Tap chi Khoa hoc - Cong nghe Thuy sin So 4/2013
KET QUA NGHIEN COU DAO TAO SAU DAI HOC
NGfflEN c i r u ANH m / d N G CUA LYSINE, PROBIOTICS DEN TOC DO SINH TRirdNG VA CHAT LlTONG THIT GA TA
STUDY ON EFFECT OF LYSINE, PROBIOTICS ON DEVELOPMENT RATE AND "GA TA"MEAT QUALITY
Nguyen Tten Toan', Do Van Ninh' Ngay nhin bii: 05/01/2012; Ngay phanbi?n thong qua: 22/8/2013; Ngay duyet dang 10/12/2013
T 6 M TAT
Anh huang cua vi$c bo sung lysine vd probiotics den tdc dp smh truang vd chdt lugng th\t gd ta dugc nghien cim.
Hdm lugng lysine dugc bo sung vdo thuc an aba muc 0,8,1 vd 1,2%. Probiotics dugc su dung a cdc muc 0,2.0,4 vd 0,6%.
Hai thi nghidm dot chung cSng duac tien hdnh song song de ddnh gid hi?u qud ciia vi?c bo sung lysine/probiotics. trong do mgt mau khong su dying lysine/probiotics vd mdu con lai su dung thuc dn ihuang mgi Kit qud nghien cuu cho thay ho sung lysine 0.8 -1.2% giup tdng toe do sinh truang, hieu qud chuyen hoa thirc dn cung nhu chdt lugng ca thit tot han so vai mau doi chung khong b6 sung lysine. Viec bo sung 0,2-0,6%probiotics trong khau phdn thuc dn gidp tdng tdc da sinh trudng vd hieu qud su d{ing thirc dn so vdl mdu doi chung khong bo sung probiotics Ket qud nghien ciru ndy ma ra liem ndng CO the su dung lysine vd probiotics trong khdu phdn thucdn chan nuoi gd nhdm tdng loc do sinh trudng, hi^qudsu dung Ihuc an cUng nhu cdi thi^n chdi lugng Ihft.
Tit khoa chat lugng, gd ta, lysine, probiotics, sinh trudng ABSTRACT:
Effects of lysine and probiotics supplement on development rate and "Ga ta " meat quality were investigated Lysine contents were added on diets at three levels, including 0.8, 1 0 and 1 2% Probiotics was used at 0 2, 0.4 and 0 6%. Two control experiments, including one experiment which was without lysine or probiotics addition and the remaining one was carried out using commercial diet, were conducted in paralell to evaluate effectiveness of addition of lysine or probiotics.
Research results indicated that supplementation of lysine, arranging from 0.8 to 1 2%, increased development rate, feed conversion effectiveness as well as meat quality compared to the control sample in which lysine was not added. Results were also recorded similarly toward the addition of probiotics to diet. Supplementation qf probiotics from 0.2 to 06%, increased development rate and feed conversion effectiveness compared to the control sample which was not supplymenled with probiotics. Our findings revealed potential of using lysine and probiotics as supplements in chicken diet m order to increase of development rate, diet conversion effectiveness as well tis improvement of meat quality.
