Giang 7,50 '6,95 6,35 6,74 6,98 6.62 6.35 6.63 6.42 6.08 6.10 5.21 5,94 5.50 8.2 0,85
Binh Thuan
6.57 6.03 6.17 7.37 6.60 6.00 6.37 6.27 6.37 .6.17 5.90 6.83 6,50 6,10 10,1 1,03
Trang binh
7,17 7,10 6,84 6,83 6,81 6,79 6,73 6,69 6,66 6,40 6,39 6,38 6,31
Ngudn: Tnmg tam Kliao kiim nghiem gidng. San phim cay trdng Qudc gia 2018 Ket qua khao nghiem mtrc dd nhiem sau benh
ti'en ddng rudng nhu: dao dn, khd van. sau due than.
ray nau cua cac gidng lua vu DX 2017-2018 tai cac diem An Giang. Kien Giang, Cdn Tho cho thay gidng Ilia TPG1 dugc danh gia khang benh dao dn a cap 0-1 (mtic dd khdng vdi benh) tdt hon so vdi gidng OM5451 (cap 0-3), benh khd vdn (cdp 1-3), sau due than (cap 1). ray nau (cap 1) tai 3 diem khao nghiem.
Ket qua khao nghiem nang suat qua 5 diem ciia DBSCL trong vu HT 2018 gidng tua TPGl dat nang
sudl tiJong ddi kha (5,52 lan/ha) cao hem rat nhieu khi so vdi gidng ddi chimg cimg khao nghiem DSl (chl 4.55 tan/ha) va OM5451 (5,06 tdn/ha). Tai 5 diem Tien Giang, An Giang, Cdn Tho, Kien Giang, Tay Xinh gidng TPGl bieu hien nang sudt rat kha mac dii trong vu HT bien ddng tir 4,07 tdn d^n 6,92 tdn/ha. TPGl dat nang sudt cao nhdl (6,92 tdn/ha) khi khdo nghiem tai An Giang va thap nhat (4.07 lan/ha) khi khao nghiem tai Tidn Giang.
KHOA HOC CONC NGHE
Bang 9. Nang suSt (tSn/ha) cila cac gidng Ida khao nghidm trdn nhidu didm nhdm Al (bd 2) vu HT 2018 CTiTing tam Khao nghidm Qudc gia vu hd thu 2018)
TT
1 2 3 4 5 6 7 8 9
Gidng
T P G l LA4 TPG 4 OM426 OM427 OM594 OM418 DSl OM 5451
Tien Giang
4.07 4,47 4.33 4.20 3,93 4,27 3,60 4,47
Didm khao nghiem An
Giang 6.92 6,73 7.03 6.23 6,26 5,32 5,55 5,53 5,63
Can Tho 5,80 4,64 5,48 5,00 5,07 516 4.41 4.35 4.62
Kien Giang
6.08 6.03 5,65 6.19 6,33 5,65 5,86 5,03 5,52
Tay Nmh 4,73 5,07 4,33 5,20 4,93 5.53 4.72 4.25 5.07
Tmng binh
5.52 5.39 5,37 5,36 5,30 5,18 5,14 4,55 5,06 4.KErLUaNVAKIBUNGHI
Gidng liia TPGl cd tm diem la gidng ngon com, deo cd ham luong amylose thap 19,7%, cd TGST 95 ngay ddi vdi Itia cay, 90 ngay ddi vdi lua sa, than ra ciing, kha nang de nhanh khde, bdng chiim, nang suat kha cao nhdt la fa-ong vu DX bien ddng tii' 5.5 -7.5 lan/ha. Dac biet ngoai dac Unh ngon com, ly le gao nguyen kha, gidng TPGl cd chdt luong ngon com nen co the bd sung vao co cdu gidng chdng chiu bien ddi khi hau nhu khd han. Gidng nay cd the phti hgp a nhieu vimg dat canh tac khac nhau dac biel Id a cac tinh Ca Mau. Hau Giang, An Giang, Cdn Tha, Bac Lieu, va Tra Vinh bieu hien nang sudt rat cao.
Chinh vi nhung dac diem un viet ve phdm chat, nang sudl, ciing nhu tinh chdng chiu khd ban ctia gidng TPGl gidng co lhi phdn xual khdu cho san Ijham hat tiling binh.
LOI CAM DN
Tac gia Ccini on Bo Klioa hoc va Cdng nghe va Chuong tiinh Tay Nam ho da tai tra kinh phi cho nghien ctru nay.
TAI UEU THAM KHAO
1. Bill Chi Biai, Nguyt^n Thi Lang, 2000. Mdt sd van dican biit ve gao xuat khau. NXB Ndng nghiep.
TP. Hd Chi Minh.
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3. IRRI. 1996. Standard evaluation system for rice. Phihppines. 52 p.
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8. Sd Ndng nghiep va PTNT cac tinh. Cdn Tho.
2017, 2018.
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KHOA HOC CONG NGHR
TPGl -A PROMISING RICE VARIEIY W i m MEDIUM GRAIN
Nguyen Tlii Lang, Bui Chi Buu Summary
The new advances are applied to production, especially by the method of molecular indicators in collaboration with by improved method are made to the same breeder together many desirable genes from Jinmibyeo/SP6. The same Ume has grown TPGl in Group Al (90-95 days). Height 100-105 cm and panicle length 25-26 cm. Compared to other lines, the number pamle/hiE average (15 panicle). The number of filling from 110. \\Vighl of 1000 seeds reached 23.8 g. Reviews on harvest index (HO. TPGl worth tlie relatively high HI. gaming 0.57. Productivity of TPGl has great potenttal in the winter-spring season, but often for low producbvity in the summer. This is an important value for the average rice gram exports. The yield 7.5 tonnes/ha of winter-spnng season and 5.0 ton/ha m wet season. This is just like having wide adaptabihty, stable yield, this is important just like the average seed the need for countnes to serve in specialized seed, should it be maintained quite long in production in the Mekong delta.
Keywords: GeneUc. plant breeding, marker-assisted selection, .Microsatellite. Orysa sativL Ngudi phdn bi^n: GS.TSKH. Trdn Duy Quj>
Ngdy nhan bdi: 18/9/2019 Ngdy thdng qua phdn bi§n: 18/10/2019 Ngdy duy^t dang: 25/10/2019
