Top chi Khoa bgc Tnrdng Dgi hgc Cdn Tha Phdn B: Ndng nghifp, Thity sdn vd Cdng nghf Sinh hgc: 33 (2014): 61-67
Tap chf Khoa hoc Tru'dng Dai hoc Can Thd website: sj. ctu. edu. vn
KBOABpC
TI LE NHDDEM VA SU" DE KHANG KHANG SINH CUA VI K H U A N VIBRIO SPP.
PHAN LAP Ttr HUYET HEO, NGHEU VA PHAN BENH NHAN TIEU CHAY TAI TINH TRA VINH
Nguyen Thi Dau', Nguyen Thiiy Linh', Ho Thi Viet Thu" va Ha Thanh Toan"
' Trudng Dgi hgc Trd Vinh
^ Khoa Ndng nghidp & Sinh hgc Ifng dung, Trudng Dgi hoc Cdn Tha
•* Truang Bai hgc Cdn Tha
Thdng tin chung:
Ngdy nhdn: 04/03/2014 Ngdy chdp nhgn: 28/08/2014 Title:
Prevalence and antibiotic resistance of vibrio spp.
isolated from swine blood sample, clam and patient with diarrhea in Tra Vinh Province
Tu khda:
Vibrio choleae; nghiu; li li nhiem; di khiing khdng sinh;
Trd Vinh Keywords:
Vibrio choleae, clams, infection rate, antibiotic resistance, Tra Vinh
ABSTRACT
In this study to determine the prevalence of Vibrio spp. on water samples at slaughter, seafood and feces of patients sei-ve for further studies on identification species of Vibrio spp. and their antibiotic resistance.
Isolation of Vibrio spp. was carried out on 300 samples including 160 clam samples, 100 swine blood samples and 40 feces of diarrheal patients collected from Tra Vinh hospital, Tra Vinh province. Results indicated that there were 10% clam samples and 4% swine blood samples contaninated by Vibrio spp. whereas no Vibrio spp. was isolated fi-om diarrheal feces The results ofkirby bauer antibiotic sensitivity tests showed that all of the isolated Vibrio spp. strains weresensitive to norfloxacin (100%), chloramphenicol (70%) but 90% of the isolates is resistant to amoxicillin. Three out of 20 (15%) Vibrio spp. isolates were positive for multivalent antisera (0139. Ogawa, Inaba). 10% (2/20) of them was positive for monovalent antisera (Ogawa and Inaba).
Nghiin cuu ndy nhdm xdc dinh ti li nhiim Vibrio spp. trin mdu nudc tgi ca sd giet mdfthucidrrfidi sdnvd phdn benh nhdn ldm ca sd cho cdc nghiin cuu dip theo vi viec djnh danh lodi thugc Vibrio spp. vd su di khdng khdng sinh cda chiing. Kit qud dd phdn lap vi khudn Vibrio spp. trin 300 mdu, bao gdm 160 mdu ngheu; 100 mdu huyet heo tgi cdc ca sd giit md vd 40 mdu phdn trin benh nhdn tiiu chdy tgi b$nh vien da khoa tinh Trd Vinh. ti li phdn lap Vibrio spp. ldn lu0 tren nghiu Id 10%, tren huyit heo cd pha nudc tgi ca sdgiit md la 4%, chua cd ddu hieu duang tinh tren benh nhdn tieu chdy tgi linh Trd Vinh. Kit qud khdng sinh do cho thdy da sd vi kliudn Vibrio spp. nhgy vdi norfloxacin (100%), vdi chloramphenicol (70%), vd de khdng hodn todn vdi amoxicillin (90%). Cd 15% mdu duang tinh vdi khdng huyit thanh da gid (0139, Ogawa, Inaba), ti l^ duang tinh vdi khdng huyit Ihanh dan gid Ogawa (10%) vd vdi khdng huyit thanh dan gid Inaba (10%) tren nhung mdu benh phdm dugc phdn Igp.
1 G i a i T H I ? U
Thi gidi da trdi qua 7 dgi dich td, tu nam 1817 din ndra 1923 da cd 6 vy dgi dich xay ra, nhimg
dai dich ndy diu bdt dau tir An Dd vd diu do Vibrio cholerae 01 type sinh hgc cd diln gay ra.