Keywonls: quality. Gala, lysine, probiotics, development
I. O^T V A N Dt ttiyc phim dang ed ehieu hudng gia tang. Vifc lgm Gd Id mgt trang nhung d i i tijang gia d m dugc dyng cdc chit kich Uiid tang tiuang, ede chit khdng nudi khd pho biln d Vift Nam. Trong nhung nam sinh trang qud trinh ehan nudi gia d m da din 6h gan day do nhu d u lieu thy gia d m vd ede sdn nhilu nguy ca mit vf sinh an todn ttiyc pham. Cdc phim tif gia d m tang mgnh nen da xult hien nhieu chit kich thieh hay khdng sinh thudng dugc b i sung trang trgi nuoi gia d m d qui m i cdng nghifp. Tuy vdo thi>c dn nhim giiip d o vft nudi tang trudng nhien, nhung vin de phdt sinh tir qud trinh nudi gia nhanh, ndng cao sipc de khang. VI vfy, xu hudng sir d m , ti^ng dd cd gd, lidn quan din vf sinh an toan dyng chit kich thich tdng tn/dng hay ehit khang sinh
' NguySn Tiln Todn' Cao hpe Cong nghf Sau Ihu hoach 2008 • Tnjdng Dgi hpe Nha Trang
^ TS. D5 van Ninh: Trt/dng Dgi hgc Nha Trang
Tgp chi Khoa hoc - Cong nghe Thuy sdn So 4/2013
trong ehdn nudi gia cam eo chieu hudng gia tang vi lgi idi v l mat kinh te. Dilu dd da dan d i n nguy ca mat an t d n v f sinh ttiyc phim dd duge ghi nhan tiong ttiyc t l . Cae ca quan chuc nang da khuyin cao va cd nhdng qui dinh nghlim khde nham hgn che viee su dung eae chat kieh ttiich sinh trudng cung nhu khdng sinh tiong chan nuii gia d m .
Bo sung lysine vd probiotics vdo ttianh phin thifC dn d tac dyng ti'ch cyc tiong vifc d i thien toe df sinh trudng, hifu qua chuyin hda thuc an, ndng eao sue d l khang cung nhu chit lugng d a thjt gia d m vd thjt heo dd dugc nghien cuu t)di mgt s i tdc gia T i n Thit San va cpng sy (2010), Trin Quoe Vift vd Dang Thdi Hdi (2010), Le Vdn An va egng sy (2006), Hodng NghTa Duyf t (2006). Nguyin Anh Dung (2010), Trin Quie Vift va cfng sy (2008a), Trin Quoe Vift vd d n g sy (2008b), Phgm Tat Thing va La Vdn Kinh (2006). Tuy nhien, vifc nghidn cuu bo sung lysine vd probiotics ti'ong khau phin thuc an gd ta nuii thuang phim van d n nhieu hgn c h l . VI vfy, mye tieu eua nghidn cuu nay Id: Nghien cieu anh hudng cua vifc t)o sung lysine, probiotics den tic d f sinh ttudng, hieu qua chuyen hda thue dn vd chit lugng cua thjt gd ta nuii thuang pham.
II. D 6 1 TITONG, VAT LIEU VA PHITO'NG P H A P NGHIEN CLTU
1. Vft lifu
Ting so 240 con Gd ta (Gallus gatlus domesticus) 4 t u i n tuoi c i ti'png lugng tmng binh 180 g/con (n = 120) doi vdi ga trong va 165 g/eon
Bdng 1. Khiu phin thiPc dn cho gd trong d c Id thi nghifm (dom vj % so vo'i lysine) (n = 120) doi vdi gd mai dugc tien hdnh nghidn cuu. Cdc thye nghifm dugc l i l n hdnh tgi Trai ga Hda Quang, Phii Hda, Phii Yen. B i sung che pham L - Lysine do Ajinomoto ThaiLand san x u l t C h l pham probiotics dgng bgt do Vien Pasteur Nha Trang san xuat, d mft do vi khuan lO^cfu/g vdi hai chung vi khuan chinh gom: Lactobacillus acidophilus vd Bacillus subtilis. Cdc nguyen vat lifu khdc g i m : Bgt ngd, t i m gao, cam gao, khd ddu tuang, bdt cd, methionine, cystine, threonine, tryptophan, muoi NaCl, premix Vitamin, premix khoang mua tir d c hang ttiuc an eing nghiep tgi Tuy Hia, Phii Yen.
Thue an hon hgp C911 do Cing ty d phan Chdn nudi C P Viet Nam sdn xult, mua lai dgi ly thiJc dn CT Tuy Hda, Phu Ydn.