Dai dich thii 7 khdc vdi 6 dai dich ttudc, dai dich
Tgp chi Khoa hgc Tnidng Dgi hgc Cdn Tha Phdn B: Nong nghifp, Thuy sdn vd Cdng nghf Sinb bgc: 33 (2014): 61-67 nay do V.cholerae type sinh hgc El Tor gdy ra v i
cd ngudn gdc tii dio Celebes ciia Indonesia nam 1961. Dai dich nay keo dai nhdt vd cd pham vi rgng faon 6 dgi djch trudc do, din nay cdn nhieu nuac thdng bao nhiing dgt biing phat dich ta cung do nguyin nhdn nay gay ra (Vd Van Lugng, 2009).
Rieng Viet Nara di xdy ra 3 dgt dich tiiu chiy cdp nguy hilm: dgt thii nfadt tii ngdy 23/10 - 6/12/2007 d 14 tinh, thdnh phia Bdc vdi 1.878 ca, ttong dd cd 295 trudng hgp duong tinh vdi vi khuin ti; dgt tfaii faai tii ngdy 24/12/2007 5/2/2008 d Hd Ndi vdi 58 ca, cd 32 ca do vi khuin td; dot tini ba, tir ngdy 6/3 din 11/4/2008, da cd 1.335 ca, ttong do 136 ea duang tinh vdi vi khudn td, d 18 tinh, thanfa thugc ca 3 miln Bac, Trung, Nam (Vo Van Lugng. 2009). Nim 2009 - 2010, dich t i lai xudt hiln tai cdc tinfa miln Bac Viet Nam, mgt chung vi khudn V. cholera 0139 dugc phdn lap tir 7 mdu nudc vd dugc dgt ten la V.
cholerae 0139 (Dong Tu Nguyen el ai, 2012).
Tinh Trd Vinh cd vi tri dia ly vdi nhilu nguy co tiim in benh dich ta vi cd bd biln keo ddi khoang 65 km vd ttin dia bdn Trd Vinh cd he thdng sdng chinh vdi tdng cfaieu dai 578 km, ttong do cd cdc sdng ldn la sdng Hdu, sdng Cd Chien va sdng Mdng Tfait, vi the rdt de cho vile luu hinh vi khudn td tir sdng Cd Chien, biin DuyIn Hdi va ciu Ngang.
Cic nghiin ciiu ttong nudc cho thay trieu chiing chinh cua benh ti ttin ngudi li do ddc td ciia vi khuin V. cholerae gdy ra. Vi kfaudn cd mat d cac mdi tiirdng nudc bien va ngudn nudc bi d nhilm, sy thay ddi vl tinh nfagy cdm kfadng sinfa cua V. cholerae 01 d Viet Nam cung dugc gfai nlidn
• ttin mdt sd logi khdng sinh (DePaota, A, 1981).
Di ngdn chan sy lay lan vi nguy hilm cua binh dich td, dgc bilt Id d vung Ddng bang sdng Cuu Long nai cd nhilu dilu kiln de vi khuan lay lan, cbiing tdi nghien ciru "Ti II nhilm vd su de khang khing sinh ciia vi khuin Vibrio spp. tten fauyet heo, ngheu vi pfain benfa nhdn tiiu chiy tgi tinh Trd Vinh", vdi muc lieu xic dinh ti 11 nfaiem Vibrio spp. ttin cdc logi radu pfadn lap; xdc dinfa serotype phd biin luu hinh cd the gay benh cho ngudi vd tt'nfa nhay cam kfadng sinfa ciia cic cfaiing Vibrio spp. phdn lap dugc.
2 VAT LIEU VA PHU'ONG PHAP 2.1 Vat lieu
Cic logi mdi trudng bao quin miu vd nudi ciy vi khuin Vibrio spp. la Gary- Blau" (India), APW:
Alkaline peptone water (India), TCBS:
Thiosulfate-Cittat-Bilei-Saccfaarose (Germany), SNA: Saluie Nutrient Agar (Germany).
Mdi trudng tfauc failn kfadng smh dd: MHA:
Muller Hinton Agar. dTa gidy tdm khang sinfa amoxillin, tetracycline, norfloxacine, chlorampfaenicol, azitfaromycin (Nam Khoa).