2. Bo tri thi nghiem
Thi nghiem anh hudng ciia viee bo sung lysine vdo khiu phin ttvjc dn din t i c do sinh tiiwng vd chit Iugng gd ta dugc thye hien h-in 120 con gd con 28 ngdy tuoi, chia thdnh 5 l i , mdi Id g i m 12 con ti^ng vd 12 con mdi, moi con dugc deo so de theo ddi. M i l Id nudi bang 3 ling, m i l long 8 eon (4 tiing va 4 mai), long nuii d kieh thude 1,6 x 1,0 x 1,0 (m). Khau phan thuc dn eho gd d edc l i thi nghifm duge trinh bdy chi lilt trong bang 1. Gd dugc cho dn theo c h l do 3 lln/ngdy, vdo luc 7 gid, 12 gid vd 17 gid.
Thi nghifm anh hudng eiia vifc bo sung probiotics vao khiu phin thue dn 6^t\ t i e d f sinh trydng va chat Iugng gd ta eiJng dugc thye hifn tuang tij nhu tren va dugc ti'inh bdy chi tilt trong bang 2.
STT 1 2 3 4 5 6 7 8 9 10 11 12
Nguyen li^u thi>c an BOt ngd
Tam ggo te Cam 9^0 Kho dau tirang Bdtca Lysine Methionine + Cystine Threonine Tryptophan Muoi NaCl Premix Vitamin Premix khoang
L 6 1 43 11 10 16,6 10 1,2 0,6 0,45 0,2 0,15 0.5 0,5
L6 2 43 11 10 16,6 10 1,0 0,6 0,46 0,2 0,15 0,5 0,5
L6 3 43 11 10 16,6 10 0,8 0,6 0,45 0,2 0,15 0,5 0,5
DC 1 43 11 10 16,6 10 0 0,6 0,45 0,2 0,15 0,5 0,5
0 C 2 Cho §n thijc an
hon hep C 911
TRUONG OAI HOC NHA TRANG • 145
Tgp chi Khoa hoc - Cong nghe Thtiy sdn So 4/2013
STT 1 2 3 4 5 6 7 8 9 10 11 12 13
Bang 2. K h i u phan thi>c an cho gd trong c i c Id thi nghidm (dom vj % so v 6 i probiotics) Nguydn li#u thiJc Sn
Bot ngd Tim gao te Cam gao Kho d#u tirang B$tca Lysine Methionine + Cystine Threonine Tryptophan Muoi NaCl Probiotics Premix Vitamin Premix lihoSng
L61 43 11 10 16,6 10 1 0,6 0,45 0,2 0,15 0,2 0,5 0,5
LA 2 43 11 10 16,6 10 1 0,6 0,45 0,2 0,15 0,4 0,5 0,5
L6 3 43 11 10 16,6 10 1 0,6 0,45 0.2 0,15 0,6 0,5 0,5
DC1 43 11 10 16,6 10 1 0,6 0,45 0,2 0.15 0 0,5 0,5
DC 2 Cho an thuc Sn h6n hep C 911
3. Xac djnh chi s i tdng truvng
Chi s i tdng trudng dugc xdc djnh theo cdng thdc sau: „ , „ ,
Tieu ton thiic an/kg tang trpng =
Trong dd: A: Sinh tm-dng tich lu? (g/con/ngdy).
W^: Khil lugng gd ciia Iln cdn sau (g).
W,: Khii lygng gd cua lan cdn tnj'dc (g).
T: Khodng thdi gian giua hai lan can (ngdy).
4. Xdc djnh hifu qud sir dung thirc an tren m f t do>n vj k h i l lirgng tdng trgng
Tidu t i n luang thCre an ti'dn mgt don vj khii lugng tdng trgng dugc tinh todn theo edng thue:
Liii;mg thtic an sir dyng (kg) Kiioi lirrag lang (kg) 5. Xdc djnh thanh p h i n hda hgc c a ban
Hdm luang nude vd tro duge xac dinh Iheo phuang phap cua AOAC (1990). Hdm lugng protein dugc xdc djnh theo TCVN 4321-1 (2007) si> dyng h f ttiing phd mlu bdn ty dfng Kejidah (Vapodest 45, Gerharit, Germany). Hdm lugng lipid dugc xac dmh theo phuang phdp ciia Bligh vd Dyer (1959).
6. Hdm lygng a xit b i o
Phdn t i d thanh phin axit beo dugc thye hifn tgi Vifn Cdng nghe sinh hpe vd Mil tiudng, Tnj'dng Dgi hoe Nha Trang, su- dyng hf thing sic ky khi GC d a hang Agilent (model 6890A plus, USA). Axil beo duge phdn tich bing eft HP-FFAP (30 m x 0,25 mm, USA).
Khi mang dugc su dyng la Nito Phan tich nay dugc thye hifn trong ba Iln lap Igi, kit qua bdo cdo Id gia trj trung binh.
7. Phirong phap xi> 1;^ so l i f u
Cdc thi nghifm dyge b i tri Ifp lgi it nhat ba Iln de ddm bdo ttiyc hifn phan tieh ANOVA. S l lifu dugc xu ly iren phin mIm SPSS 16.0. Sy khdc bift
e i 'j/ nghTa giua cac k i t qud duge ddnh gid d mirc p < 0,05 ap dyng lieu chuin Ducan.
111. K^T QUA NGHIEN CI>U VA T H A O L U A N 1. Anh h u ^ n g cua lysine trong khau phan thirc an d i n t i c dp sinh triri'ng
Anh hudng eua lysine trong khiu phin thipc &n den toe d f tdng trudng dugc trinh bdy ti^ong hinh 1.
Kit qua cho ttily cac Id gd cho an vdi khiu phan ttiilc dn cd bo sung lysine tiF 0,8 - 1,2% eho thiy tie (If tdng tnfdng cao ban mlu dii ehung khing bo sung lysine (DCI). T i c do tang trudng tmng binh cua cdc l i gd an thuc an ed bo sung lysine tir 0,8, 1,0% vd 1,2% sau 4 - 8. 8 - 12 vd 12 - 16 tuin tuii iln lugt Id 8,9, 20,3 vd 18,0 g/eon/ngay Trong khi dd, dii vdi m l u DCI Ian lugt la 8,4,19,6 va 16,3 g/con/ngdy Dli vdi lo ga sir dyng thuc an ting hgp 0 911 (DC2) thi t i c d f tang tiirdng cao han cac Id gd d n lgi vdt tic d f tang ttudng bung binh sau 4 - 8, 8 - 12 vd 12 -16 tuin tijii iln luat la 9,4, 20,8 va 19,3 g/con/ngdy Kit qud nghien ci>u cung eho Ihly ring mf c dii thirc dn d b i sung them lysine cdi thien dugc tic df tdng hiring ciia gd, hjy nhien so vdi thirc dn ting hgptrSn thj tnrdng till t i c dp tang tnrdng v i n thip hon mft chill. Dieu ndy cd the dugc Iy gial Id do thdc dn ting hgp ngodi vifc bo sung ttiem lysine trong cdng thirc d the cdn chua ttiim nhirng y l u t i tdng ti-gng khSc nhu chat kich thieh sinh tnjdng.