Bd dinh danh vi kfaudn Gram dm (Nam Kfaoa): ^ a giiy oxidase, dia gidy ONPG, TSI:
Triple sugar iron, ADH: Arguune Difaydrolase, Tryptopfaan saline....
Bd thuoc nfaudm Gram (Germany), bd khang huyit thanh dinh type V. cholerae (Vien Pasteur TP. Hd Chi Minfa) va nudc Peptone ed muoi NaCl vdi cic ndng do tir 0-10%.
Mdu phan benfa nhan tieu chay: dugc tfau tfaap sdra, bao quan d mdi trudng v ^ chuyin Cary- Blair, vd chuyin vl phdng thi nghiim di nudi cay, phin ldp.
M I U ngfalu: nia sach chdt ban ben ngoai, liy phin thit cit nliuyin (lg) tdng sinh ttong 9 ml mdi trudng nudc pepton kiira (APW) chiia 2% mudi NaCl.
Mau huylt heo: khoing 5ml/radu dugc liy tgi cdc ca sd giit md, mdu dugc chuyin ve phdng thi nghiem tang sinh ttong 9 ml mdi trudng nudc pepton kilm. Tdt e i cdc logi mau diu dugc nudi cdy tich luy trong mdi tmdng APW tir 8 -12 gia.
2.2 Phuong phap nghien cihi 2 2.1 Phuangphdp nudi cdy
i ml miu dugc tang sinh ttong 9 ml mdi tmdng Peptone kiim (APW) chiia 2% NaCl ii 37"C ttong 24 gid nhira tgo diiu ki|n cho nfadm vi khuin Vibrio spp. phdt triln vd ngin cdn sy phdt ttiin cOa vi kfauin cgnfa tranfa khac.
Phin lap tren mdi ttydng chuyin biet TCBS d 37*'C trong 24 gid: khuin lgc cd tiii cd miu ving faodc xanh tiiy lodi:
- Khuin lac V. cholerae ldn, dudng kinfa khodng 2-3 mm, Idng, cd miu ving, hai phdng, tim dye va xung quanh cd qudng ttdng dye
- Kfaudn lac V. parahaemolylicus v i V.
vulnificus lan, dudng kinh kfaoang 3-4 ram. cd miu tir xanh din xanh duong.
Khudn lac V. mimicus ldn, dudng kinh 2-3 mra, pfadng va cd mdu xanfa. Cdn ddi vdi kfaudn lgc V. fluvialis thi phdng, mdu ving va dudng kinh tir 2-3 mra (Trin Linfa Tfaudc, 2009).
Tgp chi Khoa hgc Trudng Dgi hgc Cdn Tho Phdn B: Nong nghifp, Thuy sdn vd Cong ngbe Sinh hgc: 33 (2014): 61-67 Cfagn kfauan Igc diin fainfa cdy chuyin sang mdi
tiTTong NA saline (SNA): mdi tmcmg khdng cd tinh cfagn lgc, viec nudi cay ttin mdi tnrdng ndy chi nhdm lam tang sd lugng kfauin lgc ciia vi khudn.
Trin SNA kfauin lac Vibrio spp. ttdn, tton, ldng, bdng, mdu ttang siia.
2.2.2 Xdc dinh V. cholerae bdng phdn ung sinh hda
Cdc thii nghiim suih hod quan ttgng de phdt hi?n hay phan bill cic loai Vibrio dugc tfayc hien tiieo quy trinh ISOATS 21872-1:2007 (E).
Chgn khudn lgc tten mdi ttudng SNA dl thii nghi|ra oxidase duang tinh (+); TSI duang tinh Bang 1: Tdm tat tfau nghiem khang fauyit thanh
(+); khdng smh faoi vd khdng sinh H^S; Indole duang tt'nli (+) vi di ddng (+). Thir tt'nh ua man cua Vibrio spp. d cdc ndng do mudi NaCl khic nhau (0%, 2%, 6%. 8% va 10%), V. cholerae mgc tdt ttin radi tmdng cd mudi tir 0 - 2%; V.
parahaemolyticus mgc tdt tren mdi truong cd raudi tii2-8%.
2.2.3 Phuangphdp dinh danh bdng phdn ung huyet thanh hoc
Khing huylt thanh chin dodn vi khudn V.
cholerae bao gdm: Khang huylt thanh ta da gia 3 chung: Inaba, Ogawa vd 0139; khdng huylt thanh ta don gia Inaba; klidng huylt thanh la don gid Ogawa.
V. cholerae Khing
nguyin
Khang huyet Khing huyet Dung thanfa Inaba thanfa Ogawa muoi
dich sinfa ly Ket luan V. cholerae
V. cholerae V. cholerae V.cholerae
ChungInaba Chiing Ogawa
Chiing da gid (Inaba + Ogawa + 0139) Chiing khdng dac hieu
Thir nghiim ngung kit huylt thanh dugc diing de xdc dinfa cdc ddng Vibrio cd cdc bilu faien khdng nguyin chuyin bi|t. Tmong hgp V. cholerae cd thi phan bill dugc cdc ddng V. cholerae 0 1 vi V.
cholerae non 01 (khac OI) (Tran Linh Thudc.
2009) nhu sau:
Thu nghiim vdi khang fauyet thanfa da gii:
nhd mgt gigt khing fauyet tfaanh da gid len phien kinli sach va mOt nudc mu6i sinh l;^ lln mdt vi tti khic ciia phiin kinh. Dimg que cay vd triing chuyin vi khudn lln 2 gigt dung dich ttin, tan diu vi khudn vio ttong gigt dung dich ttin. Kit ludn duang tinh khi cd hiln tugng ngung kit.
- Xdc dinh kiiu khang nguyin OI: diing khing huylt thanh dan gia Inaba vd Ogawa. Khdng huylt thanh Inaba (+) khi khdng nguyen chi ngung ket vdi khing huyet thanh Inaba vi khdng ngung kit vdi cdc nhdm khac hay dung dicfa raudi sinh ly.
Khing huylt thanli Ogawa (+) khi khdng nguyen ehi ngung ket vdi klidng huyet thanh Ogawa v i kfadng ngimg kit vdi cae nhdm kliic hay dung dich muoi sinh l^.
Phdn ling khang nguyen la (-) khi chiing khdng ngung ket vdi cac nhdm khang huyet thanh neu ttin va khdng ngimg kit vdi dung djcfa raudi sinfa ly.
2.2.4 Xdc dinh su nhgy cdm vd de khdng khdng sinh cua vi khudn Vibrio cholerae
Nguyen ly: Cac chiing vi khuin sl nhay cam vdi cdc loai khang sinh d miic do khac nhau vd chiing the hiln su klidc nhau dd bang dudng kinh vung lie chl d xung quanh khoanh gidy kfaang sinh kfai cd su tilp xdc giiia vi kfauan vd kfadng sinfa.
Chgn 05 loai khdng sinh thich hap ddi vdi vi khudn dudng rugt thudng dugc sir diing di diiu trj b$nh td.
Phuangphdp ldm khdngsinh do
- Mdi tmdng lam kliang sinh dd: mdi tmcmg Mueller Hinton Agar (MHA),
- Dieu chl canh khuan: sau klii thyc hien dinh danli bdng phan iing sinh hda, vi khuan Vibrio spp.
dugc ciy lgi tten SNA, u am d 37°C trong 24 gid.
Dimg que cdy vd trimg chuyen khudn lac tten SNA vao dng nghiem cd chiia 2 ml dung djcfa nudc mudi sinh ly dl ttong hi ara sau 30 phut - 60 phiit lay ra lie diu vi so sdnh dg dye ttong dng n ^ l m bdng do dye ctia dng nghiim chiia dung dich chudn Mac Farland 0,5 (cd sd Iugng vi khuan 10*/ml).
(1) Trdi huyin dich vi khudn lln mat thach;
(2) Dit khoanh giiy khing sinh tilp xiic phing vdi mat thgch, sao cho khoing each cdc dia kfadng sinh tii 20 - 24 mm; (3) Lit ngugc ddy fadp tfaach li a 37''Ctirl8din24gia.
Tap chi Khoa hgc Tnrang Dgi hgc Cdn Tha Phdn B: Nong nghifp, Thuy sdn vd Cdng nghi Sinh hgc: 33 (2014). 61-67 ducmg tinfa vdi V. cholerae Id 10.7% (Nguyen Thi Xudn Trang et ai, 2012)
Tren ngudi: qua 40 miu phdn tiiu chiy duge thu tiiip tgi benh vien da khoa Trd Vinh, khdng co miu duong tinh vdi vi kfauin td Vibrio spp. tgi thdi diim khio sat.