Hinh I. Anh hir^g cua lysine trang khiu pftSn thih; in tsio
WU'
t^G d$ tiing triring
Tgp chi Khoa hoc - Cong nghe Thuy sdn So 4/2013
2. Anh hipimg ciJa lysine trong k h i u phan thirc dn d i n h i f u qud c h u y i n hda thirc an
Hifu qua ehuyen h i a thue an cOa cac Id ga cho an thuc an cd b l sung lysine eao ban Id d i i chirng (DC 1). nhung thip ban l i DC 2 {hinb 2). Hifu qua sir dyng thirc an trung binh cCia cac l i gd thi nghifm cho thirc dn b i sung them lysine ti> 0,8 - 1,2 % thuc dn sau 4 - 8, 8 - 12 va 12 - 1 6 tuan iln lugt la 2,84, 3,08 vd 4,17 kg thirc an/kg tang trpng. Trong khi dd, dii vdi 16 DC 1 Id 3,19, 3,33 vd 4,83 kg thue dn/kg tang b^ng va doi vdi Id DC 2 la 2,49, 2,88 vd 3,70 kg thiPC an/kg tang trgng. Hifu qua ehuyen hda thire an cila d c l i gd thi nghiem eo bo sung them lysine trong khiu phin thirc an cao han I i dol chirng khdng b i sung them lysine d t h i dugc 1^ gial Id do tde dyng tieh eye eiia lysine giiip vft nuii dn ngon han vd kich thich sy chuyin hoa thi>c an t i t han. Lo ga DC2 d hifu qud chuyen hda thirc an t i t han d e 16 cd t l i sung thim lysine d the Id do thde an ting hgp cung d chira lysisne, ngodi ra d n e i them eac yeu t i kich Uiich tang tnjdng khdc.
Hinh 2. Anh hniVng cda lysine trong khiu phin thirc in d^n hifu qui chuyeii h6a thih: in 3. Anh hud'ng cua lysine trong k h i u phan thi>c an d i n c h i t luyng thjt gd
Chat lugng thjt gd dugc ddnh gia dya vao cdc d t t i i u hda hge, bao g i m thdnh phin hda hpe ca ban
vd thanh phan a xit beo nhu dugc Irlnh bay trong hinh 3. Nhin chung, vifc si> dung thdc an cd b i sung them lysine khdng dnh huong ddng k l d i n thdnh phan hda hgc ca bdn ciia thjt gd (p > 0,05) so vdi edc mau d i i chirng, ngogi tru hdm Iugng lipid (hinh 3a). Ham lugng nude, tro, protein vd lipid cda thjt gd cho an thuc an ed bo sung them lysine 1% (L-10) lan lugt Id 67,49%, 0,99%, 22,61% vd 7,75% Trong khi dd, dol vdi m l u DCI Id 66,93%, 1,05%, 22,98% vd 8,45%, doi vdi mau DC2 Id 68,30%, 0,95%. 22,52% vd 7.69%.
Ket qud cung eho thiy gd an thde an cd bo sung thdm lysine chi 10 g/kg cd hdm lygng ehit beo cao han ddng ke ( p < 0,05) so vdi gd an thirc an ting hgp. Dilu ndy d t h i dugc Iy gial Id do thirc an an ed bo sung thdm lysine chi lOg/kg cd thdnh phan dinh dudng it hoan hao ban thde an ting hgp nen Idm tdng sy tich luy chit bdo. Ddy Id mgt yeu to khdng mong muin Idm gidm chit Iugng ciia thjt gd.
Phdn tich thanh phin axil beo cCia thjt ga (hinh 3b) cho thiy thjt gd eho dn thuc an c i b i sung lysine 1% (L-10) d SFA (1,95%) thip han DC 1 (2,04%). Nguge Igi, MUFAvd PUFA eiia m l u L-10 cao ban DC 1 vdi gid trj cOa ehung lan lugt Id 0,86% va 1,68% so vdi 0,72% vd 1,66%. Dieu ndy eho t h i y rang thit ga m l u L-10 c i ehat lugng t i t ban DC 1. Kit qua nghien cuu cOng eho thay gd dn thipc an t i n g hgp ed chit lugng thjt t i t han L-10 vd D C I dya vdo chf s i SFA, MUFA va PUFA.
Dieu nay d the dugc \y gidi Id do thue an tong hgp chua tuang d i i dly du cdc ehit dinh duang thiet y l u de vft nuii sinh trudng, phdt triln t i t ding thdi ddm bao dugc chat iugng ca th]t.