Theo kit qud ngfailn ciiu ihig dung mdt sd ky thuit chin doin nhanh V. cholerae gay tiiu chdy cap tai tinh TTidi Nguyin nim 2009 tt^n 270 radu binh nhdn tieu chdy cip cd trieu chiing lim sdng nghi mdc benh ti cung chi phdn lap dugc 5,93%
binh nhan ducmg tinh vdi binh td (Phgm Thi Vfi, 2008), nguyin nhdn gay tiiu chdy ciia nhiing binh nhin tieu chiy ndy cd thi do tdc nhdn gay binh khac nhu do vi khuan E.coli, Salmonella...
3.2 Ti II nhilm Vibrio spp. tren ngheu d fauyen Cau Ngang va Duyen Hai
Ti ll nhilm Vibrio spp. tren nghiu cda huyin c i u Ngang va huyin Duyen Hai dugc thi hien qua bang sau:
Bdng 3 : Ti le nhiim Vibrio spp. trin ngfalu tai 2 Huyen
Bgc vd nhgn dinh kit qud
Sau khi li 18 din 24 pa, dgc kit qua cdc fadp tfaach, vung lie chi se la radt vdng ttdn xung quanh khoanh giiy khang sinh khdng cd vi khuan rage v i tiiiy dugc bdi^ mdt thuang. Do ducmg kinb vimg lie chi, ki c i dudng kinh khoanh gidy kfaang sinh va dgc kit qua dya vdo bang tiiu chuin ddnh gia sir nhay cim ddi vdi khing sfaih ciia vi khuin dudng mdt (CLSI, 2010). Cd 2 ttudng hgp xdy ra:
Niu xung quanh dia khing sinh kfadng cd vdng vd khudn (thgcfa due din tan mep dm khdng sinh), kit ludn vi khudn khang vdi khang sinh do.
Niu xung quanh dia khdng sinh cd mdt vdng ttong sudt, dd Id vdng vd khuan, (vl khuan khdng rage dugc), kit lugn vi kfauin nhay vdi kfaang sinh dd.
Xir ly so li|u: Xu ly kit qud bdng chucmg trinh Excel; Minitab 14.0
3 KET QUA VA THAO LU^N 3.1 Ti le nhiem Vibrio spp. tren cic loai mau phan lap
Ti Ie nhiem Vibrio spp. tten huylt heo dugc phin lip li 4%. Cd nlueu nghien ciiu cfao tfady ngudn nudc Id yeu to lay lan mam benh. Id dieu kiln dl vi kfauan tdn tgi v i phit tin. Nhilu qudc gia tten the gidi ciing cd nhirng nghiin ciiu liln quan din ngudn nudc gdy d nhiem, nudc ddng vai ttd quan ttgng va luu trii mim binh, hon 50% ngudi bi nhiera vi kfauin V. cholerae la do su dyng ngudn nudc bi d nfailm (Joao P.S. Cabral, 2010).
Bang 2: Tdng hgp ti II nhiem Vibrio spp. trin cdc loai mau
So mau DuoTig tinfa^=^=^^
kiim tra S6 mdu Ti II (%)^
Huyen So mau Duung tinh. -
kiem tra So mdu Ti !$(%) Duyen Hdi
Cau Ngang
80 80
05^
11"
6.25 , 13.75 Tdng
Loai mau
Huyet heo 100 04 4,0 Nghiu 160 16 10,0 Phdn b|nh nhin 40 0 0
Tdng 300 20 6.7
Ti II nhilm Vibrio spp. tten nglilu dugc phdn lap d 2 huyen Ciu Ngang vi Duyen Hai Id 10%, nghiu la loii nhuyen tfal dugc nudi pho biin d 2 huyen vimg ven biln. Nhihig nhi khoa hgc ciing di xdc djnh nguyen nhdn gdy ra su bimg phat dich, vd canh bdo ngudi din vi sy nguy hiira liln quan din vile tiiu tiiu ci s6ng va cdc lodi thuy san ddc bilt la nhuyen thi (CDC, 2001-2009). Kit qui niy cflng phil hgp vdi kit qui kfaao sat ttin nhuyen the d Ddng Nai vd Thdnh pfao Hd Cfai Minh, ti 11
Ghichu: a,bkhdc biit khdng co y nghTa thong M Qua Bdng 3 cfao thdy ti le duong ti'nh vdi Vibrio spp. trin nghiu ciia huyen Cau Ngang la 13.75%
cao hon so vdi ti le ducmg tinh vdi Vibrio spp.
trin ngfalu ciia hi^'ln Duyen Hdi Id 6,25%, tuy
" nfajen sv chinfa l?ch ndy khdng cd ^ nghia thdng ke
•(p"=0,lll).
Ddc tinh cua vi khuin Vibrio spp. thich nghi vi tdn tgi dugc d mdi truong nuac cd ndng A^ muil.
thiefa hgp 2%, 3% vi 4%, 6% va 8%. vi khuin hien diln pfad bien d biin, cdc ciia sdng va cae lodi hdi sdn nfau tdm. cua, sd, ngfalu, hau (Vd vdn Lugng, 2009). NguOT Gl bi nhilm Vibrio spp. khi an sdng hoic chua niu chui cdc loai hdi san tten hodc udng hay tiep xuc cdc logi nudc cd nhiira vi khuin Vibrio spp. CJ Khdnh Hda (Vi|t Nam), tii nam 1997 den 1999 cd nhiiu ca nhilm V. cholerae. va Khanh Hda cung la vimg ven biin, thiic dn stt dyng da s6 la hdi sdn. Theo bio cdo tiiudng niin eua Trang tam kiem soit djch b|nJi thi gidi ndm 2011 (CDC), tinic an hii sin ciing dugc cfaiing minfa vi ti ll nhiem V.cholerae a nhiiu nudc trin thi
Tgp chi Khoa hpc Tnrdng Dgi bgc Cdn Tha Phdn B: Nong nghifp, Thuy sdn vd Cong nghf Sinh hgc: 33 (2014): 61-67 gidi; hdu 65%, tdm 52%, cua 29%, nghiu 11%
(DePaola,1981).
3.3 Ti le nhiem Vibrio spp. frln huylt faeo tai mot sd fauyen
Kit qua (Bing 4) cfao t h ^ tgi ca sd gilt md faeo su dyng ngudn nudc tir mdi tmcmg nudc sdng thugc huyen Chdu Thdnh cd 20% sd mlu dugc phan lip nhiem vi khuan Vibrio spp. tt' II nay cui^
kha cao so vdi ti II nhiera da dugc phin lap ttin nghiu ciia eic huyin (10%). Nfaiing kit qud gidm sat su luu hanh ciia V. cholerae 0 1 , 0139 thdng qua cdc chi thi sinh hgc tai miin Nam Viet Nam 2012 - 2013 cho thiy ti 11 hien diln Vibrio 0 1 , OI39 cd khd ndng tdn tai trong thyc phim tuoi song vd nudc sdng tai Thinh pfad Hd Chi Minh vd Bin Tre nhung khdng raang dgc td ta, ddy Id nguy CO tilm ting ciia su tai biing phdt binh (yhdp, 2013).
Bang 4: Ti II nhiim Vibrio spp. trin huyet heo tgi mgt so huyen
Sd mau Duong tinh nuyeu
Duyen Hai Cau Ngang Chau Thanh Cang Long T.Pho Tra Vinh
kilm tra 20 20 20 20 20
So mau 0 0 04 0 0
T i l e 0 0 20 0 0 3.4 Ket qua xdc djnh type huyit thanh phd bien gdy b^nh tai Tra Vinfa
Cfaung tdi tiln faanh ngung kit khdng huylt thanh da gii vi don gii de xac djnh nhdm huylt thanh cd khd ndng giy binli tten ngudi.
Bang 5: Ti II nfaiem Vibrio cholerae theo type huyit thanh
So mdu duong tinfa ( n = 2 0 ) Duong Ti le
tinh (%) Type huyet thanfa
Inaba + Ogawa + 0139 Inaba (dan gid) Ogawa (dan gid)
3 15 2 10 2 10
Kit qui cfao thiy ed 15% mdu duong tinh vdi kfaing huyit tiianh da gid (0139, Ogawa, Inaba).
10% mdu duong tuifa vdi khdng huyit tiianh don gia Ogawa va 10% raiu duong tmh vdi khing huylt thanh dan gia Inaba. Nhiing chiing khdng ngung kit vdi khing huyit thanh OI vd 0139 se duge xic dinh ttong cac nghiin ciiu tiip theo.