Hinh 3. Anh hu*ng cua lysine trong khiu phan thire ia den chat lugng ciia thjt gi
TRUONG OAI HOC NHA TRANG • 147
Tgp chi Khoa hoc - Cong nghe Thiiy sdn So 4/2013
4. Anh h u v n g cua probiotics trong khau phan thi>c dn den toe dg sinh truimg
Vifc b i sung probiotics vdo khiu phan Uiirc an vdi hdm lugng ti> 0,2 - 0,6% d i thifn t i c do sinh tnrdng so vdi mdu doi chirng khdng bo sung probiotics (hinh 4). Toe do sinh tardng trung binh cLia d c Id gd dn thire an cd bo sung probiotics 0,2 - 0,6% sau 4 - 8, 8 - 12 vd 12 - 16 tuin iln Iirgt Id 9,74, 20,31 vd 18,28 g/eon/ngay. Trang khi do, d i i vdi l i gd khing b i sung probiotics (DCI) Id 9,04, 19,94 va 17,55 g/con/ngdy d i i vdi I i gd cho an thuc an long hgp (DC2) Id 9,86, 20,97 va 19,16 g/eon/ngdy. K i t qud nghiin euu cho thay probiotics d tdc dung giup gd tang trying manh ban. Mlu ga d Id DC2 cd t i c dg tang trudng mgnh ban cd the Id do tiidc an ting hgp eung ehira d c chit kich thieh tdng truing ding thdi edn ed edc thdnh phin dinh dudng quan trpng khac.
Hinh 4. Anh huong cua probiotics trong khSu phin thuc in den t6c d$ sinh truomg
5. Anh hiFOmg cua probiotics trong k h i u p h i n thuv dn den h i f u qua sir dung thirc an
Hifu qua chuyin hda thue an cua ede l i gd eho dn thde dn cd b l sung probiotics eao ban l i d i i Chung (DC 1), nhung ttilp ban Id DC 2 (hinh 5).
Hifu qud chuyen hda thuc an tnjng binh eua cde l i ga thi nghiem cho thirc an b i sung them probiotics tir 0,2 - 0,6% sau 4 - 8, 8 - 12 vd 12 - 16 tuin lan lugt Id 2,61, 2,93 vd 3,97 kg tiiirc an/kg tang trgng.
Trong khi dd, d i i vdi Id DC 1 la 2,95,3,13 va 4,36 kg thire an/kg tang ttgng va doi vdi I i DC 2 Id 2,53, 2,80 vd 3,69 kg ttiuc dn/kg tang ti^ng. Hifu qua chuyen
hia thuc an d a cdc l i ga thi n g h l ^ d b l sung thim probiotics trang khau phin thuc an cao hon Id d i l chung khdng bo sung thdm probiotics cd the dugc ly giai Id do tdc dung tich cyc d a probioties giiip vdt nuoi an ngon ban, tdng sirc de khdng va kidi thich su chuyen hda thuc an tot ban. L i gd OC^
CO hifu qua d u y l n hda thuc dn t i t ban cdc l i c i bl sung them probioties ti> 0,2 - 0,6% d the Id do trong UiifC an long hgp cOng cd ehira probiotics, ngodi ra cdn cd t h e m R c y l u to kich fi
Hinh 5. Anh hirang ciiH prohiotics trong khiu p 9
!. den hifu qua chuyin hiia thvc an T^
IV. K ^ LUAN VA KI^N NGHj
Bo sung lysine tu 1-1.2% vdo thuc dn giup ldng t i c d f sinh trudng eiia gd ta nuoi thuang phim 10,4% so vdi mdu doi ehi>ng khdng b l sung lysine vd hf s i tieu ttiy thuc an trdn 1 kg tang Irpng gidm 13,7% sau 16 tuin nuii. Ben eanh d i , vifc b i sung lysine cung lam cai thifn chit Iugng ttijt gd. K i t qud luang ty cung dugc ghi nhgn khi b6 sung probiotics vdo khau phan thirc an. Vifc bo sung 0,6% probiotics trong khiu phan thue an giiip tang t i c dg sinh trudng 4% va he so tiiu ttiy thdc an tren 1 kg tdng trpng gidm 9,8%. Kit qud nghien ciru nay ch! ra tiem ndng su dyng lysine va probiotics trong khiu phan thuc an cho gd null thuang pham d l d i thifn t i c do sinh tnrdng, hifu qua chuyin hda thtjc dn vd chit Iugng thjl.