Theo Garg P et ai (2003) V. cholerae ndm ttong nhdra eac vi sinh vdt thudc he sinh tbdi Id mdi tmdng nudc, cdc chiing thudc 01 vi 0139 dugc phin lap ft so vdi cac chimg non - O i vi non - 0139 li nhirng nhdm khang huyit thanh khdng ngung kit (NAG: nonagglulinable), tuy nhiin cd mdt vai chiing ciia V. cholerae khong ngung ket vdi khang fauyit thanh 01 nhung vdn giy binh va ed trieu cfamig tiiu chiy (Nicholas A et al, 2000).
Ci Indonesia, faon 20%i cae benh nhilm trung tiiu chdy li do nfaiem Vibrio spp. Theo Lesmana M et al (2002), nlidm vi kliudn Vibrio spp. cd till phat triln ttong mdi tmdng thach raudi mat-sucrose thiosulfate cittate (TCBS) diu co khuin lgc mau ving va khdng ngung kit vdi khdng huylt thanh V.
cholerae 01 (NAG: nonagglutinating).
CJ Viet Nam, tii nim 1979 din 1981 cdc ca binh ta chu yeu la do biotype EI Tor, serotype Ogawa; tir nam 1982 din 1990 cac ca benh td diu nhiem serotype Inaba; nhiing nim sau 1990 cac ca benh diu do serotype Ogawa (Joao P.S. Cabral, 2010). Thang 10 nam 2007, cic tmong hgp tieu chiy cdp gay ra bdi bien ddi gen vi khuin Vibrio cholerae 0 1 , Ogawa biotype El Tor vd cd diln (Nguyen era/., 2002).
3.5 Tinh nfaay cim vd dl kfaang khang sinfa cua vi khuan Vibrio cholerae
Chiing tdi tien hanh trin 05 loai khing sinh thudng sii dung di dilu tri benh ta nhira danh gid tinh nhay cara va dl khdng khdng sinfa ciia 20 chiing vibrio spp. cd 03 chiing ngung kit vdi khdng huylt Ihanh ta da gii vi 04 chiing ngung kit vdi khang huyet thanh ta don gia Inaba vd Ogawa, cd 13 chiing khdng ngung ket vdi khang huylt thanh nlidm 01 vaOI39.
Bang 6: Ti l | nfaay cam va dl kfaang kfadng sinfa ddi vdi Vibrio cholerae
Khdng sinh Ky failu So man kilm t r a - Nhay cam Trung gian Khdng Sd mdu (%) Sd mau (%) Sd man (%) Araoxicillin Ax
Tettacycline Te Norfloxacine Nr Cfalorampfaenicol CI Azitfaromycin Az
20 20 20 20 20
0 13 20 14 10
0 65 100 70 50
2 1 0 2
"i 10
5 0 10 25
18 6 0 4 5
90 30 0 20 25
Tgp chi Kboa hgc Tnt&ng Dgi hgc Cdn Tba Phdn B: Nong nghiip Thuy sdn vd Cong nghi Sinh hgc: 33 (2014): 61-67 al, 1997), vdi amoxicillin (Bani et ai, 2007). 0
miin Bdc Viet Nam tii nam 2007 din 2010, tdt ed chung V.cholerae phan lap dugc diu nfagy ydi cfaloraraphenicol (100%), ^k kfadng vdi tetracycline la 29% ^ u u Dat Tran et al., 2012).
Nhimg n ^ l n ciiu cua Devarati Dutta et al (2013) cung cho thdy hdu hit nhihig cfaiing khdng ngung kit (NAG) diu di khdng vdi nalidixic acid (57.6%), ampicillin (55.5%), vi nfagy cdm vdi gentamicin (96%), tettacycline (80%), va chloramphenicol (80.4%).
Ngfailn ciiu budc dau cfao thiy 100% eac chiing Vibrio spp. nhay cim vdi norfloxacine, 70% vdi chloramphenicol. 65% vdi tetracycline, 50% vdi azithromycin; de khdng vdi amoxicillin la 90%. vdi tetracycline Id 30%, voi azithromycin Id 25%, kit qud nghiin ciiu cua chiing tdi phu hgp vdi nghiin ciiu ciia Nguyin Thi Xudn Trang et al. 2012.