Nhu-ng nghien cuu tiep theo nen duge thye hifn nham ddnh gid dnh hudng k i t hgp cua lysine va probiotics d i n sinh trudng, phdt triln, hifu qud sir dyng thirc an vd chit Iugng ca thjt.
Tgp chi Khoa hoc - Cong nghe Thtiy sdn So 4/2013
TAI LIEU THA.M KHAO Tieng Vift
1 Le Van An, Dao Thj Phugng, Hoang Minh Tuin, 2006. Hi|u qua sit dung lysine va methionine trong khau phan la khoai lang ii nuoi 1cm thit d nong hf, T ^ chi Nong nghiep v^ Phat in6n nong thon, ky 1/6: 52 - 55.
2 Hoang NghTa Duy^ 2006. Anh huong cua cac muc lysine khac nhau trong khau ph4n Ign nai mong cai giai doan chira va nuoi con toi nang suat sinh san cua chung. Tap chi Nong nghiep va Phai iri^n nong thon, ky 2/12: 49 - 52.
3. Nguyen Anh Diing, 2010. Nghien cuu anh huong ciia chS p t i ^ probiotics den liieu qua chan nuoi heo va giam 6 nhiem moi trudng khong khi chu6ng trgi. Ban tin thong tin Khoa hpe va cong nghe, s6 3/2010.
4 Ton Th3t Son, Nguyen Thj Mai, Nguyin Th6 Tu6ng, 2010. Anh huong cua miic lysme trong thuc an dgn kha nang smh truong cua Ign coo lai tir 7 - 28 ngay fti6i. Ta> chi Khoa hpe va Phit trien, lap 8 (1): 90 - 97.
5 Pli^m Tat Thang vi La Van Kinh, 2006. Tac dung va hieu qua cua viec hfi sung probiotics trong thiic an cho Ion thit Tap chi Nong nghiep va Phat trien nong thon, ky I /8 76 - 78.
6 Tiin Quoc Viet va D ^ g Thai Ha, 2010. Anh huong cua cac miic nang lupng trao doi, protem tho va lysine trong khiiu phan den sinh tnrong va hifu qua chuyfin hoa thiic an cua vjt CV super M nuoi thit. Tgp chi Khoa hgc va Phat tnen, tap 8 (2);
277 - 286
7. T i ^ Quoc Vipt, Bill Thi Thu Huyen, Ninh Thi Len, Nguyen Thi Phung, Le Van Huyen va Dao Due Kien, 2008a. Anh huong ciia v i ^ ho sung probiotics veto khau phan den kha nang tieu hoa, tdc dg sinh tmong, hi^u qua su dung thiic an va ty le mac benh heu chay ciia Ipn con va Ion thit. Tap chi Khoa hpe va Cong nghe Chan nuoi, sd 11, thang 4/2008 40 - 47.
% Tran Quoc Vi?t, Biii Th] Thu Huyen, Ninh Thj Len, Nguyen Thi Phyng, Le Van Huyen vii Dao Duo Kien, 2008b. Anh huong ciia v i ^ l>6 sung probiotics vao khau phan den kha nang tieu hoa thirc an, tdc dp sinh tnrdng va hi^u qua su dung tbiic an ciia ga Luang Phupng nuoi thjt. Tgp chi Nong nghiep va Phat trien nong thdn, sd 7/2008: 52 - 57.
TtengAnh
9 Bligh,E G.andDyer,W.J., 1959. Arapidmethodoftotallipidexiractionandpurification Canadian Journal of Biochemistry and Physiology, 37(8): 911-917.
10. Helrich K, editor, 1990. Official Methods of Analysis ofthe Association of Official Analytical Chemists. 5th ed. Arlmgton, Va.: AOAC tac.
TRUONG DAI H O C NHA TRANG • 149