Nam 1995 vi khuin V. cholerae nfagy vdi tettacycline va chloramphenicol, nhung den nam 2000 vi khuin V. cholerae lai de khang vdi tettacycline vi chloramphenicol (Vijayalakshmi et
JBNhgy w
..
1
3 ^
a L m
Bieu do 1: Su nhay cam va de kfadng kfaang sinfa cua cac cfaung Vibrio spp.
4 KET LUAN
Kit qua kiim tta ti 11 nhilm Vibrio spp. ttin 300 mdu (miu ngheu, huylt heo, phin benh nhan) d Trd Vinfa cfao tiiay tdn sd xudt hiln Vibrio spp.
trin ngheu la 10%, man huylt heo la 4%, tten benh nhin tiiu chdy chua phdn lip dugc Vibrio spp. Vi khudn phan lap nhay vdi norfloxacine (100%), vdi chloramphenicol (70%). tetracycline (65%), azithromycin (50%); dl khdng vdi amoxicillin (90%), vdi tetracycline (30%), vdi azithromycin (25%). Cd 15% mlu duong tinfa vdi khang huylt thanh da gid (0139, Ogawa, lnaba),10% mdu duong tinh vdi khing huylt thanh don gii Ogawa vd khdng huylt thanh don gid Inaba.
Can phai cd nhung ngfaien ciiu tiep theo dl cd nhiing kit qud cu the ve cac loai cua Vibrio spp. vd ti'nh di khang khing sinh ciia chiing.
LOfI CAM T4.
Chin thanh cim on Ban Giim failu. Ban Idnh dao Khoa Ndng ngfailp-TTiiiy sdn Tmdng Dai hgc Trd Vinh eiing cae ddng nghiip ttong bd mdn Chin Nudi Thii y da tgo dilu kiln vl v|t chit, tinh thdn ttong sudt qui ttinfa tdi tham gia dl tdi.
Chin thinh cdm an Ban lanfa dao Chi eye Thii y tinh Tra Vinh da tgo dilu kiln cho chiing tdi tham gia ldy raau tgi cdc ca sd gilt rad ttong dja ban Tinh.
Chdn thdnh cdm an cic anh cfai em Bd mdn Vi sinfa Khoa Ndng nghiep & Sinh hge LTng dyng, Tmdng Dai hgc Cdn Tha; anh ehi em Bg mdn Vi sinh, Truong Dai hgc Y Dugc Cin Tfau; anfa^chi- Khoa xet nghiim benh vi|n Da khoa Tnmg uong"
Cin Tho di tgo dieu kien, gitip da tdi hodn tiidnh nghiin ciiu de tdi niy.
Cam on cdc era sinh vien Khoa Ndng nghiep &
Sinh hgc tfug dung, Tmdng Dai hoc Cin Tho cac em sinfa vien ldp DA09BTY Khoa Ndng ngfaiep - Thiiy sdn Tmdng Dai hgc Tra Vinh da hd ttg toi ttong then gian qua.
TAI LIEU THAM KHAO
• Nguyen Thi Xudn Trang, Nguyin Ngoc Tuan (2012). "Tin sd xuit failn Vibrio cbolerae trin tdm vi nhuyin thi, xac dinh i Serogroup01,0139viBiotypeciia V. } cholerae bdng ky tiiudt Multiplex - PCR " ^ Khoa hoc ky thudt Thdy l9(3):5i-55
Tgp chi Khoa hgc Tntang Dgi hgc Cdn Tho Phdn B: Ndng nghifp. Thin> sdn vd Cong ngbe Sinh hgc: 33 (2014): 61-67 2. Phgm T h i Vu (2008): "Ngfailn cdu ting
dung mdt sd kJ tfauat chin doan nfaanh Vibrio cholerae giy dich tiiu chiy cap tai tinh TTidi Nguyin ndm 2008". Lugn vdn thac sTSinh hgc: 29 - 31.
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& Colombo, M. M. (2007). Molecular characterization of ICEVchVieO and its disappearance in Vibrio cholerae 01 strains isolated ui 2003 in Vietnam. FEMS Microbiol Lett 266(l);42-8.
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http://dx.doi.org/10.1016/S0732-8893 (02)00373-5.
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